WO1999047963A8 - Confocal microscopy imaging system - Google Patents

Confocal microscopy imaging system

Info

Publication number
WO1999047963A8
WO1999047963A8 PCT/US1999/005589 US9905589W WO9947963A8 WO 1999047963 A8 WO1999047963 A8 WO 1999047963A8 US 9905589 W US9905589 W US 9905589W WO 9947963 A8 WO9947963 A8 WO 9947963A8
Authority
WO
WIPO (PCT)
Prior art keywords
imaging system
confocal microscopy
microscopy imaging
ccd
directed
Prior art date
Application number
PCT/US1999/005589
Other languages
French (fr)
Other versions
WO1999047963A9 (en
WO1999047963A1 (en
Inventor
Jay K Trautman
Timothy D Harris
Richard L Hansen
William Karsh
Neal A Nicklaus
Original Assignee
Praelux Inc
Jay K Trautman
Timothy D Harris
Richard L Hansen
William Karsh
Neal A Nicklaus
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CA2324262A priority Critical patent/CA2324262C/en
Priority to AU33547/99A priority patent/AU758571B2/en
Priority to IL13849699A priority patent/IL138496A0/en
Priority to BRPI9908767-7A priority patent/BR9908767B1/en
Priority to JP2000537103A priority patent/JP4812937B2/en
Priority to EP99914908A priority patent/EP1064579A4/en
Application filed by Praelux Inc, Jay K Trautman, Timothy D Harris, Richard L Hansen, William Karsh, Neal A Nicklaus filed Critical Praelux Inc
Priority to US09/333,749 priority patent/US6388788B1/en
Publication of WO1999047963A1 publication Critical patent/WO1999047963A1/en
Publication of WO1999047963A8 publication Critical patent/WO1999047963A8/en
Publication of WO1999047963A9 publication Critical patent/WO1999047963A9/en
Priority to NO20004601A priority patent/NO20004601L/en
Priority to US09/724,253 priority patent/US6400487B1/en
Priority to US10/227,552 priority patent/US20030036855A1/en
Priority to IL16431904A priority patent/IL164319A0/en
Priority to US12/028,345 priority patent/US7957911B2/en
Priority to US13/150,532 priority patent/US20110255756A1/en

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G02B21/0084—Details of detection or image processing, including general computer control time-scale detection, e.g. strobed, ultra-fast, heterodyne detection
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0028—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders specially adapted for specific applications, e.g. for endoscopes, ophthalmoscopes, attachments to conventional microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5002—Partitioning blood components
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/006—Optical details of the image generation focusing arrangements; selection of the plane to be imaged
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0064—Optical details of the image generation multi-spectral or wavelength-selective arrangements, e.g. wavelength fan-out, chromatic profiling
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0072—Optical details of the image generation details concerning resolution or correction, including general design of CSOM objectives
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Radiology & Medical Imaging (AREA)
  • Pathology (AREA)
  • Surgery (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Ophthalmology & Optometry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A confocal imaging system utilizing an elongated beam. Specific embodiments are directed to the apparatus with charged couple devices (CCD) and those in which the apparatus is used in fluorescent object observation.
PCT/US1999/005589 1998-03-16 1999-03-16 Confocal microscopy imaging system WO1999047963A1 (en)

Priority Applications (13)

Application Number Priority Date Filing Date Title
CA2324262A CA2324262C (en) 1998-03-16 1999-03-16 Confocal microscopy imaging system
AU33547/99A AU758571B2 (en) 1998-03-16 1999-03-16 Confocal microscopy imaging system
IL13849699A IL138496A0 (en) 1998-03-16 1999-03-16 Confocal microscopy imaging system
BRPI9908767-7A BR9908767B1 (en) 1998-03-16 1999-03-16 confocal imaging system, focusing system for use with a substrate, and processes for examining an object, and focusing for use with a substrate.
JP2000537103A JP4812937B2 (en) 1998-03-16 1999-03-16 Confocal microscope imaging system
EP99914908A EP1064579A4 (en) 1998-03-16 1999-03-16 Confocal microscopy imaging system
US09/333,749 US6388788B1 (en) 1998-03-16 1999-06-15 Method and apparatus for screening chemical compounds
NO20004601A NO20004601L (en) 1998-03-16 2000-09-15 A system for confocal microscopy imaging
US09/724,253 US6400487B1 (en) 1998-03-16 2000-11-28 Method and apparatus for screening chemical compounds
US10/227,552 US20030036855A1 (en) 1998-03-16 2002-08-16 Method and apparatus for screening chemical compounds
IL16431904A IL164319A0 (en) 1998-03-16 2004-09-28 Confocal microscopy imaging system
US12/028,345 US7957911B2 (en) 1998-03-16 2008-02-08 Method and apparatus for screening chemical compounds
US13/150,532 US20110255756A1 (en) 1998-03-16 2011-06-01 Method and apparatus for screening chemical compounds

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4252798A 1998-03-16 1998-03-16
US09/042,527 1998-03-16

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US4252798A Continuation-In-Part 1998-03-16 1998-03-16

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US4252798A Continuation 1998-03-16 1998-03-16
US30033599A Continuation 1998-03-16 1999-04-27

Publications (3)

Publication Number Publication Date
WO1999047963A1 WO1999047963A1 (en) 1999-09-23
WO1999047963A8 true WO1999047963A8 (en) 1999-11-18
WO1999047963A9 WO1999047963A9 (en) 1999-12-23

Family

ID=21922413

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/005589 WO1999047963A1 (en) 1998-03-16 1999-03-16 Confocal microscopy imaging system

Country Status (10)

Country Link
EP (1) EP1064579A4 (en)
JP (1) JP4812937B2 (en)
KR (2) KR100618502B1 (en)
CN (1) CN100380160C (en)
AU (1) AU758571B2 (en)
BR (1) BR9908767B1 (en)
CA (1) CA2324262C (en)
IL (2) IL138496A0 (en)
NO (1) NO20004601L (en)
WO (1) WO1999047963A1 (en)

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EP1064579A4 (en) 2007-11-07
IL164319A0 (en) 2005-12-18
KR100560588B1 (en) 2006-03-16
KR100618502B1 (en) 2006-09-01
AU758571B2 (en) 2003-03-27
KR20050088500A (en) 2005-09-06
WO1999047963A9 (en) 1999-12-23
NO20004601L (en) 2000-11-10
CN100380160C (en) 2008-04-09
NO20004601D0 (en) 2000-09-15
WO1999047963A1 (en) 1999-09-23
BR9908767A (en) 2001-10-16
CA2324262C (en) 2010-05-25
JP4812937B2 (en) 2011-11-09
IL138496A0 (en) 2001-10-31
CN1301357A (en) 2001-06-27
KR20010041945A (en) 2001-05-25
EP1064579A1 (en) 2001-01-03
AU3354799A (en) 1999-10-11
JP2002507762A (en) 2002-03-12
BR9908767B1 (en) 2011-09-06
CA2324262A1 (en) 1999-09-23

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