CN101988082B - 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法 - Google Patents

白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法 Download PDF

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Publication number
CN101988082B
CN101988082B CN200910109215.6A CN200910109215A CN101988082B CN 101988082 B CN101988082 B CN 101988082B CN 200910109215 A CN200910109215 A CN 200910109215A CN 101988082 B CN101988082 B CN 101988082B
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leukocyte
reagent
classified counting
compound
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CN101988082A (zh
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赵阳
雷霆
赵玉梅
徐兵
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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Priority to US12/843,671 priority patent/US8685661B2/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1468Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle
    • G01N15/147Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966Animal cells
    • G01N33/56972White blood cells
    • G01N2015/016
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1477Multiparameters
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1486Counting the particles
CN200910109215.6A 2009-07-31 2009-07-31 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法 Active CN101988082B (zh)

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CN200910109215.6A CN101988082B (zh) 2009-07-31 2009-07-31 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法
US12/843,671 US8685661B2 (en) 2009-07-31 2010-07-26 Reagent and kit for classifying and counting leukocytes, the preparation thereof, and process for classifying and counting leukocytes

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CN200910109215.6A CN101988082B (zh) 2009-07-31 2009-07-31 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法

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Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5865009B2 (ja) * 2011-10-25 2016-02-17 シスメックス株式会社 活性化好中球の検出方法
JP6208473B2 (ja) 2012-06-20 2017-10-04 アークレイ株式会社 血液成分を含む試料の処理方法
JP6001425B2 (ja) * 2012-11-26 2016-10-05 シスメックス株式会社 血球分析方法、血球分析装置およびプログラム
CN103217322A (zh) * 2013-03-20 2013-07-24 江苏美诚生物科技有限公司 适用于白细胞分类的试剂及方法
CN104749144A (zh) * 2013-12-31 2015-07-01 深圳迈瑞生物医疗电子股份有限公司 血细胞检测试剂及血细胞处理方法和识别方法
JP6092132B2 (ja) * 2014-01-29 2017-03-08 シスメックス株式会社 血球分析装置
CN106687810B (zh) * 2014-12-31 2019-10-22 深圳迈瑞生物医疗电子股份有限公司 一种非诊断目的的有核红细胞报警方法、装置及流式细胞分析仪
CN105986003B (zh) * 2015-02-12 2020-11-13 深圳迈瑞生物医疗电子股份有限公司 一种白细胞计数方法、装置及细胞分析仪
CN108318408B (zh) * 2018-01-30 2020-04-17 深圳唯公生物科技有限公司 白细胞分类用样本的预处理试剂及方法
WO2019206300A1 (zh) * 2018-04-28 2019-10-31 深圳迈瑞生物医疗电子股份有限公司 一种血液检测方法及血液分析系统
CN111656188B (zh) * 2018-04-28 2021-10-12 深圳迈瑞生物医疗电子股份有限公司 试剂、分析血小板的方法及血液细胞分析仪
CN109580550A (zh) * 2018-12-03 2019-04-05 迪瑞医疗科技股份有限公司 一种白细胞的分类处理方法及其装置
CN110031383A (zh) * 2019-04-22 2019-07-19 深圳开立生物医疗科技股份有限公司 一种白细胞分类试剂及方法
CN110542640A (zh) * 2019-08-12 2019-12-06 江苏美诚生物科技有限公司 一种五分类血细胞分析仪用白细胞分类试剂盒
CN110579595B (zh) * 2019-09-10 2022-02-18 核工业总医院 一种等张溶血素及其制备方法、处理生物样本方法和检测白细胞膜抗原方法
CN111088313A (zh) * 2019-12-16 2020-05-01 中国水产科学研究院珠江水产研究所 一种用于快速固定嗜热四膜虫细胞计数的试剂及其计数方法
CN111006990A (zh) * 2019-12-31 2020-04-14 山东博科生物产业有限公司 血细胞分析用试剂及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101343420A (zh) * 2007-07-12 2009-01-14 大连理工大学 一类不对称菁类荧光染料
