CN106973685A - A kind of method for rapidly and efficiently breeding giant knotweed seedling - Google Patents
A kind of method for rapidly and efficiently breeding giant knotweed seedling Download PDFInfo
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- CN106973685A CN106973685A CN201710271616.6A CN201710271616A CN106973685A CN 106973685 A CN106973685 A CN 106973685A CN 201710271616 A CN201710271616 A CN 201710271616A CN 106973685 A CN106973685 A CN 106973685A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The present invention relates to plant breeding technical field, and in particular to a kind of method for rapidly and efficiently breeding giant knotweed seedling.The present invention includes a, press strip selection of time in annual 5~June, and breeding and seedling environment ventilation is ventilative, irrigation and drainage are convenient, shading rate 70~80%, control relative humidity 75~85%；The top stipes of b, straight snips with terminal bud does cuttage cutting, dips in the mud containing root-growing agent, insertion river sand matrix, obtains the cuttage seeding with terminal bud；C, the remaining giant knotweed plant secateurs trimming blade for cutting off the top stipes with terminal bud, leave and take 3~5 leaves close to top, to top otch sterilization, mound obtains press strip plant；D, treat the cuttage seeding with terminal bud and press strip plant leaf scar position adventitious root generation, now the cuttage seeding with terminal bud can do seedling and be transplanted, and produce.Cuttage is combined by the propagation method of giant knotweed of the present invention with mound layering method, and top stipes cuttage survival rate is up to 97%, and no base of leaf section Rooting percent is up to 93%, root of hair stipes survival rate 100%.
Description
Technical field
The present invention relates to plant breeding technical field, and in particular to a kind of method for rapidly and efficiently breeding giant knotweed seedling.
Background technology
Giant knotweed is polygonaceae plant giant knotweedPolygonum cuspidatumSieb. et Zucc. rhizome and root, property is micro-
Hardship, is slightly cold, with expelling wind and removing dampness, scattered stasis pain, relieving cough and reducing sputum effect, be clinically mainly used in joint pain, jaundice with damp-heat pathogen,
The treatment of the diseases such as Amenorrhea, coughing with a lot of sputum, scald, injury from falling down, carbuncle sore tumefacting virus, its principle active component is polygonin, in vain
Veratryl alcohol, rheum emodin etc..In recent years, due to excessively excavation, giant knotweed wild resource subject to severe risks of damage.Artificial cultivation, which turns into, to be solved
One of giant knotweed resource exhaustion problem, the effective way for meeting the market demand.Giant knotweed has abundant genetic diversity, economic characters
Wait further investigation.
The Chinese invention patent of Publication No. " CN104885933A " discloses a kind of cultural method of giant knotweed seedling, and it is used
Sterile bud culture：Using giant knotweed tender stem or tender shoots as explant, through alcohol disinfecting, (0.05%~0.15%) HgCl2After sterilizing,
Sterile water wash, it is seeded in 1/2MS or MS and adds culture on the culture medium of appropriate auxin to obtain sterile bud in 12~18 days；
Adventitious shoots culture：Clip grows sterile bud, is inoculated into the adventitious shoots culture base for adding appropriate auxin to prepare with 1/2MS
Upper culture；Strong sprout, culture of rootage：Multiple Buds grow to that 1cm or so is long to be inoculated into 1/2MS and/or MS and add appropriate auxin
Strong sprout, sub-bottle culture, 15~30 DEG C of above-mentioned steps cultivation temperature, 2000~3000LUX of intensity of illumination, light in root media
According to hour/day of time 6~12；Acclimatization and transplantses；Transplantation of Regenerated Plantlets will be taken root to garden mould+peat (2:1) in matrix, shade net
Shelter from heat or light, humidity 60-70% goes out garden in 15~30 days.This method needs gnotobasis, and production cost is high, and giant knotweed propagation technique is complicated.
