CN103157103A - 功能性流感病毒样颗粒(VLPs) - Google Patents
功能性流感病毒样颗粒(VLPs) Download PDFInfo
- Publication number
- CN103157103A CN103157103A CN2013100841712A CN201310084171A CN103157103A CN 103157103 A CN103157103 A CN 103157103A CN 2013100841712 A CN2013100841712 A CN 2013100841712A CN 201310084171 A CN201310084171 A CN 201310084171A CN 103157103 A CN103157103 A CN 103157103A
- Authority
- CN
- China
- Prior art keywords
- influenza
- virus
- vaccine
- vlp
- influenza virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 206010022000 influenza Diseases 0.000 title claims abstract description 139
- 239000002245 particle Substances 0.000 title claims abstract description 40
- 241000712461 unidentified influenza virus Species 0.000 claims abstract description 104
- 210000004027 cell Anatomy 0.000 claims abstract description 59
- 229960005486 vaccine Drugs 0.000 claims abstract description 39
- 101710172711 Structural protein Proteins 0.000 claims abstract description 26
- 241000238631 Hexapoda Species 0.000 claims abstract description 20
- 101710154606 Hemagglutinin Proteins 0.000 claims description 92
- 101710093908 Outer capsid protein VP4 Proteins 0.000 claims description 92
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 claims description 92
- 101710176177 Protein A56 Proteins 0.000 claims description 92
- 239000000185 hemagglutinin Substances 0.000 claims description 92
- 102000005348 Neuraminidase Human genes 0.000 claims description 90
- 108010006232 Neuraminidase Proteins 0.000 claims description 90
- 241000701447 unidentified baculovirus Species 0.000 claims description 37
- 239000000203 mixture Substances 0.000 claims description 35
- 230000000694 effects Effects 0.000 claims description 15
- 238000001338 self-assembly Methods 0.000 claims description 13
- 230000006798 recombination Effects 0.000 claims description 11
- 238000005215 recombination Methods 0.000 claims description 11
- 241000124008 Mammalia Species 0.000 claims description 7
- 239000002671 adjuvant Substances 0.000 claims description 6
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 5
- 238000010276 construction Methods 0.000 claims description 4
- 239000003085 diluting agent Substances 0.000 claims description 4
- 229940031348 multivalent vaccine Drugs 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 126
- 102000004169 proteins and genes Human genes 0.000 abstract description 104
- 241000700605 Viruses Species 0.000 abstract description 44
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 19
- 108700010900 influenza virus proteins Proteins 0.000 abstract description 14
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 230000003472 neutralizing effect Effects 0.000 abstract description 7
- 229920002521 macromolecule Polymers 0.000 abstract description 6
- 108010087302 Viral Structural Proteins Proteins 0.000 abstract description 4
- 230000002265 prevention Effects 0.000 abstract description 3
- 241000712431 Influenza A virus Species 0.000 abstract description 2
- 210000000170 cell membrane Anatomy 0.000 abstract description 2
- 230000002163 immunogen Effects 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 abstract 2
- 108010008038 Synthetic Vaccines Proteins 0.000 abstract 1
- 230000001747 exhibiting effect Effects 0.000 abstract 1
- 229940124551 recombinant vaccine Drugs 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 68
- 238000000034 method Methods 0.000 description 42
- 206010064097 avian influenza Diseases 0.000 description 36
- 108020004414 DNA Proteins 0.000 description 34
- 239000013612 plasmid Substances 0.000 description 28
- 208000002979 Influenza in Birds Diseases 0.000 description 26
- 101150109178 M1 gene Proteins 0.000 description 26
- 229930006000 Sucrose Natural products 0.000 description 25
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 25
- 238000000746 purification Methods 0.000 description 25
- 229960004793 sucrose Drugs 0.000 description 25
- 239000008187 granular material Substances 0.000 description 20
- 230000003612 virological effect Effects 0.000 description 20
- 238000001262 western blot Methods 0.000 description 19
- 102000011931 Nucleoproteins Human genes 0.000 description 17
- 108010061100 Nucleoproteins Proteins 0.000 description 17
- 235000013681 dietary sucrose Nutrition 0.000 description 17
- 208000037797 influenza A Diseases 0.000 description 17
- 230000036039 immunity Effects 0.000 description 16
- 239000013598 vector Substances 0.000 description 16
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 13
- 229960003971 influenza vaccine Drugs 0.000 description 11
- 239000002773 nucleotide Substances 0.000 description 11
- 125000003729 nucleotide group Chemical group 0.000 description 11
- 238000012546 transfer Methods 0.000 description 11
- 238000004458 analytical method Methods 0.000 description 10
- 229940088679 drug related substance Drugs 0.000 description 10
- 230000005847 immunogenicity Effects 0.000 description 10
- 208000015181 infectious disease Diseases 0.000 description 10
- 210000002966 serum Anatomy 0.000 description 10
- 230000009385 viral infection Effects 0.000 description 10
- 239000008186 active pharmaceutical agent Substances 0.000 description 9
- 230000009849 deactivation Effects 0.000 description 9
- 238000013467 fragmentation Methods 0.000 description 9
- 238000006062 fragmentation reaction Methods 0.000 description 9
- 238000003752 polymerase chain reaction Methods 0.000 description 9
- 230000001681 protective effect Effects 0.000 description 9
- 239000002299 complementary DNA Substances 0.000 description 8
- 230000029087 digestion Effects 0.000 description 8
- 230000035931 haemagglutination Effects 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 8
- 239000005720 sucrose Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 7
- 241000699670 Mus sp. Species 0.000 description 7
- 241000283973 Oryctolagus cuniculus Species 0.000 description 7
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 7
- 238000001493 electron microscopy Methods 0.000 description 7
