Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
  • C07D405/14—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing three or more hetero rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
  • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
  • A61K31/506—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim not condensed and containing further heterocyclic rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
  • C07D403/14—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing three or more hetero rings
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
  • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
  • C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
  • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
  • A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
  • A61K31/443—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a five-membered ring with oxygen as a ring hetero atom
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
  • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
  • A61K31/513—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim having oxo groups directly attached to the heterocyclic ring, e.g. cytosine

Definitions

• Eukaryotic chromatin is composed of macromolecular complexes called nucleosomes.
• a nucleosome has 147 base pairs of DNA wrapped around a protein octamer having two subunits of each of histone protein H2A, H2B, H3, and H4.
• Histone proteins are subject to post-translational modifications which in turn affect chromatin structure and gene expression.
• One type of post-translational modification found on histones is methylation of lysine and arginine residues.
• Histone methylation plays a critical role in the regulation of gene expression in eukaryotes. Methylation affects chromatin structure and has been linked to both activation and repression of transcription (Zhang and Reinberg, Genes Dev. 15:2343- 2360, 2001).
• Enzymes that catalyze attachment and removal of methyl groups from histones are implicated in gene silencing, embryonic development, cell proliferation, and other processes.
• EZH2 is an example of a human SET-domain containing methylase. EZH2 associates with EED (Embryonic Ectoderm Development) and SUZ12 (suppressor of zeste 12 homolog) to form a complex known as PRC2 (Polycomb Group Repressive Complex 2) having the ability to tri-methylate histone H3 at lysine 27 (Cao and Zhang, Mol. Cell 15:57-67, 2004). PRC2 complexes can also include RBAP46 and RBAP48 subunits. Another example is the related methylase EZH1.
• EZH2 oncogenic activities of EZH2 have been shown by a number of studies in various different cancer types. -15-20% GCB-DLBCLs harbor a gain-of-function mutation in EZH2 (Y641 residue) and these cells are hypersensitive to EZH2 inhibition both in vitro and in vivo (McCabe et al, 2012; Bradley et al, 2014). In cell line experiments, over-expression of EZH2 induces cell invasion, growth in soft agar, and motility, while knockdown of EZH2 inhibits cell proliferation and cell invasion (Kleer et al., 2003, Proc. Nat. Acad. Sci.
• EZH2 is over-expressed in neuroendocrine tumors and inhibition of EZH2 in mouse tumors restore androgen dependence (Ku et al, Science, 355, 2017). EZH2 overexpression is associated with aggressiveness of certain cancers such as breast cancer (Kleer et al., Proc. Nat. Acad. Sci. ETSA 100:11606-11611, 2003).
• TMPRSS2-ERG induces repressive epigenetic programs via direct activation of EZH2 (Yu et al.,“An Integrated Network of Androgen Receptor, Polycomb, and TMPRSS2-ERG Gene Fusions in Prostate Cancer Progression,” Cancer Cell. 2010 May 18;17(5):443-454).
• methyl modifying enzymes in particular EZH2 and mutant forms thereof, are attractive targets for modulation.
• R 1 , R 2 , R 3 , R 4 , R 5 , and X are defined herein.
• certain compounds described herein have increased residence time (e.g., > 100 hours) and enhanced permeability.
• An increase in residence time (i.e., slower off rate) and/or permeability has been found to correlate with improved cellular potency.
• the disclosed compounds, pharmaceutically acceptable salts, and pharmaceutically acceptable compositions are useful for treating a variety of conditions associated with methyl modifying enzymes. These conditions include e.g., one or more cancers.
• R 1 is halo, -S(Ci-C 4 )alkyl, -S(C 3 -C 7 )cycloalkyl, or -S[halo(Ci-C 4 )alkyl];
• X is CH or N
• R is hydrogen, halo, (Ci-C 4 )alkyl, or halo(Ci-C 4 )alkyl;
• R 3 is halo, (Ci-C 4 )alkyl, or halo(Ci-C 4 )alkyl;
• R 4 is (C 3 -C 7 )cycloalkyl or 4-7 membered heterocyclyl, each of which are optionally substituted with 1 to 3 groups selected from halo, (Ci-C 4 )alkyl, halo(Ci-C 4 )alkyl, (Ci- C 4 )alkoxy, halo(Ci-C 4 )alkoxy, and -NR a R b ;
• R a is hydrogen, (Ci-C 4 )alkyl, or halo(Ci-C 4 )alkyl
• R b is (Ci-C 4 )alkyl, halo(Ci-C 4 )alkyl, or 4-7 membered heterocyclyl, wherein said heterocyclyl is optionally substituted with 1 to 3 groups selected from halo, (Ci-C 4 )alkyl, halo(Ci-C 4 )alkyl, (Ci-C 4 )alkoxy, and halo(Ci-C 4 )alkoxy; or
• R a and R b together with the nitrogen atom to which they are attached form a 4-7 membered heterocyclyl optionally substituted with 1 to 3 groups selected from halo, (Ci- C 4 )alkyl, halo(Ci-C 4 )alkyl, and -OR c ;
• R c is (Ci-C 4 )alkyl, halo(Ci-C 4 )alkyl, or (C 3 -C 7 )cycloalkyl;
• R 5 is halo, (Ci-C 4 )alkyl, or halo(Ci-C 4 )alkyl.
• a hyphen designates the point of attachment of that group to the variable to which it is defined.
• -S(C 3 -C 7 )cycloalkyl and -S[halo(Ci-C 4 )alkyl] mean that the point of attachment for this group occurs on the sulfur atom.
• halo and“halogen” as used herein refer to an atom selected from fluorine (fluoro, -F), chlorine (chloro, -Cl), bromine (bromo, -Br), and iodine (iodo, -I).
• alkyl refers to a monovalent saturated, straight- or branched-chain hydrocarbon radical, having unless otherwise specified, 1-10 carbon atoms.
