CN101023181A - 提高类异戊二烯化合物的产生的方法 - Google Patents

提高类异戊二烯化合物的产生的方法 Download PDF

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Publication number
CN101023181A
CN101023181A CNA2005800243430A CN200580024343A CN101023181A CN 101023181 A CN101023181 A CN 101023181A CN A2005800243430 A CNA2005800243430 A CN A2005800243430A CN 200580024343 A CN200580024343 A CN 200580024343A CN 101023181 A CN101023181 A CN 101023181A
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coa
host cell
hmg
cell
genetically modified
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Chinese (zh)
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J·D·基斯林
J·D·纽曼
D·J·皮特拉
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University of California San Diego UCSD
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen

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  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Steroid Compounds (AREA)
CNA2005800243430A 2004-05-21 2005-05-20 提高类异戊二烯化合物的产生的方法 Pending CN101023181A (zh)

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US57349204P 2004-05-21 2004-05-21
US60/573,492 2004-05-21

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CN101023181A true CN101023181A (zh) 2007-08-22

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US (2) US7183089B2 (https=)
EP (2) EP2365090A1 (https=)
JP (1) JP4926061B2 (https=)
CN (1) CN101023181A (https=)
AT (1) ATE537269T1 (https=)
AU (1) AU2005327292B2 (https=)
BR (1) BRPI0510115B1 (https=)
CA (1) CA2567547C (https=)
ES (1) ES2379368T3 (https=)
MX (1) MXPA06013502A (https=)
WO (1) WO2006085899A2 (https=)

Cited By (9)

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CN103602626A (zh) * 2013-10-12 2014-02-26 中国科学院青岛生物能源与过程研究所 一种以油脂为原料生产甲羟戊酸的方法及其构建的基因工程菌
CN104039974A (zh) * 2011-11-09 2014-09-10 阿迈瑞斯公司 乙酰辅酶a衍生的类异戊二烯的生产
CN105112454A (zh) * 2008-05-16 2015-12-02 Reg生命科学有限责任公司 产生碳氢化合物的方法和组合物
CN106119112A (zh) * 2008-03-27 2016-11-16 基因组股份公司 用于产生己二酸和其他化合物的微生物
CN106367508A (zh) * 2016-09-13 2017-02-01 东北农业大学 一种用于鉴定杂合型类异戊二烯化合物产生菌的简并引物与应用
US9816124B2 (en) 2013-02-01 2017-11-14 Tianjin Institute Of Industrial Biotechnology, Chinese Academy Of Sciences Recombinant microorganism for preparing terpenoid and method for constructing recombinant microorganism
CN116925991A (zh) * 2023-07-28 2023-10-24 天津大学 高产甲羟戊酸的重组盐单胞菌菌株及构建方法与应用
WO2023227104A1 (zh) * 2022-05-26 2023-11-30 牛津大学(苏州)科技有限公司 一种用于合成萜烯的共培养系统和方法
WO2025108471A1 (zh) * 2023-11-24 2025-05-30 牛津大学(苏州)科技有限公司 用于合成目标化合物的共培养系统和方法