The Chinese invention patent of Publication No. " CN104255502A " discloses a kind of giant knotweed tissue-culturing quick-propagation side
Method, this method comprises the following steps：（1）The selection and sterilizing of explant, choose the wild giant knotweed band axillary bud of healthy and strong, no disease and pests harm
Edible tender branch is as explant, and the processing that carried out disinfection to it；（2）The induction of adventitious bud, by step（1）In explant be cut into
Long 2-3cm stem-segment with single bud, is inoculated in inducing culture and carries out Fiber differentiation respectively, condition of culture：Temperature is 25 ± 2
DEG C, intensity of illumination 1500-2000lux, light application time 14-16h；（3）The steps such as the squamous subculture of adventitious bud.This method is mainly
Fast culture is carried out to giant knotweed tissue, it is impossible to carry out large-scale culture, germination percentage and survival rate are relatively low.
The content of the invention
High it is an object of the invention to overcome the deficiencies of the prior art and provide a kind of survival rate, cost is low, is adapted to extensive
The method of breeding giant knotweed seedling rapidly and efficiently.
A kind of method for rapidly and efficiently breeding giant knotweed seedling of the present invention, comprises the following steps：
A, press strip selection of time are in annual 5~June, and breeding and seedling environmental requirement is ventilated, irrigation and drainage convenient, and shading rate 70~
80%, control relative humidity 75~85%；
The life then of b, straight snips, the top stipes of robust growth no disease and pests harm, semi-lignified giant knotweed plant with terminal bud do cuttage cutting,
Cutting is dipped in the mud containing root-growing agent, insertion river sand matrix, pours permeable after cuttage immediately, sand moistening is kept, obtains band
The cuttage seeding of terminal bud；
C, the remaining giant knotweed plant secateurs trimming blade for cutting off the top stipes with terminal bud, leave and take 3~5 close to top
Leaf, to top otch sterilization, with film bundling, then the mound since plant base portion, claps consolidation in mound, and heap is to there is leaf
The place of piece is buried petiole with soil, 1.5~2 centimetres at the top of the height distance plant of mound, pours permeable after the completion of press strip immediately,
Mound moistening is kept, press strip plant is obtained；
D, treat the cuttage seeding with terminal bud and press strip plant leaf scar position adventitious root generation, now the cuttage seeding with terminal bud can be done
Seedling is transplanted, and press strip plant needs first all to dig mound and opened, and mound plane uses secateurs from 2~3 leaf scars of plant base portion after opening
Plant is cut in top, and the position of root of hair on the erect stern for isolating maternal plant is cut by the length of every 2~3 centimetres of section, is transplanted to
Grown in Nutrition Soil, produce giant knotweed seedling.
A kind of above-mentioned method for rapidly and efficiently breeding giant knotweed seedling, wherein the mud containing root-growing agent is by yellow mud:Root-growing agent
The aqueous solution is according to mass ratio 1:1~3 mixes, and is preferably according to mass ratio 1:2 mix, the root-growing agent be ABT3 or
ABT6。
ABT root-inducing powders are a kind of wide spectrum, efficient, compound plant growth regulator, and cost performance is high, root system can be promoted to send out
Educate, improve survival rate, increase anti-adversity ability, played an important role in cutting propagation.
Further, a kind of above-mentioned method for rapidly and efficiently breeding giant knotweed seedling, wherein ABT3 the or ABT6 aqueous solution
Concentration is 100~150mg/L.
A kind of above-mentioned method for rapidly and efficiently breeding giant knotweed seedling, wherein 8~10 centimetres of the cuttage cutting length, only stays terminal bud
And 2~3 young leaves around terminal bud, the blade of bottom all wipes out, and the leaf left and taken retains complete, without cutting.
A kind of above-mentioned method for rapidly and efficiently breeding giant knotweed seedling, the depth for inserting cutting in river sand matrix wherein in b step
Spend for 3~4 centimetres.
It is above-mentioned it is a kind of rapidly and efficiently breed giant knotweed seedling method, wherein in step c with Nutrition Soil since plant base portion heap
Soil.
