ES2379368T3 - Método para aumentar la producción de compuestos isoprenoides - Google Patents
Método para aumentar la producción de compuestos isoprenoides Download PDFInfo
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- ES2379368T3 ES2379368T3 ES05857432T ES05857432T ES2379368T3 ES 2379368 T3 ES2379368 T3 ES 2379368T3 ES 05857432 T ES05857432 T ES 05857432T ES 05857432 T ES05857432 T ES 05857432T ES 2379368 T3 ES2379368 T3 ES 2379368T3
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
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- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- Biochemistry (AREA)
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- General Health & Medical Sciences (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Steroid Compounds (AREA)
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| US57349204P | 2004-05-21 | 2004-05-21 | |
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| PCT/US2005/017874 WO2006085899A2 (en) | 2004-05-21 | 2005-05-20 | Method for enhancing production of isoprenoid compounds |
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| ES2379368T3 true ES2379368T3 (es) | 2012-04-25 |
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| ES05857432T Expired - Lifetime ES2379368T3 (es) | 2004-05-21 | 2005-05-20 | Método para aumentar la producción de compuestos isoprenoides |
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| US (2) | US7183089B2 (https=) |
| EP (2) | EP2365090A1 (https=) |
| JP (1) | JP4926061B2 (https=) |
| CN (1) | CN101023181A (https=) |
| AT (1) | ATE537269T1 (https=) |
| AU (1) | AU2005327292B2 (https=) |
| BR (1) | BRPI0510115B1 (https=) |
| CA (1) | CA2567547C (https=) |
| ES (1) | ES2379368T3 (https=) |
| MX (1) | MXPA06013502A (https=) |
| WO (1) | WO2006085899A2 (https=) |
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| ATE286063T1 (de) * | 1996-04-17 | 2005-01-15 | Hubert Dr Koester | Kombinatorische schutzgruppestrategie für multifunktionelle molekule |
| US20050287596A9 (en) * | 1998-06-26 | 2005-12-29 | Braisted Andrew C | Novel ligands and libraries of ligands |
| WO2002010398A2 (en) * | 2000-07-31 | 2002-02-07 | Hahn Frederick M | Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms |
| US7172886B2 (en) * | 2001-12-06 | 2007-02-06 | The Regents Of The University Of California | Biosynthesis of isopentenyl pyrophosphate |
| EP1502102B1 (en) * | 2002-03-11 | 2009-01-14 | caprotec bioanalytics GmbH | Compounds and methods for analyzing the proteome |
| EP2259068B1 (en) * | 2003-01-16 | 2013-08-14 | caprotec bioanalytics GmbH | Capture compounds and methods for analyzing the proteome |
| AU2006325040B2 (en) | 2005-06-07 | 2012-04-19 | Dsm Nutritional Products Ag | Eukaryotic microorganisms for producing lipids and antioxidants |
| WO2007044688A1 (en) * | 2005-10-07 | 2007-04-19 | The Regents Of The University Of California | Nucleic acids encoding modified cytochrome p450 enzymes and methods of use thereof |
| US8114645B2 (en) * | 2006-05-19 | 2012-02-14 | The Regents Of The University Of California | Methods for increasing isoprenoid and isoprenoid precursor production by modulating fatty acid levels |
| CA2652801C (en) * | 2006-05-26 | 2018-05-22 | Amyris Biotechnologies, Inc. | Apparatus for making bio-organic compounds |
