CN108207504B - Method for breeding marsdenia tenacissima seedlings by using nutrition bags - Google Patents
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Abstract
The invention discloses a method for breeding marsdenia tenacissima seedlings by nutrient bags, which comprises the steps of selecting land, building a bed, preparing nutrient soil, preparing nutrient bagged soil, placing, selecting seeds, dibbling/transplanting seedlings, managing a seedbed, transplanting the marsdenia tenacissima seedlings and the like, and breeding the marsdenia tenacissima seedlings by the measures of building a plurality of seedbeds, preparing nutrient soil, selecting seeds, dibbling/transplanting seedlings, managing the seedbed, transplanting the marsdenia tenacissima seedlings and the like. Compared with the traditional conventional seedling transplanting method, the survival rate of the seedlings can be remarkably improved by more than 90%, the seedlings cultured by the method are transplanted without weather limitation, the seedling revival period of the marsdenia tenacissima seedlings can be eliminated, and the biomass of the marsdenia tenacissima in the transplanting year is improved by more than 60%.
Description
Technical Field
The invention relates to the technical field of Chinese medicinal material breeding, in particular to a method for breeding marsdenia tenacissima seedlings by using nutrient bags.
Background
Caulis Marsdeniae Tenacissimae is a perennial vine of the genus California of the family Asclepiadaceae, and its stem, root and leaf have the effects of diminishing inflammation, clearing away heat and toxic materials, etc. Recent researches show that chemical components such as marsdenia tenacissima C21 steroids have an anti-tumor effect, so that the demand of the marsdenia tenacissima is gradually increased, and the traditional medicinal material supply mainly comes from wild natural breeding and cannot meet the rapidly-increasing medicinal material demand.
The marsdenia tenacissima can be propagated in two modes of sexual seed propagation and asexual cuttage propagation. In the current cultivation, seedlings are mainly propagated through seeds, and then seedling transplantation and branch cuttage propagation are carried out. However, cutting propagation cuttings are easy to rot and not easy to root, the requirements on the cutting environment are strict, seedling raising and transplanting of seeds have certain requirements on soil humidity and climatic conditions, the survival rate after transplanting is relatively low, and the seedling revival period is relatively long.
The seedlings bred by the marsdenia tenacissima nutrition bag are mainly used as wild tending and transplanting seedlings, and the wild marsdenia tenacissima can grow in the barren fields with more rocks. Therefore, the wild tending planting land selection is usually serious in desertification, weak in water storage capacity and unfavorable for irrigation karst landform, and the effect of environmental protection can be achieved through ecological planting while the growth of the marsdenia tenacissima is met. Therefore, a seedling raising mode is explored, the emergence rate and the seedling growth condition of seeds can be guaranteed, the survival rate after transplanting can be guaranteed, and the method has important significance for guaranteeing the planting amplification and the raw material supply of marsdenia tenacissima.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide a method for breeding marsdenia tenacissima seedlings by using a nutrition bag.
According to the purpose of the invention, the method for breeding the Marsdenia tenacissima seedlings by the nutrient bags comprises the following steps:
step 1, selectively building a bed: selecting a land block with flat terrain, good drainage and ventilation, sufficient illumination, convenient irrigation and convenient traffic, and establishing a plurality of seedbeds;
step 2, preparing nutrient soil: the nutrient soil comprises vermiculite, decomposed farmyard manure and loam, the nutrient soil is mixed according to the proportion that the vermiculite, the decomposed farmyard manure and the loam are 1: 2: 7, then a high phosphorus and potassium compound fertilizer I is added into the nutrient soil according to the proportion of 2% to prepare a soil-fertilizer mixture, and finally 10% of fosthiazate granules are mixed into the soil-fertilizer mixture, wherein the application amount is 15-20 g/m3(ii) a In the high phosphorus potassium compound fertilizer I, the content of N is 5 percent and P is calculated by mass fraction2O5Content 15%, K2The O content is 15 percent;
step 3, bagging and placing the nutrition bags: watering and wetting the nutrient soil before bagging to ensure that the water content of the nutrient soil reaches 50-60 percent, and then filling the nutrient soil into the nutrient bag; the nutrient bags are divided into dibbling bags and transplanting bags according to the soil loading amount of the nutrient soil, the total capacity of the dibbling bags is 4/5 of the nutrient soil, and the total capacity of the transplanting bags is 3/5 of the nutrient soil; placing the nutrition bag filled with the nutrition soil on a seedbed, and ridging and fixing the periphery of the seedbed;