A kind of above-mentioned method for rapidly and efficiently breeding giant knotweed seedling, wherein the Nutrition Soil is by yellow soil:Fertile soil:Peat
Soil:Vermiculite in mass ratio 2~4:2~4:0.5~1.5:0.5~1.5 mixes, or by yellow soil:Fertile soil:Peat
Soil:Perlite in mass ratio 2~4:2~4:0.5~1.5:0.5~1.5 mixes.Because giant knotweed prefer it is loose, fertile
The soil of slant acidity, and yellow soil is the cubsoil below soil layer, is the red soil of limestone development, the certain viscosity of tool, pH value 4~6；
Fertile soil, peat soil are slant acidity, loose, fertile soil.
Further, a kind of above-mentioned method for rapidly and efficiently breeding giant knotweed seedling, wherein the Nutrition Soil is by yellow soil:Humic
Soil:Peat soil:Vermiculite in mass ratio 3:3:l:L is mixed, or by yellow soil:Fertile soil:Peat soil:Perlite presses matter
Amount compares 3:3:l:L is mixed.
A kind of above-mentioned method for rapidly and efficiently breeding giant knotweed seedling, be to top otch sterilization wherein described in step c
Top otch is smeared with 0.5%wt~1wt% carbendazim, is wrapped up with breathable films.
Cuttage is combined by the propagation method of giant knotweed of the present invention with mound layering method, that is, has played the apical growth of giant knotweed
Advantage, meanwhile, the quick root of hair of semi-lignified stipes is made by easy heap indigenous method, plant resources is taken full advantage of, reached fast
The purpose of speed breeding.The propagation method of the present invention breeds giant knotweed, can keep maternal merit and root survival is high, top
Stipes cuttage survival rate is up to 97%, and no base of leaf section Rooting percent is up to 93%, root of hair stipes survival rate 100%.This method is easy to operate,
It is with low cost, with good market application foreground, for realizing that the large-scale breeding of giant knotweed is promoted with very important meaning
Justice.
Embodiment
Technical scheme is described in further detail with reference to specific embodiment, but protection scope of the present invention is not
It is confined to as described below.
Embodiment 1
The breeding of giant knotweed seedling：
A, press strip selection of time are in 5 annual~June, and breeding and seedling environmental requirement is ventilated, irrigation and drainage convenient, and shading rate 70~
80%, control relative humidity 75~85%；
B, with the life then of secateurs straight snips, the top stipes of robust growth no disease and pests harm, semi-lignified giant knotweed plant with terminal bud do skewer
Cutting is inserted, 8~10 centimetres of cutting length only stays 2~3 young leaves around terminal bud and terminal bud, and the blade of bottom is all wiped out, left and taken
Leaf retains complete, and without cutting, cutting is dipped into the mud containing root-growing agent, the mud containing root-growing agent be by yellow mud and 100~
The 150mg/L ABT3 aqueous solution is according to mass ratio 1:2 mix, 3~4 centimetres, the part with leaf scar in insertion river sand matrix
It is wholly embedded into sand, pours permeable after cuttage immediately, daily management requires that sand remains moistening；
C, the remaining giant knotweed plant secateurs trimming blade for cutting off the top stipes with terminal bud, leave and take 3~5 close to top
Leaf, smears top otch with 0.5wt% carbendazim, to top otch sterilization, is then wrapped up with breathable films, then with seeking
Soil mound since plant base portion is supported, Nutrition Soil is by yellow soil:Fertile soil:Peat soil:Vermiculite（Or perlite）In mass ratio
3:3:l:L is mixed, and consolidation is clapped in mound, and heap is buried petiole with soil to vaned place, the height distance of mound
1.5~2 centimetres at the top of plant, pour permeable after the completion of press strip immediately, daily management requires that mound remains moistening；
D, treat the cuttage seeding with terminal bud and press strip plant leaf scar position adventitious root generation, now the cuttage seeding with terminal bud can be done
Seedling is transplanted, and the plant of mound layering needs first all to dig mound and opened, and mound plane uses secateurs from plant base portion 2~3 after opening
Plant is cut above individual leaf scar, is cut into the position of root of hair on the erect stern for isolating maternal plant by the length of every 2~3 centimetres of section
It is transplanted in Nutrition Soil and grows, you can obtains giant knotweed high quality seedling.
