DE69939896D1 - Methoden zur detektion von nukleinsäuren unter verwendung von zymogenen und dazugehörige kits - Google Patents

Methoden zur detektion von nukleinsäuren unter verwendung von zymogenen und dazugehörige kits

Info

Publication number
DE69939896D1
DE69939896D1 DE69939896T DE69939896T DE69939896D1 DE 69939896 D1 DE69939896 D1 DE 69939896D1 DE 69939896 T DE69939896 T DE 69939896T DE 69939896 T DE69939896 T DE 69939896T DE 69939896 D1 DE69939896 D1 DE 69939896D1
Authority
DE
Germany
Prior art keywords
nucleic acid
methods
target
sample
cymogens
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69939896T
Other languages
English (en)
Inventor
Alison Todd
Caroline J Fuery
Murray Cairns
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Johnson and Johnson Research Pty Ltd
Original Assignee
Johnson and Johnson Research Pty Ltd
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Filing date
Publication date
Application filed by Johnson and Johnson Research Pty Ltd filed Critical Johnson and Johnson Research Pty Ltd
Application granted granted Critical
Publication of DE69939896D1 publication Critical patent/DE69939896D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69939896T 1998-03-05 1999-02-22 Methoden zur detektion von nukleinsäuren unter verwendung von zymogenen und dazugehörige kits Expired - Fee Related DE69939896D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7689998P 1998-03-05 1998-03-05
PCT/IB1999/000754 WO1999045146A1 (en) 1998-03-05 1999-02-22 Zymogenic nucleic acid detection methods, and related molecules and kits

Publications (1)

Publication Number Publication Date
DE69939896D1 true DE69939896D1 (de) 2008-12-24

Family

ID=22134859

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69939896T Expired - Fee Related DE69939896D1 (de) 1998-03-05 1999-02-22 Methoden zur detektion von nukleinsäuren unter verwendung von zymogenen und dazugehörige kits

Country Status (13)

Country Link
US (3) US6140055A (de)
EP (1) EP1062367B1 (de)
JP (1) JP4318856B2 (de)
KR (1) KR100748863B1 (de)
CN (1) CN1167811C (de)
AT (1) ATE414169T1 (de)
AU (1) AU768627B2 (de)
BR (1) BR9908567A (de)
CA (1) CA2321948A1 (de)
DE (1) DE69939896D1 (de)
IL (2) IL164182A0 (de)
WO (1) WO1999045146A1 (de)
ZA (1) ZA991772B (de)

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AUPQ495700A0 (en) * 2000-01-05 2000-02-03 Johnson & Johnson Research Pty. Limited Method for concurrent amplification and real time detection of polymorphic nucleic acid sequences
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
CA2412664A1 (en) * 2000-06-15 2001-12-20 Board Of Regents The University Of Texas System Regulatable, catalytically active nucleic acids
US7125660B2 (en) 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
AU2002355571A1 (en) * 2001-08-09 2003-02-24 Archemix Corporation Nucleic acid sensor molecules and methods of using same
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US6617137B2 (en) * 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
JP4782668B2 (ja) 2003-03-31 2011-09-28 エフ．ホフマン−ラ ロシュ アーゲー 日本脳炎ウィルス血清グループのメンバーを包含する一定のフラビウィルスを検出するための組成物及び方法
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
CN101076537B (zh) * 2003-11-19 2012-07-04 阿莱洛吉克生物科学公司 标记有多个荧光团的寡核苷酸
WO2005085474A2 (en) * 2004-03-02 2005-09-15 Clontech Laboratories Inc. Fluorescent energy transfer labeled nucleic acid substrates and methods of use thereof
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
US20060211018A1 (en) * 2005-02-08 2006-09-21 Applera Corporation Nucleozymes and methods of use
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
WO2006119326A2 (en) * 2005-05-02 2006-11-09 Stratagene California Oligonucleotide probe/primer compositions and methods for polynucleotide detection
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
AU2006302729B2 (en) 2005-10-07 2011-02-03 Speedx Pty Ltd Multicomponent nucleic acid enzymes and methods for their use
WO2007056312A2 (en) * 2005-11-07 2007-05-18 Clontech Laboratories, Inc. Universal reporter tag for nucleozyme assays
CA2631881C (en) * 2005-12-07 2015-03-24 Tibotec Pharmaceuticals Ltd. Methods, plasmid vectors and primers for assessing hiv viral fitness
WO2008001376A2 (en) * 2006-06-28 2008-01-03 Yissum Research Development Company Of The Hebrew University Of Jerusalem Detection of analytes in a medium
DE102006035600B3 (de) * 2006-07-27 2007-11-08 Epigenomics Ag Verfahren zum Nachweis eines Methylierungsmusters
EP1882746A3 (de) * 2006-07-27 2008-03-19 Epigenomics AG Methode zum Nachweis eines Methylierungsmusters
ES2520023T3 (es) * 2006-10-06 2014-11-11 Speedx Pty Ltd Interruptores moleculares y métodos para su uso
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
NZ580129A (en) * 2007-04-05 2012-05-25 Johnson & Johnson Res Pty Ltd Nucleic acid enzymes and complexes and methods for their use
EP2071035A1 (de) 2007-12-13 2009-06-17 Epigenomics AG Vermittler und Verstärkungsverfahren
WO2010147848A2 (en) 2009-06-15 2010-12-23 Rd Biosciences, Inc. Kits and methods for selective amplification and detection of nucleic acid targets
CN101907573B (zh) * 2010-08-17 2012-08-22 复旦大学 一种高灵敏度的集成cmos光学生物芯片
WO2012034130A2 (en) 2010-09-10 2012-03-15 Qiagen Methods and compositions for nucleic acid detection
WO2012142516A1 (en) 2011-04-15 2012-10-18 Becton, Dickinson And Company Scanning real-time microfluidic thermo-cycler and methods for synchronized thermocycling and scanning optical detection
EP2773892B1 (de) 2011-11-04 2020-10-07 Handylab, Inc. Vorrichtung zur vorbereitung von polynukleotidproben
AU2013202354A1 (en) * 2012-06-18 2014-01-16 Speedx Pty Ltd Target detection and signal amplification
CN103013953B (zh) * 2012-10-19 2014-09-17 中国科学院成都生物研究所 一种剪切dna的dna酶
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Also Published As

Publication number Publication date
US6140055A (en) 2000-10-31
US20010001063A1 (en) 2001-05-10
IL138126A0 (en) 2001-10-31
US6365724B2 (en) 2002-04-02
US6201113B1 (en) 2001-03-13
AU3340999A (en) 1999-09-20
ATE414169T1 (de) 2008-11-15
IL164182A0 (en) 2005-12-18
EP1062367A4 (de) 2003-07-30
KR100748863B1 (ko) 2007-08-13
CN1319142A (zh) 2001-10-24
AU768627B2 (en) 2003-12-18
EP1062367B1 (de) 2008-11-12
EP1062367A1 (de) 2000-12-27
CA2321948A1 (en) 1999-09-10
JP4318856B2 (ja) 2009-08-26
CN1167811C (zh) 2004-09-22
JP2002505117A (ja) 2002-02-19
WO1999045146A1 (en) 1999-09-10
ZA991772B (en) 2000-10-12
BR9908567A (pt) 2000-12-12
KR20010041591A (ko) 2001-05-25

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