BR0115558A - ácido nucleico isolado, construção de expressão recombinante, célula de levedura transformada com a construção de expressão recombinante, e, método para produzir ácido lático - Google Patents

ácido nucleico isolado, construção de expressão recombinante, célula de levedura transformada com a construção de expressão recombinante, e, método para produzir ácido lático

Info

Publication number
BR0115558A
BR0115558A BR0115558-0A BR0115558A BR0115558A BR 0115558 A BR0115558 A BR 0115558A BR 0115558 A BR0115558 A BR 0115558A BR 0115558 A BR0115558 A BR 0115558A
Authority
BR
Brazil
Prior art keywords
recombinant expression
expression construct
isolated nucleic
nucleic acid
cell transformed
Prior art date
Application number
BR0115558-0A
Other languages
English (en)
Inventor
Vineet Rajgarhia
Merja Penttila
Laura Ruohonen
Marja Ilmen
Kari Koivuranta
Original Assignee
Cargill Dow Polymers Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cargill Dow Polymers Llc filed Critical Cargill Dow Polymers Llc
Publication of BR0115558A publication Critical patent/BR0115558A/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56Lactic acid

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"áCIDO NUCLEICO ISOLADO, CONSTRUçãO DE EXPRESSãO RECOMBINANTE, CéLULA DE LEVEDURA TRANSFORMADA COM A CONSTRUçãO DE EXPRESSãO RECOMBINANTE, E, MéTODO PARA PRODUZIR áCIDO LáTICO". Esta invenção fornece biocatalisadores que são células de levedura recombinantes que compreendem vetores de expressão recombinantes que codificam genes da lactato desidrogenase heterólogos para produzir lactato.
BR0115558-0A 2000-11-22 2001-11-23 ácido nucleico isolado, construção de expressão recombinante, célula de levedura transformada com a construção de expressão recombinante, e, método para produzir ácido lático BR0115558A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25254100P 2000-11-22 2000-11-22
PCT/US2001/044041 WO2002042471A2 (en) 2000-11-22 2001-11-23 Methods and materials for the synthesis of organic products

Publications (1)

Publication Number Publication Date
BR0115558A true BR0115558A (pt) 2004-08-17

Family

ID=22956445

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0115558-0A BR0115558A (pt) 2000-11-22 2001-11-23 ácido nucleico isolado, construção de expressão recombinante, célula de levedura transformada com a construção de expressão recombinante, e, método para produzir ácido lático

Country Status (8)

Country Link
US (2) US7109010B2 (pt)
EP (1) EP1339852A2 (pt)
JP (2) JP2004521619A (pt)
CN (2) CN1955299A (pt)
AU (1) AU2002237676A1 (pt)
BR (1) BR0115558A (pt)
CA (1) CA2430270A1 (pt)
WO (1) WO2002042471A2 (pt)