- 239000011148 porous material Substances 0.000 description 7
- 101710182846 Polyhedrin Proteins 0.000 description 6
- 108700005077 Viral Genes Proteins 0.000 description 6
- 230000000890 antigenic effect Effects 0.000 description 6
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 238000010839 reverse transcription Methods 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 description 6
- 238000010186 staining Methods 0.000 description 6
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 description 5
- 101150046652 M2 gene Proteins 0.000 description 5
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 239000012228 culture supernatant Substances 0.000 description 5
- 238000010790 dilution Methods 0.000 description 5
- 239000012895 dilution Substances 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 239000012634 fragment Substances 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 102000057593 human F8 Human genes 0.000 description 5
- 150000002632 lipids Chemical class 0.000 description 5
- 229940047431 recombinate Drugs 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 210000002845 virion Anatomy 0.000 description 5
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 4
- 101710132601 Capsid protein Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 108060003951 Immunoglobulin Proteins 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 101150118742 NP gene Proteins 0.000 description 4
- 108010006886 Vitrogen Proteins 0.000 description 4
- 239000000427 antigen Substances 0.000 description 4
- 210000003743 erythrocyte Anatomy 0.000 description 4
- 239000013604 expression vector Substances 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 238000010376 human cloning Methods 0.000 description 4
- 230000003053 immunization Effects 0.000 description 4
- 238000002649 immunization Methods 0.000 description 4
- 102000018358 immunoglobulin Human genes 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 238000001890 transfection Methods 0.000 description 4
- 238000005199 ultracentrifugation Methods 0.000 description 4
- 229960004854 viral vaccine Drugs 0.000 description 4
- 238000011725 BALB/c mouse Methods 0.000 description 3
- 241000283707 Capra Species 0.000 description 3
- 241000700199 Cavia porcellus Species 0.000 description 3
- 229920002307 Dextran Polymers 0.000 description 3
- 101150039660 HA gene Proteins 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 102000003505 Myosin Human genes 0.000 description 3
- 108060008487 Myosin Proteins 0.000 description 3
- 101150080862 NA gene Proteins 0.000 description 3
- 101710144128 Non-structural protein 2 Proteins 0.000 description 3
- 101710199667 Nuclear export protein Proteins 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 108700020497 Nucleopolyhedrovirus polyhedrin Proteins 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 3
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 3
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 3
- 108050000930 Polymerase acidic proteins Proteins 0.000 description 3
- 101710102873 Polymerase basic protein 2 Proteins 0.000 description 3
- 101710085035 RNA-directed RNA polymerase catalytic subunit Proteins 0.000 description 3
- 108020000999 Viral RNA Proteins 0.000 description 3
- 208000036142 Viral infection Diseases 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000010241 blood sampling Methods 0.000 description 3
- 239000008366 buffered solution Substances 0.000 description 3
- 238000005352 clarification Methods 0.000 description 3
- 230000007850 degeneration Effects 0.000 description 3
- 238000005227 gel permeation chromatography Methods 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 229940125575 vaccine candidate Drugs 0.000 description 3
- 241000201370 Autographa californica nucleopolyhedrovirus Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 208000003322 Coinfection Diseases 0.000 description 2
- 102000012410 DNA Ligases Human genes 0.000 description 2
- 108010061982 DNA Ligases Proteins 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 229920001503 Glucan Polymers 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- 108090000288 Glycoproteins Proteins 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 2
- 241000700721 Hepatitis B virus Species 0.000 description 2
- 241000371980 Influenza B virus (B/Shanghai/361/2002) Species 0.000 description 2
- 229940124873 Influenza virus vaccine Drugs 0.000 description 2
- 241001500351 Influenzavirus A Species 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 108010076504 Protein Sorting Signals Proteins 0.000 description 2
- 108010001267 Protein Subunits Proteins 0.000 description 2
- 102000002067 Protein Subunits Human genes 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 241000256251 Spodoptera frugiperda Species 0.000 description 2
- 229930003779 Vitamin B12 Natural products 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- AQLMHYSWFMLWBS-UHFFFAOYSA-N arsenite(1-) Chemical compound O[As](O)[O-] AQLMHYSWFMLWBS-UHFFFAOYSA-N 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000006143 cell culture medium Substances 0.000 description 2
- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 description 2
- 238000004440 column chromatography Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000013613 expression plasmid Substances 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 108020001507 fusion proteins Proteins 0.000 description 2
- 102000037865 fusion proteins Human genes 0.000 description 2
- 230000003067 hemagglutinative effect Effects 0.000 description 2
- 230000008105 immune reaction Effects 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- TWNIBLMWSKIRAT-VFUOTHLCSA-N levoglucosan Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@H]2CO[C@@H]1O2 TWNIBLMWSKIRAT-VFUOTHLCSA-N 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 229940127554 medical product Drugs 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 230000008488 polyadenylation Effects 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 239000013615 primer Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000003757 reverse transcription PCR Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000009182 swimming Effects 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 238000002255 vaccination Methods 0.000 description 2
