WO2003010302A1 - Cultured cell construct containing spheroids of cultured animal cells and utilization thereof - Google Patents

Cultured cell construct containing spheroids of cultured animal cells and utilization thereof Download PDF

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Publication number
WO2003010302A1
WO2003010302A1 PCT/JP2002/007539 JP0207539W WO03010302A1 WO 2003010302 A1 WO2003010302 A1 WO 2003010302A1 JP 0207539 W JP0207539 W JP 0207539W WO 03010302 A1 WO03010302 A1 WO 03010302A1
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WO
WIPO (PCT)
Prior art keywords
cultured
utilization
animal cells
construct containing
cell construct
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2002/007539
Other languages
English (en)
French (fr)
Inventor
Kazunori Kataoka
Hidenori Otsuka
Teruo Okano
Yukio Nagasaki
Yasuhiro Horiike
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Individual
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Application filed by Individual filed Critical Individual
Priority to CA2454807A priority Critical patent/CA2454807C/en
Priority to KR1020047000851A priority patent/KR100675533B1/ko
Priority to JP2003515653A priority patent/JP4111446B2/ja
Priority to US10/484,839 priority patent/US7470424B2/en
Priority to EP02755656A priority patent/EP1428871B1/en
Priority to CN028148428A priority patent/CN1798832B/zh
Priority to DE60228594T priority patent/DE60228594D1/de
Publication of WO2003010302A1 publication Critical patent/WO2003010302A1/ja
Anticipated expiration legal-status Critical
Priority to US12/230,916 priority patent/US7691369B2/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/28—Vascular endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/40—Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2535/00—Supports or coatings for cell culture characterised by topography
    • C12N2535/10—Patterned coating

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
PCT/JP2002/007539 2001-07-26 2002-07-25 Cultured cell construct containing spheroids of cultured animal cells and utilization thereof Ceased WO2003010302A1 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
CA2454807A CA2454807C (en) 2001-07-26 2002-07-25 Cultured cell construct which contains spheroids of cultured animal cells and the use thereof
KR1020047000851A KR100675533B1 (ko) 2001-07-26 2002-07-25 동물의 배양 세포의 스페로이드를 함유한 배양 세포구축물 및 그 사용
JP2003515653A JP4111446B2 (ja) 2001-07-26 2002-07-25 動物の培養細胞のスフェロイドを含む培養細胞構築物およびその使用
US10/484,839 US7470424B2 (en) 2001-07-26 2002-07-25 Cultured cell construct containing spheroids of cultured animal cells and utilization thereof
EP02755656A EP1428871B1 (en) 2001-07-26 2002-07-25 Cultured cell construct containing spheroids of cultured animal cells and utilization thereof
CN028148428A CN1798832B (zh) 2001-07-26 2002-07-25 含有动物培养细胞球状体的培养细胞构成物及其用途
DE60228594T DE60228594D1 (de) 2001-07-26 2002-07-25 Konstrukt aus kultivierten zellen mit spheroiden von kultivierten tierzellen und verwendung davon
US12/230,916 US7691369B2 (en) 2001-07-26 2008-09-08 Cultured cell construct which contains spheroids of cultured animal cells and the use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001-226293 2001-07-26
JP2001226293 2001-07-26

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10484839 A-371-Of-International 2002-07-25
US12/230,916 Division US7691369B2 (en) 2001-07-26 2008-09-08 Cultured cell construct which contains spheroids of cultured animal cells and the use thereof

Publications (1)

Publication Number Publication Date
WO2003010302A1 true WO2003010302A1 (en) 2003-02-06

Family

ID=19059133

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/007539 Ceased WO2003010302A1 (en) 2001-07-26 2002-07-25 Cultured cell construct containing spheroids of cultured animal cells and utilization thereof

Country Status (9)

Country Link
US (2) US7470424B2 (ja)
EP (2) EP1939280B1 (ja)
JP (1) JP4111446B2 (ja)
KR (1) KR100675533B1 (ja)
CN (1) CN1798832B (ja)
AT (1) ATE406436T1 (ja)
CA (1) CA2454807C (ja)
DE (2) DE60228594D1 (ja)
WO (1) WO2003010302A1 (ja)

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JP2007135593A (ja) * 2005-11-18 2007-06-07 Lifescan Inc 細胞クラスターの生成法
EP1961809A1 (en) 2007-02-20 2008-08-27 FUJIFILM Corporation Tissue construct-forming substrate, tissue construct-forming kit, method for forming tissue construct using the same and three-dimensional tissue construct formed by the method
JP2008545410A (ja) * 2005-05-24 2008-12-18 ザ レジェンツ オブ ザ ユニヴァースティ オブ カリフォルニア 微小規模のマイクロパターン化インビトロ操作・作製組織
WO2009050965A1 (ja) * 2007-10-19 2009-04-23 Japan As Represented By General Director Of Agency Of National Center For Child Health And Development 細胞凍結物固定化基材及び初代肝細胞培養ツール、並びに初代肝細胞培養ツールの製造方法
WO2009072590A1 (ja) * 2007-12-06 2009-06-11 Tokyo University Of Science Educational Foundation Administrative Organization 分岐ポリアルキレングリコール誘導体、感光性組成物、架橋体及び基板
WO2010047171A1 (ja) * 2008-10-22 2010-04-29 学校法人 東京女子医科大学 細胞パターン回収ツール
JP2010233538A (ja) * 2009-03-31 2010-10-21 Trans Parent:Kk 基質代謝物の評価方法
JP2013504303A (ja) * 2009-05-05 2013-02-07 ユニヴァシティー オブ ペーチュ 肺組織モデル
US8592139B2 (en) 2006-11-10 2013-11-26 Dai Nippon Printing Co., Ltd. Test method using cells and test kit therefor
US8741645B2 (en) 2006-11-10 2014-06-03 Dai Nippon Printing Co., Ltd. Test kit comprising a culture instrument with a cell pattern and a gel suitable to embed cell pattern
US8835173B2 (en) 2006-05-29 2014-09-16 Dai Nippon Printing Co., Ltd. Substrate for cell culture
JP2021525100A (ja) * 2018-05-16 2021-09-24 インセルム（インスティチュート ナショナル デ ラ サンテ エ デ ラ リシェルシェ メディカル） 増殖性肝細胞を培養する方法
KR20210119368A (ko) * 2019-12-05 2021-10-05 한국화학연구원 세포 비접착성 peg 고분자 기판 상에 형성된 심근세포 스페로이드 및 이의 제조방법

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EP1448053A4 (en) * 2001-10-02 2005-03-09 Becton Dickinson Co PROLIFERATION AND DIFFERENTIATION OF STEM CELLS BY EXTRACELLULAR MATRIX AND OTHER MOLECULES
US20060240400A1 (en) * 2003-02-06 2006-10-26 Masayuki Yamato High-density cell array board, process for producing the same and method of using the same
EP2267115B8 (en) * 2003-02-06 2014-07-23 Cellseed Inc. Anterior ocular segment related cell sheets, three-dimensional structures, and processes for producing the same
KR101141985B1 (ko) 2003-02-06 2012-05-17 고지 니시다 각막 상피 형성용 세포 시트, 그들의 제조 방법 및 그들의이용 방법
JP4649935B2 (ja) * 2004-02-02 2011-03-16 コニカミノルタホールディングス株式会社 インクジェットプリンタ
US8241905B2 (en) 2004-02-24 2012-08-14 The Curators Of The University Of Missouri Self-assembling cell aggregates and methods of making engineered tissue using the same
EP1857126B1 (en) * 2005-02-28 2018-10-24 CellSeed Inc. Cultured cell sheet, production method thereof, and application method thereof
WO2006093153A1 (ja) 2005-02-28 2006-09-08 Cellseed Inc. 培養細胞シート、製造方法及びその利用した組織修復方法
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US9127254B2 (en) 2006-12-18 2015-09-08 Ben-Gurion University Of The Negev Research And Development Authority Scaffolding for tissue regeneration or repair
EP3059302A1 (en) 2008-06-24 2016-08-24 The Curators Of The University Of Missouri Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same
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WO2010044417A1 (ja) * 2008-10-14 2010-04-22 株式会社セルシード 温度応答性細胞培養器材、及びその製造方法
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ATE406436T1 (de) 2008-09-15
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