CN104849468B - 检测血清糖蛋白岩藻糖指数的化学发光蛋白芯片、试剂盒及检测方法 - Google Patents
检测血清糖蛋白岩藻糖指数的化学发光蛋白芯片、试剂盒及检测方法 Download PDFInfo
- Publication number
- CN104849468B CN104849468B CN201510058591.2A CN201510058591A CN104849468B CN 104849468 B CN104849468 B CN 104849468B CN 201510058591 A CN201510058591 A CN 201510058591A CN 104849468 B CN104849468 B CN 104849468B
- Authority
- CN
- China
- Prior art keywords
- detection
- afp
- spot
- serum
- protein chip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 102
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 34
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 34
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 title claims abstract description 23
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 title claims abstract description 23
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 title claims abstract description 23
- 210000002966 serum Anatomy 0.000 claims abstract description 75
- 108010026331 alpha-Fetoproteins Proteins 0.000 claims abstract description 33
- 239000011521 glass Substances 0.000 claims abstract description 5
- 239000011159 matrix material Substances 0.000 claims abstract description 5
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims abstract description 4
- 229940098773 bovine serum albumin Drugs 0.000 claims abstract description 4
- 239000000463 material Substances 0.000 claims abstract description 3
- 102100023635 Alpha-fetoprotein Human genes 0.000 claims abstract 10
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 32
- 230000033581 fucosylation Effects 0.000 claims description 26
- 229960002685 biotin Drugs 0.000 claims description 17
- 235000020958 biotin Nutrition 0.000 claims description 17
- 239000011616 biotin Substances 0.000 claims description 17
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 11
- 239000003153 chemical reaction reagent Substances 0.000 claims description 10
- 108090001008 Avidin Proteins 0.000 claims description 9
- UQLDLKMNUJERMK-UHFFFAOYSA-L di(octadecanoyloxy)lead Chemical compound [Pb+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O UQLDLKMNUJERMK-UHFFFAOYSA-L 0.000 claims description 9
- 238000002372 labelling Methods 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 9
- 239000000758 substrate Substances 0.000 claims description 8
- 238000004020 luminiscence type Methods 0.000 claims description 5
- 241000196324 Embryophyta Species 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 32
- 238000005516 engineering process Methods 0.000 abstract description 7
- 230000035945 sensitivity Effects 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000002331 protein detection Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 59
- 102000013529 alpha-Fetoproteins Human genes 0.000 description 28
- 201000007270 liver cancer Diseases 0.000 description 26
- 208000014018 liver neoplasm Diseases 0.000 description 26
- 238000012360 testing method Methods 0.000 description 20
- 150000001875 compounds Chemical class 0.000 description 10
- 239000012895 dilution Substances 0.000 description 9