| CA2659603C (en) * | 2006-08-01 | 2017-03-07 | Ocean Nutrition Canada Limited | Oil producing microbes and methods of modification thereof |
| EP2066778B1 (en) * | 2006-09-26 | 2016-01-27 | The Regents of The University of California | Production of isoprenoids and isoprenoid precursors |
| US8234641B2 (en) * | 2006-10-17 | 2012-07-31 | Managelq, Inc. | Compliance-based adaptations in managed virtual systems |
| WO2008094546A2 (en) * | 2007-01-31 | 2008-08-07 | The Regents Of The University Of California | Genetically modified host cells for increased p450 activity levels and methods of use thereof |
| WO2009006386A2 (en) | 2007-06-29 | 2009-01-08 | The Regents Of The University Of California | Host cells and methods for producing isoprenyl alkanoates |
| US8067632B2 (en) * | 2007-07-26 | 2011-11-29 | The Board Of Trustees Of The Leland Stanford Junior University | Process to produce prostratin and structural or functional analogs thereof |
| WO2009064910A2 (en) * | 2007-11-13 | 2009-05-22 | Synthetic Genomics, Inc. | Dimethyloctane as an advanced biofuel |
| CN105368764B (zh) | 2007-12-13 | 2019-03-12 | 丹尼斯科美国公司 | 用于生产异戊二烯的组合物和方法 |
| BRPI0906620A8 (pt) * | 2008-01-31 | 2016-01-19 | Amyris Biotechnologies Inc | Método para quanitificar um cofator e um metabólico da via do mevalonato, extrato de um célula, e, método de fermentação |
| CN102066551B (zh) * | 2008-03-27 | 2013-10-09 | 基因组股份公司 | 用于产生己二酸和其他化合物的微生物 |
| US20090253174A1 (en) * | 2008-04-08 | 2009-10-08 | Zach Serber | Expression of Heterologous Sequences |
| WO2009132220A2 (en) * | 2008-04-23 | 2009-10-29 | Danisco Us Inc. | Isoprene synthase variants for improved microbial production of isoprene |
| JP2011520455A (ja) | 2008-05-16 | 2011-07-21 | エルエス９・インコーポレイテッド | 炭化水素を製造するための方法および組成物 |
| CA2725175A1 (en) * | 2008-05-23 | 2009-11-26 | Cibus Oils, Llc | Production of squalene using yeast |
| JP5624980B2 (ja) * | 2008-06-30 | 2014-11-12 | ザ・グッドイヤー・タイヤ・アンド・ラバー・カンパニーＴｈｅ Ｇｏｏｄｙｅａｒｔｉｒｅ ＆ Ｒｕｂｂｅｒ Ｃｏｍｐａｎｙ | 再生資源に由来するイソプレンの重合体 |
| MX318543B (es) | 2008-07-02 | 2014-03-18 | Danisco Us Inc | Composiciones y metodos para producir isopreno libre de hidrocarburos de c5 bajo condicion de desacoplamiento y/o intervalos de operacion seguros. |
| CA2737082A1 (en) * | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Increased isoprene production using mevalonate kinase and isoprene synthase |
| WO2010031062A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Increased isoprene production using the archaeal lower mevalonate pathway |
| KR101700296B1 (ko) | 2008-10-28 | 2017-01-26 | 알이지 라이프 사이언시스, 엘엘씨 | 지방족 알코올들을 생산하기 위한 방법들과 조성물들 |
| AU2010238770A1 (en) | 2009-04-23 | 2011-11-03 | Danisco Us Inc. | Three-dimensional structure of isoprene synthase and its use thereof for generating variants |
| HUE034245T2 (en) * | 2009-11-23 | 2018-02-28 | Nucelis Llc | Methods and preparations for the preparation of squalene using yeast |
| EP2575432B1 (en) | 2010-06-02 | 2019-08-14 | Evolva, Inc. | Recombinant production of steviol glycosides |
| CA2816306A1 (en) | 2010-10-27 | 2012-05-03 | Danisco Us Inc. | Isoprene synthase variants for improved production of isoprene |
| CA3128532A1 (en) | 2011-08-08 | 2013-02-14 | Evolva Sa | Recombinant production of steviol glycosides |
| IN2014CN01129A (https=) | 2011-08-08 | 2015-04-10 | Evolva Sa | |
| CN104039974B (zh) * | 2011-11-09 | 2016-10-12 | 阿迈瑞斯公司 | 乙酰辅酶a衍生的类异戊二烯的生产 |
