CN1220423C - Quick taxus mandia propagating method - Google Patents
Quick taxus mandia propagating method Download PDFInfo
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- CN1220423C CN1220423C CN 03117884 CN03117884A CN1220423C CN 1220423 C CN1220423 C CN 1220423C CN 03117884 CN03117884 CN 03117884 CN 03117884 A CN03117884 A CN 03117884A CN 1220423 C CN1220423 C CN 1220423C
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Abstract
The present invention relates to a quick Taxus madia breeding method which uses the 'totipotency' and the 'holographic property' of Taxus madia. The present invention takes plant tissue culture as a principle which combines with the techniques of normal cutting seedling breeding and cutting seedling breeding in full light and fog, and takes a part of the organ of the Taxus madia (the miniature materials of the Taxus madia) which is processed with 'G. P clone fluid infusion' prepared by being researched and developed to be directly planted in the sorghum beds (or sandy soil) of fields, and the organ of the Taxus madia is combined with the 'G. P clone fluid infusion ' and matching management technology to be managed, and can make roots grown about 25 days. The present invention has the characteristics of small, high culturing speed, high reproduction rate, low cost, easy popularization, no variation of breeding seedlings, etc. The present invention overcomes the technical problems of the former technology for breeding the Taxus madia, such as large material, long breeding time, low survival rate, high cost, difficult popularization, etc.
Description
Technical field
The present invention relates to a kind of plant propagation renovation process, especially Taxus x media microbody method for quickly breeding.
Background technology
Taxus x media, taxaceae, Taxus is the crossbreed of taxus chinensis in northeast and european yew, is the world rare and endangered species that carry over behind the quaternary glacier, China first class of protection plant.Originate in the acid sandy mountain region of the Canada and the U.S., height above sea level 100-2000 rice.China had the only tens thousand of strains of female parent now from Canada's introduction in 1996 in China, and resource is very rare.This tree body contains very expensive anticancer raw material---taxol, is one of the most medicable cancer therapy drug of the present whole world.1992, U.S. FDA was ratified taxol is used for the treatment of the metastatic oophoroma first, after again approval be used for the treatment of the metastatic breast cancer in late period.The taxol synthesis pharmaceutical is used in country's listings such as the U.S., Britain, Canada, Sweden, Austria at present.China Ministry of Public Health also in the preparation of nineteen ninety-five approval taxol and bulk drug as II kind new medicine (Western medicine), be used for the treatment of oophoroma, breast cancer, lung cancer and nasopharyngeal carcinoma etc.
Taxus x media fast growth, lateral root prosperity; strong to adaptive capacity to environment; can be widely used in building soil and water conservation forest, water conservation forest, be one of economical seeds of the state key ecological construction engineering first-selections such as wildwood protected project project of conceding the land to forestry implemented.
The Taxus x media resource is very rare; in China growth history in 6 years only; up to the present also there is not the complete feasible efficient raising technology of a cover; in order to protect China's wild taxus resource in imminent danger; enlarge the population of high-quality Chinese yew; realize artificial high-quality Chinese yew industrialization plantation, become the sector brainstrust and want the difficult problem that solves always.
Summary of the invention
The purpose of this invention is to provide a kind of draw materials little, the time of breeding is short, survival rate is high, cost is low, the Taxus x media method for quickly breeding of easy popularization.
This propagation method, be " totipotency " and " holographic " of utilizing Taxus x media, with the Plant Tissue Breeding is principle, and grow seedlings and full optical haze cuttage raise seedling technique in conjunction with normal cutting propagation, a part (the miniature material of Taxus x media) of getting the Taxus x media organ is after " GP clones fluid infusion " of research and development handles, directly be seeded on the husky bed (or sandy soil) in land for growing field crops, the back manages in conjunction with " GP clones fluid infusion " and supporting management technology, can take root through 25 days approximately.
Concrete scheme of the present invention is: a kind of Taxus x media method for quickly breeding, carry out as follows:
(1) the grow seedlings bed and the preparation of seedling growth greenhouse
A: direction inclined to one side 5 ° to the west of the bed of growing seedlings is made bed, and long 8 meters of seedbed is wide 2.7 meters, deep-cuts a trench in the bed, long 8 meters of ditch, and wide 30cm, dark 40cm, trench is divided into two equal little seedbeds with the seedbed, and each is wide 1.2 meters.
B: above the seedbed, take little shed (is material with the bamboo chip), require canopy high 12 meters, long 8 meters of canopy; And cover white plastic film (thickness is not limit), and long 11 meters of film, two canopy mouths difference sagging 1.5 is in order to cover the canopy mouth.
C: river sand (thickness all can) or sandy soil are tiled on the little seedbed, and thickness is 5cm, requires sand bed soft behind the shop, and with 800 times of liquid of potassium permanganate sand bed is sterilized, and canopy mouth plastic foil is left behind covers the canopy mouth, and it is standby to raise plastic foil after 24 hours.
(2) No. 1, a cover fluid infusion " GP clones fluid infusion " of the suitable Taxus x media growth of preparation:
IBA (indolebutyric acid) 1540～1660mg
NAA (methyl) 242～265mg
6-BA (6-benzylaminopurine) 0.4～0.56mg
Distilled water adds to 1000ml
Main points: make mixed liquor according to concentration, with a spot of 95% alcohol dissolving IBA and NAA, after add an amount of water again.6-BA uses 1molL earlier
-1HCl dissolving, it is fixed molten to add water again.After preparation finishes, measure its pH value with acidometer, the pH value of this solution is 6.0, if meta-acid 1molL
--1NaOH regulates, if alkali is regulated with 1molL-1HCl partially.
