CN102459579B - 具有增强的光输出的合成的刺虾萤光素酶 - Google Patents

具有增强的光输出的合成的刺虾萤光素酶 Download PDF

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CN102459579B
CN102459579B CN201080019477.4A CN201080019477A CN102459579B CN 102459579 B CN102459579 B CN 102459579B CN 201080019477 A CN201080019477 A CN 201080019477A CN 102459579 B CN102459579 B CN 102459579B
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ogluc
luciferase
luminescence
protein
variants
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CN102459579A (zh
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L·P·昂塞尔
K·V·伍德
M·G·伍德
M·哈尔
P·奥托
G·维杜吉里斯
K·齐默尔曼
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Promega Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201080019477.4A 2009-05-01 2010-05-03 具有增强的光输出的合成的刺虾萤光素酶 Active CN102459579B (zh)

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US17483809P 2009-05-01 2009-05-01
US61/174,838 2009-05-01
PCT/US2010/033449 WO2010127368A1 (en) 2009-05-01 2010-05-03 Synthetic oplophorus luciferases with enhanced light output

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US (7) US8557970B2 (https=)
EP (5) EP2990478B1 (https=)
JP (2) JP6038649B2 (https=)
KR (1) KR101665352B1 (https=)
CN (2) CN102459579B (https=)
AU (1) AU2010242771B2 (https=)
BR (1) BRPI1010019B1 (https=)
CA (1) CA2758572A1 (https=)
DK (4) DK2456864T3 (https=)
ES (5) ES2795287T3 (https=)
IL (3) IL215697B (https=)
LT (1) LT3409764T (https=)
PL (5) PL3409764T3 (https=)
PT (4) PT2456864E (https=)
SG (3) SG175180A1 (https=)
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Families Citing this family (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2795287T3 (es) 2009-05-01 2020-11-23 Promega Corp Luciferasas de Oplophorus sintéticas con mayor emisión de luz
SG10202103336SA (en) 2010-11-02 2021-04-29 Promega Corp Novel coelenterazine substrates and methods of use
CN103180324A (zh) 2010-11-02 2013-06-26 普罗美加公司 腔肠素衍生物及其使用方法
JP5896679B2 (ja) * 2011-03-15 2016-03-30 オリンパス株式会社 オオオバボタル由来ルシフェラーゼ
WO2013078244A1 (en) 2011-11-21 2013-05-30 Promega Corporation Carboxy x rhodamine analogs
BR112014020512B1 (pt) 2012-02-21 2022-07-12 Laboratory Corporation Of America Holdings Métodos e sistemas para a detecção de microorganismos
US10519483B2 (en) 2012-02-21 2019-12-31 Laboratory Corporation Of America Holdings Methods and systems for rapid detection of microorganisms using infectious agents
US20140099654A1 (en) 2012-09-26 2014-04-10 Promega Corporation Real-time monitoring
AU2013359160B2 (en) 2012-12-12 2018-11-15 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
KR102292431B1 (ko) * 2012-12-12 2021-08-20 프로메가 코포레이션 세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식
US10067149B2 (en) * 2012-12-12 2018-09-04 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
JP6511720B2 (ja) * 2013-02-28 2019-05-15 Jnc株式会社 コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法
JP6604938B2 (ja) 2013-03-15 2019-11-13 プロメガ コーポレイション タンパク質を官能基または固体表面に共有結合的に係留するための基質
ES2926463T3 (es) 2013-03-15 2022-10-26 Promega Corp Activación de la bioluminiscencia mediante complementación estructural
US10168323B2 (en) * 2013-03-15 2019-01-01 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
AU2014241191A1 (en) 2013-03-27 2015-10-22 Sample Technologies, Inc. Recombinant phage and bacterial detection methods
JP6040846B2 (ja) 2013-04-08 2016-12-07 Jnc株式会社 セレンテラジン類縁体
CN105531382A (zh) 2013-06-19 2016-04-27 六品科技公司 基于噬菌体的细菌检测法
JP6484987B2 (ja) 2013-10-21 2019-03-20 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
JP6442825B2 (ja) 2013-12-26 2018-12-26 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
EP3099691B1 (en) 2014-01-29 2019-11-20 Promega Corporation Pro-substrates for live cell applications
US9790537B2 (en) 2014-01-29 2017-10-17 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
JP6256118B2 (ja) * 2014-03-11 2018-01-10 Jnc株式会社 エビルシフェラーゼの触媒蛋白質のキメラ遺伝子及びその使用法
JP6265020B2 (ja) * 2014-04-16 2018-01-24 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
US9732373B2 (en) 2014-09-11 2017-08-15 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
JP6814132B2 (ja) * 2014-09-12 2021-01-13 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 内部タンパク質タグ
US11365402B2 (en) 2014-09-12 2022-06-21 Promega Corporation Internal protein tags
WO2016127100A1 (en) * 2015-02-05 2016-08-11 Promega Corporation Luciferase-based thermal shift assays
EP3304078B1 (en) 2015-06-05 2022-08-31 Promega Corporation Cell-permeable, cell-compatible, and cleavable linkers for covalent tethering of functional elements
WO2016210294A1 (en) 2015-06-25 2016-12-29 Promega Corporation Thienopyrrole compounds and uses thereof as inhibitors of oplophorus-derived luciferases
WO2018049241A1 (en) 2016-09-09 2018-03-15 Promega Corporation Dual protected pro-coelenterazine substrates
EP4596707A3 (en) 2016-09-19 2025-10-08 University of Southern California Non-radioactive cytotoxicity assays
WO2018075783A2 (en) 2016-10-20 2018-04-26 President And Fellows Of Harvard College In vitro and cell based assays for measuring the activity of botulinum neurotoxins
JP7200102B2 (ja) 2016-11-01 2023-01-06 プロメガ コーポレイション エネルギー受容体に係留されたセレンテラジン類縁体
EP3548492B1 (en) 2016-12-01 2023-07-05 Promega Corporation Cell impermeable coelenterazine analogues
WO2018125992A1 (en) 2016-12-28 2018-07-05 Promega Corporation Functionalized nanoluc inhibitors
EP3395803A1 (en) 2017-04-28 2018-10-31 Institut Pasteur Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins
CN111315875B (zh) * 2017-10-25 2024-02-20 豪夫迈·罗氏有限公司 改进修饰的/突变的细菌萤光素酶
US20200393446A1 (en) 2017-12-08 2020-12-17 Centre National De La Recherche Scientifique (Cnrs) Bioluminescent screening test for detecting cell cytolysis
EP3775240A1 (en) 2018-03-30 2021-02-17 INVISTA Textiles (U.K.) Limited Method for controlling dissolved oxygen concentration in a continuous aerobic fermentation
WO2019191767A1 (en) 2018-03-30 2019-10-03 Invista Textiles (U.K.) Limited High hydrogen utilization and gas recycle
EP3775182A1 (en) * 2018-03-30 2021-02-17 INVISTA Textiles (U.K.) Limited Materials and methods for biosynthetic manufacture of pimelic acid and utilization of synthetic polypeptides
JP7419260B2 (ja) 2018-05-01 2024-01-22 プロメガ コーポレイション ＮａｎｏＬｕｃ自殺基質としてのセレンテラジン化合物
US11999943B2 (en) 2018-05-02 2024-06-04 Inv Nylon Chemicals Americas, Llc Materials and methods for maximizing biosynthesis through alteration of pyruvate-acetyl-CoA-TCA balance in species of the genera ralstonia and cupriavidus and organisms related thereto
WO2019213118A1 (en) 2018-05-02 2019-11-07 Invista North America S.A.R.L. Methods for controlling pha biosynthesis in cupriavidus or ralstonia
US11442063B2 (en) 2018-05-30 2022-09-13 Promega Corporation Broad spectrum kinase binding agents
EP3802514B1 (en) 2018-06-01 2022-08-03 Promega Corporation Inhibitors of oplophorus luciferase-derived bioluminescent complexes
US10962538B2 (en) 2018-06-14 2021-03-30 The Trustees Of Columbia University In The City Of New York Assays using arrestin recruitment and unmodified receptors
CA3114424A1 (en) 2018-10-03 2020-04-09 Promega Corporation Compositions and methods for stabilizing coelenterazine and analogs and derivatives thereof
JP7703446B2 (ja) 2018-11-28 2025-07-07 プロメガ コーポレイション 反応性ペプチド標識付け
EP3891503A2 (en) 2018-12-04 2021-10-13 Promega Corporation Broad spectrum gpcr binding agents
US11913944B2 (en) 2019-03-20 2024-02-27 Promega Corporation Photoaffinity probes
US11360096B2 (en) 2019-05-16 2022-06-14 Duke University Complex BRET technique for measuring biological interactions
CA3140294A1 (en) 2019-06-21 2020-12-24 Jose S. GIL Methods for producing mutant bacteriophages for the detection of listeria
EP3986956A1 (en) 2019-06-24 2022-04-27 Promega Corporation Modified polyamine polymers for delivery of biomolecules into cells
JP7781741B2 (ja) 2019-08-26 2025-12-08 ラボラトリー コーポレイション オブ アメリカ ホールディングス 組換え繁殖欠損指標バクテリオファージを使用して微生物を検出するためのデバイスおよび方法
CN115052991B (zh) 2019-09-11 2025-08-12 美国控股实验室公司 使用重组感染因子以表达指示亚基来快速检测微生物的方法和系统
CA3158729A1 (en) 2019-11-27 2021-06-03 Virginia Kincaid Multipartite luciferase peptides and polypeptides
JP7745550B2 (ja) 2019-12-10 2025-09-29 プロメガ コーポレイション 多官能性プローブを使用する生物発光検出用組成物及び生物発光検出方法
KR20220133969A (ko) 2020-01-28 2022-10-05 프리라인 테라퓨틱스 리미티드 바이러스 벡터에 대한 중화 항체 역가를 결정하기 위한 개선된 분석법
US20210363565A1 (en) 2020-05-22 2021-11-25 Promega Corporation Enhancement of kinase target engagement
EP4204808A1 (en) 2020-08-28 2023-07-05 Promega Corporation Target engagement assay for ras proteins
WO2022138892A1 (ja) 2020-12-25 2022-06-30 中外製薬株式会社 複数の標的分子と共に複合体を形成し得る候補分子のスクリーニング方法
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US20260077065A1 (en) * 2022-09-15 2026-03-19 The Board Of Trustees Of The Leland Stanford Junior University Compositions and Methods for Assessing Kinase Activity
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CN119709661B (zh) * 2024-12-30 2025-10-10 佰翱得(无锡)新药开发有限公司 一种荧光素酶OLuc突变体及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995018853A1 (en) * 1994-01-03 1995-07-13 Promega Corporation Mutant luciferases

