CN104904481A - Cutting seedling-culturing method for France Grasse roses - Google Patents
Cutting seedling-culturing method for France Grasse roses Download PDFInfo
- Publication number
- CN104904481A CN104904481A CN201510335818.3A CN201510335818A CN104904481A CN 104904481 A CN104904481 A CN 104904481A CN 201510335818 A CN201510335818 A CN 201510335818A CN 104904481 A CN104904481 A CN 104904481A
- Authority
- CN
- China
- Prior art keywords
- cutting
- cuttage
- cuttings
- lasi
- french
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a cutting seedling-culturing method for France Grasse roses. The purpose is to solve the problem that rooting of ex-vivo branches of the France Grasse roses is difficult. The method includes the following steps that step1, according to preparation at the early stage, a cutting bed is laid, and cutting media are loaded into non-woven cloth seedling-culturing net bags and comprise, by weight, 30%-35% of vermiculite, 30%-35% of perlite and 35%-40% of grass carbon or rice hull carbon; step2, cutting preparation is performed; step3, according to growth promoting, low cut portions of sheared cutting mother branches are immerged into a growth promoting mixed solution, and soaking is performed; step4, cutting is performed; step5, management after cutting is performed; step6, seedling hardening and transplanting are performed. By the adoption of the method, callus tissue of the roses grows rapidly, and the survival rate is high. The callus tissue starts to be generated at the roots of cuttings after one week from cutting, callus tissue is overgrown at the roots after 15 days, and callus tissue is generated on 95% of branches after 20 days. Meanwhile, rooting and sprouting are rapid at a large quantity; rooting starts on bud eyes on the lowest portion after 15 days from cutting, and the rooting rate reaches above 85% after 30 days.
Description
Technical field
The present invention relates to fast seedling growing cuttage root-taking breeding field, particularly relate to a kind of French Ge Lasi rose cuttage and seedling culture method.
Background technology
France's Ge Lasi (Chiba) rose Sterile culture technology is plant division and propagation by layering, have simple to operate, breed easy feature, but reproduction coefficient is low, damage by disease and insect is many, nursery stock specification is nonstandard is its shortcoming.Cottage propagation has reproduction coefficient high, the good maintenance excellent genes of maternal plant, batch production and standardized workable advantage.But because Ge Lasi (Chiba) rose isolated shoot is taken root difficulty, therefore cottage propagation is the difficult problem on producing always.
Summary of the invention
The object of this invention is to provide a kind of French Ge Lasi rose cuttage and seedling culture method, to solve the difficult problem of taking root of French Ge Lasi rose isolated shoot.
A kind of French Ge Lasi rose cuttage and seedling culture method, comprises the steps:
(1) early-stage preparations:
1.1 conventional methods build green house, and peripheral hardware shading rate is the low-pressure polyethylene sunshade net of 50%-75%;
Behind ground in 1.2 smooth green houses, lay cutting bed; Cutting medium loads non-woven fabrics nursery mesh bag, and abundant moistening rear loading cave dish of spraying water, furnishes successively on cutting bed; Cutting medium is made up of the raw material of following weight percent, vermiculite 30-35%, perlite 30-35%, the peat composed of rotten mosses or rice hull carbon 35-40%;
(2) the preparation of cuttings:
France Ge Lasi rose Post flowering 30-60d, choose then raw new slightly, the thick 0.4-0.6cm of stem, healthy growth without the plant of damage by disease and insect as the female branch of cuttings; The female branch of cuttings is by every 3-4 joints before sunrise, and the branch of long 8-12cm is one section, and tangent plane smooth female branch in clip top and the bottom is as cuttings, and female branch only retains goes up 1 joint, 2-6 compound leaves most;
(3) grow promotion: growth is immersed at the lower cut position of female for the cuttings of clip branch and promotes, in mixed liquor, to soak; Growth promotes that mixed liquor is made up of the raw material of following weight parts: ABT root-inducing powder 45-60 part, plant growth regulator 45-55 part, C
6h
12o
6aqueous solution 5-10 part, Cobastab
12parenteral solution 5-10 part;
(4) by having soaked growth, cuttage: cutting hole is beaten by the cutting medium central authorities in the dish of cave, then promotes that the cuttings cuttage of liquid is in hole;
(5) the management after cuttage:
5.1 spraying and sterilizations: cuttage is sprayed simultaneously, until the blade face of cuttings forms water membrane; Then to blade face sterilization;
5.2 short roots: impose foliage fertilizer once in after Callus formation every 6-8 days;
(6) hardening and transplanting:
After cuttings generally grows side root, carry out hardening according to a conventional method, and 5-7 days before transplanting start progressively to cut off the water; When root system grows described non-woven fabrics nursery mesh bag, can transplant.
Preferably, (1) described step lays cutting bed method in early-stage preparations is successively lay by the width of every 110-120cm clean fine sand that a layer thickness is 2-5cm and thickness is that the cobblestone of 4-8cm is as cutting bed; The working path of reserved 30cm-40cm between cutting bed; Cutting medium through mass concentration be 0.1% potassium permanganate or 200 times of liquid carbendazim spray disinfectants after load non-woven fabrics nursery mesh bag; Above cutting bed, several fog-spray nozzle is laid in middle, and the spacing of adjacent fog-spray nozzle is 1-1.2m.
Preferably, growth promotes that the concentration of each raw material in mixed liquor is as follows, ABT root-inducing powder 100mg/L, plant growth regulator 200mg/L, C
6h
12o
6aqueous solution mass content 5%, Alpha Vee-12 200mg/L.
Preferably, female branch lower cut 1-3mm place and in angle, upper cut is 1-3mm place above the position of the top bud below the position of bottom bud in the preparation of described step (2) cuttings.
Preferably, (3) described step grows in promotion and is immersed in growth promotion mixed liquor by female for cuttings branch lower cut position l-2cm, soaks 60-90min.