" GP clones fluid infusion " No. 2:
KNO
3(potassium nitrate) 1580～1620mg
CaCl
22H
2O (calcium chloride) 395～410mg
Distilled water adds to 1000ml
" GP clones fluid infusion " No. 3:
NH
4NO
3(ammonium nitrate) 1440～1460mg
MgSO
47H
2O (magnesium sulfate) 325～335mg
KH
2PO
4(potassium dihydrogen phosphate) 96～110mg
Distilled water adds to 1000ml
" GP clones fluid infusion " No. 4:
MnSO
44H
2O (manganese sulphate) 18.5～22.5mg
ZnSO
47H
2O (zinc sulphate) 10～12.5mg
H
3BO
3(boric acid) 9.6～11mg
Distilled water adds to 1000ml
" GP clones fluid infusion " No. 5:
Na
2MoO
42H
2(sodium molybdate) 0.2～0.3mg
CuSO
45H
2O (copper sulphate) 0.025～0.035mg
CoCl
26H
2O (cobalt chloride) 0.025～0.035mg
KI (potassium iodide) 0.85～1.24mg
Distilled water adds to 1000ml
" GP clones fluid infusion " No. 6:
FeSO
47H
2O (iron sulfate) 46～53mg
Na
2-EDTA (disodium ethylene diamine tetraacetate); 38.5～45mg
Distilled water adds to 1000ml
" GP clones fluid infusion " No. 7:
Vit.B6 (pyridoxine hydrochloride) 0.78～0.85mg
Gly (glycine) 2.25～2.65mg
Inositol 75.5～80mg
Coconut palm breast 120～128mg
Distilled water adds to 1000ml
" GP clones fluid infusion " No. 8:
C
2H
22O
11(sucrose) 15800～16150mg
Distilled water adds to 1000ml
" GP clones fluid infusion " No. 9:
Hydrogen peroxide 85000～120000mg
Distilled water adds to 1000ml
(3) female parent is bred the preparation of material
The branch (age of branch is not limit, but should not be thick excessively) of Taxus x media is collected fringe bar need not sterilize (this technology inoculation operating process implements to carry disease germs operation, does not need any sterilization) from elite stand with sharp blade or scissors.
The temperature and humidity that keeps the Taxus x media branch is cut into stem section or stem apex long about 0.5cm with branch under the half-light condition, keep blade, and lower cut keeps flat mouthful.
(4) inoculation
Fast the stem section that cuts or the lower cut of stem apex material are dipped in " GP clones fluid infusion " No. 1 liquid middling speed, the speed time of dipping in is no more than 1 second, and it is 0.5～0.15cm that material is dipped the degree of depth.
Material after dipping is seeded on the husky bed in the treated little shed immediately, requires husky bed humidity to pinch not agglomerating being advisable with husky fist, the inoculation degree of depth is that the maternal material of the husky bed of lower cut contact is not advisable.Inoculum density is every square metre of 800-1000 strain.After inoculation finished, with seedbed and little shed spraying one time (this technology adopts the artificial sprayer of rod-pulling type manually to spray, with better adjusting spray intensity), intensity was advisable with the blade face No drip type with " GP clones fluid infusion " No. 9 liquid.
(5) postvaccinal management
Temperature:
Inoculate the seedling management Taxus x media 4-10 month, general temperature of shed was controlled at the 25-28 degree and was advisable daytime, regulate the moisture cooling if temperature is too high by spraying, if temperature is crossed the low method of envelope canopy mouth that then adopts and is heated, front cover part canopy mouth in evening (promptly staying osculum), temperature of shed generally is controlled at the 15-20 degree, but will note ventilating.
Taxus x media is inoculated the seedling management November to March next year, and daytime, general temperature of shed was controlled at 12-16 degree (it is better that the ground hot line is laid in the seedbed), and temperature is crossed the low canopy that can seal and controlled temperature, but will ventilate at any time.General temperature of shed was controlled at the 8-12 degree and was advisable evening.
Humidity (moisture):
Moisture management mainly contains humidity in the canopy, inoculation seedling blade face humidity and seedbed moisture management; 4-10 month is inoculated, inoculation seedling blade face humidity requirement keeps the very thin moisture film of one deck constantly, promptly do promptly and mend, the moisturizing instrument is the rod-pulling type sprayer, method is artificial spraying (if employing auto spraying or computer control spraying then do not reach desirable effect, survival rate reduces), and this is the precondition that guarantees interior humidity of canopy and seedbed humidity, promptly this condition satisfies, and other corresponding conditions also can satisfy; Relatively season, 4-10 month is inoculated, and humidity requirement is bigger than all the other time inoculation desired moisture levels in the canopy, and the seedbed humidity requirement is also corresponding wants big, and management difficulty is also corresponding bigger.
All the other inoculations in season, management is easier to relatively, and it also is the prerequisite that keeps interior humidity of canopy and seedbed humidity that inoculation seedling blade face humidity requirement keeps the very thin moisture film of one deck constantly, just comparatively speaking, it is little that the interior humidity requirement of canopy is inoculated desired moisture level than 4-10 month, and the seedbed humidity requirement is also corresponding little.
Illumination:
Inoculate seedling 4-10 month and will carry out shading treatment daytime, can be with 75% shading net shading, available in case of necessity 2 layers of shading net (partial blade is arranged on the inoculation material, and suitable illumination can be carried out photosynthesis, generation part nutriment) when illumination is strong.All the other time inoculations can be carried out suitable shading or not shading according to actual conditions.Shading net is generally built 2 meters above seedling growth greenhouse.
Ventilate (oxygen and carbonic acid gas):
Ventilation is extremely important in seedling raising process, will open wide two ends canopy mouth when temperature is too high, often ventilates, and temperature is crossed when hanging down and also will be ventilated according to the actual conditions compartment.The commutative canopy wind outer air of ventilating, combustion gas strengthens oxygen and carbonic acid gas, increases the photosynthesis of Taxus x media, and can reduce the generation of disease.
Nutrient (nutrition):
Taxus x media will consume a large amount of nutriment in seedling raising process.In whole seedling raising process, prepared a cover fluid infusion of suitable Taxus x media for this reason.Concrete application program is as follows:
" GP clones fluid infusion " No. 9:
Time of application: 1st, 11,19,27,35 days
" GP clones fluid infusion " No. 2:
Time of application: 4th, 12,20,28 days
" GP clones fluid infusion " No. 3:
Time of application: 5th, 13,21,29 days
" GP clones fluid infusion " No. 6:
Time of application: 6th, 14,22,30 days
" GP clones fluid infusion " No. 7:
Time of application: 7th, 15,23,31 days
" GP clones fluid infusion " No. 8:
Time of application: 8th, 16,24,32 days
" GP clones fluid infusion " No. 4:
Time of application: 9th, 17,25,33 days
" GP clones fluid infusion " No. 5:
Time of application: 10th, 18,26,34 days
Illustrate:
" GP clones fluid infusion " is spray application 2-9 number, is exclusively used in the replenishing of mineral element of Taxus x media.Shared fluid infusion is 35 days in the seedling raising process, wherein 2-3 days need not any fluid infusion,
Fluid infusion every day time of application is last spray time, and spray intensity is advisable with the blade No drip type.
(6) take root, hardening and go out the garden
Through cultivating, in the time of the 25th day, the bed of growing seedlings promptly has the Taxus x media about 60% to take root, and during by the 30th day, rooting rate can reach 99%.30-35 days, alleviate spray intensity gradually, stop to use of fluid infusion in the time of the 36th day, little shed can be raised and carry out hardening, during by the 50th day, seedling has sent tender shoots, can go out the garden and transplant or carry out subculture and breed.