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE286063T1 (de) * 1996-04-17 2005-01-15 Hubert Dr Koester Kombinatorische schutzgruppestrategie für multifunktionelle molekule
US20050287596A9 (en) * 1998-06-26 2005-12-29 Braisted Andrew C Novel ligands and libraries of ligands
WO2002010398A2 (en) * 2000-07-31 2002-02-07 Hahn Frederick M Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
US7172886B2 (en) * 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
EP1502102B1 (en) * 2002-03-11 2009-01-14 caprotec bioanalytics GmbH Compounds and methods for analyzing the proteome
EP2259068B1 (en) * 2003-01-16 2013-08-14 caprotec bioanalytics GmbH Capture compounds and methods for analyzing the proteome
AU2006325040B2 (en) 2005-06-07 2012-04-19 Dsm Nutritional Products Ag Eukaryotic microorganisms for producing lipids and antioxidants
WO2007044688A1 (en) * 2005-10-07 2007-04-19 The Regents Of The University Of California Nucleic acids encoding modified cytochrome p450 enzymes and methods of use thereof
US8114645B2 (en) * 2006-05-19 2012-02-14 The Regents Of The University Of California Methods for increasing isoprenoid and isoprenoid precursor production by modulating fatty acid levels
CA2652801C (en) * 2006-05-26 2018-05-22 Amyris Biotechnologies, Inc. Apparatus for making bio-organic compounds
CA2659603C (en) * 2006-08-01 2017-03-07 Ocean Nutrition Canada Limited Oil producing microbes and methods of modification thereof
EP2066778B1 (en) * 2006-09-26 2016-01-27 The Regents of The University of California Production of isoprenoids and isoprenoid precursors
US8234641B2 (en) * 2006-10-17 2012-07-31 Managelq, Inc. Compliance-based adaptations in managed virtual systems
WO2008094546A2 (en) * 2007-01-31 2008-08-07 The Regents Of The University Of California Genetically modified host cells for increased p450 activity levels and methods of use thereof
WO2009006386A2 (en) 2007-06-29 2009-01-08 The Regents Of The University Of California Host cells and methods for producing isoprenyl alkanoates
US8067632B2 (en) * 2007-07-26 2011-11-29 The Board Of Trustees Of The Leland Stanford Junior University Process to produce prostratin and structural or functional analogs thereof
WO2009064910A2 (en) * 2007-11-13 2009-05-22 Synthetic Genomics, Inc. Dimethyloctane as an advanced biofuel
CN105368764B (zh) 2007-12-13 2019-03-12 丹尼斯科美国公司 用于生产异戊二烯的组合物和方法
BRPI0906620A8 (pt) * 2008-01-31 2016-01-19 Amyris Biotechnologies Inc Método para quanitificar um cofator e um metabólico da via do mevalonato, extrato de um célula, e, método de fermentação
US20090253174A1 (en) * 2008-04-08 2009-10-08 Zach Serber Expression of Heterologous Sequences
WO2009132220A2 (en) * 2008-04-23 2009-10-29 Danisco Us Inc. Isoprene synthase variants for improved microbial production of isoprene
CA2725175A1 (en) * 2008-05-23 2009-11-26 Cibus Oils, Llc Production of squalene using yeast
JP5624980B2 (ja) * 2008-06-30 2014-11-12 ザ・グッドイヤー・タイヤ・アンド・ラバー・カンパニーＴｈｅ Ｇｏｏｄｙｅａｒｔｉｒｅ ＆ Ｒｕｂｂｅｒ Ｃｏｍｐａｎｙ 再生資源に由来するイソプレンの重合体
MX318543B (es) 2008-07-02 2014-03-18 Danisco Us Inc Composiciones y metodos para producir isopreno libre de hidrocarburos de c5 bajo condicion de desacoplamiento y/o intervalos de operacion seguros.
CA2737082A1 (en) * 2008-09-15 2010-03-18 Danisco Us Inc. Increased isoprene production using mevalonate kinase and isoprene synthase
WO2010031062A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Increased isoprene production using the archaeal lower mevalonate pathway
KR101700296B1 (ko) 2008-10-28 2017-01-26 알이지 라이프 사이언시스, 엘엘씨 지방족 알코올들을 생산하기 위한 방법들과 조성물들
AU2010238770A1 (en) 2009-04-23 2011-11-03 Danisco Us Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
HUE034245T2 (en) * 2009-11-23 2018-02-28 Nucelis Llc Methods and preparations for the preparation of squalene using yeast
EP2575432B1 (en) 2010-06-02 2019-08-14 Evolva, Inc. Recombinant production of steviol glycosides
CA2816306A1 (en) 2010-10-27 2012-05-03 Danisco Us Inc. Isoprene synthase variants for improved production of isoprene
CA3128532A1 (en) 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
IN2014CN01129A (https=) 2011-08-08 2015-04-10 Evolva Sa
US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene
WO2013181647A2 (en) 2012-06-01 2013-12-05 Danisco Us Inc. Compositions and methods of producing isoprene and/or industrrial bio-products using anaerobic microorganisms
JP5827601B2 (ja) * 2012-07-04 2015-12-02 住友ゴム工業株式会社 特定の蛋白質の発現を光応答転写因子により調整する方法、光応答転写因子をコードする遺伝子が導入されたイソプレノイド産生植物、及び該イソプレノイド産生植物を用いたポリイソプレノイドの製造方法
MY199452A (en) 2013-02-06 2023-10-30 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
BR112015019160A2 (pt) 2013-02-11 2017-08-22 Dalgaard Mikkelsen Michael Produção de glicosídeos de esteviol em hospedeiros recombinantes
EP2970926B1 (en) 2013-03-13 2018-01-31 DSM Nutritional Products AG Engineering microorganisms
JP6282813B2 (ja) * 2013-07-16 2018-02-21 住友ゴム工業株式会社 ヒドロキシメチルグルタリルＣｏＡレダクターゼの発現をｂＺＩＰ型転写因子により調整する方法、ｂＺＩＰ型転写因子をコードする遺伝子が導入されたイソプレノイド産生植物、及び該イソプレノイド産生植物を用いたポリイソプレノイドの製造方法
CA2957331A1 (en) 2014-08-11 2016-02-18 Evolva Sa Production of steviol glycosides in recombinant hosts
CA2960693A1 (en) 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3250686A1 (en) 2015-01-30 2017-12-06 Evolva SA Production of steviol glycosides in recombinant hosts
WO2016146711A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
MX2018001620A (es) 2015-08-07 2018-06-11 Evolva Sa Produccion de glucosidos de esteviol en hospedadores recombinantes.
US10982249B2 (en) 2016-04-13 2021-04-20 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3458599A1 (en) 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2019055325A2 (en) * 2017-09-12 2019-03-21 Biocapital Holdings, Llc BIOOGICAL DEVICES AND METHODS OF USING THE SAME TO PRODUCE STEVIOL GLYCOSIDES
WO2019090286A2 (en) * 2017-11-06 2019-05-09 The Regents Of The University Of California Production of valerenic acid in fungal cells
WO2019113387A1 (en) 2017-12-07 2019-06-13 Zymergen Inc. Engineered biosynthetic pathways for production of (6e)-8-hydroxygeraniol by fermentation
JP2021506316A (ja) 2017-12-21 2021-02-22 ザイマージェン インコーポレイテッド ネペタラクトールオキシドレダクターゼ、ネペタラクトールシンターゼ、及びネペタラクトンを産生する能力がある微生物
CN109777815B (zh) * 2019-03-28 2021-10-29 昆明理工大学 HMG-CoA合成酶基因RKHMGCS及其应用
NL2032683B1 (en) * 2022-07-18 2024-01-26 Sestina Bio Llc Bioproduction of isoprenoids
EP4565691A1 (en) * 2022-11-14 2025-06-11 Agency for Science, Technology and Research Biosynthesis of isoprenoid
WO2025217176A1 (en) * 2024-04-08 2025-10-16 Board Of Trustees Of Michigan State University Genetically engineered bacteria as a chassis host for high yield terpenoid production

