WO2002012553A3 - Verfahren zum nachweis von mutationen in nucleotidsequenzen - Google Patents

Verfahren zum nachweis von mutationen in nucleotidsequenzen Download PDF

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Publication number
WO2002012553A3
WO2002012553A3 PCT/EP2001/008127 EP0108127W WO0212553A3 WO 2002012553 A3 WO2002012553 A3 WO 2002012553A3 EP 0108127 W EP0108127 W EP 0108127W WO 0212553 A3 WO0212553 A3 WO 0212553A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleotide sequences
stranded
detecting mutations
fixating
mutated
Prior art date
Application number
PCT/EP2001/008127
Other languages
English (en)
French (fr)
Other versions
WO2002012553A2 (de
Inventor
Andreas Kappel
Thomas Polakowski
Marc Pignot
Norbert Windhab
Heike Behrensdorf
Jochen Muth
Original Assignee
Nanogen Recognomics Gmbh
Andreas Kappel
Thomas Polakowski
Marc Pignot
Norbert Windhab
Heike Behrensdorf
Jochen Muth
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanogen Recognomics Gmbh, Andreas Kappel, Thomas Polakowski, Marc Pignot, Norbert Windhab, Heike Behrensdorf, Jochen Muth filed Critical Nanogen Recognomics Gmbh
Priority to AU2001270635A priority Critical patent/AU2001270635A1/en
Priority to CA002417861A priority patent/CA2417861A1/en
Priority to IL15427001A priority patent/IL154270A0/xx
Priority to US10/343,859 priority patent/US20040110161A1/en
Priority to EP01949494A priority patent/EP1307589A2/de
Priority to JP2002517837A priority patent/JP2004520010A/ja
Publication of WO2002012553A2 publication Critical patent/WO2002012553A2/de
Publication of WO2002012553A3 publication Critical patent/WO2002012553A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Verfahren, mit dem Mutationen parallel in verschiedenen Nucleotidsequenzen nachgewiesen werden können, wobei das Verfahren daneben die Bestimmung der Transkriptionsrate von mutierten und nicht mutierten Nucleotidsequenzen erlaubt. Das Verfahren umfasst die Verfahrensschritte der Hybridisierung einzelsträngiger Proben-Nucleotidsequenzen mit einzelsträngigen Referenz-Nucleotidsequenzen, Fixierung von einzelsträngigen Referenz-Nucleotidsequenzen oder einzelsträngigen Proben-Nucleotidsequenzen vor oder während der Hybridisierung oder von Heteroduplices aus Referenz- und Proben-Nucleotidsequenzen nach oder während der Hybridisierung auf einer elektronisch adressierbaren Oberfläche, Inkubation mit einem Heteroduplex-Fehlpaarungen erkennenden Substrat und Detektion der Substratbindungen.
PCT/EP2001/008127 2000-08-04 2001-07-13 Verfahren zum nachweis von mutationen in nucleotidsequenzen WO2002012553A2 (de)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU2001270635A AU2001270635A1 (en) 2000-08-04 2001-07-13 Method for detecting mutations in nucleotide sequences
CA002417861A CA2417861A1 (en) 2000-08-04 2001-07-13 Method for detecting mutations in nucleotide sequences
IL15427001A IL154270A0 (en) 2000-08-04 2001-07-13 Method for detecting mutations in nucleotide sequences
US10/343,859 US20040110161A1 (en) 2000-08-04 2001-07-13 Method for detecting mutations in nucleotide sequences
EP01949494A EP1307589A2 (de) 2000-08-04 2001-07-13 Verfahren zum nachweis von mutationen in nucleotidsequenzen
JP2002517837A JP2004520010A (ja) 2000-08-04 2001-07-13 ヌクレオチド配列中の突然変異を検出するための方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10038237.1 2000-08-04
DE10038237A DE10038237A1 (de) 2000-08-04 2000-08-04 Verfahren zum Nachweis von Mutationen in Nucleotidsequenzen

Publications (2)

Publication Number Publication Date
WO2002012553A2 WO2002012553A2 (de) 2002-02-14
WO2002012553A3 true WO2002012553A3 (de) 2003-01-30

Family

ID=7651415

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/008127 WO2002012553A2 (de) 2000-08-04 2001-07-13 Verfahren zum nachweis von mutationen in nucleotidsequenzen

Country Status (8)

Country Link
US (1) US20040110161A1 (de)
EP (1) EP1307589A2 (de)
JP (1) JP2004520010A (de)
AU (1) AU2001270635A1 (de)
CA (1) CA2417861A1 (de)
DE (1) DE10038237A1 (de)
IL (1) IL154270A0 (de)
WO (1) WO2002012553A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003070977A2 (en) * 2002-02-21 2003-08-28 Nanogen Recognomics Gmbh Method for detecting single nucleotide polymorphisms
WO2004061100A1 (ja) * 2002-12-10 2004-07-22 Olympus Corporation 核酸の変異解析方法および遺伝子の発現解析方法
WO2005001122A2 (en) * 2003-06-27 2005-01-06 Adnavance Technologies, Inc. Electrochemical detection of dna binding
GB0624071D0 (en) * 2006-12-01 2007-01-10 Bioline Res Ltd Purification methods and materials
US8168120B1 (en) 2007-03-06 2012-05-01 The Research Foundation Of State University Of New York Reliable switch that is triggered by the detection of a specific gas or substance
GB0718957D0 (en) * 2007-09-28 2007-11-07 Ge Healthcare Ltd Optical imaging agents
GB0718967D0 (en) * 2007-09-28 2007-11-07 Ge Healthcare Ltd Peptide imaging agents
JO3076B1 (ar) 2007-10-17 2017-03-15 Janssen Alzheimer Immunotherap نظم العلاج المناعي المعتمد على حالة apoe
JP5300335B2 (ja) * 2008-06-06 2013-09-25 株式会社日清製粉グループ本社 ミスマッチ塩基対含有核酸断片の高効率調製方法