A: the preferred weight proportioning of " GP clones fluid infusion " No. 1 each raw material of liquid is among the present invention:
IBA (indolebutyric acid) 1585～1610mg
NAA (methyl) 248～255mg
6-BA (6-benzylaminopurine) 0.45～0.53mg
Distilled water adds to 1000ml
B: the preferred weight proportioning of " GP clones fluid infusion " No. 2 each raw material of liquid is among the present invention:
KNO
3(potassium nitrate) 1590～1610mg
CaCl
22H
2O (calcium chloride) 398～405mg
Distilled water adds to 1000ml
C: the preferred weight proportioning of " GP clones fluid infusion " No. 3 each raw material of liquid is among the present invention:
NH
4NO
3(ammonium nitrate) 1445～1455mg
MgSO
47H
2O (magnesium sulfate) 326～332mg
KH
2PO
4(potassium dihydrogen phosphate) 98～105mg
Distilled water adds to 1000ml
D: the preferred weight proportioning of " GP clones fluid infusion " No. 4 each raw material of liquid is among the present invention:
MnSO
44H
2O (manganese sulphate) 19.5～21.5mg
ZnSO
47H
2O (zinc sulphate) 10.5～12mg
H
3BO
3(boric acid) 9.8～10.05mg
Distilled water adds to 1000ml
E: the preferred weight proportioning of " GP clones fluid infusion " No. 5 each raw material of liquid is among the present invention:
Na
2MoO
42H
2(sodium molybdate) 0.22～0.29mg
CuSO
45H
2O (copper sulphate) 0.026～0.032mg
CoCl
26H
2O (cobalt chloride) 0.026～0.034mg
KI (potassium iodide) 0.95～1.04mg
Distilled water adds to 1000ml
F: the preferred weight proportioning of " GP clones fluid infusion " No. 6 each raw material of liquid is among the present invention:
FeSO
47H
2O (iron sulfate) 48～52.5mg
Na
2-EDTA (disodium ethylene diamine tetraacetate); 39～44mg
Distilled water adds to 1000ml
G: the preferred weight proportioning of " GP clones fluid infusion " No. 7 each raw material of liquid is among the present invention:
Vit.B6 (pyridoxine hydrochloride) 0.79～0.82mg
Gly (glycine) 2.26～2.62mg
Inositol 76～78.5mg
Coconut palm breast 124～127.5mg
Distilled water adds to 1000ml
H: the preferred weight proportioning of " GP clones fluid infusion " No. 8 each raw material of liquid is among the present invention:
C
2H
22O
11(sucrose) 15940～16050mg
Distilled water adds to 1000ml
I: the preferred weight proportioning of " GP clones fluid infusion " No. 9 each raw material of liquid is among the present invention:
Hydrogen peroxide 88000～110000mg
Distilled water adds to 1000ml
A: the optimum weight proportioning of " GP clones fluid infusion " No. 1 each raw material of liquid is among the present invention:
IBA (indolebutyric acid) 1600mg
NAA (methyl) 250mg
6-BA (6-benzylaminopurine) 0.5mg
Distilled water adds to 1000ml
B: the optimum weight proportioning of " GP clones fluid infusion " No. 2 each raw material of liquid is among the present invention:
KNO
3(potassium nitrate) 1600mg
CaCl
22H
2O (calcium chloride) 400mg
Distilled water adds to 1000ml
C: the optimum weight proportioning of " GP clones fluid infusion " No. 3 each raw material of liquid is among the present invention:
NH
4NO
3(ammonium nitrate) 1450mg
MgSO
47H
2O (magnesium sulfate) 330mg
KH
2PO
4(potassium dihydrogen phosphate) 100mg
Distilled water adds to 1000ml
D: the optimum weight proportioning of " GP clones fluid infusion " No. 4 each raw material of liquid is among the present invention:
MnSO
44H
2O (manganese sulphate) 20mg
ZnSO
47H
20 (zinc sulphate) 11mg
H
3BO
3(boric acid) 10mg
Distilled water adds to 1000ml
E: the optimum weight proportioning of " GP clones fluid infusion " No. 5 each raw material of liquid is among the present invention:
Na
2MoO
42H
2(sodium molybdate) 0.25mg
CuSO
45H
2O (copper sulphate) 0.03mg
CoCl
26H
2O (cobalt chloride) 0.03mg
KI (potassium iodide) 1mg
Distilled water adds to 1000ml
F: the optimum weight proportioning of " GP clones fluid infusion " No. 6 each raw material of liquid is among the present invention:
FeSO
47H
2O (iron sulfate) 50mg
Na
2-EDTA (disodium ethylene diamine tetraacetate); 40mg
Distilled water adds to 1000ml
G: the optimum weight proportioning of " GP clones fluid infusion " No. 7 each raw material of liquid is among the present invention:
Vit.B6 (pyridoxine hydrochloride) 0.8mg
Gly (glycine) 2.6mg
Inositol 78mg
Coconut palm breast 125mg
Distilled water adds to 1000ml
H: the optimum weight proportioning of " GP clones fluid infusion " No. 8 each raw material of liquid is among the present invention:
C
2H
22O
11(sucrose) 16000mg
Distilled water adds to 1000ml
I: the optimum weight proportioning of " GP clones fluid infusion " No. 9 each raw material of liquid is among the present invention:
Hydrogen peroxide 100000mg
Distilled water adds to 1000ml
The present invention is bold in innovation on the basis that plant tissue culture technique, full optical haze cuttage technique, conventional seedbed system technology organically combine, and has formed the brand-new raising technology of a cover, has overcome whole shortcomings of existing seedling growing process.
The present invention does not need any moving from being seeded to out all disposable success in garden, and numerous and diverse operations such as cultivation, culture of rootage are grown in the adventitious bud induction culture, the blastogenesis that have overcome tissue culture.The present invention has added the higher basic element of cell division and growth hormone in " GP clones fluid infusion " No. 1 liquid, can efficiently promote Taxus x media inoculation seedling rooting fast.Simultaneously in incubation, physiology, growth characteristics in conjunction with Taxus x media, the required macroelement of Taxus x media, trace element, organic component, little living element etc. are provided, and in conjunction with conditions such as suitable light, temperature, water, gas, the particularly utilization of light, can make Taxus x media in incubation, carry out photosynthesis, required nutriment in the added body, this has all improved the survival rate of Taxus x media inoculation seedling and the success rate of cultivation greatly.