step 4, seed selection, dibbling/seedling transplanting: the method comprises the following steps of (1) adopting two modes of seed dibbling and seedling transplanting for the marsdenia tenacissima seedling breeding by a nutrition bag;
and (3) seed dibbling: selecting a dibbling bag filled with total nutrient soil of 4/5, opening a pit with the diameter of 2-3 cm and the depth of 1cm, dibbling 3 treated marsdenia tenacissima seeds, backfilling with the nutrient soil, and watering thoroughly;
transplanting seedlings: selecting marsdenia tenacissima seedlings which grow for 20-25 days and have at least three pairs of true leaves and a plant height of 5-8 cm, transplanting the marsdenia tenacissima seedlings into a transplanting bag filled with 3/5 nutrient soil of the total volume of the nutrient bag, filling the nutrient soil again to 4/5 of the total volume of the nutrient bag, and watering thoroughly;
step 5, seedbed management: thinning after the seeds in the dibbling bags emerge for 30 days, wherein each nutrition bag only keeps a seedling with the best growth, and an amino acid water-soluble fertilizer is selected and diluted by 500 times to be sprayed once; every 25 days, root-applying 1g/L of the compound water-soluble fertilizer, and simultaneously using EDTA chelated micro-fertilizer containing 0.15% of Zn, Cu and Fe respectively, diluting by 1000 times and spraying once; applying the compound water-soluble fertilizer 1g/L once again at intervals of 30 days; the content of N in the composite water-soluble fertilizer is 3 percent by mass, and P is2O5Content 5%, K2The O content is 6 percent; the amino acid water-soluble fertilizer contains various organic micromolecular active ingredients, such as preparations for promoting plant growth, such as 'root-growth-promoting' and the like. The spraying amount of the amino acid water-soluble fertilizer and the chelated trace fertilizer is that the plant leaves just drop but do not fall, and the root application of the compound water-soluble fertilizer is carried out simultaneously by combining watering, wherein 300-500 ml of each plant is sprayed.
After the seedlings in the transplanting bag are transplanted for 10 days, amino acid water-soluble fertilizer is used for diluting by 500 times and spraying once; every 25 days, root-applying 1g/L of the compound water-soluble fertilizer, simultaneously selecting EDTA chelated micro-fertilizer containing 0.15% of Zn, Cu and Fe, diluting by 1000 times and spraying once; applying the compound water-soluble fertilizer 1g/L once again at intervals of 30 days; the content of N in the water-soluble fertilizer is 3 percent by mass, and P is25% O5 content, K2The O content is 6 percent; the amino acid water-soluble fertilizer contains a plurality of amino acid water-soluble fertilizersThe plant growth promoting preparation contains organic small molecule active components, such as 'root-rich' and the like. The spraying amount of the amino acid water-soluble fertilizer and the chelated trace fertilizer is that the plant leaves just drop but do not fall, and the root application of the compound water-soluble fertilizer is carried out simultaneously by combining watering, wherein 300-500 ml of each plant is sprayed.
Within 30 days after the seeds in the dibbling bag emerge, transplanting the seedlings in the transplanting bag for 10 days, and watering once every 3-4 days; then watering once every 5-6 days until seedlings are transplanted, wherein each watering requires the soil water content to reach more than 70%; the whole seedling stage is not timed and is matched with artificial weeding, so that no weed is generated in the nutrition bag and on the seedbed;
after seedlings are completely emerged, the wettable powder with carbendazim of 25 percent is diluted by 500 to 1000 times and sprayed once, and after 10 days, the wettable powder with chlorothalonil of 75 percent is diluted by 500 to 600 times and sprayed once; irrigating roots with 500-800 times of 58% metalaxyl wettable powder after 20 days; when aphids appear, plant ash and water are used for soaking according to the ratio of 1: 5, and the plant ash and water are sprayed to affected plants with the aphids;
and (2) applying a compound fertilizer II once (30 kg/mu) to the seedlings in the dibbling bag and the transplanting bag 2-3 days before transplanting, wherein the N content in the compound fertilizer II is 15 percent and the P content in the compound fertilizer II is 30 percent by mass2O5Content 15%, K2The O content is 15 percent;
step 6, transplanting the marsdenia tenacissima seedlings: and after the marsdenia tenacissima seedlings grow to the height of 25-35 cm, transplanting the marsdenia tenacissima seedlings with a nutrition bag after at least five pairs of true leaves.
