AU2009235368B2 - Production of nucleic acid - Google Patents

Production of nucleic acid Download PDF

Info

Publication number
AU2009235368B2
AU2009235368B2 AU2009235368A AU2009235368A AU2009235368B2 AU 2009235368 B2 AU2009235368 B2 AU 2009235368B2 AU 2009235368 A AU2009235368 A AU 2009235368A AU 2009235368 A AU2009235368 A AU 2009235368A AU 2009235368 B2 AU2009235368 B2 AU 2009235368B2
Authority
AU
Australia
Prior art keywords
sequences
amino acids
mutations
mrna
reverse transcriptase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2009235368A
Other languages
English (en)
Other versions
AU2009235368A1 (en
Inventor
Arvydas Janulaitis
Dangira Siksniene
Remigijus Skirgaila
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Thermo Fisher Scientific Baltics UAB
Original Assignee
Thermo Fisher Scientific Baltics UAB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Thermo Fisher Scientific Baltics UAB filed Critical Thermo Fisher Scientific Baltics UAB
Publication of AU2009235368A1 publication Critical patent/AU2009235368A1/en
Application granted granted Critical
Publication of AU2009235368B2 publication Critical patent/AU2009235368B2/en
Assigned to THERMO FISHER SCIENTIFIC BALTICS UAB reassignment THERMO FISHER SCIENTIFIC BALTICS UAB Amend patent request/document other than specification (104) Assignors: FERMENTAS UAB
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/162—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2009235368A 2008-04-10 2009-04-09 Production of nucleic acid Ceased AU2009235368B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0806562.5 2008-04-10
GBGB0806562.5A GB0806562D0 (en) 2008-04-10 2008-04-10 Production of nucleic acid
PCT/EP2009/054329 WO2009125006A2 (en) 2008-04-10 2009-04-09 Production of nucleic acid

Publications (2)

Publication Number Publication Date
AU2009235368A1 AU2009235368A1 (en) 2009-10-15
AU2009235368B2 true AU2009235368B2 (en) 2014-01-16

Family

ID=39433452

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2009235368A Ceased AU2009235368B2 (en) 2008-04-10 2009-04-09 Production of nucleic acid

Country Status (14)

Country Link
US (5) US8835148B2 (enExample)
EP (4) EP3375870B1 (enExample)
JP (5) JP5642662B2 (enExample)
KR (4) KR102082768B1 (enExample)
CN (2) CN107058258A (enExample)
AU (1) AU2009235368B2 (enExample)
CA (1) CA2721117A1 (enExample)
ES (1) ES2647272T3 (enExample)
GB (1) GB0806562D0 (enExample)
IL (2) IL208578A (enExample)
NZ (1) NZ588468A (enExample)
PL (1) PL2281035T3 (enExample)
SG (1) SG10201503327QA (enExample)
WO (1) WO2009125006A2 (enExample)

