AU768627B2 - Zymogenic nucleic acid detection methods, and related molecules and kits - Google Patents
Zymogenic nucleic acid detection methods, and related molecules and kits Download PDFInfo
- Publication number
- AU768627B2 AU768627B2 AU33409/99A AU3340999A AU768627B2 AU 768627 B2 AU768627 B2 AU 768627B2 AU 33409/99 A AU33409/99 A AU 33409/99A AU 3340999 A AU3340999 A AU 3340999A AU 768627 B2 AU768627 B2 AU 768627B2
- Authority
- AU
- Australia
- Prior art keywords
- nucleic acid
- primer
- target
- zymogene
- molecule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 236
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 170
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 170
- 238000001514 detection method Methods 0.000 title description 11
- 230000003462 zymogenic effect Effects 0.000 title description 4
- 230000003197 catalytic effect Effects 0.000 claims abstract description 106
- 238000000034 method Methods 0.000 claims abstract description 83
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 53
- 230000000692 anti-sense effect Effects 0.000 claims abstract description 28
- 239000013615 primer Substances 0.000 claims description 162
- 108020004414 DNA Proteins 0.000 claims description 100
- 230000003321 amplification Effects 0.000 claims description 90
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 90
- 108091027757 Deoxyribozyme Proteins 0.000 claims description 63
- 230000000694 effects Effects 0.000 claims description 59
- 102000053602 DNA Human genes 0.000 claims description 45
- 239000000758 substrate Substances 0.000 claims description 32
- 238000003776 cleavage reaction Methods 0.000 claims description 26
- 230000000295 complement effect Effects 0.000 claims description 26
- 230000007017 scission Effects 0.000 claims description 25
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 19
- 239000000203 mixture Substances 0.000 claims description 17
- 230000000977 initiatory effect Effects 0.000 claims description 16
- 108090000994 Catalytic RNA Proteins 0.000 claims description 12
- 102000053642 Catalytic RNA Human genes 0.000 claims description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims description 12
- 108091092562 ribozyme Proteins 0.000 claims description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 10
- 208000035475 disorder Diseases 0.000 claims description 9
- 241000282414 Homo sapiens Species 0.000 claims description 7
- 230000015572 biosynthetic process Effects 0.000 claims description 6
- 238000012986 modification Methods 0.000 claims description 6
- 230000004048 modification Effects 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- 108020001019 DNA Primers Proteins 0.000 claims description 5
- 239000003155 DNA primer Substances 0.000 claims description 5
- KDCGOANMDULRCW-UHFFFAOYSA-N Purine Natural products N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 claims description 5
- 241000700605 Viruses Species 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 5
- 241001465754 Metazoa Species 0.000 claims description 4
- 206010028980 Neoplasm Diseases 0.000 claims description 4
- 201000011510 cancer Diseases 0.000 claims description 4
- 150000002148 esters Chemical class 0.000 claims description 4
- 239000002773 nucleotide Substances 0.000 claims description 4
- 125000003729 nucleotide group Chemical group 0.000 claims description 4
- 125000002652 ribonucleotide group Chemical group 0.000 claims description 4
- 238000005096 rolling process Methods 0.000 claims description 4
- 239000002689 soil Substances 0.000 claims description 4
- 208000030507 AIDS Diseases 0.000 claims description 3
- 201000003883 Cystic fibrosis Diseases 0.000 claims description 3
- 208000005176 Hepatitis C Diseases 0.000 claims description 3
- 241000204031 Mycoplasma Species 0.000 claims description 3