- 239000011715 vitamin B12 Substances 0.000 description 2
- 235000019163 vitamin B12 Nutrition 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 description 1
- QRXMUCSWCMTJGU-UHFFFAOYSA-N 5-bromo-4-chloro-3-indolyl phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP(O)(=O)O)=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-N 0.000 description 1
- 101100272788 Arabidopsis thaliana BSL3 gene Proteins 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 101150011571 BSL2 gene Proteins 0.000 description 1
- 241000701412 Baculoviridae Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000324343 Causa Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 238000011238 DNA vaccination Methods 0.000 description 1
- 241000450599 DNA viruses Species 0.000 description 1
- 101710118188 DNA-binding protein HU-alpha Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 101710121417 Envelope glycoprotein Proteins 0.000 description 1
- 241000283073 Equus caballus Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 229940124895 FluMist Drugs 0.000 description 1
- 229940124893 Fluvirin Drugs 0.000 description 1
- 229940124894 Fluzone Drugs 0.000 description 1
- 229930186217 Glycolipid Natural products 0.000 description 1
- 101150111468 H3 gene Proteins 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 239000007987 MES buffer Substances 0.000 description 1
- 241000701076 Macacine alphaherpesvirus 1 Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 241000712464 Orthomyxoviridae Species 0.000 description 1
- 206010067268 Post procedural infection Diseases 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 241000711931 Rhabdoviridae Species 0.000 description 1
- 102000004389 Ribonucleoproteins Human genes 0.000 description 1
- 108010081734 Ribonucleoproteins Proteins 0.000 description 1
- 102100021696 Syncytin-1 Human genes 0.000 description 1
- 241000710914 Totivirus Species 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 108010093857 Viral Hemagglutinins Proteins 0.000 description 1
- 108010067390 Viral Proteins Proteins 0.000 description 1
- 230000010530 Virus Neutralization Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- VEZXCJBBBCKRPI-UHFFFAOYSA-N beta-propiolactone Chemical compound O=C1CCO1 VEZXCJBBBCKRPI-UHFFFAOYSA-N 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 210000000234 capsid Anatomy 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- -1 cation lipid Chemical class 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 229940028617 conventional vaccine Drugs 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000000635 electron micrograph Methods 0.000 description 1
- 210000001163 endosome Anatomy 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 102000054766 genetic haplotypes Human genes 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000001524 infective effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 210000001739 intranuclear inclusion body Anatomy 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 210000003071 memory t lymphocyte Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229940126619 mouse monoclonal antibody Drugs 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 238000011587 new zealand white rabbit Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 108091027963 non-coding RNA Proteins 0.000 description 1
- 102000042567 non-coding RNA Human genes 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000020030 perry Nutrition 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 229940023143 protein vaccine Drugs 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 230000010837 receptor-mediated endocytosis Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000009666 routine test Methods 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 125000005629 sialic acid group Chemical group 0.000 description 1
- 108010061514 sialic acid receptor Proteins 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 229940031626 subunit vaccine Drugs 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 229940031418 trivalent vaccine Drugs 0.000 description 1
- 230000029812 viral genome replication Effects 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- 239000000277 virosome Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- A61K39/145—Orthomyxoviridae, e.g. influenza virus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C07K14/08—RNA viruses
- C07K14/11—Orthomyxoviridae, e.g. influenza virus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5258—Virus-like particles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/57—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2
- A61K2039/575—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2 humoral response
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/70—Multivalent vaccine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16023—Virus like particles [VLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16123—Virus like particles [VLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/10—Detection of antigens from microorganism in sample from host
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Virology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Biomedical Technology (AREA)
- Pulmonology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- General Engineering & Computer Science (AREA)
- Communicable Diseases (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Urology & Nephrology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physics & Mathematics (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/617,569 | 2003-07-11 | ||
