BR112012001642B1 - células de levedura transgênica e método para preparar etanol a partir de acetato e um carboidrato fermentável - Google Patents
células de levedura transgênica e método para preparar etanol a partir de acetato e um carboidrato fermentável Download PDFInfo
- Publication number
- BR112012001642B1 BR112012001642B1 BR112012001642A BR112012001642A BR112012001642B1 BR 112012001642 B1 BR112012001642 B1 BR 112012001642B1 BR 112012001642 A BR112012001642 A BR 112012001642A BR 112012001642 A BR112012001642 A BR 112012001642A BR 112012001642 B1 BR112012001642 B1 BR 112012001642B1
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- cells
- glycerol
- cell
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- ethanol
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01008—Glycerol-3-phosphate dehydrogenase (NAD+) (1.1.1.8)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
- C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
- C12Y602/01—Acid-Thiol Ligases (6.2.1)
- C12Y602/01001—Acetate-CoA ligase (6.2.1.1)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09166360A EP2277989A1 (en) | 2009-07-24 | 2009-07-24 | Fermentative glycerol-free ethanol production |
| PCT/NL2010/050475 WO2011010923A1 (en) | 2009-07-24 | 2010-07-23 | Fermentative glycerol-free ethanol production |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR112012001642A2 BR112012001642A2 (pt) | 2015-06-16 |
| BR112012001642B1 true BR112012001642B1 (pt) | 2018-09-11 |
Family
ID=41460190
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112012001642A BR112012001642B1 (pt) | 2009-07-24 | 2010-07-23 | células de levedura transgênica e método para preparar etanol a partir de acetato e um carboidrato fermentável |
Country Status (15)
| Country | Link |
|---|---|
| US (10) | US8795998B2 (enExample) |
| EP (4) | EP2277989A1 (enExample) |
| JP (1) | JP5836271B2 (enExample) |
| CN (1) | CN102712895B (enExample) |
| AU (1) | AU2010275085A1 (enExample) |
| BR (1) | BR112012001642B1 (enExample) |
| CA (1) | CA2768897C (enExample) |
| DK (1) | DK2456851T3 (enExample) |
| EA (1) | EA201270187A1 (enExample) |
| ES (1) | ES2706653T3 (enExample) |
| MX (1) | MX2012001062A (enExample) |
| PL (1) | PL2456851T3 (enExample) |
| UA (1) | UA107467C2 (enExample) |
| WO (1) | WO2011010923A1 (enExample) |
| ZA (1) | ZA201200703B (enExample) |
Families Citing this family (89)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2277989A1 (en) | 2009-07-24 | 2011-01-26 | Technische Universiteit Delft | Fermentative glycerol-free ethanol production |
| BR112012028290B1 (pt) * | 2010-05-05 | 2021-02-02 | Lallemand Hungary Liquidity Management Llc. | levedura recombinante, processo para converter biomassa em etanol e meio de fermentação compreendendo dita levedura |
| WO2011149353A1 (en) * | 2010-05-27 | 2011-12-01 | C5 Yeast Company B.V. | Yeast strains engineered to produce ethanol from acetic acid and glycerol |
| EP2576762A4 (en) | 2010-06-03 | 2013-12-04 | Mascoma Corp | YEAST FOR THE EXPRESSION OF SACCHAROLYTIC ENZYMES FOR CONSOLIDATED BIOVERIC PROCESSING USING STARCH AND CELLULOSE |
| CN103649321B (zh) | 2011-04-05 | 2019-07-16 | 拉勒曼德匈牙利流动性管理有限责任公司 | 用于通过添加交替电子受体改善微生物中的产品收率和产量的方法 |
| WO2013061941A1 (ja) | 2011-10-24 | 2013-05-02 | トヨタ自動車株式会社 | 組換え酵母を用いたエタノールの製造方法 |
| CN104039974B (zh) * | 2011-11-09 | 2016-10-12 | 阿迈瑞斯公司 | 乙酰辅酶a衍生的类异戊二烯的生产 |
| US10767196B2 (en) | 2011-11-30 | 2020-09-08 | Enchi Corporation | Engineering an increase in ethanol production by altering cofactor specificity |
| MY169074A (en) * | 2011-11-30 | 2019-02-13 | Dsm Ip Assets Bv | Yeast strains engineered to produce ethanol from acetic acid and glycerol |
| IN2015DN01042A (enExample) * | 2012-08-28 | 2015-06-26 | Dsm Ip Assets Bv | |
| IN2015DN01041A (enExample) * | 2012-08-28 | 2015-06-26 | Dsm Ip Assets Bv | |
| BR112015004513A2 (pt) | 2012-08-29 | 2017-11-21 | Lallemand Hungary Liquidity Man Llc | expressão de enzimas em levedura para cbp de oligômero derivado de lignocelulose |
| CN104903441A (zh) * | 2012-11-09 | 2015-09-09 | 拉勒曼德匈牙利流动管理有限责任公司 | 在纤维素原料的乙醇发酵期间消耗乙酸盐的方法 |
| DK2958986T3 (da) | 2013-02-22 | 2022-11-28 | Dsm Ip Assets Bv | Rekombinant mikroorganisme til anvendelse i en fremgangsmåde med forøget produktudbytte |
| US20160002674A1 (en) * | 2013-02-27 | 2016-01-07 | Toyota Jidosha Kabushiki Kaisha | Method for producing ethanol using recombinant yeast |
| US9441250B2 (en) | 2013-03-14 | 2016-09-13 | Butamax Advanced Biofuels Llc | Glycerol 3- phosphate dehydrogenase for butanol production |
| US9850512B2 (en) | 2013-03-15 | 2017-12-26 | The Research Foundation For The State University Of New York | Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield |
| CA3187926A1 (en) | 2013-03-15 | 2014-09-25 | Lallemand Hungary Liquidity Management Llc | Methods for regulating nitrogen metabolism during the production of ethanol from corn by metabolically engineered yeast strains |
| WO2014180820A2 (en) * | 2013-05-08 | 2014-11-13 | Dsm Ip Assets B.V. | Gpd- yeast strains with improved osmotolerance |
| US11753656B2 (en) | 2013-08-15 | 2023-09-12 | Lallemand Hungary Liquidity Management Llc | Methods for the improvement of product yield and production in a microorganism through glycerol recycling |
| AR097480A1 (es) | 2013-08-29 | 2016-03-16 | Dsm Ip Assets Bv | Células de levadura convertidoras de glicerol y ácido acético con una conversión de ácido acético mejorada |
| US20160264927A1 (en) | 2013-10-28 | 2016-09-15 | Danisco Us Inc. | Large Scale Genetically Engineered Active Dry Yeast |
| KR102255306B1 (ko) * | 2013-11-15 | 2021-05-25 | 삼성전자주식회사 | 아세트알데히드 데히드로게나제를 포함하는 락테이트 생산능을 갖는 유전적으로 조작된 효모 세포, 그를 제조하는 방법 및 그를 사용하여 락테이트를 생산하는 방법 |
| US9951363B2 (en) | 2014-03-14 | 2018-04-24 | The Research Foundation for the State University of New York College of Environmental Science and Forestry | Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects |
| US10036043B2 (en) | 2014-03-21 | 2018-07-31 | Novozymes A/S | Processes for producing ethanol and yeast |
| BR112016021857B1 (pt) | 2014-03-28 | 2024-03-12 | Danisco Us Inc | Célula de levedura recombinante e método para produzir etanol a partir de glicose |
| KR20160012561A (ko) * | 2014-07-24 | 2016-02-03 | 삼성전자주식회사 | Erg5의 활성이 증가되도록 유전적으로 조작된, 내산성을 갖는 효모 세포 및 그를 이용하여 락테이트를 생산하는 방법 |
| AU2016235802B2 (en) * | 2015-03-20 | 2022-03-31 | Microbiogen Pty. Ltd. | Processes for producing ethanol and ethanol producing yeast |
| US11319543B2 (en) | 2015-05-22 | 2022-05-03 | Dsm Ip Assets B.V. | Acetate consuming yeast cell |
| BR112018004548A2 (pt) | 2015-10-06 | 2019-09-17 | Dsm Ip Assets Bv | célula eucariótica com produção aumentada de produto de fermentação |
| EP3448988A1 (en) * | 2016-04-28 | 2019-03-06 | Danisco US Inc. | Redox balancing in yeast |
