CN103749126A - Sustainable three-nerved spicebush cultivation method - Google Patents
Sustainable three-nerved spicebush cultivation method Download PDFInfo
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Abstract
The invention relates to a sustainable three-nerved spicebush cultivation method aiming to fill the gap in the field at preset. The sustainable three-nerved spicebush cultivation method is characterized by including the steps of (1) selecting three-nerved spicebush plants which are triennial or above, cutting off two-thirds of the part of the plants on the ground in autumn or winter, (2) digging into soil at the roots of the plants, lifting the overall plants, clipping the inflated root tubes, (3) pruning all roots until the length thereof is 8-12 times of the ground diameter, (4) soaking cuts left after root pruning into 1% of calcium hydroxide solution for 5 seconds, keeping the cuts below the level of the calcium hydroxide solution and keeping the distance between the cuts and the level within 5 mm during soaking, and then drying the same in air, (5), mixing 0.1% of rooting powder and 1% of Lachnum fermentation liquor with the dug soil, (6) replanting the three-nerved spicebush into the dug hole and then covering it with soil, (7) watering in time, and (8) performing root tube harvesting three years later. The sustainable three-nerved spicebush cultivation method can be applied sustainably and is an environmental friendly method.
Description
Technical field
The present invention relates to a kind of cultivation method, especially relate to the sustainable cultivation method of a kind of root of three-nerved spicebush, can obtain continuously for a long time root of three-nerved spicebush piece root.
Background technology
The root of three-nerved spicebush (
lindera aggregata(Sims) be Kosterm.) Lauraceae, Lindera plant, be born in height above sea level 200-1000 rice and face south in hillside fields, mountain valley or sparse woods shrubbery.Produce the provinces and regions such as Zhejiang, Jiangxi, Fujian, Anhui, Hunan, Sichuan, Guangdong, Guangxi, Shaanxi, Taiwan, be adapted at sunnyly, on the soil is porous fertile acid ground, grow.< < Compendium of Materia Medica > >, < < Kaibao Bencao > >, < < Chinese medicine voluminous dictionary > >, < < Bencao Jingshu > > and < < book on Chinese herbal medicine meet in the ethnopharmacology classical works such as source > > all on the books, it has loose cold, pain relieving, bowl spares, pleasant, open strongly fragrant, increase enterogastric peristalsis, excited cerebral cortex, accelerate blood circulation, hemostasis, the effect such as antibacterial.
The root of three-nerved spicebush be take wild excavating as main at present, because the root of three-nerved spicebush is used as medicine with piece root, its taproot can not hyoscine, and the field root of three-nerved spicebush of gathering is all that to take the death of whole strain plant be cost substantially, root of three-nerved spicebush strain abandons after piece root is gathered in excavation, can cause the problems such as environmental disruption and water and soil loss again.
Also there is no at present the sustainable cultivation method of a kind of root of three-nerved spicebush, if open day is on 08 28th, 2013, publication number is in the Chinese patent of CN103262736A, discloses a kind of Radix Codonopsis cultivation method of efficient volume increase, and this cultivation method comprises the steps: to train seedling front soil preparation, seed preparation; Sowing; Rapid seedling cultivation a, employing first generation group training seedling, as propagating materials; B, when seedbed seedling grows to 25 centimetres high, stem apex, stem section form, just can start to get its stem apex, stem section, wound is immersed 500ppm rooting powder solution and is disposed, directly inoculate agricultural facility booth hotbed, stem apex stem section starts to survive regeneration in hotbed soil, 28～35 ℃ of growths of temperature 30 days, forms second generation whole plant; After c, second generation whole plant form, continue to get its plants stems section and stem apex and in agricultural facility booth seedbed, continue inoculation reproduction, after 30 days, third generation plant seedling forms, and 30 days cycles can emerge for planting; Autumn sowing is advisable to transplant autumn, adopts ridge to plant or ridge cultivation, and this cultivation method is not suitable for the cultivation root of three-nerved spicebush.