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5460949A (en) 1990-11-15 1995-10-24 Amoco Corporation Method and composition for increasing the accumulation of squalene and specific sterols in yeast
KR20060099543A (ko) 1994-12-09 2006-09-19 임페리얼 컬리지 이노베이션스 리미티드 유전자의 동정
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
JP2002515738A (ja) 1996-01-23 2002-05-28 アフィメトリックス，インコーポレイティド 核酸分析法
US6080849A (en) 1997-09-10 2000-06-27 Vion Pharmaceuticals, Inc. Genetically modified tumor-targeted bacteria with reduced virulence
US6033861A (en) 1997-11-19 2000-03-07 Incyte Genetics, Inc. Methods for obtaining nucleic acid containing a mutation
CN1308623A (zh) * 1998-07-06 2001-08-15 伊斯曼化学公司 生产维生素e的方法
JP4579415B2 (ja) 1998-07-06 2010-11-10 ディーシーブイ・インコーポレイテッド・ドゥーイング・ビジネス・アズ・バイオ−テクニカル・リソーシィズ ビタミン製造方法
US6531303B1 (en) 1998-07-06 2003-03-11 Arkion Life Sciences Llc Method of producing geranylgeraniol
AR022383A1 (es) * 1998-09-18 2002-09-04 Univ Kentucky Res Found Sintasas
US6645747B1 (en) 1999-09-21 2003-11-11 E. I. Du Pont De Nemours And Company Cis-prenyltransferases from plants
GB9925453D0 (en) * 1999-10-27 1999-12-29 Univ Bristol Increasing isoprenoid biosynthesis
WO2002010398A2 (en) * 2000-07-31 2002-02-07 Hahn Frederick M Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
AU2001291114A1 (en) 2000-09-19 2002-04-02 Microbia, Inc. Modulation of secondary metabolite production by zinc binuclear cluster proteins
US20040063182A1 (en) 2000-12-28 2004-04-01 Chikara Ohto Process for producing prenyl alcohol
JP3838033B2 (ja) 2000-12-28 2006-10-25 トヨタ自動車株式会社 プレニルアルコールの製造方法
US7238514B2 (en) * 2001-01-05 2007-07-03 William Marsh Rice University Diterpene-producing unicellular organism
GB0105924D0 (en) 2001-03-09 2001-04-25 Microscience Ltd Promoter
AU2002316966B2 (en) * 2001-06-06 2007-05-17 Dsm Ip Assets B.V. Improved isoprenoid production
US20040110259A1 (en) 2001-08-03 2004-06-10 Baugh Mariah R Drug metabolizing enzymes
US7172886B2 (en) * 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
US7192751B2 (en) 2001-12-06 2007-03-20 The Regents Of The University Of California Biosynthesis of amorpha-4,11-diene
US7927794B2 (en) * 2003-09-29 2011-04-19 The Regents Of The University Of California Methods for identifying a biosynthetic pathway gene product