- 241000288906 Primates Species 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- CREMABGTGYGIQB-UHFFFAOYSA-N carbon carbon Chemical compound C.C CREMABGTGYGIQB-UHFFFAOYSA-N 0.000 claims description 3
- 239000011203 carbon fibre reinforced carbon Substances 0.000 claims description 3
- 208000034737 hemoglobinopathy Diseases 0.000 claims description 3
- 238000006263 metalation reaction Methods 0.000 claims description 3
- 108700042226 ras Genes Proteins 0.000 claims description 3
- 201000008827 tuberculosis Diseases 0.000 claims description 3
- 241000700199 Cavia porcellus Species 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 claims description 2
- 241000699666 Mus <mouse, genus> Species 0.000 claims description 2
- 241000282341 Mustela putorius furo Species 0.000 claims description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 2
- 241000009328 Perro Species 0.000 claims description 2
- 241000700159 Rattus Species 0.000 claims description 2
- 238000010504 bond cleavage reaction Methods 0.000 claims description 2
- 238000006664 bond formation reaction Methods 0.000 claims description 2
- 150000004032 porphyrins Chemical class 0.000 claims description 2
- 238000011160 research Methods 0.000 claims description 2
- LRDIEHDJWYRVPT-UHFFFAOYSA-N 4-amino-5-hydroxynaphthalene-1-sulfonic acid Chemical compound C1=CC(O)=C2C(N)=CC=C(S(O)(=O)=O)C2=C1 LRDIEHDJWYRVPT-UHFFFAOYSA-N 0.000 claims 1
- 208000026350 Inborn Genetic disease Diseases 0.000 claims 1
- 208000016361 genetic disease Diseases 0.000 claims 1
- 239000003643 water by type Substances 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 description 45
- 239000000523 sample Substances 0.000 description 44
- 108091093088 Amplicon Proteins 0.000 description 22
- 101710113436 GTPase KRas Proteins 0.000 description 21
- 108091034117 Oligonucleotide Proteins 0.000 description 10
- 108020004705 Codon Proteins 0.000 description 9
- 108010006785 Taq Polymerase Proteins 0.000 description 9
- 239000000499 gel Substances 0.000 description 8
- 239000012634 fragment Substances 0.000 description 6
- 101000584612 Homo sapiens GTPase KRas Proteins 0.000 description 5
- 238000012408 PCR amplification Methods 0.000 description 5
- 238000003556 assay Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000013642 negative control Substances 0.000 description 4
- 101150105104 Kras gene Proteins 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 3
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 3
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 3
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 3
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- 210000004881 tumor cell Anatomy 0.000 description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 2
- 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229920006317 cationic polymer Polymers 0.000 description 2
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000002060 fluorescence correlation spectroscopy Methods 0.000 description 2
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 238000000329 molecular dynamics simulation Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000002205 phenol-chloroform extraction Methods 0.000 description 2
- UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical group [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000005180 public health Effects 0.000 description 2
- 125000000561 purinyl group Chemical group N1=C(N=C2N=CNC2=C1)* 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 1
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000700721 Hepatitis B virus Species 0.000 description 1
- 101100086477 Homo sapiens KRAS gene Proteins 0.000 description 1
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 101710141795 Ribonuclease inhibitor Proteins 0.000 description 1
- 229940122208 Ribonuclease inhibitor Drugs 0.000 description 1