Summary of the invention
The object of the invention is to overcome traditional root of three-nerved spicebush deficiencies such as the wasting of resources of middle existence and environmental disruption of gathering, and provide a kind of can sustainable utilization, the environmentally friendly sustainable cultivation method of the root of three-nerved spicebush.
The present invention addresses the above problem adopted technical scheme: the feature of the sustainable cultivation method of this root of three-nerved spicebush is: comprise the steps:
(1) select the above wild or cultivation root of three-nerved spicebush strain of life in 3 years, spring or autumn, cut off the branch of acrial part 2/3rds, obtain the beta pruning root of three-nerved spicebush; This step is selected the longer root of three-nerved spicebush strain of growth time as far as possible, at least take life in 3 years, according to inventor's research, the above root of three-nerved spicebush of life in 5 years preferably, 2/3rds acrial part is pruned away, and after preventing from again planting back, root of three-nerved spicebush transpiration is excessive, dehydration is dead, also facilitates gathering and heavily planting of next step.
(2) excavate the soil of beta pruning root of three-nerved spicebush root, the whole strain of the beta pruning root of three-nerved spicebush is mentioned, with scissors clip beta pruning root of three-nerved spicebush root, expand piece root; This step is the root of three-nerved spicebush piece root of gathering up to standard, notes carefully excavating root of three-nerved spicebush root soil, and the piece root that do not make to expand is retained in soil.
(3) 8-12 that is leading thread by all root prunings of the beta pruning root of three-nerved spicebush to length doubly; This step 1 is in order to cultivate conveniently, long so as not to root of three-nerved spicebush root, and in cave, bending is unfavorable for growth, the 2nd, all are all cut short, and be conducive to growing and the formation of piece root of the new root of the root of three-nerved spicebush.
(4) to be immersed in mass percent be 5 seconds in 1% calcium hydroxide aqueous solution to the wound after beta pruning root of three-nerved spicebush root is pruned, and after taking out, dries, and when wound soaks, the liquid level of calcium hydroxide aqueous solution covers wound, and the distance between liquid level and wound is less than 5mm; Adopt the calcium hydroxide aqueous solution (limewash) of low concentration to process root of three-nerved spicebush root wound, prevent that saprophytic bacteria from infecting, method is simple, and raw material is easy to get, and it is convenient to process.Calcium hydroxide aqueous solution is few using as far as possible, because according to inventor's research, have in the root of three-nerved spicebush growing environment of piece root, soil pH value is all in 4.6 ± 1.0 scopes, more than so low-concentration hydrogen calcium oxide aqueous solution does not cover wound 5mm, both play sterilization functions, and can not have influence on again the acid-base value of soil.
(5) soil excavating in step (2) being admixed to mass percent is the Lachnum zymotic fluid that 0.1% root-inducing powder and mass percent are 1%; Root-inducing powder plays rooting efficiency to the root of three-nerved spicebush, Lachnum is the endogenetic fungus of root of three-nerved spicebush piece root, closely related with the generation of secondary metabolites in piece root, and can stimulate the growth of root of three-nerved spicebush lateral root, be conducive to the formation of root of three-nerved spicebush piece root, in addition soil is processed, played the effect of loosening the soil, be conducive to the growth of root of three-nerved spicebush root and the growth of plant.
(6) root of three-nerved spicebush strain of handling well in step (4) is planted again in the cave that back cutting opens, and cover the soil that step (5) was processed; The root of three-nerved spicebush of beta pruning, the piece root of gathering is planted back in original cave again to ridging, covering.
(7) root of three-nerved spicebush strain that Xiang Chongzhi is good is watered in time; Cut the root of three-nerved spicebush of again planting back after root, except timely pouring normal root water, also need often to water; The gather spring root of three-nerved spicebush of replanting, shelters from heat or light to autumn with the shade net scaffolding of 50% light transmission capacity, and Second Year is without sheltering from heat or light, the root of three-nerved spicebush of replanting gather autumn without sheltering from heat or light, be grown in the wild root of three-nerved spicebush in forest, or the bionic cultivation root of three-nerved spicebush in mixed forest, forest canopy density is more than 0.5, also without sheltering from heat or light.The root of three-nerved spicebush of gathering after replanting, the particularly wild root of three-nerved spicebush and the bionic cultivation root of three-nerved spicebush, First Year will water in time, and Second Year is later without management.