• alkyl radicals include, but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, iso-butyl, sec-butyl, sec -pentyl, iso-pentyl, tert-butyl, n-pentyl, neopentyl, n-hexyl, sec-hexyl, and the like.
• haloalkyl includes mono, poly, and perhaloalkyl groups where the halogens are independently selected from fluorine, chlorine, bromine, and iodine.
• Alkoxy is an alkyl group which is attached to another moiety via an oxygen linker (-O(alkyl)).
• oxygen linker -O(alkyl)
• Non-limiting examples include methoxy, ethoxy, propoxy, and butoxy.
• Haloalkoxy is a haloalkyl group which is attached to another moiety via an oxygen atom such as, e.g., but are not limited to -OCHCF 2 or -OCF 3 .
• cycloalkyl refers to a 3- to l2-membered (e.g., 3- to 7-membered) monocyclic, bicyclic (e.g., a bridged or spiro bicyclic ring), or polycyclic (e.g., tricyclic), hydrocarbon ring system that is completely saturated.
• Monocyclic cycloalkyl groups include, without limitation, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl.
• Bridged bicyclic cycloalkyl groups include, without limitation,
• Spiro bicyclic cycloalkyl groups include, e.g., spiro [3.6] decane, spiro[4.5]decane, and the like.
• Fused cycloalkyl rings include, e.g., decahydronaphthalene, octahydropentalene, and the like.
• substituents on a cycloalkyl may be present on any substitutable position and, include, e.g., the position at which the cycloalkyl group is attached.
• heterocyclyl means a 3-12 membered (e.g., a 4-, 5-, 6- and 7- membered) saturated or partially unsaturated heterocyclic ring containing 1 to 4 heteroatoms independently selected from N, O, and S. It can be mononcyclic, bicyclic (e.g., a bridged, fused, or spiro bicyclic ring), or tricyclic.
• the terms“heterocycle”,“heterocyclyl”, “heterocyclyl ring”,“heterocyclic group”,“heterocyclic moiety”, and“heterocyclic radical”, are used interchangeably herein.
• a heterocyclyl ring can be attached to its pendant group at any heteroatom or carbon atom that results in a stable structure.
• saturated or partially unsaturated heterocyclic radicals include, without limitation, tetrahydrofuranyl, tetrahydrothienyl, terahydropyranyl, pyrrolidinyl, pyridinonyl, pyrrolidonyl, piperidinyl, oxazolidinyl, piperazinyl, dioxanyl, dioxolanyl, morpholinyl, dihydrofuranyl, dihydropyranyl, dihydropyridinyl, tetrahydropyridinyl, dihydropyrimidinyl, oxetanyl, azetidinyl and tetrahydropyrimidinyl.
• heterocyclyl group may be mono- or bicyclic.
• heterocyclyl also includes, e.g., unsaturated heterocyclic radicals fused to another unsaturated heterocyclic radical or aryl or heteroaryl ring, such as for example,
• substituents on a heterocyclyl may be present on any substitutable position and, include, e.g., the position at which the heterocyclyl group is attached.
• spiro refers to two rings that shares one ring atom (e.g., carbon).
• fused refers to two rings that share two adjacent ring atoms with one another.
• bridged refers to two rings that share three or more ring atoms with one another.
• geometric isomer refers to isomers that differ in the orientation of substituent atoms in relationship to a cycloalkyl ring, i.e., cis or trans isomers.
• cis or trans isomers that differ in the orientation of substituent atoms in relationship to a cycloalkyl ring.
• the stereochemistry of a disclosed compound is named or depicted by structure
• the named or depicted stereoisomer is at least 60%, 70%, 80%, 90%, 99% or 99.9% by weight pure relative to all of the other stereoisomers.
• the depicted or named enantiomer is at least 60%, 70%, 80%, 90%, 99% or 99.9% by weight optically pure. Percent optical purity by weight is the ratio of the weight of the enantiomer over the weight of the enantiomer plus the weight of its optical isomer.
• Enantiomers may be resolved by methods known to those skilled in the art, for example by formation of diastereoisomeric salts which may be separated, for example, by crystallization; formation of diastereoisomeric derivatives or complexes which may be separated, for example, by crystallization, supercritical fluid or liquid chromatography; selective reaction of one enantiomer with an enantiomer- specific reagent, for example enzymatic esterification; or supercritical fluid or liquid chromatography in a chiral environment, for example on a chiral support for example silica with a bound chiral ligand or in the presence of a chiral solvent.
• Specific enantiomers may also be synthesized by asymmetric synthesis using optically active reagents, substrates, catalysts or solvents, or by converting one enantiomer into the other by asymmetric transformation.
• the named or depicted configuration is enriched relative to the remaining configurations, for example, by a molar excess of at least 60%, 70%, 80%, 90%, 99% or 99.9%.
• the substructure :
• stereochemically enriched as R e.g., by a molar excess of at least 60%, 70%, 80%, 90%, 99% or 99.9%
• geometry about the cyclohexyl may be cis or trans, or a mixture thereof.
• a disclosed compound is depicted by structure without indicating the stereochemistry, but is identified as being stereochemically enriched (e.g., “single enantiomer; single geometric isomer” or“single enantiomer” in instances where geometric isomers are not possible).
• stereochemically enriched e.g., “single enantiomer; single geometric isomer” or“single enantiomer” in instances where geometric isomers are not possible.
• single enantiomer single geometric isomer
• the configuration about the chiral dioxolanyl carbon is stereochemically enriched as R (e.g., by a molar excess of at least 60%, 70%, 80%, 90%, 99% or 99.9%) or is stereochemically enriched as S (e.g., by a molar excess of at least 60%, 70%, 80%, 90%, 99% or 99.9%) and that the geometric configuration about the cyclohexyl ring is enriched as cis (e.g., by a molar excess of at least 60%, 70%, 80%, 90%, 99% or 99.9%) or is enriched as trans (e.g., by a molar excess of at least 60%, 70%, 80%, 90%, 99% or 99.9%).