Above-mentioned high quality seedling is transplanted in field test ground, experimental field preceding as corn, physical features is flat, fertility is uniform,
Loam, soil nutrient is as follows：PH value 7.53, full potassium, full nitrogen, full phosphorus, the content of organic matter be respectively 46.77g/kg, 0.72g/kg,
0.15g/kg, 94.40g/kg, available nitrogen, available phosphorus and quick-acting potassium content be respectively 154.40mg/kg, 56.27mg/kg,
184.35mg/kg.With RANDOMIZED BLOCK DESIGN, 3 repetitions, plot area 3.6m2（3m×1.2m）, seeding row spacing 40cm × 60cm,
Totally 6 row, often 4 plants of row plant 24 plants per cell altogether, cultivation and the same Production of Large Fields of field management.Finally, top stipes cutting survival
Rate is up to 95%, and no base of leaf section Rooting percent is up to 90%, root of hair stipes survival rate 100%.
Embodiment 2
The breeding of giant knotweed seedling：
A, press strip selection of time are in 5 annual~June, and breeding and seedling environmental requirement is ventilated, irrigation and drainage convenient, and shading rate 70~
80%, control relative humidity 75~85%；
B, with the life then of secateurs straight snips, the top stipes of robust growth no disease and pests harm, semi-lignified giant knotweed plant with terminal bud do skewer
Cutting is inserted, 8~10 centimetres of cutting length only stays 2~3 young leaves around terminal bud and terminal bud, and the blade of bottom is all wiped out, left and taken
Leaf retains complete, and without cutting, cutting is dipped into the mud containing root-growing agent, the mud containing root-growing agent be by yellow mud and 100~
The 100mg/L ABT6 aqueous solution is according to mass ratio 1:3 mix, 3~4 centimetres, the part with leaf scar in insertion river sand matrix
It is wholly embedded into sand, pours permeable after cuttage immediately, daily management requires that sand remains moistening；
C, the remaining giant knotweed plant secateurs trimming blade for cutting off the top stipes with terminal bud, leave and take 3~5 close to top
Leaf, smears top otch with 1wt% carbendazim, to top otch sterilization, is then wrapped up with breathable films, then use nutrition
Soil mound since plant base portion, Nutrition Soil is by yellow soil:Fertile soil:Peat soil:Perlite in mass ratio 2:4:l.5:l.5
Mix, consolidation is clapped in mound, heap is buried petiole with soil to vaned place, at the top of the height distance plant of mound
1.5~2 centimetres, pour permeable after the completion of press strip immediately, daily management requires that mound remains moistening；
D, treat the cuttage seeding with terminal bud and press strip plant leaf scar position adventitious root generation, now the cuttage seeding with terminal bud can be done
Seedling is transplanted, and the plant of mound layering needs first all to dig mound and opened, and mound plane uses secateurs from plant base portion 2~3 after opening
Plant is cut above individual leaf scar, is cut into the position of root of hair on the erect stern for isolating maternal plant by the length of every 2~3 centimetres of section
It is transplanted in Nutrition Soil and grows, you can obtains giant knotweed high quality seedling.
Above-mentioned high quality seedling is transplanted in field test ground, experimental field preceding as corn, physical features is flat, fertility is uniform,
Loam, soil nutrient is as follows：PH value 7.52, full potassium, full nitrogen, full phosphorus, the content of organic matter be respectively 46.75g/kg, 0.70g/kg,
0.16g/kg, 94.42g/kg, available nitrogen, available phosphorus and quick-acting potassium content be respectively 154.45mg/kg, 56.30mg/kg,
184.33mg/kg.With RANDOMIZED BLOCK DESIGN, 3 repetitions, plot area 3.6m2（3m×1.2m）, seeding row spacing 40cm × 60cm,
Totally 6 row, often 4 plants of row plant 24 plants per cell altogether, cultivation and the same Production of Large Fields of field management.Finally, top stipes cutting survival
Rate is up to 96%, and no base of leaf section Rooting percent is up to 92%, root of hair stipes survival rate 100%.
