AR020075A1 - Metodo y construccion para preparar una planta transgenica. - Google Patents

Metodo y construccion para preparar una planta transgenica.

Info

Publication number
AR020075A1
AR020075A1 ARP990102315A ARP990102315A AR020075A1 AR 020075 A1 AR020075 A1 AR 020075A1 AR P990102315 A ARP990102315 A AR P990102315A AR P990102315 A ARP990102315 A AR P990102315A AR 020075 A1 AR020075 A1 AR 020075A1
Authority
AR
Argentina
Prior art keywords
plant
gene
coix
transgenic
transgenic plant
Prior art date
Application number
ARP990102315A
Other languages
English (en)
Inventor
Alan L Kriz
Michael Luethy
Dale A Voyles
Original Assignee
Dekalb Genetics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dekalb Genetics Corp filed Critical Dekalb Genetics Corp
Publication of AR020075A1 publication Critical patent/AR020075A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un método para preparar una planta transgénica monocotiledonea distinta de coix que exprese un gen seleccionado que comprende los parsos de: (a)proveer ungen seleccionado; (b)preparar una construccion que contenga dicho gen ligado operativamente aun promotor Coix; (c)transformar una célula receptora de unaplanta monocotiledonea distinta de Coix con dicha construccion; y (d)regenerar una planta transgénica monocotiledonea que exprese dicho gen de dicha célulareceptora. La planta transgénicamono cotiledonea es una planta seleccionada del grupo formado por arroz, trigo, cebada, centeno, sorgo, y maíz y el gen puedeser potencialmente cualquier gen que se quiera expresar en plantas transgénicas. Se provee también un método para criarplantas qu e comprende los pasos de:(a)obtener una planta progenie de cualquier generacion de una planta transgenica monocotiledonea preparada de acuerdo con los métodos descriptos anteriormente,donde la planta progenie comprende dicha construccion;y (b)cruzar la planta consigo misma o con una segunda planta. Con este metodo, se evita elsilenciamiento de genes mediante el uso de secuencias homologas de plantas monocotiledoneas del género Coix para la transformacion. En estas secuenciasdetransgenes se inc luyen promotores, potenciales, secuencias de codificacion y terminadores de Coix. Es conveniente contar con alternativas de transgenesderivados de maíz para su expresion en maíz por cuanto el silenciamiento de genes basado enhomologías puede limita r o eliminar de forma efectiva la expresiontransgénica.
ARP990102315A 1998-05-14 1999-05-14 Metodo y construccion para preparar una planta transgenica. AR020075A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/078,972 US6635806B1 (en) 1998-05-14 1998-05-14 Methods and compositions for expression of transgenes in plants

Publications (1)

Publication Number Publication Date
AR020075A1 true AR020075A1 (es) 2002-04-10

Family

ID=22147323

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP990102315A AR020075A1 (es) 1998-05-14 1999-05-14 Metodo y construccion para preparar una planta transgenica.
ARP090102514A AR072694A2 (es) 1998-05-14 2009-07-03 Planta transgenica fertil

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP090102514A AR072694A2 (es) 1998-05-14 2009-07-03 Planta transgenica fertil

Country Status (21)

Country Link
US (5) US6635806B1 (es)
EP (1) EP1076706B1 (es)
JP (2) JP2002533057A (es)
KR (1) KR20010052350A (es)
CN (1) CN1310760A (es)
AR (2) AR020075A1 (es)
AT (1) ATE385518T1 (es)
AU (1) AU3995799A (es)
BR (1) BR9910455A (es)
CA (1) CA2328129C (es)
DE (1) DE69938101T2 (es)
DK (1) DK1076706T3 (es)
ES (1) ES2301239T3 (es)
ID (1) ID27936A (es)
IL (1) IL139568A0 (es)
IN (2) IN2000DE00321A (es)
MX (1) MXPA00011199A (es)
PT (1) PT1076706E (es)
TR (1) TR200100104T2 (es)
WO (1) WO1999058659A2 (es)
ZA (1) ZA200006576B (es)

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US7803928B2 (en) 2010-09-28
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US7803927B2 (en) 2010-09-28
US20080271212A1 (en) 2008-10-30
JP2002533057A (ja) 2002-10-08
EP1076706B1 (en) 2008-02-06
US20050250938A1 (en) 2005-11-10
CA2328129C (en) 2012-12-11
BR9910455A (pt) 2001-11-27
AR072694A2 (es) 2010-09-15
IN2000DE00321A (es) 2008-06-20
DE69938101D1 (de) 2008-03-20
PT1076706E (pt) 2008-05-09
ATE385518T1 (de) 2008-02-15
WO1999058659A3 (en) 2000-01-20
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US20090013423A1 (en) 2009-01-08
WO1999058659A2 (en) 1999-11-18
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US6635806B1 (en) 2003-10-21
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