CA2758572A1 - Synthetic oplophorus luciferases with enhanced light output - Google Patents
Synthetic oplophorus luciferases with enhanced light output Download PDFInfo
- Publication number
- CA2758572A1 CA2758572A1 CA2758572A CA2758572A CA2758572A1 CA 2758572 A1 CA2758572 A1 CA 2758572A1 CA 2758572 A CA2758572 A CA 2758572A CA 2758572 A CA2758572 A CA 2758572A CA 2758572 A1 CA2758572 A1 CA 2758572A1
- Authority
- CA
- Canada
- Prior art keywords
- polynucleotide
- luciferase
- amino acid
- ogluc
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17483809P | 2009-05-01 | 2009-05-01 | |
| US61/174,838 | 2009-05-01 | ||
| PCT/US2010/033449 WO2010127368A1 (en) | 2009-05-01 | 2010-05-03 | Synthetic oplophorus luciferases with enhanced light output |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2758572A1 true CA2758572A1 (en) | 2010-11-04 |
Family
ID=42358981
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2758572A Pending CA2758572A1 (en) | 2009-05-01 | 2010-05-03 | Synthetic oplophorus luciferases with enhanced light output |
Country Status (16)
| Country | Link |
|---|---|
| US (7) | US8557970B2 (https=) |
| EP (5) | EP2990478B1 (https=) |
| JP (2) | JP6038649B2 (https=) |
| KR (1) | KR101665352B1 (https=) |
| CN (2) | CN102459579B (https=) |
| AU (1) | AU2010242771B2 (https=) |
| BR (1) | BRPI1010019B1 (https=) |
| CA (1) | CA2758572A1 (https=) |
| DK (4) | DK3181687T3 (https=) |
| ES (5) | ES2795287T3 (https=) |
| IL (3) | IL215697B (https=) |
| LT (1) | LT3409764T (https=) |
| PL (5) | PL2456864T3 (https=) |
| PT (4) | PT3181687T (https=) |
| SG (3) | SG10201901453PA (https=) |
| WO (1) | WO2010127368A1 (https=) |
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| LT3409764T (lt) | 2009-05-01 | 2020-06-10 | Promega Corporation | Sintetinės oplophorus liuciferazės su sustiprinta šviesos išeiga |
| BR112013010487B1 (pt) | 2010-11-02 | 2021-02-02 | Promega Corporation | compostos de coelenterazina, kit compreendendo ditos compostos e método para detectar luminescência em uma amostra in vitro |
| WO2012061477A1 (en) | 2010-11-02 | 2012-05-10 | Promega Corporation | Coelenterazine derivatives and methods of using same |
| JP5896679B2 (ja) * | 2011-03-15 | 2016-03-30 | オリンパス株式会社 | オオオバボタル由来ルシフェラーゼ |
| EP2782916B1 (en) | 2011-11-21 | 2018-02-28 | Promega Corporation | Carboxy x rhodamine analogs |
| WO2013126584A1 (en) | 2012-02-21 | 2013-08-29 | Laboratory Corporation Of America Holdings | Methods and systems for detection of microorganisms |
| US10519483B2 (en) | 2012-02-21 | 2019-12-31 | Laboratory Corporation Of America Holdings | Methods and systems for rapid detection of microorganisms using infectious agents |
| US20140099654A1 (en) | 2012-09-26 | 2014-04-10 | Promega Corporation | Real-time monitoring |
| AU2013359160B2 (en) | 2012-12-12 | 2018-11-15 | Promega Corporation | Compositions and methods for capture of cellular targets of bioactive agents |
| US10067149B2 (en) | 2012-12-12 | 2018-09-04 | Promega Corporation | Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer |
| AU2013359166B2 (en) | 2012-12-12 | 2019-09-19 | Promega Corporation | Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer |
| JP6511720B2 (ja) | 2013-02-28 | 2019-05-15 | Jnc株式会社 | コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法 |
| EP2969435B1 (en) | 2013-03-15 | 2021-11-03 | Promega Corporation | Substrates for covalent tethering of proteins to functional groups or solid surfaces |
| US10168323B2 (en) | 2013-03-15 | 2019-01-01 | Promega Corporation | Compositions and methods for capture of cellular targets of bioactive agents |