Embodiment 3
The breeding of giant knotweed seedling：
A, press strip selection of time are in 5 annual~June, and breeding and seedling environmental requirement is ventilated, irrigation and drainage convenient, and shading rate 70~
80%, control relative humidity 75~85%；
B, with the life then of secateurs straight snips, the top stipes of robust growth no disease and pests harm, semi-lignified giant knotweed plant with terminal bud do skewer
Cutting is inserted, 8~10 centimetres of cutting length only stays 2~3 young leaves around terminal bud and terminal bud, and the blade of bottom is all wiped out, left and taken
Leaf retains complete, and without cutting, cutting is dipped into the mud containing root-growing agent, the mud containing root-growing agent be by yellow mud and 100~
The 120mg/L ABT3 aqueous solution is according to mass ratio 1:1 mixes, 3~4 centimetres, the part with leaf scar in insertion river sand matrix
It is wholly embedded into sand, pours permeable after cuttage immediately, daily management requires that sand remains moistening；
C, the remaining giant knotweed plant secateurs trimming blade for cutting off the top stipes with terminal bud, leave and take 3~5 close to top
Leaf, smears top otch with 0.8wt% carbendazim, to top otch sterilization, is then wrapped up with breathable films, then with seeking
Soil mound since plant base portion is supported, Nutrition Soil is by yellow soil:Fertile soil:Peat soil:Vermiculite in mass ratio 3:2:l.5:0.5
Mix, consolidation is clapped in mound, heap is buried petiole with soil to vaned place, at the top of the height distance plant of mound
1.5~2 centimetres, pour permeable after the completion of press strip immediately, daily management requires that mound remains moistening；
D, treat the cuttage seeding with terminal bud and press strip plant leaf scar position adventitious root generation, now the cuttage seeding with terminal bud can be done
Seedling is transplanted, and the plant of mound layering needs first all to dig mound and opened, and mound plane uses secateurs from plant base portion 2~3 after opening
Plant is cut above individual leaf scar, is cut into the position of root of hair on the erect stern for isolating maternal plant by the length of every 2~3 centimetres of section
It is transplanted in Nutrition Soil and grows, you can obtains giant knotweed high quality seedling.
Above-mentioned high quality seedling is transplanted in field test ground, experimental field preceding as corn, physical features is flat, fertility is uniform,
Loam, soil nutrient is as follows：PH value 7.50, full potassium, full nitrogen, full phosphorus, the content of organic matter be respectively 46.76g/kg, 0.72g/kg,
0.15g/kg, 94.44g/kg, available nitrogen, available phosphorus and quick-acting potassium content be respectively 154.46mg/kg, 56.32mg/kg,
184.35mg/kg.With RANDOMIZED BLOCK DESIGN, 3 repetitions, plot area 3.6m2（3m×1.2m）, seeding row spacing 40cm × 60cm,
Totally 6 row, often 4 plants of row plant 24 plants per cell altogether, cultivation and the same Production of Large Fields of field management.Finally, top stipes cutting survival
Rate is up to 97%, and no base of leaf section Rooting percent is up to 93%, root of hair stipes survival rate 100%.
Described above is only the preferred embodiment of the present invention, it should be understood that the present invention is not limited to described herein
Form, is not to be taken as the exclusion to other embodiment, and available for various other combinations, modification and environment, and can be at this
In the text contemplated scope, it is modified by the technology or knowledge of above-mentioned teaching or association area.And those skilled in the art are entered
Capable change and change does not depart from the spirit and scope of the present invention, then all should appended claims of the present invention protection domain
It is interior.