Preferably, described step (4) in concrete grammar be cutting medium central authorities make a call to 1-2cm deeply, diameter is the cutting hole of 0.4-0.6cm, then by cuttings cuttage in hole, water spray irrigates cutting medium use have gentle hands light press immediately, and after making to insert, described cutting medium retains 2-3 outward and saves.
Preferably, method for disinfection in the management of described step (5) after cuttage is use 500 times of mancozebs respectively in every 5-7 days, 800 times of carbendazim and 1000 times of tpn mixed liquors replace blade face sterilization, the mass content of carbendazim is 45%-55%, the mass content of tpn is 45%-55%, mixed liquor replaces blade face sterilization, and night carries out.
Preferably, be that temperature 25-30 DEG C, air humidity 80-95%, cutting medium humidity is 50-60% to temperature and humidity requirement in the management of described step (5) after cuttage.
Preferably, the short method for root in the management of described step (5) after cuttage is after Callus formation, and alternately use 0.4% urea and potassium dihydrogen phosphate mixed liquor, ABT root-inducing powder, plant growth regulator, night carries out; The mass content of urea is 45%-55%, and the mass content of potassium dihydrogen phosphate is 45%-55%.
Preferably, carry out illumination control in the management of described step (5) after cuttage, when the temperature in green house is higher than 30 DEG C, cover described sunshade net, all the other time closes.
The present invention compared with prior art has the following advantages:
1, adopt the inventive method, rose callus growth is rapid, and survival rate is high.Cuttage started to produce callus at the base portion of cuttings after one week, and the full callus of 15 space-based ministers, after 20 days, the branch of 95% produces callus., root not only fast meanwhile, Er Qieduo; The eye place of cuttage at foot after 15 days starts to take root, and after 30 days, rooting rate reaches more than 85%.Rose isolated shoot maintains the excellent genes of maternal plant preferably.
2, production efficiency of the present invention is high: the plant of Ge Lasi (Chiba) rose life in 5 years can obtain female 50, the branch of cuttage, the female branch of cuttage of each standard can be cut into 4-5 cuttings, reproduction coefficient reaches 200-400, more than 100 times high compared with division propagation coefficient 3, higher than propagation by layering coefficient more than 50 times (propagation by layering coefficient is 5), cottage propagation every square metre can produce 400 seedlings simultaneously, 260,000 strains can be bred for every mu, comparatively division propagation, 330/ mu high more than 780 times, comparatively propagation by layering is high more than 470 times.
Embodiment
the following examples can further illustrate the present invention, but do not limit the present invention in any way.
ABT root-inducing powder (be called for short ABT, buy in the Chinese Academy of Forestry) in following examples, plant growth regulator is two gill green plant growth regulators (hereinafter referred to as GGR, buying in the Chinese Academy of Forestry).Carbendazim and tpn are and are purchased.
Embodiment 1,
(1) early-stage preparations:
1.1 conventional methods build green house, and peripheral hardware shading rate is the low-pressure polyethylene sunshade net of 60%; Green house is provided with gateway, is provided with Temperature Humidity Sensor, spraying apparatus, irrigation installation, cistern in it.
Behind ground in 1.2 smooth described green houses, successively lay by the width of every 115cm clean fine sand that a layer thickness is 3cm and thickness is that the cobblestone of 6cm is as cutting bed; The working path of reserved 35cm between cutting bed; Cutting medium loads non-woven fabrics nursery mesh bag, and abundant moistening rear loading cave dish of spraying water, furnishes successively on cutting bed;
Cutting medium selects vermiculite 32kg, perlite 32kg, peat composed of rotten mosses 32kg to mix, and cutting medium loads non-woven fabrics nursery mesh bag after the potassium permanganate spray disinfectant that mass concentration is 0.1%;
1.3 above cutting bed middle lay several fog-spray nozzle, the spacing of adjacent fog-spray nozzle is 1.1m;
(2) the preparation of cuttings:
France Ge Lasi rose Post flowering 45d, choose then raw new slightly, the thick 0.5cm of stem, healthy growth without the plant of damage by disease and insect as the female branch of cuttings; The female branch of cuttings is one section by the branch of every 3 joints, long 10cm before sunrise, tangent plane smooth female branch in clip top and the bottom is as cuttings, female branch only retains goes up 1 joint, 4 compound leaves most, female branch lower cut 2mm place and in angle below the position of bottom bud, upper cut 2mm place and level and smooth above the position of the top bud; Cuttings after cutting is In Shade, and sprays water in time or put into tank and absorb water fresh-keeping stand-by;
(3) grow promotion: female for cuttings branch lower cut position 2cm is immersed growth and promotes, in mixed liquor, to take out after soaking 60min; Growth promotes the composition of mixed liquor: ABT root-inducing powder (hereinafter referred to as ABT) 100mg/L(50kg), two gill green plant growth regulator (hereinafter referred to as GGR) 200mg/L(50kg), the C of 5% concentration
6h
12o
6the aqueous solution (8kg), Cobastab
12parenteral solution 200mg/L(8kg);
(4) cuttage:
Cutting medium central authorities in the dish of cave beat 1.5cm deeply, diameter is the cutting hole of 0.5cm, then promote that the cuttings cuttage of liquid is in hole by having soaked growth, water spray irrigates cutting medium and uses have gentle hands light press immediately, make the lower cut close contact of cutting medium and cuttings, and make cuttings retain 3 joints outward at cutting medium;
(5) the management after cuttage:
5.1 spraying and sterilizations:
Cuttage is sprayed simultaneously, until the blade face of cuttings forms water membrane; Then within every 6 days, use 500 times of mancozeb 50kg respectively, concentration is the carbendazim 50kg of 800 times, and concentration is that 1000 times of tpn 50kg mixed liquors replace blade face sterilization, and night carries out;
5.2 temperature and humidity requirement:
Keep indoor temperature 28 DEG C, air humidity 90%, cutting medium humidity 55%;
5.3 short roots:
Within after Callus formation every 7 days, impose foliage fertilizer once, alternately use 0.4% urea (50kg) and potassium dihydrogen phosphate (50kg) mixed liquor, ABT root-inducing powder and plant growth regulator (GGR), night carries out;
5.4 illumination control:
When the temperature in green house covers described sunshade net higher than when 30 DEG C, all the other time closes;
(6) hardening and transplanting:
After cuttings generally grows side root, carry out hardening according to a conventional method, and within first 6 days, start progressively to cut off the water in transplanting; When root system grows described non-woven fabrics nursery mesh bag, can transplant.