| US10/617,569 US8592197B2 (en) | 2003-07-11 | 2003-07-11 | Functional influenza virus-like particles (VLPs) |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2004800261523A Division CN1849134A (zh) | 2003-07-11 | 2004-07-09 | 功能性流感病毒样颗粒(VLPs) |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103157103A true CN103157103A (zh) | 2013-06-19 |
Family
ID=33565000
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013100841712A Pending CN103157103A (zh) | 2003-07-11 | 2004-07-09 | 功能性流感病毒样颗粒(VLPs) |
| CNA2004800261523A Pending CN1849134A (zh) | 2003-07-11 | 2004-07-09 | 功能性流感病毒样颗粒(VLPs) |
| CN201710658288.5A Pending CN107513102A (zh) | 2003-07-11 | 2004-07-09 | 功能性流感病毒样颗粒(VLPs) |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2004800261523A Pending CN1849134A (zh) | 2003-07-11 | 2004-07-09 | 功能性流感病毒样颗粒(VLPs) |
| CN201710658288.5A Pending CN107513102A (zh) | 2003-07-11 | 2004-07-09 | 功能性流感病毒样颗粒(VLPs) |
Country Status (19)
| Country | Link |
|---|---|
| US (5) | US8592197B2 (enExample) |
| EP (2) | EP2343084B1 (enExample) |
| JP (5) | JP2007529997A (enExample) |
| KR (1) | KR101153929B1 (enExample) |
| CN (3) | CN103157103A (enExample) |
| AT (1) | ATE538810T1 (enExample) |
| AU (1) | AU2004268510B2 (enExample) |
| BR (1) | BRPI0412519A (enExample) |
| CA (1) | CA2532335C (enExample) |
| DK (2) | DK1644037T3 (enExample) |
| ES (2) | ES2422580T3 (enExample) |
| IL (1) | IL173071A (enExample) |
| MX (1) | MXPA06000469A (enExample) |
| NZ (1) | NZ544580A (enExample) |
| PL (2) | PL1644037T3 (enExample) |
| PT (2) | PT2343084E (enExample) |
| RU (1) | RU2369405C2 (enExample) |
| SG (2) | SG165378A1 (enExample) |
| WO (1) | WO2005020889A2 (enExample) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107488216A (zh) * | 2017-09-06 | 2017-12-19 | 中国人民解放军军事医学科学院微生物流行病研究所 | 一种流感病毒通用疫苗及其制备方法与应用 |
| CN110240634A (zh) * | 2018-03-08 | 2019-09-17 | 普莱柯生物工程股份有限公司 | 一种禽流感病毒样颗粒疫苗、及其制备方法和应用 |
| CN113398259A (zh) * | 2021-04-30 | 2021-09-17 | 吉林大学 | 一种h9n2亚型禽流感新型标记疫苗的制备方法及其应用 |
| CN113461786A (zh) * | 2020-03-30 | 2021-10-01 | 普莱柯生物工程股份有限公司 | 一种禽流感病毒样颗粒疫苗、及其制备方法和应用 |
Families Citing this family (158)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2217967B1 (es) * | 2003-03-31 | 2006-01-01 | Consejo Sup. Investig. Cientificas | Procedimiento de produccion de particulas virales vacias (vlps) del virus inductor de la bursitis infecciosa (ibdv), composiciones necesarias para su puesta a punto y su uso en la elaboracion de vacunas frente al ibdv. |
| US8206950B2 (en) * | 2003-06-09 | 2012-06-26 | Animal Technology Institute Taiwan | Fusion antigen used as vaccine and method of making them |
| US20160000902A1 (en) * | 2003-07-11 | 2016-01-07 | Novavax, Inc. | Combination vaccine for respiratory syncytial virus and influenza |
| US8506967B2 (en) | 2003-07-11 | 2013-08-13 | Novavax, Inc. | Functional influenza virus like particles (VLPs) |
| US8592197B2 (en) * | 2003-07-11 | 2013-11-26 | Novavax, Inc. | Functional influenza virus-like particles (VLPs) |
| US8551756B2 (en) | 2003-07-11 | 2013-10-08 | Novavax, Inc. | Avian influenza chimeric VLPS |
| US8080255B2 (en) | 2003-07-11 | 2011-12-20 | Novavax Inc. | Functional influenza virus like particles (VLPs) |
| US8992939B2 (en) | 2003-07-11 | 2015-03-31 | Novavax, Inc. | Highly efficient influenza matrix (M1) proteins |
| ES2307346B1 (es) * | 2004-01-21 | 2009-11-13 | Consejo Sup. Investig. Cientificas | Capsidas vacias (vlps(-vp4)) del virus causante de la enfermedad de la bursitis infecciosa (ibdv), su procedimiento de obtencion y aplicaciones. |
| ES2307345B1 (es) * | 2004-01-21 | 2009-11-13 | Consejo Sup. Investig. Cientificas | Capsidas vacias quimericas del virus causante de la enfermedad de la bursitis infecciosa (ibdv), su procedimiento de obtencion y aplicaciones. |
| GB0716992D0 (en) | 2007-08-31 | 2007-10-10 | Immune Targeting Systems Its L | Influenza antigen delivery vectors and constructs |
| GB0408164D0 (en) | 2004-04-13 | 2004-05-19 | Immune Targeting Systems Ltd | Antigen delivery vectors and constructs |
| DE602005022728D1 (de) * | 2004-08-19 | 2010-09-16 | Univ Cardiff | Präparation antigenpräsentierender menschlicher gamma delta t-zellen und verwendung in der immuntherapie |
| US20090208517A1 (en) * | 2004-08-19 | 2009-08-20 | Bernhard Moser | Preparation Of Antigen-Presenting Human Gamma-Delta T Cells And Use In Immunotherapy |
| CA2597928A1 (en) * | 2005-02-16 | 2006-08-24 | Lentigen Corporation | Lentiviral vectors and their use |
| ES2570758T3 (es) | 2005-04-21 | 2016-05-20 | Univ Florida | Materiales y métodos para el control de enfermedades respiratorias en cánidos |
| ES2310062B1 (es) * | 2005-07-15 | 2009-11-13 | Bionostra, S.L. | Particulas pseudovirales vacias quimericas derivadas del virus causante de la enfermedad de la bursitis infecciosa (ibdv), procedimiento de obtencion y aplicaciones. |
| US8338173B2 (en) * | 2005-08-11 | 2012-12-25 | University College Cardiff Consultants Limited | Preparation of antigen-presenting human γδ T cells and use in immunotherapy |
| AU2006304640B2 (en) * | 2005-10-18 | 2012-10-11 | Novavax, Inc. | Functional influenza virus like particles (VLPs) |
| KR101597534B1 (ko) | 2005-10-19 | 2016-02-26 | 유니버시티 오브 플로리다 리서치 파운데이션, 인크. | 개과 동물의 호흡기 질병 조절을 위한 물질들 및 방법들 |
| WO2008048269A2 (en) * | 2005-10-20 | 2008-04-24 | Combimatrix Corporation | Microarray for pathogen identification |
| GB2432419A (en) * | 2005-11-16 | 2007-05-23 | Agency Science Tech & Res | Influenza A virus detection method |
| FI20051255A0 (fi) * | 2005-12-05 | 2005-12-05 | Pekka Untamo Heino | Seuraavaa influenssa A-pandemiaa vastaan soveltuva rokote |
| US8232058B2 (en) * | 2006-01-20 | 2012-07-31 | Lawrence Livermore National Security, Llc | Multiplex detection of respiratory pathogens |
| JP5198290B2 (ja) * | 2006-02-02 | 2013-05-15 | グローブイミューン,インコーポレイテッド | 免疫反応を誘発する酵母ベースワクチン |
| GB0613977D0 (en) | 2006-02-07 | 2006-08-23 | Peptcell Ltd | Peptide sequences and compositions |
| US20070224205A1 (en) * | 2006-03-07 | 2007-09-27 | Powell Thomas J | Compositions that include hemagglutinin, methods of making and methods of use thereof |
