CN102712895B - 无甘油的乙醇发酵生产 - Google Patents

无甘油的乙醇发酵生产 Download PDF

Info

Publication number
CN102712895B
CN102712895B CN201080042861.6A CN201080042861A CN102712895B CN 102712895 B CN102712895 B CN 102712895B CN 201080042861 A CN201080042861 A CN 201080042861A CN 102712895 B CN102712895 B CN 102712895B
Authority
CN
China
Prior art keywords
cell
gene
nucleic acid
seq
nad
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201080042861.6A
Other languages
English (en)
Chinese (zh)
Other versions
CN102712895A (zh
Inventor
雅各布斯·托马斯·普若克
安东尼斯·J·A·范马里斯
维克多·加布里埃尔·格达拉普梅迪纳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=41460190&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN102712895(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Publication of CN102712895A publication Critical patent/CN102712895A/zh
Application granted granted Critical
Publication of CN102712895B publication Critical patent/CN102712895B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01008—Glycerol-3-phosphate dehydrogenase (NAD+) (1.1.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01—Acid-Thiol Ligases (6.2.1)
    • C12Y602/01001—Acetate-CoA ligase (6.2.1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201080042861.6A 2009-07-24 2010-07-23 无甘油的乙醇发酵生产 Active CN102712895B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP09166360.9 2009-07-24
EP09166360A EP2277989A1 (en) 2009-07-24 2009-07-24 Fermentative glycerol-free ethanol production
PCT/NL2010/050475 WO2011010923A1 (en) 2009-07-24 2010-07-23 Fermentative glycerol-free ethanol production

Publications (2)

Publication Number Publication Date
CN102712895A CN102712895A (zh) 2012-10-03
CN102712895B true CN102712895B (zh) 2018-08-10

Family

ID=41460190

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201080042861.6A Active CN102712895B (zh) 2009-07-24 2010-07-23 无甘油的乙醇发酵生产

Country Status (15)

Country Link
US (10) US8795998B2 (enExample)
EP (4) EP2277989A1 (enExample)
JP (1) JP5836271B2 (enExample)
CN (1) CN102712895B (enExample)
AU (1) AU2010275085A1 (enExample)
BR (1) BR112012001642B1 (enExample)
CA (1) CA2768897C (enExample)
DK (1) DK2456851T3 (enExample)
EA (1) EA201270187A1 (enExample)
ES (1) ES2706653T3 (enExample)
MX (1) MX2012001062A (enExample)
PL (1) PL2456851T3 (enExample)
UA (1) UA107467C2 (enExample)
WO (1) WO2011010923A1 (enExample)
ZA (1) ZA201200703B (enExample)