| CN109312296B (zh) * | 2016-06-14 | 2023-05-05 | 帝斯曼知识产权资产管理有限公司 | 重组酵母细胞 |
| JP6447583B2 (ja) * | 2016-06-24 | 2019-01-09 | トヨタ自動車株式会社 | 組換え酵母、及びそれを用いたエタノールの製造方法 |
| BR112019009415A2 (pt) | 2016-11-09 | 2019-07-30 | Danisco Us Inc | levadura com a produção de álcool melhorada |
| WO2018111792A1 (en) | 2016-12-16 | 2018-06-21 | Danisco Us Inc. | Bifunctional phosphoketolase-phosphotransacetylase fusion polypeptides |
| ES2924108T3 (es) | 2016-12-20 | 2022-10-04 | Dsm Ip Assets Bv | Cepas de levadura mejoradas para la producción de etanol |
| US20190382739A1 (en) | 2017-01-18 | 2019-12-19 | Danisco Us Inc. | Modified yeast cells that overexpress a dna polymerase subunit |
| WO2018176021A1 (en) | 2017-03-24 | 2018-09-27 | Danisco Us Inc | Reduction of acetate and glycerol in modified yeast having an exogenous ethanol-producing pathway |
| CN110678545B (zh) | 2017-04-17 | 2023-10-10 | 丹尼斯科美国公司 | 减少酵母中乙酸产生的选择的乙酰辅酶a合酶 |
| US12398362B2 (en) | 2017-06-06 | 2025-08-26 | Danisco Us Inc. | Yeast with improved alcohol production |
| CA3064143A1 (en) | 2017-06-13 | 2018-12-20 | Dsm Ip Assets B.V. | Recombinant yeast cell |
| JP6879111B2 (ja) | 2017-08-02 | 2021-06-02 | トヨタ自動車株式会社 | 組換え酵母及びこれを用いたエタノールの製造方法 |
| UY37835A (es) | 2017-08-08 | 2019-03-29 | Danisco Us Inc | Producción incrementada de etanol por levaduras que albergan alelos del activador de transcripción constitutivo mal |
| EP3676288A1 (en) | 2017-08-29 | 2020-07-08 | Danisco US Inc. | Modified yeast comprising glucose-specific, atp-mediated transporters |
| US20200283805A1 (en) | 2017-09-26 | 2020-09-10 | Dsm Ip Assets B.V. | Improved process for ethanol production |
| CA3074001A1 (en) | 2017-09-26 | 2019-04-04 | Dsm Ip Assets B.V. | Acetic acid consuming strain |
| BR112020003530A2 (pt) * | 2017-09-29 | 2020-09-01 | Dsm Ip Assets B.V. | produção de etanol livre de glicerol aperfeiçoado |
| BR112020008023A2 (pt) | 2017-10-24 | 2020-10-13 | Danisco Us Inc. | levedura com produção melhorada de álcool |
| WO2019110492A1 (en) | 2017-12-08 | 2019-06-13 | Dsm Ip Assets B.V. | Recombinant yeast cell |
| WO2019110491A1 (en) | 2017-12-08 | 2019-06-13 | Dsm Ip Assets B.V. | Recombinant yeast cell |
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| DK3762499T5 (da) | 2018-03-06 | 2024-08-26 | Danisco Us Inc | Reduktion i acetatproduktion ved hjælp af gær, der overudtrykker pab1 |
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| CN113795503A (zh) | 2019-03-14 | 2021-12-14 | 丹尼斯科美国公司 | 细胞色素b2在酵母中过表达用于增加乙醇生产 |
| CN114302958B (zh) | 2019-06-24 | 2024-12-27 | 丹尼斯科美国公司 | 用于增加发酵联产物中赖氨酸含量的经修饰的酵母和方法 |
| BR112021026259A2 (pt) | 2019-06-24 | 2022-03-03 | Danisco Us Inc | Interrupção de efetores cdc42 em levedura para produção aumentada de álcool e lisina |
| US20220259604A1 (en) | 2019-06-28 | 2022-08-18 | Danisco Us Inc | Modified yeast cells that over-express selected endogenous proteins |
| WO2021022140A1 (en) | 2019-08-01 | 2021-02-04 | Danisco Us Inc | Over-expression of pho13 for increased ethanol production by yeast |
| WO2021022097A1 (en) | 2019-08-01 | 2021-02-04 | Danisco Us Inc | Over-expression of adh5p for increased ethanol production by yeast |
| WO2021041141A1 (en) | 2019-08-29 | 2021-03-04 | Danisco Us Inc | Expression of beta-glucosidase in yeast for improved ethanol production |
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