| WO2007126788A2 (en) * | 2006-03-27 | 2007-11-08 | Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Methods and compositions for the treatment and prevention of viral infection |
| US20070243587A1 (en) * | 2006-04-14 | 2007-10-18 | Healthbanks Biotech Co., Ltd. | Using a reverse genetic engineering platform to produce protein vaccines and protein vaccine of avian influenza virus |
| WO2007130327A2 (en) | 2006-05-01 | 2007-11-15 | Technovax, Inc. | Influenza virus-like particle (vlp) compositions |
| US9101578B2 (en) * | 2006-05-01 | 2015-08-11 | Technovax, Inc. | Polyvalent influenza virus-like particle (VLP) compositions |
| US20100143393A1 (en) * | 2006-05-11 | 2010-06-10 | Gale Smith | Novel influenza m2 vaccines |
| KR100702034B1 (ko) * | 2006-05-12 | 2007-03-30 | 삼성전자주식회사 | 반도체 장치, 이 장치의 전원 노이즈 감소 방법 및 인쇄회로 기판 |
| US20100184832A1 (en) | 2006-07-14 | 2010-07-22 | Sanofi Pasteur Biologics Co. | Construction of Recombinant Virus Vaccines by Direct Transposon-Mediated Insertion of Foreign Immunologic Determinants into Vector Virus Proteins |
| AU2007345768B2 (en) | 2006-07-27 | 2013-08-01 | Ligocyte Pharmaceuticals, Inc. | Chimeric influenza virus-like particles |
| EP2044224A4 (en) * | 2006-07-27 | 2011-04-13 | Ligocyte Pharmaceuticals Inc | CHIMERIC VIRUS TYPE PARTICLES |
| US8080645B2 (en) * | 2007-10-01 | 2011-12-20 | Longhorn Vaccines & Diagnostics Llc | Biological specimen collection/transport compositions and methods |
| US8652782B2 (en) | 2006-09-12 | 2014-02-18 | Longhorn Vaccines & Diagnostics, Llc | Compositions and methods for detecting, identifying and quantitating mycobacterial-specific nucleic acids |
| US20090098527A1 (en) * | 2006-09-12 | 2009-04-16 | Fischer Gerald W | Biological organism identification product and methods |
| US9481912B2 (en) | 2006-09-12 | 2016-11-01 | Longhorn Vaccines And Diagnostics, Llc | Compositions and methods for detecting and identifying nucleic acid sequences in biological samples |
| US8097419B2 (en) * | 2006-09-12 | 2012-01-17 | Longhorn Vaccines & Diagnostics Llc | Compositions and method for rapid, real-time detection of influenza A virus (H1N1) swine 2009 |
| US20100239605A1 (en) * | 2006-09-29 | 2010-09-23 | Sanofi Pasteur Biologics Co. | Recombinant Rhinovirus Vectors |
| SG10202012986SA (en) * | 2006-09-29 | 2021-01-28 | Ligocyte Pharmaceuticals Inc | Norovirus vaccine formulations |
| WO2008058396A1 (en) * | 2006-11-15 | 2008-05-22 | Folia Biotech Inc. | Papaya mosaic virus-based vaccines for influenza |
| US8877206B2 (en) | 2007-03-22 | 2014-11-04 | Pds Biotechnology Corporation | Stimulation of an immune response by cationic lipids |
| CA2615372A1 (en) | 2007-07-13 | 2009-01-13 | Marc-Andre D'aoust | Influenza virus-like particles (vlps) comprising hemagglutinin |
| CA2693899A1 (en) * | 2007-07-19 | 2009-01-22 | Novavax, Inc. | Chimeric varicella zoster virus-virus like particles |
| US11041215B2 (en) | 2007-08-24 | 2021-06-22 | Longhorn Vaccines And Diagnostics, Llc | PCR ready compositions and methods for detecting and identifying nucleic acid sequences |
| US9683256B2 (en) | 2007-10-01 | 2017-06-20 | Longhorn Vaccines And Diagnostics, Llc | Biological specimen collection and transport system |
| EP2772267B1 (en) | 2007-08-27 | 2016-04-27 | Longhorn Vaccines and Diagnostics, LLC | Immunogenic compositions and methods |
| US10004799B2 (en) | 2007-08-27 | 2018-06-26 | Longhorn Vaccines And Diagnostics, Llc | Composite antigenic sequences and vaccines |
| CN101918028B (zh) | 2007-09-18 | 2015-01-07 | 莱戈赛特医药股份有限公司 | 赋予针对诺如病毒的保护性免疫应答的疫苗 |
| US20100266636A1 (en) | 2007-09-18 | 2010-10-21 | Ligocyte Pharmaceuticals, Inc. | Method of conferring a protective immune response to norovirus |
| US11041216B2 (en) | 2007-10-01 | 2021-06-22 | Longhorn Vaccines And Diagnostics, Llc | Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples |
| WO2009085355A2 (en) * | 2007-10-01 | 2009-07-09 | Longhorn Vaccines & Diagnostics Llc | Biological specimen collection and transport system and methods of use |
| EP2238253B1 (en) * | 2007-11-27 | 2012-09-12 | Medicago Inc. | Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin |
| KR101956910B1 (ko) * | 2008-01-21 | 2019-03-12 | 메디카고 인코포레이티드 | 헤마글루티닌을 발현하는 트랜스제닉 식물에서 생산된 재조합 인플루엔자 바이러스-유사 입자(VLPs) |
| WO2009102229A1 (ru) * | 2008-02-15 | 2009-08-20 | A.N. Belozersky Institute Of Physico-Chemical Biology, Moscow State University | Липид-протеиновый рафтовый комплекс из оболочки вируса гриппа а для противогриппозных вакцин и способ его выделения, способ получения вирусоподобных частиц из таких комплексов |
| BRPI0910464B1 (pt) | 2008-04-17 | 2021-08-10 | Pds Biotechnology Corporation | Uso de uso de um lipídio catiônico quiral consistindo de r-dotap a preparação de composição farmacêutica |
| JP2011519828A (ja) | 2008-04-18 | 2011-07-14 | バクシネート コーポレーション | フラジェリンの欠失変異体と使用方法 |
| US8450056B2 (en) * | 2008-05-02 | 2013-05-28 | University Of Rochester | Arrayed imaging reflectometry (AIR) sensor chip comprising virus-like particles suitable for the detection of antiviral immune responses |
| US8486619B2 (en) | 2008-05-02 | 2013-07-16 | University Of Rochester | Arrayed imaging reflectometry (air) sensor chip comprising influenza hemagglutinin (HA) polypeptides suitable for the detection of antiviral immune responses |
| CN101293099B (zh) * | 2008-06-04 | 2010-12-08 | 厦门大学 | 一种抗多种亚型禽流感病毒的多肽疫苗及其制备方法 |
| CA2730171C (en) | 2008-07-08 | 2016-10-11 | Medicago Inc. | Soluble recombinant influenza antigens |
| ES2593277T3 (es) * | 2008-07-18 | 2016-12-07 | Medicago Inc. | Nuevo epítopo de inmunización contra el virus de la influenza |
| CA2742288A1 (en) * | 2008-07-30 | 2010-02-04 | Folia Biotech Inc. | Multivalent vaccines based on papaya mosaic virus and uses thereof |
| IT1394782B1 (it) | 2009-03-03 | 2012-07-13 | Alenia Aeronautica Spa | Procedimento di predizione di collisioni tra un velivolo e un aeromobile |
| JP2012521786A (ja) | 2009-03-30 | 2012-09-20 | モウント シナイ スクール オフ メディシネ | インフルエンザウイルスワクチン及びその使用 |