Families Citing this family (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2277989A1 (en) * 2009-07-24 2011-01-26 Technische Universiteit Delft Fermentative glycerol-free ethanol production
US9605269B2 (en) * 2010-05-05 2017-03-28 Lallemand Hungary Liquidity Management Llc Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate
WO2011149353A1 (en) * 2010-05-27 2011-12-01 C5 Yeast Company B.V. Yeast strains engineered to produce ethanol from acetic acid and glycerol
CN103124783A (zh) 2010-06-03 2013-05-29 马斯科马公司 表达用于使用淀粉和纤维素进行联合生物加工的糖分解酶的酵母
CN103649321B (zh) 2011-04-05 2019-07-16 拉勒曼德匈牙利流动性管理有限责任公司 用于通过添加交替电子受体改善微生物中的产品收率和产量的方法
US9890398B2 (en) 2011-10-24 2018-02-13 Toyota Jidosha Kabushiki Kaisha Method for producing ethanol using recombinant yeast
DK2776571T3 (en) * 2011-11-09 2017-06-26 Amyris Inc PREPARATION OF ACETYL-COENZYM A-DERIVED ISOPRENOIDS
CA2857498A1 (en) * 2011-11-30 2013-09-26 Mascoma Corporation Engineering an increase in ethanol production by altering cofactor specificity
EP3321368A3 (en) * 2011-11-30 2018-05-23 DSM IP Assets B.V. Yeast strains engineered to produce ethanol from acetic acid and glycerol
IN2015DN01042A (enExample) * 2012-08-28 2015-06-26 Dsm Ip Assets Bv
IN2015DN01041A (enExample) * 2012-08-28 2015-06-26 Dsm Ip Assets Bv
CN104797708A (zh) 2012-08-29 2015-07-22 拉勒曼德匈牙利流动性管理有限责任公司 酵母中用于木质纤维素衍生的寡聚物cbp的酶的表达
US20140256011A1 (en) 2012-11-09 2014-09-11 Mascoma Corporation Method for Acetate Consumption During Ethanolic Fermentaion of Cellulosic Feedstocks
AU2014219510A1 (en) 2013-02-22 2015-08-27 Technische Universiteit Delft Recombinant micro-organism for use in method with increased product yield
JP6087854B2 (ja) * 2013-02-27 2017-03-01 トヨタ自動車株式会社 組換え酵母を用いたエタノールの製造方法
WO2014160050A1 (en) 2013-03-14 2014-10-02 Butamax Advanced Biofuels Llc Glycerol 3- phosphate dehydrogenase for butanol production
CN105209600A (zh) * 2013-03-15 2015-12-30 拉勒曼德匈牙利流动管理有限责任公司 通过代谢工程化的酵母菌株调节由玉米生产乙醇期间的氮代谢的方法
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
WO2014180820A2 (en) * 2013-05-08 2014-11-13 Dsm Ip Assets B.V. Gpd- yeast strains with improved osmotolerance
MX377110B (es) 2013-08-15 2025-03-07 Danstar Ferment Ag Metodos para la mejora del rendimiento y produccion de producto en un microorganismo a traves del reciclaje de glicerol.
AR097480A1 (es) 2013-08-29 2016-03-16 Dsm Ip Assets Bv Células de levadura convertidoras de glicerol y ácido acético con una conversión de ácido acético mejorada
CN105722969A (zh) 2013-10-28 2016-06-29 丹尼斯科美国公司 大规模基因工程化活性干酵母
KR102255306B1 (ko) * 2013-11-15 2021-05-25 삼성전자주식회사 아세트알데히드 데히드로게나제를 포함하는 락테이트 생산능을 갖는 유전적으로 조작된 효모 세포, 그를 제조하는 방법 및 그를 사용하여 락테이트를 생산하는 방법
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
WO2015143317A1 (en) * 2014-03-21 2015-09-24 Novozymes A/S Processes of producing ethanol using a fermenting organism
BR112016021857B1 (pt) 2014-03-28 2024-03-12 Danisco Us Inc Célula de levedura recombinante e método para produzir etanol a partir de glicose
KR20160012561A (ko) * 2014-07-24 2016-02-03 삼성전자주식회사 Erg5의 활성이 증가되도록 유전적으로 조작된, 내산성을 갖는 효모 세포 및 그를 이용하여 락테이트를 생산하는 방법
AU2016235802B2 (en) 2015-03-20 2022-03-31 Microbiogen Pty. Ltd. Processes for producing ethanol and ethanol producing yeast
MY186983A (en) 2015-05-22 2021-08-26 Dsm Ip Assets Bv Acetate consuming yeast cell
EP3359655B1 (en) * 2015-10-06 2019-11-20 DSM IP Assets B.V. Eukaryotic cell with increased production of fermentation product
WO2017189421A1 (en) * 2016-04-28 2017-11-02 Danisco Us Inc. Redox balancing in yeast
CN109312296B (zh) * 2016-06-14 2023-05-05 帝斯曼知识产权资产管理有限公司 重组酵母细胞
JP6447583B2 (ja) * 2016-06-24 2019-01-09 トヨタ自動車株式会社 組換え酵母、及びそれを用いたエタノールの製造方法
CN110177801B (zh) 2016-11-09 2024-02-23 丹尼斯科美国公司 具有改善的醇生产的酵母
CA3047268A1 (en) 2016-12-16 2018-06-21 Danisco Us Inc. Bifunctional phosphoketolase-phosphotransacetylase fusion polypeptides
US10913928B2 (en) 2016-12-20 2021-02-09 Dsm Ip Assets B.V. Yeast strains for ethanol production
CN110382520A (zh) 2017-01-18 2019-10-25 丹尼斯科美国公司 过表达dna聚合酶亚基的经修饰的酵母细胞
US20200377559A1 (en) 2017-03-24 2020-12-03 Danisco Us Inc Reduction of acetate and glycerol in modified yeast having an exogenous ethanol-producing pathway
CN110678545B (zh) 2017-04-17 2023-10-10 丹尼斯科美国公司 减少酵母中乙酸产生的选择的乙酰辅酶a合酶
US12398362B2 (en) 2017-06-06 2025-08-26 Danisco Us Inc. Yeast with improved alcohol production
CA3064143A1 (en) 2017-06-13 2018-12-20 Dsm Ip Assets B.V. Recombinant yeast cell
JP6879111B2 (ja) 2017-08-02 2021-06-02 トヨタ自動車株式会社 組換え酵母及びこれを用いたエタノールの製造方法
UY37835A (es) 2017-08-08 2019-03-29 Danisco Us Inc Producción incrementada de etanol por levaduras que albergan alelos del activador de transcripción constitutivo mal
EP3676288A1 (en) 2017-08-29 2020-07-08 Danisco US Inc. Modified yeast comprising glucose-specific, atp-mediated transporters
CN111133111A (zh) * 2017-09-26 2020-05-08 帝斯曼知识产权资产管理有限公司 消耗乙酸的菌株
WO2019063507A1 (en) 2017-09-26 2019-04-04 Dsm Ip Assets B.V. IMPROVED PROCESS FOR THE PRODUCTION OF ETHANOL
CA3077115A1 (en) * 2017-09-29 2019-04-04 Dsm Ip Assets B.V. Improved glycerol free ethanol production
CA3080147A1 (en) 2017-10-24 2019-05-02 Danisco Us Inc Yeast with improved alcohol production
WO2019110491A1 (en) 2017-12-08 2019-06-13 Dsm Ip Assets B.V. Recombinant yeast cell
WO2019110492A1 (en) 2017-12-08 2019-06-13 Dsm Ip Assets B.V. Recombinant yeast cell
MX2020008309A (es) * 2018-02-15 2020-10-14 Novozymes As Levadura mejorada para produccion de etanol.
HUE065696T2 (hu) 2018-03-06 2024-06-28 Danisco Us Inc Acetát-termelés csökkentése PAB1-et túltermelõ élesztõvel
WO2019173217A1 (en) 2018-03-06 2019-09-12 Danisco Us Inc Compositions and methods for increasing ethanol production by yeast using gcy1 and dak1
US12139704B2 (en) 2018-03-06 2024-11-12 Danisco Us Inc. Yeast with improved alcohol production under high dissolved solids conditions
US20210032642A1 (en) 2018-04-06 2021-02-04 Danisco Us Inc. Increased alcohol production from yeast producing an increased amount of active hac1 protein
UY38179A (es) 2018-04-12 2019-11-29 Danisco Us Inc Levadura que sobreexpresa proteína fosfatasas asociadas con la vía hog
US12209236B2 (en) 2018-05-25 2025-01-28 Danisco Us Inc. Overexpression of fumarate reductase results in an increased fermentation rate in yeast
HUE068875T2 (hu) 2018-05-30 2025-01-28 Danisco Us Inc A GIS1 transzkripciós aktivátor/represszor fokozott expressziója élesztõben megemelkedett etanolelõállításhoz
CN112752845A (zh) 2018-07-27 2021-05-04 丹尼斯科美国公司 来自生产增加量的活性crz1蛋白的酵母的增加的醇生产
US20210395756A1 (en) 2018-09-28 2021-12-23 Danisco Us Inc Over expression of ribonucleotide reductase inhibitor in yeast for increased ethanol production
US20210332091A1 (en) 2018-09-28 2021-10-28 Danisco Us Inc Over-expression of gds1 in yeast for increased ethanol and decreased acetate production
US20210388397A1 (en) 2018-09-28 2021-12-16 Danisco Us Inc Selected phosphotransacetylase genes for increased ethanol production in engineered yeast
BR112021013257A2 (pt) 2019-01-08 2021-09-14 Danisco Us Inc. Levedura híbrida com produção de etanol aumentada
EP3938519A1 (en) 2019-03-14 2022-01-19 Danisco US Inc. Over-expression of fumarate-succinate transporter in yeast for increased ethanol and reduced acetate production
BR112021018135A2 (pt) 2019-03-14 2021-11-16 Danisco Us Inc Superexpressão do citocromo b2 em levedura para produção aumentada de etanol
WO2020263732A1 (en) 2019-06-24 2020-12-30 Danisco Us Inc Disruption of cdc42 effectors in yeast for increased alcohol and lysine production
CA3144620A1 (en) 2019-06-24 2020-12-30 Danisco Us Inc Modified yeast and method for increasing lysine content in fermentation co-products
CA3145108A1 (en) 2019-06-28 2020-12-30 Jaclyn Diana DEMARTINI Modified yeast cells that over-express selected endogenous proteins
BR112022001828A2 (pt) 2019-08-01 2022-06-28 Danisco Us Inc Superexpressão de pho13 para produção de etanol aumentada por levedura
WO2021022097A1 (en) 2019-08-01 2021-02-04 Danisco Us Inc Over-expression of adh5p for increased ethanol production by yeast
MX2022002310A (es) 2019-08-29 2022-06-02 Danisco Us Inc Expresión de beta-glucosidasa en levadura para producción mejorada de etanol.
EP4055171A1 (en) 2019-11-08 2022-09-14 DSM IP Assets B.V. Process for producing ethanol
BR112022010168A2 (pt) 2019-11-26 2022-08-09 Danisco Us Inc Células de levedura modificadas e método para diminuir a produção de acetato
US20230116556A1 (en) 2020-01-23 2023-04-13 Danisco Us Inc. Increased ethanol production by overexpression of jid1 in yeast
US20230374443A1 (en) 2020-10-05 2023-11-23 Dsm Ip Assets B.V. Saccharomyces yeast cell and fermentation process using such
AR125830A1 (es) 2021-05-10 2023-08-16 Danisco Us Inc Producción de etanol aumentada mediante sobreexpresión de kgd2 en levaduras
WO2023285280A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
MX2024000606A (es) 2021-07-12 2024-01-30 Danisco Us Inc Celula de levadura recombinante.
EP4370692A1 (en) 2021-07-12 2024-05-22 Danisco Us Inc Recombinant yeast cell
MX2024000513A (es) 2021-07-12 2024-03-27 Danisco Us Inc Celula de levadura recombinante.
CN117916381A (zh) 2021-07-12 2024-04-19 丹尼斯科美国公司 重组酵母细胞
US20250333768A1 (en) 2021-07-12 2025-10-30 Danisco Us Inc. Recombinant yeast cell
WO2023076323A1 (en) 2021-10-26 2023-05-04 Danisco Us Inc. Reduction in acetate produced by yeast with reduced expression of rsf2 or tda9
US20240409965A1 (en) 2021-11-04 2024-12-12 Danisco Us Inc. Variant polypeptide and recombinant yeast cell
US20240425888A1 (en) 2021-11-04 2024-12-26 Danisco Us Inc. Process for the production of ethanol and recombinant yeast cell
EP4426824A1 (en) 2021-11-04 2024-09-11 Danisco US Inc. Recombinant yeast cell
WO2023208762A2 (en) 2022-04-25 2023-11-02 Dsm Ip Assets B.V. Mutant yeast cell and process for the production of ethanol
WO2024123691A1 (en) 2022-12-05 2024-06-13 Danisco Us Inc. Increased ethanol production by yeast in high dissolved solids
WO2024206342A1 (en) 2023-03-27 2024-10-03 Danisco Us Inc. Reduced fermentation glycerol and acetate using yeast expressing rubisco from gallionella capsiferriformans