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8911957D0 (en) 1989-05-24 1989-07-12 Interox Chemicals Ltd Peroxyacid manufacture
US5368484A (en) 1992-05-22 1994-11-29 Atari Games Corp. Vehicle simulator with realistic operating feedback
EP2860252A1 (en) * 1997-09-19 2015-04-15 Promega Corporation Thermostable luciferases and methods of production
JP3694181B2 (ja) * 1999-01-05 2005-09-14 キッコーマン株式会社 変異型ルシフェラーゼ、変異型ルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び変異型ルシフェラーゼの製造法
NZ518413A (en) 1999-10-26 2004-11-26 Secr Defence Novel enzyme
JP4613441B2 (ja) * 2000-04-26 2011-01-19 チッソ株式会社 新規ルシフェラーゼおよび発光蛋白質
EP1156103B1 (en) * 2000-04-26 2010-08-11 Chisso Corporation Oplophorus luciferase
US7118878B1 (en) * 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
US7268229B2 (en) 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
CA2489804C (en) 2002-06-19 2008-03-25 Medical Instill Technologies, Inc. Sterile filling machine having needle filling station within e-beam chamber
US7459527B2 (en) * 2004-03-31 2008-12-02 Xencor, Inc. BMP-7 variants with improved properties
JP3844082B2 (ja) * 2005-04-08 2006-11-08 キッコーマン株式会社 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び耐熱性ホタルルシフェラーゼの製造法
JP4999341B2 (ja) 2005-09-06 2012-08-15 株式会社バイオエネックス 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法
EP2004813B1 (en) * 2006-04-03 2015-06-03 Promega Corporation Permuted and nonpermuted luciferase biosensors binding cyclic nucleotides
WO2008118445A1 (en) * 2007-03-26 2008-10-02 Promega Corporation Methods to quench light from optical reactions
WO2009061413A2 (en) * 2007-11-05 2009-05-14 Promega Corporation Hybrid fusion reporter and uses thereof
EP2281046B1 (en) * 2008-05-19 2012-01-25 Promega Corporation LUCIFERASE BIOSENSORS FOR cAMP
EP2420573A1 (en) * 2009-04-17 2012-02-22 National Institute of Advanced Industrial Science And Technology Stable artificial bioluminescent enzyme having super-high brightness
ES2795287T3 (es) 2009-05-01 2020-11-23 Promega Corp Luciferasas de Oplophorus sintéticas con mayor emisión de luz
SG10202103336SA (en) * 2010-11-02 2021-04-29 Promega Corp Novel coelenterazine substrates and methods of use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995018853A1 (en) * 1994-01-03 1995-07-13 Promega Corporation Mutant luciferases

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output;LOENING ANDREAS MARKUS ET AL;《PROTEIN ENGINEERING DESIGN AND SELECTION》;20061231;第19卷(第9期);391-400 *
Increase in bioluminescence intensity of firefly luciferase using genetic modification;FUJII ET AL;《ANALYTICAL BIOCHEMISTRY》;20071231;第366卷(第2期);131-136 *
Secretional luciferase of the luminous shrimp Oplophorus gracilirostris: cDNA cloning of a novel imidazopyrazinone luciferase;INOUYE S. ET AL;《FEBS LETTERS》;20001231;第481卷(第1期);19-25 *

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