- 238000010790 dilution Methods 0.000 description 9
- 238000002965 ELISA Methods 0.000 description 8
- 239000000427 antigen Substances 0.000 description 8
- 102000036639 antigens Human genes 0.000 description 8
- 108091007433 antigens Proteins 0.000 description 8
- 230000008569 process Effects 0.000 description 7
- 238000012417 linear regression Methods 0.000 description 6
- 230000009871 nonspecific binding Effects 0.000 description 6
- 239000012925 reference material Substances 0.000 description 6
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 description 5
- 244000046052 Phaseolus vulgaris Species 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 238000011088 calibration curve Methods 0.000 description 5
- 235000005489 dwarf bean Nutrition 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 241000195493 Cryptophyta Species 0.000 description 3
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 3
- 150000001720 carbohydrates Chemical group 0.000 description 3
- 230000004907 flux Effects 0.000 description 3
- 206010073071 hepatocellular carcinoma Diseases 0.000 description 3
- 208000019423 liver disease Diseases 0.000 description 3
- 239000011435 rock Substances 0.000 description 3
- 206010073069 Hepatic cancer Diseases 0.000 description 2
- 230000027455 binding Effects 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 2
- 125000003147 glycosyl group Chemical group 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 238000004393 prognosis Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 101710153593 Albumin A Proteins 0.000 description 1
- 101710085718 Defensin-like protein 3 Proteins 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 101710094902 Legumin Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000007882 cirrhosis Effects 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- NEKNNCABDXGBEN-UHFFFAOYSA-L disodium;4-(4-chloro-2-methylphenoxy)butanoate;4-(2,4-dichlorophenoxy)butanoate Chemical compound [Na+].[Na+].CC1=CC(Cl)=CC=C1OCCCC([O-])=O.[O-]C(=O)CCCOC1=CC=C(Cl)C=C1Cl NEKNNCABDXGBEN-UHFFFAOYSA-L 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 125000002446 fucosyl group Chemical group C1([C@@H](O)[C@H](O)[C@H](O)[C@@H](O1)C)* 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 150000002402 hexoses Chemical class 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000000760 immunoelectrophoresis Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000000504 luminescence detection Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 102000013415 peroxidase activity proteins Human genes 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 239000003726 plant lectin Substances 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000013139 quantization Methods 0.000 description 1
- 239000012898 sample dilution Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
- G01N33/57488—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds identifable in body fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57438—Specifically defined cancers of liver, pancreas or kidney
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B01J2219/00527—Sheets
- B01J2219/00533—Sheets essentially rectangular
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00614—Delimitation of the attachment areas
- B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
- B01J2219/00662—Two-dimensional arrays within two-dimensional arrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/0068—Means for controlling the apparatus of the process
- B01J2219/00693—Means for quality control
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00725—Peptides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/415—Assays involving biological materials from specific organisms or of a specific nature from plants
- G01N2333/42—Lectins, e.g. concanavalin, phytohaemagglutinin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/471—Pregnancy proteins, e.g. placenta proteins, alpha-feto-protein, pregnancy specific beta glycoprotein
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
- G01N2440/38—Post-translational modifications [PTMs] in chemical analysis of biological material addition of carbohydrates, e.g. glycosylation, glycation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/08—Hepato-biliairy disorders other than hepatitis
- G01N2800/085—Liver diseases, e.g. portal hypertension, fibrosis, cirrhosis, bilirubin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/50—Determining the risk of developing a disease
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/70—Mechanisms involved in disease identification
- G01N2800/7023—(Hyper)proliferation
- G01N2800/7028—Cancer
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Plasma & Fusion (AREA)
- Gastroenterology & Hepatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Description
Claims (8)
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510058591.2A CN104849468B (zh) | 2014-08-05 | 2015-02-04 | 检测血清糖蛋白岩藻糖指数的化学发光蛋白芯片、试剂盒及检测方法 |
US14/622,259 US20160223556A1 (en) | 2014-08-05 | 2015-02-13 | Chemiluminescent Protein Chip, Method and Kit for Detecting Seroglycoid Fucosylation Index |
SE1750227A SE1750227A1 (en) | 2014-08-05 | 2015-07-17 | Chemiluminescent Protein Chip, KIT and Method for DetectingSeroglycoid Fucose Index |
GB1703351.5A GB2548978B (en) | 2014-08-05 | 2015-07-17 | A chemiluminescent protein chip seroglycoid fucosylations index assay comprising AFP specific antibodies and Lens culinaris lectin |
KR1020177006041A KR20170040318A (ko) | 2014-08-05 | 2015-07-17 | 혈청 당단백질 푸코오스 지수를 검측하는 화학 발광 단백질 칩, 키트 및 이의 검측방법 |
KR1020187009587A KR101914673B1 (ko) | 2014-08-05 | 2015-07-17 | 혈청 당단백질 푸코오스 지수를 검측하는 화학 발광 단백질 칩, 키트 및 이의 검측방법 |
PCT/CN2015/084356 WO2016019797A1 (zh) | 2014-08-05 | 2015-07-17 | 检测血清糖蛋白岩藻糖指数的化学发光蛋白芯片、试剂盒及检测方法 |
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410381461 | 2014-08-05 | ||
CN2014103814618 | 2014-08-05 | ||
CN2014107726654 | 2014-12-12 | ||
CN201410772665.4A CN104678103A (zh) | 2014-08-05 | 2014-12-12 | 检测血清糖蛋白岩藻糖指数的化学发光蛋白芯片、试剂盒及检测方法 |
CN201510058591.2A CN104849468B (zh) | 2014-08-05 | 2015-02-04 | 检测血清糖蛋白岩藻糖指数的化学发光蛋白芯片、试剂盒及检测方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104849468A CN104849468A (zh) | 2015-08-19 |
CN104849468B true CN104849468B (zh) | 2018-02-13 |
Family
ID=53313448
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410772665.4A Pending CN104678103A (zh) | 2014-08-05 | 2014-12-12 | 检测血清糖蛋白岩藻糖指数的化学发光蛋白芯片、试剂盒及检测方法 |
CN201510058591.2A Expired - Fee Related CN104849468B (zh) | 2014-08-05 | 2015-02-04 | 检测血清糖蛋白岩藻糖指数的化学发光蛋白芯片、试剂盒及检测方法 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410772665.4A Pending CN104678103A (zh) | 2014-08-05 | 2014-12-12 | 检测血清糖蛋白岩藻糖指数的化学发光蛋白芯片、试剂盒及检测方法 |
Country Status (9)
Country | Link |
---|---|
US (1) | US20160223556A1 (zh) |
JP (1) | JP6082767B2 (zh) |
KR (2) | KR101914673B1 (zh) |
CN (2) | CN104678103A (zh) |
AU (1) | AU2016101432A4 (zh) |
CA (1) | CA2882151C (zh) |
GB (1) | GB2548978B (zh) |
SE (1) | SE1750227A1 (zh) |
WO (1) | WO2016019797A1 (zh) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102166711A (zh) * | 2010-02-26 | 2011-08-31 | 昆山巨仲电子有限公司 | 散热器的制作方法及其散热器 |
CN104678103A (zh) * | 2014-08-05 | 2015-06-03 | 首都医科大学附属北京佑安医院 | 检测血清糖蛋白岩藻糖指数的化学发光蛋白芯片、试剂盒及检测方法 |
CN105785043B (zh) * | 2016-04-06 | 2018-02-02 | 上海良润生物医药科技有限公司 | 用于定量检测afp‑l3%的试剂盒 |
JP6935184B2 (ja) * | 2016-05-31 | 2021-09-15 | シスメックス株式会社 | 糖ペプチドと反応するモノクローナル抗体およびその用途 |
CN106198998A (zh) * | 2016-06-30 | 2016-12-07 | 深圳市亚辉龙生物科技股份有限公司 | 人甲胎蛋白异质体3化学发光免疫检测试剂盒及其制备方法 |
CN107727864A (zh) * | 2016-07-01 | 2018-02-23 | 首都医科大学附属北京佑安医院 | 一种检测血清中异常脱羧凝血酶原的蛋白芯片、试剂盒及其制备方法 |
CN106248959B (zh) * | 2016-07-21 | 2019-01-25 | 首都医科大学附属北京佑安医院 | 一种检测血清岩藻糖蛋白的免疫渗滤方法及免疫渗滤装置 |
CN107748261A (zh) * | 2017-06-30 | 2018-03-02 | 首都医科大学附属北京佑安医院 | 一种检测血清中异常脱羧凝血酶原的蛋白芯片、试剂盒及其制备方法 |
CN107525937A (zh) * | 2017-08-25 | 2017-12-29 | 苏州优函信息科技有限公司 | 基于上转换发光标记物、蛋白芯片及检测方法 |
CN108872594A (zh) * | 2018-07-05 | 2018-11-23 | 潍坊市康华生物技术有限公司 | 一种甲胎蛋白检测试剂盒及其制备方法 |
CN111665235A (zh) * | 2019-03-08 | 2020-09-15 | 上海索昕生物科技有限公司 | 一种化学发光微阵列芯片及其应用 |
CN116162538B (zh) * | 2022-12-16 | 2024-01-26 | 中国科学院苏州生物医学工程技术研究所 | 一种同时检测蛋白和rna的微流控芯片及试剂盒 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN2613759Y (zh) * | 2003-03-31 | 2004-04-28 | 穆海东 | 一种蛋白质芯片 |
CN101603966A (zh) * | 2008-06-12 | 2009-12-16 | 上海裕隆生物科技有限公司 | 一种男性多肿瘤标志物检测蛋白芯片及其试剂盒 |
CN102695716A (zh) * | 2009-07-14 | 2012-09-26 | 独立行政法人产业技术综合研究所 | 肝病病情指标糖链标记物 |
CN103823058A (zh) * | 2014-02-27 | 2014-05-28 | 首都医科大学附属北京佑安医院 | 血清中抗原类蛋白的化学发光蛋白芯片方法和试剂盒 |
Family Cites Families (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0718875B2 (ja) * | 1987-06-19 | 1995-03-06 | ヤマサ醤油株式会社 | 血中または体液中の微量物質含有量の測定法 |
JPH07325083A (ja) * | 1994-05-31 | 1995-12-12 | Nakarai Tesuku Kk | 糖タンパク質の特定糖鎖割合の測定方法 |
DE19806185C2 (de) * | 1998-02-02 | 1999-11-18 | Biogenes Gmbh | Immunoassay und Testkit zur Bestimmung von fucosyliertem Protein in einer biologischen Probe |
JP3833955B2 (ja) * | 2002-03-27 | 2006-10-18 | 株式会社東芝 | 光導波路型プロテインチップおよびプロテイン検出装置 |
EP1495323A2 (en) * | 2002-04-05 | 2005-01-12 | University Of Georgia Research Foundation, Inc. | Method for cleaving and deglycosylating antibodies to promote ligand binding |
GB0212391D0 (en) * | 2002-05-29 | 2002-07-10 | Axis Shield Asa | Assay |
GB2404734A (en) * | 2003-08-05 | 2005-02-09 | Secr Defence | A method of screening a sample for abnormally glycosylated and/or expressed proteins |
EP1710581B1 (en) * | 2003-12-25 | 2014-03-19 | National Institute of Advanced Industrial Science and Technology | Method for analyzing interactions between proteins and sugar chains |
EP1870709A4 (en) * | 2005-03-25 | 2011-09-28 | Ngk Insulators Ltd | PROBE ARRAY AND METHOD FOR THE PRODUCTION THEREOF |
JP2007132866A (ja) * | 2005-11-11 | 2007-05-31 | Toyota Central Res & Dev Lab Inc | 反応アレイ |
US7828949B2 (en) * | 2005-12-08 | 2010-11-09 | Electronics And Telecommunications Research Institute | Biomolecule detection device, mobile phone for biomolecule detection, and biomolecule detection method |
JP4711190B2 (ja) * | 2006-07-28 | 2011-06-29 | 国立大学法人 東京大学 | グリコシル化異常症の検査方法 |
JP5109001B2 (ja) * | 2007-02-27 | 2012-12-26 | 株式会社J−オイルミルズ | α1,6フコース糖鎖の検出および分別方法 |
CN101308141B (zh) * | 2007-05-16 | 2012-08-29 | 陕西北美基因股份有限公司 | 一种分析糖蛋白的方法 |
CN101266251A (zh) * | 2008-04-25 | 2008-09-17 | 南通大学附属医院 | 玻璃微柱亲和层析测定肝癌特异性afp的方法 |
EP2204651A1 (en) * | 2009-01-06 | 2010-07-07 | Shiming Lin | Electrosensing antibody-probe detection and measurement sensor and method |
JP2010236997A (ja) * | 2009-03-31 | 2010-10-21 | Panasonic Corp | マイクロアレイ及びこれを用いた生体情報測定方法 |
CN102043046B (zh) * | 2009-10-13 | 2014-05-07 | 张志丽 | 一种检测糖链异常IgA肾病的蛋白芯片 |
WO2011089988A1 (ja) * | 2010-01-21 | 2011-07-28 | 株式会社J-オイルミルズ | 膵臓癌の検出方法 |
CN102081100B (zh) * | 2010-07-20 | 2015-06-24 | 李伯安 | 一种肝癌多标志物微阵列试剂盒、其制备方法及其应用 |
JP5648613B2 (ja) * | 2011-09-12 | 2015-01-07 | コニカミノルタ株式会社 | 表面プラズモン励起増強蛍光分光法用センサチップおよびそれを用いた測定方法 |
JP5835335B2 (ja) * | 2011-09-15 | 2015-12-24 | コニカミノルタ株式会社 | 表面プラズモン共鳴及び表面プラズモン励起増強蛍光分光法を用いた特定のアナライトの定量測定方法 |
JP5726038B2 (ja) * | 2011-09-30 | 2015-05-27 | コニカミノルタ株式会社 | 表面プラズモン励起増強蛍光分光法を用いた前立腺特異抗原の定量方法 |
JP5991032B2 (ja) * | 2012-06-07 | 2016-09-14 | コニカミノルタ株式会社 | レクチンを用いたアナライトの検出方法 |
CN103822878A (zh) * | 2012-11-16 | 2014-05-28 | 上海市肿瘤研究所 | 凝集素功能化的纳米金及其制备方法和应用 |
CN103336126B (zh) * | 2013-05-08 | 2015-12-02 | 西北大学 | 一种针对唾液样本的凝集素测试芯片及其处理方法 |
CN104678103A (zh) * | 2014-08-05 | 2015-06-03 | 首都医科大学附属北京佑安医院 | 检测血清糖蛋白岩藻糖指数的化学发光蛋白芯片、试剂盒及检测方法 |
-
2014
- 2014-12-12 CN CN201410772665.4A patent/CN104678103A/zh active Pending
-
2015
- 2015-02-04 CN CN201510058591.2A patent/CN104849468B/zh not_active Expired - Fee Related
- 2015-02-13 US US14/622,259 patent/US20160223556A1/en not_active Abandoned
- 2015-02-16 CA CA2882151A patent/CA2882151C/en not_active Expired - Fee Related
- 2015-02-23 JP JP2015033234A patent/JP6082767B2/ja not_active Expired - Fee Related
- 2015-07-17 WO PCT/CN2015/084356 patent/WO2016019797A1/zh active Application Filing
- 2015-07-17 KR KR1020187009587A patent/KR101914673B1/ko active IP Right Grant
- 2015-07-17 KR KR1020177006041A patent/KR20170040318A/ko active Application Filing
- 2015-07-17 GB GB1703351.5A patent/GB2548978B/en not_active Expired - Fee Related
- 2015-07-17 SE SE1750227A patent/SE1750227A1/sv not_active Application Discontinuation
-
2016