CN101349644A (zh) * 2007-07-20 2009-01-21 深圳迈瑞生物医疗电子股份有限公司 一种白细胞分类试剂和其使用方法

Family Cites Families (130)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2114067A5 (US20110027788A1-20110203-C00014.png) * 1970-11-13 1972-06-30 Sigma
US4146604A (en) * 1973-05-31 1979-03-27 Block Engineering, Inc. Differential counting of leukocytes and other cells
IL49622A0 (en) * 1976-05-21 1976-07-30 Elscint Ltd A method and apparatus for classifying biological cells
US4286963A (en) * 1979-11-23 1981-09-01 Coulter Electronics, Inc. Differential lymphoid-myeloid determination of leukocytes in whole blood
US4332785A (en) * 1980-04-09 1982-06-01 University Patents, Inc. Immunoassay for measurement of reticulocytes, and immunoreactive reagents for use therein
US4447547A (en) * 1980-04-09 1984-05-08 University Patents, Inc. Immunoassay for measurement of reticulocytes, and immunoreactive reagents for use therein
US4544546A (en) 1980-04-21 1985-10-01 Abbott Laboratories Fluorescent nucleic acid stains
US4336029A (en) * 1980-08-15 1982-06-22 Ortho Diagnostic Systems Inc. Method and reagents for quantitative determination of reticulocytes and platelets in whole blood
US4325706A (en) * 1980-08-15 1982-04-20 Ortho Diagnostic Systems Inc. Automated detection of platelets and reticulocytes in whole blood
JPS5747493A (en) 1980-09-02 1982-03-18 Fuji Photo Film Co Ltd Method of determining a trace amount of enzyme
US4528274A (en) * 1982-07-06 1985-07-09 Coulter Electronics, Inc. Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
US4485175A (en) 1983-01-03 1984-11-27 Coulter Electronics, Inc. Method for three-volume differential determination of lymphocyte, monocyte and granulocyte populations of leukocytes
US4571388A (en) * 1983-01-24 1986-02-18 Becton Dickinson And Company Detection of reticulocytes
US4529705A (en) * 1983-06-06 1985-07-16 Coulter Electronics, Inc. Reagent for combined diluting and lysing whole blood
US4596035A (en) * 1983-06-27 1986-06-17 Ortho Diagnostic Systems Inc. Methods for enumerating 3-part white cell differential clusters
US4637986A (en) * 1983-08-22 1987-01-20 Ortho Diagnostic Systems, Inc. Flow cytometry lysing reagent with leukoprotective agent for producing a 3-part WBC count
JPS6073356A (ja) 1983-09-29 1985-04-25 Toa Medical Electronics Co Ltd 血液分析用試薬
US4751179A (en) * 1984-05-31 1988-06-14 Coulter Electronics, Inc. Method and reagents for differential determination of four populations of leukocytes in blood
US5188935A (en) * 1984-05-31 1993-02-23 Coulter Electronics, Inc. Reagent system and method for identification, enumeration and examination of classes and subclasses of blood leukocytes
CA1255197A (en) * 1984-09-24 1989-06-06 Joseph L. Orlik Leukocyte differentiation composition and method
JPS61280565A (ja) * 1985-06-06 1986-12-11 Toa Medical Electronics Co Ltd フロ−サイトメトリ−用網状血球測定試薬
US4745071A (en) * 1985-09-05 1988-05-17 Sequoia-Turner Corporation Method for the volumetric differentiation of blood cells types
US4978624A (en) 1985-09-06 1990-12-18 Technicon Instruments Corporation Reagent for the determination of a differential white blood cell count
US4707451A (en) 1985-09-18 1987-11-17 Becton, Dickinson And Company Detection of reticulocytes
US4957870A (en) * 1985-11-01 1990-09-18 Becton, Dickinson And Company Detection of Reticulocytes, RNA and DNA
US4883867A (en) 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
US5175109A (en) 1986-09-10 1992-12-29 Toa Medical Electronics Co., Ltd. Reagent for classifying leukocytes by flow cytometry
JPH06100596B2 (ja) * 1986-09-10 1994-12-12 東亜医用電子株式会社 フロ−サイトメトリ−による白血球の分類方法
US5039613A (en) * 1986-11-27 1991-08-13 Toa Medical Electronics Co., Ltd. Reagents used in a method of classifying leukocytes by flow cytometry
US5179026A (en) * 1986-11-27 1993-01-12 Toa Medical Electronics Co., Ltd. Method of classifying leukocytes by flow cytometry and reagents used in the method
US4822745A (en) * 1986-12-05 1989-04-18 Albert Einstein College Of Medicine Of Yeshiva University Method for quantifying human reticulocytes
IL85532A (en) * 1987-03-13 1992-03-29 Coulter Electronics Method and lytic reagent system for isolation,identification and/or analysis of leukocytes from whole blood samples
US5155044A (en) 1987-03-13 1992-10-13 Coulter Electronics, Inc. Lysing reagent system for isolation, identification and/or analysis of leukocytes from whole blood samples
US4882284A (en) 1987-04-13 1989-11-21 Ortho Pharmaceutical Corporation Method for quantitating and differentiating white blood cells
US5389549A (en) * 1987-05-29 1995-02-14 Toa Medical Electronics Co., Ltd. Method for classifying leukocytes and a reagent used therefor
JPH0746102B2 (ja) 1987-07-31 1995-05-17 東亜医用電子株式会社 フロ−サイトメトリ用網状赤血球測定試薬
JP2549665B2 (ja) * 1987-07-31 1996-10-30 東亜医用電子株式会社 フロ−サイトメトリ用網状赤血球測定試薬
JP2619900B2 (ja) * 1988-01-27 1997-06-11 東亜医用電子株式会社 血液中の白血球およびヘモグロビンの測定用試薬および方法
US5180677A (en) * 1988-07-05 1993-01-19 Fisher Scientific Company Lysing reagent for leukocyte differentiation method
CA2016699C (en) * 1989-05-15 2003-11-18 Paul N. Marshall Lytic agents and uses thereof
US6248319B1 (en) * 1989-10-16 2001-06-19 Krisztina M. Zsebo Method for increasing hematopoietic progenitor cells by stem cell factor polypeptides
JP2836865B2 (ja) 1989-10-23 1998-12-14 東亜医用電子株式会社 血液中の白血球およびヘモグロビンの測定用試薬
FR2654744B1 (fr) * 1989-11-20 1992-03-13 Abx Sa Reactif et methode d'utilisation de celui-ci pour la determination automatique en cytometrie de flux d'au moins une sous-population leucocytaire a partir du sang total.
US5075556A (en) 1989-12-01 1991-12-24 Technicon Instruments Corporation Acridine orange derivatives and their use in the quantitation of reticulocytes in whole blood
US5242832A (en) * 1990-03-01 1993-09-07 Toa Medical Electronics Co., Ltd. Reagent for measurement of leukocytes and hemoglobin in blood
DE4033355C2 (de) * 1990-10-19 1999-08-26 Siemens Ag Verfahren zum elektrolytischen Ätzen von Siliziumcarbid
US5227304A (en) 1991-01-16 1993-07-13 Sequoia Turner Corporation Method for counting whole blood diluent and detergent reagent system
US5316951A (en) * 1991-06-07 1994-05-31 Carver Jr Edward L Method for the improved determination of white blood cell subpopulations
US5316725A (en) * 1991-06-07 1994-05-31 Edward Lawrence Carver, Jr. Reagent system for the improved determination of white blood cell subpopulations
US5262329A (en) * 1991-06-13 1993-11-16 Carver Jr Edward L Method for improved multiple species blood analysis
JP3048260B2 (ja) 1991-07-29 2000-06-05 シスメックス株式会社 白血球分類計数用試料調製方法
US5284771A (en) * 1991-12-05 1994-02-08 Miles Inc. Reagent compositions and their use in sphering cells
US5360739A (en) 1991-12-05 1994-11-01 Miles Inc. Methods for the identification and characterization of reticulocytes in whole blood
US5350695A (en) * 1991-12-05 1994-09-27 Miles Inc. Methods for the identification and characterization of reticulocytes in whole blood
JP3232145B2 (ja) 1991-12-27 2001-11-26 シスメックス株式会社 網赤血球測定方法
CA2087086A1 (en) * 1992-01-22 1993-07-23 Leon Wmm Terstappen Multidimensional cell differential analysis
US5321130A (en) * 1992-02-10 1994-06-14 Molecular Probes, Inc. Unsymmetrical cyanine dyes with a cationic side chain
DE69327775T2 (de) * 1992-11-19 2000-06-21 Sysmex Corp Verfahren zur Vorbehandlung für Blutanalyse
DE69434942T2 (de) * 1993-02-25 2007-11-29 Abbott Laboratories, Abbott Park Mehrzweckreagenzsystem zur schnellen lysierung von vollblutproben
JP3301646B2 (ja) * 1993-03-19 2002-07-15 シスメックス株式会社 幼若細胞測定用試薬
AU6830894A (en) * 1993-05-14 1994-12-12 Coulter Corporation Reticulocyte analyzing method and apparatus utilizing light scatter techniques
US5563070A (en) 1993-05-28 1996-10-08 Omron Corporation Method of counting reticulocytes
JP3320869B2 (ja) * 1993-12-22 2002-09-03 シスメックス株式会社 白血球分析用試薬
JP3467310B2 (ja) * 1994-04-21 2003-11-17 シスメックス株式会社 白血球分析用試薬及び白血球の分類方法
IL113805A0 (en) * 1994-05-23 1995-08-31 Coulter Corp Detection of reticulocytes
JP3355038B2 (ja) 1994-08-03 2002-12-09 シスメックス株式会社 白血球分類方法
JP3324050B2 (ja) * 1994-10-31 2002-09-17 日本光電工業株式会社 白血球分類用試薬および白血球分類方法
US5879900A (en) * 1994-12-15 1999-03-09 Abbott Laboratories Method for simultaneous analysis of cell viability, nucleated red blood cells and white blood cell differentials
US5559037A (en) * 1994-12-15 1996-09-24 Abbott Laboratories Method for rapid and simultaneous analysis of nucleated red blood cells
US5656449A (en) * 1995-03-06 1997-08-12 Molecular Probes, Inc. Neutral unsymmetrical cyanine dyes
US5691204A (en) 1995-04-21 1997-11-25 Abbott Laboratories Compositions and methods for the rapid analysis of reticulocytes
US5639630A (en) * 1995-05-16 1997-06-17 Bayer Corporation Method and reagent composition for performing leukocyte differential counts on fresh and aged whole blood samples, based on intrinsic peroxidase activity of leukocytes
US5843608A (en) 1995-06-08 1998-12-01 Coulter International Corp. Reagent and method for differential determination of leukocytes in blood
US5686308A (en) 1995-06-08 1997-11-11 Coulter Corporation Reagent and method for differential determination of leukocytes in blood
FR2735579B1 (fr) 1995-06-13 1997-09-19 Hycel Groupe Lisabio Procede de discrimination et d'isolement de sous-populations de leucocytes a partir d'echantillons sanguins par traitement par du polyoxyethylene 9-lauryl ether, et reactif pour sa mise en oeuvre
FR2735578B1 (fr) 1995-06-13 1997-09-05 Hycel Groupe Lisabio Reactif de lyse des erythrocytes, et son utilisation dans des procedes d'isolement et de discrimination des leucocytes
US5786224A (en) * 1995-06-29 1998-07-28 Coulter Corporation Reagent and method for differential determination of leukocytes in blood
JP3425830B2 (ja) 1995-10-06 2003-07-14 シスメックス株式会社 新規化合物とその用途
US5658735A (en) * 1995-11-09 1997-08-19 Biometric Imaging, Inc. Cyclized fluorescent nucleic acid intercalating cyanine dyes and nucleic acid detection methods