| AU2010243489A1 (en) * | 2009-05-01 | 2011-12-22 | Redbiotec Ag | Recombinant virus-like particles encoded by multi-gene vector |
| US8673314B2 (en) * | 2009-05-26 | 2014-03-18 | Mount Sinai School Of Medicine | Monoclonal antibodies against influenza virus generated by cyclical administration and uses thereof |
| NZ597401A (en) | 2009-06-24 | 2013-09-27 | Medicago Inc | Chimeric influenza virus-like particles comprising hemagglutinin |
| CA2766899C (en) | 2009-07-02 | 2021-05-04 | Massachusetts Institute Of Technology | Compositions and methods for diagnosing and/or treating influenza infection |
| DK2480560T3 (en) | 2009-09-22 | 2018-04-16 | Medicago Inc | PROCEDURE FOR THE PREPARATION OF PLANT DERIVATIVE PROTEINS |
| US20110097358A1 (en) * | 2009-10-12 | 2011-04-28 | Techno Vax, Inc. | RESPIRATORY SYNCYTIAL VIRUS (RSV) VIRUS-LIKE PARTICLES (VLPs) |
| WO2011056899A2 (en) | 2009-11-03 | 2011-05-12 | Ligocyte Pharmaceuticals, Inc. | Chimeric rsv-f polypeptide and lentivirus or alpha-retrovirus gag-based vlps |
| JP2013520172A (ja) | 2010-02-18 | 2013-06-06 | モウント シナイ スクール オフ メディシネ | インフルエンザウイルス疾患の予防及び治療で用いるためのワクチン |
| US9352031B2 (en) | 2010-02-18 | 2016-05-31 | Technovax, Inc. | Universal virus-like particle (VLP) influenza vaccines |
| NZ602217A (en) | 2010-03-11 | 2014-08-29 | Immune Design Corp | Vaccines for influenza |
| US10130697B2 (en) | 2010-03-23 | 2018-11-20 | Wisconsin Alumni Research Foundation (Warf) | Vaccines comprising mutant attenuated influenza viruses |
| AU2011235220B2 (en) | 2010-03-30 | 2016-03-10 | Mount Sinai School Of Medicine | Influenza virus vaccines and uses thereof |
| US8822663B2 (en) | 2010-08-06 | 2014-09-02 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
| CN102373184A (zh) * | 2010-08-09 | 2012-03-14 | 中山大学 | 禽流感和传染性支气管炎混合病毒样颗粒、制备方法和应用 |
| CN102373182A (zh) * | 2010-08-09 | 2012-03-14 | 中山大学 | 禽流感和传染性囊病混合病毒样颗粒、制备方法和应用 |
| CA2811023A1 (en) | 2010-09-21 | 2012-03-29 | Massachusetts Institute Of Technology | Influenza treatment and/or characterization; human-adapted ha polypeptides; vaccines |
| CA2821992A1 (en) | 2010-10-01 | 2012-04-05 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
| BR112013007946B1 (pt) | 2010-10-04 | 2022-07-12 | Massachusetts Institute Of Technology | Composições farmacêuticas e imunogênicas compreendendo polipeptídeos de hemaglutinina |
| KR101847908B1 (ko) * | 2010-11-05 | 2018-04-11 | 노바백스, 인코포레이티드 | 광견병 당단백질 바이러스-유사 입자(VLPs) |
| EP2654787A1 (en) * | 2010-12-22 | 2013-10-30 | Novavax, Inc. | Modified influenza hemagglutinin proteins and uses thereof |
| TWI526539B (zh) * | 2010-12-22 | 2016-03-21 | 苜蓿股份有限公司 | 植物中生產類病毒顆粒(vlp)的方法及以該方法生產之vlp |
| TWI620816B (zh) | 2011-03-23 | 2018-04-11 | 苜蓿股份有限公司 | 植物衍生蛋白回收方法 |
| DE12722942T1 (de) | 2011-03-31 | 2021-09-30 | Modernatx, Inc. | Freisetzung und formulierung von manipulierten nukleinsäuren |
| KR20140047069A (ko) * | 2011-06-20 | 2014-04-21 | 유니버시티 오브 피츠버그 - 오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션 | 계산에 최적화된 광범위 반응을 나타내는 h1n1 인플루엔자를 위한 항원 |
| MX356586B (es) | 2011-07-11 | 2018-06-05 | Takeda Vaccines Inc | Formulaciones parenterales de vacunas contra los norovirus. |
| CN103945863A (zh) | 2011-08-01 | 2014-07-23 | 爱默蕾大学 | 包含配体的vlp及其相关方法 |
| WO2013019800A1 (en) | 2011-08-01 | 2013-02-07 | Emory University | Vlps containing ligands and methods related thereto |
| EP2747778B1 (en) | 2011-08-26 | 2017-12-06 | Wisconsin Alumni Research Foundation | Influenza viruses with mutant pb2 gene segment as live attenuated vaccines |
| US9464124B2 (en) | 2011-09-12 | 2016-10-11 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
| ES2953393T3 (es) | 2011-09-20 | 2023-11-10 | Icahn School Med Mount Sinai | Vacunas contra el virus de la influenza y sus usos |
| HRP20220250T1 (hr) | 2011-10-03 | 2022-04-29 | Modernatx, Inc. | Modificirani nukleozidi, nukleotidi i nukleinske kiseline, te njihove uporabe |
| SI2791160T1 (sl) | 2011-12-16 | 2022-07-29 | Modernatx, Inc. | Sestave modificirane MRNA |
| CA2863083C (en) | 2012-01-26 | 2023-09-19 | Longhorn Vaccines And Diagnostics, Llc | Composite antigenic sequences and vaccines |
| RU2653756C2 (ru) | 2012-02-07 | 2018-05-14 | Юниверсити Оф Питтсбург - Оф Зе Коммонвэлс Систем Оф Хайе Эдьюкейшн | Оптимизированные с помощью компьютера антигены с широким спектром реактивности для вирусов гриппа h3n2 |
| JP6175452B2 (ja) | 2012-02-13 | 2017-08-02 | ユニバーシティ オブ ピッツバーグ − オブ ザ コモンウェルス システム オブ ハイヤー エデュケイション | ヒトおよびトリh5n1インフルエンザのための、計算で最適化した反応性の広い抗原 |
| RU2485973C1 (ru) * | 2012-02-28 | 2013-06-27 | Общество с ограниченной ответственностью "НТфарма" | Рекомбинантная трехвалентная вакцина от гриппа человека |
| WO2013142329A1 (en) | 2012-03-22 | 2013-09-26 | Fraunhofer Usa, Inc. | Virus -like particles comprising a matrix protein from a plant enveloped virus and uses thereof |
| BR112014023900A2 (pt) | 2012-03-30 | 2020-11-24 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Polipeptídeo de hemaglutimina de influenza recombinante, molécula de ácido nucleicoisolada, vetor, célula isolada, partícula semelhante ao vírus da influenza, proteína de fusão, composição, e uso |
| US9572897B2 (en) | 2012-04-02 | 2017-02-21 | Modernatx, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
| AU2013243952A1 (en) | 2012-04-02 | 2014-10-30 | Modernatx, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
| US10501512B2 (en) | 2012-04-02 | 2019-12-10 | Modernatx, Inc. | Modified polynucleotides |
| US9283287B2 (en) | 2012-04-02 | 2016-03-15 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of nuclear proteins |
| WO2013177294A1 (en) * | 2012-05-22 | 2013-11-28 | Cornell University | Reagents and methods for identifying anti-hiv compounds |
| RU2649365C2 (ru) | 2012-06-15 | 2018-04-02 | ПиДиЭс БАЙОТЕКНОЛОДЖИ КОРПОРЭЙШН | Вакцинные композиции с катионными липидами и способы применения |
| US8932598B2 (en) | 2012-08-28 | 2015-01-13 | Vaxinnate Corporation | Fusion proteins and methods of use |
| CN105101991A (zh) | 2012-09-21 | 2015-11-25 | Pds生物科技公司 | 改进的疫苗组合物和使用方法 |