• the term“patient,” as used herein, means an animal, such as a mammal, and such as a human.
• the terms“subject” and“patient” may be used interchangeably.
• compositions described herein include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, cellulose-based substances, polyethylene glycol, sodium carboxymethylcellulose, polyacrylates, waxes, polyethylene-polyoxypropylene-block polymers,
• treatment refers to reversing, alleviating, or inhibiting the progress of a disease or disorder, or one or more symptoms thereof, as described herein. Treatment may also be continued after symptoms have resolved, for example to delay recurrence.
• the term“effective amount” or“therapeutically effective amount” refers to an amount of a compound described herein that will elicit a biological or medical response of a subject e.g., a dosage of between 0.01 - 100 mg/kg body weight/day.
• the terms“subject” and “patient” may be used interchangeably, and means a mammal in need of treatment, e.g., companion animals (e.g., dogs, cats, and the like), farm animals (e.g., cows, pigs, horses, sheep, goats and the like) and laboratory animals (e.g., rats, mice, guinea pigs and the like).
• the subject is a human in need of treatment.
• the term“inhibit,”“inhibition” or“inhibiting” includes a decrease in the baseline activity of a biological activity or process.
• the compounds described herein may be present in the form of pharmaceutically acceptable salts.
• the salts of the compounds described herein refer to non-toxic“pharmaceutically acceptable salts.”
• Pharmaceutically acceptable salt forms include pharmaceutically acceptable acidic/anionic or basic/cationic salts.
• Suitable pharmaceutically acceptable acid addition salts of the compounds described herein include e.g., salts of inorganic acids (such as hydrochloric acid, hydrobromic, phosphoric, nitric, and sulfuric acids) and of organic acids (such as, acetic acid, benzenesulfonic, benzoic, methanesulfonic, and p-toluenesulfonic acids).
• R 1 is halo or -S(Ci-C 4 )alkyl
• R 2 is halo
• R 3 is (Ci-C 4 )alkyl
• R 4 is (C 3 -C 7 )cycloalkyl or (C 4 -C 7 )heterocyclyl, each of which are optionally substituted with 1 to 2 groups selected from halo(Ci-C 4 )alkyl and -NR a R b ;
• R a is hydrogen or (Ci-C 4 )alkyl
• R b is (Ci-C 4 )alkyl or (C 4 -C 7 )heterocyclyl, wherein said heterocyclyl is optionally substituted with halo(Ci-C 4 )alkyl; or
• R a and R b together with the nitrogen atom to which they are attached form a 4-7 membered nitrogen containing heterocyclyl optionally substituted with 1 to 3 groups selected from halo and -OR c ;
• R c is (Ci-C 4 )alkyl, halo(Ci-C 4 )alkyl, or (C 3 -C 7 )cycloalkyl;
• R 5 is halo or (Ci-C 4 )alkyl.
• the compound of Formula I is of Formula II:
• R 1 in the compound of Formula I or Formula II is chloro, wherein the remaining variables are as described above for Formula I or the second embodiment.
• R 1 in the compound of Formula I or Formula II is -SCH 3 , wherein the remaining variables are as described above for Formula I or the second embodiment.
• R 4 in the compound of Formula I or Formula II is cyclohexyl or piperidinyl, each of which are optionally substituted with 1 to 2 groups selected from halo(Ci-C 4 )alkyl and -NR a R b , wherein the remaining variables are as described above for Formula I or the second, fourth, or fifth embodiment.
• the compound of Formula I or Formula II is of the Formula III:
• R 6 is halo(Ci-C 4 )alkyl, and wherein the remaining variables are as described above for Formula I or the second, fourth, or fifth embodiment.
• the compound of Formula I, Formula II, or Formula III is of the Formula IV :
• the compound of Formula I or Formula II is of the Formula V :
• the compound of Formula I, Formula II, or Formula V is of the Formula VI or VII:
• Formula V, Formula VI, and Formula VII is (Ci-C 4 )alkyl or oxatanyl, wherein said oxetanyl is optionally substituted with halo(Ci-C 4 )alkyl; or R a and R b together with the nitrogen atom to which they are attached form an azetidinyl optionally substituted with halo or -OR c , wherein the remaining variables and features are as described above for Formula I or the second, fourth, or fifth embodiment.
• R a in the compounds of Formula I, Formula II, Formula V, Formula VI, and Formula VII is hydrogen or methyl; and R b is methyl or oxatanyl, wherein said oxetanyl is optionally substituted with -CH 2 F or -CF 3 , wherein the remaining variables and features are as described above for Formula I or the second, fourth, fifth, or eleventh embodiment.
• R a and R b in the compounds of Formula I, Formula II, Formula V, Formula VI, and Formula VII together with the nitrogen atom to which they are attached form an azetidinyl optionally substituted with 1 to 2 fluoro or -OR c ; and R c is -CH 3 , -CHF 2 , or cyclopropyl, wherein the remaining variables and features are as described above for Formula I or the second, fourth, fifth, or eleventh embodiment.
• the configuration of the l,3-dioxolanyl and the NR a R b group in any one of Formula I, II, V, VI, and VII are oriented trans about the cyclohexyl, wherein the remaining features are as described above for Formula I or the second, fourth, fifth, sixth, eleventh, twelfth, or thirteenth embodiments.
• the configuration of the l,3-dioxolanyl and the NR a R b group of Formula I, II, V, VI, and VII, are oriented cis about the cyclohexyl, wherein the remaining features are as described above for Formula I or the second, fourth, fifth, sixth, eleventh, twelfth, or thirteenth embodiment.
