ATE325884T1 - Verfahren zur einführung von genen in zielzellen mit hilfe von retroviren - Google Patents

Verfahren zur einführung von genen in zielzellen mit hilfe von retroviren

Info

Publication number
ATE325884T1
ATE325884T1 AT96937515T AT96937515T ATE325884T1 AT E325884 T1 ATE325884 T1 AT E325884T1 AT 96937515 T AT96937515 T AT 96937515T AT 96937515 T AT96937515 T AT 96937515T AT E325884 T1 ATE325884 T1 AT E325884T1
Authority
AT
Austria
Prior art keywords
target cells
retrovirus
retroviruses
effective amount
functional material
Prior art date
Application number
AT96937515T
Other languages
English (en)
Inventor
Kiyozo Asada
Takashi Uemori
Takashi Ueno
Nobuto Koyama
Kimikazu Hashino
Ikunoshiu Kato
Original Assignee
Takara Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Bio Inc filed Critical Takara Bio Inc
Application granted granted Critical
Publication of ATE325884T1 publication Critical patent/ATE325884T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1088—Glutathione transferase (2.5.1.18)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13045—Special targeting system for viral vectors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT96937515T 1995-11-13 1996-11-07 Verfahren zur einführung von genen in zielzellen mit hilfe von retroviren ATE325884T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP29438295 1995-11-13
JP5184796 1996-03-08

Publications (1)

Publication Number Publication Date
ATE325884T1 true ATE325884T1 (de) 2006-06-15

Family

ID=26392431

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96937515T ATE325884T1 (de) 1995-11-13 1996-11-07 Verfahren zur einführung von genen in zielzellen mit hilfe von retroviren

Country Status (13)

Country Link
US (5) US6472204B1 (de)
EP (1) EP0870839B1 (de)
JP (1) JP3343357B2 (de)
KR (2) KR100395420B1 (de)
CN (1) CN100390290C (de)
AT (1) ATE325884T1 (de)
AU (1) AU709831B2 (de)
CA (1) CA2235629A1 (de)
DE (1) DE69636122T2 (de)
EA (3) EA003195B1 (de)
ES (1) ES2259182T3 (de)
MX (1) MX9803706A (de)
WO (1) WO1997018318A1 (de)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7083979B1 (en) * 1994-03-25 2006-08-01 Indiana University Foundation Methods for enhanced retroviral-mediated gene transfer
DE69633565T3 (de) 1995-06-15 2013-01-17 Crucell Holland B.V. Verpackungssysteme für humane, menschliche adenoviren, zur verwendung in die gentherapie
CN1326999C (zh) * 1995-09-29 2007-07-18 印地安纳大学研究及科技有限公司 使用含有病毒和细胞结合区域的分子增强病毒介导的dna转移的方法
TWI239352B (en) * 1997-07-23 2005-09-11 Takara Bio Inc Gene transfer method with the use of serum-free medium
DE69840671D1 (de) * 1997-11-14 2009-04-30 Cedars Sinai Medical Center Transfektion und transfer nicht humaner männlicher keimzellen zur generierung transgener nicht humaner säugetiere
ES2316675T3 (es) * 1998-07-01 2009-04-16 Takara Bio Inc. Metodos de transferencia genica con retrovirus.
WO2000026393A1 (en) * 1998-10-31 2000-05-11 University Of Medicine And Dentistry Of New Jersey A myeloid precursor cell useful for gene therapy and for modulation of immune responses
ATE376840T1 (de) * 1999-03-23 2007-11-15 Takara Bio Inc Gentherapeutika
US7345019B1 (en) 1999-04-13 2008-03-18 The Kenneth S. Warren Institute, Inc. Modulation of excitable tissue function by peripherally administered erythropoietin
US7410941B1 (en) 1999-04-13 2008-08-12 The Kenneth S. Warren Institute, Inc. Methods for treatment of neurodegenerative conditions by peripherally administered erythropoietin
US20050164386A1 (en) * 1999-04-15 2005-07-28 Uytdehaag Alphonsus G. Overexpression of enzymes involved in post-translational protein modifications in human cells
US8236561B2 (en) * 1999-04-15 2012-08-07 Crucell Holland B.V. Efficient production of IgA in recombinant mammalian cells
US7604960B2 (en) * 1999-04-15 2009-10-20 Crucell Holland B.V. Transient protein expression methods
US7297680B2 (en) 1999-04-15 2007-11-20 Crucell Holland B.V. Compositions of erythropoietin isoforms comprising Lewis-X structures and high sialic acid content
US6855544B1 (en) * 1999-04-15 2005-02-15 Crucell Holland B.V. Recombinant protein production in a human cell
US20050170463A1 (en) * 1999-04-15 2005-08-04 Abraham Bout Recombinant protein production in permanent amniocytic cells that comprise nucleic acid encoding adenovirus E1A and E1B proteins
GB9912965D0 (en) * 1999-06-03 1999-08-04 Oxford Biomedica Ltd In vivo selection method
AU5958000A (en) * 1999-07-08 2001-01-30 Connie Jean Eaves Method to improve viral infection
US7192759B1 (en) * 1999-11-26 2007-03-20 Crucell Holland B.V. Production of vaccines
US7521220B2 (en) * 1999-11-26 2009-04-21 Crucell Holland B.V. Production of vaccines
US7527961B2 (en) * 1999-11-26 2009-05-05 Crucell Holland B.V. Production of vaccines
AU2001285263A1 (en) 2000-08-25 2002-03-13 The General Hospital Corporation Selective precipitation of viruses
US6531121B2 (en) 2000-12-29 2003-03-11 The Kenneth S. Warren Institute, Inc. Protection and enhancement of erythropoietin-responsive cells, tissues and organs
PA8536201A1 (es) 2000-12-29 2002-08-29 Kenneth S Warren Inst Inc Protección y mejoramiento de células, tejidos y órganos que responden a la eritropoyetina
CN1313158C (zh) 2001-06-20 2007-05-02 大日本住友制药株式会社 促进核酸转移的方法
JP2005052001A (ja) * 2001-07-05 2005-03-03 Takara Bio Inc 遺伝子治療剤
DE60232083D1 (de) 2001-08-15 2009-06-04 Takara Bio Inc Expansionskulturverfahren für antigenspezifische zytotoxische t-lymphozyten
CA2468957C (en) * 2001-12-07 2011-07-12 Crucell Holland B.V. Methods of propagation of influenza virus in cell lines over expressing sialyl transferases
KR100895231B1 (ko) 2002-03-25 2009-05-04 다카라 바이오 가부시키가이샤 세포상해성 림프구의 제조방법
US20100143442A1 (en) 2003-02-05 2010-06-10 Queensland University Of Technology Growth factor complexes and modulation of cell migration and growth
US20040220085A1 (en) * 2003-05-02 2004-11-04 Jasbir Sandhu Compositions for nucleic acid delivery
US20040219099A1 (en) * 2003-05-02 2004-11-04 Jasbir Sandhu Method for the treatment of tumors
EA008670B1 (ru) 2003-05-09 2007-06-29 Круселл Холланд Б.В. Культуры e1-иммортализованных клеток и способы их культивирования с целью повышения выхода их продукции
WO2004104046A1 (en) * 2003-05-23 2004-12-02 Crucell Holland B.V. Production of recombinant igm in per.c6 cells
FR2857598A1 (fr) * 2003-07-18 2005-01-21 Inst Nat Sante Rech Med Composes anti-angiogeniques, composition pharmaceutique les comprenant, et leur utilisation
US8927273B2 (en) 2003-08-22 2015-01-06 Takara Bio Inc. Process for producing cytotoxic lymphocytes
GB0410130D0 (en) * 2004-05-06 2004-06-09 Molmed Spa Cell preparation
CN101031651B (zh) * 2004-09-29 2010-06-16 宝生物工程株式会社 用于产生反转录病毒载体的细胞
WO2006082958A1 (ja) 2005-02-07 2006-08-10 Takara Bio Inc. レトロウイルスの保存方法
JP4860612B2 (ja) 2005-06-15 2012-01-25 タカラバイオ株式会社 脂肪細胞あるいは前駆脂肪細胞への遺伝子導入方法
AU2006274438A1 (en) * 2005-07-29 2007-02-01 Australian Stem Cell Centre Limited Compositions and methods for growth of pluripotent cells
WO2007020873A1 (ja) 2005-08-16 2007-02-22 Takara Bio Inc. 免疫不全ウイルス感染症の治療または予防のための核酸
KR101279172B1 (ko) 2005-08-17 2013-06-27 다카라 바이오 가부시키가이샤 림프구의 제조 방법
JP4741906B2 (ja) * 2005-08-31 2011-08-10 タカラバイオ株式会社 リンパ球の製造方法
AU2006298188B2 (en) * 2005-09-30 2012-03-15 Takara Bio Inc. Method for production of T cell population
US8216837B2 (en) 2006-06-09 2012-07-10 Takara Bio Inc. Method of producing lymphocytes
WO2008133137A1 (ja) 2007-04-20 2008-11-06 Takara Bio Inc. 遺伝子治療のためのベクター
JP5485139B2 (ja) 2008-03-27 2014-05-07 タカラバイオ株式会社 遺伝子導入細胞の製造方法
CN102144027B (zh) 2008-07-07 2016-04-06 宝生物工程株式会社 生产多能干细胞的方法
WO2010080032A2 (en) * 2009-01-09 2010-07-15 Stichting Het Nederlands Kanker Instituut Bead-assisted viral transduction
WO2011024791A1 (ja) 2009-08-25 2011-03-03 タカラバイオ株式会社 レチノイン酸存在下でのｔ細胞集団の製造方法
EP2476752A4 (de) 2009-09-11 2013-04-03 Takara Bio Inc Verfahren zur herstellung natürlicher killerzellen
KR101930916B1 (ko) * 2009-11-30 2018-12-19 티슈 테라피스 리미티드 피브로넥틴：성장 인자 키메라
JP5778147B2 (ja) * 2010-06-30 2015-09-16 タカラバイオ株式会社 遺伝子導入方法
US9228206B2 (en) 2010-06-30 2016-01-05 Takara Bio Inc. Method for gene transfer
US9861681B2 (en) 2013-04-18 2018-01-09 The United States of America as represented by the Secretary of the Army, on behalf of the United States Army Medical Research Institute of Infectious Diseases Therapeutic compositions for neutralizing type I interferons, and methods of use
RU2021134624A (ru) 2015-10-22 2022-03-15 Джуно Терапьютикс Гмбх Способы, наборы, средства и устройства для трансдукции
WO2018021543A1 (ja) 2016-07-29 2018-02-01 タカラバイオ株式会社 幹細胞の製造に用いられるフィブロネクチンフラグメント
US11524988B2 (en) 2016-09-19 2022-12-13 H. Lee Moffitt Cancer Center And Research Institute, Inc. Artificial antigen presenting cells for genetic engineering of immune cells
CN108103045B (zh) * 2016-11-25 2022-10-04 丰益(上海)生物技术研发中心有限公司 脂肪酶及其应用
WO2018156735A1 (en) * 2017-02-22 2018-08-30 H. Lee Moffitt Cancer Center And Research Institute, Inc. Bispecific antibody for cancer immunotherapy
EP3397998A4 (de) 2017-02-22 2019-04-17 Lumus Ltd. Optische lichtleiteranordnung
EP3744832A4 (de) 2018-01-25 2021-11-24 Takara Bio Inc. Verfahren zur herstellung von lymphozyten
US10983264B2 (en) 2019-01-24 2021-04-20 Lumus Ltd. Optical systems including light-guide optical elements with two-dimensional expansion
WO2021138303A1 (en) * 2019-12-30 2021-07-08 Saint-Gobain Performance Plastics Corporation Containers and methods for cell transduction
CN113444167B (zh) * 2021-07-15 2022-09-30 陕西巨子生物技术有限公司 重组人胶原蛋白多肽及其应用
CN114634984A (zh) * 2022-02-02 2022-06-17 复旦大学 一种脑胶质瘤生物标记物mlkl基因及其应用
WO2023213969A1 (en) * 2022-05-05 2023-11-09 Juno Therapeutics Gmbh Viral-binding protein and related reagents, articles, and methods of use
CN114965839A (zh) * 2022-05-11 2022-08-30 朗肽生物制药股份有限公司 人碱性成纤维细胞生长因子的肽图分析方法
WO2024108221A1 (en) * 2022-11-18 2024-05-23 Saint-Gobain Performance Plastics Corporation Aptamers, containers and methods for cell transduction
CN117603343B (zh) * 2024-01-19 2024-04-26 四川大学 阻断bFGF的胶原来源天然短肽及应用
CN117645664A (zh) * 2024-01-29 2024-03-05 英特菲尔(成都)生物制品有限责任公司 重组人纤连蛋白标准品及其制备方法、鉴定方法与应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8516421D0 (en) 1985-06-28 1985-07-31 Biotechnology Interface Ltd Fibronectins
US5026839A (en) 1985-12-17 1991-06-25 Synergen, Inc DNA encoding a basic fibroblast growth factor
JP2770926B2 (ja) * 1985-12-17 1998-07-02 サイナーゲン インコーポレーテッド 毛管内皮細胞プロテアーゼ合成、ｄｎａ合成および遊走を促進することの出来るヒト胎盤血管形成誘導因子
US4994559A (en) 1985-12-17 1991-02-19 Synergen, Inc. Human basic fibroblast growth factor
US5399346A (en) 1989-06-14 1995-03-21 The United States Of America As Represented By The Department Of Health And Human Services Gene therapy
US5492890A (en) 1990-12-03 1996-02-20 The Scripps Research Institute Polypeptides for promoting cell attachment
DE4110409C2 (de) * 1991-03-29 1999-05-27 Boehringer Ingelheim Int Neue Protein-Polykation-Konjugate
JP2829405B2 (ja) * 1991-10-14 1998-11-25 寳酒造株式会社 機能性ポリペプチド
SE503225C2 (sv) * 1991-10-30 1996-04-22 Leif Lindholm Konsult Ab Virus-antikroppskomplex för införing av virus i mammalieceller
CH685803A5 (fr) * 1992-06-09 1995-10-13 Francis Verdan Bâton d'alun fixé dans un étui.
GB9223084D0 (en) * 1992-11-04 1992-12-16 Imp Cancer Res Tech Compounds to target cells
US5686278A (en) * 1994-03-25 1997-11-11 Indiana University Foundation Methods for enhanced retrovirus-mediated gene transfer
JPH1029952A (ja) 1996-07-16 1998-02-03 Takara Shuzo Co Ltd ヒト免疫不全ウイルス感染の制御用組成物および制御方法

Also Published As

Publication number Publication date
EA200001110A1 (ru) 2001-06-25
US20090209730A1 (en) 2009-08-20
CN1207774A (zh) 1999-02-10
EA003204B1 (ru) 2003-02-27
EP0870839A4 (de) 2000-01-19
CN100390290C (zh) 2008-05-28
AU709831B2 (en) 1999-09-09
US7446170B2 (en) 2008-11-04
US6426042B1 (en) 2002-07-30
US20060030046A1 (en) 2006-02-09
CA2235629A1 (en) 1997-05-22
MX9803706A (es) 1998-09-30
ES2259182T3 (es) 2006-09-16
EP0870839B1 (de) 2006-05-10
EA004928B1 (ru) 2004-10-28
KR19990067446A (ko) 1999-08-16
EA200200837A1 (ru) 2002-12-26
EA199800369A1 (ru) 1998-12-24
US7790849B2 (en) 2010-09-07
US6472204B1 (en) 2002-10-29
DE69636122T2 (de) 2006-12-07
DE69636122D1 (de) 2006-06-14
KR100353115B1 (ko) 2003-01-06
EA003195B1 (ru) 2003-02-27
WO1997018318A1 (en) 1997-05-22
KR100395420B1 (ko) 2003-08-21
AU7505896A (en) 1997-06-05
JP3343357B2 (ja) 2002-11-11
EP0870839A1 (de) 1998-10-14
US6949623B2 (en) 2005-09-27
US20030087437A1 (en) 2003-05-08

Similar Documents

Publication Publication Date Title
ATE325884T1 (de) Verfahren zur einführung von genen in zielzellen mit hilfe von retroviren
ATE345682T1 (de) In vitro verfahren zum einbringen von nukleinsäuren in eine zelle
AU2161501A (en) Immobilized single domain antigen-binding molecules
ATE260981T1 (de) Vektor und verfahren zur einbringung von nukleinsäure in sich nicht teilende zellen
AU2894289A (en) Domain-modified constant region antibodies
DE69024447D1 (de) Induzierer einer tumorlysierenden Zelle, Verfahren und Gerät zur Induzierung tumorlysierender Zellen
DE69835813D1 (de) Verfahren zur kultivierung von zellen und zur vermehrung von viren
DE69809561D1 (de) Zusammensetzung und verfahren zur regulierung von zellproliferation und zelltod
DE59107591D1 (de) Immobilisierung von organischen makromolekülen oder biopolymeren in einer polymermembran
ATE70916T1 (de) Mit ueberzuegen versehene formkoerper zur bindung von bioaffinen substanzen.
EP1083980A4 (de) Modulation molekularer wechselwirkungspositionen in rns und anderen biomolekülen
DK0868511T3 (da) Genekspression af utrophin
DE69514415D1 (de) Verfahren zur Herstellung von Dioxazinverbindungen,damit gefärbte Materialien und Dioxazinverbindungen mit plattenartiger Kristallform.
ATE389727T1 (de) Modulatoren der expression von morphogenen und deren identifizierungsverfahren
DE69704885D1 (de) Zusammensetzung und Verfahren zur Leimung von flächigen Strukturen
RU2000106030A (ru) Способ воздействия на связывание p53 с геном-мишенью
DE59813114D1 (de) BK-RiV-Präparate zur Behandlung von proliferativen Zellerkrangungen
DE59607930D1 (de) Verfahren zur Einbringung von sich verjüngenden Pfählen, zugehörige Pfähle, und daraus hergestellte Gründungen und Fundamentbildungen
ATE55608T1 (de) Mittel und verfahren zur funktionalisierung von nukleinsaeuren.
DE3771490D1 (de) Filiformkorrosionsresistente primerzusammensetzung und methode zur verhuetung von filiformkorrosion.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties