CN102816763A - 特异性结合于疟原虫乳酸脱氢酶pLDH的DNA适体 - Google Patents
特异性结合于疟原虫乳酸脱氢酶pLDH的DNA适体 Download PDFInfo
- Publication number
- CN102816763A CN102816763A CN2012100151040A CN201210015104A CN102816763A CN 102816763 A CN102816763 A CN 102816763A CN 2012100151040 A CN2012100151040 A CN 2012100151040A CN 201210015104 A CN201210015104 A CN 201210015104A CN 102816763 A CN102816763 A CN 102816763A
- Authority
- CN
- China
- Prior art keywords
- fit
- dna
- seq
- pvldh
- plasmodium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000224016 Plasmodium Species 0.000 title claims abstract description 18
- 108091008102 DNA aptamers Proteins 0.000 title abstract 3
- 102000003855 L-lactate dehydrogenase Human genes 0.000 title abstract 3
- 108700023483 L-lactate dehydrogenases Proteins 0.000 title abstract 3
- 201000004792 malaria Diseases 0.000 claims abstract description 26
- 238000009007 Diagnostic Kit Methods 0.000 claims abstract description 9
- 101710088194 Dehydrogenase Proteins 0.000 claims description 38
- 210000002966 serum Anatomy 0.000 claims description 38
- 241000223810 Plasmodium vivax Species 0.000 claims description 10
- 241000223960 Plasmodium falciparum Species 0.000 claims description 7
- 241001505293 Plasmodium ovale Species 0.000 claims description 4
- 238000003745 diagnosis Methods 0.000 abstract description 8
- 239000000203 mixture Substances 0.000 abstract description 4
- 230000035945 sensitivity Effects 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 57
- 230000009182 swimming Effects 0.000 description 31
- 102000053602 DNA Human genes 0.000 description 28
- 108020004682 Single-Stranded DNA Proteins 0.000 description 28
- 239000002585 base Substances 0.000 description 20
- 238000002337 electrophoretic mobility shift assay Methods 0.000 description 13
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 239000011324 bead Substances 0.000 description 12
- 108090000623 proteins and genes Proteins 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 239000012148 binding buffer Substances 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 230000008859 change Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 239000007983 Tris buffer Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 150000002460 imidazoles Chemical class 0.000 description 4
- 244000045947 parasite Species 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 4
- 239000011782 vitamin Substances 0.000 description 4
- 229940088594 vitamin Drugs 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 125000003275 alpha amino acid group Chemical group 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- 150000003722 vitamin derivatives Chemical class 0.000 description 3
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000002405 diagnostic procedure Methods 0.000 description 2
- 239000012149 elution buffer Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002523 gelfiltration Methods 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 238000003317 immunochromatography Methods 0.000 description 2
- 230000002045 lasting effect Effects 0.000 description 2
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 230000008521 reorganization Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- MEIRRNXMZYDVDW-MQQKCMAXSA-N (2E,4E)-2,4-hexadien-1-ol Chemical compound C\C=C\C=C\CO MEIRRNXMZYDVDW-MQQKCMAXSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 239000004386 Erythritol Substances 0.000 description 1
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 241000408529 Libra Species 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 208000000112 Myalgia Diseases 0.000 description 1