• the stereochemical configuration of the chiral center of the l,3-dioxolanyl in any one of Formula I, II, III, IV, V, VI, and VII is R, wherein the remaining features are as described above for Formula I or the second, fourth, fifth, sixth, eleventh, twelfth, thirteenth, or fourteenth embodiment.
• V, VI, and VII is S, wherein the remaining features are as described above for Formula I or the second, fourth, fifth, sixth, eleventh, twelfth, thirteenth, or fourteenth embodiment.
• the present disclosure provides a composition comprising a compound described herein or a pharmaceutically acceptable derivative thereof and a pharmaceutically acceptable carrier.
• the amount of compound in a provided composition is such that is effective to measurably modulate a histone methyl modifying enzyme, or a mutant thereof, in a biological sample or in a patient.
• compositions described herein are formulated for administration to a patient in need of such composition.
• Compositions described herein may be administered orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally or via an implanted reservoir.
• parenteral as used herein includes subcutaneous, intravenous, intramuscular, intra- articular, intra- synovial, intrastemal, intrathecal, intrahepatic, intralesional and intracranial injection or infusion techniques.
• the compositions are administered orally, intraperitoneally or intravenously.
• Sterile injectable forms of the compositions described herein may be aqueous or oleaginous suspension. These suspensions may be formulated according to techniques known in the art using suitable dispersing or wetting agents and suspending agents.
• compositions are administered orally.
• a specific dosage and treatment regimen for any particular patient will depend upon a variety of factors, including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, rate of excretion, drug combination, and the judgment of the treating physician and the severity of the particular disease being treated.
• the amount of a compound described herein in the composition will also depend upon the particular compound in the composition.
• Compounds and compositions described herein are generally useful for the modulating of activity of one or more enzymes involved in epigenetic regulation and in particular EZH1 and EZH2 and, even more specifically EZH2 and mutant forms thereof.
• compounds described herein down-regulate or suppress the activity of EZH2.
• compounds described herein are antagonists of EZH2 activity.
• compounds described herein down-regulate or suppress the activity of EZH1.
• compounds described herein are antagonists of EZH1 activity.
• compounds and compositions described herein are useful in treating diseases and/or disorders associated with overexpression of EZH1 or EZH2 and/or expression of a mutant form of EZH2, such as those mutant forms that alter EZH2 substrate activity.
• EZH2 deletions, missense and frameshift mutations suggest that EZH2 functions as a tumor suppressor in blood disorders such as myelodysplastic syndromes (MDS) and myeloid malignancies (Ernst et al., Nat Genet. 2010 Aug; 42(8):722-6; Nikoloski et ah, Nat Genet. 2010 Aug; 42(8):665-7).
• compounds and compositions described herein are useful in treating diseases and/or disorders associated with the presence of EZH2 having a Y641N, Y641C, Y641F, Y641H, Y641S, A677G, or A687 mutation.
• the EZH2 has a Y641N mutation.
• the present disclosure provides a method of treating a subject suffering from a disease and/or disorder associated with overexpression of EZH1 or EZH2 and/or expression of a mutant form of EZH2 comprising the step of administering a compound described herein, or a pharmaceutically acceptable salt or composition thereof.
• the above method additionally comprises the preliminary step of determining if the subject is overexpressing EZH2 or expressing a mutant form of EZH2.
• the disease or disorder associated with the presence of a mutant form of EZH2 is a human B cell lymphoma.
• the disease and/or disorder associated with the presence of Y641N EZH2 is follicular lymphoma or diffuse large-B-cell lymphoma.
• compounds or compositions described herein are useful in treating blood disorders, such as myelodysplastic syndromes, leukemia, anemia and cytopenia. Sneeringer et al.,“Coordinated activities of wild-type plus mutant EZH2 drive tumor-associated hypertrimethylation of lysine 27 on histone H3
• compounds and compositions described herein are useful in treating diseases and/or disorders associated with cellular proliferation. In some embodiments, compounds and compositions described herein are useful in treating diseases and/or disorders associated with misregulation of cell cycle or DNA repair. In some embodiments, compounds and compositions described herein are useful in treating cancer.
• cancers that may be treated by the compounds, compositions and methods described herein include, but are not limited to Cardiac: sarcoma (angiosarcoma, fibrosarcoma, rhabdomyosarcoma, liposarcoma), myxoma, rhabdomyoma, fibroma, lipoma and teratoma; Lung: bronchogenic carcinoma (squamous cell, undifferentiated small cell, undifferentiated large cell, adenocarcinoma), alveolar (bronchiolar) carcinoma, bronchial adenoma, sarcoma, lymphoma, chondromatous hamartoma, mesothelioma; Gastrointestinal: esophagus (squamous cell carcinoma, adenocarcinoma, leiomyosarcoma, lymphoma), stomach (carcinoma, lymphoma, leiomyosarcom
• kidney adenocarcinoma, Wilm's tumor (nephroblastoma), lymphoma, leukemia), bladder and urethra (squamous cell carcinoma, transitional cell carcinoma, adenocarcinoma), prostate (adenocarcinoma, sarcoma), testis (seminoma, teratoma, embryonal carcinoma, teratocarcinoma,
• medulloblastoma glioma, ependymoma, germinoma (pinealoma), glioblastoma multiform, oligodendroglioma, schwannoma, retinoblastoma, congenital tumors), spinal cord
• uterus endometrial carcinoma
• cervix cervical carcinoma, pre-tumor cervical dysplasia