Families Citing this family (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9771565B2 (en) * 2000-05-26 2017-09-26 Life Technologies Corporation Thermostable reverse transcriptases and uses thereof
EP2325303B1 (en) 2002-09-13 2013-11-06 Life Technologies Corporation Thermostable reverse transcriptases and uses thereof
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
GB0806562D0 (en) * 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
EP2957641B1 (en) * 2009-10-15 2017-05-17 Ibis Biosciences, Inc. Multiple displacement amplification
EP2604688B1 (en) * 2010-08-13 2018-01-10 Kyoto University Variant reverse transcriptase
KR101818126B1 (ko) 2011-02-09 2018-01-15 (주)바이오니아 열안정성이 증가된 역전사효소
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9745571B2 (en) * 2013-03-07 2017-08-29 Bio-Rad Laboratories, Inc. Repetitive reverse transcription partition assay
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
WO2015075842A1 (ja) * 2013-11-25 2015-05-28 国立大学法人京都大学 変異型逆転写酵素
US20150166984A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
US9663770B2 (en) 2014-01-22 2017-05-30 Life Technologies Corporation Reverse transcriptases for use in high temperature nucleic acid synthesis
CN106414704B (zh) 2014-03-14 2020-11-03 生命技术公司 用于核酸扩增和检测的集成系统
CN104250642A (zh) * 2014-04-30 2014-12-31 厦门安普利生物工程有限公司 一种提取纯化m-mlv逆转录酶的方法
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
JP2018518157A (ja) 2015-05-11 2018-07-12 イラミーナ インコーポレーテッド 治療薬剤の発見および分析のためのプラットフォーム
SG10202104041PA (en) 2015-10-23 2021-06-29 Harvard College Nucleobase editors and uses thereof
US11046940B2 (en) 2016-04-12 2021-06-29 Solis Biodyne Ou Synthetic reverse transcriptases and uses thereof
KR20250103795A (ko) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US9580698B1 (en) * 2016-09-23 2017-02-28 New England Biolabs, Inc. Mutant reverse transcriptase
SG11201903089RA (en) 2016-10-14 2019-05-30 Harvard College Aav delivery of nucleobase editors
JP7061078B2 (ja) * 2016-12-14 2022-04-27 タカラバイオ株式会社 耐熱性の逆転写酵素変異体
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
EP3592381A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Cancer vaccine
WO2018165629A1 (en) 2017-03-10 2018-09-13 President And Fellows Of Harvard College Cytosine to guanine base editor
WO2018176009A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
JP6928668B2 (ja) * 2017-04-11 2021-09-01 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した熱安定性を有する変異型逆転写酵素、ならびにそれに関する生成物、方法および使用
EP4375376A3 (en) 2017-04-26 2024-08-14 10X Genomics, Inc. Mmlv reverse transcriptase variants
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
US11649442B2 (en) 2017-09-08 2023-05-16 The Regents Of The University Of California RNA-guided endonuclease fusion polypeptides and methods of use thereof
US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
EP3728633A1 (en) 2017-12-22 2020-10-28 Thermo Fisher Scientific Baltics Uab Polymerase chain reaction composition comprising amines
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
EP3820495A4 (en) 2018-07-09 2022-07-20 The Broad Institute Inc. RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20220411783A1 (en) * 2018-10-12 2022-12-29 The Broad Institute, Inc. Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
WO2020093322A1 (zh) * 2018-11-08 2020-05-14 深圳华大生命科学研究院 一种微流控芯片及其制备方法和dna合成方法
CN113785053B (zh) * 2018-12-26 2024-07-12 深圳华大生命科学研究院 酶活性提高的逆转录酶及其应用
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
EP3942043A2 (en) 2019-03-19 2022-01-26 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US20220290112A1 (en) * 2019-07-26 2022-09-15 Toyobo Co., Ltd. Variant reverse transcriptase exhibiting excellent stability
AU2020361681A1 (en) 2019-10-10 2022-05-05 1859, Inc. Methods and systems for microfluidic screening
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
CN112795546B (zh) * 2019-11-13 2024-02-27 广州达安基因股份有限公司 一种具有高逆转录效率的耐高温逆转录酶突变体及其应用
CN112795549B (zh) * 2019-11-13 2023-11-28 广州达安基因股份有限公司 一种逆转录酶突变体
CN112795547B (zh) * 2019-11-13 2024-05-31 广州达安基因股份有限公司 高逆转录效率的逆转录酶突变体
