WO2002024944A3 - Verfahren zum nachweis mindestens einer nukleinsäuresequenz - Google Patents

Verfahren zum nachweis mindestens einer nukleinsäuresequenz Download PDF

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Publication number
WO2002024944A3
WO2002024944A3 PCT/DE2001/003564 DE0103564W WO0224944A3 WO 2002024944 A3 WO2002024944 A3 WO 2002024944A3 DE 0103564 W DE0103564 W DE 0103564W WO 0224944 A3 WO0224944 A3 WO 0224944A3
Authority
WO
WIPO (PCT)
Prior art keywords
detecting
nucleic acid
acid sequence
primer
identification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2001/003564
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English (en)
French (fr)
Other versions
WO2002024944A2 (de
Inventor
Juergen Schuelein
Hans Kosak
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Original Assignee
November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin filed Critical November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Priority to EP01982109A priority Critical patent/EP1327004A2/de
Publication of WO2002024944A2 publication Critical patent/WO2002024944A2/de
Anticipated expiration legal-status Critical
Publication of WO2002024944A3 publication Critical patent/WO2002024944A3/de
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft einen Kit zur Durchführung eines Verfahrens enthaltend: a) an einem Träger immobilisierte Bindungssequenzen und b) Verbindungen, jeweils gebildet aus einem für die nachzuweisenden Nukleinsäuren spezifischen ersten Primern und einer für den ersten Primer spezifischen Identifizierungssequenz, wobei die Identifizierungssequenz aus einer Gruppe von Identifizierungssequenzen ausgewählt ist, welche bei vorgegebenen einheitlichen Hybridisierungsbedingungen nicht kreuzhybridisieren.
PCT/DE2001/003564 2000-09-18 2001-09-18 Verfahren zum nachweis mindestens einer nukleinsäuresequenz Ceased WO2002024944A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP01982109A EP1327004A2 (de) 2000-09-18 2001-09-18 Verfahren zum nachweis mindestens einer nukleinsäuresequenz

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10046184.0 2000-09-18
DE10046184A DE10046184A1 (de) 2000-09-18 2000-09-18 Verfahren zum Nachweis mindestens einer Nukleinsäuresequenz

Publications (2)

Publication Number Publication Date
WO2002024944A2 WO2002024944A2 (de) 2002-03-28
WO2002024944A3 true WO2002024944A3 (de) 2003-05-08

Family

ID=7656681

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/003564 Ceased WO2002024944A2 (de) 2000-09-18 2001-09-18 Verfahren zum nachweis mindestens einer nukleinsäuresequenz

Country Status (3)

Country Link
EP (1) EP1327004A2 (de)
DE (1) DE10046184A1 (de)
WO (1) WO2002024944A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10253337B4 (de) * 2002-11-14 2005-10-20 November Ag Molekulare Medizin Verfahren zum Nachweis einer Nukleinsäure
US20050026181A1 (en) * 2003-04-29 2005-02-03 Genvault Corporation Bio bar-code
FR2949120A1 (fr) * 2009-08-13 2011-02-18 Centre Nat Rech Scient Procede de detection d'un adn circularise et utilisation de ce procede pour la detection de mutations
CN102639714A (zh) * 2009-10-29 2012-08-15 日本碍子株式会社 靶核酸的检测方法
JPWO2012070618A1 (ja) 2010-11-24 2014-05-19 株式会社カネカ 増幅核酸検出方法及び検出デバイス
ES2736977T3 (es) * 2011-09-14 2020-01-09 Ngk Insulators Ltd Método para detectar ácido nucleico diana
WO2013162026A1 (ja) 2012-04-27 2013-10-31 株式会社カネカ 核酸の増幅方法、および、増幅核酸の検出方法
WO2015076356A1 (ja) * 2013-11-22 2015-05-28 株式会社カネカ 短鎖ｒｎａの検出方法
AU2018400335A1 (en) * 2018-01-05 2020-07-02 Quotient Suisse Sa Self-assembling diagnostic array platform

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0416817A2 (de) * 1989-09-06 1991-03-13 Zeneca Limited Amplifikationsverfahren
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL113519A (en) * 1990-08-03 1997-11-20 Sterling Winthrop Inc Oligonucleoside sequences of from about 6 to about 200 bases having a three atom internucleoside linkage, their preparation and pharmaceutical compositions for inhibiting gene expression containing said oligonucleosides
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US6090553A (en) * 1997-10-29 2000-07-18 Beckman Coulter, Inc. Use of uracil-DNA glycosylase in genetic analysis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0416817A2 (de) * 1989-09-06 1991-03-13 Zeneca Limited Amplifikationsverfahren
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DIEFFENBACH C W ET AL: "GENERAL CONCEPTS FOR PCR PRIMER DESIGN", PCR METHODS & APPLICATIONS, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 3, 1 December 1993 (1993-12-01), pages S30 - S37, XP000616084, ISSN: 1054-9803 *
NEWTON C R ET AL: "THE PRODUCTION OF PCR PRODUCTS WITH 5' SINGLE-STRANDED TAILS USING PRIMERS THAT INCORPORATE NOVEL PHOSPHORAMIDITE INTERMEDIATES", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 21, no. 5, 1993, pages 1155 - 1162, XP002041613, ISSN: 0305-1048 *
WHITCOMBE DAVID ET AL: "Detection of PCR products using self-probing amplicons and fluorescence.", NATURE BIOTECHNOLOGY, vol. 17, no. 8, August 1999 (1999-08-01), pages 804 - 807, XP002226672, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
WO2002024944A2 (de) 2002-03-28
EP1327004A2 (de) 2003-07-16
DE10046184A1 (de) 2002-04-04

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