EP0822992A4 - Detection of biomolecules - Google Patents

Detection of biomolecules

Info

Publication number
EP0822992A4
EP0822992A4 EP96911209A EP96911209A EP0822992A4 EP 0822992 A4 EP0822992 A4 EP 0822992A4 EP 96911209 A EP96911209 A EP 96911209A EP 96911209 A EP96911209 A EP 96911209A EP 0822992 A4 EP0822992 A4 EP 0822992A4
Authority
EP
European Patent Office
Prior art keywords
detection
ribozyme
biomolecules
active ribozyme
catalytically active
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP96911209A
Other languages
German (de)
French (fr)
Other versions
EP0822992A1 (en
Inventor
Nathan Asher
Yaron Tikochinski
Guido Krupp
Jacob Grinberg
Adam Friedmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Intelligene Ltd
Original Assignee
Intelligene Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from IL11279995A external-priority patent/IL112799A/en
Priority claimed from IL11572295A external-priority patent/IL115722A0/en
Application filed by Intelligene Ltd filed Critical Intelligene Ltd
Publication of EP0822992A1 publication Critical patent/EP0822992A1/en
Publication of EP0822992A4 publication Critical patent/EP0822992A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention concerns a method for detecting the presence of a catalytically active ribozyme in a medium. The detection of the catalytically active ribozyme may be a goal by itself, or the ribozyme may serve as a reporter for the presence of other biomolecules in an assayed sample. The detection is carried out in a catalytic system wherein the presence of the active ribozyme serves to produce other active ribozyme in a positive-feedback amplificatory manner.
EP96911209A 1995-02-27 1996-02-27 Detection of biomolecules Withdrawn EP0822992A4 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
IL11279995A IL112799A (en) 1995-02-27 1995-02-27 Detection of biomolecules
IL11279995 1995-02-27
IL11572295A IL115722A0 (en) 1995-03-24 1995-10-23 Patient handling system for diagnostic imaging applications
IL11572295 1995-10-26
PCT/US1996/002380 WO1996027026A1 (en) 1995-02-27 1996-02-27 Detection of biomolecules

Publications (2)

Publication Number Publication Date
EP0822992A1 EP0822992A1 (en) 1998-02-11
EP0822992A4 true EP0822992A4 (en) 2000-08-09

Family

ID=26322996

Family Applications (1)

Application Number Title Priority Date Filing Date
EP96911209A Withdrawn EP0822992A4 (en) 1995-02-27 1996-02-27 Detection of biomolecules

Country Status (5)

Country Link
EP (1) EP0822992A4 (en)
JP (1) JPH11500917A (en)
AU (1) AU697317B2 (en)
MX (1) MX9706501A (en)
WO (1) WO1996027026A1 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE253128T1 (en) 1996-08-26 2003-11-15 Genetico Ltd CATALYTIC NUCLEIC ACIDS AND THEIR MEDICAL USE
IL120466A0 (en) * 1997-03-17 1997-07-13 Intelligene Ltd In vitro evolution of oligonucleotides
KR100748863B1 (en) * 1998-03-05 2007-08-13 존슨 앤드 존슨 리서치 피티와이 리미티드 Zymogenic nucleic acid detection methods, and related molecules and kits
DE19811618C1 (en) * 1998-03-17 2000-08-31 Andreas Jenne DNA encoding ribozyme and an oligonucleotide substrate-containing composition and method for measuring transcription rates
DE19915141C2 (en) * 1999-03-26 2002-11-21 Artus Ges Fuer Molekularbiolog Detection of nucleic acid amplificates
IL130606A0 (en) * 1999-06-23 2000-06-01 Intelligene Ltd An array of nucleic acid sequence
US7125660B2 (en) 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
AU2002355571A1 (en) * 2001-08-09 2003-02-24 Archemix Corporation Nucleic acid sensor molecules and methods of using same
WO2006086499A2 (en) * 2005-02-08 2006-08-17 Applera Corporation Nucleozymes and methods of use
EP2385141B1 (en) 2005-10-07 2013-08-07 SpeeDx Pty Ltd Multicomponent nucleic acid enzymes and methods for their use
ES2520023T3 (en) 2006-10-06 2014-11-11 Speedx Pty Ltd Molecular switches and methods for use
ES2425066T3 (en) 2007-04-05 2013-10-11 Speedx Pty Ltd Enzymes and nucleic acid complexes and methods for use
US20160312312A1 (en) 2013-12-06 2016-10-27 President And Fellows Of Harvard College Paper-based synthetic gene networks
JP7486951B2 (en) * 2017-04-11 2024-05-20 スピーディックス プロプライアタリー リミティド Detection Cascade

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994016105A1 (en) * 1993-01-15 1994-07-21 The Public Health Research Institute Of The City Of New York, Inc. Rna assays using rna binary probes and ribozyme ligase

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE217347T1 (en) * 1992-12-04 2002-05-15 Univ Yale DIAGNOSIS USING SIGNAL AMPLIFICATION BY A RIBOZYME
IL105894A (en) * 1993-06-03 1997-02-18 Asher Nathan Method for the detection of nucleic acid sequence by using a detection ensemble and an amplification ensemble

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994016105A1 (en) * 1993-01-15 1994-07-21 The Public Health Research Institute Of The City Of New York, Inc. Rna assays using rna binary probes and ribozyme ligase

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO9627026A1 *

Also Published As

Publication number Publication date
EP0822992A1 (en) 1998-02-11
AU697317B2 (en) 1998-10-01
AU5416596A (en) 1996-09-18
JPH11500917A (en) 1999-01-26
MX9706501A (en) 1998-02-28
WO1996027026A1 (en) 1996-09-06

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