| US9163263B2 (en) | 2012-05-02 | 2015-10-20 | The Goodyear Tire & Rubber Company | Identification of isoprene synthase variants with improved properties for the production of isoprene |
| WO2013181647A2 (en) | 2012-06-01 | 2013-12-05 | Danisco Us Inc. | Compositions and methods of producing isoprene and/or industrrial bio-products using anaerobic microorganisms |
| JP5827601B2 (ja) * | 2012-07-04 | 2015-12-02 | 住友ゴム工業株式会社 | 特定の蛋白質の発現を光応答転写因子により調整する方法、光応答転写因子をコードする遺伝子が導入されたイソプレノイド産生植物、及び該イソプレノイド産生植物を用いたポリイソプレノイドの製造方法 |
| CN103087972B (zh) | 2013-02-01 | 2014-11-05 | 中国科学院天津工业生物技术研究所 | 生产萜类化合物的重组微生物及构建方法 |
| MY199452A (en) | 2013-02-06 | 2023-10-30 | Evolva Sa | Methods for improved production of rebaudioside d and rebaudioside m |
| BR112015019160A2 (pt) | 2013-02-11 | 2017-08-22 | Dalgaard Mikkelsen Michael | Produção de glicosídeos de esteviol em hospedeiros recombinantes |
| EP2970926B1 (en) | 2013-03-13 | 2018-01-31 | DSM Nutritional Products AG | Engineering microorganisms |
| JP6282813B2 (ja) * | 2013-07-16 | 2018-02-21 | 住友ゴム工業株式会社 | ヒドロキシメチルグルタリルＣｏＡレダクターゼの発現をｂＺＩＰ型転写因子により調整する方法、ｂＺＩＰ型転写因子をコードする遺伝子が導入されたイソプレノイド産生植物、及び該イソプレノイド産生植物を用いたポリイソプレノイドの製造方法 |
| CN103602626B (zh) * | 2013-10-12 | 2016-02-10 | 中国科学院青岛生物能源与过程研究所 | 一种以油脂为原料生产甲羟戊酸的方法及其构建的基因工程菌 |
| CA2957331A1 (en) | 2014-08-11 | 2016-02-18 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| CA2960693A1 (en) | 2014-09-09 | 2016-03-17 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| EP3250686A1 (en) | 2015-01-30 | 2017-12-06 | Evolva SA | Production of steviol glycosides in recombinant hosts |
| WO2016146711A1 (en) | 2015-03-16 | 2016-09-22 | Dsm Ip Assets B.V. | Udp-glycosyltransferases |
| MX2018001620A (es) | 2015-08-07 | 2018-06-11 | Evolva Sa | Produccion de glucosidos de esteviol en hospedadores recombinantes. |
| US10982249B2 (en) | 2016-04-13 | 2021-04-20 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| EP3458599A1 (en) | 2016-05-16 | 2019-03-27 | Evolva SA | Production of steviol glycosides in recombinant hosts |
| CN106367508B (zh) * | 2016-09-13 | 2019-10-29 | 东北农业大学 | 一种用于鉴定杂合型类异戊二烯化合物产生菌的简并引物与应用 |
| WO2018083338A1 (en) | 2016-11-07 | 2018-05-11 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| WO2019055325A2 (en) * | 2017-09-12 | 2019-03-21 | Biocapital Holdings, Llc | BIOOGICAL DEVICES AND METHODS OF USING THE SAME TO PRODUCE STEVIOL GLYCOSIDES |
| WO2019090286A2 (en) * | 2017-11-06 | 2019-05-09 | The Regents Of The University Of California | Production of valerenic acid in fungal cells |
| WO2019113387A1 (en) | 2017-12-07 | 2019-06-13 | Zymergen Inc. | Engineered biosynthetic pathways for production of (6e)-8-hydroxygeraniol by fermentation |
| JP2021506316A (ja) | 2017-12-21 | 2021-02-22 | ザイマージェン インコーポレイテッド | ネペタラクトールオキシドレダクターゼ、ネペタラクトールシンターゼ、及びネペタラクトンを産生する能力がある微生物 |
| CN109777815B (zh) * | 2019-03-28 | 2021-10-29 | 昆明理工大学 | HMG-CoA合成酶基因RKHMGCS及其应用 |
| CN117165502A (zh) * | 2022-05-26 | 2023-12-05 | 牛津大学(苏州)科技有限公司 | 一种合成萜类化合物的共培养系统和方法 |
| NL2032683B1 (en) * | 2022-07-18 | 2024-01-26 | Sestina Bio Llc | Bioproduction of isoprenoids |
| EP4565691A1 (en) * | 2022-11-14 | 2025-06-11 | Agency for Science, Technology and Research | Biosynthesis of isoprenoid |
| CN116925991B (zh) * | 2023-07-28 | 2024-08-09 | 天津大学 | 高产甲羟戊酸的重组盐单胞菌菌株及构建方法与应用 |