US5733784A (en) * 1995-11-20 1998-03-31 Abbott Laboratories Reagent system and method for the differentiation and identification of reticulocytes
US5817518A (en) 1995-12-18 1998-10-06 Coulter International Corp. Reagent and method for differential determination of leukocytes in blood
DE69625581T2 (de) 1996-03-07 2003-09-25 Coulter Int Corp Cyanidfreies Reagenz und Verfahren zur Bestimmung von Hämoglobin und Leukozytendifferenzierung
TW379284B (en) * 1996-04-12 2000-01-11 Toa Medical Electronics Agent for detecting reticulocyte
CN1149397C (zh) 1996-04-12 2004-05-12 希森美康株式会社 网织红细胞测定用试剂
US6245499B1 (en) * 1996-04-30 2001-06-12 Fuji Photo Film Co., Ltd. Photothermographic material
US5858667A (en) * 1996-09-06 1999-01-12 Litron Laboratories Method for the enumeration of micronucleated erythrocyte populations with a single laser flow cytometer
US6100038A (en) * 1996-09-06 2000-08-08 Litron Laboratories Limited Method for the enumeration of micronucleated erythrocyte populations with a single laser flow cytometer
JP4042925B2 (ja) * 1996-11-20 2008-02-06 シスメックス株式会社 幼若白血球の分類計数法
US6495692B1 (en) * 1996-12-10 2002-12-17 Abbott Laboratories Helium-neon excitable reticulocyte dyes derivable from halolepidines
US5763280A (en) * 1997-01-21 1998-06-09 Coulter International Corp. Cyanide-free lytic reagent composition and method for hemoglobin and cell analysis
US5882934A (en) * 1997-01-21 1999-03-16 Coulter International Corp. Composition and method for hemoglobin and cell analysis
FR2759166B1 (fr) 1997-01-31 1999-04-23 Abx Sa Reactif de coloration pour la determination de cellules sanguines
US6114173A (en) * 1997-04-03 2000-09-05 Bayer Corporation Fully automated method and reagent composition therefor for rapid identification and characterization of reticulocytes erythrocytes and platelets in whole blood
JP3830613B2 (ja) * 1997-04-18 2006-10-04 シスメックス株式会社 血液中の白血球及びヘモグロビン濃度測定試薬
US5994089A (en) 1997-05-16 1999-11-30 Coulter International Corp. Simultaneous analyses of white blood cell subsets using multi-color, multi-intensity fluorescent markers in flow cytometry
JP3783808B2 (ja) 1997-05-19 2006-06-07 シスメックス株式会社 白血球分類計数用試薬
US5874311A (en) * 1997-11-21 1999-02-23 Coulter International Corp. Method for differentiation of reticulocytes in blood
US6350613B1 (en) * 1998-03-07 2002-02-26 Belton Dickinson & Co. Determination of white blood cell differential and reticulocyte counts
US6074232A (en) 1998-06-01 2000-06-13 Hon Hai Precision Ind. Co., Ltd. Board to board electrical connector with releaseable actuator
FR2782166B1 (fr) * 1998-08-04 2000-10-06 Abx Sa Reactif pour la mesure de l'hemoglobine et la determination des leucocytes dans un echantillon de sang
US6197593B1 (en) * 1998-10-20 2001-03-06 Coulter International Corp. Method for enumerating blood cells
US6271035B1 (en) * 1998-10-20 2001-08-07 Coulter International Corp. Methods and compositions for rapid staining of nucleic acids in whole cells
US6060322A (en) * 1998-10-20 2000-05-09 Coulter International Corp. Method for identification of reticulated cells
JP3886271B2 (ja) 1998-11-27 2007-02-28 シスメックス株式会社 赤芽球の分類計数用試薬及び分類計数方法
BR0009420A (pt) * 1999-03-31 2002-04-09 Bayer Ag Método de detecção de diluição simples e canal único
US6900023B1 (en) * 1999-09-02 2005-05-31 Sysmex Corporation Method for classifying and counting leukocytes