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 1
- 241000978776 Senegalia senegal Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 239000000205 acacia gum Substances 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 229960004676 antithrombotic agent Drugs 0.000 description 1
- -1 antithrombotics Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 235000019414 erythritol Nutrition 0.000 description 1
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 description 1
- 229940009714 erythritol Drugs 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000021384 green leafy vegetables Nutrition 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 235000019359 magnesium stearate Nutrition 0.000 description 1
- 229960002160 maltose Drugs 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 229960001855 mannitol Drugs 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 229960002216 methylparaben Drugs 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 description 1
- 229960003415 propylparaben Drugs 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000003375 selectivity assay Methods 0.000 description 1
- 238000012882 sequential analysis Methods 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000012916 structural analysis Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56905—Protozoa
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
- A61K31/711—Natural deoxyribonucleic acids, i.e. containing only 2'-deoxyriboses attached to adenine, guanine, cytosine or thymine and having 3'-5' phosphodiester links
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/44—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from protozoa
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/904—Oxidoreductases (1.) acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
SEQ ID NO: | 碱基序列 |
1 | GTTCGATTGGATTGTGCCGGAAGTGCTGGCTCGAAC |
2 | GAACTCATTGGCTGGAGGCGGCAGTACCGCTTGAGTTC |
1 | PvLDH |
2 | 适体#2 |
3 | 适体#2+PvLDH |
4 | 适体#1 |
5 | 适体#1+PvLDH |
6 | PfLDH |
7 | 适体#2 |
8 | 适体#2+PfLDH |
9 | 适体#1 |
10 | 适体#1+PfLDH |
适体 | pvLDH的Kd | pfLDH的Kd |
SEQ ID NO:1 | 16.8nM | 38.7nM |
SEQ ID NO:2 | 31.7nM | 49.6nM |
1 | PvLDH |
2、3 | 适体、适体+PvLDH |
4、5 | 环1、环1+PvLDH |
6、7 | 茎、茎+PvLDH |
8、9 | 环2、环2+PvLDH |
Claims (9)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR10-2011-0054796 | 2011-06-07 | ||
KR1020110054796A KR101319202B1 (ko) | 2011-06-07 | 2011-06-07 | pLDH에 특이적으로 결합하는 DNA 압타머 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102816763A true CN102816763A (zh) | 2012-12-12 |
CN102816763B CN102816763B (zh) | 2014-07-16 |
Family
ID=45464463
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201210015104.0A Expired - Fee Related CN102816763B (zh) | 2011-06-07 | 2012-01-17 | 特异性结合于疟原虫乳酸脱氢酶pLDH的DNA适体 |
Country Status (5)
Country | Link |
---|---|
US (1) | US8541561B2 (zh) |
EP (1) | EP2532749B1 (zh) |
JP (1) | JP5524990B2 (zh) |
KR (1) | KR101319202B1 (zh) |
CN (1) | CN102816763B (zh) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106324252A (zh) * | 2016-08-09 | 2017-01-11 | 广东合鑫生物科技有限公司 | 非抗体结合蛋白在制备疟疾检测试剂中的应用 |
WO2019113827A1 (en) * | 2017-12-13 | 2019-06-20 | Versitech Limited | Sandwich and species-specific nucleic acid aptamers against plasmodium lactate dehydrogenase for malaria diagnosis |
CN110951703A (zh) * | 2019-12-23 | 2020-04-03 | 杭州贤至生物科技有限公司 | 一种间日疟原虫乳酸脱氢酶重组蛋白及其单克隆抗体的制备 |
WO2020134306A1 (zh) * | 2018-12-25 | 2020-07-02 | 东莞市朋志生物科技有限公司 | 一种抗泛种特异性疟原虫乳酸脱氢酶的抗体 |
CN113195722A (zh) * | 2018-12-19 | 2021-07-30 | Md保健株式会社 | 与基孔肯雅热病毒e2特异性结合的dna适体及其用途 |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013117162A1 (en) * | 2012-02-09 | 2013-08-15 | The University Of Hong Kong | Nucleic acid aptamers against plasmodium lactate dehydrogenase and histidine-rich protein ii and uses thereof for malaria diagnosis |
