JP4926061B2 - イソプレノイド化合物の産生を促進する方法 - Google Patents

イソプレノイド化合物の産生を促進する方法 Download PDF

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JP4926061B2
JP4926061B2 JP2007527501A JP2007527501A JP4926061B2 JP 4926061 B2 JP4926061 B2 JP 4926061B2 JP 2007527501 A JP2007527501 A JP 2007527501A JP 2007527501 A JP2007527501 A JP 2007527501A JP 4926061 B2 JP4926061 B2 JP 4926061B2
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coa
cell
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JP2008500063A (ja
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ジェイ ディー． キースリング
ジャック ディー． ニューマン
ダグラス ジェイ． ピテラ
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University of California
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
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  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
JP2007527501A 2004-05-21 2005-05-20 イソプレノイド化合物の産生を促進する方法 Expired - Lifetime JP4926061B2 (ja)

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Application Number Priority Date Filing Date Title
US57349204P 2004-05-21 2004-05-21
US60/573,492 2004-05-21
PCT/US2005/017874 WO2006085899A2 (en) 2004-05-21 2005-05-20 Method for enhancing production of isoprenoid compounds

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JP2008500063A JP2008500063A (ja) 2008-01-10
JP2008500063A5 JP2008500063A5 (https=) 2008-04-24
JP4926061B2 true JP4926061B2 (ja) 2012-05-09