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CN106119112A (zh) * 2008-03-27 2016-11-16 基因组股份公司 用于产生己二酸和其他化合物的微生物
CN106119112B (zh) * 2008-03-27 2021-07-13 基因组股份公司 用于产生己二酸和其他化合物的微生物
US11186854B2 (en) 2008-05-16 2021-11-30 Genomatica, Inc. Methods and compositions for producing hydrocarbons
US10563231B2 (en) 2008-05-16 2020-02-18 Genomatica, Inc. Methods and compositions for producing hydrocarbons
CN105112455B (zh) * 2008-05-16 2016-08-17 Reg生命科学有限责任公司 产生碳氢化合物的方法和组合物
CN105112454A (zh) * 2008-05-16 2015-12-02 Reg生命科学有限责任公司 产生碳氢化合物的方法和组合物
CN105112454B (zh) * 2008-05-16 2017-01-18 Reg生命科学有限责任公司 产生碳氢化合物的方法和组合物
CN104039974B (zh) * 2011-11-09 2016-10-12 阿迈瑞斯公司 乙酰辅酶a衍生的类异戊二烯的生产
CN104039974A (zh) * 2011-11-09 2014-09-10 阿迈瑞斯公司 乙酰辅酶a衍生的类异戊二烯的生产
US9816124B2 (en) 2013-02-01 2017-11-14 Tianjin Institute Of Industrial Biotechnology, Chinese Academy Of Sciences Recombinant microorganism for preparing terpenoid and method for constructing recombinant microorganism
CN103602626B (zh) * 2013-10-12 2016-02-10 中国科学院青岛生物能源与过程研究所 一种以油脂为原料生产甲羟戊酸的方法及其构建的基因工程菌
CN103602626A (zh) * 2013-10-12 2014-02-26 中国科学院青岛生物能源与过程研究所 一种以油脂为原料生产甲羟戊酸的方法及其构建的基因工程菌
CN106367508A (zh) * 2016-09-13 2017-02-01 东北农业大学 一种用于鉴定杂合型类异戊二烯化合物产生菌的简并引物与应用
WO2023227104A1 (zh) * 2022-05-26 2023-11-30 牛津大学(苏州)科技有限公司 一种用于合成萜烯的共培养系统和方法
CN116925991A (zh) * 2023-07-28 2023-10-24 天津大学 高产甲羟戊酸的重组盐单胞菌菌株及构建方法与应用
WO2025108471A1 (zh) * 2023-11-24 2025-05-30 牛津大学(苏州)科技有限公司 用于合成目标化合物的共培养系统和方法

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Publication number Publication date
US20060079476A1 (en) 2006-04-13
EP1765418B1 (en) 2011-12-14
US20090004724A1 (en) 2009-01-01
BRPI0510115B1 (pt) 2022-09-27
MXPA06013502A (es) 2007-03-01
US7670825B2 (en) 2010-03-02
JP4926061B2 (ja) 2012-05-09
EP1765418A4 (en) 2008-07-02
CA2567547A1 (en) 2006-08-17
ATE537269T1 (de) 2011-12-15
EP2365090A1 (en) 2011-09-14
US7183089B2 (en) 2007-02-27
CA2567547C (en) 2012-10-23
WO2006085899A3 (en) 2007-05-18
WO2006085899A2 (en) 2006-08-17
AU2005327292B2 (en) 2010-11-04
BRPI0510115A (pt) 2007-09-25
ES2379368T3 (es) 2012-04-25
JP2008500063A (ja) 2008-01-10
EP1765418A2 (en) 2007-03-28
AU2005327292A1 (en) 2006-08-17

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