WO2002044405A3 - Verfahren und kit zum direkten nachweis von nukleotidsequenzen, aminosäuresequenzen oder antigenen - Google Patents

Verfahren und kit zum direkten nachweis von nukleotidsequenzen, aminosäuresequenzen oder antigenen Download PDF

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Publication number
WO2002044405A3
WO2002044405A3 PCT/EP2001/013980 EP0113980W WO0244405A3 WO 2002044405 A3 WO2002044405 A3 WO 2002044405A3 EP 0113980 W EP0113980 W EP 0113980W WO 0244405 A3 WO0244405 A3 WO 0244405A3
Authority
WO
WIPO (PCT)
Prior art keywords
target
dna
strand
amino acid
destabilising
Prior art date
Application number
PCT/EP2001/013980
Other languages
English (en)
French (fr)
Other versions
WO2002044405A2 (de
Inventor
Werner Lehmann
Original Assignee
Attomol Gmbh Molekulare Diagno
Werner Lehmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Attomol Gmbh Molekulare Diagno, Werner Lehmann filed Critical Attomol Gmbh Molekulare Diagno
Priority to AU2002221909A priority Critical patent/AU2002221909A1/en
Publication of WO2002044405A2 publication Critical patent/WO2002044405A2/de
Publication of WO2002044405A3 publication Critical patent/WO2002044405A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zum direkten Nachweis von Target-Nukleotidsequenzen, Target-Aminosäuresequenzen oder Target-Antigenen durch Amplifikation von DNS unter Verwendung einer DNS-Polymerase und eines DNS-doppelstrangdestabilisierenden Enzyms, wobei das Target-Molekül in situ oder in Lösung vorliegt oder direkt oder über eine Fangsonde an eine feste Phase immobilisiert wird, eine zur Strangverdrängung befähigte DNS-Polymerase, dNTPs und ein DNS-doppelstrangdestabilisierendes Enzym zugegeben werden und das entstehende Amplifikat direkt detektiert und über die Detektion das Target nachgewiesen wird oder für den Nachweis nichtamplifizierbarer Targetmoleküle zunächst eine Matrix-DNS tragende Kopplungssonde zugegeben wird und anschließend eine zur Strangverdrängung befähigte DNS-Polymerase, dNTPs und ein DNS-doppelstrangdestabilisierendes Enzym zugegeben werden und das entstehende Amplifikat direkt detektiert und über die Detektion das Target nachgewiesen wird.
PCT/EP2001/013980 2000-11-30 2001-11-30 Verfahren und kit zum direkten nachweis von nukleotidsequenzen, aminosäuresequenzen oder antigenen WO2002044405A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002221909A AU2002221909A1 (en) 2000-11-30 2001-11-30 Method and kit for the direct identification of nucleotide sequences, amino acid sequences or antigens

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10061166A DE10061166A1 (de) 2000-11-30 2000-11-30 Verfahren und Kit zum direkten Nachweis von Nukleotidsequenzen, Aminosäuresequenzen oder Antigenen
DE10061166.4 2000-11-30

Publications (2)

Publication Number Publication Date
WO2002044405A2 WO2002044405A2 (de) 2002-06-06
WO2002044405A3 true WO2002044405A3 (de) 2003-03-20

Family

ID=7666347

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/013980 WO2002044405A2 (de) 2000-11-30 2001-11-30 Verfahren und kit zum direkten nachweis von nukleotidsequenzen, aminosäuresequenzen oder antigenen

Country Status (3)

Country Link
AU (1) AU2002221909A1 (de)
DE (1) DE10061166A1 (de)
WO (1) WO2002044405A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2613101A1 (en) * 2005-07-01 2007-01-11 Bioveris Corporation Compositions and methods for detecting, amplifying, and/or isolating nucleic acids

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3929030A1 (de) * 1989-09-01 1991-03-07 Boehringer Mannheim Gmbh Verfahren zur vermehrung von nukleinsaeuren
EP0744470A1 (de) * 1995-05-22 1996-11-27 JOHNSON & JOHNSON CLINICAL DIAGNOSTICS, INC. Verfahren zur Voramplifikationsterilisierung unter Verwendung von Exonukleasen in der Polymerasekettenreaktion in der Anwesenheit von phosphothiodierten Primen
US5736365A (en) * 1993-05-05 1998-04-07 Becton, Dickinson And Company Multiplex nucleic acid amplification
US5919630A (en) * 1997-05-13 1999-07-06 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
WO2000061816A1 (en) * 1999-04-12 2000-10-19 Nanogen/Becton Dickinson Partnership Electronically mediated nucleic acid amplification in nasba

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3929030A1 (de) * 1989-09-01 1991-03-07 Boehringer Mannheim Gmbh Verfahren zur vermehrung von nukleinsaeuren
US5736365A (en) * 1993-05-05 1998-04-07 Becton, Dickinson And Company Multiplex nucleic acid amplification
EP0744470A1 (de) * 1995-05-22 1996-11-27 JOHNSON & JOHNSON CLINICAL DIAGNOSTICS, INC. Verfahren zur Voramplifikationsterilisierung unter Verwendung von Exonukleasen in der Polymerasekettenreaktion in der Anwesenheit von phosphothiodierten Primen
US5919630A (en) * 1997-05-13 1999-07-06 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
WO2000061816A1 (en) * 1999-04-12 2000-10-19 Nanogen/Becton Dickinson Partnership Electronically mediated nucleic acid amplification in nasba

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NADEAU J G ET AL: "REAL-TIME, SEQUENCE-SPECIFIC DETECTION OF NUCLEIC ACIDS DURING STRAND DISPLACEMENT AMPLIFICATION", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 276, no. 2, 15 December 1999 (1999-12-15), pages 177 - 187, XP000906307, ISSN: 0003-2697 *

Also Published As

Publication number Publication date
WO2002044405A2 (de) 2002-06-06
DE10061166A1 (de) 2002-06-06
AU2002221909A1 (en) 2002-06-11

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