(8) root of three-nerved spicebush strain of cultivation again, repeats piece root in 3 years later and gathers.The gather root of three-nerved spicebush strain of replanting, the piece root again bearing for the 3rd year can be gathered, but amount of growth is less, and the suitableeest is to gather once for 5 years.
Make thus the root of three-nerved spicebush strain in the present invention can sustainable utilization, shortened the root of three-nerved spicebush piece root growth cycle, be conducive to protection of the environment.
As preferably, the root of three-nerved spicebush strain in step of the present invention (1) is the above root of three-nerved spicebush of life in 5 years.
As preferably, in step of the present invention (3), by all root prunings of the beta pruning root of three-nerved spicebush to length, be leading thread 10 times.
As preferably, the root-inducing powder in step of the present invention (5) is ABT1 root-inducing powder.ABT1 root-inducing powder is the most obvious to root of three-nerved spicebush rooting efficiency,
As preferably, in step of the present invention (7), the root of three-nerved spicebush strain of heavily having planted is sheltered from heat or light with the shade net scaffolding of light transmission capacity 50%.
As preferably, in step of the present invention (8), every 5 years Repeated Harvesting piece roots once.
The present invention compared with prior art, has the following advantages and effect: (1) root of three-nerved spicebush plant can reuse, and the root of three-nerved spicebush piece root of gathering above for 3 years a time, has shortened the root of three-nerved spicebush piece root growth cycle; (2) avoided the gather shortcoming of pattern of traditional root of three-nerved spicebush, comprise root of three-nerved spicebush piece root gather after whole strain abandon the wasting of resources causing, and environmental disruption problems such as water and soil loss that cause after root of three-nerved spicebush death; (3) utilize the wound after calcium hydroxide aqueous solution is sheared piece root to carry out immersion treatment, prevent the dip-dye destruction of root-rot bacterium to wound; (4) utilize root-inducing powder and root of three-nerved spicebush endogenetic fungus Lachnum zymotic fluid to process soil, root-inducing powder and the acting in conjunction of Lachnum zymotic fluid, can effectively promote the growth of root of three-nerved spicebush piece root, stimulate the secretion of secondary metabolite in root of three-nerved spicebush piece root, can improve root of three-nerved spicebush piece root output and quality.
Embodiment
Below by embodiment, the present invention is described in further detail, and following examples are explanation of the invention and the present invention is not limited to following examples.
Embodiment 1.
The sustainable cultivation method of the root of three-nerved spicebush in the present embodiment comprises the steps.
(1) select the triennial wild root of three-nerved spicebush, the about 0.5cm of leading thread, cuts off the branch of acrial part 2/3 April in spring.
(2) excavate the soil of beta pruning root of three-nerved spicebush root, whole strain is mentioned, with scissors by root swelling piece root clip, approximately 3.7 grams of the piece roots that clip gets off.
(3) by all root prunings of the root of three-nerved spicebush to length, be 5cm.
(4) root being pruned to wound, to be immersed in mass percent be 5 seconds in 1% calcium hydroxide aqueous solution, after taking out, dries, more than while noticing that wound soaks, calcium hydroxide aqueous solution does not cover wound 5mm.
(5) soil excavating being admixed to mass percent is the Lachnum zymotic fluid that 0.1% ABT1 root-inducing powder and mass percent are 1%.
(6) root of three-nerved spicebush strain of step (4) being handled well is planted in the cave that back cutting opens again, and covers the soil that upper step (5) was processed.
(7) by the root of three-nerved spicebush strain of heavily having planted, with the shade net scaffolding of light transmission capacity 50%, shelter from heat or light, watered the same day and irrigated, within the 7th day, water once, water thereafter water spacer increases by two days at every turn, has the time of raining to postpone, to October without rewatering.
After (8) three years, carry out again above-mentioned root and gather, approximately 1.5 grams of the piece roots of gathering.
