CA2324262C - Confocal microscopy imaging system - Google Patents
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- CA2324262C CA2324262C CA2324262A CA2324262A CA2324262C CA 2324262 C CA2324262 C CA 2324262C CA 2324262 A CA2324262 A CA 2324262A CA 2324262 A CA2324262 A CA 2324262A CA 2324262 C CA2324262 C CA 2324262C
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| CA2324262A1 CA2324262A1 (en) | 1999-09-23 |
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| US20030036855A1 (en) | 1998-03-16 | 2003-02-20 | Praelux Incorporated, A Corporation Of New Jersey | Method and apparatus for screening chemical compounds |
| GB2338568B (en) * | 1998-06-19 | 2000-12-20 | Optiscan Pty Ltd | Two photon endoscope or microscope method and apparatus |
| US6690463B2 (en) * | 2000-02-10 | 2004-02-10 | Evotec Biosystems Ag | Fluorescence intensity and lifetime distribution analysis |
| WO2002035474A1 (en) * | 2000-10-27 | 2002-05-02 | Praelux Incorporated | Method and apparatus for screening chemical compounds |
| FR2820828B1 (fr) * | 2001-02-09 | 2003-05-02 | Commissariat Energie Atomique | Dispositif d'observation d'echantillons par fluorescence, notamment de facon sequentielle |
| DE10117723A1 (de) * | 2001-04-09 | 2002-10-17 | Evotec Ag | Probenträger, insbesondere für biochemische Reaktionen |
| US7219016B2 (en) | 2001-04-20 | 2007-05-15 | Yale University | Systems and methods for automated analysis of cells and tissues |
| DE10122607B4 (de) * | 2001-05-10 | 2006-11-30 | Leica Microsystems Cms Gmbh | Verfahren und Anordnung zur direkten Fourierabbildung von Proben |
| DE10157511A1 (de) | 2001-11-23 | 2003-06-12 | Evotec Ag | Verfahren und Vorrichtung zur Korrektur der Größe und/oder Form eines Messvolumens in einer chemischen und/oder biologischen Probe |
| JP2003177131A (ja) * | 2001-12-11 | 2003-06-27 | Olympus Optical Co Ltd | 生物学的な結合親和性を検出する方法 |
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| US9157860B2 (en) | 2002-05-16 | 2015-10-13 | Applied Biosystems, Llc | Achromatic lens array |
| DE102004014048B4 (de) * | 2004-03-19 | 2008-10-30 | Sirona Dental Systems Gmbh | Vermessungseinrichtung und Verfahren nach dem Grundprinzip der konfokalen Mikroskopie |
| US7170675B2 (en) | 2004-05-19 | 2007-01-30 | Celloptic, Inc. | Method and system for wide-field multi-photon microscopy having a confocal excitation plane |
| DE102004034970A1 (de) * | 2004-07-16 | 2006-02-02 | Carl Zeiss Jena Gmbh | Lichtrastermikroskop und Verwendung |
| CA2574343C (en) * | 2004-07-23 | 2012-05-01 | Paul Donders | Method and apparatus for fluorescent confocal microscopy |
| CN1310023C (zh) * | 2004-11-10 | 2007-04-11 | 哈尔滨工业大学 | 三差动共焦显微三维超分辨成像方法 |
| GB0427050D0 (en) * | 2004-12-10 | 2005-01-12 | Amersham Biosciences Uk Ltd | Method of,and apparatus and computer software for,imaging biological objects |
| DE202005010588U1 (de) * | 2005-07-04 | 2005-10-13 | Weiss Umwelttechnik Gmbh Simulationsanlagen-Messtechnik | Anordnung zum Prüfen von pharmazeutischen Substanzen |
| US7329860B2 (en) | 2005-11-23 | 2008-02-12 | Illumina, Inc. | Confocal imaging methods and apparatus |
| KR100737170B1 (ko) * | 2006-01-20 | 2007-07-10 | 경북대학교 산학협력단 | 양자점 형광체를 이용한 이동식 형광 측정장치 |
| US7567346B2 (en) * | 2006-03-01 | 2009-07-28 | General Electric Company | System and method for multimode imaging |
| EP2032982B1 (en) | 2006-05-05 | 2012-08-29 | Yale University | Use of subcellular localization profiles as prognostic or predictive indicators |
| CA2657324A1 (en) | 2006-07-13 | 2008-01-17 | Yale University | Methods for making cancer prognoses based on subcellular localization of biomarkers |
| US7838302B2 (en) | 2006-08-07 | 2010-11-23 | President And Fellows Of Harvard College | Sub-diffraction limit image resolution and other imaging techniques |
| GB0625775D0 (en) | 2006-12-22 | 2007-02-07 | Isis Innovation | Focusing apparatus and method |
| JP4957458B2 (ja) * | 2007-08-27 | 2012-06-20 | 株式会社Jvcケンウッド | ボイスコイル及びスピーカ |
| US20100208340A1 (en) * | 2007-10-11 | 2010-08-19 | Nikon Corporation | Observation device |
| EP4060327A1 (en) | 2007-12-21 | 2022-09-21 | President and Fellows of Harvard College | Sub-diffraction limit image resolution in three dimensions |
| WO2009115108A1 (en) * | 2008-03-19 | 2009-09-24 | Ruprecht-Karls-Universität Heidelberg | A method and an apparatus for localization of single dye molecules in the fluorescent microscopy |
| KR101532611B1 (ko) * | 2009-04-28 | 2015-07-01 | 삼성전자주식회사 | 주밍샷 효과를 나타내는 디지털 영상 처리 장치 및 방법 |
| EP2543990B1 (en) * | 2010-03-01 | 2019-06-26 | Olympus Corporation | Optical analysis device, optical analysis method, and computer program for optical analysis |
| US8692708B2 (en) * | 2010-03-30 | 2014-04-08 | Sony Corporation | Radiometric imaging device and corresponding method |
| US8767069B2 (en) * | 2010-06-30 | 2014-07-01 | Luminex Corporation | Apparatus, system, and method for increasing measurement accuracy in a particle imaging device using light distribution |
| CN103026205B (zh) | 2010-07-26 | 2016-03-23 | 奥林巴斯株式会社 | 使用发光探针检测溶液中稀疏颗粒的方法 |
| EP2602612A4 (en) | 2010-09-10 | 2018-05-16 | Olympus Corporation | Optical analysis method using optical measurement in multiple wavelength bands |
| JP5856962B2 (ja) | 2010-09-10 | 2016-02-10 | オリンパス株式会社 | 単一発光粒子の光強度を用いた光分析方法 |
| CN103119421B (zh) | 2010-09-21 | 2015-04-08 | 奥林巴斯株式会社 | 利用单个发光粒子检测的光分析方法 |
| JP5737704B2 (ja) * | 2010-09-27 | 2015-06-17 | 株式会社ライフテック | 蛍光一粒子検出方法および検出システム |
| WO2012050011A1 (ja) * | 2010-10-13 | 2012-04-19 | オリンパス株式会社 | 単一発光粒子検出を用いた粒子の拡散特性値の測定方法 |
| EP2620763A4 (en) | 2010-10-19 | 2015-05-27 | Olympus Corp | OPTICAL ANALYSIS DEVICE FOR OBSERVING THE POLARIZATION CHARACTERISTICS OF A SINGLE LIGHT-EMITTING PARTICLE, OPTICAL ANALYSIS METHOD, AND OPTICAL ANALYSIS COMPUTER PROGRAM |
| EP2631631B1 (en) | 2010-11-25 | 2016-01-20 | Olympus Corporation | Photometric analysis device and photometric analysis method using wavelength characteristic of light emitted from single illuminant particle |
| JP5856983B2 (ja) | 2011-01-20 | 2016-02-10 | オリンパス株式会社 | 単一発光粒子からの光の検出を用いた光分析方法及び光分析装置 |
| JP5856984B2 (ja) | 2011-01-26 | 2016-02-10 | オリンパス株式会社 | 核酸分子の多型識別方法 |
| EP2669376B1 (en) | 2011-01-26 | 2017-08-16 | Olympus Corporation | Method for identifying polymorphism of nucleic acid molecules |
| US9477072B2 (en) * | 2011-03-01 | 2016-10-25 | Ge Healthcare Bio-Sciences Corp. | Systems and methods for illumination phase control in fluorescence microscopy |
| CN103460026B (zh) | 2011-03-29 | 2015-06-10 | 奥林巴斯株式会社 | 利用单个发光粒子检测的光分析装置、光分析方法以及光分析用计算机程序 |
| WO2012141019A1 (ja) | 2011-04-13 | 2012-10-18 | オリンパス株式会社 | 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム |
| WO2012144528A1 (ja) | 2011-04-18 | 2012-10-26 | オリンパス株式会社 | 標的粒子の定量方法、光分析装置及び光分析用コンピュータプログラム |
| EP2743682B1 (en) | 2011-08-11 | 2017-05-31 | Olympus Corporation | Method for detecting target particles |
| WO2013024650A1 (ja) | 2011-08-15 | 2013-02-21 | オリンパス株式会社 | 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム |
| CN103765196B (zh) | 2011-08-26 | 2016-03-02 | 奥林巴斯株式会社 | 利用单个发光粒子检测的光分析装置及光分析方法 |
| WO2013031309A1 (ja) | 2011-08-26 | 2013-03-07 | オリンパス株式会社 | 光分析を用いた単一粒子検出装置、単一粒子検出方法及び単一粒子検出用コンピュータプログラム |
| WO2013031365A1 (ja) | 2011-08-30 | 2013-03-07 | オリンパス株式会社 | 標的粒子の検出方法 |
| WO2013031377A1 (ja) | 2011-08-30 | 2013-03-07 | オリンパス株式会社 | 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム |
| EP2745094B1 (en) * | 2011-09-06 | 2019-05-08 | Koninklijke Philips N.V. | Optical biosensor with a plurality of sensor regions |
| EP2778658A4 (en) | 2011-11-10 | 2015-12-02 | Olympus Corp | SPECTROSCOPY DEVICE, SPECTROSCOPY PROCESS AND SPECTROSCOPY COMPUTER PROGRAM DETECTING SINGLE LIGHT-EMITTING PARTICLES |
| DE102011087196A1 (de) * | 2011-11-28 | 2013-05-29 | Leica Microsystems Cms Gmbh | Mikroskopbeleuchtungssystem und -verfahren |
| EP2816344A4 (en) | 2012-02-17 | 2015-09-23 | Olympus Corp | OPTICAL ANALYSIS DEVICE USING SINGLE PARTICLE DETECTION TECHNIQUE, OPTICAL ANALYSIS METHOD, AND COMPUTER PROGRAM FOR OPTICAL ANALYSIS |
| EP2818850B1 (en) | 2012-02-22 | 2016-08-03 | Olympus Corporation | Method for detecting a target particle |
| CH706326A2 (de) * | 2012-03-14 | 2013-09-30 | Tecan Trading Ag | Verfahren und Mikroplatten-Reader zum Untersuchung von biologischen Zellen oder Zellkulturen. |
| EP2829614A4 (en) | 2012-03-21 | 2016-03-16 | Olympus Corp | METHOD FOR DETECTING A TARGET NUCLEIC ACID MOLECULE |
| WO2013157283A1 (ja) | 2012-04-18 | 2013-10-24 | オリンパス株式会社 | 標的粒子の検出方法 |
| CN104246479B (zh) | 2012-04-18 | 2016-10-19 | 奥林巴斯株式会社 | 利用光分析的单个粒子检测装置、单个粒子检测方法以及单个粒子检测用计算机程序 |
| CN102841083B (zh) * | 2012-06-11 | 2014-08-20 | 北京大学 | 一种激光扫描位相显微成像方法及系统 |
| US8654352B1 (en) * | 2012-08-08 | 2014-02-18 | Asm Technology Singapore Pte Ltd | Chromatic confocal scanning apparatus |
| CN104155279B (zh) * | 2013-05-13 | 2017-04-26 | 中国科学院大连化学物理研究所 | 一种线形共聚焦紫外拉曼光谱仪 |
| JP6360481B2 (ja) | 2013-07-31 | 2018-07-18 | オリンパス株式会社 | 単一発光粒子検出技術を用いた光学顕微鏡装置、顕微鏡観察法及び顕微鏡観察のためのコンピュータプログラム |
| EP3059577A4 (en) | 2013-10-07 | 2017-05-31 | Olympus Corporation | Photometric analysis device employing single light-emitting particle detection, photometric analysis method, and computer program for photometric analysis |
| US10677734B2 (en) | 2014-09-05 | 2020-06-09 | Thermo Fisher Scientific Oy | Method and apparatus for optical measurement of liquid sample |
| CN104296684B (zh) * | 2014-11-05 | 2016-11-30 | 哈尔滨工业大学 | 基于表面镀膜共焦显微形貌测量装置的膜厚误差校正方法 |
| GB2534402A (en) * | 2015-01-22 | 2016-07-27 | Idea Biomedical Ltd | Auto-focussing method and device |
| CN104677884B (zh) * | 2015-03-17 | 2017-07-11 | 北京理工大学 | 高空间分辨激光分光瞳差动共焦质谱显微成像方法与装置 |
| CN104697452A (zh) * | 2015-03-24 | 2015-06-10 | 宁波高新区零零七工业设计有限公司 | 激光成像系统 |
| US9599807B2 (en) * | 2015-06-30 | 2017-03-21 | General Electric Company | Optical microscope and method for detecting lens immersion |
| WO2017098597A1 (ja) | 2015-12-09 | 2017-06-15 | オリンパス株式会社 | 単一発光粒子検出を用いた光分析方法及び光分析装置 |
| LU93022B1 (de) * | 2016-04-08 | 2017-11-08 | Leica Microsystems | Verfahren und Mikroskop zum Untersuchen einer Probe |
| CN108061964B (zh) * | 2017-10-25 | 2020-03-27 | 中国科学技术大学 | 一种可用于大样品的高速三维显微成像装置和方法 |
| CN108319008B (zh) * | 2018-02-02 | 2019-11-12 | 华中科技大学 | 一种光学显微成像方法及装置 |
| CN108680542B (zh) * | 2018-03-26 | 2020-01-10 | 华中科技大学 | 一种阵列式线扫描荧光显微成像装置 |
| CN108445619A (zh) * | 2018-05-11 | 2018-08-24 | 中国工程物理研究院流体物理研究所 | 光学扫描系统及方法 |
| CN111722388B (zh) * | 2019-03-18 | 2025-05-30 | 深圳市泽玖科技有限公司 | 一种三维微型内窥镜 |
| US20230081546A1 (en) * | 2020-01-31 | 2023-03-16 | Stellenbosch University | Method for determining mitochondrial events |
| CN111239047B (zh) * | 2020-03-09 | 2023-10-27 | 深圳中科飞测科技股份有限公司 | 一种光学设备及实现自动聚焦的方法 |
| GB2593194B (en) * | 2020-03-18 | 2022-09-07 | Refeyn Ltd | Methods and apparatus for optimised interferometric scattering microscopy |
| WO2022056385A1 (en) | 2020-09-14 | 2022-03-17 | Singular Genomics Systems, Inc. | Methods and systems for multidimensional imaging |
| KR102447224B1 (ko) * | 2020-10-26 | 2022-09-27 | 한국생산기술연구원 | 미세 입자의 정성 및 정량 분석 장치 |
| KR102766494B1 (ko) * | 2021-12-30 | 2025-02-12 | 한국광기술원 | 홀로그래픽 이미지 센서를 포함한 홀로그래픽 현미경 |
| CN114813706B (zh) * | 2022-06-29 | 2022-12-13 | 国科大杭州高等研究院 | 一种血细胞高光谱光镊捕获能量共振转移分析仪 |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63306413A (ja) * | 1987-06-09 | 1988-12-14 | Olympus Optical Co Ltd | 走査型光学顕微鏡 |
| US4844617A (en) * | 1988-01-20 | 1989-07-04 | Tencor Instruments | Confocal measuring microscope with automatic focusing |
| US5020891A (en) * | 1988-09-14 | 1991-06-04 | Washington University | Single aperture confocal scanning biomicroscope and kit for converting single lamp biomicroscope thereto |
| US5034613A (en) * | 1989-11-14 | 1991-07-23 | Cornell Research Foundation, Inc. | Two-photon laser microscopy |
| US5274240A (en) * | 1990-01-12 | 1993-12-28 | The Regents Of The University Of California | Capillary array confocal fluorescence scanner and method |
| GB9015793D0 (en) * | 1990-07-18 | 1990-09-05 | Medical Res Council | Confocal scanning optical microscope |
| JPH04221917A (ja) * | 1990-12-25 | 1992-08-12 | Tamron Co Ltd | 顕微鏡の自動焦点調節方法およびその装置 |
| US5162641A (en) * | 1991-02-19 | 1992-11-10 | Phoenix Laser Systems, Inc. | System and method for detecting, correcting and measuring depth movement of target tissue in a laser surgical system |
| US5784162A (en) * | 1993-08-18 | 1998-07-21 | Applied Spectral Imaging Ltd. | Spectral bio-imaging methods for biological research, medical diagnostics and therapy |
| US5465147A (en) * | 1991-04-29 | 1995-11-07 | Massachusetts Institute Of Technology | Method and apparatus for acquiring images using a ccd detector array and no transverse scanner |
| JPH05188301A (ja) * | 1991-09-09 | 1993-07-30 | Sumitomo Electric Ind Ltd | レーザ顕微鏡 |
| JPH05332733A (ja) * | 1992-05-27 | 1993-12-14 | Hitachi Ltd | 検出光学系並びに立体形状検出方法 |
| JPH07128596A (ja) * | 1993-09-08 | 1995-05-19 | Nikon Corp | コンフォーカル顕微鏡 |
| US5587832A (en) * | 1993-10-20 | 1996-12-24 | Biophysica Technologies, Inc. | Spatially light modulated confocal microscope and method |
| JP3450406B2 (ja) * | 1994-03-10 | 2003-09-22 | オリンパス光学工業株式会社 | 観察画像の位置調整装置及び走査型光学顕微鏡 |
| JPH07281100A (ja) * | 1994-04-05 | 1995-10-27 | Nikon Corp | オートフォーカス顕微鏡用の挟持標本体 |
| US5515864A (en) * | 1994-04-21 | 1996-05-14 | Zuckerman; Ralph | Method and apparatus for the in vivo measurement of oxygen concentration levels by the indirect determination of fluoescence lifetime |
| EP0902885A4 (en) * | 1996-05-16 | 2006-09-27 | Affymetrix Inc | SYSTEMS AND METHODS FOR DETECTION OF BRANDED PRODUCTS |
| US5900949A (en) * | 1996-05-23 | 1999-05-04 | Hewlett-Packard Company | CCD imager for confocal scanning microscopy |
| US5880465A (en) * | 1996-05-31 | 1999-03-09 | Kovex Corporation | Scanning confocal microscope with oscillating objective lens |
| US5915048A (en) * | 1996-06-05 | 1999-06-22 | Zetetic Institute | Method and apparatus for discriminating in-focus images from out-of-focus light signals from background and foreground light sources |
| JPH09329749A (ja) * | 1996-06-11 | 1997-12-22 | Nikon Corp | 光走査型顕微鏡 |
| DE19627568A1 (de) * | 1996-07-09 | 1998-01-15 | Zeiss Carl Jena Gmbh | Anordnung und Verfahren zur konfokalen Mikroskopie |
| DE19632594A1 (de) * | 1996-08-13 | 1998-02-19 | Johannes Prof Dr Schwider | Konfokales Mikroskop unter Anwendung von refraktiven Mikrolinsen-Feldern |
| US5760901A (en) * | 1997-01-28 | 1998-06-02 | Zetetic Institute | Method and apparatus for confocal interference microscopy with background amplitude reduction and compensation |
| JP3816632B2 (ja) * | 1997-05-14 | 2006-08-30 | オリンパス株式会社 | 走査型顕微鏡 |
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| KR20050088500A (ko) | 2005-09-06 |
| NO20004601L (no) | 2000-11-10 |
| NO20004601D0 (no) | 2000-09-15 |
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