CN112795548A (zh) * 2019-11-13 2021-05-14 中山大学达安基因股份有限公司 具有高逆转录效率的耐高温逆转录酶突变体及其应用
CN112795551B (zh) * 2019-11-13 2024-01-30 广州达安基因股份有限公司 一种耐高温逆转录酶突变体及其应用
EP4073241A2 (en) 2019-12-11 2022-10-19 10X Genomics, Inc. Reverse transcriptase variants
WO2021165508A1 (en) 2020-02-21 2021-08-26 Biogemma Prime editing technology for plant genome engineering
AU2021236683A1 (en) * 2020-03-19 2022-11-17 Intellia Therapeutics, Inc. Methods and compositions for directed genome editing
BR112022022603A2 (pt) 2020-05-08 2023-01-17 Broad Inst Inc Métodos e composições para edição simultânea de ambas as fitas de sequência alvo de nucleotídeos de fita dupla
CN111849938B (zh) * 2020-06-05 2022-02-15 广州英赞生物科技有限公司 一种突变型逆转录酶及其制备方法与应用
CN111647576B (zh) * 2020-06-24 2021-02-09 南京诺唯赞生物科技股份有限公司 一种热稳定性逆转录酶突变体及其应用
US12559734B2 (en) 2020-07-20 2026-02-24 Integrated Dna Technologies, Inc. Reverse transcriptase mutants with increased activity and thermostability
WO2022020371A2 (en) * 2020-07-20 2022-01-27 Integrated Dna Technologies, Inc. Reverse transcriptase mutants with increased activity and thermostability
CN114480336B (zh) * 2020-11-13 2023-10-20 广州达安基因股份有限公司 含有逆转录酶突变体的核酸检测试剂盒
CN114480330B (zh) * 2020-11-13 2024-11-01 广州达安基因股份有限公司 多突变位点的逆转录酶突变体
CN114480332B (zh) * 2020-11-13 2023-12-05 广州达安基因股份有限公司 耐高温m-mlv酶突变体及其应用
CN114480335B (zh) * 2020-11-13 2024-06-07 广州达安基因股份有限公司 逆转录酶及逆转录检测试剂
CN114480331A (zh) * 2020-11-13 2022-05-13 广州达安基因股份有限公司 Mmlv酶组合突变体
US20240002818A1 (en) * 2020-11-24 2024-01-04 Saliogen Therapeutics, Inc. Mammalian mobile element compositions, systems and therapeutic applications
CN112481234B (zh) * 2020-12-03 2023-09-22 重庆工业职业技术学院 一种焦磷酸酶突变体合成核酸糖类似物及其应用
CN112251423B (zh) * 2020-12-08 2021-03-19 北京健为医学检验实验室有限公司 一种m-mlv逆转录酶体及其应用
CN113234858A (zh) * 2021-05-20 2021-08-10 阿吉安(福州)基因医学检验实验室有限公司 一种covid-19的检测试剂盒
CN113174381B (zh) * 2021-06-08 2023-05-12 翌圣生物科技(上海)股份有限公司 增强型mmlv逆转录酶突变体及其应用
CN118401656A (zh) * 2021-12-17 2024-07-26 东洋纺株式会社 热稳定性优异的逆转录酶
CN114214299A (zh) * 2022-01-26 2022-03-22 苏州译酶生物科技有限公司 一种m-mlv逆转录酶及其制备方法
US11639499B1 (en) 2022-03-08 2023-05-02 Watchmaker Genomics, Inc. Reverse transcriptase variants
CN114657165B (zh) * 2022-03-25 2023-04-07 江苏百时美生物科技有限公司 一种提高热稳定性和抑制剂耐受性的逆转录酶突变体及其应用
CN114591930B (zh) * 2022-05-10 2022-08-12 翌圣生物科技(上海)股份有限公司 增强型逆转录酶、编码dna、试剂盒及其在rna文库构建中的应用
CN117106746B (zh) * 2022-06-01 2024-11-22 北京擎科生物科技股份有限公司 逆转录突变体及其应用
CN116064460B (zh) * 2022-08-01 2023-09-26 南京诺唯赞生物科技股份有限公司 一种mmlv突变体及其应用
CN117586983A (zh) * 2022-08-11 2024-02-23 菲鹏生物股份有限公司 一种逆转录酶、核酸分子及合成cDNA的方法
CN115747186B (zh) * 2022-09-13 2025-05-27 天罗诊断科技江苏有限公司 一种耐热的高效率逆转录酶突变体及其应用
CN116042569B (zh) * 2023-02-01 2023-08-15 珠海宝锐生物科技有限公司 Mmlv逆转录酶突变体及其应用
CN116004568B (zh) * 2023-02-07 2025-10-17 北京诺派生物科技有限公司 一种可用于核酸检测的耐高温反转录酶及其制备方法与应用
WO2024211850A1 (en) * 2023-04-05 2024-10-10 Primrose Bio, Inc. Methods and compositions for protein engineering
CN116694598B (zh) * 2023-06-30 2024-12-13 翌圣生物科技(上海)股份有限公司 一种鼠白血病病毒逆转录酶突变体及其应用
CN118345060A (zh) * 2023-06-30 2024-07-16 翌圣生物科技(上海)股份有限公司 一种鼠白血病病毒逆转录酶突变体及其应用
CN116732141B (zh) * 2023-07-10 2024-04-02 海南大学 一种快速检测生物dna特异性的方法
CN117025724A (zh) * 2023-08-23 2023-11-10 翌圣生物科技(上海)股份有限公司 Rna链特异性建库试剂盒及建库方法
CN117925558B (zh) * 2023-12-13 2025-03-25 郑州玛特瑞斯生物科技有限公司 突变型逆转录酶及其应用
CN118406670B (zh) * 2024-05-06 2025-06-27 湖南艾科瑞生物工程有限公司 一种mmlv逆转录酶突变体及其应用
WO2026003215A1 (fr) 2024-06-27 2026-01-02 Commissariat A L'energie Atomique Et Aux Energies Alternatives Bases modifiées chimiquement avec un groupement clivable et méthodes de sélection d'acides nucléiques fonctionnels les comprenant
CN119955759B (zh) * 2025-01-07 2025-08-08 湖南艾科瑞生物工程有限公司 一种mmlv逆转录酶突变体及其应用
CN121249621B (zh) * 2025-12-08 2026-03-31 翌圣生物科技(上海)股份有限公司 一种mmlv逆转录酶突变体及其制备方法、产品和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007022045A2 (en) * 2005-08-10 2007-02-22 Stratagene California Mutant reverse transcriptase and methods of use
US20070141592A1 (en) * 2000-05-26 2007-06-21 Smith Michael D Thermostable reverse transcriptases and uses thereof

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6236563A (ja) 1985-08-12 1987-02-17 Koji Ando 可動導体型計器
US5244797B1 (en) * 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
ES2118066T3 (es) 1989-10-05 1998-09-16 Optein Inc Sintesis y aislamiento, exentos de celulas, de nuevos genes y polipeptidos.
US6183967B1 (en) * 1995-06-07 2001-02-06 Nexstar Pharmaceuticals Nucleic acid ligand inhibitors to DNA polymerases
WO1998023733A2 (en) 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
EP1005481B1 (en) 1997-04-22 2009-10-14 Life Technologies Corporation Methods for the production of aslv reverse transcriptases composed of multiple subunits
ES2283936T3 (es) 1997-07-07 2007-11-01 Medical Research Council Procedimiento de clasificacion in vitro.
US6136582A (en) * 1998-01-20 2000-10-24 The Trustees Of Columbia University In The City Reverse transcriptase of moloney murine leukemia virus with RNA polymerase activity
JP4193079B2 (ja) 1998-11-10 2008-12-10 東洋紡績株式会社 変異型逆転写酵素
AU2611700A (en) * 1999-01-15 2000-08-01 Molecular Biology Resources Biologically active reverse transcriptases
WO2001068895A1 (en) 2000-03-15 2001-09-20 Invitrogen Corporation High fidelity reverse transcriptases and uses thereof
DK1268766T3 (da) 2000-03-31 2006-04-10 Cambridge Antibody Tech Forbedringer af ribosom-display
US7078208B2 (en) 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
CN1430670A (zh) * 2000-05-26 2003-07-16 茵维特罗根公司 热稳定逆转录酶及其用途
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
EP1436385A4 (en) 2001-09-14 2005-12-14 Invitrogen Corp DNA POLYMERASES AND MUTANTS CORRESPONDING
GB0127564D0 (en) * 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
GB2388031B (en) 2002-04-30 2005-07-27 Abbott Lab A lancet removal tool
US7842476B2 (en) * 2002-09-06 2010-11-30 Isogenica Limited In vitro peptide expression library
GB0221053D0 (en) 2002-09-11 2002-10-23 Medical Res Council Single-molecule in vitro evolution
WO2005049787A2 (en) * 2003-11-24 2005-06-02 Yeda Research And Development Co.Ltd. Compositions and methods for in vitro sorting of molecular and cellular libraries
WO2005071072A1 (en) 2004-01-13 2005-08-04 The Institute Of Biophysics Chinese Academy Of Sciences The recombinant murine leukemia virus reverse transcriptase, the gene encoding it and the method for expressing it
US7595179B2 (en) 2004-04-19 2009-09-29 Applied Biosystems, Llc Recombinant reverse transcriptases
WO2006051552A2 (en) 2004-11-15 2006-05-18 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed evolution and selection using in vitro compartmentalization
JPWO2007011045A1 (ja) * 2005-07-20 2009-02-05 株式会社クラレ パージング剤
EP2074211B1 (en) 2006-09-06 2013-03-13 Medical Research Council DNA polymerases incorporating dye-labelled nucleotide analogues
GB0806562D0 (en) 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070141592A1 (en) * 2000-05-26 2007-06-21 Smith Michael D Thermostable reverse transcriptases and uses thereof
WO2007022045A2 (en) * 2005-08-10 2007-02-22 Stratagene California Mutant reverse transcriptase and methods of use

Also Published As

Publication number Publication date
SG10201503327QA (en) 2015-06-29
IL208578A0 (en) 2010-12-30
JP6473795B2 (ja) 2019-02-20
JP6236563B2 (ja) 2017-11-22
EP2639300B1 (en) 2017-08-09
JP2014158473A (ja) 2014-09-04
US10358670B2 (en) 2019-07-23
US20170298403A1 (en) 2017-10-19
US20120156752A1 (en) 2012-06-21
JP2017169575A (ja) 2017-09-28
EP3375870B1 (en) 2020-08-05
US20130288925A1 (en) 2013-10-31
KR20160062213A (ko) 2016-06-01
US20180298414A1 (en) 2018-10-18
NZ588468A (en) 2012-10-26
US8580548B2 (en) 2013-11-12
EP2639300A3 (en) 2014-03-26
WO2009125006A3 (en) 2010-03-11
KR101653775B1 (ko) 2016-09-05
EP2281035A2 (en) 2011-02-09
EP2639300A2 (en) 2013-09-18
CA2721117A1 (en) 2009-10-15
US8835148B2 (en) 2014-09-16
KR101828902B1 (ko) 2018-02-13
KR101947101B1 (ko) 2019-02-12
KR20190016132A (ko) 2019-02-15
WO2009125006A2 (en) 2009-10-15
IL246396B (en) 2018-04-30
PL2281035T3 (pl) 2015-07-31
IL208578A (en) 2016-07-31
US10287614B2 (en) 2019-05-14
AU2009235368A1 (en) 2009-10-15
CN107058258A (zh) 2017-08-18
GB0806562D0 (en) 2008-05-14
KR102082768B1 (ko) 2020-02-28
US20110065606A1 (en) 2011-03-17
EP3375870A1 (en) 2018-09-19
ES2647272T3 (es) 2017-12-20
JP2016073298A (ja) 2016-05-12
JP5642662B2 (ja) 2014-12-17
KR20180016635A (ko) 2018-02-14
EP2281035B1 (en) 2015-01-21
EP3098308B1 (en) 2018-11-14
JP6140792B2 (ja) 2017-05-31
JP2011516072A (ja) 2011-05-26
KR20110065420A (ko) 2011-06-15
CN102057039A (zh) 2011-05-11
JP2018046841A (ja) 2018-03-29
EP3098308A1 (en) 2016-11-30
US9683251B2 (en) 2017-06-20

Similar Documents

Publication Publication Date Title
US10358670B2 (en) Production of nucleic acid
US20240287452A1 (en) Engraftable cell-based immunotherapy for long-term delivery of therapeutic proteins
US9528137B2 (en) Methods for cell-free protein synthesis
ES2914591T3 (es) Procedimientos novedosos para la producción de oligonucleótidos
CN112831484B (zh) T7-rna聚合酶突变体及其应用
CN112313334A (zh) 用以编辑血红蛋白相关突变的同源定向修复模板设计和递送
CA3060523A1 (en) A platform for t lymphocyte genome engineering and in vivo high-throughput screening thereof
CN113423823A (zh) 经基因组编辑的细胞的制造方法
JP2021531037A (ja) 非標準アミノ酸含有組成物とその使用
CN115175992B (zh) 用于制备生产高浓度的用于7-adca制备的脱乙氧基头孢菌素c的重组顶头孢霉菌株的方法以及通过该方法制备的菌株
CN107475272A (zh) 一种具热稳定性且耐高盐的琼脂水解酶
US20040161753A1 (en) Creation and identification of proteins having new dna binding specificities
Sydow Structural basis of transcription: RNA polymerase II fidelity mechanisms and RNA 3'fraying

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired