WO1993004199A3 - Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids - Google Patents

Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids Download PDF

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Publication number
WO1993004199A3
WO1993004199A3 PCT/GB1992/001526 GB9201526W WO9304199A3 WO 1993004199 A3 WO1993004199 A3 WO 1993004199A3 GB 9201526 W GB9201526 W GB 9201526W WO 9304199 A3 WO9304199 A3 WO 9304199A3
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WO
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Application
Patent type
Prior art keywords
nucleic acids
methods
detecting
quantitating
producing labelled
Prior art date
Application number
PCT/GB1992/001526
Other languages
French (fr)
Other versions
WO1993004199A2 (en )
Inventor
Adrian Parton
Original Assignee
Scient Generics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

Methods for determining the presence or absence of a certain nucleic acid, for producing immobilized nucleic acids and for carrying out cyclic amplification methods like PCR or LCR using immobilized probe-primers are disclosed.
PCT/GB1992/001526 1991-08-20 1992-08-19 Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids WO1993004199A3 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
GB9117902A GB9117902D0 (en) 1991-08-20 1991-08-20 Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids
GB9117902.8 1991-08-20
GB9202962A GB9202962D0 (en) 1992-02-12 1992-02-12 Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids
GB9202962.8 1992-02-12

Publications (2)

Publication Number Publication Date
WO1993004199A2 true WO1993004199A2 (en) 1993-03-04
WO1993004199A3 true true WO1993004199A3 (en) 1993-04-15

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1992/001526 WO1993004199A3 (en) 1991-08-20 1992-08-19 Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids

Country Status (1)

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WO (1) WO1993004199A3 (en)

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US6277975B1 (en) 1992-10-23 2001-08-21 Genetics Institute, Inc. Fusions of P-selectin ligand protein and polynucleotides encoding same
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US6001571A (en) 1995-11-30 1999-12-14 Mandecki; Wlodek Multiplex assay for nucleic acids employing transponders
WO1997019958A1 (en) 1995-11-30 1997-06-05 Wlodek Mandecki Screening of drugs from chemical combinatorial libraries employing transponders
US5641634A (en) 1995-11-30 1997-06-24 Mandecki; Wlodek Electronically-indexed solid-phase assay for biomolecules
US6051377A (en) * 1995-11-30 2000-04-18 Pharmaseq, Inc. Multiplex assay for nucleic acids employing transponders
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US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
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US5981166A (en) * 1997-04-23 1999-11-09 Pharmaseq, Inc. Screening of soluble chemical compounds for their pharmacological properties utilizing transponders
WO1999022023A3 (en) * 1997-10-29 1999-09-16 Mira Diagnostica Gmbh Method for identifying micro-organisms
US20020081583A1 (en) * 1998-01-08 2002-06-27 Satoshi Abe Probes for detecting of target nucleic acid, method of detecting target nucleic acid, and solid phase for detecting target acid and process for producing the same
DE69928683D1 (en) 1998-09-30 2006-01-05 Solexa Ltd A method for amplifying and sequencing of nucleic acids
BE1012275A3 (en) * 1998-11-12 2000-08-01 Univ Bruxelles Covalent oligonucleotide fixation to nylon surface
GB9904991D0 (en) * 1999-03-05 1999-04-28 Univ Nottingham Genetic screening
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EP1255860A2 (en) * 1999-12-29 2002-11-13 Mergen Ltd. Methods for amplifying and detecting multiple polynucleotides on a solid phase support
JP5508654B2 (en) 2000-04-14 2014-06-04 コーネル・リサーチ・ファンデーション・インコーポレイテッドCornell Research Foundation, Incorporated Design method for localization possible array for the detection of differences in nucleic acid sequences using ligase detection reaction
EP1207209A3 (en) * 2000-11-09 2002-09-11 Agilent Technologies, Inc. (a Delaware corporation) Methods using arrays for detection of single nucleotide polymorphisms
CA2442939A1 (en) 2001-04-02 2002-10-17 Point-2-Point Genomics Ltd. Polynucleotide analysis using combinatorial pcr
US7790418B2 (en) 2000-12-08 2010-09-07 Illumina Cambridge Limited Isothermal amplification of nucleic acids on a solid support
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GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
US8822670B2 (en) 2005-08-19 2014-09-02 Sumitomo Bakelite Company, Ltd. Method of synthesizing cDNA and method of synthesizing RNA chains, and nucleotide-immobilized carrier
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
CA2641851A1 (en) 2006-02-08 2007-08-16 Eric Hans Vermaas Method for sequencing a polynucleotide template
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
EP2121983A2 (en) 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
FI20075124A0 (en) * 2007-02-21 2007-02-21 Valtion Teknillinen The method and test kit for the detection of nucleotide variations
US8728764B2 (en) 2008-10-02 2014-05-20 Illumina Cambridge Limited Nucleic acid sample enrichment for sequencing applications
EP2669387B1 (en) 2009-08-25 2016-07-20 Illumina, Inc. Methods for selecting and amplifying polynucleotides
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
GB201301857D0 (en) * 2013-02-01 2013-03-20 Selvi Ozan Method

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WO1989011546A1 (en) * 1988-05-24 1989-11-30 Gunnar Paulsen Dna-analysis method involving gene amplification and magnetic particles
WO1990006042A2 (en) * 1988-11-21 1990-06-14 Dynal As Detection and quantitative determination of rna and dna
EP0374665A2 (en) * 1988-12-23 1990-06-27 Miles Inc. Assay of sequences using amplified genes
WO1990011369A1 (en) * 1989-03-22 1990-10-04 Cemu Bioteknik Ab Solid phase diagnosis of medical conditions
WO1991000868A1 (en) * 1989-07-07 1991-01-24 National Research Development Corporation Immobilised polynucleotides

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988001302A1 (en) * 1986-08-11 1988-02-25 Siska Diagnostics, Inc. Nucleic acid probe assay methods and compositions
WO1989011546A1 (en) * 1988-05-24 1989-11-30 Gunnar Paulsen Dna-analysis method involving gene amplification and magnetic particles
WO1990006042A2 (en) * 1988-11-21 1990-06-14 Dynal As Detection and quantitative determination of rna and dna
EP0374665A2 (en) * 1988-12-23 1990-06-27 Miles Inc. Assay of sequences using amplified genes
WO1990011369A1 (en) * 1989-03-22 1990-10-04 Cemu Bioteknik Ab Solid phase diagnosis of medical conditions
WO1991000868A1 (en) * 1989-07-07 1991-01-24 National Research Development Corporation Immobilised polynucleotides

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