- 2016-08-10 AU AU2016101432A patent/AU2016101432A4/en not_active Ceased
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN2613759Y (zh) * | 2003-03-31 | 2004-04-28 | 穆海东 | 一种蛋白质芯片 |
CN101603966A (zh) * | 2008-06-12 | 2009-12-16 | 上海裕隆生物科技有限公司 | 一种男性多肿瘤标志物检测蛋白芯片及其试剂盒 |
CN102695716A (zh) * | 2009-07-14 | 2012-09-26 | 独立行政法人产业技术综合研究所 | 肝病病情指标糖链标记物 |
CN103823058A (zh) * | 2014-02-27 | 2014-05-28 | 首都医科大学附属北京佑安医院 | 血清中抗原类蛋白的化学发光蛋白芯片方法和试剂盒 |
Non-Patent Citations (1)
Title |
---|
血清甲胎蛋白水平与甲胎蛋白异质体百分比在诊断肝细胞癌中的临床价值;孙红 等;《蚌埠医学院学报》;20140731;第39卷(第7期);摘要、第960页左栏第1段,第961页右栏第2-6段 * |
Also Published As
Publication number | Publication date |
---|---|
WO2016019797A1 (zh) | 2016-02-11 |
GB2548978A (en) | 2017-10-04 |
JP2016038375A (ja) | 2016-03-22 |
US20160223556A1 (en) | 2016-08-04 |
GB2548978B (en) | 2019-08-21 |
CN104678103A (zh) | 2015-06-03 |
CA2882151C (en) | 2017-10-03 |
CN104849468A (zh) | 2015-08-19 |
GB201703351D0 (en) | 2017-04-19 |
CA2882151A1 (en) | 2016-02-02 |
SE1750227A1 (en) | 2017-03-02 |
AU2016101432A4 (en) | 2016-09-08 |
GB2548978A8 (en) | 2018-10-10 |
JP6082767B2 (ja) | 2017-02-15 |
KR101914673B1 (ko) | 2018-11-02 |
KR20180039184A (ko) | 2018-04-17 |
KR20170040318A (ko) | 2017-04-12 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104849468B (zh) | 检测血清糖蛋白岩藻糖指数的化学发光蛋白芯片、试剂盒及检测方法 | |
CN109374903A (zh) | 一种超灵敏il-6荧光免疫层析测定试剂盒及测定方法 | |
CN101603966A (zh) | 一种男性多肿瘤标志物检测蛋白芯片及其试剂盒 | |
CN102159591A (zh) | 单克隆抗体和使用所述单克隆抗体的免疫测定法 | |
CN108291912B (zh) | 减少免疫测定法中的干扰的方法 | |
CN101363860A (zh) | 梅毒螺旋体抗体化学发光免疫分析测定试剂盒及其制备方法 | |
CN108333344A (zh) | 高灵敏度的化学发光免疫分析试剂盒及其制备方法和应用 | |
CN107727864A (zh) | 一种检测血清中异常脱羧凝血酶原的蛋白芯片、试剂盒及其制备方法 | |
Khosravi et al. | Immunofluorometry of choriogonadotropin by time-resolved fluorescence spectroscopy, with a new europium chelate as label. | |
TW201643429A (zh) | 前列腺抗原標準品及其用途 | |
Ekins | Ambient analyte assay | |
CN103823058B (zh) | 血清中抗原类蛋白的化学发光蛋白芯片方法和试剂盒 | |
CN109001471A (zh) | 游离人绒毛膜促性腺激素β亚单位化学发光检测试剂盒及其制备方法和使用方法 | |
CN101377509A (zh) | Iii型前胶原n端肽化学发光免疫分析定量测定试剂盒及其制备方法 | |
CN108982482A (zh) | 抑制素b化学发光免疫检测试剂盒及其制备方法 | |
CN108872595A (zh) | 一种癌胚抗原检测试剂盒及其制备方法 | |
CN1924581A (zh) | 前列腺特异性抗原化学发光定量检测试剂盒 | |
CN110687293A (zh) | 一种用于检测胃癌抗原MG7-Ag的试剂盒及其应用 | |
CN107748261A (zh) | 一种检测血清中异常脱羧凝血酶原的蛋白芯片、试剂盒及其制备方法 | |
CN103645320A (zh) | 一种呋喃它酮代谢物化学发光酶联免疫快速检测试剂盒 | |
Yan et al. | Technical and clinical comparison of two fully automated methods for the immunoassay of CA 125 in serum | |
CN109313193A (zh) | 消化道癌的判定方法 | |
CN107436350B (zh) | 一种利用酶标板和单克隆抗体进行混合物中各成分的分离方法 | |
CN106248959B (zh) | 一种检测血清岩藻糖蛋白的免疫渗滤方法及免疫渗滤装置 | |
CN201247246Y (zh) | 一种男性多肿瘤标志物检测蛋白芯片及其试剂盒 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
EXSB | Decision made by sipo to initiate substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Li Ning Inventor after: Zhang Aiying Inventor after: Wang Shengqi Inventor after: Ke Yang Inventor before: Li Ning Inventor before: Zhang Aiying Inventor before: Wang Shengqi Inventor before: Ke Yang Inventor before: Zhang Yonghong |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180213 Termination date: 20210204 |