Citations (7)

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Publication number Priority date Publication date Assignee Title
US5750335A (en) * 1992-04-24 1998-05-12 Massachusetts Institute Of Technology Screening for genetic variation
US5849486A (en) * 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
WO1999006591A1 (fr) * 1997-07-31 1999-02-11 The Institute Of Physical And Chemical Research Procede de detection de mutation dans une sequence de bases
WO1999039003A1 (en) * 1998-01-30 1999-08-05 Genzyme Corporation Method for detecting and identifying mutations
US6017696A (en) * 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US6051380A (en) * 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US6068818A (en) * 1993-11-01 2000-05-30 Nanogen, Inc. Multicomponent devices for molecular biological analysis and diagnostics

Family Cites Families (3)

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Publication number Priority date Publication date Assignee Title
ATE151114T1 (de) * 1991-07-19 1997-04-15 Upstate Biotech Inc Verfahren zum nachweis von mutationen
US6027877A (en) * 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
AU4833899A (en) * 1998-06-24 2000-01-10 Therasense, Inc. Multi-sensor array for electrochemical recognition of nucleotide sequences and methods

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5750335A (en) * 1992-04-24 1998-05-12 Massachusetts Institute Of Technology Screening for genetic variation
US5849486A (en) * 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
US6017696A (en) * 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US6051380A (en) * 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US6068818A (en) * 1993-11-01 2000-05-30 Nanogen, Inc. Multicomponent devices for molecular biological analysis and diagnostics
WO1999006591A1 (fr) * 1997-07-31 1999-02-11 The Institute Of Physical And Chemical Research Procede de detection de mutation dans une sequence de bases
EP1041160A1 (de) * 1997-07-31 2000-10-04 The Institute Of Physical & Chemical Research Verfahren zur detektion einer mutation innerhalb einer basensequenz
WO1999039003A1 (en) * 1998-01-30 1999-08-05 Genzyme Corporation Method for detecting and identifying mutations

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
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GESCHWIND DANIEL H ET AL: "A biotinylated MutS fusion protein and its use in a rapid mutation screening technique.", GENETIC ANALYSIS BIOMOLECULAR ENGINEERING, vol. 13, no. 4, 1996, pages 105 - 111, XP002200124 *
GOTOH M ET AL: "Rapid method for detection of point mutations using mismatch binding protein (MutS) and an optical biosensor", GENETIC ANALYSIS: BIOMOLECULAR ENGINEERING, ELSEVIER SCIENCE PUBLISHING, US, vol. 14, no. 2, 1 July 1997 (1997-07-01), pages 47 - 50, XP004126266, ISSN: 1050-3862 *
GRUBER H J ET AL: "Preparation of thiol-reactive Cy5 derivatives from commercial Cy5 succinimidyl ester.", BIOCONJUGATE CHEMISTRY. UNITED STATES 2000 MAR-APR, vol. 11, no. 2, March 2000 (2000-03-01), pages 161 - 166, XP002212302, ISSN: 1043-1802 *
MUJUMDAR S R ET AL: "Cyanine-labeling reagents: sulfobenzindocyanine succinimidyl esters.", BIOCONJUGATE CHEMISTRY. UNITED STATES 1996 MAY-JUN, vol. 7, no. 3, May 1996 (1996-05-01), pages 356 - 362, XP002212303, ISSN: 1043-1802 *
SACHADYN P ET AL: "One tube mutation detection using sensitive fluorescent dyeing of MutS protected DNA.", NUCLEIC ACIDS RESEARCH. ENGLAND 15 APR 2000, vol. 28, no. 8, 15 April 2000 (2000-04-15), pages E36, XP002200123, ISSN: 1362-4962 *
TAYLOR G R ET AL: "Enzymatic methods for mutation scanning", GENETIC ANALYSIS: BIOMOLECULAR ENGINEERING, ELSEVIER SCIENCE PUBLISHING, US, vol. 14, no. 5-6, February 1999 (1999-02-01), pages 181 - 186, XP004158702, ISSN: 1050-3862 *

Also Published As

Publication number Publication date
AU2001270635A1 (en) 2002-02-18
CA2417861A1 (en) 2003-02-03
JP2004520010A (ja) 2004-07-08
EP1307589A2 (de) 2003-05-07
DE10038237A1 (de) 2002-02-14
WO2002012553A2 (de) 2002-02-14
IL154270A0 (en) 2003-09-17
US20040110161A1 (en) 2004-06-10

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