• ovaries ovarian carcinoma (serous cystadenocarcinoma, mucinous cystadenocarcinoma, clear cell carcinoma, unclassified carcinoma), granulosa-thecal cell tumors, Sertoli-Leydig cell tumors, dysgerminoma, malignant teratoma), vulva (squamous cell carcinoma, intraepithelial carcinoma, adenocarcinoma, fibrosarcoma, melanoma), vagina (clear cell carcinoma, squamous cell carcinoma, botryoid sarcoma (embryonal rhabdomyosarcoma), fallopian tubes (carcinoma); Hematologic: blood (myeloid leukemia (acute and chronic), acute lymphoblast
• the cancer treated by the compounds, compositions, and methods described herein is selected from adrenal cancer, acinic cell carcinoma, acoustic neuroma, acral lentiginous melanoma, acrospiroma, acute eosinophilic leukemia, acute erythroid leukemia, acute lymphoblastic leukemia, acute megakaryoblastic leukemia, acute monocytic leukemia, acute promyelocytic leukemia, adenocarcinoma, adenoid cystic carcinoma, adenoma, adenomatoid odontogenic tumor, adenosquamous carcinoma, adipose tissue neoplasm, adrenocortical carcinoma, adult T-cell leukemia/lymphoma, aggressive NKcell leukemia, AIDS-related lymphoma, alveolar rhabdomyosarcoma, alveolar soft part sarcoma, amelobl
• angioimmunoblastic T-cell lymphoma angiomyolipoma, angiosarcoma, astrocytoma, atypical teratoid rhabdoid tumor, B-cell chronic lymphocytic leukemia, B-cell
• prolymphocytic leukemia B-cell lymphoma, basal cell carcinoma, biliary tract cancer, bladder cancer, blastoma, bone cancer, Brenner tumor, Brown tumor, Burkitt's lymphoma, breast cancer, brain cancer, carcinoma, carcinoma in situ, carcinosarcoma, cartilage tumor, cementoma, myeloid sarcoma, chondroma, chordoma, choriocarcinoma, choroid plexus papilloma, clear-cell sarcoma of the kidney, craniopharyngioma, cutaneous T-cell lymphoma, cervical cancer, colorectal cancer, Degos disease, desmoplastic small round cell tumor, diffuse large B-cell lymphoma, dysembryoplastic neuroepithelial tumor, dysgerminoma, embryonal carcinoma, endocrine gland neoplasm, endodermal sinus tumor, enteropathy associated T-cell lymphoma, esophageal cancer, f
• the cancer treated by the compounds, compositions, and methods described herein is selected from adenocarcinoma, adult T-cell leukemia/lymphoma, bladder cancer, blastoma, bone cancer, breast cancer, brain cancer, carcinoma, myeloid sarcoma, cervical cancer, colorectal cancer, esophageal cancer, gastrointestinal cancer, glioblastoma multiforme, glioma, gallbladder cancer, gastric cancer, head and neck cancer, Hodgkin's lymphoma, non-Hodgkin's lymphoma, intestinal cancer, kidney cancer, laryngeal cancer, leukemia, lung cancer, lymphoma, liver cancer, small cell lung cancer, non-small cell lung cancer, mesothelioma, multiple myeloma, acute myeloid leukemia (AML), diffuse large B- cell lymphoma (DLBCL), ocular cancer, optic nerve tumor, oral cancer, ovarian cancer, pituit
• the cancer treated by the compounds, compositions, and methods described herein are selected from breast cancer, prostate cancer, colon cancer, renal cell carcinoma, glioblastoma multiforme cancer, bladder cancer, melanoma, bronchial cancer, lymphoma and liver cancer.
• composition comprising a disclosed compound or pharmaceutically acceptable salt thereof, for use in treating a condition described herein.
• Step 1 Synthesis of sodium 3-oxobut-l-ene-l,l-t>is(thiolate)
• Step 2 Synthesis of 4,4-bis(methylthio)but-3-en-2-one
• Step 4 Synthesis of 3-(aminomethyl)-6-methyl-4-(methylthio)pyridin-2(lH)- one
• Step 5 Synthesis of tert-butyl ((6-methyl-4-(methylthio)-2-oxo-l,2- dihydropyridin-3-yl)methyl)carbamate
• Step 1 Synthesis of methyl 2-cyano-3,3-bis(methylthio)acrylate
• Step 3 Synthesis of tert- butyl ((2-methyl-4-(methylthio)-6-oxo-l,6- dihydropyrimidin-5-yl)methyl)carbamate
• Step 4 Synthesis of (2-(benzyloxy)-6-chloro-4-(methylthio)pyridin-3- yl)methanamine
• Step 6 Synthesis of tert-butyl ((6-chloro-4-(methylthio)-2-oxo-l,2- dihydropyridin-3-yl)methyl)carbamate
• Step 7 Synthesis of 3-(aminomethyl)-6-chloro-4-(methylthio)pyridin-2(lH)- one (hydrochloride salt)
• Step 1 Synthesis of methyl 5-chloro-3,4-dihydroxy-2-methylbenzoate
• Step 2 Synthesis of methyl 7-chloro-2,4-dimethyl-2-(4-oxocyclohexyl)-2H- l,3-benzodioxole-5-carboxylate
• Step 3 Separation of methyl (R)-7-chloro-2,4-dimethyl-2-(4- oxocyclohexyl)benzo[d][l,3]dioxole-5-carboxylate and methyl (S)-7-chloro-2,4-dimethyl- 2-(4-oxocyclohexyl)benzo[d][l,3]dioxole-5-carboxylate
• Step 3 Synthesis of 4-hyriroxy-2-Tnethoxy-6-methylnicotinonitriIe
• Step 5 Synthesis of (4-chloro-2-methoxy-6-Hiethyipyridm-3-yl)methanamine
• Step 6 tert- butyl ((4-chloro-2-methexy-6-inethylpyridin-3-
• Step 7 3-(aTninornethyl)-4-chloro-6-rnethylpyridin-2(1ff)-one
• Step 1 Synthesis of methyl 7-chloro-2-(4-(3,3-difluoroazetidin-l- yI)cyclohexyl)-2,4-dimethylbenzo[d][l,3]dioxoIe-5-carbexylate
• a solution of methyl 7-chloro-2,4-dimethyl-2-(4- oxocyclohexyl)benzo[d][l,3]dioxole-5-carboxylate (Intermediate 4 - racemic mixture) 550 mg, 1.62 mmol), 3,3-difluoroazetidine hydrochloride salt (839 mg, 6.48 mmol) and triethylamine (895 pL, 6.64 mmol) in methanol (5 mL) and tetrahydrofuran (5 mL) was stirred overnight at room temperature.
• Step 2 Separation of methyl (2R)-7-chloro-2-(4-(3,3-difluoroazetidin-l- yl)cyclohexyl)-2,4-dimethylbenzo[d][l,3]dioxole-5-carboxylate and methyl (2S)-7- chloro-2-(4-(3,3-difluoroazetidin-l-yl)cyclohexyl)-2,4-dimethylbenzo[d][l,3]dioxole-5- carboxylate
• Step 1 Synthesis of tert-butyl 4-(7-chloro-5-(methoxycarbonyl)-2,4- dimethylbenzo[d][l,3]dioxol-2-yl)piperidine-l-carboxylat
• Step 2 Synthesis of methyl 7-chloro-2,4-dimethyl-2-(piperidin-4- yl)benzo[d][l,3]dioxole-5-carboxylate (trifluoroacetic acid salt)
• Step 3 Synthesis of methyl 7-chloro-2,4-dimethyl-2-(l-(2,2,2- trifluoroethyl)piperidin-4-yl)benzo[d][l,3]dioxole-5-carboxylate
• Step 4 Synthesis of 7-chloro-2,4-dimethyl-2-(l-(2,2,2- trifluoroethyl)piperidin-4-yl)benzo[d][l,3]dioxole-5-carboxylic acid
• Step 5 Synthesis of perfluorophenyl 7-chloro-2,4-dimethyl-2-(l-(2,2,2- trifluoroethyl)piperidin-4-yl)benzo[d][l,3]dioxole-5-carboxylate
• Step 1 Synthesis of methyl 7-chloro-2-(4-(3-methoxyazetidin-l- yl)cyclohexyl)-2,4-dimethylbenzo[d][l,3]dioxole-5-carboxylate
• Lithium borohydride (500 mg, 22.96 mmol) was added and the reaction stirred at -70 °C for 30 min [or until complete consumption of the starting material was observed by TLC, ethyl acetate/methanol 5:1].
• two batches of the reaction were combined and quenched with a saturated aqueous solution of ammonium chloride (120 mL) at 0 °C and the desired product was extracted with dichloromethane (200 mL x 3). The combined organic layers were dried over sodium sulfate, filtered and concentrated to dryness under reduced pressure. The residue was purified by flash
• Step 2 Synthesis of 7-chloro-2-(4-(3-methoxyazetidin-l-yl)cyclohexyl)-2,4- dimethylbenzo[d] [ 1 ,3 ]dioxole-5-carboxylic acid
• Example #1 7-chloro-2-(4-(3-(difluoromethoxy)azetidin-l-yl)cyclohexyl)-2, 4-dimethyl- N-((6-methyl-4-(methylthio)-2-oxo-l,2-dihydropyridin-3- yl)methyl)benzo[d][l,3]dioxole-5-carboxamide
• Step 1 Synthesis of methyl 7-chloro-2-(4-(3-(difluoromethoxy)azetidin-l- yl)cyclohexyl)-2,4-dimethylbenzo[d][l,3]dioxole-5-carboxylate
• a lithium borohydride solution in tetrahydrofuran (2 M, 500 pL, 1 mmol) was added dropwise at -78 °C before the reaction was allowed to warm to room temperature for 15 min. Next, the mixture was cooled to 0 °C then quenched with a saturated aqueous solution of sodium bicarbonate, diluted with dichloromethane and warmed to room temperature. The desired product was extracted from the aqueous layer with dichloromethane (thrice) and the combined organic layers dried using a hydrophobic filter and concentrated to dryness under reduced pressure.
• Step 2 Synthesis of 7-chloro-2-(4-(3-(difluoromethoxy)azetidin-l- yl)cyclohexyl)-2,4-dimethylbenzo[d][l,3]dioxole-5-carboxylic acid
• Step 3 Synthesis of perfluorophenyl 7-chloro-2-((lr,4R)-4-(3-
• Step 4 Synthesis of 7-chloro-2-(4-(3-(difluoromethoxy)azetidin-l- yl)cyclohexyl)-2,4-dimethyl-N-((6-methyl-4-(methylthio)-2-oxo-l,2-dihydropyridin-3- yl)methyl)benzo[d][l,3]dioxole-5-carboxamide
• Step 1 Synthesis of 7-chloro-2,4-dimethyl-2-(4-((3-(trifluoromethyl)oxetan-3- yl)amino)cyclohexyl)benzo[d][l,3]dioxole-5-carboxylic acid
• Step 2 Synthesis of perfluorophenyl 7-chloro-2,4-dimethyl-2-(4-((3- (trifluoromethyl)oxetan-3-yl)amino)cyclohexyl)benzo[d][l,3]dioxole-5-carboxylate
• Step 3 Synthesis of 7-chloro-2,4-dimethyl-N-((6-methyl-4-(methylthio)-2-oxo- l,2-dihydropyridin-3-yl)methyl)-2-(4-((3-(trifluoromethyl)oxetan-3- yl)amino)cyclohexyl)benzo[d][l,3]dioxole-5-carboxamide
• Example 8 7-chloro-N-((4-chloro-6-methyl-2-oxo-l,2-dihydropyridin-3-yl)methyl)-2-(4- (3-cyclopropoxyazetidin-l-yl)cyclohexyl)-2,4-dimethylbenzo[d][l,3]dioxole-5- carboxamide
• Step 1 Synthesis of methyl 7-chloro-2-(4-(3-cyclopropoxyazetidin-l- yl)cyclohexyl)-2,4-dimethylbenzo[d][l,3]dioxole-5-carboxylate
• reaction mixture was stirred at room temperature for 1.5 h then cooled to -70 °C.
• Lithium borohydride 120 mg, 5.51 mmol was added and the reaction stirred at -70 °C for 30 min [or until complete consumption of the starting material was observed by TLC, ethyl acetate/methanol 5:1].
• the reaction was quenched with a saturated aqueous solution of ammonium chloride (100 mL) and the desired product was extracted with ethyl acetate (50 mL x 2). The combined organic layers were washed with brine (30 mL), dried over sodium sulfate, filtered and concentrated to dryness under reduced pressure.
• Step 2 Synthesis of 7-chloro-2-(4-(3-cyclopropoxyazetidin-l-yl)cyclohexyl)-
• Step 3 Synthesis of 7-chloro-N-((4-chloro-6-methyl-2-oxo-l,2- dihydropyridin-3-yl)methyl)-2-(4-(3-cyclopropoxyazetidin-l-yl)cyclohexyl)-2,4- dimethylbenzo[d] [ 1 ,3 ]dioxole-5-carboxamide
• Example 14 (R)-7-chloro-2,4-dimethyl-N-((6-methyl-4-(methylthio)-2-oxo-l,2- dihydropyridin-3-yl)methyl)-2-(l-(2,2,2-trifluoroethyl)piperidin-4- yl)benzo[d] [l,3]dioxole-5-carboxamide and (S)-7-chloro-2,4-dimethyl-N-((6-methyl-4- (methylthio)-2-oxo-l,2-dihydropyridin-3-yl)methyl)-2-(l-(2,2,2-trifluoroethyl)piperidin-
• Step 1 Synthesis of 7-chloro-2,4-dimethyl-N-((6-methyl-4-(methylthio)-2-oxo- l,2-dihydropyridin-3-yl)methyl)-2-(l-(2,2,2-trifluoroethyl)piperidin-4- yl)benzo[d] [ 1 ,3] dioxole-5-carboxamide
• reaction mixture was heated at 60 °C and after 1 h added onto a C18 column for purification.
• desired product was purified twice by reverse phase flash chromatography (Cl 8 column, gradient 0 to 100% acetonitrile in water with 0.1% of trifluoroacetic acid) to give the title compound (289 mg, 38% yield) as a racemic mixture as a gum.
• Step 2 Separation of (R)-7-chloro-2,4-dimethyl-N-((6-methyl-4-(methylthio)- 2-oxo-l,2-dihydropyridin-3-yl)methyl)-2-(l-(2,2,2-trifluoroethyl)piperidin-4- yl)benzo[d][l,3]dioxole-5-carboxamide and (S)-7-chloro-2,4-dimethyl-N-((6-methyl-4- (methylthio)-2-oxo-l,2-dihydropyridin-3-yl)methyl)-2-(l-(2,2,2-trifluoroethyl)piperidin- 4-yl)benzo[d][l,3]dioxole-5-carboxamide
• Example 15 7-chloro-N-((4-chloro-6-methyl-2-oxo-l,2-dihydropyridin-3-yl)methyl)-2- (4-(3-methoxyazetidin-l-yl)cyclohexyl)-2,4-dimethylbenzo[d][l,3]dioxole-5-carboxamide
• Example 16 (R)-7-chloro-N-((4-chloro-6-methyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-2,4-dimethyl-2-(l-(2,2,2-trifluoroethyl)piperidin-4-yl)benzo[d][l,3]dioxole-5- carboxamide and (S)-7-chloro-N-((4-chloro-6-methyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-2,4-dimethyl-2-(l-(2,2,2-trifluoroethyl)piperidin-4-yl)benzo[d][l,3]dioxole-5- carboxamide
• Step 1 Synthesis of 7-chloro-N-((4-chloro-6-methyl-2-oxo-l,2- dihydropyridin-3-yl)methyl)-2,4-dimethyl-2-(l-(2,2,2-trifluoroethyl)piperidin-4- yl)benzo[d] [ 1 ,3] dioxole-5-carboxamide
• Step 2 Separation of (R)-7-chloro-N-((4-chloro-6-methyl-2-oxo-l,2- dihydropyridin-3-yl)methyl)-2,4-dimethyl-2-(l-(2,2,2-trifluoroethyl)piperidin-4- yl)benzo[d][l,3]dioxole-5-carboxamide and (S)-7-chloro-N-((4-chloro-6-methyl-2-oxo-
• Example 17 7-chloro-2-(4-(3-methoxyazetidin-l-yl)cyclohexyl)-2,4-dimethyl-N-((6- methyl-4-(methylthio)-2-oxo-l,2-dihydropyridin-3-yl)methyl)benzo[d][l,3]dioxole-5- carboxamide
• EZH2 biochemical assay (IC50): Compound potencies were assessed through incorporation of H-SAM into a biotinylated H3 peptide. Specifically, 30 pM PRC2 containing wt EZH2 (pentameric complex prepared in-house) was pre-incubated with 450 nM SAM, 450 nM 3 H-SAM, 2 mM H3K27me3 activating peptide (H 2 N- RKQLATKAAR(Kme3)SAPATGGVKKP-amide) and compounds (as 10 point duplicate dose response titrations in DMSO, final assay 0.8% DMSO (v/v)) for 3-5 h in 50 mM Tris (pH 8.5), 1 mM DTT, 0.07 mM Brij-35, 0.1% BSA, and 0.8% DMSO in a total volume of 12.5 m ⁇ .
• H3K27me3 Alpha Hela Assay (AlphaLISA).
• Ten different doses of each test compound (in a series of 3-fold dilutions) were plated in duplicate 384-well tissue culture treated plates (Catalog # 6007680; Perkin Elmer, Waltham, MA). Hela cells grown in culture were trypsinized and counted using a Countess® cell counter (Catalog # C 10281; Life Technologies, Grand Island, NY). Cell were diluted to 67,000 cells per mL inl0% DMEM (Catalog # 10569-010 Life Technologies, Grand Island, NY) and l5pL (1,000 cells) were plated into each well using the Biotek MicroFloTM Select Dispenser (BioTek Instruments,
• Biotinylated anti-Histone H3 (C-ter) Antibody (diluted to 3nM final) (Catalog #ALl l8 Perkin Elmer; Waltham, MA) was added in each well. Dilution of the acceptor beads and anti-Histone H3 was done in IX Histone Detection buffer (Catalog # AL009F3 Perkin Elmer; Waltham, MA) which was produced by diluting the 10X stock provided. The plate was sealed with an aluminum plate sealer and incubated at 23°C for 60 min.
• Cell viability was assayed by adding 15 pL of Cell Titer Glo ((Catalog #G924l Promega Madison, WI) to each well with cells with media. The plates were incubated at RT for 15 - 20 minutes on a plate shaker at low speed. The plates were then read using an EnVision- Alpha Reader (model # 2104 Perkin Elmer; Waltham, MA).
• EZH2 residence time assay Compound residence times were assessed by monitoring the recovery of enzyme activity following a lOO-fold dilution of pre-formed enzyme-inhibitor complex (dilution reaction), and comparing it to the activity of an undiluted control with the same final concentrations of all reagents (control reaction). Enzyme activity was measured by incorporation of H-SAM into a biotinylated H3 peptide.
• K is the inhibition constant
• [£i] is the calculated equilibrium concentration of enzyme-inhibitor complex
• [E] is the calculated equilibrium concentration of free enzyme
• [I] is the calculated equilibrium concentration of free inhibitor.
• MDCK II cells obtained from Piet Borst at the Netherlands Cancer Institute were seeded onto polyethylene membranes (PET) in 96-well BD insert systems at 2.5 x 105 cells/ mL until to 4-7 days for confluent cell monolayer formation.
• PET polyethylene membranes
• Test and reference compounds (nadolol, metoprolol and digoxin) were diluted with transport buffer (HBSS with lOmM Hepes, pH7.4) from stock solution to a concentration of 2 mM ( ⁇ l% DMSO) and applied to the apical (A) or basolateral (B) side of the cell monolayer. Permeation of the test compounds from A to B direction or B to A direction was determined in duplicate with P-gp inhibitor (GF120918, 10 pM).
• Digoxin was tested at 10 pM from A to B direction or B to A direction with/without P-gp inhibitor (GF120918, 10 pM) as well, while nadolol and metoprolol were tested at 2 pM in A to B direction without P-gp inhibitor (GF120918, 10 pM) in duplicate.
• the plate was incubated for 2.5 hours in C0 2 incubator at 37 ⁇ l°C, with 5% C0 2 at saturated humidity without shaking.
• the efflux ratio of each compound was also determined.
• Test and reference compounds were quantified by FC/MS/MS analysis based on the peak area ratio of analyte/IS. After transport assay, Fucifer yellow rejection assay are applied to determine the cell monolayer integrity.
• Buffers are removed from both apical and basolateral chambers, followed by the addition of 75 pF of 100 pM lucifer yellow in transport buffer and 250 pF transport buffer in apical and basolateral chambers, respectively.
• the plate is incubated for 30 minutes at 37°C with 5% C0 2 and 95% relative humidity without shaking. After 30 minutes incubation, 20 pF of lucifer yellow samples are taken from the apical sides, followed by the addition of 60 pL of Transport Buffer. And then 80 pL of lucifer yellow samples are taken from the basolateral sides.
• the relative fluorescence unit (RFU) of lucifer yellow is measured at 425/528 nm (excitation/emission) with a Molecular Device M2e plate reader.
• dC r /dt is the cumulative concentration of compound in the receiver chamber as a function of time (pM/s);
• V r is the solution volume in the receiver chamber (0.075 mL on the apical side, 0.25 mL on the basolateral side);
• A is the surface area for the transport, i.e.
• Co is the initial concentration in the donor chamber (pM).
• V d is the volume in the donor chambers (0.075 mL on the apical side, 0.25 mL on the basolateral side);
• C d and C r are the final concentrations of transport compound in donor and receiver chambers, respectively.
• RFU Apical and RFU Ramiat rai are the relative fluorescence unit values of lucifer yellow in the apical and basolateral wells, respectively;
• V Apicai and V Basoiaterai are the volume of apical and basolateral wells (0.075 mL and 0.25 mL), respectively. Percent lucifer yellow should be less than 2. Data is shown in Table 6.