The sustainable cultivation method of the root of three-nerved spicebush in the present embodiment repeats above-mentioned steps (1) to step (8), and belonging to can sustainable utilization, environmentally friendly cultivation method.
Embodiment 2:
The sustainable cultivation method of the root of three-nerved spicebush in the present embodiment comprises the steps.
(1) in the wealthy mixed forest of pine of crown density 0.6, select the bionic cultivation root of three-nerved spicebush 100 strains of life in 6 years, the about 1.0cm of leading thread, cuts off the branch of acrial part 2/3 April in spring.
(2) excavate the soil of beta pruning root of three-nerved spicebush root, whole strain is mentioned, with scissors by root swelling piece root clip, approximately 31.1 ± 3.7 grams of the piece roots that every strain clip gets off.
(3) by all root prunings of the root of three-nerved spicebush to length, be 10cm.
(4) root being pruned to wound, to be immersed in mass percent be 5 seconds in 1% calcium hydroxide aqueous solution, after taking out, dries, more than while noticing that wound soaks, calcium hydroxide aqueous solution does not cover wound 5mm.
(5) soil excavating being admixed to mass percent is the Lachnum zymotic fluid that 0.1% ABT1 root-inducing powder and mass percent are 1%.
(6) root of three-nerved spicebush strain of step (4) being handled well is planted in the cave that back cutting opens again, and covers the soil that upper step (5) was processed.
(7) root of three-nerved spicebush strain of heavily having planted, without the processing of sheltering from heat or light, watered and irrigated the same day, within the 7th day, watered once, and water thereafter water spacer increases by two days at every turn, had the time of raining to postpone, to October without rewatering.
After (8) three years, select 50 strains to carry out above-mentioned root and gather, approximately 12.7 ± 3.8 grams of the piece roots that every strain is gathered; After 5 years, other 50 strains are carried out to above-mentioned root again and gather, approximately 24.0 ± 6.6 grams of the piece roots that every strain is gathered.
The sustainable cultivation method of the root of three-nerved spicebush in the present embodiment repeats above-mentioned steps (1) to step (8), and belonging to can sustainable utilization, environmentally friendly cultivation method.
Embodiment 3.
The sustainable cultivation method of the root of three-nerved spicebush in the present embodiment comprises the steps.
(1) select the above wild or cultivation root of three-nerved spicebush strain of life in 3 years, spring or autumn, cut off the branch of acrial part 2/3rds, obtain the beta pruning root of three-nerved spicebush.
(2) excavate the soil of beta pruning root of three-nerved spicebush root, the whole strain of the beta pruning root of three-nerved spicebush is mentioned, with scissors clip beta pruning root of three-nerved spicebush root, expand piece root.
(3) 8-12 that is leading thread by all root prunings of the beta pruning root of three-nerved spicebush to length doubly.
(4) to be immersed in mass percent be 5 seconds in 1% calcium hydroxide aqueous solution to the wound after beta pruning root of three-nerved spicebush root is pruned, and after taking out, dries, and when wound soaks, the liquid level of calcium hydroxide aqueous solution covers wound, and the distance between liquid level and wound is less than 5mm.
(5) soil excavating in step (2) being admixed to mass percent is the Lachnum zymotic fluid that 0.1% root-inducing powder and mass percent are 1%, and root-inducing powder is soil quality 0.1%, and Lachnum zymotic fluid is soil quality 1%.
(6) root of three-nerved spicebush strain of handling well in step (4) is planted again in the cave that back cutting opens, and cover the soil that step (5) was processed.
(7) root of three-nerved spicebush strain that Xiang Chongzhi is good is watered in time.
(8) root of three-nerved spicebush strain of cultivation again, repeats piece root in 3 years later and gathers.
The sustainable cultivation method of the root of three-nerved spicebush in the present embodiment repeats above-mentioned steps (1) to step (8), and belonging to can sustainable utilization, environmentally friendly cultivation method.
Embodiment 4.
The sustainable cultivation method of the root of three-nerved spicebush in the present embodiment comprises the steps.
(1) select the above wild or cultivation root of three-nerved spicebush strain of life in 5 years, spring or autumn, cut off the branch of acrial part 2/3rds, obtain the beta pruning root of three-nerved spicebush.Root of three-nerved spicebush strain in the present invention can be also the above root of three-nerved spicebush of life in 3 years.
(2) excavate the soil of beta pruning root of three-nerved spicebush root, the whole strain of the beta pruning root of three-nerved spicebush is mentioned, with scissors clip beta pruning root of three-nerved spicebush root, expand piece root.
(3) 8-12 that is leading thread by all root prunings of the beta pruning root of three-nerved spicebush to length doubly.General is leading thread by all root prunings of the beta pruning root of three-nerved spicebush to length 10 times.
(4) to be immersed in mass percent be 5 seconds in 1% calcium hydroxide aqueous solution to the wound after beta pruning root of three-nerved spicebush root is pruned, and after taking out, dries, and when wound soaks, the liquid level of calcium hydroxide aqueous solution and the distance between wound are less than 5mm.
(5) soil excavating in step (2) being admixed to mass percent is the Lachnum zymotic fluid that 0.1% root-inducing powder and mass percent are 1%.Root-inducing powder is preferably ABT1 root-inducing powder.
(6) root of three-nerved spicebush strain of handling well in step (4) is planted again in the cave that back cutting opens, and cover the soil that step (5) was processed.
(7) root of three-nerved spicebush strain of heavily having planted is sheltered from heat or light with the shade net scaffolding of light transmission capacity 50%, and water in time.
(8) root of three-nerved spicebush strain of cultivation again, repeats piece root in 3 years later and gathers, and is preferably every 5 years Repeated Harvesting piece roots once.
The sustainable cultivation method of the root of three-nerved spicebush in the present embodiment repeats above-mentioned steps (1) to step (8), and belonging to can sustainable utilization, environmentally friendly cultivation method.
Below by the mode of testing, the present invention is described further, and result of study is as follows.
A, the experiment of root of three-nerved spicebush branch cutting, somatotropin contrast.
Gather 3 years raw root of three-nerved spicebush seedling branches, be cut into 10 centimeter length, lower cut is horse ear shape, sheared-off part blade soaks in treatment fluid, be divided into ABT root-inducing powder No. 1, No. 2, ABT root-inducing powder, naa (NAA), indolebutyric acid (IBA) and contrast (CK) 5 processing, concentration is 100PPM, soak base portion 12h, soaking depth 2cm, then cuttage.
Result shows, No. 1, ABT root-inducing powder is best to root of three-nerved spicebush cuttage branch rooting efficiency.
B, endogenetic fungus experiment.
Inventor utilizes sterile water and ethanol, liquor natrii hypochloritis successively to the sterilizing of root of three-nerved spicebush piece root, cultivates, and purifying, increases bacterium and cultivate, the mycelium that obtains root of three-nerved spicebush endogenetic fungus, extracts its DNA, through rDNA-ITS, increases, order-checking, identifies, identifying root of three-nerved spicebush endogenetic fungus is Lachnum.The Lachnum of acquisition is cultivated with shaking table, be mixed with 1% strong microbial inoculum mud, when transplanting, take and do not dip in the direct cuttage seeding of transplanting of microbial inoculum as contrast (CK), compare.
Result shows, the result difference of two kinds of processing methods is (P<0.01) extremely significantly, and with the processing of microbial inoculum, lateral root number is not obviously more than with the processing of microbial inoculum, and root of three-nerved spicebush piece root mainly grows in lateral root, so processing, microbial inoculum can effectively increase survival rate and the piece root growth rate of transplanting.
C, following table gather after piece root and again plant back in original cave six groups of all ages and classes stage roots of three-nerved spicebush, secondary recovery piece root after 3 years, the contrast of twice amount of gathering for inventor.
Although the present invention with embodiment openly as above; but it is not in order to limit protection scope of the present invention; any technical staff who is familiar with this technology, not departing from change and the retouching of doing in the spirit and scope of the present invention, all should belong to protection scope of the present invention.
Claims (6)
1. the sustainable cultivation method of the root of three-nerved spicebush, is characterized in that: comprise the steps:
(1) select the above wild or cultivation root of three-nerved spicebush strain of life in 3 years, spring or autumn, cut off the branch of acrial part 2/3rds, obtain the beta pruning root of three-nerved spicebush;
(2) excavate the soil of beta pruning root of three-nerved spicebush root, the whole strain of the beta pruning root of three-nerved spicebush is mentioned, with scissors clip beta pruning root of three-nerved spicebush root, expand piece root;
(3) 8-12 that is leading thread by all root prunings of the beta pruning root of three-nerved spicebush to length doubly;
(4) to be immersed in mass percent be 5 seconds in 1% calcium hydroxide aqueous solution to the wound after beta pruning root of three-nerved spicebush root is pruned, and after taking out, dries, and when wound soaks, the liquid level of calcium hydroxide aqueous solution covers wound, and the distance between liquid level and wound is less than 5mm;
(5) soil excavating in step (2) being admixed to mass percent is the Lachnum zymotic fluid that 0.1% root-inducing powder and mass percent are 1%;
(6) root of three-nerved spicebush strain of handling well in step (4) is planted again in the cave that back cutting opens, and cover the soil that step (5) was processed;
(7) root of three-nerved spicebush strain that Xiang Chongzhi is good is watered in time;
(8) root of three-nerved spicebush strain of cultivation again, repeats piece root in 3 years later and gathers.
2. the sustainable cultivation method of the root of three-nerved spicebush according to claim 1, is characterized in that: the root of three-nerved spicebush strain in described step (1) is the above root of three-nerved spicebush of life in 5 years.
3. the sustainable cultivation method of the root of three-nerved spicebush according to claim 1, is characterized in that: in described step (3), be leading thread 10 times by all root prunings of the beta pruning root of three-nerved spicebush to length.
4. the sustainable cultivation method of the root of three-nerved spicebush according to claim 1, is characterized in that: the root-inducing powder in described step (5) is ABT1 root-inducing powder.
5. the sustainable cultivation method of the root of three-nerved spicebush according to claim 1, is characterized in that: in described step (7), the root of three-nerved spicebush strain of heavily having planted is sheltered from heat or light with the shade net scaffolding of light transmission capacity 50%.
6. the sustainable cultivation method of the root of three-nerved spicebush according to claim 1, is characterized in that: in described step (8), every 5 years Repeated Harvesting piece roots once.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106358683A (en) * | 2016-08-29 | 2017-02-01 | 青川县蜀道地中药材发展有限公司 | Standard culturing method of Qingchuan lindera aggregata |
| CN106912349A (en) * | 2017-03-01 | 2017-07-04 | 济南大学 | A kind of method that utilization fungi promotes galuteolin accumulation in In Flower Buds of Lonicera Japonica Thunb |
| CN112535080A (en) * | 2020-12-10 | 2021-03-23 | 浙大宁波理工学院 | Method for improving formation rate of fusiform tuberous roots for combined spicebush root medicines |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487687A (en) * | 2011-12-01 | 2012-06-13 | 广州康和药业有限公司 | Method for planting flaccid anemone rhizome in industrialized form and application thereof |
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2014
- 2014-01-09 CN CN201410009909.3A patent/CN103749126B/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106358683A (en) * | 2016-08-29 | 2017-02-01 | 青川县蜀道地中药材发展有限公司 | Standard culturing method of Qingchuan lindera aggregata |
| CN106912349A (en) * | 2017-03-01 | 2017-07-04 | 济南大学 | A kind of method that utilization fungi promotes galuteolin accumulation in In Flower Buds of Lonicera Japonica Thunb |
| CN106912349B (en) * | 2017-03-01 | 2020-03-13 | 济南大学 | Method for promoting accumulation of luteoloside in honeysuckle flower buds by using fungi |
| CN112535080A (en) * | 2020-12-10 | 2021-03-23 | 浙大宁波理工学院 | Method for improving formation rate of fusiform tuberous roots for combined spicebush root medicines |
| CN112535080B (en) * | 2020-12-10 | 2022-04-08 | 浙大宁波理工学院 | Method for improving formation rate of fusiform tuberous roots for combined spicebush root medicines |
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