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009013159A2 (en) * 2007-07-23 2009-01-29 Dsm Ip Assets B.V. Acetyl-coa producing enzymes in yeast

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7041814B1 (en) 1998-02-18 2006-05-09 Genome Therapeutics Corporation Nucleic acid and amino acid sequences relating to Enterobacter cloacae for diagnostics and therapeutics
US6562958B1 (en) 1998-06-09 2003-05-13 Genome Therapeutics Corporation Nucleic acid and amino acid sequences relating to Acinetobacter baumannii for diagnostics and therapeutics
AU4897999A (en) * 1998-07-10 2000-02-01 Jens Nielsen Metabolically engineered microbial cell comprising a modified redox activity
US6476209B1 (en) 2000-11-28 2002-11-05 Genesis Research & Development Corporation Ltd. Polynucleotides, materials incorporating them, and methods for using them
US7026463B2 (en) 1999-08-13 2006-04-11 Matthew Glenn Polynucleotides and polypeptides, materials incorporating them and methods for using them
FR2807446B1 (fr) 2000-04-11 2005-05-06 Agronomique Inst Nat Rech Genomes de lactococcus lactis, polypeptides et utilisations
EP1355931A2 (en) 2000-10-06 2003-10-29 Novozymes Biotech, Inc. Methods for monitoring multiple gene expression
EP1379549A2 (fr) 2001-02-07 2004-01-14 Institut Pasteur Sequence du genome de photorhabdus luminescens souche tto1 et utilisations
SE0200857D0 (sv) * 2002-03-19 2002-03-19 Forskarpatent I Syd Ab Metabolic engineering for improved xylose utilisation of saccharomyces cerevisiae
US7253001B2 (en) 2002-03-19 2007-08-07 Forskarpatent I Syd Ab Metabolic engineering for improved xylose utilisation of Saccharomyces cerevisiae
JP4372408B2 (ja) 2002-11-14 2009-11-25 三菱レイヨン株式会社 ロドコッカス（Ｒｈｏｄｏｃｏｃｃｕｓ）属細菌組換え体、及びそれを用いた光学活性体の製造方法
GB0227435D0 (en) * 2002-11-25 2002-12-31 Univ Denmark Tech Dtu Metabolically engineered micro-organisms having reduced production of undesired metobolic products
WO2004085627A1 (en) 2003-03-26 2004-10-07 Forskarpatent I Syd Ab New saccharomyces cerevisiae strains utilizing xylose
PL1781772T3 (pl) 2004-07-16 2017-03-31 Dsm Ip Assets B.V. Metaboliczne modyfikowanie fermentujących ksylozę komórek eukariotycznych
ES2595363T3 (es) 2005-02-18 2016-12-29 J. Craig Venter Institute, Inc. Sepsis asociada a las proteínas y los ácidos nucleicos de meningitis / Escherichia coli
BRPI0614990A2 (pt) 2005-08-19 2011-04-26 Univ California células hospedeiras geneticamente modificadas e uso das mesmas para produção de compostos isoprenóides
NZ566406A (en) 2005-10-26 2012-04-27 Butamax Advanced Biofuels Llc Fermentive production of four carbon alcohols
US20080293101A1 (en) 2006-07-27 2008-11-27 Peters Matthew W Engineered microorganisms for increasing product yield in biotransformations, related methods and systems
EP2097528A2 (en) 2006-10-31 2009-09-09 DSMIP Assets B.V. Butanol production in a eukaryotic cell
EP2060632A1 (en) 2007-10-29 2009-05-20 Technische Universität Berlin Method of modifying a yeast cell for the production of ethanol
SG186646A1 (en) 2007-12-13 2013-01-30 Glycos Biotechnologies Inc Microbial conversion of oils and fatty acids to high-value chemicals
DE102008004253B4 (de) * 2008-01-14 2011-07-28 Butalco Gmbh Gesteigerte Produktion von Acetyl-Coenzym A
US8741652B2 (en) 2008-02-20 2014-06-03 Nagarjuna Fertilizers And Chemicals Limited Genetically transformed microorganisms with simultaneous enhancement of reduction potential and reductive enzyme activities for biomass fermentation
JP5755884B2 (ja) * 2008-03-05 2015-07-29 ジェノマティカ， インコーポレイテッド 第一級アルコールを産生する生物
EP2277989A1 (en) * 2009-07-24 2011-01-26 Technische Universiteit Delft Fermentative glycerol-free ethanol production