Landscapes

• Chemical & Material Sciences (AREA)
• Organic Chemistry (AREA)
• Health & Medical Sciences (AREA)
• General Health & Medical Sciences (AREA)
• Veterinary Medicine (AREA)
• Medicinal Chemistry (AREA)
• Public Health (AREA)
• Pharmacology & Pharmacy (AREA)
• Life Sciences & Earth Sciences (AREA)
• Animal Behavior & Ethology (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• General Chemical & Material Sciences (AREA)
• Epidemiology (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
• Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
• Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
• Plural Heterocyclic Compounds (AREA)
• Enzymes And Modification Thereof (AREA)
• Pyridine Compounds (AREA)

Priority Applications (32)

Applications Claiming Priority (2)

Publications (1)

Family

ID=66429588

Family Applications (1)

Country Status (34)

Cited By (17)

Families Citing this family (3)

Citations (1)

Family Cites Families (129)

• 2019
  • 2019-04-17 CR CR20200553A patent/CR20200553A/es unknown
  • 2019-04-17 MY MYPI2020005336A patent/MY208662A/en unknown
  • 2019-04-17 MA MA52288A patent/MA52288B1/fr unknown
  • 2019-04-17 CN CN202510130368.8A patent/CN119954787A/zh active Pending
  • 2019-04-17 KR KR1020257012107A patent/KR20250073166A/ko active Pending
  • 2019-04-17 DK DK19722363.9T patent/DK3781561T3/da active
  • 2019-04-17 SG SG11202009438UA patent/SG11202009438UA/en unknown
  • 2019-04-17 SI SI201930767T patent/SI3781561T1/sl unknown
  • 2019-04-17 HR HRP20240793TT patent/HRP20240793T1/hr unknown
  • 2019-04-17 WO PCT/US2019/027932 patent/WO2019204490A1/en not_active Ceased
  • 2019-04-17 UA UAA202006100A patent/UA127357C2/uk unknown
  • 2019-04-17 LT LTEPPCT/US2019/027932T patent/LT3781561T/lt unknown
  • 2019-04-17 JP JP2020557175A patent/JP7256823B2/ja active Active
  • 2019-04-17 KR KR1020207032832A patent/KR102798145B1/ko active Active
  • 2019-04-17 EA EA202092490A patent/EA202092490A1/ru unknown
  • 2019-04-17 AU AU2019255310A patent/AU2019255310B2/en active Active
  • 2019-04-17 HU HUE19722363A patent/HUE066783T2/hu unknown
  • 2019-04-17 EP EP24160308.3A patent/EP4421072A1/en active Pending
  • 2019-04-17 MX MX2020010999A patent/MX2020010999A/es unknown
  • 2019-04-17 CN CN201980026504.1A patent/CN111989325B/zh active Active
  • 2019-04-17 SM SM20240233T patent/SMT202400233T1/it unknown
  • 2019-04-17 PL PL19722363.9T patent/PL3781561T3/pl unknown
  • 2019-04-17 AR ARP190101038A patent/AR114793A1/es unknown
  • 2019-04-17 TW TW108113388A patent/TWI828677B/zh active
  • 2019-04-17 MD MDE20210170T patent/MD3781561T2/ro unknown
  • 2019-04-17 PE PE2020001613A patent/PE20201448A1/es unknown
  • 2019-04-17 PT PT197223639T patent/PT3781561T/pt unknown
  • 2019-04-17 IL IL278013A patent/IL278013B2/en unknown
  • 2019-04-17 ES ES19722363T patent/ES2983344T3/es active Active
  • 2019-04-17 EP EP19722363.9A patent/EP3781561B1/en active Active
  • 2019-04-17 RS RS20240661A patent/RS65621B1/sr unknown
  • 2019-04-17 FI FIEP19722363.9T patent/FI3781561T3/fi active
  • 2019-04-17 BR BR112020021194-0A patent/BR112020021194A2/pt active Search and Examination
  • 2019-04-18 US US16/387,851 patent/US10689371B2/en active Active
• 2020
  • 2020-04-23 US US16/856,454 patent/US11274095B2/en active Active
  • 2020-09-25 PH PH12020551578A patent/PH12020551578A1/en unknown
  • 2020-10-16 CL CL2020002698A patent/CL2020002698A1/es unknown
  • 2020-11-17 CO CONC2020/0014217A patent/CO2020014217A2/es unknown
• 2023
  • 2023-03-31 JP JP2023057753A patent/JP2023082132A/ja active Pending

Patent Citations (1)

Non-Patent Citations (12)

Also Published As

Similar Documents

Legal Events