Further, in the step 1, the elevation of the land where the seedbed is built is 600-900 m, the area of the land is not less than 20m multiplied by 30m, the length of the seedbed is 2-10 m, the width of the seedbed is 60cm, and the distance between the seedbeds is 30 cm.
Further, in the step 2, the soil for preparing the nutrient soil needs to be subjected to impurity removal, sun drying, grinding and sieving by a 2-3 mm sieve.
Further, in the step 3, the nutrition bag is selected from a barrel-shaped nutrition bag with the specification of 8cm in diameter and 10cm in height, and the nutrition bag is made of degradable non-woven fabrics.
Further, in step 4, when the seeds are dibbled, pre-sowing treatment is performed: 3 days before sowing, selecting plump marsdenia tenacissima seeds with the length of 1-1.2 cm and the germination rate of more than 80 percent, and placing0.5% KMnO4Sterilizing for 30min, washing twice with clear water, soaking in 35 deg.c warm water for 4 hr, taking out and drying at room temperature for 24 hr, and seeding for 3-4-20 days each year.
Further, in the step 4, before the seeds are dibbled, a sunshade net with the height not less than 1.5m is erected above the seedbed, and the sunshade net is uncovered every 25-30 days after the seedlings in the dibble seeding bag are fully grown.
Further, the light transmittance of the sunshade net is 70% -80%.
Furthermore, the compound fertilizer II is added by preparing matrix soil, and nutrient supply is ensured by applying water-soluble fertilizer and high-efficiency EDTA chelated micro-fertilizer.
The invention has the beneficial effects that:
1. compared with the traditional method for seedling raising and transplanting of the Marsdenia tenacissima by using the seed direct seeding seedbed, the method for seedling raising and transplanting of Marsdenia tenacissima can realize transplanting in rainy and sunny days, the survival rate can reach more than 90%, the seedling revival stage is eliminated, and the biomass of Marsdenia tenacissima is increased by more than 60% in the current year after transplanting.
2. The method for breeding the Marsdenia tenacissima seedlings by the nutrition bags can be used for breeding the Marsdenia tenacissima seedlings on a large scale, the breeding survival rate is high, the breeding speed is high, the yield and the quality are improved, and the market medication pressure is relieved.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to specific embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for breeding Marsdenia tenacissima seedlings by using nutrient bags comprises the following steps:
step 1, establishing a seedbed: in 2017, in 3-4 months, selecting a land area between 21 degrees 00 'and 23 degrees 59' of north latitude, namely Honghezhou Mongyuan city, Yunnan province, and establishing a plurality of seedbeds in the land area which has the altitude of 600-900 m, flat terrain, good drainage and ventilation, sufficient illumination, convenience for irrigation and convenience for traffic, wherein the area of the land area is not less than 20m multiplied by 30m, the length of each seedbed is 2-3 m, the width of each seedbed is 60cm, and the distance between the seedbeds is 30 cm;
step 2, preparing nutrient soil: the nutrient soil is mainly formed by mixing vermiculite, decomposed farmyard manure and loam, the nutrient soil is firstly mixed according to the mass ratio of 1: 2: 7 of the vermiculite to the decomposed farmyard manure to the loam, then a high-phosphorus potassium compound fertilizer I is added into the nutrient soil according to the proportion of 2% to prepare a soil-fertilizer mixture, and finally 15-20 g/m of the soil-fertilizer mixture is added3The amount of the fosthiazate granules is 10 percent of the mixture of the soil and the fertilizer; in the high phosphorus potassium compound fertilizer I, the content of N is 5 percent and P is calculated by mass fraction2O5Content 15%, K2The O content is 15 percent;
step 3, bagging the nutrient soil, and placing: a nutrition bag made of degradable non-woven fabrics in a barrel shape with the diameter of 8cm and the height of 10cm is selected, before bagging, the nutrition soil is watered and mixed with water, the water content of the nutrition soil is 50% -60%, and then the nutrition soil is filled into the nutrition bag. The nutrition bags are divided into dibbling bags and transplanting bags according to the soil loading amount of the nutrition soil, the soil loading amount of the dibbling bags is 4/5 of the total capacity of the nutrition bags, the transplanting bags are 3/5 of the total capacity of the nutrition bags, the nutrition bags containing the nutrition soil are placed on a seedbed, and the periphery of the seedbed is earthed and fixed;
step 4, seed selection, dibbling/seedling transplanting: the method comprises the following steps of (1) adopting two modes of seed dibbling and seedling transplanting for the marsdenia tenacissima seedling breeding by a nutrition bag;
and (3) seed dibbling: before seed dibbling, a sunshade net with the height not less than 1.5m is erected above the seedbed, and the light transmittance of the sunshade net is 70-80%; performing pretreatment before sowing: 3 days before sowing, selecting plump marsdenia tenacissima seeds with the length of 1-1.2 cm and the germination rate of more than 80 percent, and putting 0.5 percent of KMnO4Disinfecting the solution for 30min, washing the solution twice with clear water, then transferring the solution into warm water at 35 ℃ to soak the solution for 4h, taking the solution out, drying the solution at room temperature for 24h, and sowing the seeds for 3-4-20 days; selecting a dibble seeding bag filled with total nutrient soil of 4/5, opening a pit with the depth of 1cm by using a stick with the diameter of 2-3 cm, dibbling 3 treated seeds, backfilling the nutrient soil, and then watering thoroughly; after the seedlings in the dibble seeding bag are completely grownRemoving the sunshade net at intervals of 25-30 days;
transplanting seedlings: selecting marsdenia tenacissima seedlings which grow for 20-25 days and have at least three pairs of true leaves and a plant height of 5-8 cm, transplanting the marsdenia tenacissima seedlings into a transplanting bag filled with 3/5 nutrient soil of the total volume of the nutrient bag, filling the nutrient soil again to 4/5 of the total volume of the nutrient bag, and watering thoroughly;
step 5, seedbed management: thinning after the seeds in the dibbling bags emerge for 30 days, wherein each nutrition bag only keeps a seedling with the best growth, and then diluting with more than one root for 500 times and spraying once; every 25 days, root-applying 1g/L of the compound water-soluble fertilizer once, simultaneously spraying 0.15% of EDTA chelated micro fertilizer containing Zn, Cu and Fe once, diluting 1000 times and spraying; applying the compound water-soluble fertilizer 1g/L once again at intervals of 30 days; the spraying amount of the much root and the chelated micro-fertilizer is that the plant leaves just drop but do not fall, and the root application of the compound water-soluble fertilizer is carried out simultaneously in combination with watering, wherein 300-500 ml of the compound water-soluble fertilizer is sprayed on each plant; the roots are mostly amino acid water-soluble fertilizers containing various organic micromolecular active ingredients; the content of N in the composite water-soluble fertilizer is 3 percent by mass, and P is2O5Content 5%, K2The O content is 6 percent;
spraying more roots once after 10 days of seedling transplantation in the transplanting bag, and diluting by 500 times; every 25 days, root-applying 1g/L of the compound water-soluble fertilizer once, simultaneously spraying 0.15% of EDTA chelated micro fertilizer containing Zn, Cu and Fe once, diluting 1000 times and spraying; applying the compound water-soluble fertilizer 1g/L once again at intervals of 30 days; the spraying amount of the much root and the chelated micro-fertilizer is that the plant leaves just drop but do not fall, and the root application of the compound water-soluble fertilizer is carried out simultaneously in combination with watering, wherein 300-500 ml of the compound water-soluble fertilizer is sprayed on each plant; the roots are mostly amino acid water-soluble fertilizers containing various organic micromolecular active ingredients; the content of N in the composite water-soluble fertilizer is 3 percent by mass, and P is2O5Content 5%, K2The O content is 6 percent;
within 30 days after the seeds in the dibbling bag emerge, transplanting the seedlings in the transplanting bag for 10 days, and watering once every 3-4 days; and then watering once every 5-6 days until seedlings are transplanted, wherein each watering requires the soil water content to reach more than 70%. The whole seedling stage is not timed and is matched with artificial weeding, so that no weed is generated in the nutrition bag and on the seedbed;
after seedlings are completely emerged, the wettable powder with carbendazim of 25 percent is diluted by 500 to 1000 times and sprayed once, and after 10 days, the wettable powder with chlorothalonil of 75 percent is diluted by 500 to 600 times and sprayed once; irrigating roots with 500-800 times of 58% metalaxyl wettable powder after 20 days; when aphids appear, plant ash and water are used for soaking according to the ratio of 1: 5, and the plant ash and water are sprayed to affected plants with the aphids;
and (2) applying a compound fertilizer II once (30 kg/mu) to the seedlings in the dibbling bag and the transplanting bag 2-3 days before transplanting, wherein the N content in the compound fertilizer II is 15 percent and the P content in the compound fertilizer II is 30 percent by mass2O5Content 15%, K2The O content is 15 percent;
step 6, transplanting the marsdenia tenacissima seedlings: in 7-month-middle ten days in 2017, when the marsdenia tenacissima seedlings grow to the height of 25-35 cm, transplanting the marsdenia tenacissima seedlings by connecting nutrient bags after at least five pairs of true leaves, and preferably selecting rainy seasons when the plants are transplanted, wherein the soil humidity reaches more than 50%, and the soil humidity and rainfall can be maximally guaranteed.
Example 2
The statistical results of the survival rates of the seedlings in the dibble seeding bag and the transplanting bag are shown in table 1:
in table 1, the seedling storage rate is the number of stored seedlings during seedling transplantation compared with the number of seedlings emerging from the middle of the dibble seeding bag or the number of transplanted seedlings in the transplanting bag.
TABLE 1 survival rate table of seedlings
| Dibble seeding bag | Transplanting bag | |
| Number of seedlings or transplanting | 1440 | 1800 |
| Number of seedlings stored (plant) | 1298 | 1694 |
| Rate of stock of seedlings | 90.1％ | 94.1％ |
Referring to table 1, the marsdenia tenacissima seedling survival rate of the dibbling bag and the transplanting bag is over 90%.
Example 3
Referring to table 2, the total biomass and the seedling height in three months of transplanting of the nutrition bag seedling and the seed direct seeding seedling are investigated and compared, and the test results are shown in table 2:
according to the table 2, compared with direct seeding and seedling raising of seeds, 90 days after transplanting, seedling raising of the nutrition bag eliminates a seedling revival period, the height of seedlings is improved by 71.1%, and biomass is improved by 61%.
In table 2, the direct seeding seedlings of the seeds are raised by a conventional seedling raising method, and during transplanting, healthy seedlings with the height similar to that of the seedlings raised by the embodiment of the invention and the total biomass are manually selected for marking and tracking, and the data of the seedling reviving period, the data of the seedling reviving period after 60 days and the data of the seedling reviving period after 90 days are respectively counted;
the "seedlings grown and transplanted by the invention" referred to in table 2 includes seedlings grown by the dibbling bag and the transplanting bag, and the statistics of the height and total biomass of the seedlings grown and transplanted by the invention in the embodiment are the common statistics of the two seedlings.
Table 2 comparison table of transplanting effect
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (1)
1. A method for breeding Marsdenia tenacissima seedlings by using nutrient bags is characterized by comprising the following steps:
step 1, selectively building a bed: selecting a land block with flat terrain, good drainage and ventilation, sufficient illumination, convenient irrigation and convenient traffic, and establishing a plurality of seedbeds;
the elevation of the plot of the seedbed is 600-900 m, the area of the plot is not less than 20m multiplied by 30m, the length of the seedbed is 2-10 m, the width of the seedbed is 60cm, and the distance between the seedbeds is 30 cm;
step 2, preparing nutrient soil: the nutrient soil comprises vermiculite, decomposed farmyard manure and loam; mixing the nutrient soil according to the proportion of 1: 2: 7 of vermiculite to decomposed farmyard manure to loam, adding a high phosphorus and potassium compound fertilizer I into the nutrient soil according to the proportion of 2% to prepare a soil fertilizer mixture, and finally mixing 10% of fosthiazate granules into the soil fertilizer mixture, wherein the application amount is 15-20 g/m3(ii) a In the high phosphorus potassium compound fertilizer I, the content of N is 5 percent and P is calculated by mass fraction2O5Content 15%, K2The O content is 15 percent;
the soil for preparing the nutrient soil needs to be subjected to impurity removal, sun drying, crushing and screening by a 2-3 mm sieve;
step 3, bagging and placing the nutrition bags: the nutrition bag is a barrel-shaped nutrition bag with the specification of 8cm in diameter and 10cm in height, and is made of degradable non-woven fabrics; watering and wetting the nutrient soil before bagging to ensure that the water content of the nutrient soil reaches 50-60 percent, and then filling the nutrient soil into the nutrient bag; the nutrient bags are divided into dibbling bags and transplanting bags according to the soil loading amount of the nutrient soil, the total capacity of the dibbling bags is 4/5 of the nutrient soil, and the total capacity of the transplanting bags is 3/5 of the nutrient soil; placing the nutrition bag filled with the nutrition soil on a seedbed, and ridging and fixing the periphery of the seedbed;
step 4, seed selection, dibbling/seedling transplanting: the method comprises the following steps of (1) adopting two modes of seed dibbling and seedling transplanting for the marsdenia tenacissima seedling breeding by a nutrition bag;