- 102100037968 Ribonuclease inhibitor Human genes 0.000 description 1
- 108091006629 SLC13A2 Proteins 0.000 description 1
- 241000251131 Sphyrna Species 0.000 description 1
- 241001441622 Ulua Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 229960001456 adenosine triphosphate Drugs 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000013024 dilution buffer Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 125000001153 fluoro group Chemical group F* 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000012678 infectious agent Substances 0.000 description 1
- 208000018337 inherited hemoglobinopathy Diseases 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- -1 nucleic acids porphyrin Chemical class 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 238000003322 phosphorimaging Methods 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- NLJUOWMZPAVXHU-UHFFFAOYSA-N prop-2-enamide;urea Chemical compound NC(N)=O.NC(=O)C=C NLJUOWMZPAVXHU-UHFFFAOYSA-N 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 239000003161 ribonuclease inhibitor Substances 0.000 description 1
- 125000000548 ribosyl group Chemical group C1([C@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical group C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- NLIVDORGVGAOOJ-MAHBNPEESA-M xylene cyanol Chemical compound [Na+].C1=C(C)C(NCC)=CC=C1C(\C=1C(=CC(OS([O-])=O)=CC=1)OS([O-])=O)=C\1C=C(C)\C(=[NH+]/CC)\C=C/1 NLIVDORGVGAOOJ-MAHBNPEESA-M 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2004201097A AU2004201097A1 (en) | 1998-03-05 | 2004-03-16 | Zymogenic nucleic acid detection methods, and related molecules and kits |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7689998P | 1998-03-05 | 1998-03-05 | |
| US60/076899 | 1998-03-05 | ||
| PCT/IB1999/000754 WO1999045146A1 (en) | 1998-03-05 | 1999-02-22 | Zymogenic nucleic acid detection methods, and related molecules and kits |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2004201097A Division AU2004201097A1 (en) | 1998-03-05 | 2004-03-16 | Zymogenic nucleic acid detection methods, and related molecules and kits |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU3340999A AU3340999A (en) | 1999-09-20 |
| AU768627B2 true AU768627B2 (en) | 2003-12-18 |
Family
ID=22134859
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU33409/99A Ceased AU768627B2 (en) | 1998-03-05 | 1999-02-22 | Zymogenic nucleic acid detection methods, and related molecules and kits |
Country Status (13)
| Country | Link |
|---|---|
| US (3) | US6140055A (enExample) |
| EP (1) | EP1062367B1 (enExample) |
| JP (1) | JP4318856B2 (enExample) |
| KR (1) | KR100748863B1 (enExample) |
| CN (1) | CN1167811C (enExample) |
| AT (1) | ATE414169T1 (enExample) |
| AU (1) | AU768627B2 (enExample) |
| BR (1) | BR9908567A (enExample) |
| CA (1) | CA2321948A1 (enExample) |
| DE (1) | DE69939896D1 (enExample) |
| IL (2) | IL138126A0 (enExample) |
| WO (1) | WO1999045146A1 (enExample) |
| ZA (1) | ZA991772B (enExample) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994003624A1 (en) * | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
| US6261808B1 (en) * | 1992-08-04 | 2001-07-17 | Replicon, Inc. | Amplification of nucleic acid molecules via circular replicons |
| CA2321948A1 (en) | 1998-03-05 | 1999-09-10 | Johnson & Johnson Research Pty. Limited | Zymogenic nucleic acid detection methods, and related molecules and kits |
| DE19811618C1 (de) * | 1998-03-17 | 2000-08-31 | Andreas Jenne | Ribozym codierende DNA und ein Oligonucleotidsubstrat enthaltende Zusammensetzung und Verfahren zur Messung von Transkriptionsraten |
| AUPQ495700A0 (en) * | 2000-01-05 | 2000-02-03 | Johnson & Johnson Research Pty. Limited | Method for concurrent amplification and real time detection of polymorphic nucleic acid sequences |