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US (2) US7183089B2 (https=)
EP (2) EP2365090A1 (https=)
JP (1) JP4926061B2 (https=)
CN (1) CN101023181A (https=)
AT (1) ATE537269T1 (https=)
AU (1) AU2005327292B2 (https=)
BR (1) BRPI0510115B1 (https=)
CA (1) CA2567547C (https=)
ES (1) ES2379368T3 (https=)
MX (1) MXPA06013502A (https=)
WO (1) WO2006085899A2 (https=)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2462497A (en) * 1996-04-17 1997-11-19 Hubert Koster A combinatorial protecting group strategy for multifunctional molecules
US20050287596A9 (en) * 1998-06-26 2005-12-29 Braisted Andrew C Novel ligands and libraries of ligands
AU2001278098A1 (en) * 2000-07-31 2002-02-13 Frederick M. Hahn Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
US7172886B2 (en) * 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
AU2003224674A1 (en) * 2002-03-11 2003-09-29 Hk Pharmaceuticals, Inc. Compounds and methods for analyzing the proteome
WO2004064972A2 (en) * 2003-01-16 2004-08-05 Hk Pharmaceuticals, Inc. Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
ES2626018T3 (es) 2005-06-07 2017-07-21 Dsm Nutritional Products Ag Microorganismos eucariotas para la producción de lípidos y antioxidantes
EP1931770A4 (en) * 2005-10-07 2009-11-25 Univ California NUCLEIC ACIDS CODING FOR MODIFIED CYTOCHROM P450 ENZYME AND METHOD OF USE THEREOF
WO2007136847A2 (en) * 2006-05-19 2007-11-29 The Regents Of The University Of California Methods for increasing isoprenoid and isoprenoid precursor production by modulating fatty acid levels
CN105331518A (zh) * 2006-05-26 2016-02-17 阿迈瑞斯公司 制造生物有机化合物的设备
CN101981201A (zh) * 2006-08-01 2011-02-23 加拿大海洋营养食品有限公司 产油微生物及改良这些微生物的方法
DK2066778T3 (en) * 2006-09-26 2016-04-18 Amyris Biotechnologies Inc PREPARATION OF ISOPRENOIDS AND ISOPRENOID PRECURSORS
US8234641B2 (en) * 2006-10-17 2012-07-31 Managelq, Inc. Compliance-based adaptations in managed virtual systems
WO2008094546A2 (en) * 2007-01-31 2008-08-07 The Regents Of The University Of California Genetically modified host cells for increased p450 activity levels and methods of use thereof
WO2009006386A2 (en) 2007-06-29 2009-01-08 The Regents Of The University Of California Host cells and methods for producing isoprenyl alkanoates
US8067632B2 (en) * 2007-07-26 2011-11-29 The Board Of Trustees Of The Leland Stanford Junior University Process to produce prostratin and structural or functional analogs thereof
US8309323B2 (en) * 2007-11-13 2012-11-13 Synthetic Genomics, Inc. Dimethyloctane as an advanced biofuel
AU2008334945C1 (en) * 2007-12-13 2013-09-05 Danisco Us Inc. Compositions and methods for producing isoprene
EP2247746A4 (en) * 2008-01-31 2012-08-01 Amyris Inc METHOD FOR MONITORING METABOLIC WAYS
ES2656790T3 (es) * 2008-03-27 2018-02-28 Genomatica, Inc. Microorganismos para la producción de ácido adípico y otros compuestos
MX2010011068A (es) * 2008-04-08 2010-11-04 Amyris Biotechnologies Inc Expresion de secuencias heterologas.
AU2009240505B2 (en) 2008-04-23 2013-09-05 Danisco Us Inc. Isoprene synthase variants for improved microbial production of isoprene
CA3101888A1 (en) * 2008-05-16 2009-11-19 REG Life Sciences, LLC Methods and compositions for producing hydrocarbons
JP2011520471A (ja) * 2008-05-23 2011-07-21 サイバス オイルズ，エルエルシー 酵母を使用したスクアレンの生成
CA2729302A1 (en) * 2008-06-30 2010-01-14 The Goodyear Tire & Rubber Company Polymers of isoprene from renewable resources
MX318543B (es) 2008-07-02 2014-03-18 Danisco Us Inc Composiciones y metodos para producir isopreno libre de hidrocarburos de c5 bajo condicion de desacoplamiento y/o intervalos de operacion seguros.
EP2337845A1 (en) * 2008-09-15 2011-06-29 Danisco US Inc. Increased isoprene production using mevalonate kinase and isoprene synthase