The best results of the present embodiment method.
Embodiment 2
(1) early-stage preparations:
1.1 conventional methods build green house, and peripheral hardware shading rate is the low-pressure polyethylene sunshade net of 75%; Green house is provided with gateway, is provided with Temperature Humidity Sensor, spraying apparatus, irrigation installation, cistern in it.
Behind ground in 1.2 smooth described green houses, successively lay by the width of every 120cm clean fine sand that a layer thickness is 5cm and thickness is that the cobblestone of 8cm is as cutting bed; The working path of reserved 40cm between cutting bed; Cutting medium loads non-woven fabrics nursery mesh bag, and abundant moistening rear loading cave dish of spraying water, furnishes successively on cutting bed;
Cutting medium selects vermiculite 35kg, perlite 35kg, rice hull carbon 40kg mixing, and cutting medium loads non-woven fabrics nursery mesh bag after 200 times of liquid carbendazim spray disinfectants;
1.3 above cutting bed middle lay several fog-spray nozzle, the spacing of adjacent fog-spray nozzle is 1.2m;
(2) the preparation of cuttings:
France Ge Lasi rose Post flowering 60d, choose then raw new slightly, the thick 0.6cm of stem, healthy growth without the plant of damage by disease and insect as the female branch of cuttings; The female branch of cuttings is one section by the branch of every 4 joints, long 12cm before sunrise, tangent plane smooth female branch in clip top and the bottom is as cuttings, female branch only retains goes up 1 joint, 6 compound leaves most, female branch lower cut 3mm place and in angle below the position of bottom bud, upper cut 3mm place and level and smooth above the position of the top bud; Cuttings after cutting is In Shade, and sprays water in time or put into tank and absorb water fresh-keeping stand-by;
(3) grow promotion: female for cuttings branch lower cut position 2cm is immersed growth and promotes, in mixed liquor, to take out after soaking 90min; Growth promotes the composition of mixed liquor: ABT root-inducing powder (hereinafter referred to as ABT) 100mg/L(60kg), two gill green plant growth regulator (hereinafter referred to as GGR) 200mg/L(55kg), the C of 5% concentration
6h
12o
6the aqueous solution (10kg), Alpha Vee-12 200mg/L(10kg);
(4) cuttage:
Cutting medium central authorities in the dish of cave beat 2cm deeply, diameter is the cutting hole of 0.6cm, then promote that the cuttings cuttage of liquid is in hole by having soaked growth, water spray irrigates cutting medium and uses have gentle hands light press immediately, make the lower cut close contact of cutting medium and cuttings, and make cuttings retain 3 joints outward at cutting medium;
(5) the management after cuttage:
5.1 spraying and sterilizations:
Cuttage is sprayed simultaneously, until the blade face of cuttings forms water membrane; Then within every 7 days, use 500 times of mancozebs respectively, the concentration of 55kg is carbendazim, the 55kg concentration of 800 times is that 1000 times of tpn mixed liquors replace blade face sterilization, and night carries out;
5.2 temperature and humidity requirement:
Keep indoor temperature 30 DEG C, air humidity 95%, cutting medium humidity 60%;
5.3 short roots:
Within after Callus formation every 8 days, impose foliage fertilizer once, alternately use 0.4% urea (55%) and potassium dihydrogen phosphate (55%) mixed liquor, ABT root-inducing powder and plant growth regulator (GGR), night carries out;
5.4 illumination control:
When the temperature in green house covers described sunshade net higher than when 30 DEG C, all the other time closes;
(6) hardening and transplanting:
After cuttings generally grows side root, carry out hardening according to a conventional method, and within first 7 days, start progressively to cut off the water in transplanting; When root system grows described non-woven fabrics nursery mesh bag, can transplant.
Embodiment 3
(1) early-stage preparations:
1.1 conventional methods build green house, and peripheral hardware shading rate is the low-pressure polyethylene sunshade net of 50%; Green house is provided with gateway, is provided with Temperature Humidity Sensor, spraying apparatus, irrigation installation, cistern in it.
Behind ground in 1.2 smooth described green houses, successively lay by the width of every 110cm clean fine sand that a layer thickness is 2cm and thickness is that the cobblestone of 4cm is as cutting bed; The working path of reserved 30cmcm between cutting bed; Cutting medium loads non-woven fabrics nursery mesh bag, and abundant moistening rear loading cave dish of spraying water, furnishes successively on cutting bed;
Cutting medium selects vermiculite (30kg), perlite (30kg), the peat composed of rotten mosses or rice hull carbon (35kg) to mix, cutting medium through mass concentration be 0.1% potassium permanganate or 200 times of liquid carbendazim spray disinfectants after load non-woven fabrics nursery mesh bag;
1.3 above cutting bed middle lay several fog-spray nozzle, the spacing of adjacent fog-spray nozzle is 1m;
(2) the preparation of cuttings:
France Ge Lasi rose Post flowering 30d, choose then raw new slightly, the thick 0.4cm of stem, healthy growth without the plant of damage by disease and insect as the female branch of cuttings; The female branch of cuttings is one section by the branch of every 3 joints, long 8cm before sunrise, tangent plane smooth female branch in clip top and the bottom is as cuttings, female branch only retains goes up 1 joint, 2 compound leaves most, female branch lower cut 1mm place and in angle below the position of bottom bud, upper cut 1mm place and level and smooth above the position of the top bud; Cuttings after cutting is In Shade, and sprays water in time or put into tank and absorb water fresh-keeping stand-by;
(3) grow promotion: female for cuttings branch lower cut position lcm is immersed growth and promotes, in mixed liquor, to take out after soaking 60min; Growth promotes the composition of mixed liquor: ABT root-inducing powder (hereinafter referred to as ABT) 100mg/L(45kg), two gill green plant growth regulator (hereinafter referred to as GGR) 200mg/L(45kg), the C of 5% concentration
6h
12o
6the aqueous solution (5kg), Alpha Vee-12 200mg/L(5kg);
(4) cuttage:
Cutting medium central authorities in the dish of cave beat 1cm deeply, diameter is the cutting hole of 0.4cm, then promote that the cuttings cuttage of liquid is in hole by having soaked growth, water spray irrigates cutting medium and uses have gentle hands light press immediately, make the lower cut close contact of cutting medium and cuttings, and make cuttings retain 2 joints outward at cutting medium;
(5) the management after cuttage:
5.1 spraying and sterilizations:
Cuttage is sprayed simultaneously, until the blade face of cuttings forms water membrane; Then within every 5 days, use 500 times of mancozebs respectively, the concentration of 45kg is carbendazim, the 45kg concentration of 800 times is that 1000 times of tpn mixed liquors replace blade face sterilization, and night carries out;
5.2 temperature and humidity requirement:
Keep indoor temperature 25 DEG C, air humidity 80%, cutting medium humidity 50%;
5.3 short roots:
Within after Callus formation every 6 days, impose foliage fertilizer once, alternately use the mixed liquor of 0.4% urea (45kg) and potassium dihydrogen phosphate (45kg), ABT root-inducing powder and plant growth regulator (GGR), night carries out;
5.4 illumination control:
When the temperature in green house covers described sunshade net higher than when 30 DEG C, all the other time closes;
(6) hardening and transplanting:
After cuttings generally grows side root, carry out hardening according to a conventional method, and within first 5 days, start progressively to cut off the water in transplanting; When root system grows described non-woven fabrics nursery mesh bag, can transplant.
Embodiment 4
A kind of French Ge Lasi rose cuttage and seedling culture method, comprises the steps:
(1) early-stage preparations:
1.1 conventional methods build green house, and peripheral hardware shading rate is the low-pressure polyethylene sunshade net of 75%;
Behind ground in 1.2 smooth described green houses, in green house, liftoff 20-30cm place builds the cutting bed that steelframe steel mesh is made; Cutting medium loads non-woven fabrics nursery mesh bag, and abundant moistening rear loading cave dish of spraying water, furnishes successively on cutting bed;
Cutting medium selects vermiculite (30kg), perlite (30kg), the peat composed of rotten mosses or rice hull carbon (35kg) to mix, cutting medium through mass concentration be 0.1% potassium permanganate or 200 times of liquid carbendazim spray disinfectants after load non-woven fabrics nursery mesh bag;
(2) the preparation of cuttings:
France Ge Lasi rose Post flowering 60d, choose then raw new slightly, the thick 0.6cm of stem, healthy growth without the plant of damage by disease and insect as the female branch of cuttings; The female branch of cuttings is by every 4 joints before sunrise, and the branch of long 12cm is one section, and tangent plane smooth female branch in clip top and the bottom is as cuttings, and female branch only retains goes up 1 joint, 6 compound leaves most;
(3) grow promotion: growth is immersed at the lower cut position of female for the cuttings of clip branch and promotes, in mixed liquor, to soak; Growth promotes the composition of mixed liquor: the C of ABT root-inducing powder (hereinafter referred to as ABT) 60kg, two gill green plant growth regulator (hereinafter referred to as GGR) 55kg, concentration
6h
12o
6aqueous solution 10kg, Alpha Vee-12 10kg;
(4) by having soaked growth, cuttage: cutting hole is beaten by the cutting medium central authorities in the dish of cave, then promotes that the cuttings cuttage of liquid is in hole.
(5) the management after cuttage:
5.1 spraying and sterilizations: cuttage is sprayed simultaneously, until the blade face of cuttings forms water membrane; Then to blade face sterilization;
5.2 short roots: impose foliage fertilizer once in after Callus formation every 8 days;
(6) hardening and transplanting:
After cuttings generally grows side root, carry out hardening according to a conventional method, and within first 7 days, start progressively to cut off the water in transplanting; When root system grows described non-woven fabrics nursery mesh bag, can transplant.
Embodiment 5, difference from Example 4 are to increase: laying cutting bed method in early-stage preparations step is successively lay by the width of every 120cm clean fine sand that a layer thickness is 5cm and thickness is that the cobblestone of 8cm is as cutting bed; The working path of reserved 40cm between cutting bed; Cutting medium through mass concentration be 0.1% potassium permanganate or 200 times of liquid carbendazim spray disinfectants after load non-woven fabrics nursery mesh bag; Above cutting bed, several fog-spray nozzle is laid in middle, and the spacing of adjacent fog-spray nozzle is 1.2m.
Embodiment 6, difference from Example 5 are to increase: growth promotes concentration following ABT root-inducing powder 100mg/L, the plant growth regulator 200mg/L of mixed liquor, the C of 5% concentration
6h
12o
6the aqueous solution, Alpha Vee-12 200mg/L.
Embodiment 7, difference from Example 6 are to increase: female branch lower cut 3mm place and in angle, upper cut is 3mm place above the position of the top bud below the position of bottom bud in the preparation of described step (2) cuttings.
Embodiment 8, difference from Example 7 are to increase: (3) described step grows in promotion and immersed in growth promotion mixed liquor by female for cuttings branch lower cut position 2cm, soaks 60min.
Embodiment 9, difference from Example 8 are to increase: described step (4) in concrete grammar be cutting medium central authorities beat 2cm deeply, diameter is the cutting hole of 0.6cm, then by cuttings cuttage in hole, water spray irrigates cutting medium and uses have gentle hands light press immediately, and makes slotting rear described cutting medium retain 3 joints outward.
Embodiment 10, difference from Example 9 are to increase: the method for disinfection in the management of described step (5) after cuttage is use 500 times of mancozebs respectively in every 7 days, 800 times of carbendazim and 1000 times of tpn mixed liquors replace blade face sterilization, carbendazim 50kg, tpn 50kg, mixed liquor replaces blade face sterilization, and night carries out.
Embodiment 11, difference from Example 10 are to increase: be that temperature 25-30 DEG C, air humidity 80-95%, cutting medium humidity is 50-60% to temperature and humidity requirement in the management of described step (5) after cuttage.
Embodiment 12, be to increase with embodiment 11 difference: the short method for root in the management of described step (5) after cuttage is after Callus formation, alternately use 0.4% urea and potassium dihydrogen phosphate mixed liquor, ABT root-inducing powder, plant growth regulator, night carries out; The mass content of urea is 45%-55%, and the mass content of potassium dihydrogen phosphate is 45%-55%.
Embodiment 13, be to increase with embodiment 12 difference: also carry out illumination control in the management of described step (5) after cuttage, when the temperature in green house is higher than 30 DEG C, cover described sunshade net, all the other time is closed.
" seedling growth test "
2010-2014 years, successively seedling raising manners is tested the impact of rooting rate, the impact of different substrates on rooting rate the impact of rooting rate, HORMONE TREATMENT the impact of rooting rate, cuttings clip, existing that description of test is as follows.
1. seedling raising manners is on the impact of rooting rate
In July, 2010, August, carry out press strip experiment; In June, 2010, July, intermittent spraying equipment is utilized to carry out epicormic branch cuttage; In March, 2010, November, in greenhouse by solar heat and land for growing field crops plastic canopy, carry out cuttage; Arrange three sample prescriptions altogether, the strain of every sample prescription 250 is tested in cuttage, and the strain of every sample prescription 100 tested by press strip, adds up rooting rate, compare 3 kinds of modes to the impact of rooting rate under the prerequisite of not carrying out HORMONE TREATMENT, unified matrix.
From table 1, table 2, when without somatotropin process, rose cuttage rooting rate is lower than 10%, and the rooting rate of press strip and epicormic branch cuttage is suitable, but the shortcoming of press strip to be reproduction coefficient low, every mu of ground about nursery 500 to 600 strain; Breeding cycle is long, can transplant about 4-5 month (then February November to next year) from press strip to last seedling; Complex operation poor controllability, layering seedling growth wants gradation to carry out, and at substantial is artificial.And cottage propagation coefficient is high, every strain 3 years above plant of life can obtain cuttage female branch 40-50 strain, and the female branch of each cuttage can be cut into 3-4 cuttings, and cuttage every square can produce 350 seedlings, within about 40 days, can transplant.
2. cuttings clip is on the impact of rooting rate
In June, 2011, compare test, explore cuttings clip and stay leaf on the impact of rooting rate.
Table 3 is known, and upper clip is level and smooth, and lower clip angle is the best mode of rose cuttage and seedling culture, and upper clip smoothly can reduce transpiration, and lower clip angle can increase crop Callus formation area, and the moisture of absorption base and nutrient better.
3. HORMONE TREATMENT is on the impact of rooting rate
The test of 3.1 first times
In June, 2012, adopt three level four way crossover experimental design (see table 4), first time explores growth hormone to the impact of cuttage, and statistics rooting rate, carries out data analysis with Excel and DPS software.
From table 5,6,4 factors (hormone, concentration, processing time, matrix) on rose cutting plantation to affect difference extremely remarkable; Table 7 is known, the rooting percent of ABT and IBA process is higher than NAA process, the combination that rooting rate is the highest is 100mg/kg ABT process 1 h cuttage in vermiculite, in 4 single-matrixes, in vermiculite, cutting plantation is the highest, in river sand, cutting plantation is minimum, and hormone low concentration soaks the many of Be very effective more quickly dipped than high concentration for a long time.
3.2 second time tests
In June, 2013, adopt three level four way crossover experimental design (see table 8), the impact of further Optimal Growing element on cuttage, statistics rooting rate, carries out data analysis with Excel and DPS software.
From table 9,10,4 factors (hormone, concentration, processing time, matrix) on rose cutting plantation to affect difference extremely remarkable, this is with experiment conclusion is consistent for the first time; As shown in Table 11, the rooting percent of ABT and GGR process is higher than the root sun, and the combination that rooting rate is the highest is 200mg/kg GGR process 1 .5h cuttage in vermiculite, and in 4 single-matrixes, in vermiculite, cutting plantation is the highest, and in river sand, cutting plantation is minimum.
The experiment of 3.3 third times
In August, 2013, carry out HORMONE TREATMENT contrast experiment, add glucose injection and vitamin b12 parenteral solution factor of influence simultaneously, statistics rooting rate.
From table 5,9,12, the rooting percent of ABT and GGR mixed processing is higher than single HORMONE TREATMENT, ABT 100mg/kg and GGR 200mg/kg solution 1:1 mix that to soak 1-1.5h effect best, add glucose injection and vitamin b12 parenteral solution can improve rose cutting plantation.
4. different substrates is on the impact of rooting rate
In June, 2014, above-mentioned twice experimental basis is explored matrix further on the impact of rooting rate, take into full account known river sand, the perlite bad as single-matrix effect, carry out contrasting (see table 13) in the peat composed of rotten mosses, perlite+vermiculite (1:1), perlite+peat composed of rotten mosses (1:1), the peat composed of rotten mosses+vermiculite (1:1), perlite+vermiculite+peat composed of rotten mosses (1:1:1) 5 kinds of matrix, statistics rooting rate is also made comparisons with given data.
From table 5,9,12,13, in single-matrix, vermiculite rooting rate is the highest, and mixed-matrix rooting rate is greater than single-matrix, and optimum substrate combination is perlite+vermiculite+peat composed of rotten mosses (1:1:1).
Claims (10)
1. a French Ge Lasi rose cuttage and seedling culture method, is characterized in that it comprises the steps:
(1) early-stage preparations:
1.1 conventional methods build green house, and peripheral hardware shading rate is the low-pressure polyethylene sunshade net of 50%-75%;
Behind ground in 1.2 smooth green houses, lay cutting bed; Cutting medium loads non-woven fabrics nursery mesh bag, and abundant moistening rear loading cave dish of spraying water, furnishes successively on cutting bed;
Described cutting medium is made up of the raw material of following weight percent, vermiculite 30-35%, perlite 30-35%, the peat composed of rotten mosses or rice hull carbon 35-40%;
(2) the preparation of cuttings:
France Ge Lasi rose Post flowering 30-60d, choose then raw new slightly, the thick 0.4-0.6cm of stem, healthy growth without the plant of damage by disease and insect as the female branch of cuttings; The female branch of cuttings is by every 3-4 joints before sunrise, and the branch of long 8-12cm is one section, and tangent plane smooth female branch in clip top and the bottom is as cuttings, and female branch only retains goes up 1 joint, 2-6 compound leaves most;
(3) grow promotion: growth is immersed at the lower cut position of female for the cuttings of clip branch and promotes, in mixed liquor, to soak; Growth promotes that mixed liquor is made up of the raw material of following weight parts: ABT root-inducing powder 45-60 part, plant growth regulator 45-55 part, C
6h
12o
6aqueous solution 5-10 part, Cobastab
12parenteral solution 5-10 part;
(4) by having soaked growth, cuttage: cutting hole is beaten by the cutting medium central authorities in the dish of cave, then promotes that the cuttings cuttage of liquid is in hole;
(5) the management after cuttage:
5.1 spraying and sterilizations: cuttage is sprayed simultaneously, until the blade face of cuttings forms water membrane; Then to blade face sterilization;
5.2 short roots: impose foliage fertilizer once in after Callus formation every 6-8 days;
(6) hardening and transplanting:
After cuttings generally grows side root, carry out hardening according to a conventional method, and 5-7 days before transplanting start progressively to cut off the water; When root system grows described non-woven fabrics nursery mesh bag, can transplant.
2. one according to claim 1 French Ge Lasi rose cuttage and seedling culture method, is characterized in that: (1) described step lays cutting bed method in early-stage preparations is successively lay by the width of every 110-120cm clean fine sand that a layer thickness is 2-5cm and thickness is that the cobblestone of 4-8cm is as cutting bed; The working path of reserved 30cm-40cm between cutting bed; Cutting medium through mass concentration be 0.1% potassium permanganate or 200 times of liquid carbendazim spray disinfectants after load non-woven fabrics nursery mesh bag; Above cutting bed, several fog-spray nozzle is laid in middle, and the spacing of adjacent fog-spray nozzle is 1-1.2m.
3. one according to claim 1 and 2 French Ge Lasi rose cuttage and seedling culture method, is characterized in that: growth promotes that the concentration of each raw material in mixed liquor is as follows, ABT root-inducing powder 100mg/L, plant growth regulator 200mg/L, C
6h
12o
6aqueous solution mass content 5%, Alpha Vee-12 200mg/L.
4. one according to claim 3 French Ge Lasi rose cuttage and seedling culture method, it is characterized in that: female branch lower cut 1-3mm place and in angle, upper cut is 1-3mm place above the position of the top bud below the position of bottom bud in the preparation of described step (2) cuttings.
5. one according to claim 4 French Ge Lasi rose cuttage and seedling culture method, is characterized in that: (3) described step grows in promotion and immersed in growth promotion mixed liquor by female for cuttings branch lower cut position l-2cm, soaks 60-90min.
6. one according to claim 5 French Ge Lasi rose cuttage and seedling culture method, it is characterized in that: described step (4) in concrete grammar be cutting medium central authorities make a call to 1-2cm deeply, diameter is the cutting hole of 0.4-0.6cm, then by cuttings cuttage in hole, water spray irrigates cutting medium and uses have gentle hands light press immediately, and makes slotting rear described cutting medium retain 2-3 joints outward.
7. one according to claim 6 French Ge Lasi rose cuttage and seedling culture method, it is characterized in that: the method for disinfection in the management of described step (5) after cuttage is use 500 times of mancozebs respectively in every 5-7 days, 800 times of carbendazim and 1000 times of tpn mixed liquors replace blade face sterilization, the mass content of carbendazim is 45%-55%, the mass content of tpn is 45%-55%, mixed liquor replaces blade face sterilization, and night carries out.
8. one according to claim 7 French Ge Lasi rose cuttage and seedling culture method, is characterized in that: be that temperature 25-30 DEG C, air humidity 80-95%, cutting medium humidity is 50-60% to temperature and humidity requirement in the management of described step (5) after cuttage.
9. one according to claim 8 French Ge Lasi rose cuttage and seedling culture method, it is characterized in that: the short method for root in the management of described step (5) after cuttage is after Callus formation, alternately use 0.4% urea and potassium dihydrogen phosphate mixed liquor, ABT root-inducing powder, plant growth regulator, night carries out; The mass content of urea is 45%-55%, and the mass content of potassium dihydrogen phosphate is 45%-55%.