Adopt method provided by the invention to cultivate, during by the 12nd day, inferior gracefully red bean Chinese larch promptly has a large amount of callus to produce, and small particles appears, have new root to bear in the time of the 17th day, in the time of the 25th day, the bed of growing seedlings promptly has the Taxus x media about 60% to take root, during by the 30th day, rooting rate can reach 99%.30-35 days, alleviate spray intensity gradually, stop to use of fluid infusion in the time of the 36th day, little shed can be raised and carry out hardening, during by the 50th day, seedling has sent tender shoots, can go out the garden and transplant or carry out subculture and breed.
Utilizing the annual available few a large amount of high-quality seedling of maternal material breeding of the present invention, is that existing conventional art institute can not reach.The present invention simultaneously has little, the characteristics such as cultivation speed is fast, reproduction rate is high, cost is low, easy popularization, breeding seedling do not make a variation of drawing materials, and has broken through technical barriers such as drawing materials of the original raising technology of Taxus x media is big, the time of breeding is long, survival rate is low, cost is high, difficult popularization.
Taxus x media can be used seminal propagation, but the time is long, and survival rate is low, existing this method that all do not adopt.Existing Taxus x media raising technology mainly is: tissue culture method, normal cutting propagation method, full optical haze cuttage, but to so far, availability is the end very, and the one, because maternal resource is few, the 2nd, the time of breeding is long, is unfavorable for large-scale production.Existing these three kinds of methods of raising technology and the inventive method are compared, and the present invention can overcome whole shortcomings of existing Taxus x media raising technology, and it is as shown in the table specifically to contrast situation:
Efficient method for quickly breeding of Taxus x media microbody and existing seedling growing process index contrast table
| Technical indicator | Microbody high efficiency quick breeding method | The tissue cultivating method | Full optical haze cuttage | The normal cutting propagation method |
| Breeding condition is bred algebraically/year seedling raise period seedling equipment transfer of technology and is bred cost | Land for growing field crops 5-7 is simple and easy easily lower for the whole year | Laboratory, the annual complicated difference height of test tube 4-6 | The land for growing field crops does not have complicated relatively poor higher | In 2 months 1 generations of land for growing field crops, be simple and easy to low |
| Propagating materials consumes the root system growing way rootage duration rooting rate transplanting success of transplanting seedlings and breeds pollution rate and promote and produce | 25 days disposable seedlings in the left and right sides of small (0.5cm) hypertrophy 99% 100% are little easily | Needed 95% 80-90% high-leveled and difficult in small very weak about 40 days | It is 67% 96% little difficult to need in big strong about 50 days | Big do not need in strong 60-90 days 45% 98% little easily |
Embodiment
The present invention is described in more detail below in conjunction with example:
Embodiment one
(1) the grow seedlings bed and the preparation of seedling growth greenhouse
A: direction inclined to one side 5 ° to the west of the bed of growing seedlings is made bed, and long 8 meters of seedbed is wide 2.7 meters, deep-cuts a trench in the bed, long 8 meters of ditch, and wide 30cm, dark 40cm, trench is divided into two equal little seedbeds with the seedbed, and each is wide 1.2 meters.
B: above the seedbed, take little shed (is material with the bamboo chip), require canopy high 12 meters, long 8 meters of canopy; And white plastic film (thickness is not limit), long 11 meters of film, two canopy mouths difference sagging 1.5 is in order to cover the canopy mouth.
C: river sand (thickness all can) or sandy soil are tiled on the little seedbed, and thickness is 5cm, requires sand bed soft behind the shop, and with 800 times of liquid of potassium permanganate sand bed is sterilized, and canopy mouth plastic foil is left behind covers the canopy mouth, and it is standby to raise plastic foil after 24 hours.
(2) preparation " GP clones fluid infusion "
A: the weight proportion of " GP clones fluid infusion " No. 1 each raw material of liquid is in this example:
IBA (indolebutyric acid) 1600mg
NAA (methyl) 250mg
6-BA (6-benzylaminopurine) 0.5mg
Distilled water adds to 1000ml
B: the weight proportion of " GP clones fluid infusion " No. 2 each raw material of liquid is in this example:
KNO
3(potassium nitrate) 1600mg
CaCl
22H
2O (calcium chloride) 400mg
Distilled water adds to 1000ml
C: the weight proportion of " GP clones fluid infusion " No. 3 each raw material of liquid is in this example:
NH
4NO
3(ammonium nitrate) 1450mg
MgSO
47H
2O (magnesium sulfate) 330mg
KH
2PO
4(potassium dihydrogen phosphate) 100mg
Distilled water adds to 1000ml
D: the weight proportion of " GP clones fluid infusion " No. 4 each raw material of liquid is in this example:
MnSO
44H
2O (manganese sulphate) 20mg
ZnSO
47H
2O (zinc sulphate) 11mg
H
3BO
3(boric acid) 10mg
Distilled water adds to 1000ml
E: the weight proportion of " GP clones fluid infusion " No. 5 each raw material of liquid is in this example:
Na
2MoO
42H
2(sodium molybdate) 0.25mg
CuSO
45H
2O (copper sulphate) 0.03mg
CoCl
26H
2O (cobalt chloride) 0.03mg
KI (potassium iodide) 1mg
Distilled water adds to 1000ml
F: the weight proportion of " GP clones fluid infusion " No. 6 each raw material of liquid is in this example:
FeSO
47H
2O (iron sulfate) 50mg
Na
2-EDTA (disodium ethylene diamine tetraacetate); 40mg
Distilled water adds to 1000ml
G: the weight proportion of " GP clones fluid infusion " No. 7 each raw material of liquid is in this example:
Vit.B6 (pyridoxine hydrochloride) 0.8mg
Gly (glycine) 2.6mg
Inositol 78mg
Coconut palm breast 125mg
Distilled water adds to 1000ml
H: the weight proportion of " GP clones fluid infusion " No. 8 each raw material of liquid is in this example:
C
2H
22O
11(sucrose) 16000mg
Distilled water adds to 1000ml
I: the weight proportion of " GP clones fluid infusion " No. 9 each raw material of liquid is in this example:
Hydrogen peroxide 100000mg
Distilled water adds to 1000ml
(3) female parent is bred the preparation of material
The branch (age of branch is not limit, but should not be thick excessively) of Taxus x media is collected fringe bar need not sterilize (this technology inoculation operating process implements to carry disease germs operation, does not need any sterilization) from elite stand with sharp blade or scissors.
The temperature and humidity that keeps the Taxus x media branch is cut into 0.5cm long stem section or stem apex with branch under the half-light condition, keep blade, and lower cut keeps flat mouthful.
(4) inoculation
Fast the stem section that cuts or the lower cut of stem apex material are dipped in " GP clones fluid infusion " No. 1 liquid middling speed, the speed time of dipping in is no more than 1 second, and it is 0.1cm that material is dipped the degree of depth.
Material after dipping is seeded on the husky bed in the treated little shed immediately, requires husky bed humidity to pinch not agglomerating being advisable with husky fist, the inoculation degree of depth is that the maternal material of the husky bed of lower cut contact is not advisable.Inoculum density is every square metre of 800-1000 strain.After inoculation finished, with seedbed and little shed spraying one time (this technology adopts the artificial sprayer of rod-pulling type manually to spray, with better adjusting spray intensity), intensity was advisable with the blade face No drip type with " GP clones fluid infusion " No. 9 liquid.
(5) postvaccinal management
Temperature:
Inoculate the seedling management Taxus x media 4-10 month, general temperature of shed was controlled at the 25-28 degree and was advisable daytime, regulate the moisture cooling if temperature is too high by spraying, if temperature is crossed the low method of envelope canopy mouth that then adopts and is heated, general front cover half canopy mouth in evening (promptly staying osculum), temperature of shed generally is controlled at the 15-20 degree, but will note ventilating.
Taxus x media is inoculated the seedling management November to March next year, and daytime, general temperature of shed was controlled at 12-16 degree (it is better that the ground hot line is laid in the seedbed), and temperature is crossed the low canopy that can seal and controlled temperature, but will ventilate at any time.General temperature of shed was controlled at the 8-12 degree and was advisable evening.
Humidity (moisture):
Moisture management mainly contains humidity in the canopy, inoculation seedling blade face humidity and seedbed moisture management.The inoculation of 4-10 part, humidity requirement wants big in the canopy, inoculation seedling humidity requirement keeps the very thin moisture film of one deck constantly, (the moisturizing instrument is the rod-pulling type sprayer to promptly dried i.e. benefit, method is artificial spraying, if adopt auto spraying or computer control spraying then not reach desirable effect, survival rate reduces), it is big that the seedbed humidity requirement is also wanted.All the other inoculation times, humidity requirement is little in the canopy, and inoculation seedling humidity also requires the moisture film that keeps one deck very thin constantly, promptly does promptly and mends, and the seedbed humidity requirement is less relatively.
Illumination:
Inoculate seedling 4-10 month and will carry out shading treatment daytime, can be with 75% shading net shading, available in case of necessity 2 layers of shading net (partial blade is arranged on the inoculation material, and suitable illumination can be carried out photosynthesis, generation part nutriment) when illumination is strong.All the other time inoculations can be carried out suitable shading or not shading according to actual conditions.Shading net is generally built 2 meters above seedling growth greenhouse.
Ventilate (oxygen and carbonic acid gas):
Ventilation is extremely important in seedling raising process, will open wide two ends canopy mouth when temperature is too high, often ventilates, and temperature is crossed when hanging down and also will be ventilated according to the actual conditions compartment.The commutative canopy wind outer air of ventilating, combustion gas strengthens oxygen and carbonic acid gas, increases the photosynthesis of Taxus x media, and can reduce the generation of disease.
Nutrient (nutrition):
Taxus x media will consume a large amount of nutriment in seedling raising process.In whole seedling raising process, prepared a cover fluid infusion of suitable Taxus x media for this reason.Concrete application program is as follows:
" GP clones fluid infusion " No. 9:
Time of application: 1st, 11,19,27,35 days
" GP clones fluid infusion " No. 2:
Time of application: 4th, 12,20,28 days
" GP clones fluid infusion " No. 3:
Time of application: 5th, 13,21,29 days
" GP clones fluid infusion " No. 6:
Time of application: 6th, 14,22,30 days
" GP clones fluid infusion " No. 7:
Time of application: 7th, 15,23,31 days
" GP clones fluid infusion " No. 8:
Time of application: 8th, 16,24,32 days
" GP clones fluid infusion " No. 4:
Time of application: 9th, 17,25,33 days
" GP clones fluid infusion " No. 5:
Time of application: 10th, 18,26,34 days
(6) take root, hardening and go out the garden
Through cultivating, in the time of the 25th day, the bed of growing seedlings promptly has the Taxus x media on 60% right side to take root, and during by the 30th day, rooting rate can reach 99%.30-35 days, alleviate spray intensity gradually, stop to use of fluid infusion in the time of the 36th day, little shed can be raised and carry out hardening, during by the 50th day, seedling has sent tender shoots, can go out the garden and transplant or carry out subculture and breed.
Example two
Every preparation in this example is all consistent with example one with the supporting technology management method, and different is the preparation raw material weight ratio difference of " GP clones fluid infusion ", and details is as follows:
A: the weight proportion of " GP clones fluid infusion " No. 1 each raw material of liquid is in this example:
IBA (indolebutyric acid) 1590mg
NAA (methyl) 248mg
6-BA (6-benzylaminopurine 0.48mg
Distilled water adds to 1000ml
B: the weight proportion of " GP clones fluid infusion " No. 2 each raw material of liquid is in this example:
KNO
3(potassium nitrate) 1590mg
CaCl
22H
2O (calcium chloride) 398mg
Distilled water adds to 1000ml
C: the weight proportion of " GP clones fluid infusion " No. 3 each raw material of liquid is in this example:
NH
4NO