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009013159A2 (en) * 2007-07-23 2009-01-29 Dsm Ip Assets B.V. Acetyl-coa producing enzymes in yeast

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Overexpressing GLT1 in gpd 1 delta mutant to improve the production of ehtanol of Saccharomyces cerevisiae;Kong QX et al;《Appl Microbiol Biotechnol》;20061005;第73卷(第6期);1382-1386 *
敲除3-磷酸甘油脱氢酶基因GPD对工业酒精酵母发酵的影响;张国英;《中国优秀硕士学位论文全文数据库 工程科技1辑 B016-18》;20090315(第3期);摘要，第1-10页，23-28页 *
酿酒酵母adh2和ald6双基因缺失突变株的构建;王艳尊 等;《微生物学通报》;20090220;第36卷(第2期);211-216 *

Also Published As

Publication number Publication date
US20220135989A1 (en) 2022-05-05
CN102712895A (zh) 2012-10-03
US12435339B2 (en) 2025-10-07
US20210230614A1 (en) 2021-07-29
US20200299704A1 (en) 2020-09-24
EP2456851A1 (en) 2012-05-30
US20110275130A1 (en) 2011-11-10
US10738317B2 (en) 2020-08-11
EP3476931A1 (en) 2019-05-01
ES2706653T3 (es) 2019-03-29
US11326175B2 (en) 2022-05-10
EA201270187A1 (ru) 2012-09-28
WO2011010923A1 (en) 2011-01-27
AU2010275085A1 (en) 2012-02-23
US20190127745A1 (en) 2019-05-02
EP2456851B1 (en) 2018-10-17
MX2012001062A (es) 2012-03-26
JP5836271B2 (ja) 2015-12-24
US11174489B2 (en) 2021-11-16
PL2456851T3 (pl) 2019-04-30
US20200248194A1 (en) 2020-08-06
DK2456851T3 (en) 2019-02-11
US8795998B2 (en) 2014-08-05
US10533181B2 (en) 2020-01-14
EP3828261A1 (en) 2021-06-02
UA107467C2 (uk) 2015-01-12
EP2277989A1 (en) 2011-01-26
US10883110B2 (en) 2021-01-05
BR112012001642A2 (pt) 2015-06-16
US20210230613A1 (en) 2021-07-29
US20140295514A1 (en) 2014-10-02
US20160312246A1 (en) 2016-10-27
BR112012001642B1 (pt) 2018-09-11
US9528117B2 (en) 2016-12-27
JP2013500006A (ja) 2013-01-07
CA2768897A1 (en) 2011-01-27
CA2768897C (en) 2019-06-11
US20190264217A1 (en) 2019-08-29
US11214810B2 (en) 2022-01-04
ZA201200703B (en) 2012-10-31

Similar Documents

Publication Publication Date Title
CN102712895B (zh) 无甘油的乙醇发酵生产
Medina et al. Elimination of glycerol production in anaerobic cultures of a Saccharomyces cerevisiae strain engineered to use acetic acid as an electron acceptor
CN104126011B (zh) 由乙酸和甘油生产乙醇的工程化酵母菌株
EP2663645B1 (en) Yeast strains engineered to produce ethanol from glycerol
JP5321320B2 (ja) 発酵能力が向上された酵母及びその利用
KR102281701B1 (ko) 아세토인을 제조하는 방법
CN101522886B (zh) 发酵阿拉伯糖的真核细胞的代谢工程改造
JP2015000007A (ja) 組換え酵母、及びそれを用いたエタノールの製造方法
SI24955A (sl) Modificirana 6-fosfofrukto-1-kinaza, ki rekombinantnim celicam kvasovke Saccharomyces cerevisiae omogoča fermentativno uporabo pentoznih sladkorjev
WO2023168233A1 (en) Genetically modified yeast and fermentation processes for the production of 3-hydroxypropionate
HK1238294A1 (en) Method for producing acetoin
HK1238294B (zh) 用於生产乙偶姻的方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
ASS Succession or assignment of patent right

Owner name: DSM IP ASSETS BV

Free format text: FORMER OWNER: TECHNISCHE UNIVERSITEIT DELFT

Effective date: 20150310

C41 Transfer of patent application or patent right or utility model
TA01 Transfer of patent application right

Effective date of registration: 20150310

Address after: Holland Heerlen

Applicant after: DSM IP ASSETS B.V.

Address before: Delft

Applicant before: Technische Universiteit Delft

GR01 Patent grant
GR01 Patent grant