and (3) seed dibbling: when the seeds are dibbled, the sowing pretreatment is carried out: 3 days before sowing, selecting plump marsdenia tenacissima seeds with the length of 1-1.2 cm and the germination rate of more than 80 percent, and putting 0.5 percent of KMnO4Sterilizing for 30min, washing twice with clear water, then soaking in warm water at 35 ℃ for 4h, taking out, drying at room temperature for 24h, and sowing for 3-4-20 days every year;
selecting a dibbling bag filled with total nutrient soil of 4/5, opening a pit with the diameter of 2-3 cm and the depth of 1cm, dibbling 3 treated marsdenia tenacissima seeds, backfilling with the nutrient soil, and watering thoroughly;
before seed dibbling, a sunshade net with the height not less than 1.5m is erected above the seedbed, and the sunshade net is removed every 25-30 days after seedlings in the dibbling bag are completely grown; the light transmittance of the sunshade net is 70% -80%;
transplanting seedlings: selecting marsdenia tenacissima seedlings which grow for 20-25 days and have at least three pairs of true leaves and a plant height of 5-8 cm, transplanting the marsdenia tenacissima seedlings into a transplanting bag filled with 3/5 nutrient soil of the total volume of the nutrient bag, filling the nutrient soil again to 4/5 of the total volume of the nutrient bag, and watering thoroughly;
step 5, seedbed management: thinning after the seeds in the dibbling bags emerge for 30 days, wherein each nutrition bag only keeps a seedling with the best growth, and an amino acid water-soluble fertilizer is selected and diluted by 500 times for spraying; every 25 days, root-applying 1g/L of the compound water-soluble fertilizer, simultaneously applying EDTA chelated micro fertilizer containing 0.15% of Zn, Cu and Fe once, diluting 1000 times and spraying once; applying the compound water-soluble fertilizer 1g/L once again at intervals of 30 days; the content of N in the composite water-soluble fertilizer is 3 percent by mass, and P is2O5Content 5%, K2The O content is 6 percent;
after the seedlings in the transplanting bag are transplanted for 10 days, amino acid water-soluble fertilizer is used for diluting by 500 times and spraying once; every 25 days, root-applying 1g/L of the compound water-soluble fertilizer, simultaneously selecting EDTA chelated micro-fertilizer containing 0.15% of Zn, Cu and Fe, diluting by 1000 times and spraying once; applying the compound water-soluble fertilizer 1g/L once again at intervals of 30 days; the content of N in the water-soluble fertilizer is 3 percent by mass, and P is2O5Content 5%, K2The content of O is 6 percent；
Within 30 days after the seeds in the dibbling bag emerge, transplanting the seedlings in the transplanting bag for 10 days, and watering once every 3-4 days; then watering once every 5-6 days until seedlings are transplanted, wherein each watering requires the soil water content to reach more than 70%; the whole seedling stage is not timed and is matched with artificial weeding, so that no weed is generated in the nutrition bag and on the seedbed;
after seedlings are completely emerged, the wettable powder with carbendazim of 25 percent is diluted by 500 to 1000 times and sprayed once, and after 10 days, the wettable powder with chlorothalonil of 75 percent is diluted by 500 to 600 times and sprayed once; irrigating roots with 500-800 times of 58% metalaxyl wettable powder after 20 days; when aphids appear, plant ash and water are used for soaking according to the ratio of 1: 5, and the plant ash and water are sprayed to affected plants with the aphids;
and (2) applying a compound fertilizer II once (30 kg/mu) to the seedlings in the dibbling bag and the transplanting bag 2-3 days before transplanting, wherein the N content in the compound fertilizer II is 15 percent and the P content in the compound fertilizer II is 30 percent by mass2O5Content 15%, K2The O content is 15 percent; the compound fertilizer II is added by preparing matrix soil, and nutrient supply is ensured by applying water-soluble fertilizer and efficient EDTA chelated micro-fertilizer;
step 6, transplanting the marsdenia tenacissima seedlings: and after the marsdenia tenacissima seedlings grow to the height of 25-35 cm, transplanting the marsdenia tenacissima seedlings with a nutrition bag after at least five pairs of true leaves.
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