| KR101484588B1 (ko) | 2012-10-24 | 2015-01-20 | 대한민국 | 개 인플루엔자 vlp 백신의 제조방법 |
| SMT202200337T1 (it) | 2012-11-26 | 2022-09-14 | Modernatx Inc | Rna modificato al livello del terminale |
| AU2013352179B2 (en) | 2012-11-27 | 2017-12-21 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Computationally optimized broadly reactive antigens for H1N1 influenza |
| EP2934581B1 (en) | 2012-12-18 | 2022-09-14 | Icahn School of Medicine at Mount Sinai | Influenza virus vaccines and uses thereof |
| EP2971010B1 (en) | 2013-03-14 | 2020-06-10 | ModernaTX, Inc. | Formulation and delivery of modified nucleoside, nucleotide, and nucleic acid compositions |
| US9908930B2 (en) | 2013-03-14 | 2018-03-06 | Icahn School Of Medicine At Mount Sinai | Antibodies against influenza virus hemagglutinin and uses thereof |
| US8980864B2 (en) | 2013-03-15 | 2015-03-17 | Moderna Therapeutics, Inc. | Compositions and methods of altering cholesterol levels |
| IL318076A (en) | 2013-08-13 | 2025-02-01 | Univ Northwestern | Peptide-conjugated particles |
| CN103467581B (zh) * | 2013-08-26 | 2016-01-20 | 中国科学院微生物研究所 | 流感病毒m1蛋白及其编码基因和应用 |
| EP3041938A1 (en) | 2013-09-03 | 2016-07-13 | Moderna Therapeutics, Inc. | Circular polynucleotides |
| AU2014315287A1 (en) | 2013-09-03 | 2015-03-12 | Moderna Therapeutics, Inc. | Chimeric polynucleotides |
| US10023626B2 (en) | 2013-09-30 | 2018-07-17 | Modernatx, Inc. | Polynucleotides encoding immune modulating polypeptides |
| US10323076B2 (en) | 2013-10-03 | 2019-06-18 | Modernatx, Inc. | Polynucleotides encoding low density lipoprotein receptor |
| EP3092309B1 (en) | 2014-01-10 | 2021-02-24 | Medicago Inc. | Cpmv enhancer elements |
| SG10201912038TA (en) | 2014-04-23 | 2020-02-27 | Modernatx Inc | Nucleic acid vaccines |
| US11273127B2 (en) | 2014-05-06 | 2022-03-15 | The Regents Of The University Of Colorado, A Body Corporate | Compositions, methods and uses for thermally stable multi-targeted antigens |
| WO2015196150A2 (en) | 2014-06-20 | 2015-12-23 | Wisconsin Alumni Research Foundation (Warf) | Mutations that confer genetic stability to additional genes in influenza viruses |
| AU2015290575A1 (en) * | 2014-07-18 | 2017-03-02 | Km Biologics Co., Ltd. | Vaccine containing virus-like particles |
| JP2018504412A (ja) | 2015-01-23 | 2018-02-15 | アイカーン スクール オブ メディシン アット マウント サイナイ | インフルエンザウイルスワクチン接種レジメン |
| KR101745472B1 (ko) | 2015-04-15 | 2017-06-12 | 대한민국 | 조류인플루엔자 바이러스의 세포 감염을 저해하는 물질을 선별하는 방법 |
| WO2016183292A1 (en) | 2015-05-14 | 2016-11-17 | Longhorn Vaccines And Diagnostics, Llc | Rapid methods for the extraction of nucleic acids from biological samples |
| JP2018530314A (ja) * | 2015-08-28 | 2018-10-18 | ウィスコンシン アルムニ リサーチ ファンデイション | ウイルス様粒子からの感染性インフルエンザウイルスの生成 |
| WO2017041100A2 (en) | 2015-09-03 | 2017-03-09 | Novavax, Inc. | Vaccine compositions having improved stability and immunogenicity |
| US10391163B2 (en) * | 2015-09-10 | 2019-08-27 | Academia Sinica | Bird flu vaccine combination comprising virus-like particles and novel adjuvants |
| EP3374496A4 (en) | 2015-11-13 | 2019-07-10 | PDS Biotechnology Corporation | LIPIDES AS SYNTHETIC VECTORS FOR IMPROVING ANTIGEN PROCESSING AND PRESENTATION EX-VIVO IN DENDRITIC CELL THERAPY |
| AU2017221444B2 (en) | 2016-02-19 | 2021-07-29 | Wisconsin Alumni Research Foundation (Warf) | Improved influenza B virus replication for vaccine development |
| EP3471767A4 (en) | 2016-06-15 | 2020-01-15 | Icahn School of Medicine at Mount Sinai | Influenza virus hemagglutinin proteins and uses thereof |
| US11254733B2 (en) | 2017-04-07 | 2022-02-22 | Icahn School Of Medicine At Mount Sinai | Anti-influenza B virus neuraminidase antibodies and uses thereof |
| CA3063723A1 (en) | 2017-05-18 | 2018-11-22 | Modernatx, Inc. | Polynucleotides encoding tethered interleukin-12 (il12) polypeptides and uses thereof |
| DK3661968T3 (da) | 2017-08-04 | 2022-06-20 | Hospital For Sick Children | Nanopartikelplatform til antistof- og vaccineindgivelse |
| CN107353328A (zh) * | 2017-08-23 | 2017-11-17 | 上海市动物疫病预防控制中心 | 一种重组的h9n2亚型禽流感病毒样颗粒及其制备方法和用途 |
| JP2021500891A (ja) | 2017-10-25 | 2021-01-14 | ウィスコンシン アルムニ リサーチ ファンデイション | 卵における複製のための安定化されたhaを有する組換えインフルエンザウイルス |
| CN110575538B (zh) * | 2018-06-11 | 2022-08-19 | 普莱柯生物工程股份有限公司 | 一种禽流感病毒样颗粒疫苗、及其制备方法和应用 |
| EP3810634A4 (en) | 2018-06-21 | 2022-07-27 | Icahn School of Medicine at Mount Sinai | Mosaic influenza virus hemagglutinin polypeptides and uses thereof |
| US12343390B2 (en) | 2018-08-07 | 2025-07-01 | Wisconsin Alumni Research Foundation (Warf) | Recombinant biologically contained filovirus vaccine |
| JP7655849B2 (ja) | 2018-08-20 | 2025-04-02 | ウィスコンシン アルムニ リサーチ ファンデイション | ヘマグルチニン(ha)タンパク質内の非ドミナントエピトープに対する免疫応答を誘起するためのベクター |
| CN109097341B (zh) * | 2018-08-28 | 2021-09-24 | 青岛农业大学 | 一种同时表达ha和hef的复制缺陷型重组流感病毒 |
| WO2020051766A1 (zh) | 2018-09-11 | 2020-03-19 | 上海市公共卫生临床中心 | 一种广谱抗流感疫苗免疫原及其应用 |
| US11851648B2 (en) | 2019-02-08 | 2023-12-26 | Wisconsin Alumni Research Foundation (Warf) | Humanized cell line |
| WO2020223699A1 (en) | 2019-05-01 | 2020-11-05 | Wisconsin Alumni Research Foundation (Warf) | Improved influenza virus replication for vaccine development |
| US11807872B2 (en) | 2019-08-27 | 2023-11-07 | Wisconsin Alumni Research Foundation (Warf) | Recombinant influenza viruses with stabilized HA for replication in eggs |
| WO2021150874A1 (en) | 2020-01-24 | 2021-07-29 | Wisconsin Alumni Research Foundation (Warf) | Recombinant influenza viruses with stabilized na |
| US12290562B2 (en) | 2020-03-25 | 2025-05-06 | Wisconsin Alumni Research Foundation (Warf) | Recombinant multivalent influenza viruses |
| CN113403343A (zh) * | 2021-04-30 | 2021-09-17 | 吉林大学 | 一种h3n2与h9n2亚型禽流感二价嵌合型病毒样颗粒的制备 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4552758A (en) * | 1983-12-20 | 1985-11-12 | St. Jude Children's Research Hospital | Human use of avian-human reassortants as vaccines for influenza A virus |
| US4911928A (en) | 1987-03-13 | 1990-03-27 | Micro-Pak, Inc. | Paucilamellar lipid vesicles |