| CN120041291A (zh) * | 2023-11-24 | 2025-05-27 | 牛津大学(苏州)科技有限公司 | 用于合成目标化合物的共培养系统和方法 |
| WO2025217176A1 (en) * | 2024-04-08 | 2025-10-16 | Board Of Trustees Of Michigan State University | Genetically engineered bacteria as a chassis host for high yield terpenoid production |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5460949A (en) | 1990-11-15 | 1995-10-24 | Amoco Corporation | Method and composition for increasing the accumulation of squalene and specific sterols in yeast |
| KR20060099543A (ko) | 1994-12-09 | 2006-09-19 | 임페리얼 컬리지 이노베이션스 리미티드 | 유전자의 동정 |
| US6740506B2 (en) | 1995-12-07 | 2004-05-25 | Diversa Corporation | End selection in directed evolution |
| US6171820B1 (en) | 1995-12-07 | 2001-01-09 | Diversa Corporation | Saturation mutagenesis in directed evolution |
| JP2002515738A (ja) | 1996-01-23 | 2002-05-28 | アフィメトリックス，インコーポレイティド | 核酸分析法 |
| US6080849A (en) | 1997-09-10 | 2000-06-27 | Vion Pharmaceuticals, Inc. | Genetically modified tumor-targeted bacteria with reduced virulence |
| US6033861A (en) | 1997-11-19 | 2000-03-07 | Incyte Genetics, Inc. | Methods for obtaining nucleic acid containing a mutation |
| CN1308623A (zh) * | 1998-07-06 | 2001-08-15 | 伊斯曼化学公司 | 生产维生素e的方法 |
| JP4579415B2 (ja) | 1998-07-06 | 2010-11-10 | ディーシーブイ・インコーポレイテッド・ドゥーイング・ビジネス・アズ・バイオ−テクニカル・リソーシィズ | ビタミン製造方法 |
| US6531303B1 (en) | 1998-07-06 | 2003-03-11 | Arkion Life Sciences Llc | Method of producing geranylgeraniol |
| AR022383A1 (es) * | 1998-09-18 | 2002-09-04 | Univ Kentucky Res Found | Sintasas |
| US6645747B1 (en) | 1999-09-21 | 2003-11-11 | E. I. Du Pont De Nemours And Company | Cis-prenyltransferases from plants |
| GB9925453D0 (en) * | 1999-10-27 | 1999-12-29 | Univ Bristol | Increasing isoprenoid biosynthesis |
| WO2002010398A2 (en) * | 2000-07-31 | 2002-02-07 | Hahn Frederick M | Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms |
| AU2001291114A1 (en) | 2000-09-19 | 2002-04-02 | Microbia, Inc. | Modulation of secondary metabolite production by zinc binuclear cluster proteins |
| US20040063182A1 (en) | 2000-12-28 | 2004-04-01 | Chikara Ohto | Process for producing prenyl alcohol |
| JP3838033B2 (ja) | 2000-12-28 | 2006-10-25 | トヨタ自動車株式会社 | プレニルアルコールの製造方法 |
| US7238514B2 (en) * | 2001-01-05 | 2007-07-03 | William Marsh Rice University | Diterpene-producing unicellular organism |
| GB0105924D0 (en) | 2001-03-09 | 2001-04-25 | Microscience Ltd | Promoter |
| AU2002316966B2 (en) * | 2001-06-06 | 2007-05-17 | Dsm Ip Assets B.V. | Improved isoprenoid production |
| US20040110259A1 (en) | 2001-08-03 | 2004-06-10 | Baugh Mariah R | Drug metabolizing enzymes |
| US7172886B2 (en) * | 2001-12-06 | 2007-02-06 | The Regents Of The University Of California | Biosynthesis of isopentenyl pyrophosphate |
| US7192751B2 (en) | 2001-12-06 | 2007-03-20 | The Regents Of The University Of California | Biosynthesis of amorpha-4,11-diene |
| US7927794B2 (en) * | 2003-09-29 | 2011-04-19 | The Regents Of The University Of California | Methods for identifying a biosynthetic pathway gene product |
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| WO2006085899A2 (en) | 2006-08-17 |
| AU2005327292B2 (en) | 2010-11-04 |
| BRPI0510115A (pt) | 2007-09-25 |
| JP2008500063A (ja) | 2008-01-10 |
| EP1765418A2 (en) | 2007-03-28 |
| AU2005327292A1 (en) | 2006-08-17 |
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