AR125830A1 - Producción de etanol aumentada mediante sobreexpresión de kgd2 en levaduras - Google Patents

Producción de etanol aumentada mediante sobreexpresión de kgd2 en levaduras

Info

Publication number
AR125830A1
AR125830A1 ARP220101239A ARP220101239A AR125830A1 AR 125830 A1 AR125830 A1 AR 125830A1 AR P220101239 A ARP220101239 A AR P220101239A AR P220101239 A ARP220101239 A AR P220101239A AR 125830 A1 AR125830 A1 AR 125830A1
Authority
AR
Argentina
Prior art keywords
yeast cells
cells
modified
kgd2
parental
Prior art date
Application number
ARP220101239A
Other languages
English (en)
Inventor
Min Qi
Yehong Jamie Wang
Quinn Qun Zhu
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of AR125830A1 publication Critical patent/AR125830A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/04—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with a disulfide as acceptor (1.2.4)
    • C12Y102/04002—Oxoglutarate dehydrogenase (succinyl-transferring) (1.2.4.2), i.e. alpha-ketoglutarat dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se describen composiciones y métodos referentes a células de levadura modificadas que sobreexpresan a-cetoglutarato deshidrogenasa (KGD2). Las células de levadura modificadas producen cantidades aumentadas de etanol en comparación con células de levadura parentales por lo demás idénticas. Tales células de levadura son particularmente útiles para la producción de etanol a gran escala a partir de sustratos de almidón. Reivindicación 1: Células de levadura modificadas derivadas de células de levadura parentales, comprendiendo las células modificadas una alteración genética que hace que las células modificadas produzcan una cantidad aumentada de polipéptidos de a-cetoglutarato deshidrogenasa (KGD2) en comparación con las células parentales, en las que las células modificadas producen durante la fermentación una cantidad aumentada de etanol en comparación con la cantidad de etanol producida por células de levadura parentales por lo demás idénticas.
ARP220101239A 2021-05-10 2022-05-10 Producción de etanol aumentada mediante sobreexpresión de kgd2 en levaduras AR125830A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202163186332P 2021-05-10 2021-05-10

Publications (1)

Publication Number Publication Date
AR125830A1 true AR125830A1 (es) 2023-08-16

Family

ID=81854523

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP220101239A AR125830A1 (es) 2021-05-10 2022-05-10 Producción de etanol aumentada mediante sobreexpresión de kgd2 en levaduras

Country Status (4)

Country Link
EP (1) EP4355881A1 (es)
CN (1) CN117396608A (es)
AR (1) AR125830A1 (es)
WO (1) WO2022240839A1 (es)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2060632A1 (en) 2007-10-29 2009-05-20 Technische Universität Berlin Method of modifying a yeast cell for the production of ethanol
EP2277989A1 (en) 2009-07-24 2011-01-26 Technische Universiteit Delft Fermentative glycerol-free ethanol production
US8956851B2 (en) 2011-04-05 2015-02-17 Lallemand Hungary Liquidity Management, LLC Methods for the improvement of product yield and production in a microorganism through the addition of alternate electron acceptors
CN105814191A (zh) 2013-08-15 2016-07-27 拉勒曼德匈牙利流动管理有限责任公司 通过甘油回收改进微生物中的产物产率和生产的方法
EP3122876B1 (en) 2014-03-28 2020-11-25 Danisco US Inc. Altered host cell pathway for improved ethanol production
WO2015167043A1 (ko) * 2014-04-30 2015-11-05 삼성전자 주식회사 증가된 알파-케토글루타레이트 데카르복실라제 활성을 갖는 미생물 및 이를 이용한 1,4-부탄디올 생산방법

Also Published As

Publication number Publication date
CN117396608A (zh) 2024-01-12
WO2022240839A1 (en) 2022-11-17
EP4355881A1 (en) 2024-04-24

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