CN111551729B - 藻红蛋白免疫荧光探针制备方法 - Google Patents
藻红蛋白免疫荧光探针制备方法 Download PDFInfo
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- CN111551729B CN111551729B CN202010342208.7A CN202010342208A CN111551729B CN 111551729 B CN111551729 B CN 111551729B CN 202010342208 A CN202010342208 A CN 202010342208A CN 111551729 B CN111551729 B CN 111551729B
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- phycoerythrin
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- immunofluorescence
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- sulfhydryl
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- G—PHYSICS
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010342208.7A CN111551729B (zh) | 2020-04-27 | 2020-04-27 | 藻红蛋白免疫荧光探针制备方法 |
| PCT/CN2020/092719 WO2021217768A1 (fr) | 2020-04-27 | 2020-05-27 | Procédé de préparation d'une sonde d'immunofluorescence de phycoérythrine |
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|---|---|---|---|
| CN202010342208.7A CN111551729B (zh) | 2020-04-27 | 2020-04-27 | 藻红蛋白免疫荧光探针制备方法 |
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| Publication Number | Publication Date |
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| CN111551729A CN111551729A (zh) | 2020-08-18 |
| CN111551729B true CN111551729B (zh) | 2021-02-09 |
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Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN111551729B (fr) |
| WO (1) | WO2021217768A1 (fr) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112098640B (zh) * | 2020-09-16 | 2021-12-14 | 浙江正熙生物技术股份有限公司 | 荧光蛋白和/或偶联蛋白单克隆抗体标记方法及其试剂盒 |
| CN112745378A (zh) * | 2020-10-23 | 2021-05-04 | 杭州联科生物技术股份有限公司 | 一种藻红蛋白(PE)与Annexin V蛋白偶联方法 |
| CN114377154B (zh) * | 2021-12-08 | 2023-07-07 | 中国科学院上海高等研究院 | 一种基于同步辐射光源的x射线荧光及荧光的双模态成像探针、制备方法及应用 |
| CN117269473B (zh) * | 2023-08-16 | 2024-09-20 | 星童医疗技术(苏州)有限公司 | 用于放大信号的生物探针包被方法 |
Citations (6)
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| CN101750482A (zh) * | 2008-12-17 | 2010-06-23 | 上海海洋大学 | 一种制备条斑紫菜r-藻红蛋白荧光探针的方法 |
| CN101871937A (zh) * | 2010-06-18 | 2010-10-27 | 中国检验检疫科学研究院 | 多种小分子化合物的同步间接竞争免疫检测法及试剂盒 |
| CN103087197A (zh) * | 2013-01-21 | 2013-05-08 | 集美大学 | 一种藻红蛋白标记抗sti抗体、制备方法及其用途 |
| WO2013113501A1 (fr) * | 2012-01-31 | 2013-08-08 | Curevac Gmbh | Composition pharmaceutique comprenant un complexe cargo-support polymère et au moins un antigène protéinique ou peptidique |
| CN105738169A (zh) * | 2014-12-09 | 2016-07-06 | 中国科学院大连化学物理研究所 | 一种蛋白质n-端富集方法 |
| CN110669141A (zh) * | 2019-09-29 | 2020-01-10 | 杭州联科生物技术股份有限公司 | 一种别藻蓝蛋白(APC)与Annexin V蛋白偶联方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2735238B1 (fr) * | 1995-06-09 | 1997-09-05 | Cis Bio Int | Utilisation d'un complexe phycobiliproteine-peptide de liaison en tant que traceur fluorescent |
| MXPA06006659A (es) * | 2003-12-19 | 2006-08-31 | Curis Inc | Composicion y metodos para modular la actividad del sistema nervioso central. |
| CN1786024B (zh) * | 2004-12-10 | 2010-12-15 | 骆建华 | 藻红蛋白、晶体生产工艺及制品 |
| EP2328613A1 (fr) * | 2008-08-28 | 2011-06-08 | GlaxoSmithKline Biologicals S.A. | Vaccin |
| CN101957377B (zh) * | 2010-09-17 | 2013-11-13 | 山东省农业科学院畜牧兽医研究所 | 一种检测禽流感病毒的荧光抗体的制备方法及固相免疫荧光检测试剂盒 |
| CN109142561A (zh) * | 2018-07-17 | 2019-01-04 | 上海师范大学 | 同时定量蛋白质丰度与半胱氨酸氧化水平的方法及其应用 |
-
2020
- 2020-04-27 CN CN202010342208.7A patent/CN111551729B/zh active Active
- 2020-05-27 WO PCT/CN2020/092719 patent/WO2021217768A1/fr active Application Filing
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101750482A (zh) * | 2008-12-17 | 2010-06-23 | 上海海洋大学 | 一种制备条斑紫菜r-藻红蛋白荧光探针的方法 |
| CN101871937A (zh) * | 2010-06-18 | 2010-10-27 | 中国检验检疫科学研究院 | 多种小分子化合物的同步间接竞争免疫检测法及试剂盒 |
| WO2013113501A1 (fr) * | 2012-01-31 | 2013-08-08 | Curevac Gmbh | Composition pharmaceutique comprenant un complexe cargo-support polymère et au moins un antigène protéinique ou peptidique |
| CN103087197A (zh) * | 2013-01-21 | 2013-05-08 | 集美大学 | 一种藻红蛋白标记抗sti抗体、制备方法及其用途 |
| CN105738169A (zh) * | 2014-12-09 | 2016-07-06 | 中国科学院大连化学物理研究所 | 一种蛋白质n-端富集方法 |
| CN110669141A (zh) * | 2019-09-29 | 2020-01-10 | 杭州联科生物技术股份有限公司 | 一种别藻蓝蛋白(APC)与Annexin V蛋白偶联方法 |
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| Wolfgang Bergmeier 等.Flow Cytometric Detection of Activated Mouse Integrin αIIbβ3 With a Novel Monoclonal Antibody.《Cytometry》.2002,第48卷80-86. * |
| 藻红蛋白标记抗鸡IgG 荧光抗体的高效制备;颜世敢 等;《中国预防兽医学报》;20090228;第31卷(第2期);127-131 * |
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| Publication number | Publication date |
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| CN111551729A (zh) | 2020-08-18 |
| WO2021217768A1 (fr) | 2021-11-04 |
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