Claims (9)
1. a kind of method for rapidly and efficiently breeding giant knotweed seedling, it is characterised in that comprise the following steps：
A, press strip selection of time are in annual 5~June, and breeding and seedling environmental requirement is ventilated, irrigation and drainage convenient, and shading rate 70~
80%, control relative humidity 75~85%；
The life then of b, straight snips, the top stipes of robust growth no disease and pests harm, semi-lignified giant knotweed plant with terminal bud do cuttage cutting,
Cutting is dipped in the mud containing root-growing agent, insertion river sand matrix, pours permeable after cuttage immediately, sand moistening is kept, obtains band
The cuttage seeding of terminal bud；
C, the remaining giant knotweed plant secateurs trimming blade for cutting off the top stipes with terminal bud, leave and take 3~5 close to top
Leaf, to top otch sterilization, with film bundling, then the mound since plant base portion, claps consolidation in mound, and heap is to there is leaf
The place of piece is buried petiole with soil, 1.5~2 centimetres at the top of the height distance plant of mound, pours permeable after the completion of press strip immediately,
Mound moistening is kept, press strip plant is obtained；
D, treat the cuttage seeding with terminal bud and press strip plant leaf scar position adventitious root generation, now the cuttage seeding with terminal bud can be done
Seedling is transplanted, and press strip plant needs first all to dig mound and opened, and mound plane uses secateurs from 2~3 leaf scars of plant base portion after opening
Plant is cut in top, and the position of root of hair on the erect stern for isolating maternal plant is cut by the length of every 2~3 centimetres of section, is transplanted to
Grown in Nutrition Soil, produce giant knotweed seedling.
2. a kind of method for rapidly and efficiently breeding giant knotweed seedling according to claim 1, it is characterised in that described to contain root-growing agent
Mud by yellow mud:The root-growing agent aqueous solution is according to mass ratio 1:1~3 mixes, and is preferably according to mass ratio 1:2 mixing and
Into the root-growing agent is ABT3 or ABT6.
3. it is according to claim 2 it is a kind of rapidly and efficiently breed giant knotweed seedling method, it is characterised in that the ABT3 or
The concentration of the ABT6 aqueous solution is 100~150mg/L.
4. a kind of method for rapidly and efficiently breeding giant knotweed seedling according to claim 1, it is characterised in that the cuttage cutting
It is long 8~10 centimetres, 2~3 young leaves around terminal bud and terminal bud are only stayed, the blade of bottom is all wiped out, the leaf left and taken retains complete,
Without cutting.
5. a kind of method for rapidly and efficiently breeding giant knotweed seedling according to claim 1, it is characterised in that will be inserted in b step
Depth in bar insertion river sand matrix is 3~4 centimetres.
6. a kind of method for rapidly and efficiently breeding giant knotweed seedling according to claim 1, it is characterised in that with battalion in step c
Support soil mound since plant base portion.
7. it is according to claim 6 it is a kind of rapidly and efficiently breed giant knotweed seedling method, it is characterised in that the Nutrition Soil by
Yellow soil:Fertile soil:Peat soil:Vermiculite in mass ratio 2~4:2~4:0.5~1.5:0.5~1.5 mixes, or by Huang
Cubsoil:Fertile soil:Peat soil:Perlite in mass ratio 2~4:2~4:0.5~1.5:0.5~1.5 mixes.
8. it is according to claim 7 it is a kind of rapidly and efficiently breed giant knotweed seedling method, it is characterised in that the Nutrition Soil by
Yellow soil:Fertile soil:Peat soil:Vermiculite in mass ratio 3:3:l:L is mixed, or by yellow soil:Fertile soil:Peat soil:
Perlite in mass ratio 3:3:l:L is mixed.
9. a kind of method for rapidly and efficiently breeding giant knotweed seedling according to claim 1, it is characterised in that described in step c
It is to smear top otch with 0.5wt%~1wt% carbendazim to top otch sterilization, is wrapped up with breathable films.
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| CN110800577A (en) * | 2019-12-10 | 2020-02-18 | 广西壮族自治区林业科学研究院 | Breeding method for rapid blooming of polygonum capitatum flowers |
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