TWI482857B (zh) | 2013-10-21 | 2015-05-01 | Nat Univ Tsing Hua | 針對糖化血紅素及血紅素具有高專一性的適合體及其應用 |
TWI480374B (zh) | 2013-10-23 | 2015-04-11 | Nat Univ Tsing Hua | 針對a型流感h1亞型病毒具有高專一性的適合體及其應用 |
KR101698654B1 (ko) | 2014-12-24 | 2017-01-20 | 포항공과대학교 산학협력단 | En2에 특이적으로 결합하는 dna 압타머 및 이의 용도 |
KR101875135B1 (ko) * | 2016-07-04 | 2018-07-06 | 부산대학교 산학협력단 | 교류 전위 변조 마이크로플루이딕 채널을 이용한 압타머 선별 방법 |
CN108004246A (zh) * | 2017-12-25 | 2018-05-08 | 中国人民解放军第四军医大学 | 利用金属亲和法快速进行液相靶标selex筛选的方法 |
CN109280651B (zh) * | 2018-09-13 | 2021-11-26 | 四川自豪时代药业有限公司 | 一种乳酸脱氢酶突变体基因LbLDH1及其在大肠杆菌中高效表达的发酵方法 |
EP3860453A4 (en) | 2018-10-02 | 2022-08-03 | Wearoptimo Pty Ltd | MEASUREMENT SYSTEM |
WO2021180906A1 (en) | 2020-03-13 | 2021-09-16 | Fundació Institut De Bioenginyeria De Catalunya | Aptamers for detecting plasmodium-infected red blood cells |
JP2021167795A (ja) | 2020-04-13 | 2021-10-21 | 国立大学法人大阪大学 | 血液分析装置 |
DE102021100290A1 (de) | 2021-01-11 | 2022-07-14 | Forschungszentrum Jülich GmbH | Biosensor für elektrochemische Detektion von Malaria-Biomarkern |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009075404A1 (en) * | 2007-12-13 | 2009-06-18 | Bioland Ltd. | The kit for diagnosing mixed malaria infection of plasmodium vivax and plasmodium falciparum comprising specific antibodies against lactate dehydrogenase of plasmodium vivax and plasmodium falciparum |
CN101806799A (zh) * | 2009-06-09 | 2010-08-18 | 北京金沃夫生物工程科技有限公司 | 一种快速检测和鉴别恶性疟和间日疟的试剂盒及其制备方法 |
CN101981188A (zh) * | 2008-02-05 | 2011-02-23 | 南方佛斯卡专利公司 | 作为抗疟剂的rna适体的筛选 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20040000235A (ko) | 2002-06-24 | 2004-01-03 | 삼성전자주식회사 | 비디오 모드로의 전환이 간편한 영상신호 수신장치 |
KR20040023526A (ko) * | 2002-09-10 | 2004-03-18 | 주식회사 벤다이아 테크 | 말라리아 진단용 키트 |
KR100982064B1 (ko) * | 2006-03-28 | 2010-09-13 | 원광대학교산학협력단 | 말라리아 원충에 대한 단일클론 항체 및 이를 이용한말라리아의 진단방법 |
-
2011
- 2011-06-07 KR KR1020110054796A patent/KR101319202B1/ko active IP Right Grant
-
2012
- 2012-01-17 CN CN201210015104.0A patent/CN102816763B/zh not_active Expired - Fee Related
- 2012-01-17 EP EP12151425.1A patent/EP2532749B1/en not_active Not-in-force
- 2012-01-20 JP JP2012009760A patent/JP5524990B2/ja not_active Expired - Fee Related
- 2012-01-20 US US13/355,159 patent/US8541561B2/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009075404A1 (en) * | 2007-12-13 | 2009-06-18 | Bioland Ltd. | The kit for diagnosing mixed malaria infection of plasmodium vivax and plasmodium falciparum comprising specific antibodies against lactate dehydrogenase of plasmodium vivax and plasmodium falciparum |
CN101981188A (zh) * | 2008-02-05 | 2011-02-23 | 南方佛斯卡专利公司 | 作为抗疟剂的rna适体的筛选 |
CN101806799A (zh) * | 2009-06-09 | 2010-08-18 | 北京金沃夫生物工程科技有限公司 | 一种快速检测和鉴别恶性疟和间日疟的试剂盒及其制备方法 |
Non-Patent Citations (2)
Title |
---|
SEONGHWAN LEE ET AL: "A highly sensitive aptasensor towards plasmodium lactate dehydrigenase for the diagnosis of malaria", 《BIOSENSORS AND BIOELECTRONICS》 * |
Y.W.CHEUNG AND J.A.TANNER: "New avenues to malaria diagnosis–nucleic acid aptamers against P.falciparum histidine rich protein 2 and lactate dehydrogenase", 《FEBS JOURNAL》 * |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106324252A (zh) * | 2016-08-09 | 2017-01-11 | 广东合鑫生物科技有限公司 | 非抗体结合蛋白在制备疟疾检测试剂中的应用 |
WO2019113827A1 (en) * | 2017-12-13 | 2019-06-20 | Versitech Limited | Sandwich and species-specific nucleic acid aptamers against plasmodium lactate dehydrogenase for malaria diagnosis |