3(ammonium nitrate) 1445mg
MgSO
47H
2O (magnesium sulfate) 326mg
KH
2PO
4(potassium dihydrogen phosphate) 98mg
Distilled water adds to 1000ml
D: the weight proportion of " GP clones fluid infusion " No. 4 each raw material of liquid is in this example:
MnSO
44H
2O (manganese sulphate) 19.8mg
ZnSO
47H
2O (zinc sulphate) 10.5mg
H
3BO
3(boric acid) 9.8mg
Distilled water adds to 1000ml
E: the weight proportion of " GP clones fluid infusion " No. 5 each raw material of liquid is in this example:
Na
2MoO
42H
2(sodium molybdate) 0.22mg
CuSO
45H
2O (copper sulphate) 0.028mg
CoCl
26H
2O (cobalt chloride) 0.026mg
KI (potassium iodide) 0.98mg
Distilled water adds to 1000ml
F: the weight proportion of " GP clones fluid infusion " No. 6 each raw material of liquid is in this example:
FeSO
47H
2O (iron sulfate) 49.5mg
Na
2-EDTA (disodium ethylene diamine tetraacetate); 39mg
Distilled water adds to 1000ml
G: the weight proportion of " GP clones fluid infusion " No. 7 each raw material of liquid is in this example:
Vit.B6 (pyridoxine hydrochloride) 0.79mg
Gly (glycine) 2.26mg
Inositol 76mg
Coconut palm breast 124.6mg
Distilled water adds to 1000ml
H: the weight proportion of " GP clones fluid infusion " No. 8 each raw material of liquid is in this example:
C
2H
22O
11(sucrose) 15940mg
Distilled water adds to 1000ml
I: the weight proportion of " GP clones fluid infusion " No. 9 each raw material of liquid is in this example:
Hydrogen peroxide 88500mg
Distilled water adds to 1000ml
Example three
Every preparation in this example is all consistent with example one with the supporting technology management method, and different is the preparation raw material weight ratio difference of " GP clones fluid infusion ", and details is as follows:
A: the weight proportion of " GP clones fluid infusion " No. 1 each raw material of liquid is in this example:
IBA (indolebutyric acid) 1610mg
NAA (methyl) 255mg
6-BA (6-benzylaminopurine) 0.45mg
Distilled water adds to 1000ml
B: the weight proportion of " GP clones fluid infusion " No. 2 each raw material of liquid is in this example:
KNO
3(potassium nitrate) 1610mg
CaCl
22H
2O (calcium chloride) 402mg
Distilled water adds to 1000ml
C: the weight proportion of " GP clones fluid infusion " No. 3 each raw material of liquid is in this example:
NH
4NO
3(ammonium nitrate) 1450mg
MgSO
47H
2O (magnesium sulfate) 332mg
KH
2PO
4(potassium dihydrogen phosphate) 105mg
Distilled water adds to 1000ml
D: the weight proportion of " GP clones fluid infusion " No. 4 each raw material of liquid is in this example:
MnSO
44H
2O (manganese sulphate) 21mg
ZnSO
47H
2O (zinc sulphate) 12mg
H
3BO
3(boric acid) 10mg
Distilled water adds to 1000ml
E: the weight proportion of " GP clones fluid infusion " No. 5 each raw material of liquid is in this example:
Na
2MoO
42H
2(sodium molybdate) 0.29mg
CuSO
45H
2O (copper sulphate) 0.03mg
CoCl
26H
2O (cobalt chloride) 0.033mg
KI (potassium iodide) 1mg
Distilled water adds to 1000ml
F: the weight proportion of " GP clones fluid infusion " No. 6 each raw material of liquid is in this example:
FeSO
47H
2O (iron sulfate) 52mg
Na
2-EDTA (disodium ethylene diamine tetraacetate) 40mg
Distilled water adds to 1000ml
G: the weight proportion of " GP clones fluid infusion " No. 7 each raw material of liquid is in this example:
Vit.B6 (pyridoxine hydrochloride) 0.8mg
Gly (glycine) 2.6mg
Inositol 78.5mg
Coconut palm breast 127.5mg
Distilled water adds to 1000ml
H: the weight proportion of " GP clones fluid infusion " No. 8 each raw material of liquid is in this example:
C
2H
22O
11(sucrose) 16050mg
Distilled water adds to 1000ml
I: the weight proportion of " GP clones fluid infusion " No. 9 each raw material of liquid is in this example:
Hydrogen peroxide 105000mg
Distilled water adds to 1000ml
For proving performance of the present invention, the inventor has carried out comprehensive experiment with first example performance, and the experiment situation is as follows:
1. experiment material: Taxus x media branch.
2. the material unit of providing: Chongqing Yuanda Ecological Agriculture Development Co.ltd.
3. experimental technique:
Experimental technique and all materials are prepared, step, and the fluid infusion preparation is all identical with first example, and experimental result is as follows:
Table one: the callus production is table as a result
| Various branch inoculation numbers (individual) | Callus generation rate in the time of 10 days | Callus generation rate in the time of 12 days | Callus generation rate in the time of 17 days | Callus generation rate in the time of 25 days |
| 1000 | ?32 | ?48 | ?76.5 | 100 |
Table two: the situation of taking root is table as a result
| Various branch inoculation numbers (individual) | The beginning rootage duration | Rooting rate in the time of the 25th day | Rooting rate in the time of the 30th day | Average every seedling rooting number (individual) |
| 1000 | The 15th day | 60 | ?99 | 24.6 |
Table two: germination is table as a result
| Various branch inoculation numbers (individual) | The beginning germinating time | Germination rate in the time of the 50th day | Average height of seedling (mm) in the time of the 50th day | Average seedling thick (mm) in the time of the 50th day |
| 1000 | The 27th day | 99 | ?5.9 | 2.3 |
4, experiment conclusion:
1. all available this inventive method breeding of the various branches of Taxus x media.
2. the present invention can make Taxus x media take root about 25 days, and rooting rate reaches 99% in 30 days.
3. the present invention can carry out the second generation and breeds fast in 50 days, and every strain seedling can expand numerous 8-10 strain.
4. the present invention can obtain a large amount of Taxus x media seedlings in the short period of time, and to protection China taxus resource, popularizing planting plays deciding factor.
5. the present invention is unidentical with existing Taxus x media propagation technique, is a brand-new Taxus x media propagation method, does not also have correlation technique to occur in China.
Claims (4)
1, a kind of Taxus x media method for quickly breeding is characterized in that carrying out as follows:
(1) the grow seedlings bed and the preparation of seedling growth greenhouse
A: direction inclined to one side 5 ° to the west of the bed of growing seedlings is made bed, and long 8 meters of seedbed is wide 2.7 meters, deep-cuts a trench in the bed, long 8 meters of ditch, and wide 30cm, dark 40cm, trench is divided into two equal little seedbeds with the seedbed, and each is wide 1.2 meters;
B: above the seedbed, take little shed, require canopy high 12 meters, long 8 meters of canopy with bamboo chip; And cover the white plastic film, and long 11 meters of film, two canopy mouths difference sagging 1.5 is in order to cover the canopy mouth;
C: river sand or sandy soil are tiled on the little seedbed, and thickness is 5cm, requires sand bed soft behind the shop, and with 800 times of liquid of potassium permanganate sand bed is sterilized, and canopy mouth plastic foil is left behind covers the canopy mouth, and it is standby to raise plastic foil after 24 hours;
(2) preparation is fit to a cover fluid infusion of Taxus x media growth
" GP clones fluid infusion " No. 1:
IBA (indolebutyric acid) 1540～1660mg
NAA (methyl) 242～265mg
6-BA (6-benzylaminopurine) 0.4～0.56mg
Distilled water adds to 1000ml
Main points: make mixed liquor according to concentration, with a spot of 95% alcohol dissolving IBA and NAA, after add an amount of water again.6-BA uses 1molL earlier
-1HCl dissolving, it is fixed molten to add water again.After preparation finishes, measure its pH value with acidometer, the pH value of this solution is 6.0, if meta-acid 1molL
-1NaOH regulates, if inclined to one side alkali 1molL
-1HCl regulates;
" GP clones fluid infusion " No. 2:
KNO
3(potassium nitrate) 1580～1620mg
CaCl
22H
2O (calcium chloride) 395～410mg
Distilled water adds to 1000ml;
" GP clones fluid infusion " No. 3:
NH
4NO
3(ammonium nitrate) 1440～1460mg
MgSO
47H
2O (magnesium sulfate) 325～335mg
KH
2PO
4(potassium dihydrogen phosphate) 96～110mg
Distilled water adds to 1000ml;
" GP clones fluid infusion " No. 4:
MnSO
44H
2O (manganese sulphate) 18.5～22.5mg
ZnSO
47H
2O (zinc sulphate) 10～12.5mg
H
3BO
3(boric acid) 9.6～11mg
Distilled water adds to 1000ml;
" GP clones fluid infusion " No. 5:
Na
2MoO
42H
2(sodium molybdate) 0.2～0.3mg
CuSO
45H
2O (copper sulphate) 0.025～0.035mg
CoCl
26H
2O (cobalt chloride) 0.025～0.035mg
KI (potassium iodide) 0.85～1.24mg
Distilled water adds to 1000ml;
" GP clones fluid infusion " No. 6:
FeSO
47H
2O (iron sulfate) 46～53mg
Na
2-EDTA (disodium ethylene diamine tetraacetate); 38.5～45mg
Distilled water adds to 1000ml;
" GP clones fluid infusion " No. 7:
Vit.B6 (pyridoxine hydrochloride) 0.78～0.85mg
Gly (glycine) 2.25～2.65mg
Inositol 75.5～80mg
Coconut palm breast 120～128mg
Distilled water adds to 1000ml;
" GP clones fluid infusion " No. 8:
C
2H
22O
11(sucrose) 15800～16150mg
Distilled water adds to 1000ml;
" GP clones fluid infusion " No. 9:
Hydrogen peroxide 85000～120000mg
Distilled water adds to 1000ml;
(3) female parent is bred the preparation of material
The branch of Taxus x media is collected from elite stand with sharp blade or scissors, and the fringe bar need not sterilized;
The temperature and humidity that keeps the Taxus x media branch is cut into stem section or stem apex long about 0.5cm with branch under the half-light condition, keep blade, and lower cut keeps flat mouthful;
(4) inoculation
The stem section that cuts or the lower cut of stem apex material are dipped in " GP clones fluid infusion " No. 1 liquid middling speed, and the speed time of dipping in is no more than 1 second, and it is 0.5～0.15cm that material is dipped the degree of depth;
Material after dipping is seeded on the husky bed in the treated little shed immediately, requires husky bed humidity to pinch not agglomerating being advisable with husky fist, the inoculation degree of depth is that the maternal material of the husky bed of lower cut contact is not advisable, and inoculum density is every square metre of 800-1000 strain; After inoculation finishes, with " GP clones fluid infusion " No. 9 liquid sprayed one time in seedbed and little shed, intensity is advisable with the blade face No drip type;
(5) postvaccinal management
Temperature:
Inoculate seedling management Taxus x media 4-10 month, general temperature of shed was controlled at the 25-28 degree and was advisable daytime, evening front cover part canopy mouth, temperature of shed is controlled at the 15-20 degree, guarantees good ventilation;
Taxus x media is inoculated the seedling management November to March next year, and daytime, temperature of shed was controlled at the 12-16 degree, and evening, temperature of shed was controlled at the 8-12 degree;
Humidity:
Moisture management mainly contains humidity in the canopy, inoculation seedling blade face humidity and seedbed moisture management; 4-10 month is inoculated, and inoculation seedling blade face humidity requirement keeps the very thin moisture film of one deck constantly, promptly does promptly and mends, the moisturizing instrument is the rod-pulling type sprayer, and method is artificial spraying, and this is the precondition that guarantees interior humidity of canopy and seedbed humidity, promptly this condition satisfies, and other corresponding conditions also can satisfy; Relatively season, 4-10 month is inoculated, and humidity requirement is bigger than all the other time inoculation desired moisture levels in the canopy, and the seedbed humidity requirement is also corresponding wants big, and management difficulty is also corresponding bigger;
All the other inoculations in season, management is easier to relatively, and it also is the prerequisite that keeps interior humidity of canopy and seedbed humidity that inoculation seedling blade face humidity requirement keeps the very thin moisture film of one deck constantly, just comparatively speaking, it is little that the interior humidity requirement of canopy is inoculated desired moisture level than 4-10 month, and the seedbed humidity requirement is also corresponding little;
Illumination:
Inoculate seedling 4-10 month and will carry out shading treatment daytime, can be with 75% shading net shading, available in case of necessity 2 layers of shading net when illumination is strong; All the other time inoculations can be carried out suitable shading or not shading according to actual conditions, and shading net is generally built 2 meters above seedling growth greenhouse;
Ventilate:
Will open wide two ends canopy mouth when temperature is too high, often ventilate, temperature is crossed when hanging down and also will be ventilated according to the actual conditions compartment;
Nutrient:
In whole seedling raising process, apply a cover fluid infusion that is fit to the Taxus x media growth stage by stage, concrete application program is as follows:
" GP clones fluid infusion " No. 9:
Time of application: 1st, 11,19,27,35 days
" GP clones fluid infusion " No. 2:
Time of application: 4th, 12,20,28 days
" GP clones fluid infusion " No. 3:
Time of application: 5th, 13,21,29 days
" GP clones fluid infusion " No. 6:
Time of application: 6th, 14,22,30 days
" GP clones fluid infusion " No. 7:
Time of application: 7th, 15,23,31 days
" GP clones fluid infusion " No. 8:
Time of application: 8th, 16,24,32 days
" GP clones fluid infusion " No. 4:
Time of application: 9th, 17,25,33 days
" GP clones fluid infusion " No. 5:
Time of application: 10th, 18,26,34 days
Illustrate:
" GP clones fluid infusion " is spray application 2-9 number, is exclusively used in the replenishing of mineral element of Taxus x media; Shared fluid infusion is 35 days in the seedling raising process, wherein 2-3 days need not any fluid infusion, fluid infusion every day time of application is last spray time, spray intensity is advisable with the blade No drip type;
(6) take root, hardening and go out the garden
Through cultivating, in the time of the 25th day, the bed of growing seedlings promptly has the Taxus x media about 60% to take root, during by the 30th day, rooting rate can reach 99%, 30-35 days, alleviate spray intensity gradually, stop to use of fluid infusion in the time of the 36th day, little shed can be raised and carry out hardening, during by the 50th day, seedling has sent tender shoots, can go out the garden and transplant or carry out subculture and breed.
2, according to the described Taxus x media method for quickly breeding of claim 1, it is characterized in that:
A: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 1 liquid:
IBA (indolebutyric acid) 1585～1610mg
NAA (methyl) 248～255mg
6-BA (6-benzylaminopurine) 0.45～0.53mg
Distilled water adds to 1000ml;
B: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 2 liquid:
KNO
3(potassium nitrate) 1590～1610mg
CaCl
22H
2O (calcium chloride) 398～405mg
Distilled water adds to 1000ml;
C: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 3 liquid:
NH
4NO
3(ammonium nitrate) 1445～1455mg
MgSO
47H
2O (magnesium sulfate) 326～332mg
KH
2PO
4(potassium dihydrogen phosphate) 98～105mg
Distilled water adds to 1000ml;
D: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 4 liquid:
MnSO
44H
2O (manganese sulphate) 19.5～21.5mg
ZnSO
47H
2O (zinc sulphate) 10.512mg
H
3BO
3(boric acid) 9.8～10.05mg
Distilled water adds to 1000ml;
E: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 5 liquid:
Na
2MoO
42H
2(sodium molybdate) 0.22～0.29mg
CuSO
45H
2O (copper sulphate) 0.026～0.032mg
CoCl
26H
2O (cobalt chloride) 0.026～0.034mg
KI (potassium iodide) 0.95～1.04mg
Distilled water adds to 1000ml;
F: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 6 liquid:
FeSO
47H
2O (iron sulfate) 48～52.5mg
Na
2-EDTA (disodium ethylene diamine tetraacetate); 39～44mg
Distilled water adds to 1000ml;
G: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 7 liquid:
Vit.B6 (pyridoxine hydrochloride) 0.79～0.82mg
Gly (glycine) 2.26～2.62mg
Inositol 76～78.5mg
Coconut palm breast 124～127.5mg
Distilled water adds to 1000ml;
H: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 8 liquid:
C
2H
22O
11(sucrose) 15940～16050mg
Distilled water adds to 1000ml;
I: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 9 liquid:
Hydrogen peroxide 88000～110000mg
Distilled water adds to 1000ml.
3, according to the described Taxus x media method for quickly breeding of claim 1, it is characterized in that:
A: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 1 liquid:
IBA (indolebutyric acid) 1600mg
NAA (methyl) 250mg
6-BA (6-benzylaminopurine) 0.5mg
Distilled water adds to 1000ml;
B: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 2 liquid:
KNO
3(potassium nitrate) 1600mg
CaCl
22H
2O (calcium chloride) 400mg
Distilled water adds to 1000ml;
C: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 3 liquid:
NH
4NO
3(ammonium nitrate) 1450mg
MgSO
47H
2O (magnesium sulfate) 330mg
KH
2PO
4(potassium dihydrogen phosphate) 100mg
Distilled water adds to 1000ml;
D: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 4 liquid:
MnSO
44H
2O (manganese sulphate) 20mg
ZnSO
47H
2O (zinc sulphate) 11mg
H
3BO
3(boric acid) 10mg
Distilled water adds to 1000ml;
E: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 5 liquid:
Na
2MoO
42H
2(sodium molybdate) 0.25mg
CuSO
45H
2O (copper sulphate) 0.03mg
CoCl
26H
2O (cobalt chloride) 0.03mg
KI (potassium iodide) 1mg
Distilled water adds to 1000ml;
F: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 6 liquid:
FeSO
47H
2O (iron sulfate) 50mg
Na
2-EDTA (disodium ethylene diamine tetraacetate); 40mg
Distilled water adds to 1000ml;
G: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 7 liquid:
Vit.B6 (pyridoxine hydrochloride) 0.8mg
Gly (glycine) 2.6mg
Inositol 78mg
Coconut palm breast 125mg
Distilled water adds to 1000ml;
H: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 8 liquid:
C
2H
22O
11(sucrose) 16000mg
Distilled water adds to 1000ml;
I: the weight proportion of each raw material is in said " GP clones fluid infusion " No. 9 liquid:
Hydrogen peroxide 100000mg
Distilled water adds to 1000ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03117884 CN1220423C (en) | 2003-05-17 | 2003-05-17 | Quick taxus mandia propagating method |
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|---|---|---|---|
| CN 03117884 CN1220423C (en) | 2003-05-17 | 2003-05-17 | Quick taxus mandia propagating method |
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| CN1220423C true CN1220423C (en) | 2005-09-28 |
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| CN1319452C (en) * | 2005-12-06 | 2007-06-06 | 西南大学 | Efficient water soluble root-inducing powcler for taxus media |
| CN102792831B (en) * | 2011-05-26 | 2015-07-15 | 汉中隆康生物科技开发有限公司 | High-efficiency rapid propagation technique for Chinese yew |
| CN102301901B (en) * | 2011-07-29 | 2012-11-14 | 云南省农业科学院花卉研究所 | Sequoia sempervirens seed seedling culture method |
| CN102726202B (en) * | 2012-07-19 | 2014-01-08 | 湖南省森林植物园 | Method for rapidly propagating taxus chinensis seedlings |
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| CN103931393B (en) * | 2014-04-11 | 2016-02-24 | 桂林市林业科学研究所 | A kind of method utilizing waste material of edible mushroom to cultivate Taxus x media bag seedling |
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| CN104956880B (en) * | 2015-06-15 | 2017-08-25 | 孝感市大自在生态农业发展有限公司 | A kind of quick Taxus mandia propagating method |
| CN105123379B (en) * | 2015-07-20 | 2017-07-14 | 重庆紫逸农业发展有限公司 | Taxus x media fast breeding method |
| CN105210827B (en) * | 2015-09-30 | 2017-12-26 | 马维成 | Phase in early spring Yew cuttage method for culturing seedlings |
| CN105248085A (en) * | 2015-10-08 | 2016-01-20 | 李正 | Cultivation method of Chinese yews |
| CN105248228A (en) * | 2015-11-13 | 2016-01-20 | 周福进 | Taxus media cultivation method |
| CN105432297B (en) * | 2015-12-03 | 2018-06-29 | 宜宾云辰乔木园林有限责任公司 | A kind of method for promoting Taxus x media cuttage root-taking |
| CN106718914A (en) * | 2016-12-23 | 2017-05-31 | 叶宗耀 | A kind of Chinese yew culture medium |
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