CA2737158A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Increased isoprene production using the archaeal lower mevalonate pathway
KR101636403B1 (ko) 2008-10-28 2016-07-05 알이지 라이프 사이언시스, 엘엘씨 지방족 알코올들을 생산하기 위한 방법들과 조성물들
AU2010238770A1 (en) 2009-04-23 2011-11-03 Danisco Us Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
CN102666837A (zh) 2009-11-23 2012-09-12 纽塞利斯公司 使用酵母产生角鲨烯的方法和组合物
CN105671108A (zh) 2010-06-02 2016-06-15 沃维公司 甜菊糖苷的重组生产
CA2816306A1 (en) 2010-10-27 2012-05-03 Danisco Us Inc. Isoprene synthase variants for improved production of isoprene
CN108396044A (zh) 2011-08-08 2018-08-14 埃沃尔瓦公司 甜菊醇糖苷类的重组生产
IN2014CN01129A (https=) 2011-08-08 2015-04-10 Evolva Sa
EP2776571B1 (en) * 2011-11-09 2017-04-12 Amyris, Inc. Production of acetyl-coenzyme a derived isoprenoids
US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene
WO2013181647A2 (en) 2012-06-01 2013-12-05 Danisco Us Inc. Compositions and methods of producing isoprene and/or industrrial bio-products using anaerobic microorganisms
JP5827601B2 (ja) * 2012-07-04 2015-12-02 住友ゴム工業株式会社 特定の蛋白質の発現を光応答転写因子により調整する方法、光応答転写因子をコードする遺伝子が導入されたイソプレノイド産生植物、及び該イソプレノイド産生植物を用いたポリイソプレノイドの製造方法
CN103087972B (zh) 2013-02-01 2014-11-05 中国科学院天津工业生物技术研究所 生产萜类化合物的重组微生物及构建方法
SG11201505597SA (en) 2013-02-06 2015-08-28 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
CA2900882A1 (en) 2013-02-11 2014-08-14 Evolva Sa Efficient production of steviol glycosides in recombinants hosts
DK2970926T3 (en) 2013-03-13 2018-04-16 Dsm Nutritional Products Ag GENMANIPULATION OF MICROorganisms
JP6282813B2 (ja) * 2013-07-16 2018-02-21 住友ゴム工業株式会社 ヒドロキシメチルグルタリルＣｏＡレダクターゼの発現をｂＺＩＰ型転写因子により調整する方法、ｂＺＩＰ型転写因子をコードする遺伝子が導入されたイソプレノイド産生植物、及び該イソプレノイド産生植物を用いたポリイソプレノイドの製造方法
CN103602626B (zh) * 2013-10-12 2016-02-10 中国科学院青岛生物能源与过程研究所 一种以油脂为原料生产甲羟戊酸的方法及其构建的基因工程菌
AU2015303294A1 (en) 2014-08-11 2017-02-02 Evolva Sa. Production of steviol glycosides in recombinant hosts
AU2015314251A1 (en) 2014-09-09 2017-03-16 Evolva Sa Production of steviol glycosides in recombinant hosts
SG11201705606PA (en) 2015-01-30 2017-08-30 Evolva Sa Production of steviol glycosides in recombinant hosts
CA2979931A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
AU2016307066A1 (en) 2015-08-07 2018-02-08 Evolva Sa Production of steviol glycosides in recombinant hosts
US10982249B2 (en) 2016-04-13 2021-04-20 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2017198682A1 (en) 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
CN106367508B (zh) * 2016-09-13 2019-10-29 东北农业大学 一种用于鉴定杂合型类异戊二烯化合物产生菌的简并引物与应用
US11396669B2 (en) 2016-11-07 2022-07-26 Evolva Sa Production of steviol glycosides in recombinant hosts
US11365417B2 (en) * 2017-09-12 2022-06-21 Bio Capital Holdings, LLC Biological devices and methods of use thereof to produce steviol glycosides
WO2019090286A2 (en) * 2017-11-06 2019-05-09 The Regents Of The University Of California Production of valerenic acid in fungal cells
CA3084263A1 (en) 2017-12-07 2019-06-13 Zymergen Inc. Engineered biosynthetic pathways for production of (6e)-8-hydroxygeraniol by fermentation
BR112020012704A8 (pt) 2017-12-21 2022-03-03 Zymergen Inc Nepetalactol oxidorredutases, nepetalactol sintases, e micróbios capazes de produzir nepetalactona
CN109777815B (zh) * 2019-03-28 2021-10-29 昆明理工大学 HMG-CoA合成酶基因RKHMGCS及其应用
CN117165502A (zh) * 2022-05-26 2023-12-05 牛津大学(苏州)科技有限公司 一种合成萜类化合物的共培养系统和方法
NL2032683B1 (en) * 2022-07-18 2024-01-26 Sestina Bio Llc Bioproduction of isoprenoids
CN120167011A (zh) * 2022-11-14 2025-06-17 新加坡科技研究局 类异戊二烯的生物合成
CN116925991B (zh) * 2023-07-28 2024-08-09 天津大学 高产甲羟戊酸的重组盐单胞菌菌株及构建方法与应用
CN120041291A (zh) * 2023-11-24 2025-05-27 牛津大学(苏州)科技有限公司 用于合成目标化合物的共培养系统和方法
WO2025217176A1 (en) * 2024-04-08 2025-10-16 Board Of Trustees Of Michigan State University Genetically engineered bacteria as a chassis host for high yield terpenoid production

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001031043A1 (en) * 1999-10-27 2001-05-03 The University Of Bristol Increasing isoprenoid biosynthesis
US20030148416A1 (en) * 2001-06-06 2003-08-07 Roche Vitamins Inc. Isoprenoid production
WO2005033287A2 (en) * 2003-09-29 2005-04-14 The Regents Of The University Of California Methods for identifying a biosynthetic pathway gene product

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5460949A (en) 1990-11-15 1995-10-24 Amoco Corporation Method and composition for increasing the accumulation of squalene and specific sterols in yeast
WO1997027317A1 (en) 1996-01-23 1997-07-31 Affymetrix, Inc. Nucleic acid analysis techniques
KR100551104B1 (ko) 1994-12-09 2006-02-09 임페리얼 컬리지 이노베이션스 리미티드 유전자의 동정
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US6080849A (en) 1997-09-10 2000-06-27 Vion Pharmaceuticals, Inc. Genetically modified tumor-targeted bacteria with reduced virulence
US6033861A (en) 1997-11-19 2000-03-07 Incyte Genetics, Inc. Methods for obtaining nucleic acid containing a mutation
JP4579415B2 (ja) 1998-07-06 2010-11-10 ディーシーブイ・インコーポレイテッド・ドゥーイング・ビジネス・アズ・バイオ−テクニカル・リソーシィズ ビタミン製造方法
US6531303B1 (en) 1998-07-06 2003-03-11 Arkion Life Sciences Llc Method of producing geranylgeraniol
CN1315947A (zh) * 1998-07-06 2001-10-03 伊斯曼化学公司 生育酚的制备
AR022383A1 (es) 1998-09-18 2002-09-04 Univ Kentucky Res Found Sintasas
US6645747B1 (en) 1999-09-21 2003-11-11 E. I. Du Pont De Nemours And Company Cis-prenyltransferases from plants
AU2001278098A1 (en) 2000-07-31 2002-02-13 Frederick M. Hahn Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
EP1409530A2 (en) 2000-09-19 2004-04-21 Microbia, Inc. Modulation of secondary metabolite production by zinc binuclear cluster proteins
JP3838033B2 (ja) 2000-12-28 2006-10-25 トヨタ自動車株式会社 プレニルアルコールの製造方法
US20040063182A1 (en) 2000-12-28 2004-04-01 Chikara Ohto Process for producing prenyl alcohol
US7238514B2 (en) 2001-01-05 2007-07-03 William Marsh Rice University Diterpene-producing unicellular organism
GB0105924D0 (en) 2001-03-09 2001-04-25 Microscience Ltd Promoter
US20040110259A1 (en) 2001-08-03 2004-06-10 Baugh Mariah R Drug metabolizing enzymes
US7172886B2 (en) 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
US7192751B2 (en) 2001-12-06 2007-03-20 The Regents Of The University Of California Biosynthesis of amorpha-4,11-diene

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001031043A1 (en) * 1999-10-27 2001-05-03 The University Of Bristol Increasing isoprenoid biosynthesis
US20030148416A1 (en) * 2001-06-06 2003-08-07 Roche Vitamins Inc. Isoprenoid production
WO2005033287A2 (en) * 2003-09-29 2005-04-14 The Regents Of The University Of California Methods for identifying a biosynthetic pathway gene product

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AU2005327292A1 (en) 2006-08-17
US7183089B2 (en) 2007-02-27
BRPI0510115A (pt) 2007-09-25
ATE537269T1 (de) 2011-12-15
US20060079476A1 (en) 2006-04-13
WO2006085899A2 (en) 2006-08-17
CA2567547C (en) 2012-10-23
BRPI0510115B1 (pt) 2022-09-27
AU2005327292B2 (en) 2010-11-04
EP1765418A2 (en) 2007-03-28
US20090004724A1 (en) 2009-01-01
WO2006085899A3 (en) 2007-05-18
EP1765418A4 (en) 2008-07-02
MXPA06013502A (es) 2007-03-01
EP1765418B1 (en) 2011-12-14
CN101023181A (zh) 2007-08-22
US7670825B2 (en) 2010-03-02
JP2008500063A (ja) 2008-01-10
CA2567547A1 (en) 2006-08-17
ES2379368T3 (es) 2012-04-25
EP2365090A1 (en) 2011-09-14

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