CN113195722A (zh) * | 2018-12-19 | 2021-07-30 | Md保健株式会社 | 与基孔肯雅热病毒e2特异性结合的dna适体及其用途 |
CN113195722B (zh) * | 2018-12-19 | 2023-11-24 | Md保健株式会社 | 与基孔肯雅热病毒e2特异性结合的dna适体及其用途 |
WO2020134306A1 (zh) * | 2018-12-25 | 2020-07-02 | 东莞市朋志生物科技有限公司 | 一种抗泛种特异性疟原虫乳酸脱氢酶的抗体 |
CN110951703A (zh) * | 2019-12-23 | 2020-04-03 | 杭州贤至生物科技有限公司 | 一种间日疟原虫乳酸脱氢酶重组蛋白及其单克隆抗体的制备 |
CN110951703B (zh) * | 2019-12-23 | 2023-04-07 | 杭州贤至生物科技有限公司 | 一种间日疟原虫乳酸脱氢酶重组蛋白及其单克隆抗体的制备 |
Also Published As
Publication number | Publication date |
---|---|
KR20120135842A (ko) | 2012-12-17 |
US20120316325A1 (en) | 2012-12-13 |
CN102816763B (zh) | 2014-07-16 |
JP5524990B2 (ja) | 2014-06-18 |
JP2012254074A (ja) | 2012-12-27 |
KR101319202B1 (ko) | 2013-10-23 |
US8541561B2 (en) | 2013-09-24 |
EP2532749A1 (en) | 2012-12-12 |
EP2532749B1 (en) | 2015-06-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102816763B (zh) | 特异性结合于疟原虫乳酸脱氢酶pLDH的DNA适体 | |
Rich et al. | Grading the commercial optical biosensor literature—Class of 2008:‘The Mighty Binders’ | |
EP2532748B1 (en) | DNA aptamer specifically binding to human cardiac troponin I | |
WO2017040813A2 (en) | Detection of gene loci with crispr arrayed repeats and/or polychromatic single guide ribonucleic acids | |
US20130224779A1 (en) | Method for large scale preparation of the active domain of human protein tyrosine phosphatase without fusion protein | |
KR101964746B1 (ko) | dCas9 단백질 및 표적 핵산 서열에 결합하는 gRNA를 이용한 핵산 검출의 민감도 및 특이도 향상용 조성물 및 방법 | |
RU2009135270A (ru) | Способы выявления воспалительного заболевания кишечника | |
Cacciò et al. | Multilocus sequence typing of Dientamoeba fragilis identified a major clone with widespread geographical distribution | |
KR102016668B1 (ko) | 치쿤군야 바이러스 e2에 특이적으로 결합하는 dna 압타머 및 이의 용도 | |
JP2010006788A (ja) | 抗体複合体、抗原検出方法、及び抗体複合体製造方法 | |
KR101923198B1 (ko) | Tb7.7에 특이적으로 결합하는 dna 압타머 및 이의 용도 | |
JP2006510371A (ja) | RNA:DNAハイブリッドを捕捉、検出及び定量する方法、及びその中で有用な修飾されたRNaseH | |
Bannantine et al. | Monoclonal antibodies bind a SNP-sensitive epitope that is present uniquely in Mycobacterium avium subspecies paratuberculosis | |
CN113227378B (zh) | 与登革病毒ediii特异性结合的dna适配体及其用途 | |
CN102766633A (zh) | 可用于检测人肝癌细胞株Bel-7404的核酸适体及其筛选方法和应用 | |
KR101351647B1 (ko) | 인간 심근 트로포닌 ⅰ에 특이적으로 결합하는 dna 압타머 | |
KR20170056126A (ko) | 스핑크1 단백질에 특이적으로 결합하는 dna 앱타머 및 이의 용도 | |
KR20120071191A (ko) | 표적 핵산의 염기 서열을 결정하기 위한 키트 및 이를 이용한 표적 핵산의 염기 서열을 결정하는 방법 | |
JP2017160272A5 (zh) | ||
Hu et al. | Development and characterization of two monoclonal antibodies against grouper iridovirus 55L and 97L proteins | |
KR101989679B1 (ko) | 노닐페놀 에톡실레이트에 특이적으로 결합하는 dna 압타머 및 이의 용도 | |
CN111349631A (zh) | 与鳍藻毒素-1特异性结合的适配体及其应用 | |
Peng et al. | Expression, immunolocalization and serodiagnostic value of a myophilin-like protein from Schistosoma japonicum | |
KR101432897B1 (ko) | 제초제 저항성 pat 단백질에 특이적으로 결합하는 dna 앱타머 및 이의 용도 | |
CN105112377B (zh) | 一株分泌抗p53单克隆抗体的杂交瘤细胞株及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20170119 Address after: Seoul special city Patentee after: Pan Cangyi Address before: Gyeongbuk, South Korea Patentee before: Postech Academy-Industry Foundation |
|
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20170224 Address after: Seoul special city Patentee after: MD healthcare Address before: Seoul special city Patentee before: Pan Cangyi |
|
TR01 | Transfer of patent right |
Effective date of registration: 20181025 Address after: Seoul special city Patentee after: MD auto Co. Address before: Seoul special city Patentee before: MD healthcare |
|
TR01 | Transfer of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140716 |
|
CF01 | Termination of patent right due to non-payment of annual fee |