MX9706501A - Deteccion de biomoleculas. - Google Patents

Deteccion de biomoleculas.

Info

Publication number
MX9706501A
MX9706501A MX9706501A MX9706501A MX9706501A MX 9706501 A MX9706501 A MX 9706501A MX 9706501 A MX9706501 A MX 9706501A MX 9706501 A MX9706501 A MX 9706501A MX 9706501 A MX9706501 A MX 9706501A
Authority
MX
Mexico
Prior art keywords
detection
ribozyme
biomolecules
active ribozyme
catalytically active
Prior art date
Application number
MX9706501A
Other languages
English (en)
Other versions
MXPA97006501A (es
Inventor
Nathan Asher
Yaron Tikochinski
Guido Krupp
Jacob Grinberg
Adam Friedmann
Original Assignee
Intelligene Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from IL11279995A external-priority patent/IL112799A/xx
Priority claimed from IL11572295A external-priority patent/IL115722A0/xx
Application filed by Intelligene Ltd filed Critical Intelligene Ltd
Publication of MXPA97006501A publication Critical patent/MXPA97006501A/es
Publication of MX9706501A publication Critical patent/MX9706501A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La presente invencion se relaciona con un método para la deteccion de la presencia de un ribozima catalíticamente activa en un medio. La deteccion de la ribozima catalíticamente activa puede ser un objetivo por sí mismo o la ribozima puede servir como una molécula de la informacion para la presencia de otras biomoléculas en muestras ensayadas. La deteccion se lleva a cabo en un sistema catalítico, en el que la presencia de la ribozima activa sirve para producir la ribozima activa en una forma amplificadora de la retroalimentacion positiva.
MX9706501A 1995-02-27 1996-02-27 Deteccion de biomoleculas. MX9706501A (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
IL11279995A IL112799A (en) 1995-02-27 1995-02-27 Detection of biomolecules
IL112799 1995-02-27
IL11572295A IL115722A0 (en) 1995-03-24 1995-10-23 Patient handling system for diagnostic imaging applications
IL115722 1995-10-26
PCT/US1996/002380 WO1996027026A1 (en) 1995-02-27 1996-02-27 Detection of biomolecules

Publications (2)

Publication Number Publication Date
MXPA97006501A MXPA97006501A (es) 1998-02-01
MX9706501A true MX9706501A (es) 1998-02-28

Family

ID=26322996

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9706501A MX9706501A (es) 1995-02-27 1996-02-27 Deteccion de biomoleculas.

Country Status (5)

Country Link
EP (1) EP0822992A4 (es)
JP (1) JPH11500917A (es)
AU (1) AU697317B2 (es)
MX (1) MX9706501A (es)
WO (1) WO1996027026A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE253128T1 (de) * 1996-08-26 2003-11-15 Genetico Ltd Katalytische nukleinsäuren und deren medizinische verwendung
IL120466A0 (en) * 1997-03-17 1997-07-13 Intelligene Ltd In vitro evolution of oligonucleotides
JP4318856B2 (ja) * 1998-03-05 2009-08-26 ジョンソン・アンド・ジョンソン・リサーチ・ピー・ティー・ワイ・リミテッド チモーゲン性核酸検出方法、および関連分子およびキット
DE19811618C1 (de) * 1998-03-17 2000-08-31 Andreas Jenne Ribozym codierende DNA und ein Oligonucleotidsubstrat enthaltende Zusammensetzung und Verfahren zur Messung von Transkriptionsraten
DE19915141C2 (de) * 1999-03-26 2002-11-21 Artus Ges Fuer Molekularbiolog Detektion von Nucleinsäure-Amplifikaten
IL130606A0 (en) * 1999-06-23 2000-06-01 Intelligene Ltd An array of nucleic acid sequence
US7125660B2 (en) 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
AU2002355571A1 (en) * 2001-08-09 2003-02-24 Archemix Corporation Nucleic acid sensor molecules and methods of using same
WO2006086499A2 (en) * 2005-02-08 2006-08-17 Applera Corporation Nucleozymes and methods of use
BRPI0616466B1 (pt) 2005-10-07 2021-01-26 Johnson & Johnson Research Pty Limited composição de enzimas de ácido nucleico multicomponentes (mnazima), métodos para detectar a presença de pelo menos um facilitador de combinação, um alvo e uma variante de sequência de ácidos nucléicos, métodos para fabricar uma pluraridade de mnazima, testar sequências de núcleo catalítico parcial e identificar suas posições, bem como uso de pelo menos um oligonucleotídeo
WO2008040095A1 (en) 2006-10-06 2008-04-10 Johnson & Johnson Research Pty Limited Molecular switches and methods for their use
EP2142673B1 (en) 2007-04-05 2013-05-22 Johnson & Johnson Research Pty Limited Nucleic acid enzymes and complexes and methods for their use
CN106232832B (zh) 2013-12-06 2022-04-05 哈佛大学校长及研究员协会 基于纸的合成基因网络
JP7486951B2 (ja) * 2017-04-11 2024-05-20 スピーディックス プロプライアタリー リミティド 検出カスケード

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1195442A3 (en) * 1992-12-04 2002-07-31 Innovir Laboratories, Inc. Ribozyme amplified diagnostics
JPH08505531A (ja) * 1993-01-15 1996-06-18 ザ パブリック ヘルス リサーチ インスティチュート オブ ザ シティー オブ ニューヨーク インク Ｒｎａバイナリ・プローブとリボザイムリガーゼを用いたｒｎａ検定法
IL105894A (en) * 1993-06-03 1997-02-18 Asher Nathan Method for the detection of nucleic acid sequence by using a detection ensemble and an amplification ensemble

Also Published As

Publication number Publication date
JPH11500917A (ja) 1999-01-26
AU697317B2 (en) 1998-10-01
AU5416596A (en) 1996-09-18
WO1996027026A1 (en) 1996-09-06
EP0822992A4 (en) 2000-08-09
EP0822992A1 (en) 1998-02-11

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