A kind of method utilizing waste material of edible mushroom to cultivate Taxus x media bag seedling
Technical field
The present invention relates to a kind of method utilizing waste material of edible mushroom to cultivate Taxus x media bag seedling, belong to forestry cultivation field.
Background technology
Taxus x media (Taxusmedia) is the crossbreed of taxus chinensis in northeast and european yew, evergreen shrubs, is important Ornamental trees and precious medicinal plant.Taxol contained by it has unique antitumor action.This seeds content of taxol reaches 0.03% ~ 0.06%, and be 4 ~ 10 times of domestic variety, and plant each several part is all containing taxol, extraction and application rate is high.Add the advantages such as vitality, resistant to diseases and insects are strong, Taxus x media market prospects are considerable.
Because Taxus x media belongs to Hybrid, seeding and seedling raising easily morphs, and for keeping kind pure, is generally only limited to the vegetative manner such as cuttage.The conventional breeding of current Taxus x media cuttage is main is husky with yellow soil, riverbed and perlite, sand, yellow soil and organic cooperation are cultivated on seedbed as matrix in method, takes root for being transplanted in nutrition cup after surviving for cultivating again.Although yellow soil inserts skewer nursery also have fast, survival rate high of taking root, yellow soil permeability is poor, seedling root poor growth, and after watering, soil easily hardens, and be unfavorable for that nursery stock is long, and yellow soil is heavier, is inconvenient to afforestation of transporting and go up a hill.Although the husky permeability in riverbed is good, but the husky nutrition in riverbed is poor, it is unfavorable to grow after taking root to plant, therefore, filter out a kind of simple to operate, survival rate is high, Light media cuttage and seedling culture method light again, to quickening Taxus x media clonal reproduction, for vast farmers provides the seedling of high-quality, meeting Taxus x media, to produce growth requirement significant.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method utilizing waste material of edible mushroom to cultivate Taxus x media bag seedling, the present invention adopts the Light media cottage breeding Taxus x media bag seedling based on waste material of edible mushroom, cultivation period is short, plant percent is high, quality is good, simple to operate, cost is low, be easy to factorial praluction, is one asexual reproduction method fast.
The technical scheme that the present invention solves the problems of the technologies described above is as follows: a kind of method utilizing waste material of edible mushroom to cultivate Taxus x media bag seedling, comprising:
1) preparation of Light media: with the percentages of raw material cumulative volume, get the waste material of edible mushroom of 30% ~ 40%, 5% ~ 15% coconut palm chaff, the peat of 5% ~ 15%, bark, the perlite of 5% ~ 15%, the husk of 5% ~ 15% of 20% ~ 35%, mix, be produced into the nutritious bag of diameter 40mm, high 80mm with Light media net-bag seedling container forming machine;
2) process of cuttings: gather branch from Taxus x media elite stand, as cuttings, uses carbendazim sterilization;
3) rooting treatment: cuttings base portion 1 ~ 3cm indolebutyric acid solution is soaked 1 ~ 2h;
4) cottage method: the cuttings that will be moistened with indolebutyric acid solution inserts 1) in the nutritious bag prepared, the degree of depth is that 1/3 of 3cm or cutting length is advisable, and is plugged rear water and irrigates;
5) final-period management:
1. heat and moisture preserving: by 4) nutritious bag after process is placed on seedbed, and it is highly the shed of 50cm that seedbed is set up, with covered rearing with plastic film, with earth, surrounding is compressed, by whole for seedbed good seal, to keep in shed 20 ~ 30 DEG C, relative moisture more than 90%, Light media moisture content 50% ~ 60%;
2. Shading treatment: the frame building high 200cm on seedbed, coverage shading net on frame, shading rate is 70% ~ 80%;
3. the extermination of disease and insect pest: opened plastic film once every 7 ~ 10 days, sprays insecticide;
4. apply fertilizer: from generation callus to take root, sprayed foliage fertilizer every 7 ~ 10 days, be sprayed onto till blade face is covered with the globule; When cuttings when 80% has been taken root or cuttings obviously sends sprouting, every 10 ~ 15 days sealing fertilizers, concentration progressively increased from 75 ~ 150mg/L, and consumption is with till solution saturates Light media.
On the basis of technique scheme, the present invention can also do following improvement.
Further, 1) in, described waste material of edible mushroom is culture medium waste remaining after gathering in the crops product in edible fungus culturing process, mainly containing cotton seed hulls, wood sawdust, bagasse, wheat bran etc.After measured, containing crude protein 7 ~ 13%, crude fat 0.2 ~ 4%, raw fiber 20 ~ 30%, ash content 10 ~ 35%, calcium 0.3 ~ 1.8%, phosphorus 0.07 ~ 0.3% and potassium, zinc, magnesium, copper, iron, manganese, the trace element such as to receive, nutrient composition content enriches.
Further, 2) in, choose raw then lignification or the semi-lignified branch of the Taxus x media elite stand of vigorous, the anosis worm of growing way, branch is cut into long 10 ~ 15cm, the thick 0.3 ~ 0.5cm in footpath, upper end is truncated, undercut angle at 45 °, removes the blade of branch bottom 2/3, as cuttings, then soak 5 ~ 10min with 0.07% carbendazim solution, clean with clear water after immersion.
Further, 3) in, the preparation of described indolebutyric acid solution: by 1g purity be 98% indolebutyric acid crystal first dissolve with the ethanol of 5 ~ 10ml95%, then be diluted with distilled water into the solution that concentration is 200mg/L.
Further, 4) in, being first soaked in water by Light media before cuttage, is 0.3% potassium permanganate spray sterilization process by concentration again after all permeable.
Further, 5) in, described foliage fertilizer is concentration is the urea of 0.2% or the potassium dihydrogen phosphate water liquid fertilizer of 0.2%, and both alternately spray.
Further, 5) in, containing of described composite fertilizer: nitrogen: phosphorus: potassium=15:15:15.
The invention discloses a kind of method utilizing waste material of edible mushroom Light media cottage breeding Taxus x media bag seedling, have the following advantages relative to prior art:
1, the Light media material source that adopts of the present invention is easy, cost is low, easy processing, mechanization production degree of one-tenth are high, be easy to factorial praluction;
2, the Light media water imbibition, the permeability that adopt of the present invention is better, and the content of organic matter is high, is beneficial to plant root growth, significantly improves Taxus x media cuttage survival rate;
3, the Light media of the present invention's employing is compared with traditional yellow soil, the husky cuttage in riverbed, decrease migration process again, and the nursery stock that Light media is cultivated is lightweight, is convenient to transport, therefore, reduces production cost and cost of forestation;
4, cuttage and seedling culture method of the present invention maintains the merit of Taxus x media elite stand, and reproduction coefficient is high, cost is low, simple to operate, is easy to production application.
Embodiment
Be described principle of the present invention and feature below, example, only for explaining the present invention, is not intended to limit scope of the present invention.
Utilize waste material of edible mushroom to cultivate the step-by-step procedures of the method for Taxus x media bag seedling below for the present invention is a kind of:
1) preparation of Light media: with the percentages of raw material cumulative volume, get the waste material of edible mushroom of 30% ~ 40%, 5% ~ 15% coconut palm chaff, the peat of 5% ~ 15%, bark, the perlite of 5% ~ 15%, the husk of 5% ~ 15% of 20% ~ 35%.Waste material of edible mushroom mixes rear plastic film covering with peat, pile fermentation 40 ~ 50d; Husk carries out carbonization treatment by retort; Bark by cracker be ground into after below 0.5cm fragment with coconut palm chaff pile fermentation 50 ~ 60d.After above raw material fully becomes thoroughly decomposed, Homogeneous phase mixing together, is produced into the nutritious bag of diameter 40mm, high 80mm with Light media net-bag seedling container forming machine.First Light media is soaked in water before cuttage, after all permeable, uses 0.3% potassium permanganate spray sterilization process again.
2) process of cuttings: the clip raw thick 0.3 ~ 0.5cm in footpath then from the Taxus x media elite stand that health is disease-free, long 10 ~ 15cm lignification or semi-lignified branch, upper end is truncated, undercut angle at 45 °, remove the blade of branch bottom 2/3, as cuttings, then soak 5 ~ 10min with 0.07% carbendazim solution, clean with clear water after immersion.
3) rooting treatment: be that the indolebutyric acid of 98% is first with after the ethanol dissolving of 5 ~ 10ml95% by 1g purity, be diluted with distilled water into the solution that concentration is 200mg/L again, cuttings base portion 1 ~ 3cm indolebutyric acid solution is soaked 2h, avoids in immersion process allowing blade and tender shoots be stained with soak.
4) cottage method: the cuttings being moistened with indolebutyric acid solution inserted in time in nutritious bag, fall to insert or oblique cutting, compacting Light media after cuttings cuttage, makes Light media and cuttings close contact, and the degree of depth is that 1/3 of 3cm or cutting length is advisable, and is plugged rear water and irrigates.
5) final-period management:
1. heat and moisture preserving: by 4) process after nutritious bag be placed on seedbed, it is highly the shed of 50cm that seedbed is set up, with covered rearing with plastic film, with earth, surrounding is compressed, by whole for seedbed good seal, when Light media is more dry, increase humidity with spraying, number of times is decided as circumstances require, spray have the thin globule with cuttings blade face but do not drip, hand touches Light media that wet ravine is felt, hand grabs that Light media is not agglomerating is advisable.Produce before callus, to keep in shed 20 ~ 30 DEG C, relative moisture more than 90%, Light media moisture content 50% ~ 60%, later relative moisture can keep 80%.
2. Shading treatment: the frame building high 200cm on seedbed, coverage shading net on frame, shading rate is 70% ~ 80%;
3. the extermination of disease and insect pest: opened plastic film once every 7 ~ 10 days after cuttage, and be used alternatingly spraying control with carbendazim, the topsin of 0.05% ~ 0.1%, the zineb solution of 0.05% ~ 0.1% of 0.05% ~ 0.1%;
4. apply fertilizer: from generation callus to take root, sprayed foliage fertilizer every 7 ~ 10 days, alternately spray 0.2% urea and 0.2% potassium dihydrogen phosphate water liquid fertilizer, be sprayed onto till blade face is covered with the globule; When cuttings when 80% has been taken root or cuttings obviously sends sprouting, every 10 ~ 15 days sealing fertilizers (nitrogen: phosphorus: potassium=15:15:15), concentration progressively increased from 75 ~ 150mg/L, and consumption is with till solution saturates Light media.
Embodiment 1
1) preparation of Light media: with the percentages of raw material cumulative volume, get waste material of edible mushroom 30%, coconut palm chaff 15%, peat 5%, husk 5%, bark 30%, perlite 15%, waste material of edible mushroom mixes rear plastic film covering with peat, pile fermentation 40 ~ 50d; Husk carries out carbonization treatment by retort; Bark by cracker be ground into after below 0.5cm fragment with coconut palm chaff pile fermentation 50 ~ 60d.After above raw material fully becomes thoroughly decomposed, Homogeneous phase mixing together, is produced into the nutritious bag of diameter 40mm, high 80mm with Light media net-bag seedling container forming machine.First Light media is soaked in water before cuttage, after all permeable, uses 0.3% potassium permanganate spray sterilization process again.
2) process of cuttings: in March, 2013 carries out cuttage, thick 0.3 ~ the 0.5cm in the annual footpath of clip from health disease-free 9 years raw Taxus x media elite stands, long 10 ~ 15cm lignification or semi-lignified branch, upper end is truncated, undercut angle at 45 °, remove the blade of branch bottom 2/3, as cuttings, then soak 5 ~ 10min with 0.07% carbendazim solution, clean with clear water after immersion.
3) rooting treatment: be that the indolebutyric acid of 98% is first with after the ethanol dissolving of 5 ~ 10ml95% by 1g purity, be diluted with distilled water into the solution that concentration is 200mg/L again, cuttings base portion 2 ~ 3cm indolebutyric acid solution is soaked 1h, avoids in immersion process allowing blade and tender shoots be stained with soak.
4) cottage method: the cuttings being moistened with indolebutyric acid solution inserted in time in nutritious bag, fall to insert or oblique cutting, compacting Light media after cuttings cuttage, makes Light media and cuttings close contact, and the degree of depth is that 1/3 of 3cm or cutting length is advisable, and is plugged rear water and irrigates.
5) final-period management:
1. heat and moisture preserving: by 4) process after nutritious bag be placed on seedbed, it is highly the shed of 50cm that seedbed is set up, with covered rearing with plastic film, with earth, surrounding is compressed, by whole for seedbed good seal, open plastic film early morning when temperature is large and carry out ventilation, when Light media is more dry, increase humidity with spraying, number of times is decided as circumstances require, spray have the thin globule with cuttings blade face but do not drip, hand touches Light media that wet ravine is felt, hand grabs that Light media is not agglomerating is advisable.Produce before callus, to keep in shed 20 ~ 30 DEG C, relative moisture more than 90%, Light media moisture content 50% ~ 60%, later relative moisture can keep 80%.
2. Shading treatment: the frame building high 200cm on seedbed, coverage shading net on frame, shading rate is 70% ~ 80%;
3. the extermination of disease and insect pest: opened plastic film once every 7 ~ 10 days after cuttage, and be used alternatingly spraying control with carbendazim, the topsin of 0.05% ~ 0.1%, the zineb of 0.05% ~ 0.1% of 0.05% ~ 0.1%;
4. apply fertilizer: from generation callus to take root, sprayed foliage fertilizer every 7 ~ 10 days, alternately spray 0.2% urea and 0.2% potassium dihydrogen phosphate water liquid fertilizer, be sprayed onto till blade face is covered with the globule; After taking root May, every 10 ~ 15 days sealing fertilizers (nitrogen: phosphorus: potassium=15:15:15), concentration progressively increased from 75 ~ 150mg/L, and consumption is with till solution saturates Light media.
After cuttage, sampling observation in 20 days has obvious callus to occur, cuttage 65 days sample survey results are cuttage seeding rooting rate is 78.2%, and young sprout rate is 66.7%.Measure cuttings survival rate 82.4% December, taking out tip rate is 87.3%.
Embodiment 2
1) preparation of Light media: with the percentages of raw material cumulative volume, get the waste material of edible mushroom of 35%, 10% coconut palm chaff, the peat of 10%, bark, the perlite of 10%, the husk of 10% of 25%.Waste material of edible mushroom mixes rear plastic film covering with peat, pile fermentation 40 ~ 50d; Husk carries out carbonization treatment by retort; Bark by cracker be ground into after below 0.5cm fragment with coconut palm chaff pile fermentation 50 ~ 60d.After above raw material fully becomes thoroughly decomposed, Homogeneous phase mixing together, is produced into the nutritious bag of diameter 40mm, high 80mm with Light media net-bag seedling container forming machine.First Light media is soaked in water before cuttage, after all permeable, uses 0.3% potassium permanganate spray sterilization process again.
2) process of cuttings: in June, 2013 carries out cuttage, the clip raw thick 0.3 ~ 0.5cm in footpath then from health disease-free 9 years raw Taxus x media elite stands, long 10 ~ 15cm lignification or semi-lignified branch, upper end is truncated, undercut angle at 45 °, remove the blade of branch bottom 2/3, as cuttings, then soak 5 ~ 10min with 0.07% carbendazim solution, clean with clear water after immersion.
3) rooting treatment: be that the indolebutyric acid of 98% is first with after the ethanol dissolving of 5 ~ 10ml95% by 1g purity, be diluted with distilled water into the solution that concentration is 200mg/L again, cuttings base portion 2 ~ 3cm indolebutyric acid solution is soaked 1.5h, avoids in immersion process allowing blade and tender shoots be stained with soak.
4) cottage method: the cuttings being moistened with indolebutyric acid solution inserted in time in nutritious bag, fall to insert or oblique cutting, compacting Light media after cuttings cuttage, makes Light media and cuttings close contact, and the degree of depth is that 1/3 of 3cm or cutting length is advisable, and is plugged rear water and irrigates.
5) final-period management:
1. heat and moisture preserving: by 4) process after nutritious bag be placed on seedbed, it is highly the shed of 50cm that seedbed is set up, with covered rearing with plastic film, with earth, surrounding is compressed, by whole for seedbed good seal, when Light media is more dry, increase humidity with spraying, number of times is decided as circumstances require, spray have the thin globule with cuttings blade face but do not drip, hand touches Light media that wet ravine is felt, hand grabs that Light media is not agglomerating is advisable.Produce before callus, to keep in shed 20 ~ 30 DEG C, relative moisture more than 90%, Light media moisture content 50% ~ 60%, later relative moisture can keep 80%.
2. Shading treatment: the frame building high 200cm on seedbed, coverage shading net on frame, shading rate is 70% ~ 80%;
3. the extermination of disease and insect pest: opened plastic film once every 7 ~ 10 days after cuttage, and be used alternatingly spraying control with carbendazim, the topsin of 0.05% ~ 0.1%, the zineb of 0.05% ~ 0.1% of 0.05% ~ 0.1%;
4. apply fertilizer: from generation callus to take root, sprayed foliage fertilizer every 7 ~ 10 days, alternately spray 0.2% urea and 0.2% potassium dihydrogen phosphate water liquid fertilizer, be sprayed onto till blade face is covered with the globule; After taking root mid-July, every 10 ~ 15 days sealing fertilizers (nitrogen: phosphorus: potassium=15:15:15), concentration progressively increased from 75 ~ 150mg/L, and consumption is with till solution saturates Light media.
After cuttage, sampling observation in 15 days has obvious callus to occur, cuttage 45 days sample survey results are cuttage seeding rooting rate is 83.5%, and young sprout rate is 77.3%.Measure cuttings survival rate 92.6% December, taking out tip rate is 91.6%.
Embodiment 3
1) preparation of Light media: with the percentages of raw material cumulative volume, get the waste material of edible mushroom of 40%, 5% coconut palm chaff, the peat of 15%, bark, the perlite of 5%, the husk of 15% of 20%.Waste material of edible mushroom mixes rear plastic film covering with peat, pile fermentation 40 ~ 50d; Husk carries out carbonization treatment by retort; Bark by cracker be ground into after below 0.5cm fragment with coconut palm chaff pile fermentation 50 ~ 60d.After above raw material fully becomes thoroughly decomposed, Homogeneous phase mixing together, is produced into the nutritious bag of diameter 40mm, high 80mm with Light media net-bag seedling container forming machine.First Light media is soaked in water before cuttage, after all permeable, uses 0.3% potassium permanganate spray sterilization process again.
2) process of cuttings: in September, 2013 carries out cuttage, the clip raw thick 0.3 ~ 0.5cm in footpath then from health disease-free 9 years raw Taxus x media elite stands, long 10 ~ 15cm lignification or semi-lignified branch, upper end is truncated, undercut angle at 45 °, remove the blade of branch bottom 2/3, as cuttings, then soak 5 ~ 10min with 0.07% carbendazim solution, clean with clear water after immersion.
3) rooting treatment: be that the indolebutyric acid of 98% is first with after the ethanol dissolving of 5 ~ 10ml95% by 1g purity, be diluted with distilled water into the solution that concentration is 200mg/L again, cuttings base portion 2 ~ 3cm indolebutyric acid solution is soaked 2h, avoids in immersion process allowing blade and tender shoots be stained with soak.
4) cottage method: the cuttings being moistened with indolebutyric acid solution inserted in time in nutritious bag, fall to insert or oblique cutting, compacting Light media after cuttings cuttage, makes Light media and cuttings close contact, and the degree of depth is that 1/3 of 3cm or cutting length is advisable, and is plugged rear water and irrigates.
5) final-period management:
1. heat and moisture preserving: by 4) process after nutritious bag be placed on seedbed, it is highly the shed of 50cm that seedbed is set up, with covered rearing with plastic film, with earth, surrounding is compressed, by whole for seedbed good seal, when Light media is more dry, increase humidity with spraying, number of times is decided as circumstances require, spray have the thin globule with cuttings blade face but do not drip, hand touches Light media that wet ravine is felt, hand grabs that Light media is not agglomerating is advisable.Produce before callus, to keep in shed 20 ~ 30 DEG C, relative moisture more than 90%, Light media moisture content 50% ~ 60%, later relative moisture can keep 80%.
2. Shading treatment: the frame building high 200cm on seedbed, coverage shading net on frame, shading rate is 70% ~ 80%;
3. the extermination of disease and insect pest: opened plastic film once every 7 ~ 10 days after cuttage, and be used alternatingly spraying control with carbendazim, the topsin of 0.05% ~ 0.1%, the zineb of 0.05% ~ 0.1% of 0.05% ~ 0.1%;
4. apply fertilizer: from generation callus to take root, sprayed foliage fertilizer every 7 ~ 10 days, alternately spray 0.2% urea and 0.2% potassium dihydrogen phosphate water liquid fertilizer, be sprayed onto till blade face is covered with the globule; After taking root November, every 10 ~ 15 days sealing fertilizers (nitrogen: phosphorus: potassium=15:15:15), concentration progressively increased from 75 ~ 150mg/L, and consumption is with till solution saturates Light media.
After cuttage, sampling observation in 20 days has obvious callus to occur, cuttage 50 days sample survey results are cuttage seeding rooting rate is 75.4%, and young sprout rate is 68.5%.Measure cuttings survival rate 79.8% December, taking out tip rate is 87.2%
In above 3 kinds of different proportion Light media, waste material of edible mushroom 35%, coconut palm chaff 10%, peat 10%, husk 10%, bark 25%, perlite 10% substrate composition cuttings survival rate, to take out tip rate be best.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.