US20020098589A1 (en) 2001-01-19 2002-07-25 Crews Harold Richardson Multi-purpose reagent system and method for enumeration of red blood cells, white blood cells and thrombocytes and differential determination of white blood cells
US6632676B1 (en) 1999-09-24 2003-10-14 Clinical Diagnostic Solutions Multi-purpose reagent system and method for enumeration of red blood cells, white blood cells and thrombocytes and differential determination of white blood cells
CN1101982C (zh) 1999-10-29 2003-02-19 富士康(昆山)电脑接插件有限公司 插座连接器及其导电端子
US6368864B1 (en) * 2000-05-05 2002-04-09 Coulter International Corp. Dyes and methods of reticulocyte enumeration
JP3493575B2 (ja) 2000-07-28 2004-02-03 Necエレクトロニクス株式会社 半導体装置、チャージポンプ回路およびpll回路
FR2813891B1 (fr) * 2000-09-14 2005-01-14 Immunotech Sa Reactif multifonctionnel pour erythrocytes mettant en jeu des carbamates et applications
US6794152B2 (en) * 2000-12-22 2004-09-21 Streck Laboratories Inc. Flow cytometry reagent and system
FR2821428B1 (fr) * 2001-02-23 2004-08-06 Abx Sa Reactif et procede pour l'identification et le comptage de cellules biologiques
US6630990B2 (en) 2001-06-05 2003-10-07 Abbott Laboratories Optical method and apparatus for red blood cell differentiation on a cell-by-cell basis, and simultaneous analysis of white blood cell differentiation
AU2003232168A1 (en) * 2002-06-11 2003-12-22 Chempaq A/S Lysing reagent, cartridge and automatic electronic cell counter for simultaneous enumeration of different types of white blood cells
AU2003243945A1 (en) * 2002-06-24 2004-01-06 Sysmex Corporation Method of classifying and counting leucocytes
US7405082B2 (en) * 2002-09-10 2008-07-29 Sysmex Corporation Methods and devices for measuring reticulocytes
EP1422509A1 (en) * 2002-11-01 2004-05-26 Streck Laboratories, Inc. Lysing Reagent and Method of Lysing Red Blood Cells for Hematology
US6955872B2 (en) 2003-03-20 2005-10-18 Coulter International Corp. Dye compositions which provide enhanced differential fluorescence and light scatter characteristics
US6869798B2 (en) * 2003-04-17 2005-03-22 Clinical Diagnostics Solutions, Inc. Lytic reagent composition for leukocyte differential analysis
JP4509607B2 (ja) 2004-03-17 2010-07-21 シスメックス株式会社 細胞分析装置および方法
US7465584B2 (en) 2005-01-24 2008-12-16 Sysmex Corporation Method and reagent for classifying leukocytes in animal blood
US7235404B2 (en) * 2005-05-04 2007-06-26 Beckman Coulter, Inc. Cyanide-free lytic reagent composition and method of use for hemoglobin and white blood cell measurement
US7625757B2 (en) * 2006-01-27 2009-12-01 Sysmex Corporation Reagent for immature leukocyte analysis and reagent kit
JP4806334B2 (ja) 2006-11-27 2011-11-02 シスメックス株式会社 血液学的試料の測定方法および測定装置
US7449337B2 (en) 2007-01-24 2008-11-11 Idexx Laboratories, Inc. Lytic reagent and method for leukocytes differential in whole blood
CN105440725A (zh) * 2008-01-04 2016-03-30 深圳迈瑞生物医疗电子股份有限公司 不对称菁类荧光染料,组合物及在生物样品染色中的用途
CN101602762B (zh) * 2008-06-10 2013-10-16 深圳迈瑞生物医疗电子股份有限公司 不对称菁类化合物、其制备方法及应用
US8367358B2 (en) * 2008-12-17 2013-02-05 Shenzhen Mindray Bio-Medical Electronics Co., Ltd. Reagent, kit and method for differentiating and counting leukocytes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101343420A (zh) * 2007-07-12 2009-01-14 大连理工大学 一类不对称菁类荧光染料
CN101349644A (zh) * 2007-07-20 2009-01-21 深圳迈瑞生物医疗电子股份有限公司 一种白细胞分类试剂和其使用方法

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