WO2011105721A3 - 항원고정화 면역형광 슬라이드의 제조방법 및 그에 의해 제조되는 면역형광 슬라이드 - Google Patents

항원고정화 면역형광 슬라이드의 제조방법 및 그에 의해 제조되는 면역형광 슬라이드 Download PDF

Info

Publication number
WO2011105721A3
WO2011105721A3 PCT/KR2011/001126 KR2011001126W WO2011105721A3 WO 2011105721 A3 WO2011105721 A3 WO 2011105721A3 KR 2011001126 W KR2011001126 W KR 2011001126W WO 2011105721 A3 WO2011105721 A3 WO 2011105721A3
Authority
WO
WIPO (PCT)
Prior art keywords
slide
antigen
prepared
immunofluorescence
modified
Prior art date
Application number
PCT/KR2011/001126
Other languages
English (en)
French (fr)
Other versions
WO2011105721A2 (ko
WO2011105721A9 (ko
Inventor
김남수
조용진
김종태
Original Assignee
한국식품연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한국식품연구원 filed Critical 한국식품연구원
Priority to US13/261,437 priority Critical patent/US20130029428A1/en
Priority to EP11747611.9A priority patent/EP2541248B1/en
Priority to CN201180019524XA priority patent/CN102859359A/zh
Publication of WO2011105721A2 publication Critical patent/WO2011105721A2/ko
Publication of WO2011105721A9 publication Critical patent/WO2011105721A9/ko
Publication of WO2011105721A3 publication Critical patent/WO2011105721A3/ko

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4737—C-reactive protein

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본 발명에 의하여, C-반응성 단백질을 슬라이드에 고정시켜 단백질 칩을 제조하는 단계, 분석하고자 하는 단백질에 특이적으로 결합하는 항체를 스트렙트아비딘과 혼합하여 형광나노입자로 표지시키는 단계, 상기 항체를 혼합경쟁적으로 면역반응시키는 단계, 형광카메라로 분석하는 단계에 있어서, 상기 C-반응성 단백질을 슬라이드에 고정화하기 위하여, 3-아미노프로필트리메톡시실란으로 개질된 슬라이드를 제조하는 단계, 3-아미노프로필트리메톡시실란으로 개질된 슬라이드를 수화하는 단계, 글루타르 알데히드 용액을 사용하여 상기 개질된 슬라이드를 활성화시키는 단계, 30-70mM 인산버퍼용액(pH 6.5-7.8)에 C-반응성 단백을 0.01-0.5㎎/㎖ 농도로 용해하여 고정화용 항원용액을 제조하는 단계, 스포팅 가이드 상에 상기 슬라이드를 포함하는 페트리디쉬를 올려놓고 상기 제조한 항원용액을 1-100㎕ 적하점에 스포팅하는 단계, 및 상기 단계에 의하여 준비된 슬라이드를 1-6시간 반응시켜 항원을 고정화하는 항원고정화 면역형광 슬라이드의 제조방법 및 이에 의하여 제조되는 면역형광 슬라이드가 개시된다.
PCT/KR2011/001126 2010-02-23 2011-02-21 항원고정화 면역형광 슬라이드의 제조방법 및 그에 의해 제조되는 면역형광 슬라이드 WO2011105721A2 (ko)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US13/261,437 US20130029428A1 (en) 2010-02-23 2011-02-21 Preparation method of antigen-immobilized immuno- fluorescence slide and immuno-fluoroscence slide prepared thereby
EP11747611.9A EP2541248B1 (en) 2010-02-23 2011-02-21 Preparation method of antigen-immobilized immunofluorescence slide, and immunofluorescence slide prepared thereby
CN201180019524XA CN102859359A (zh) 2010-02-23 2011-02-21 固定有抗原的免疫荧光载片的制备方法和由该方法制备的免疫荧光载片

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020100016157A KR101140029B1 (ko) 2010-02-23 2010-02-23 항원고정화 면역형광 슬라이드의 제조방법 및 그에 의해 제조되는 면역형광 슬라이드
KR10-2010-0016157 2010-02-23

Publications (3)

Publication Number Publication Date
WO2011105721A2 WO2011105721A2 (ko) 2011-09-01
WO2011105721A9 WO2011105721A9 (ko) 2012-04-19
WO2011105721A3 true WO2011105721A3 (ko) 2012-06-07

Family

ID=44507349

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/001126 WO2011105721A2 (ko) 2010-02-23 2011-02-21 항원고정화 면역형광 슬라이드의 제조방법 및 그에 의해 제조되는 면역형광 슬라이드

Country Status (5)

Country Link
US (1) US20130029428A1 (ko)
EP (1) EP2541248B1 (ko)
KR (1) KR101140029B1 (ko)
CN (4) CN104316698A (ko)
WO (1) WO2011105721A2 (ko)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011077838A1 (ja) * 2009-12-25 2011-06-30 コニカミノルタエムジー株式会社 蛍光物質内包シリカナノ粒子及び生体物質標識剤
CA2842321C (en) 2011-07-18 2022-05-03 President And Fellows Of Harvard College Engineered microbe-targeting molecules and uses thereof
US10551379B2 (en) 2013-03-15 2020-02-04 President And Fellows Of Harvard College Methods and compositions for improving detection and/or capture of a target entity
CA2913155A1 (en) 2013-05-21 2014-11-27 President And Fellows Of Harvard College Engineered heme-binding compositions and uses thereof
CN105723220A (zh) * 2013-11-12 2016-06-29 光州科学技术院 能够测定广泛浓度范围的生物物质浓度的免疫层析条状传感器
EP3083658B1 (en) 2013-12-18 2019-05-08 President and Fellows of Harvard College Crp capture/detection of gram positive bacteria
US20160337327A1 (en) * 2013-12-30 2016-11-17 Telecom Italia S.P.A. Method for managing a node association in a wireless personal area communication network
CN103941017B (zh) * 2014-03-18 2016-01-20 北京普恩光德生物科技开发有限公司 C反应蛋白检测试剂盒
US20170342470A1 (en) * 2014-12-12 2017-11-30 Konica Minolta, Inc. Diluent for fluorescent nano particles, kit for immunofluorescent staining which utilizes same, solution for immunofluorescent staining, immunofluorescent staining method, and gene staining method
CN104678105A (zh) * 2015-01-16 2015-06-03 镇江市第一人民医院 检测Cripto-1的酶联免疫试剂盒及其制备方法
CN107430119A (zh) * 2015-03-10 2017-12-01 西门子医疗保健诊断公司 固体载体上的官能团的定量
TWI547693B (zh) * 2015-05-07 2016-09-01 希華晶體科技股份有限公司 感測方法及感測晶片
EP3763378A1 (en) 2015-08-06 2021-01-13 President and Fellows of Harvard College Improved microbe-binding molecules and uses thereof
CN106093427B (zh) * 2016-06-02 2018-04-13 滨州医学院 同时鉴定弓形虫、风疹病毒和单纯疱疹病毒的新方法
CN106896094B (zh) * 2017-04-11 2023-07-14 中国农业科学院饲料研究所 一种同时检测cle、rac和sbl的方法及其专用纸芯片
CN109900895A (zh) * 2017-12-11 2019-06-18 北京和杰创新生物医学科技有限公司 细胞爬片用玻璃薄片表面的粘附处理方法
CN110763834B (zh) * 2018-07-25 2023-06-30 福建广生堂药业股份有限公司 一种检测免疫标志物含量的方法、试剂和试剂盒
CN108918856B (zh) * 2018-07-31 2021-11-09 济南大学 一种双MOFs材料猝灭型电致化学发光免疫传感器的制备方法及应用
CN109490525B (zh) * 2018-11-08 2021-09-07 南京光声超构材料研究院有限公司 一种溴化银膜sers免疫基底的制备方法
CN109633174B (zh) * 2019-01-09 2022-04-26 昆明医科大学海源学院 一种基于生物素量子点探针的c反应蛋白超敏检测方法
CN111686826B (zh) * 2019-03-15 2023-05-23 国家纳米科学中心 分层结构的微流控芯片及其应用
CN110333341A (zh) * 2019-07-04 2019-10-15 浙江理工大学 一种基于蛋白质芯片技术鉴别丝织品文物样的方法
CN110646607A (zh) * 2019-07-12 2020-01-03 广东工业大学 一种介孔二氧化硅包裹的正电荷纳米金偶联抗体及其制备方法和应用
CN111024468A (zh) * 2019-12-20 2020-04-17 天津金域医学检验实验室有限公司 一种呼吸道病原体免疫荧光检测中样本的处理方法
CN113030479B (zh) * 2019-12-25 2023-09-12 洛阳中科生物芯片技术有限公司 一种蛋白质芯片的点样溶液
CN111208295A (zh) * 2020-02-29 2020-05-29 济南德亨医学科技有限公司 一种阴道加德纳菌量子点免疫检测方法
CN111208288A (zh) * 2020-02-29 2020-05-29 济南德亨医学科技有限公司 一种阴道毛滴虫量子点荧光免疫检测方法
CN111266141B (zh) * 2020-03-19 2022-07-08 京东方科技集团股份有限公司 一种检测芯片及其修饰方法
CN112014369B (zh) * 2020-08-25 2024-02-09 上海市皮肤病医院 超灵敏数字层析快速检测分析物的系统及方法
CN112986580B (zh) * 2021-02-10 2024-03-01 天津中新科炬生物制药股份有限公司 一种新型冠状病毒中和抗体检测方法及试剂盒
CN113567660A (zh) * 2021-07-22 2021-10-29 中南大学湘雅二医院 一种检测系统性红斑狼疮患者自身抗体的方法
CN115754281B (zh) * 2022-11-28 2023-08-15 广东省大湾区华南理工大学聚集诱导发光高等研究院 一种荧光纳米粒子在免疫荧光组化染色中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100620959B1 (ko) * 2005-06-30 2006-09-08 서강대학교산학협력단 형광물질을 내포한 제올라이트를 포함하는 표지 및 이의용도
US20070048807A1 (en) * 2005-08-31 2007-03-01 Kimberly-Clark Worldwide, Inc. Diagnostic test kits with improved detection accuracy

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61272661A (ja) * 1985-05-29 1986-12-02 Hitachi Ltd イムノアツセイ法
JP3493543B2 (ja) * 1998-04-28 2004-02-03 大塚製薬株式会社 抗体測定方法
KR20030038932A (ko) 2001-11-09 2003-05-17 바이오인포메틱스 주식회사 회전코팅법을 이용한 고체표면기질 상의 균일한 고분자층형성방법
KR101071430B1 (ko) * 2002-08-27 2011-10-10 킴벌리-클라크 월드와이드, 인크. 시분해 형광을 이용한 막 기반 분석법
CN1298807C (zh) * 2002-11-01 2007-02-07 中国科学院大连化学物理研究所 一种功能性纳米稀土荧光微粒及其制备和应用
CN1173182C (zh) * 2002-12-18 2004-10-27 陕西超英生物医学研究开发有限公司 糖尿病自身免疫抗体检测蛋白芯片、其制备及检测方法
CN1781020A (zh) * 2003-04-25 2006-05-31 捷时雅株式会社 生物芯片和生物芯片试剂盒,及其制造方法和使用方法
CN1265197C (zh) * 2003-07-04 2006-07-19 中国科学院大连化学物理研究所 β－二酮－三价铕配合物纳米荧光探针及其制备和应用
CN1588070A (zh) * 2004-09-06 2005-03-02 南开大学 多组分生物微阵列芯片的制备方法
CN101008644A (zh) * 2006-01-27 2007-08-01 陈云 功能性中枢神经损伤诊断检测用蛋白芯片及其制造方法
WO2009009188A2 (en) * 2007-04-19 2009-01-15 3M Innovative Properties Company Uses of water-dispersible silica nanoparticles for attaching biomolecules
KR100921237B1 (ko) * 2007-12-31 2009-10-12 한국식품연구원 Ｃ-반응성 단백 측정용 수정진동자 면역센서 측정장치 및수정진동자 면역센서를 이용한 ｃ-반응성 단백의 고감도측정방법
CN101216491B (zh) * 2008-01-08 2012-06-06 广州益善生物技术有限公司 结核分枝杆菌检测液相芯片及其制备方法
WO2009134822A2 (en) * 2008-05-01 2009-11-05 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Fluorescent organic nanoparticles
CN100585388C (zh) * 2008-05-26 2010-01-27 湖南大学 采用双色流式细胞术检测结核杆菌的方法
CN101526535B (zh) * 2009-04-14 2011-04-20 河南省豫康生物工程技术有限公司 多种肿瘤标志物联合检测液相芯片及其制备方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100620959B1 (ko) * 2005-06-30 2006-09-08 서강대학교산학협력단 형광물질을 내포한 제올라이트를 포함하는 표지 및 이의용도
US20070048807A1 (en) * 2005-08-31 2007-03-01 Kimberly-Clark Worldwide, Inc. Diagnostic test kits with improved detection accuracy

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JUNG Y ET AL.: "Controlled antibody immobilization onto immunoanalytical platforms by synthetic peptide", ANAL BIOCHEM., vol. 374, no. 1, 1 March 2008 (2008-03-01), pages 99 - 105, XP022437940 *
LEE, YOONSUK ET AL.: "Proteochip: A highly sensitive protein microarray prepared by a novel method of protein immobilization for application of protein-protein interaction studies", PROTEOMICS, vol. 3, March 2003 (2003-03-01), pages 2289 - 2304, XP008057185 *
See also references of EP2541248A4 *

Also Published As

Publication number Publication date
CN104316697A (zh) 2015-01-28
KR20110096739A (ko) 2011-08-31
CN104316678A (zh) 2015-01-28
EP2541248B1 (en) 2017-07-12
US20130029428A1 (en) 2013-01-31
WO2011105721A2 (ko) 2011-09-01
EP2541248A4 (en) 2013-09-04
CN102859359A (zh) 2013-01-02
EP2541248A2 (en) 2013-01-02
WO2011105721A9 (ko) 2012-04-19
CN104316698A (zh) 2015-01-28
KR101140029B1 (ko) 2012-06-21

Similar Documents

Publication Publication Date Title
WO2011105721A3 (ko) 항원고정화 면역형광 슬라이드의 제조방법 및 그에 의해 제조되는 면역형광 슬라이드
TW200604527A (en) An immunological method and reagent for determing the inhibited nonspecific reaction
MX349562B (es) Ensayo de inmuno deteccion multiplex.
US20140011255A1 (en) Biotin Derivatives
RU2012141513A (ru) Иммунологический анализ на свободный витамин d
US11009505B2 (en) Reagent diluent
TW201233686A (en) Monoclonal antibody recognizing human papillomavirus (HPV) L2 protein and method for measuring HPV-neutralizing antibody titer using the same
CN101952729A (zh) 利用全细胞捕获和atp分析进行样品细菌分析的方法
JP2012505402A5 (ko)
EP2705031B1 (en) Flash and glow 1,2-dioxetanes
TW201802472A (zh) 抗人類血紅素單株抗體或抗體套組、抗人類血紅素單株抗體固定化不可溶性載體粒子、及使用其等之測定試劑或測定方法
WO2009008414A1 (ja) Ｍｍｐ１３に対する中和活性を有するモノクローナル抗体
CN102341707B (zh) 检测试剂以及使用该检测试剂测定检测样品中的被测定物的方法
CN105758846A (zh) 检测克伦特罗的化学发光酶联免疫检测试剂盒
CN103197077A (zh) 一种检测微量牛免疫球蛋白g的试剂盒
EP2660603B1 (en) Immunological assay method
CN102980884A (zh) 胃黏膜样品中幽门螺旋杆菌的化学发光免疫测定法
JP5997446B2 (ja) 甲状腺ホルモン固定化担体の液体試薬およびその用途
CN203732548U (zh) 一种维生素d抗原包被的反应板
JP4260872B1 (ja) 高感度酵素免疫測定法
Spudich et al. Attachment of anti-GFP antibodies to microspheres for optical trapping experiments
JP2011122957A (ja) 高特異的かつ高感度なタンパク質検出方法
RU2377299C1 (ru) ШТАММ 5А10 ПОСТОЯННОЙ ГИБРИДОМНОЙ ЛИНИИ КЛЕТОК МЫШИ Mus. musculus - ПРОДУЦЕНТ МОНОКЛОНАЛЬНЫХ АНТИТЕЛ К IgG КРУПНОГО РОГАТОГО СКОТА
US20130203085A1 (en) Antigenic structure and uses thereof for screening trypanosomiases in humans and animals
JP2017173113A (ja) ＴＲＡｂ測定試薬およびその製造方法

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 201180019524.X

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11747611

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2012554897

Country of ref document: JP

REEP Request for entry into the european phase

Ref document number: 2011747611

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 13261437

Country of ref document: US

Ref document number: 2011747611

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP