WO2008117735A1 - 高純度可溶性トロンボモジュリンの製造方法 - Google Patents
高純度可溶性トロンボモジュリンの製造方法 Download PDFInfo
- Publication number
- WO2008117735A1 WO2008117735A1 PCT/JP2008/055211 JP2008055211W WO2008117735A1 WO 2008117735 A1 WO2008117735 A1 WO 2008117735A1 JP 2008055211 W JP2008055211 W JP 2008055211W WO 2008117735 A1 WO2008117735 A1 WO 2008117735A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- soluble thrombomodulin
- variant
- high purity
- substantially contains
- producing soluble
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/7455—Thrombomodulin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Abstract
Priority Applications (11)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP08722575.1A EP2138505B1 (en) | 2007-03-23 | 2008-03-21 | Method for producing soluble thrombomodulin of high purity |
AU2008230465A AU2008230465B2 (en) | 2007-03-23 | 2008-03-21 | Method for producing high-purity soluble thrombomodulin |
ES08722575.1T ES2496596T3 (es) | 2007-03-23 | 2008-03-21 | Método para producir trombomodulina soluble de gran pureza |
NZ579873A NZ579873A (en) | 2007-03-23 | 2008-03-21 | Method for producing soluble thrombomodulin of high purity |
JP2009506314A JP5117486B2 (ja) | 2007-03-23 | 2008-03-21 | 高純度可溶性トロンボモジュリンの製造方法 |
KR1020097017230A KR101238047B1 (ko) | 2007-03-23 | 2008-03-21 | 고순도 가용성 트롬보모듈린의 제조방법 |
CA2681294A CA2681294C (en) | 2007-03-23 | 2008-03-21 | Method for producing high-purity soluble thrombomodulin |
US12/532,598 US8258269B2 (en) | 2007-03-23 | 2008-03-21 | Method for producing high-purity soluble thrombomodulin |
CN2008800096257A CN101641368B (zh) | 2007-03-23 | 2008-03-21 | 高纯度可溶性血栓调节蛋白的制造方法 |
HK10104518.3A HK1137185A1 (en) | 2007-03-23 | 2010-05-10 | Method for producing soluble thrombomodulin of high purity |
US13/557,645 US8952137B2 (en) | 2007-03-23 | 2012-07-25 | Method for producing high-purity soluble thrombomodulin |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007077389 | 2007-03-23 | ||
JP2007-077389 | 2007-03-23 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12/532,598 A-371-Of-International US8258269B2 (en) | 2007-03-23 | 2008-03-21 | Method for producing high-purity soluble thrombomodulin |
US13/557,645 Division US8952137B2 (en) | 2007-03-23 | 2012-07-25 | Method for producing high-purity soluble thrombomodulin |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2008117735A1 true WO2008117735A1 (ja) | 2008-10-02 |
Family
ID=39788470
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2008/055211 WO2008117735A1 (ja) | 2007-03-23 | 2008-03-21 | 高純度可溶性トロンボモジュリンの製造方法 |
Country Status (13)
Country | Link |
---|---|
US (2) | US8258269B2 (ja) |
EP (1) | EP2138505B1 (ja) |
JP (1) | JP5117486B2 (ja) |
KR (1) | KR101238047B1 (ja) |
CN (1) | CN101641368B (ja) |
AU (1) | AU2008230465B2 (ja) |
CA (1) | CA2681294C (ja) |
ES (1) | ES2496596T3 (ja) |
HK (1) | HK1137185A1 (ja) |
NZ (1) | NZ579873A (ja) |
SG (1) | SG184699A1 (ja) |
TW (2) | TWI461434B (ja) |
WO (1) | WO2008117735A1 (ja) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011136313A1 (ja) | 2010-04-30 | 2011-11-03 | 旭化成ファーマ株式会社 | 高純度可溶性トロンボモジュリン及びその製造方法 |
US20120238727A1 (en) * | 2011-03-14 | 2012-09-20 | Catalent Pharma Solutions Llc | Decorin compositions and use thereof |
JP2013517284A (ja) * | 2010-01-15 | 2013-05-16 | バイオ−ラッド ラボラトリーズ インコーポレーティッド | アパタイトの表面中和方法 |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8895707B2 (en) | 2010-08-18 | 2014-11-25 | Bio-Rad Laboratories, Inc. | Elution of proteins from hydroxyapatite resins without resin deterioration |
CA2825651C (en) | 2011-02-02 | 2020-03-24 | Bio-Rad Laboratories, Inc. | Apatite surface neutralization with alkali solutions |
KR101672108B1 (ko) | 2011-11-15 | 2016-11-02 | 아사히 가세이 파마 가부시키가이샤 | 패혈증의 치료 및/또는 개선을 위한 의약 |
WO2013180933A1 (en) | 2012-05-30 | 2013-12-05 | Bio-Rad Laboratories, Inc. | In situ restoration of apatite-based chromatography resins |
US9802822B2 (en) | 2014-06-23 | 2017-10-31 | Bio-Rad Laboratories, Inc. | Apatite pretreatment |
WO2015200299A2 (en) | 2014-06-23 | 2015-12-30 | Bio-Rad Laboratories, Inc. | Apatite in-situ restoration |
Citations (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1988009811A1 (en) | 1987-06-12 | 1988-12-15 | Novo-Nordisk A/S | Proteins and derivatives thereof |
JPS646219U (ja) | 1987-06-30 | 1989-01-13 | ||
JPH02255699A (ja) | 1989-03-28 | 1990-10-16 | Asahi Chem Ind Co Ltd | 新規血液抗凝固物質及びその製法 |
JPH037785B2 (ja) | 1985-11-26 | 1991-02-04 | Kajima Corp | |
JPH0386900A (ja) | 1988-08-29 | 1991-04-11 | Kowa Co | 新規なトロンビン結合性物質及びその製法 |
JPH03133380A (ja) | 1989-08-11 | 1991-06-06 | Eli Lilly & Co | ヒトトロンボモジュリン誘導体 |
JPH03218399A (ja) | 1988-12-27 | 1991-09-25 | Mochida Pharmaceut Co Ltd | 尿由来の抗血液疑固物質、その製法およびそれを含有する医薬組成物 |
JPH03259084A (ja) | 1990-03-06 | 1991-11-19 | Kowa Co | トロンビン結合性物質の製造法 |
WO1992000325A1 (en) | 1990-06-27 | 1992-01-09 | Mochida Pharmaceutical Co., Ltd. | Anticoagulant polypeptides |
WO1992003149A1 (en) | 1990-08-15 | 1992-03-05 | Berlex Laboratories, Inc. | Superior thrombomodulin analogs for pharmaceutical use |
JPH04210700A (ja) | 1990-12-12 | 1992-07-31 | Dai Ichi Seiyaku Co Ltd | 組換ヒトトロンボモジュリン誘導体 |
WO1993015755A1 (en) | 1992-02-05 | 1993-08-19 | Schering Aktiengesellschaft | Protease-resistant thrombomodulin analogs |
JPH05213998A (ja) | 1990-08-03 | 1993-08-24 | Asahi Chem Ind Co Ltd | 新規なポリペプチド及びこれを有効成分とする 医薬組成物 |
JPH05310787A (ja) | 1992-05-01 | 1993-11-22 | Asahi Chem Ind Co Ltd | 新規なポリペプチド |
JPH06321085A (ja) | 1993-05-18 | 1994-11-22 | Mazda Motor Corp | 車両のスリップ制御装置 |
JPH07155176A (ja) | 1994-05-31 | 1995-06-20 | Asahi Chem Ind Co Ltd | トロンビンによるプロテインcの活性化を促進する作用を有するペプチドを含有する培養物 |
WO1995016460A1 (fr) | 1993-12-17 | 1995-06-22 | Mochida Pharmaceutical Co., Ltd. | Dispositif a semi-conducteur |
JPH09110900A (ja) * | 1995-10-24 | 1997-04-28 | Nippon Chem Res Kk | トロンボモジュリンの精製方法 |
WO1999018994A1 (fr) * | 1997-10-15 | 1999-04-22 | Asahi Kasei Kogyo Kabushiki Kaisha | Procede pour conserver la qualite d'une solution parenterale aqueuse de thrombomoduline pour l'entreposage ou la distribution |
JPH11171790A (ja) | 1994-03-15 | 1999-06-29 | Asahi Chem Ind Co Ltd | トロンボモジュリン用変性防止剤 |
JPH11341990A (ja) | 1998-03-30 | 1999-12-14 | Asahi Chem Ind Co Ltd | 高純度可溶性トロンボモジュリンの製造方法 |
WO2003061687A1 (en) * | 2002-01-18 | 2003-07-31 | Asahi Kasei Pharma Corporation | High-concentration preparation of soluble thrombomodulin |
JP3822383B2 (ja) | 1993-12-17 | 2006-09-20 | 持田製薬株式会社 | 可溶性トロンボモジュリン含有組成物 |
Family Cites Families (9)
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JP2738428B2 (ja) | 1987-01-08 | 1998-04-08 | 旭化成工業株式会社 | トロンビンによるプロテインcの活性化を促進する作用を有するペプチド |
EP0816495A1 (en) * | 1987-01-08 | 1998-01-07 | Asahi Kasei Kogyo Kabushiki Kaisha | DNA coding for a peptide promoting the activation of protein C by thrombin and dprocess for producing the same |
US5202421A (en) * | 1988-12-27 | 1993-04-13 | Mochida Pharmaceutical Co., Ltd. | Anticoagulant substance obtained from urine and process for the preparation thereof |
US6063763A (en) * | 1989-04-28 | 2000-05-16 | Schering Aktiengesellschaft | Protease-resistant thrombomodulin analogs |
US5466668A (en) * | 1989-04-28 | 1995-11-14 | Schering Aktiengesellschaft | Superior thrombomodulin analogs for pharmaceutical use |
KR930008093B1 (ko) | 1990-08-03 | 1993-08-25 | 아사히가세이고오교 가부시끼가이샤 | 신규 폴리펩티드 및 이를 유효성분으로 하는 의약조성물 |
JP3007785B2 (ja) | 1993-03-16 | 2000-02-07 | 旭化成工業株式会社 | トロンボモジュリン組成物およびその変性防止方法 |
JPH07173189A (ja) | 1993-12-17 | 1995-07-11 | Masashi Kosakai | リガンド存在下で二段階に分けて行うヒドロキシアパタイトクロマトグラフィーの用法 |
JP2002517406A (ja) * | 1998-06-01 | 2002-06-18 | ジェネンテック・インコーポレーテッド | イオン交換クロマトグラフィーの使用による、凝集体からのタンパク質モノマーの分離 |
-
2008
- 2008-03-21 NZ NZ579873A patent/NZ579873A/en unknown
- 2008-03-21 US US12/532,598 patent/US8258269B2/en active Active
- 2008-03-21 ES ES08722575.1T patent/ES2496596T3/es active Active
- 2008-03-21 WO PCT/JP2008/055211 patent/WO2008117735A1/ja active Application Filing
- 2008-03-21 EP EP08722575.1A patent/EP2138505B1/en active Active
- 2008-03-21 AU AU2008230465A patent/AU2008230465B2/en active Active
- 2008-03-21 CN CN2008800096257A patent/CN101641368B/zh active Active
- 2008-03-21 JP JP2009506314A patent/JP5117486B2/ja active Active
- 2008-03-21 KR KR1020097017230A patent/KR101238047B1/ko active IP Right Grant
- 2008-03-21 CA CA2681294A patent/CA2681294C/en active Active
- 2008-03-21 TW TW097110165A patent/TWI461434B/zh active
- 2008-03-21 TW TW103115744A patent/TW201431872A/zh unknown
- 2008-03-21 SG SG2012042321A patent/SG184699A1/en unknown
-
2010
- 2010-05-10 HK HK10104518.3A patent/HK1137185A1/xx unknown
-
2012
- 2012-07-25 US US13/557,645 patent/US8952137B2/en active Active
Patent Citations (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH037785B2 (ja) | 1985-11-26 | 1991-02-04 | Kajima Corp | |
WO1988009811A1 (en) | 1987-06-12 | 1988-12-15 | Novo-Nordisk A/S | Proteins and derivatives thereof |
JPS646219U (ja) | 1987-06-30 | 1989-01-13 | ||
JPH0386900A (ja) | 1988-08-29 | 1991-04-11 | Kowa Co | 新規なトロンビン結合性物質及びその製法 |
JPH03218399A (ja) | 1988-12-27 | 1991-09-25 | Mochida Pharmaceut Co Ltd | 尿由来の抗血液疑固物質、その製法およびそれを含有する医薬組成物 |
JPH02255699A (ja) | 1989-03-28 | 1990-10-16 | Asahi Chem Ind Co Ltd | 新規血液抗凝固物質及びその製法 |
JPH03133380A (ja) | 1989-08-11 | 1991-06-06 | Eli Lilly & Co | ヒトトロンボモジュリン誘導体 |
JPH03259084A (ja) | 1990-03-06 | 1991-11-19 | Kowa Co | トロンビン結合性物質の製造法 |
WO1992000325A1 (en) | 1990-06-27 | 1992-01-09 | Mochida Pharmaceutical Co., Ltd. | Anticoagulant polypeptides |
JPH05213998A (ja) | 1990-08-03 | 1993-08-24 | Asahi Chem Ind Co Ltd | 新規なポリペプチド及びこれを有効成分とする 医薬組成物 |
WO1992003149A1 (en) | 1990-08-15 | 1992-03-05 | Berlex Laboratories, Inc. | Superior thrombomodulin analogs for pharmaceutical use |
JPH04210700A (ja) | 1990-12-12 | 1992-07-31 | Dai Ichi Seiyaku Co Ltd | 組換ヒトトロンボモジュリン誘導体 |
WO1993015755A1 (en) | 1992-02-05 | 1993-08-19 | Schering Aktiengesellschaft | Protease-resistant thrombomodulin analogs |
JPH05310787A (ja) | 1992-05-01 | 1993-11-22 | Asahi Chem Ind Co Ltd | 新規なポリペプチド |
JPH06321085A (ja) | 1993-05-18 | 1994-11-22 | Mazda Motor Corp | 車両のスリップ制御装置 |
WO1995016460A1 (fr) | 1993-12-17 | 1995-06-22 | Mochida Pharmaceutical Co., Ltd. | Dispositif a semi-conducteur |
JP3822383B2 (ja) | 1993-12-17 | 2006-09-20 | 持田製薬株式会社 | 可溶性トロンボモジュリン含有組成物 |
JPH11171790A (ja) | 1994-03-15 | 1999-06-29 | Asahi Chem Ind Co Ltd | トロンボモジュリン用変性防止剤 |
JPH07155176A (ja) | 1994-05-31 | 1995-06-20 | Asahi Chem Ind Co Ltd | トロンビンによるプロテインcの活性化を促進する作用を有するペプチドを含有する培養物 |
JPH09110900A (ja) * | 1995-10-24 | 1997-04-28 | Nippon Chem Res Kk | トロンボモジュリンの精製方法 |
JP3745805B2 (ja) | 1995-10-24 | 2006-02-15 | 日本ケミカルリサーチ株式会社 | トロンボモジュリンの精製方法 |
WO1999018994A1 (fr) * | 1997-10-15 | 1999-04-22 | Asahi Kasei Kogyo Kabushiki Kaisha | Procede pour conserver la qualite d'une solution parenterale aqueuse de thrombomoduline pour l'entreposage ou la distribution |
JPH11341990A (ja) | 1998-03-30 | 1999-12-14 | Asahi Chem Ind Co Ltd | 高純度可溶性トロンボモジュリンの製造方法 |
WO2003061687A1 (en) * | 2002-01-18 | 2003-07-31 | Asahi Kasei Pharma Corporation | High-concentration preparation of soluble thrombomodulin |
Non-Patent Citations (10)
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"Method in Enzymology", vol. 100, 1983, ACADEMIC PRESS, pages: 468 |
D. Z. WEN ET AL., BIOCHEMISTRY, vol. 26, 1987, pages 4350 - 4357 |
J. BIOL. CHEM., vol. 264, 1989, pages 10351 - 10353 |
K. GOMI ET AL., BLOOD, vol. 75, 1990, pages 1396 - 1399 |
KOJI SUZUKI, IGAKU NO AYUMI, vol. 125, 1983, pages 901 |
M. ZUSHI ET AL., J. BIOL. CHEM., vol. 246, 1989, pages 10351 - 10353 |
P. M. HOWLEY ET AL.: "Methods in Emzymology", vol. 101, 1983, ACADEMIC PRESS, pages: 387 |
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See also references of EP2138505A4 * |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2013517284A (ja) * | 2010-01-15 | 2013-05-16 | バイオ−ラッド ラボラトリーズ インコーポレーティッド | アパタイトの表面中和方法 |
US9914749B2 (en) | 2010-01-15 | 2018-03-13 | Bio-Rad Laboratories, Inc. | Surface neutralization of apatite |
US10676502B2 (en) | 2010-01-15 | 2020-06-09 | Bio-Rad Laboratories, Inc. | Surface neutralization of apatite |
WO2011136313A1 (ja) | 2010-04-30 | 2011-11-03 | 旭化成ファーマ株式会社 | 高純度可溶性トロンボモジュリン及びその製造方法 |
US20130143790A1 (en) * | 2010-04-30 | 2013-06-06 | Asahi Kasei Pharma Corporation | Highly-purified soluble thrombomodulin and method for producing same |
AU2011246021B2 (en) * | 2010-04-30 | 2014-03-13 | Asahi Kasei Pharma Corporation | High-purity soluble thrombomodulin and method for producing same |
JP2015071646A (ja) * | 2010-04-30 | 2015-04-16 | 旭化成ファーマ株式会社 | 高純度可溶性トロンボモジュリン |
JP5722314B2 (ja) * | 2010-04-30 | 2015-05-20 | 旭化成ファーマ株式会社 | 高純度可溶性トロンボモジュリン及びその製造方法 |
US9127089B2 (en) | 2010-04-30 | 2015-09-08 | Asahi Kasei Pharma Corporation | Highly-purified soluble thrombomodulin and method for producing same |
EP3150628A1 (en) | 2010-04-30 | 2017-04-05 | Asahi Kasei Pharma Corporation | Highly-purified soluble thrombomodulin |
US20120238727A1 (en) * | 2011-03-14 | 2012-09-20 | Catalent Pharma Solutions Llc | Decorin compositions and use thereof |
US20220073579A1 (en) * | 2011-03-14 | 2022-03-10 | Catalent Pharma Solutions Llc | Decorin compositions and use thereof |
Also Published As
Publication number | Publication date |
---|---|
TW200906848A (en) | 2009-02-16 |
CA2681294A1 (en) | 2008-10-02 |
ES2496596T3 (es) | 2014-09-19 |
KR101238047B1 (ko) | 2013-02-27 |
HK1137185A1 (en) | 2010-07-23 |
AU2008230465B2 (en) | 2011-10-13 |
EP2138505B1 (en) | 2014-08-06 |
US8258269B2 (en) | 2012-09-04 |
JPWO2008117735A1 (ja) | 2010-07-15 |
EP2138505A4 (en) | 2011-02-09 |
SG184699A1 (en) | 2012-10-30 |
TW201431872A (zh) | 2014-08-16 |
CN101641368B (zh) | 2013-05-08 |
CA2681294C (en) | 2014-05-06 |
US20130237693A1 (en) | 2013-09-12 |
AU2008230465A1 (en) | 2008-10-02 |
KR20090112709A (ko) | 2009-10-28 |
NZ579873A (en) | 2011-11-25 |
JP5117486B2 (ja) | 2013-01-16 |
US8952137B2 (en) | 2015-02-10 |
US20100145020A1 (en) | 2010-06-10 |
CN101641368A (zh) | 2010-02-03 |
TWI461434B (zh) | 2014-11-21 |
EP2138505A1 (en) | 2009-12-30 |
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