BRPI0510115A - método para intensificação da produção de compostos de isoprenóide - Google Patents

método para intensificação da produção de compostos de isoprenóide

Info

Publication number
BRPI0510115A
BRPI0510115A BRPI0510115-8A BRPI0510115A BRPI0510115A BR PI0510115 A BRPI0510115 A BR PI0510115A BR PI0510115 A BRPI0510115 A BR PI0510115A BR PI0510115 A BRPI0510115 A BR PI0510115A
Authority
BR
Brazil
Prior art keywords
coa
hmg
isoprenoid
present
invention further
Prior art date
Application number
BRPI0510115-8A
Other languages
English (en)
Inventor
Jay D Keasling
Jack D Newman
Douglas J Pitera
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36793476&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI0510115(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ California filed Critical Univ California
Publication of BRPI0510115A publication Critical patent/BRPI0510115A/pt
Publication of BRPI0510115B1 publication Critical patent/BRPI0510115B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Steroid Compounds (AREA)

Abstract

MéTODO PARA INTENSIFICAçãO DA PRODUçãO DE COMPOSTOS DE ISOPRENóIDE. A presente invenção refere-se a métodos de produção de um isoprenóide ou precursor de isoprenóide em uma célula hospedeira geneticamente modificada. Os métodos geralmente envolvem modulação do nível de hidróximetilglutaril-CoA (HMG-CoA) na célula, de modo que o nível de HMG-CoA não é tóxico para a célula e/ou não inibe substancialmente o crescimento celular, mas é mantido em um nível que proporciona produção em alto nível de mevalonato, IPP e outros produtos a jusante de um isoprenóide ou via de isoprenóide (por exemplo, poliprenila) difosfatos e compostos de isoprenóide. A presente invenção ainda proporciona células hospedeiras geneticamente modificadas que são adequadas para uso no método em questão. A presente invenção ainda proporciona estruturas de ácido nucléico recambinantes para uso na geração de uma célula hospedeira geneticamente modificada em questão, incluindo estruturas de ácido nucléico recombinantes compreendendo seqüências de nucleotídeo que codificam uma ou mais enzimas da via de mevalanato e vetores recombinantes (por exemplo, vetores de expressão recombinantes) compreendendo as mesmas. A presente invenção ainda proporciona métodos para a identificação de ácidos nucléicos que codificam variantes de reductase de HMG-CoA (HMGR) que proporcionam alívio da toxicidade acúmulo de HMG-CoA-induzida. A presente invenção ainda proporciona métodos para a identificação de agentes que reduzem o acúmulo intracelular de HMG-CoA.
BRPI0510115-8A 2004-05-21 2005-05-20 Método para produzir um isoprenóide ou precursor de isoprenóide através de uma via de mevalonato em escherichia coli e célula hospedeira geneticamente modificada que os produz BRPI0510115B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US57349204P 2004-05-21 2004-05-21
US60/573,492 2004-05-21
PCT/US2005/017874 WO2006085899A2 (en) 2004-05-21 2005-05-20 Method for enhancing production of isoprenoid compounds

Publications (2)

Publication Number Publication Date
BRPI0510115A true BRPI0510115A (pt) 2007-09-25
BRPI0510115B1 BRPI0510115B1 (pt) 2022-09-27

Family

ID=36793476

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0510115-8A BRPI0510115B1 (pt) 2004-05-21 2005-05-20 Método para produzir um isoprenóide ou precursor de isoprenóide através de uma via de mevalonato em escherichia coli e célula hospedeira geneticamente modificada que os produz

Country Status (11)

Country Link
US (2) US7183089B2 (pt)
EP (2) EP1765418B1 (pt)
JP (1) JP4926061B2 (pt)
CN (1) CN101023181A (pt)
AT (1) ATE537269T1 (pt)
AU (1) AU2005327292B2 (pt)
BR (1) BRPI0510115B1 (pt)
CA (1) CA2567547C (pt)
ES (1) ES2379368T3 (pt)
MX (1) MXPA06013502A (pt)
WO (1) WO2006085899A2 (pt)

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Also Published As

Publication number Publication date
US20060079476A1 (en) 2006-04-13
JP2008500063A (ja) 2008-01-10
US7670825B2 (en) 2010-03-02
US7183089B2 (en) 2007-02-27
WO2006085899A2 (en) 2006-08-17
CN101023181A (zh) 2007-08-22
US20090004724A1 (en) 2009-01-01
AU2005327292A1 (en) 2006-08-17
CA2567547C (en) 2012-10-23
EP2365090A1 (en) 2011-09-14
JP4926061B2 (ja) 2012-05-09
ES2379368T3 (es) 2012-04-25
CA2567547A1 (en) 2006-08-17
WO2006085899A3 (en) 2007-05-18
MXPA06013502A (es) 2007-03-01
AU2005327292B2 (en) 2010-11-04
ATE537269T1 (de) 2011-12-15
EP1765418A2 (en) 2007-03-28
BRPI0510115B1 (pt) 2022-09-27
EP1765418B1 (en) 2011-12-14
EP1765418A4 (en) 2008-07-02

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Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 20/05/2005, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF, QUE DETERMINA A ALTERACAO DO PRAZO DE CONCESSAO.