| US7205129B1 (en) * | 2000-02-28 | 2007-04-17 | Qiagen Gmbh | Method for reducing artifacts in nucleic acid amplification |
| AU2001268481A1 (en) * | 2000-06-15 | 2001-12-24 | Board Of Regents, The University Of Texas System | Regulatable, catalytically active nucleic acids |
| US7125660B2 (en) | 2000-09-13 | 2006-10-24 | Archemix Corp. | Nucleic acid sensor molecules and methods of using same |
| WO2003014375A2 (en) * | 2001-08-09 | 2003-02-20 | Archemix Corporation | Nucleic acid sensor molecules and methods of using same |
| US7297485B2 (en) * | 2001-10-15 | 2007-11-20 | Qiagen Gmbh | Method for nucleic acid amplification that results in low amplification bias |
| US6617137B2 (en) * | 2001-10-15 | 2003-09-09 | Molecular Staging Inc. | Method of amplifying whole genomes without subjecting the genome to denaturing conditions |
| US7955795B2 (en) * | 2003-06-06 | 2011-06-07 | Qiagen Gmbh | Method of whole genome amplification with reduced artifact production |
| US9487823B2 (en) | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
| US8043834B2 (en) | 2003-03-31 | 2011-10-25 | Qiagen Gmbh | Universal reagents for rolling circle amplification and methods of use |
| KR100890885B1 (ko) | 2003-03-31 | 2009-03-31 | 에프. 호프만-라 로슈 아게 | 일본 뇌염 바이러스 혈청군의 구성원을 포함하는, 특정플라비바이러스 검출용 조성물 및 방법 |
| JP2007534308A (ja) * | 2003-11-19 | 2007-11-29 | アレロジック・バイオサイエンシズ・コーポレーション | 複数のフルオロフォアで標識したオリゴヌクレオチド |
| US20050250134A1 (en) * | 2004-03-02 | 2005-11-10 | Dawei Sheng | Fluorescent energy transfer labeled nucleic acid substrates and methods of use thereof |
| US7939251B2 (en) | 2004-05-06 | 2011-05-10 | Roche Molecular Systems, Inc. | SENP1 as a marker for cancer |
| WO2006086499A2 (en) * | 2005-02-08 | 2006-08-17 | Applera Corporation | Nucleozymes and methods of use |
| EP1863908B1 (de) | 2005-04-01 | 2010-11-17 | Qiagen GmbH | Reverse transkription und amplifikation von rna bei simultaner degradierung von dna |
| US20070015180A1 (en) * | 2005-05-02 | 2007-01-18 | Stratagene California | Oligonucleotide probe/primer compositions and methods for polynucleotide detection |
| EP1762627A1 (de) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion |
| BRPI0616466B1 (pt) | 2005-10-07 | 2021-01-26 | Johnson & Johnson Research Pty Limited | composição de enzimas de ácido nucleico multicomponentes (mnazima), métodos para detectar a presença de pelo menos um facilitador de combinação, um alvo e uma variante de sequência de ácidos nucléicos, métodos para fabricar uma pluraridade de mnazima, testar sequências de núcleo catalítico parcial e identificar suas posições, bem como uso de pelo menos um oligonucleotídeo |
| WO2007056312A2 (en) * | 2005-11-07 | 2007-05-18 | Clontech Laboratories, Inc. | Universal reporter tag for nucleozyme assays |
| AU2006323930B2 (en) * | 2005-12-07 | 2012-06-21 | Janssen Sciences Ireland Uc | Methods, plasmid vectors and primers for assessing HIV viral fitness |
| WO2008001376A2 (en) * | 2006-06-28 | 2008-01-03 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Detection of analytes in a medium |
| DE102006035600B3 (de) * | 2006-07-27 | 2007-11-08 | Epigenomics Ag | Verfahren zum Nachweis eines Methylierungsmusters |
| EP1882746A3 (en) * | 2006-07-27 | 2008-03-19 | Epigenomics AG | Method for detecting a methylation pattern |
| KR20090098960A (ko) | 2006-10-06 | 2009-09-18 | 존슨 앤드 존슨 리서치 피티와이 리미티드 | 분자 스위치 및 이의 이용 방법 |
| US9938641B2 (en) * | 2006-12-18 | 2018-04-10 | Fluidigm Corporation | Selection of aptamers based on geometry |
| WO2008122084A1 (en) | 2007-04-05 | 2008-10-16 | Johnson & Johnson Research Pty Limited | Nucleic acid enzymes and complexes and methods for their use |
| EP2071035A1 (en) * | 2007-12-13 | 2009-06-17 | Epigenomics AG | Facilitator and method for amplification |
| WO2010147848A2 (en) | 2009-06-15 | 2010-12-23 | Rd Biosciences, Inc. | Kits and methods for selective amplification and detection of nucleic acid targets |
