DK3181687T3 - Synthetic oplophorus luciferases with increased light yield - Google Patents
Synthetic oplophorus luciferases with increased light yield Download PDFInfo
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- DK3181687T3 DK3181687T3 DK17154777.1T DK17154777T DK3181687T3 DK 3181687 T3 DK3181687 T3 DK 3181687T3 DK 17154777 T DK17154777 T DK 17154777T DK 3181687 T3 DK3181687 T3 DK 3181687T3
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17483809P | 2009-05-01 | 2009-05-01 | |
| EP15186652.2A EP2990478B1 (en) | 2009-05-01 | 2010-05-03 | Synthetic oplophorus luciferases with enhanced light output |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3181687T3 true DK3181687T3 (en) | 2018-09-10 |
Family
ID=42358981
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17154777.1T DK3181687T3 (en) | 2009-05-01 | 2010-05-03 | Synthetic oplophorus luciferases with increased light yield |
| DK18184699.9T DK3409764T3 (da) | 2009-05-01 | 2010-05-03 | Syntetiske oplophorus-luciferaser med forbedret lysudbytte |
| DK10717013.6T DK2456864T3 (en) | 2009-05-01 | 2010-05-03 | SYNTHETIC OPLOPHORUS luciferases WITH IMPROVED LIGHT MISSION |
| DK15186652.2T DK2990478T3 (en) | 2009-05-01 | 2010-05-03 | SYNTHETIC OPLOPHORUS LUCIFERASES WITH IMPROVED LIGHT EMISSION |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK18184699.9T DK3409764T3 (da) | 2009-05-01 | 2010-05-03 | Syntetiske oplophorus-luciferaser med forbedret lysudbytte |
| DK10717013.6T DK2456864T3 (en) | 2009-05-01 | 2010-05-03 | SYNTHETIC OPLOPHORUS luciferases WITH IMPROVED LIGHT MISSION |
| DK15186652.2T DK2990478T3 (en) | 2009-05-01 | 2010-05-03 | SYNTHETIC OPLOPHORUS LUCIFERASES WITH IMPROVED LIGHT EMISSION |
Country Status (16)
| Country | Link |
|---|---|
| US (7) | US8557970B2 (https=) |
| EP (5) | EP2990478B1 (https=) |
| JP (2) | JP6038649B2 (https=) |
| KR (1) | KR101665352B1 (https=) |
| CN (2) | CN102459579B (https=) |
| AU (1) | AU2010242771B2 (https=) |
| BR (1) | BRPI1010019B1 (https=) |
| CA (1) | CA2758572A1 (https=) |
| DK (4) | DK3181687T3 (https=) |
| ES (5) | ES2795287T3 (https=) |
| IL (3) | IL215697B (https=) |
| LT (1) | LT3409764T (https=) |
| PL (5) | PL2456864T3 (https=) |
| PT (4) | PT3181687T (https=) |
| SG (3) | SG10201901453PA (https=) |
| WO (1) | WO2010127368A1 (https=) |
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| WO2013126584A1 (en) | 2012-02-21 | 2013-08-29 | Laboratory Corporation Of America Holdings | Methods and systems for detection of microorganisms |
| US10519483B2 (en) | 2012-02-21 | 2019-12-31 | Laboratory Corporation Of America Holdings | Methods and systems for rapid detection of microorganisms using infectious agents |
| US20140099654A1 (en) | 2012-09-26 | 2014-04-10 | Promega Corporation | Real-time monitoring |
| AU2013359160B2 (en) | 2012-12-12 | 2018-11-15 | Promega Corporation | Compositions and methods for capture of cellular targets of bioactive agents |
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| WO2010119721A1 (ja) | 2009-04-17 | 2010-10-21 | 独立行政法人産業技術総合研究所 | 超高輝度で安定な人工生物発光酵素 |
| LT3409764T (lt) | 2009-05-01 | 2020-06-10 | Promega Corporation | Sintetinės oplophorus liuciferazės su sustiprinta šviesos išeiga |
| BR112013010487B1 (pt) * | 2010-11-02 | 2021-02-02 | Promega Corporation | compostos de coelenterazina, kit compreendendo ditos compostos e método para detectar luminescência em uma amostra in vitro |
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