| US5762939A (en) | 1993-09-13 | 1998-06-09 | Mg-Pmc, Llc | Method for producing influenza hemagglutinin multivalent vaccines using baculovirus |
| CA2183435C (en) | 1994-02-24 | 2008-05-13 | Craig D. Wright | Vaccines containing paucilamellar lipid vesicles as immunological adjuvants |
| AU3760495A (en) | 1994-09-30 | 1996-04-26 | Aviron | Chimeric Influenza Virus And Electroporation Method |
| TW570803B (en) | 1997-04-09 | 2004-01-11 | Duphar Int Res | Influenza vaccine |
| US5945489A (en) | 1997-09-19 | 1999-08-31 | Ashland, Inc. | Liquid oligomers containing unsaturation |
| US6224882B1 (en) | 1997-11-07 | 2001-05-01 | Protein Science Corp. | Insect cells or fractions as adjuvant for antigens |
| US6544785B1 (en) * | 1998-09-14 | 2003-04-08 | Mount Sinai School Of Medicine Of New York University | Helper-free rescue of recombinant negative strand RNA viruses |
| US8715940B2 (en) * | 1999-04-06 | 2014-05-06 | Wisconsin Alumni Research Foundation | Method of making recombinant influenza virus |
| MXPA02012254A (es) | 2000-06-23 | 2003-04-25 | American Cyanamid Co | Montaje de particulas quimericas semejantes al virus de influenza y del tipo silvestre. |
| US6513184B1 (en) | 2000-06-28 | 2003-02-04 | S. C. Johnson & Son, Inc. | Particle entrapment system |
| EP1465987B1 (en) * | 2001-12-07 | 2008-01-23 | Crucell Holland B.V. | Production of viruses, viral isolates and vaccines |
| AU2002359792A1 (en) | 2001-12-18 | 2003-06-30 | Proteologics, Inc. | Methods and compositions for the inhibition of viral release |
| US8506967B2 (en) | 2003-07-11 | 2013-08-13 | Novavax, Inc. | Functional influenza virus like particles (VLPs) |
| US8551756B2 (en) | 2003-07-11 | 2013-10-08 | Novavax, Inc. | Avian influenza chimeric VLPS |
| US8080255B2 (en) | 2003-07-11 | 2011-12-20 | Novavax Inc. | Functional influenza virus like particles (VLPs) |
| US8992939B2 (en) * | 2003-07-11 | 2015-03-31 | Novavax, Inc. | Highly efficient influenza matrix (M1) proteins |
| US8592197B2 (en) * | 2003-07-11 | 2013-11-26 | Novavax, Inc. | Functional influenza virus-like particles (VLPs) |
-
2003
- 2003-07-11 US US10/617,569 patent/US8592197B2/en not_active Expired - Lifetime
-
2004
- 2004-07-09 PL PL04786052T patent/PL1644037T3/pl unknown
- 2004-07-09 DK DK04786052.3T patent/DK1644037T3/da active
- 2004-07-09 DK DK10010286.2T patent/DK2343084T3/da active
- 2004-07-09 SG SG201006667-8A patent/SG165378A1/en unknown
- 2004-07-09 WO PCT/US2004/022001 patent/WO2005020889A2/en not_active Ceased
- 2004-07-09 CN CN2013100841712A patent/CN103157103A/zh active Pending
- 2004-07-09 EP EP10010286.2A patent/EP2343084B1/en not_active Expired - Lifetime
- 2004-07-09 PT PT100102862T patent/PT2343084E/pt unknown
- 2004-07-09 PT PT04786052T patent/PT1644037E/pt unknown
- 2004-07-09 CA CA2532335A patent/CA2532335C/en not_active Expired - Lifetime
- 2004-07-09 CN CNA2004800261523A patent/CN1849134A/zh active Pending
- 2004-07-09 EP EP04786052A patent/EP1644037B1/en not_active Expired - Lifetime
- 2004-07-09 CN CN201710658288.5A patent/CN107513102A/zh active Pending
- 2004-07-09 ES ES10010286T patent/ES2422580T3/es not_active Expired - Lifetime
- 2004-07-09 PL PL10010286T patent/PL2343084T3/pl unknown
- 2004-07-09 NZ NZ544580A patent/NZ544580A/en not_active IP Right Cessation
- 2004-07-09 BR BRPI0412519-3A patent/BRPI0412519A/pt not_active Application Discontinuation
- 2004-07-09 SG SG200701731-2A patent/SG131116A1/en unknown
- 2004-07-09 AU AU2004268510A patent/AU2004268510B2/en not_active Expired
- 2004-07-09 JP JP2006518925A patent/JP2007529997A/ja not_active Withdrawn
- 2004-07-09 RU RU2006104113/13A patent/RU2369405C2/ru active
- 2004-07-09 AT AT04786052T patent/ATE538810T1/de active
- 2004-07-09 MX MXPA06000469A patent/MXPA06000469A/es active IP Right Grant
- 2004-07-09 ES ES04786052T patent/ES2380021T3/es not_active Expired - Lifetime
- 2004-07-09 KR KR1020067000719A patent/KR101153929B1/ko not_active Expired - Lifetime
-
2006
- 2006-01-10 IL IL173071A patent/IL173071A/en active IP Right Grant
- 2006-03-10 US US11/372,466 patent/US7763450B2/en not_active Expired - Lifetime
-
2010
- 2010-09-16 JP JP2010208644A patent/JP2011046715A/ja active Pending
-
2013
- 2013-03-12 US US13/796,125 patent/US9144607B2/en not_active Expired - Lifetime
- 2013-07-05 JP JP2013141285A patent/JP2013216685A/ja not_active Withdrawn
-
2014
- 2014-12-22 JP JP2014258346A patent/JP2015057447A/ja not_active Withdrawn
-
2015
- 2015-04-30 US US14/700,577 patent/US9474799B2/en not_active Expired - Lifetime
-
2016
- 2016-06-02 JP JP2016110634A patent/JP2016164195A/ja active Pending
- 2016-10-24 US US15/332,330 patent/US9956280B2/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107488216A (zh) * | 2017-09-06 | 2017-12-19 | 中国人民解放军军事医学科学院微生物流行病研究所 | 一种流感病毒通用疫苗及其制备方法与应用 |
| CN107488216B (zh) * | 2017-09-06 | 2020-11-10 | 中国人民解放军军事医学科学院微生物流行病研究所 | 一种流感病毒通用疫苗及其制备方法与应用 |
| CN110240634A (zh) * | 2018-03-08 | 2019-09-17 | 普莱柯生物工程股份有限公司 | 一种禽流感病毒样颗粒疫苗、及其制备方法和应用 |
| CN110240634B (zh) * | 2018-03-08 | 2022-09-06 | 普莱柯生物工程股份有限公司 | 一种禽流感病毒样颗粒疫苗、及其制备方法和应用 |
| CN113461786A (zh) * | 2020-03-30 | 2021-10-01 | 普莱柯生物工程股份有限公司 | 一种禽流感病毒样颗粒疫苗、及其制备方法和应用 |
| CN113461786B (zh) * | 2020-03-30 | 2022-09-13 | 普莱柯生物工程股份有限公司 | 一种禽流感病毒样颗粒疫苗、及其制备方法和应用 |
| CN113398259A (zh) * | 2021-04-30 | 2021-09-17 | 吉林大学 | 一种h9n2亚型禽流感新型标记疫苗的制备方法及其应用 |
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103157103A (zh) | 功能性流感病毒样颗粒(VLPs) | |
| US8080255B2 (en) | Functional influenza virus like particles (VLPs) | |
| EP1937301B1 (en) | Functional influenza virus like particles (vlps) | |
| US8506967B2 (en) | Functional influenza virus like particles (VLPs) | |
| KR20180115717A (ko) | 이종 에피토프 및/또는 성숙 절단 부위를 갖는 인플루엔자 헤마글루티닌 조성물 | |
| AU2011218606B2 (en) | Functional influenza virus-like particles (VLPS) | |
| HK1091118B (en) | Functional influenza virus-like particles (vlps) | |
| HK1109169A (en) | Defective influenza virus particles | |
| HK1194099A (en) | Functional influenza virus like particles (vlps) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130619 |