| CN101907573B (zh) * | 2010-08-17 | 2012-08-22 | 复旦大学 | 一种高灵敏度的集成cmos光学生物芯片 |
| WO2012034130A2 (en) | 2010-09-10 | 2012-03-15 | Qiagen | Methods and compositions for nucleic acid detection |
| JP6088487B2 (ja) | 2011-04-15 | 2017-03-01 | ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ | 走査リアルタイムマイクロ流体熱サイクラーと同期熱サイクリング及び走査光学検出の方法 |
| CN104040238B (zh) | 2011-11-04 | 2017-06-27 | 汉迪拉布公司 | 多核苷酸样品制备装置 |
| AU2013202354A1 (en) * | 2012-06-18 | 2014-01-16 | Speedx Pty Ltd | Target detection and signal amplification |
| CN103013953B (zh) * | 2012-10-19 | 2014-09-17 | 中国科学院成都生物研究所 | 一种剪切dna的dna酶 |
| CN105518156A (zh) * | 2013-09-05 | 2016-04-20 | 日本电气方案创新株式会社 | 用于制备样品的方法和用于分析靶标的方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0707076A1 (en) * | 1988-09-30 | 1996-04-17 | Amoco Corporation | RNA template end-linked probe constructs and methods for use |
| WO1996027026A1 (en) * | 1995-02-27 | 1996-09-06 | Intelligene Ltd. | Detection of biomolecules |
| WO1996040994A1 (en) * | 1995-06-07 | 1996-12-19 | Merck & Co., Inc. | Dna polymerase extension assay |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4000159A (en) * | 1974-06-28 | 1976-12-28 | Phillips Petroleum Company | Preparation of n,n-disubstituted thioamides |
| US5176995A (en) * | 1985-03-28 | 1993-01-05 | Hoffmann-La Roche Inc. | Detection of viruses by amplification and hybridization |
| US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US5112734A (en) * | 1989-05-26 | 1992-05-12 | Gene-Trak Systems | Target-dependent synthesis of an artificial gene for the synthesis of a replicatable rna |
| NZ314629A (en) * | 1991-01-17 | 2000-08-25 | Gen Hospital Corp | Use trans-splicing ribozymes to prepare medicaments for gene therapies |
| GB9115909D0 (en) * | 1991-07-23 | 1991-09-04 | Nickerson Int Seed | Recombinant dna |
| ATE217347T1 (de) * | 1992-12-04 | 2002-05-15 | Univ Yale | Diagnose mittels signalverstärkung durch ein ribozym |
| US5436143A (en) * | 1992-12-23 | 1995-07-25 | Hyman; Edward D. | Method for enzymatic synthesis of oligonucleotides |
| US5516664A (en) * | 1992-12-23 | 1996-05-14 | Hyman; Edward D. | Enzymatic synthesis of repeat regions of oligonucleotides |
| KR100218726B1 (ko) * | 1992-12-30 | 1999-09-01 | 김영환 | 고집적 반도체 소자의 접속장치 및 그 제조방법 |
| IL105894A (en) * | 1993-06-03 | 1997-02-18 | Asher Nathan | Method for the detection of nucleic acid sequence by using a detection ensemble and an amplification ensemble |
| US5582988A (en) * | 1994-09-15 | 1996-12-10 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same |
| US5807718A (en) * | 1994-12-02 | 1998-09-15 | The Scripps Research Institute | Enzymatic DNA molecules |
| IL111857A (en) * | 1994-12-02 | 1999-09-22 | Intelligene Ltd | Detection of nucleic acid sequences |
| AUPN245295A0 (en) * | 1995-04-13 | 1995-05-11 | Johnson & Johnson Research Pty. Limited | Assay for genetic abnormalities |
| CA2287520C (en) * | 1997-04-29 | 2010-12-14 | The Scripps Research Institute | Enzymatic dna molecules |
| DE19805593A1 (de) * | 1998-02-12 | 1999-08-19 | Voith Sulzer Papiertech Patent | Eindicker |
| CA2321948A1 (en) | 1998-03-05 | 1999-09-10 | Johnson & Johnson Research Pty. Limited | Zymogenic nucleic acid detection methods, and related molecules and kits |
-
1999
- 1999-02-22 CA CA002321948A patent/CA2321948A1/en not_active Abandoned
- 1999-02-22 IL IL13812699A patent/IL138126A0/xx not_active IP Right Cessation
- 1999-02-22 EP EP99914694A patent/EP1062367B1/en not_active Expired - Lifetime
- 1999-02-22 IL IL16418299A patent/IL164182A0/xx unknown
- 1999-02-22 BR BR9908567-4A patent/BR9908567A/pt not_active Application Discontinuation
- 1999-02-22 CN CNB998111147A patent/CN1167811C/zh not_active Expired - Fee Related
- 1999-02-22 AU AU33409/99A patent/AU768627B2/en not_active Ceased