Families Citing this family (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1294728B1 (it) 1997-09-12 1999-04-12 Biopolo S C A R L Ceppi di lievito per la riproduzione di acido lattico
US20070031950A1 (en) * 1998-09-11 2007-02-08 Winkler Aaron A Production of D-lactic acid with yeast
CA2374482C (en) * 1999-05-21 2012-09-18 Cargill Dow Llc Methods and materials for the synthesis of organic products
US7141410B2 (en) * 2000-11-22 2006-11-28 Natureworks Llc Methods and materials for the production of organic products in cells of Candida species
WO2002101038A1 (fr) * 2001-05-29 2002-12-19 Asahi Glass Company, Limited Procede de construction d'un hote et procede de production d'une proteine etrangere
WO2003049525A2 (en) * 2001-11-23 2003-06-19 Cargill Dow Llc Methods and materials for the production of organic products in cells of $i(candida) species
BR0311452A (pt) * 2002-05-30 2005-03-29 Cargill Dow Llc Processos e materiais para a produção de ácido lático em levedura
US7405068B2 (en) 2003-05-02 2008-07-29 Tate & Lyle Ingredients Americas, Inc. Pyruvate producing yeast strain
EP1626979B1 (en) 2003-05-02 2012-06-06 Cargill, Incorporated Genetically modified yeast species and fermentation processes using genetically modified yeast
PL1756292T3 (pl) 2004-03-31 2015-03-31 Cargill Inc Sposób fermentacji cukrów zawierających oligomeryczne sacharydy
CA2568689A1 (en) * 2004-06-04 2005-12-15 Fluxome Sciences A/S Metabolically engineered cells for the production of polyunsaturated fatty acids
JP2006296377A (ja) * 2005-04-25 2006-11-02 Toyota Central Res & Dev Lab Inc 有機酸生産用形質転換体
AU2006291566A1 (en) 2005-06-02 2007-03-22 Cargill, Inc. Genetically modified yeast of the species issatchenkia orientalis and closely related species and fermentation processes using same
EP1926822B1 (en) 2005-09-19 2010-06-30 Basf Se Biocatalytic manufacturing of (meth)acrylic esters
CA2623751A1 (en) 2005-09-22 2007-04-05 Tate & Lyle Ingredients Americas, Inc. Improved strains for the production of organic acids
EP2147976B1 (en) * 2005-10-14 2014-07-23 Toray Industries, Inc. Yeast and method of producing L-lactic acid
WO2007117282A2 (en) 2005-11-23 2007-10-18 Natureworks Llc Lactic acid-producing yeast cells having nonfunctional l-or d-lactate: ferricytochrome c oxidoreductase gene
JP5622359B2 (ja) 2006-02-10 2014-11-12 デユポン・テイト・アンド・ライル・バイオ・プロダクツ・カンパニー・エルエルシー バイオマス誘導された抽出物、フレグランス濃縮物、およびオイル用の天然非刺激性溶剤としての生物誘導された1,3−プロパンジオールおよびその共役エステル
US20070275139A1 (en) * 2006-02-10 2007-11-29 Melissa Joerger Food compositions comprising renewably-based, biodegradable1,3-propanediol
KR100725021B1 (ko) * 2006-02-16 2007-06-07 주식회사 마크로젠 일차 대사산물의 대량 생산 방법, 대량 생산 균주 및 이의제조 방법
CA2645361A1 (en) 2006-03-13 2007-09-20 Cargill Inc. Yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol
AP2724A (en) 2006-07-21 2013-08-31 Xyleco Inc Conversion systems for biomass
WO2008061187A1 (en) * 2006-11-15 2008-05-22 Dupont Tate & Lyle Bio Products Company, Llc Preservative compositions comprising renewably-based, biodegradable 1,3-propanediol
CN101743310A (zh) 2006-12-07 2010-06-16 麻省理工学院 全局转录机器的改造
US20100062505A1 (en) * 2006-12-21 2010-03-11 Gevo, Inc. Butanol production by metabolically engineered yeast
US8673601B2 (en) * 2007-01-22 2014-03-18 Genomatica, Inc. Methods and organisms for growth-coupled production of 3-hydroxypropionic acid
WO2008130603A2 (en) * 2007-04-16 2008-10-30 Archer-Daniels-Midland Company Increased ethanol production from xylose
EP2155885A4 (en) * 2007-05-18 2010-12-01 Microbia Prec Engineering Inc ACIDIC ACID PRODUCTION IN RECOMBINANT YEAST
TWI525195B (zh) * 2007-08-10 2016-03-11 奇諾麥提卡公司 合成烯酸及其衍生物之方法
JP5532919B2 (ja) * 2007-12-07 2014-06-25 東レ株式会社 乳酸脱水素酵素発現カセット、形質転換酵母および乳酸の製造方法
US8455239B2 (en) * 2007-12-23 2013-06-04 Gevo, Inc. Yeast organism producing isobutanol at a high yield
EP2235193B1 (en) * 2007-12-23 2015-11-25 GEVO, Inc. Yeast organism producing isobutanol at a high yield
EP2238098A4 (en) 2007-12-27 2016-06-01 Gevo Inc RECOVERING HIGHER ALCOHOLS IN DILUTED AQUEOUS SOLUTIONS
WO2009094485A1 (en) 2008-01-22 2009-07-30 Genomatica, Inc. Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol
DK2262901T3 (en) 2008-03-05 2019-01-21 Genomatica Inc ORGANISMS PRODUCING PRIMARY ALCOHOL
CN103555643B (zh) 2008-03-27 2016-08-10 基因组股份公司 用于产生己二酸和其他化合物的微生物
AU2009242615A1 (en) * 2008-05-01 2009-11-05 Genomatica, Inc. Microorganisms for the production of methacrylic acid
BRPI0909989A2 (pt) * 2008-06-05 2021-06-22 Butamax Advanced Biofuels Llc célula de levedura recombinante e método para a produção de um produto
US8129154B2 (en) * 2008-06-17 2012-03-06 Genomatica, Inc. Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate
EP2316961A4 (en) * 2008-06-30 2012-04-25 Toyota Motor Co Ltd PROCESS FOR PREPARING ORGANIC ACID
US20100021978A1 (en) * 2008-07-23 2010-01-28 Genomatica, Inc. Methods and organisms for production of 3-hydroxypropionic acid
WO2010095751A1 (ja) * 2009-02-23 2010-08-26 キリンホールディングス株式会社 キャンディダ・ユティリスによる高効率乳酸製造法
ES2521675T3 (es) * 2008-09-29 2014-11-13 Butamax Advanced Biofuels Llc Identificación y uso de [2Fe-2S]-dihidroxi-ácido deshidratasas bacterianas
US20100184173A1 (en) * 2008-11-14 2010-07-22 Genomatica, Inc. Microorganisms for the production of methyl ethyl ketone and 2-butanol
AU2009327490A1 (en) * 2008-12-16 2011-07-28 Genomatica, Inc. Microorganisms and methods for conversion of syngas and other carbon sources to useful products
CA2761877A1 (en) * 2009-02-23 2010-08-26 Kirin Holdings Kabushiki Kaisha Method for production of substance in candida utilis using xylose as carbon source
FI20095278A0 (fi) * 2009-03-18 2009-03-18 Valtion Teknillinen Ksylonihapon valmistus
EP3318626B1 (en) * 2009-04-30 2020-01-15 Genomatica, Inc. Organisms for the production of 1,3-butanediol
BRPI1013505A2 (pt) 2009-04-30 2018-02-14 Genomatica Inc organismos para a produção de isopropanol, n-butanol, e isobutanol
KR101930540B1 (ko) 2009-05-07 2019-03-15 게노마티카 인코포레이티드 아디페이트, 헥사메틸렌디아민 및 6-아미노카프로산의 생합성을 위한 미생물 및 방법
WO2010132845A1 (en) * 2009-05-15 2010-11-18 Genomatica, Inc. Organisms for the production of cyclohexanone
WO2010144746A2 (en) * 2009-06-10 2010-12-16 Genomatica, Inc. Microorganisms and methods for carbon-efficient biosynthesis of mek and 2-butanol
EP2462221B1 (en) 2009-08-05 2017-02-22 Genomatica, Inc. Semi-synthetic terephthalic acid via microorganisms that produce muconic acid
KR20120068021A (ko) 2009-09-09 2012-06-26 게노마티카 인코포레이티드 아이소프로판올과 1차 알콜, 다이올 및 산과의 공동 생산을 위한 미생물 및 방법
DE102009029651A1 (de) * 2009-09-22 2011-03-24 Evonik Röhm Gmbh Verfahren zur Herstellung freier Carbonsäuren
JP2013507145A (ja) 2009-10-13 2013-03-04 ゲノマチカ, インク. 1,4−ブタンジオール、4−ヒドロキシブタナール、4−ヒドロキシブチリル−CoA、プトレシン及び関連化合物の生成のための微生物体並びに関連する方法
EP2491125A4 (en) * 2009-10-23 2014-04-23 Genomatica Inc MICROORGANISMS FOR ANILINE PRODUCTION
KR102071834B1 (ko) 2009-10-26 2020-01-30 에프. 호프만-라 로슈 아게 글리코실화된 면역글로불린의 제조 방법
CN109136161A (zh) * 2009-12-10 2019-01-04 基因组股份公司 合成气或其他气态碳源和甲醇转化为1,3-丁二醇的方法和有机体
SG182686A1 (en) 2010-01-29 2012-08-30 Genomatica Inc Microorganisms and methods for the biosynthesis of p-toluate and terephthalate
JP5950830B2 (ja) 2010-02-17 2016-07-13 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー Fe−Sクラスター要求タンパク質の活性の改善
US8048661B2 (en) * 2010-02-23 2011-11-01 Genomatica, Inc. Microbial organisms comprising exogenous nucleic acids encoding reductive TCA pathway enzymes
US8445244B2 (en) * 2010-02-23 2013-05-21 Genomatica, Inc. Methods for increasing product yields
US9023636B2 (en) 2010-04-30 2015-05-05 Genomatica, Inc. Microorganisms and methods for the biosynthesis of propylene
BR112012028290B1 (pt) * 2010-05-05 2021-02-02 Lallemand Hungary Liquidity Management Llc. levedura recombinante, processo para converter biomassa em etanol e meio de fermentação compreendendo dita levedura
CN103080324B (zh) 2010-05-05 2019-03-08 基因组股份公司 用于丁二烯生物合成的微生物和方法
BR112013001635A2 (pt) 2010-07-26 2016-05-24 Genomatica Inc micro-organismo e métodos para a biossíntese de aromáticos, 2, 4-pentadienoato e 1,3-butadieno
CA2818499A1 (en) 2010-11-22 2012-06-07 Novozymes, Inc. Compositions and methods for 3-hydroxypropionic acid production
WO2012071470A2 (en) 2010-11-22 2012-05-31 Cargill, Incorporated Compositions and methods for increased ethanol titer from biomass
WO2012103261A2 (en) 2011-01-25 2012-08-02 Finley Kenneth R Compositions and methods for succinate production
EP2697252B1 (en) 2011-04-11 2016-08-10 Cargill, Incorporated Compositions and methods for increased ethanol production from biomass
US9150835B2 (en) 2011-06-24 2015-10-06 Samsung Electronics Co., Ltd. Modified microorganism for highly efficient production of lactic acid
KR20130000883A (ko) * 2011-06-24 2013-01-03 삼성전자주식회사 클루이베로마이세스 마르시아누스 내에서의 향상된 단백질 생산
KR101576186B1 (ko) * 2012-06-26 2015-12-10 한국생명공학연구원 에탄올 생산 경로가 봉쇄된 클루이베로마이세스 막시아누스 균주 및 이의 용도
DE102012212281B3 (de) 2012-07-13 2013-10-31 Schülke & Mayr GmbH Mischung von natürlichen bzw. naturidentischen Alkoholen mit verbesserter Wirksamkeit
JP5890905B2 (ja) * 2012-07-23 2016-03-22 旭硝子株式会社 有機酸の製造方法
EP2877568B1 (en) 2012-07-25 2021-04-28 Cargill, Incorporated Yeast cells having reductive tca pathway from pyruvate to succinate and overexpressing an exogenous nad(p)+ transhydrogenase enzyme
WO2014076232A2 (en) 2012-11-19 2014-05-22 Novozymes A/S Isopropanol production by recombinant hosts using an hmg-coa intermediate
BR112015012409A2 (pt) 2012-11-30 2017-09-12 Novozymes Inc célula recombinante de levedura, e, método de produção de 3-hp
BR112015012946B1 (pt) 2012-12-10 2021-09-28 Cargill, Incorporated Processo de fermentação em lote para fermentar um hidrolisato de amido
US9650624B2 (en) 2012-12-28 2017-05-16 Butamax Advanced Biofuels Llc DHAD variants for butanol production
WO2014151190A1 (en) 2013-03-15 2014-09-25 Butamax Advanced Biofuels Llc Dhad variants and methods of screening
TW201437367A (zh) 2013-03-29 2014-10-01 Far Eastern New Century Corp 可利用五碳糖及六碳糖生產乳酸之酵母菌
WO2015017721A1 (en) 2013-07-31 2015-02-05 Novozymes A/S 3-hydroxypropionic acid production by recombinant yeasts expressing an insect aspartate 1-decarboxylase
KR101577134B1 (ko) * 2014-05-09 2015-12-14 씨제이제일제당 (주) 젖산 생산이 향상된 미생물 및 이를 이용하여 젖산을 생산하는 방법
US10214754B2 (en) * 2014-10-10 2019-02-26 Jmtc Enzyme Corporation Transformant and its production process, and method for producing lactic acid
BR112017020474A2 (pt) 2015-03-27 2018-07-03 Cargill Inc levedura modificada, meio de fermentação, método para produção de um bioproduto, ácido nucleico, vetor e método de fermentação para produzir etanol
JP2018526969A (ja) 2015-06-04 2018-09-20 バイオアンバー インコーポレイテッドBioamber Inc. 官能基化アルファ置換アクリレートおよびc4ジカルボキシレートのバイオベース産生
KR101704212B1 (ko) * 2015-06-12 2017-02-08 씨제이제일제당 (주) 젖산을 생산하는 미생물 및 이를 이용한 젖산 제조 방법
US9968531B2 (en) 2015-08-05 2018-05-15 Dupont Tate & Lyle Bio Products Company, Llc Deodorants containing 1,3-propanediol
AU2016301365A1 (en) 2015-08-05 2018-03-08 Cargill, Incorporated Xylose isomerase-modified yeast strains and methods for bioproduct production
WO2017035270A1 (en) 2015-08-24 2017-03-02 Novozymes A/S Beta-alanine aminotransferases for the production of 3-hydroxypropionic acid
EP3380626B1 (en) 2015-11-24 2020-10-21 Cargill, Incorporated Genetically modified yeasts and fermentation processes using genetically modified yeasts
CN109689864B (zh) 2016-07-13 2023-04-04 帝斯曼知识产权资产管理有限公司 苹果酸脱氢酶
JP6994821B2 (ja) * 2016-08-02 2022-01-14 三菱商事ライフサイエンス株式会社 サッカロミセス・セレビシエの連続培養におけるエタノール生産の低減
EP3494129A1 (en) 2016-08-05 2019-06-12 Cargill, Incorporated Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production
CN109906270A (zh) * 2016-09-16 2019-06-18 嘉吉公司 经遗传修饰的乳酸消耗酵母以及使用此类经遗传修饰的酵母的发酵工艺
JP2020506702A (ja) 2017-02-02 2020-03-05 カーギル インコーポレイテッド C6−c10脂肪酸誘導体を生成する遺伝子組み換え細胞
US11718820B2 (en) 2017-08-17 2023-08-08 Cargill, Incorporated Genetically modified haploid Issatchenkia orientalis
FI20195716A1 (en) * 2019-08-30 2021-03-01 Finnfoam Oy Genetically modified fungi and related methods and uses

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL39710A (en) 1972-06-19 1975-04-25 Imi Inst For Res & Dev Recovery of acids from aqueous solutions by solvent extraction
JP2608540B2 (ja) 1982-05-19 1997-05-07 ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ クルイベロミセス種のためのクローニング系
US4943529A (en) 1982-05-19 1990-07-24 Gist-Brocades Nv Kluyveromyces as a host strain
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5210296A (en) 1990-11-19 1993-05-11 E. I. Du Pont De Nemours And Company Recovery of lactate esters and lactic acid from fermentation broth
AT398982B (de) 1993-02-18 1995-02-27 Vogelbusch Gmbh Verfahren zur abtrennung und reinigung von milchsäure
US5510526A (en) 1993-06-29 1996-04-23 Cargill, Incorporated Lactic acid production, separation and/or recovery process
IL109003A (en) 1994-03-16 1999-09-22 Yissum Res Dev Co Process and extractant composition for extracting water-soluble carboxylic and mineral acids
CA2374482C (en) * 1999-05-21 2012-09-18 Cargill Dow Llc Methods and materials for the synthesis of organic products

Also Published As

Publication number Publication date
EP1339852A2 (en) 2003-09-03
JP2004521619A (ja) 2004-07-22
US7109010B2 (en) 2006-09-19
AU2002237676A1 (en) 2002-06-03
CA2430270A1 (en) 2002-05-30
US20030166179A1 (en) 2003-09-04
WO2002042471A3 (en) 2003-03-13
CN1486366A (zh) 2004-03-31
CN1249239C (zh) 2006-04-05
JP2007111054A (ja) 2007-05-10
WO2002042471A2 (en) 2002-05-30
US20060110810A1 (en) 2006-05-25
CN1955299A (zh) 2007-05-02

Similar Documents

Publication Publication Date Title
BR0115558A (pt) ácido nucleico isolado, construção de expressão recombinante, célula de levedura transformada com a construção de expressão recombinante, e, método para produzir ácido lático
BR0214366A (pt) construção de ácido nucleico recombinante, célula e, métodos para produzir ácido láctico, e para reduzir a atividade de piruvato descarboxilase em uma célula
BR0012712A (pt) Métodos de produção de l-aminoácidos aumentando-se o nadph celular
BRPI0814781A8 (pt) ''proteína, sequência de ácido nucleico, método para a produção de ácido itacônico, vetor, célula hospedeira, e, uso de um ácido cis-aconítico descarboxilase''
Kunze et al. Targeting of malate synthase 1 to the peroxisomes of Saccharomyces cerevisiae cells depends on growth on oleic acid medium
BR9815361A (pt) Método para procução de glicerol a partir de um organismo recombinante, célula hospedeira transformada e método para produção de 1-3-propano diol a partir de um organismo recombinante
BRPI0409954A (pt) espécies leveduras geneticamente modificados e processos de fermentação utilizando levedura geneticamente modificada
WO2004099387A3 (en) siRNA INDUCED SYSTEMIC GENE SILENCING IN MAMMALIAN SYSTEMS
EP1959011A3 (en) High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences
WO2000032757A3 (en) Plant vitamin e biosynthetic enzymes
WO2003102201A3 (en) Methods and materials for the production of d-lactic acid in yeast
ATE332388T1 (de) Methoden zur modifikation eukaryotischer zellen
BRPI0416220A (pt) método para produzir ácido láctico, cepa de levedura e s.cerevisiae tolerantes à acidez, cepa de levedura independente de fonte de carbono e tolerante à acidez, meio de cultura, cepa de levedura recombinante cepa de levedura independente de fonte de carbono c2, caldo de fermentação e plasmìdeo de levedura
MXPA03010433A (es) Metodos para produccion recombinante de peptidos antifusogenicos.
BR0003943A (pt) Sequências de nucleotìdeo que codificam para o gene thre e processo para a produção enzimática de l-treonina usando bactérias corineforma
EP1841871A4 (en) NEW L-LYSINE INDUCIBLE PROMOTER
Rother et al. Genetic and proteomic analyses of CO utilization by Methanosarcina acetivorans
DK1585822T3 (da) Promotorvarianter til ekspression af gener i en fungal celle
MX2010010309A (es) Ceculas hospederas y metodos para producir proteinas que contienen enlace de disulfuro.
PL312828A1 (en) Nucleic acid sequence for encoding a protein a. thaliana of delta-7 activity of delta-5,7-sterole reductase, method of cloning such nucleic acid, delta-7red protein, method of obtaining such protein, antibody, expression vector, transformed yeast strain, transformed host cells as well as application methods
WO2002000871A3 (de) Verfahren zur einbringung von nukleinsäuren und anderen biologisch aktiven molekülen in der kern höherer eukaryontischer zellen mit hilfe elektrischen stroms
WO1999049058A3 (en) Tryptophan biosynthetic enzymes
WO2000004154A3 (en) Genes encoding sulfate assimilation proteins
Eddy et al. Segmental message stabilization as a mechanism for differential expression from the Zymomonas mobilis gap operon
EP1728859A4 (en) SEQUENCE CAPABLE OF ACCELERATING GENE EXPRESSION AT A MODERATELY LOW TEMPERATURE

Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 7A E 8A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 DA RPI 2021 DE 29/09/2009.