CN111551729B - Preparation method of phycoerythrin immunofluorescence probe - Google Patents
Preparation method of phycoerythrin immunofluorescence probe Download PDFInfo
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- CN111551729B CN111551729B CN202010342208.7A CN202010342208A CN111551729B CN 111551729 B CN111551729 B CN 111551729B CN 202010342208 A CN202010342208 A CN 202010342208A CN 111551729 B CN111551729 B CN 111551729B
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- phycoerythrin
- target protein
- immunofluorescence
- sulfydryl
- sulfhydryl
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G01N2015/144—Imaging characterised by its optical setup
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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CN202010342208.7A CN111551729B (en) | 2020-04-27 | 2020-04-27 | Preparation method of phycoerythrin immunofluorescence probe |
PCT/CN2020/092719 WO2021217768A1 (en) | 2020-04-27 | 2020-05-27 | Method for preparing phycoerythrin immunofluorescence probe |
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CN202010342208.7A CN111551729B (en) | 2020-04-27 | 2020-04-27 | Preparation method of phycoerythrin immunofluorescence probe |
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CN111551729B true CN111551729B (en) | 2021-02-09 |
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WO (1) | WO2021217768A1 (en) |
Families Citing this family (4)
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CN112098640B (en) * | 2020-09-16 | 2021-12-14 | 浙江正熙生物技术股份有限公司 | Fluorescent protein and/or coupled protein monoclonal antibody marking method and kit thereof |
CN112745378A (en) * | 2020-10-23 | 2021-05-04 | 杭州联科生物技术股份有限公司 | Phycoerythrin (PE) and Annexin V protein coupling method |
CN114377154B (en) * | 2021-12-08 | 2023-07-07 | 中国科学院上海高等研究院 | X-ray fluorescence and fluorescence bimodal imaging probe based on synchrotron radiation light source, preparation method and application |
CN117269473B (en) * | 2023-08-16 | 2024-09-20 | 星童医疗技术(苏州)有限公司 | Biological probe coating method for amplifying signal |
Citations (6)
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CN101750482A (en) * | 2008-12-17 | 2010-06-23 | 上海海洋大学 | Method for preparing porphyra yezoensis R-phycoerythrin fluorescence probe |
CN101871937A (en) * | 2010-06-18 | 2010-10-27 | 中国检验检疫科学研究院 | Synchronous and indirect competitive immunological detection method and kit of plural small molecular compounds |
CN103087197A (en) * | 2013-01-21 | 2013-05-08 | 集美大学 | Phycoerythrin-labeled anti-STI (soybean trypsin inhibitor) antibody, preparation method and use thereof |
WO2013113501A1 (en) * | 2012-01-31 | 2013-08-08 | Curevac Gmbh | Pharmaceutical composition comprising a polymeric carrier cargo complex and at least one protein or pepide antigen |
CN105738169A (en) * | 2014-12-09 | 2016-07-06 | 中国科学院大连化学物理研究所 | Protein N-end enrichment method |
CN110669141A (en) * | 2019-09-29 | 2020-01-10 | 杭州联科生物技术股份有限公司 | Allophycocyanin (APC) and Annexin V protein coupling method |
Family Cites Families (6)
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FR2735238B1 (en) * | 1995-06-09 | 1997-09-05 | Cis Bio Int | USE OF A BINDING PHYCOBILIPROTEIN-PEPTIDE COMPLEX AS A FLUORESCENT TRACER |
MXPA06006659A (en) * | 2003-12-19 | 2006-08-31 | Curis Inc | Composition and methods for modulating cns activity. |
CN1786024B (en) * | 2004-12-10 | 2010-12-15 | 骆建华 | Production technology of phycoerythrin and crystal and product thereof |
EP2328613A1 (en) * | 2008-08-28 | 2011-06-08 | GlaxoSmithKline Biologicals S.A. | Vaccine |
CN101957377B (en) * | 2010-09-17 | 2013-11-13 | 山东省农业科学院畜牧兽医研究所 | Method for preparing fluorescent antibody for detecting avian influenza virus and solid phase immunofluorescence detection kit |
CN109142561A (en) * | 2018-07-17 | 2019-01-04 | 上海师范大学 | The method and its application of simultaneous quantitative protein abundance and cysteine oxidation level |
-
2020
- 2020-04-27 CN CN202010342208.7A patent/CN111551729B/en active Active
- 2020-05-27 WO PCT/CN2020/092719 patent/WO2021217768A1/en active Application Filing
Patent Citations (6)
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CN101750482A (en) * | 2008-12-17 | 2010-06-23 | 上海海洋大学 | Method for preparing porphyra yezoensis R-phycoerythrin fluorescence probe |
CN101871937A (en) * | 2010-06-18 | 2010-10-27 | 中国检验检疫科学研究院 | Synchronous and indirect competitive immunological detection method and kit of plural small molecular compounds |
WO2013113501A1 (en) * | 2012-01-31 | 2013-08-08 | Curevac Gmbh | Pharmaceutical composition comprising a polymeric carrier cargo complex and at least one protein or pepide antigen |
CN103087197A (en) * | 2013-01-21 | 2013-05-08 | 集美大学 | Phycoerythrin-labeled anti-STI (soybean trypsin inhibitor) antibody, preparation method and use thereof |
CN105738169A (en) * | 2014-12-09 | 2016-07-06 | 中国科学院大连化学物理研究所 | Protein N-end enrichment method |
CN110669141A (en) * | 2019-09-29 | 2020-01-10 | 杭州联科生物技术股份有限公司 | Allophycocyanin (APC) and Annexin V protein coupling method |
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WO2021217768A1 (en) | 2021-11-04 |
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Address after: Building 3, Building 1, No. 926, Changhong East Street, Fuxi Street, Deqing County, Huzhou City, Zhejiang Province (Moganshan National High-tech Zone) (self declaration) Patentee after: Zhejiang Zhengxi Biotechnology Co.,Ltd. Address before: Building 1, No. 926, Changhong East Street, Fuxi street, Deqing County, Huzhou City, Zhejiang Province, 313000 (Moganshan national high tech Zone) Patentee before: Zhejiang Zhengxi Biotechnology Co.,Ltd. |
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PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Preparation method of phycoerythrin immunofluorescence probe Granted publication date: 20210209 Pledgee: Deqing sub branch of Bank of China Ltd. Pledgor: Zhejiang Zhengxi Biotechnology Co.,Ltd. Registration number: Y2024980010535 |