| SG11201507306VA (en) | 2013-03-15 | 2015-10-29 | Promega Corp | Activation of bioluminescence by structural complementation |
| JP2016517687A (ja) | 2013-03-27 | 2016-06-20 | サンプル テクノロジーズ，インコーポレイティド | 組換えファージ及び細菌検出方法 |
| JP6040846B2 (ja) | 2013-04-08 | 2016-12-07 | Jnc株式会社 | セレンテラジン類縁体 |
| AU2014281406A1 (en) | 2013-06-19 | 2016-01-21 | Sample6 Technologies, Inc. | Phage-based bacterial detection assay |
| JP6484987B2 (ja) | 2013-10-21 | 2019-03-20 | Jnc株式会社 | エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法 |
| JP6442825B2 (ja) | 2013-12-26 | 2018-12-26 | Jnc株式会社 | エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法 |
| US9927430B2 (en) | 2014-01-29 | 2018-03-27 | Promega Corporation | Pro-substrates for live cell applications |
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| JP6256118B2 (ja) | 2014-03-11 | 2018-01-10 | Jnc株式会社 | エビルシフェラーゼの触媒蛋白質のキメラ遺伝子及びその使用法 |
| JP6265020B2 (ja) | 2014-04-16 | 2018-01-24 | Jnc株式会社 | エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法 |
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| EP3254113B1 (en) | 2015-02-05 | 2024-10-09 | Promega Corporation | Luciferase-based thermal shift assays |
| JP6876002B2 (ja) | 2015-06-05 | 2021-05-26 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 機能的要素を共有結合により係留させるための細胞透過性、細胞適合性、かつ開裂可能であるリンカー |
| CN107922428B (zh) | 2015-06-25 | 2021-02-05 | 普洛麦格公司 | 噻吩并吡咯化合物及其作为刺虾源性荧光素酶的抑制剂的用途 |
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| EP4596707A3 (en) | 2016-09-19 | 2025-10-08 | University of Southern California | Non-radioactive cytotoxicity assays |
| WO2018075783A2 (en) | 2016-10-20 | 2018-04-26 | President And Fellows Of Harvard College | In vitro and cell based assays for measuring the activity of botulinum neurotoxins |
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| EP3395803A1 (en) | 2017-04-28 | 2018-10-31 | Institut Pasteur | Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins |
| WO2019081620A1 (en) * | 2017-10-25 | 2019-05-02 | Roche Diagnostics Gmbh | IMPROVED MODIFIED / MUTANT BACTERIAL LUCIFERASES |
| US20200393446A1 (en) | 2017-12-08 | 2020-12-17 | Centre National De La Recherche Scientifique (Cnrs) | Bioluminescent screening test for detecting cell cytolysis |
| CN111936623B (zh) * | 2018-03-30 | 2023-08-25 | 英威达纺织（英国）有限公司 | 用于生物合成制造庚二酸的材料与方法和合成多肽的利用 |
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| KR20230124938A (ko) | 2020-12-25 | 2023-08-28 | 추가이 세이야쿠 가부시키가이샤 | 복수의 표적 분자와 함께 복합체를 형성할 수 있는후보 분자의 스크리닝 방법 |
| CN115161296B (zh) * | 2021-04-01 | 2024-08-13 | 深圳华大生命科学研究院 | 一种刺虾荧光素酶Nluc的突变体及其应用 |
| CN113025586B (zh) * | 2021-04-06 | 2022-10-04 | 湖北省中医院 | 一种经改造的萤光素酶突变体蛋白、生物发光探针、探针组、制备方法和检测方法 |
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| CN119307519B (zh) * | 2024-07-22 | 2025-11-25 | 苏州剂泰医药科技有限公司 | 编码荧光素酶的mRNA及其应用 |
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| BR112013010487B1 (pt) * | 2010-11-02 | 2021-02-02 | Promega Corporation | compostos de coelenterazina, kit compreendendo ditos compostos e método para detectar luminescência em uma amostra in vitro |
-
2010
- 2010-05-03 LT LTEP18184699.9T patent/LT3409764T/lt unknown
- 2010-05-03 ES ES18184699T patent/ES2795287T3/es active Active
- 2010-05-03 JP JP2012508822A patent/JP6038649B2/ja active Active
- 2010-05-03 EP EP15186652.2A patent/EP2990478B1/en active Active
- 2010-05-03 EP EP20169057.5A patent/EP3744834B1/en active Active
- 2010-05-03 PL PL10717013T patent/PL2456864T3/pl unknown
- 2010-05-03 SG SG10201901453PA patent/SG10201901453PA/en unknown
- 2010-05-03 EP EP17154777.1A patent/EP3181687B1/en active Active
- 2010-05-03 DK DK17154777.1T patent/DK3181687T3/en active
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