- 1999-02-22 US US09/253,955 patent/US6140055A/en not_active Expired - Lifetime
- 1999-02-22 JP JP2000534677A patent/JP4318856B2/ja not_active Expired - Fee Related
- 1999-02-22 DE DE69939896T patent/DE69939896D1/de not_active Expired - Fee Related
- 1999-02-22 AT AT99914694T patent/ATE414169T1/de not_active IP Right Cessation
- 1999-02-22 KR KR1020007009783A patent/KR100748863B1/ko not_active Expired - Fee Related
- 1999-02-22 WO PCT/IB1999/000754 patent/WO1999045146A1/en not_active Ceased
- 1999-03-04 ZA ZA9901772A patent/ZA991772B/xx unknown
-
2000
- 2000-08-11 US US09/637,405 patent/US6201113B1/en not_active Expired - Lifetime
- 2000-12-21 US US09/746,985 patent/US6365724B2/en not_active Expired - Lifetime
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0707076A1 (en) * | 1988-09-30 | 1996-04-17 | Amoco Corporation | RNA template end-linked probe constructs and methods for use |
| WO1996027026A1 (en) * | 1995-02-27 | 1996-09-06 | Intelligene Ltd. | Detection of biomolecules |
| WO1996040994A1 (en) * | 1995-06-07 | 1996-12-19 | Merck & Co., Inc. | Dna polymerase extension assay |
Also Published As
| Publication number | Publication date |
|---|---|
| DE69939896D1 (de) | 2008-12-24 |
| KR100748863B1 (ko) | 2007-08-13 |
| CA2321948A1 (en) | 1999-09-10 |
| IL164182A0 (en) | 2005-12-18 |
| ATE414169T1 (de) | 2008-11-15 |
| CN1319142A (zh) | 2001-10-24 |
| JP4318856B2 (ja) | 2009-08-26 |
| EP1062367A4 (en) | 2003-07-30 |
| US20010001063A1 (en) | 2001-05-10 |
| JP2002505117A (ja) | 2002-02-19 |
| AU3340999A (en) | 1999-09-20 |
| CN1167811C (zh) | 2004-09-22 |
| EP1062367A1 (en) | 2000-12-27 |
| ZA991772B (en) | 2000-10-12 |
| BR9908567A (pt) | 2000-12-12 |
| US6140055A (en) | 2000-10-31 |
| EP1062367B1 (en) | 2008-11-12 |
| US6365724B2 (en) | 2002-04-02 |
| IL138126A0 (en) | 2001-10-31 |
| KR20010041591A (ko) | 2001-05-25 |
| WO1999045146A1 (en) | 1999-09-10 |
| US6201113B1 (en) | 2001-03-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU768627B2 (en) | Zymogenic nucleic acid detection methods, and related molecules and kits | |
| US5571673A (en) | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes | |
| JP7221199B2 (ja) | マルチプレックスｐｃｒの実施方法 | |
| KR100702338B1 (ko) | 표적핵산의검출에사용하기위한표지된프라이머및표적핵산의검출 | |
| KR100238835B1 (ko) | 클라미디아 트라코마티스 검출용 조성물 및 검출 방법 | |
| SG178350A1 (en) | Td probe and its uses | |
| JP6638122B2 (ja) | 標的核酸の検出方法及びキット | |
| CN102171366A (zh) | 多重扩增与检测 | |
| US20030165898A1 (en) | Method for concurrent amplification and real time detection of polymorphic nucleic acid sequences | |
| EP1327001B1 (en) | Method of amplifying nucleic acids providing means for strand separation and universal sequence tags | |
| JPH1118785A (ja) | ナイセリア・コノルホエア−特異的オリゴヌクレオチド | |
| AU2009241289A1 (en) | Isothermal detection methods and uses thereof | |
| AU2004201097A1 (en) | Zymogenic nucleic acid detection methods, and related molecules and kits | |
| Hou et al. | Molecular beacons for isothermal fluorescence enhancement by the cleavage of RNase HII from Chlamydia pneumoniae | |
| Kramer et al. | Inventing molecular beacons | |
| Beacons | The PCR Revolution: Basic Technologies and Applications | |
| Hou et al. | A Method for HLA Genotyping Using the Specific Cleavage of DNA-rN1-DNA/DNA with RNase HII from Chlamydia pneumoniae | |
| Antson | Genotyping RNA and DNA using padlock probes | |
| AU2494201A (en) | Method for concurrent amplification and real time detection of polymorphic nucleic acid sequences |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MK6 | Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase | ||
| TH | Corrigenda |
Free format text: IN VOL 14, NO 12, PAGE(S) 1934-1937 UNDER THE HEADING APPLICATIONS LAPSED, REFUSED OR WITHDRAWN PLEASE DELETE ALL REFERENCE TO APPLICATION NO. 33409/99 |
|
| FGA | Letters patent sealed or granted (standard patent) |