10. one according to claim 9 French Ge Lasi rose cuttage and seedling culture method, is characterized in that: carry out illumination control in the management of described step (5) after cuttage, and when the temperature in green house is higher than 30 DEG C, covered with sunshade net, all the other time closes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510335818.3A CN104904481B (en) | 2015-06-17 | 2015-06-17 | A kind of French Ge Lasi roses cuttage and seedling culture method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510335818.3A CN104904481B (en) | 2015-06-17 | 2015-06-17 | A kind of French Ge Lasi roses cuttage and seedling culture method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104904481A true CN104904481A (en) | 2015-09-16 |
| CN104904481B CN104904481B (en) | 2017-11-14 |
Family
ID=54074279
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510335818.3A Active CN104904481B (en) | 2015-06-17 | 2015-06-17 | A kind of French Ge Lasi roses cuttage and seedling culture method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104904481B (en) |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494065A (en) * | 2015-12-30 | 2016-04-20 | 湖南农业大学 | Method for carrying out cutting seedling by Rosa multiflora Thunb seedling clods |
| CN105519417A (en) * | 2015-12-30 | 2016-04-27 | 湖南农业大学 | Method for raising seedlings in plugs by cutting |
| CN105557430A (en) * | 2015-12-30 | 2016-05-11 | 湖南农业大学 | Cutting seedling method for seedling block of rosa chinensis |
| CN105706820A (en) * | 2016-02-02 | 2016-06-29 | 江汉大学 | Oriental cherry cutting propagation method |
| CN105850443A (en) * | 2016-04-20 | 2016-08-17 | 李从喜 | Planting method of Yunnan red rose |
| CN106386246A (en) * | 2016-08-31 | 2017-02-15 | 江苏沿江地区农业科学研究所 | Hardwood branch inverted cuttage method, cuttage device and seedling growing container |
| CN106900473A (en) * | 2017-04-27 | 2017-06-30 | 东营黄蓝知识产权运营管理有限公司 | Europe Lee's epicormic branch cutting method |
| CN107006353A (en) * | 2017-03-30 | 2017-08-04 | 东莞市中实创半导体照明有限公司 | A kind of rose cuttage breeding method and a kind of rose indoor cultivation method for Ganoderma |
| CN107098475A (en) * | 2017-05-03 | 2017-08-29 | 东营黄蓝知识产权运营管理有限公司 | The method that strand Na Wu river courses are administered using watermifoil |
| CN107278557A (en) * | 2017-06-14 | 2017-10-24 | 源涌生物高科技有限公司 | A kind of method for culturing seedlings of rose |
| CN107371674A (en) * | 2017-06-22 | 2017-11-24 | 兰溪市奥而特农业科技有限公司 | A kind of Rapid Rooting method of rose branch |
| CN107517765A (en) * | 2017-08-21 | 2017-12-29 | 黑龙江省科学院自然与生态研究所 | Lonicera edulis cuttage propagation method |
| CN107581044A (en) * | 2017-11-14 | 2018-01-16 | 佴辉 | Poinsettia cuttage fast breeding method |
| CN108271645A (en) * | 2018-01-18 | 2018-07-13 | 广东华辰玫瑰生物科技有限公司 | A kind of method of rose flower seedling summer cutting propagation |
| CN109089814A (en) * | 2018-06-14 | 2018-12-28 | 云南丰德生物科技有限公司 | A kind of rose cuttage breeding method |
| CN111758405A (en) * | 2020-07-30 | 2020-10-13 | 山东华玫生物科技有限公司 | Full-automatic neon spraying hole tray cutting seedling method for purple roses |
| CN113475250A (en) * | 2021-07-02 | 2021-10-08 | 柒久园艺科技(北京)有限公司 | Rose cuttage breeding method |
| CN114342776A (en) * | 2022-01-05 | 2022-04-15 | 新疆维吾尔自治区阿克苏地区林业技术推广服务中心 | Edible rose cuttage substrate and edible rose cuttage method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102612971A (en) * | 2012-04-10 | 2012-08-01 | 西昌昌泰香料有限责任公司 | Rose cutting propagation method |
| CN103650839A (en) * | 2013-10-25 | 2014-03-26 | 安徽环绿生态农业投资发展有限公司 | Cutting seedling-raising method of rose tender branches |
| CN103891509A (en) * | 2014-03-28 | 2014-07-02 | 兰州市农业科技研究推广中心 | Cuttage of Chinese Kushui rose |
| CN103918535A (en) * | 2014-04-28 | 2014-07-16 | 四川银谷玫瑰科技有限公司 | Method for culturing seedling of Damascus rose |
| CN103960006A (en) * | 2013-02-06 | 2014-08-06 | 霍城县科发科技风险投资有限责任公司 | Cuttage method of rose |
-
2015
- 2015-06-17 CN CN201510335818.3A patent/CN104904481B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102612971A (en) * | 2012-04-10 | 2012-08-01 | 西昌昌泰香料有限责任公司 | Rose cutting propagation method |
| CN103960006A (en) * | 2013-02-06 | 2014-08-06 | 霍城县科发科技风险投资有限责任公司 | Cuttage method of rose |
| CN103650839A (en) * | 2013-10-25 | 2014-03-26 | 安徽环绿生态农业投资发展有限公司 | Cutting seedling-raising method of rose tender branches |
| CN103891509A (en) * | 2014-03-28 | 2014-07-02 | 兰州市农业科技研究推广中心 | Cuttage of Chinese Kushui rose |
| CN103918535A (en) * | 2014-04-28 | 2014-07-16 | 四川银谷玫瑰科技有限公司 | Method for culturing seedling of Damascus rose |
Non-Patent Citations (3)
| Title |
|---|
| 王新刚等: "不同品种玫瑰扦插技术研究", 《北方园艺》 * |
| 陈火英等: "《种子种苗学》", 31 August 2011, 上海交通大学出版社 * |
| 陈红武等: "《花卉基本生产新技术》", 31 January 2005, 西北农林科技大学出版社 * |
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519417A (en) * | 2015-12-30 | 2016-04-27 | 湖南农业大学 | Method for raising seedlings in plugs by cutting |
| CN105557430A (en) * | 2015-12-30 | 2016-05-11 | 湖南农业大学 | Cutting seedling method for seedling block of rosa chinensis |
| CN105494065A (en) * | 2015-12-30 | 2016-04-20 | 湖南农业大学 | Method for carrying out cutting seedling by Rosa multiflora Thunb seedling clods |
| CN105706820A (en) * | 2016-02-02 | 2016-06-29 | 江汉大学 | Oriental cherry cutting propagation method |
| CN105850443A (en) * | 2016-04-20 | 2016-08-17 | 李从喜 | Planting method of Yunnan red rose |
| CN106386246B (en) * | 2016-08-31 | 2020-01-10 | 江苏沿江地区农业科学研究所 | Inverted cuttage method and device for hard wood branches and seedling raising container |
| CN106386246A (en) * | 2016-08-31 | 2017-02-15 | 江苏沿江地区农业科学研究所 | Hardwood branch inverted cuttage method, cuttage device and seedling growing container |
| CN107006353A (en) * | 2017-03-30 | 2017-08-04 | 东莞市中实创半导体照明有限公司 | A kind of rose cuttage breeding method and a kind of rose indoor cultivation method for Ganoderma |
| CN106900473A (en) * | 2017-04-27 | 2017-06-30 | 东营黄蓝知识产权运营管理有限公司 | Europe Lee's epicormic branch cutting method |
| CN107098475A (en) * | 2017-05-03 | 2017-08-29 | 东营黄蓝知识产权运营管理有限公司 | The method that strand Na Wu river courses are administered using watermifoil |
| CN107278557A (en) * | 2017-06-14 | 2017-10-24 | 源涌生物高科技有限公司 | A kind of method for culturing seedlings of rose |
| CN107371674A (en) * | 2017-06-22 | 2017-11-24 | 兰溪市奥而特农业科技有限公司 | A kind of Rapid Rooting method of rose branch |
| CN107517765A (en) * | 2017-08-21 | 2017-12-29 | 黑龙江省科学院自然与生态研究所 | Lonicera edulis cuttage propagation method |
| CN107581044A (en) * | 2017-11-14 | 2018-01-16 | 佴辉 | Poinsettia cuttage fast breeding method |
| CN108271645A (en) * | 2018-01-18 | 2018-07-13 | 广东华辰玫瑰生物科技有限公司 | A kind of method of rose flower seedling summer cutting propagation |
| CN109089814A (en) * | 2018-06-14 | 2018-12-28 | 云南丰德生物科技有限公司 | A kind of rose cuttage breeding method |
| CN111758405A (en) * | 2020-07-30 | 2020-10-13 | 山东华玫生物科技有限公司 | Full-automatic neon spraying hole tray cutting seedling method for purple roses |
| CN113475250A (en) * | 2021-07-02 | 2021-10-08 | 柒久园艺科技(北京)有限公司 | Rose cuttage breeding method |
| CN114342776A (en) * | 2022-01-05 | 2022-04-15 | 新疆维吾尔自治区阿克苏地区林业技术推广服务中心 | Edible rose cuttage substrate and edible rose cuttage method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104904481B (en) | 2017-11-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104904481A (en) | Cutting seedling-culturing method for France Grasse roses | |
| CN103340068B (en) | Undergrowth cultivation medium and cultivation method for anoectochilus formosanus | |
| CN103004445B (en) | Method for directly seedling dendrobe seedlings by using plastic greenhouse | |
| CN101743879B (en) | Industrialized grape matrix cultivating method | |
| CN102511258B (en) | Container seedling-raising method for greenhouse of Chinese wolfberry | |
| CN102197776B (en) | Novel technology for twice production of grapes in one year | |
| CN101491188B (en) | Summer mulberry garden-building method | |
| CN103430749B (en) | Seedling growing method for promoting fast rooting of plant cutting slips under high-temperature and high-humidity conditions | |
| CN104396683A (en) | Sunlight greenhouse planting technology of seedless grape | |
| CN103548555B (en) | Greenhouse Chinese toon is gathered implantation methods throughout the year | |
| CN105028089A (en) | Hard-wood cutting seedling method for Lagerstroemia indica Dynamite | |
| CN105123425A (en) | Facility cultivation method for grapes | |
| CN102172190A (en) | Cultivation method of apple bonsai realizing current-year fruiting and market-selling in Spring Festival | |
| CN104145692A (en) | Method for cutting crape myrtle seedlings on seedbed | |
| CN104025830A (en) | Greenhouse cherry cultivation method | |
| CN104521705A (en) | Cultivating and conserving method for facility strawberries | |
| CN102349430B (en) | Method for breeding seedlings of grapes by using containers | |
| CN104542223A (en) | Full-illumination soilless cuttage propagation technique | |
| CN105028103A (en) | Sunlight greenhouse trough cultivation method for peach plants | |
| CN104285748A (en) | Method for improving survival rate of pachira macrocarpa seed seedling | |
| CN105409531A (en) | Disease and insect pest occurrence reducing lotus root planting method for lotus root ponds | |
| CN107318421A (en) | The cuttage breeding method of summer high temperature area Japan cuckoo | |
| CN109197544B (en) | Dwarfing method of aquatic canna | |
| CN107853046A (en) | A kind of wet-land pine tree industrial cuttage method for culturing seedlings | |
| CN104094819A (en) | Factory-like plug seedling method of dendrobium officinale |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |