DE60236974D1 - Kultiviertes Zellkonstrukt mit Sphäroiden kultivierter Tierzellen und seine Verwendung - Google Patents

Kultiviertes Zellkonstrukt mit Sphäroiden kultivierter Tierzellen und seine Verwendung

Info

Publication number
DE60236974D1
DE60236974D1 DE60236974T DE60236974T DE60236974D1 DE 60236974 D1 DE60236974 D1 DE 60236974D1 DE 60236974 T DE60236974 T DE 60236974T DE 60236974 T DE60236974 T DE 60236974T DE 60236974 D1 DE60236974 D1 DE 60236974D1
Authority
DE
Germany
Prior art keywords
spheroids
cultivated
animal cells
cell construct
cultured animal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60236974T
Other languages
English (en)
Inventor
Kazunori Kataoka
Hidenori Otsuka
Teruo Okano
Yukio Nagasaki
Yasuhiro Horiike
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Transparent Inc
Original Assignee
Transparent Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transparent Inc filed Critical Transparent Inc
Application granted granted Critical
Publication of DE60236974D1 publication Critical patent/DE60236974D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/28—Vascular endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/40—Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2535/00—Supports or coatings for cell culture characterised by topography
    • C12N2535/10—Patterned coating

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE60236974T 2001-07-26 2002-07-25 Kultiviertes Zellkonstrukt mit Sphäroiden kultivierter Tierzellen und seine Verwendung Expired - Lifetime DE60236974D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2001226293 2001-07-26

Publications (1)

Publication Number Publication Date
DE60236974D1 true DE60236974D1 (de) 2010-08-19

Family

ID=19059133

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60228594T Expired - Lifetime DE60228594D1 (de) 2001-07-26 2002-07-25 Konstrukt aus kultivierten zellen mit spheroiden von kultivierten tierzellen und verwendung davon
DE60236974T Expired - Lifetime DE60236974D1 (de) 2001-07-26 2002-07-25 Kultiviertes Zellkonstrukt mit Sphäroiden kultivierter Tierzellen und seine Verwendung

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE60228594T Expired - Lifetime DE60228594D1 (de) 2001-07-26 2002-07-25 Konstrukt aus kultivierten zellen mit spheroiden von kultivierten tierzellen und verwendung davon

Country Status (9)

Country Link
US (2) US7470424B2 (de)
EP (2) EP1428871B1 (de)
JP (1) JP4111446B2 (de)
KR (1) KR100675533B1 (de)
CN (1) CN1798832B (de)
AT (1) ATE406436T1 (de)
CA (1) CA2454807C (de)
DE (2) DE60228594D1 (de)
WO (1) WO2003010302A1 (de)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060270032A1 (en) * 2005-05-24 2006-11-30 The Regents Of The University Of California Microscale micropatterened engineered in vitro tissue
JP2005506839A (ja) * 2001-10-02 2005-03-10 ベクトン・ディキンソン・アンド・カンパニー 細胞外マトリックスおよび他の分子を使用する幹細胞の増殖および分化
WO2004069988A1 (ja) * 2003-02-06 2004-08-19 Cellseed Inc. 高密度細胞アレイ用基板、製造法、及びその利用方法
US8642338B2 (en) * 2003-02-06 2014-02-04 Cellseed Inc. Anterior ocular segment related cell sheets, three-dimensional structures, and processes for producing the same
KR101141985B1 (ko) 2003-02-06 2012-05-17 고지 니시다 각막 상피 형성용 세포 시트, 그들의 제조 방법 및 그들의이용 방법
JP4649935B2 (ja) * 2004-02-02 2011-03-16 コニカミノルタホールディングス株式会社 インクジェットプリンタ
US8241905B2 (en) 2004-02-24 2012-08-14 The Curators Of The University Of Missouri Self-assembling cell aggregates and methods of making engineered tissue using the same
US10533158B2 (en) * 2005-02-28 2020-01-14 Tokai University Educational System Cultured cell sheet, production method thereof, and application method thereof
JPWO2006093153A1 (ja) 2005-02-28 2008-08-07 株式会社セルシード 培養細胞シート、製造方法及びその利用した組織修復方法
AU2013202489B2 (en) * 2005-11-18 2016-03-03 Lifescan, Inc. A method for creating cell clusters
ES2605412T3 (es) * 2005-11-18 2017-03-14 Lifescan, Inc. Un método para crear grupos de células
JP5070565B2 (ja) 2006-05-29 2012-11-14 大日本印刷株式会社 細胞培養用基板
US20090203536A1 (en) * 2006-06-06 2009-08-13 Vermette Patrick Assay supports comprising a peg support, said support attached from a peg solution in cloud point (theta solvent) conditions
JP5261920B2 (ja) 2006-11-10 2013-08-14 大日本印刷株式会社 細胞を用いた試験法および試験用キット
US8592139B2 (en) 2006-11-10 2013-11-26 Dai Nippon Printing Co., Ltd. Test method using cells and test kit therefor
EP2120546B1 (de) * 2006-12-18 2016-09-28 Ben Gurion University Of The Negev Gerüst für die geweberegeneration oder reparatur
JP5583312B2 (ja) 2007-02-20 2014-09-03 富士フイルム株式会社 組織体形成用基材、組織体形成キット、それを用いた組織体形成法、及び該組織体形成法により形成された三次元組織体
US20100216245A1 (en) * 2007-10-19 2010-08-26 National Center For Child Health And Development Frozen cell immobilized product, primary hepatocyte culture tool, and method for producing primary hepatocyte culture tool
WO2009072590A1 (ja) * 2007-12-06 2009-06-11 Tokyo University Of Science Educational Foundation Administrative Organization 分岐ポリアルキレングリコール誘導体、感光性組成物、架橋体及び基板
WO2010008905A2 (en) 2008-06-24 2010-01-21 The Curators Of The University Of Missouri Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same
KR101005874B1 (ko) * 2008-08-27 2011-01-06 한길토건(주) 강재 재활용이 가능한 암파쇄 방호시설의 공법
US9598668B2 (en) * 2008-10-14 2017-03-21 Cellseed Inc. Temperature-responsive cell culture substrate and method for producing the same
JP5496488B2 (ja) * 2008-10-22 2014-05-21 学校法人東京女子医科大学 細胞パターン回収ツール
EP2358860A1 (de) * 2008-11-21 2011-08-24 Corning Incorporated Substrate und vorrichtungen mit beabstandeten vorsprüngen für die zellkultur
US20100166822A1 (en) * 2008-12-31 2010-07-01 Howmedica Osteonics Corp. Adhesive cartilage implant
JP2010233538A (ja) * 2009-03-31 2010-10-21 Trans Parent:Kk 基質代謝物の評価方法
JP2012523238A (ja) 2009-04-09 2012-10-04 ジ・アリゾナ・ボード・オブ・リージェンツ・オン・ビハーフ・オブ・ザ・ユニバーシティ・オブ・アリゾナ 三次元繊維芽細胞構築物の細胞播種および共培養
HUP0900819A2 (en) * 2009-05-05 2011-01-28 Pecsi Tudomanyegyetem Lung tissue culture
US8481303B2 (en) 2009-10-12 2013-07-09 Corning Incorporated Microfluidic device for cell culture
WO2011110722A1 (es) * 2010-03-12 2011-09-15 Fundación Progreso Y Salud Método para la proliferación "in vitro" de células procedentes de tejidos de origen endodérmico
CN101955595B (zh) * 2010-08-11 2012-01-04 东南大学 在多种材料表面制备化学微图案引导细胞定点生长的方法
WO2012048170A2 (en) * 2010-10-06 2012-04-12 Massachusetts Institute Of Technology Humanized animals via tissue engineering and uses therefor
EP2737051A1 (de) 2011-07-25 2014-06-04 Cytoo Verfahren und vorrichtung zur bildung dreidimensionaler multizellularanordnungen
CA2878404A1 (en) 2012-07-10 2014-01-16 The Trustees Of The University Of Pennsylvania Biomaterials for enhanced implant-host integration
EP2902496B1 (de) 2012-09-27 2018-04-18 Kuraray Co., Ltd. Verfahren zur bewertung des effekts von cytokin auf die stoffwechselaktivität von cytochrom p450 und verfahren zum screening von arzneimitteln
WO2014131019A2 (en) 2013-02-25 2014-08-28 Ohio State Innovation Foundation Her-1, her-3 and igf-1r compositions and uses thereof
WO2014143998A1 (en) * 2013-03-15 2014-09-18 The Broad Institute Systems and methods for culturing epithelial cells
US9790465B2 (en) 2013-04-30 2017-10-17 Corning Incorporated Spheroid cell culture well article and methods thereof
JP6122817B2 (ja) * 2014-03-26 2017-04-26 株式会社Ｓｃｒｅｅｎホールディングス スフェロイドの評価方法およびスフェロイド評価装置
KR101647793B1 (ko) 2014-09-02 2016-08-12 충남대학교산학협력단 온도 감응성 글리콜 키토산 유도체를 이용한 스페로이드 형성용 배양 용기 및 이를 이용한 스페로이드 형성 방법
KR102470508B1 (ko) 2014-10-29 2022-11-24 코닝 인코포레이티드 관류 생물반응기 플랫폼
CN107109328B (zh) 2014-10-29 2021-02-05 康宁股份有限公司 细胞培养插入件
CN104630148B (zh) * 2015-01-19 2017-06-06 西安交通大学 一种基于水凝胶微孔板的细胞球原位制备方法
CN104894051A (zh) * 2015-05-27 2015-09-09 成都易创思生物科技有限公司 一种新型的细胞培养方法
US11338065B2 (en) 2015-10-08 2022-05-24 Massachusetts Institute Of Technology In situ expansion of engineered devices for regeneration
KR20180104646A (ko) 2016-01-07 2018-09-21 비.지. 네게브 테크놀로지즈 앤드 애플리케이션스 리미티드, 엣 벤-구리온 유니버시티 면역관용 반응을 구축하기 위한 조성물 및 방법
KR102038496B1 (ko) * 2016-10-07 2019-10-30 울산과학기술원 다공성막을 이용한 현적 세포 배양 기기, 이의 제조 방법, 이를 이용한 현적 세포 배양 방법, 및 현적 세포 배양 자동화 장치
WO2019014635A1 (en) 2017-07-14 2019-01-17 Corning Incorporated 3D CELL CULTURE CONTAINERS FOR MANUAL OR AUTOMATIC EXCHANGE
EP3652291B1 (de) 2017-07-14 2021-12-29 Corning Incorporated Zellkulturgefäss
US11857970B2 (en) 2017-07-14 2024-01-02 Corning Incorporated Cell culture vessel
WO2019014610A1 (en) 2017-07-14 2019-01-17 Corning Incorporated CELL CULTURE CONTAINER FOR 3D CULTURE AND METHODS OF CULTURING 3D CELLS
WO2020013845A1 (en) 2018-07-13 2020-01-16 Corning Incorporated Cell culture vessels with stabilizer devices
JP7171696B2 (ja) 2018-07-13 2022-11-15 コーニング インコーポレイテッド 相互接続されたウェルを有するマイクロプレートを備えた流体デバイス
JP7171695B2 (ja) 2018-07-13 2022-11-15 コーニング インコーポレイテッド 液体培地送達面を含む側壁を有するマイクロキャビティ皿
KR20210070648A (ko) * 2019-12-05 2021-06-15 한국화학연구원 세포 비접착성 peg 고분자 기판 상에 형성된 심근세포 스페로이드 및 이의 제조방법

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2545100B1 (fr) * 1983-04-29 1985-12-20 Inst Nat Sante Rech Med Procede d'obtention de cultures d'hepatocytes humains, les cultures obtenues et leurs applications biologiques et biochimiques
US5037969A (en) 1986-07-03 1991-08-06 Takeda Chemical Industries, Ltd. Glycosyl derivatives and use thereof
JPH0751061B2 (ja) 1989-06-03 1995-06-05 鐘淵化学工業株式会社 細胞の配列制御用具の製法
DE3938632C1 (en) * 1989-11-21 1991-03-14 Adelbert Prof. Dr.Dr. 8046 Garching De Bacher Culturing living animal cells - using natural or synthetic polymer substrate vacuum deposition coated with e.g. nitride of Gp=III element
EP0529751A1 (de) * 1991-08-09 1993-03-03 W.R. Grace & Co.-Conn. Zellkulturträger und -testmaterial
US5776748A (en) 1993-10-04 1998-07-07 President And Fellows Of Harvard College Method of formation of microstamped patterns on plates for adhesion of cells and other biological materials, devices and uses therefor
JP2609073B2 (ja) 1994-10-26 1997-05-14 鐘淵化学工業株式会社 細胞の配列制御用具および細胞の配列制御法
AU5289096A (en) 1995-04-14 1996-10-30 Kazunori Kataoka Polyethylene oxides having saccharide residue at one end and different functional group at another end, and process for producing the same
CN1085987C (zh) 1995-04-19 2002-06-05 片冈一则 杂远螯嵌段共聚物及其制备方法
JP3711288B2 (ja) 1995-08-10 2005-11-02 一則 片岡 両末端に官能基を有するブロックポリマー
JP2002510969A (ja) * 1997-05-14 2002-04-09 ザ ジェネラル ホスピタル コーポレーション マイクロパターン化形態における細胞の共培養
JP3077628B2 (ja) 1997-05-27 2000-08-14 日本電気株式会社 細胞毒性試験方法
JP2973976B2 (ja) 1997-06-23 1999-11-08 日本電気株式会社 接着性細胞を用いた毒性試験方法

Also Published As

Publication number Publication date
EP1428871A1 (de) 2004-06-16
CN1798832B (zh) 2010-04-28
CN1798832A (zh) 2006-07-05
JPWO2003010302A1 (ja) 2005-06-02
EP1939280A1 (de) 2008-07-02
EP1428871A4 (de) 2004-11-24
WO2003010302A1 (fr) 2003-02-06
US20090011504A1 (en) 2009-01-08
KR100675533B1 (ko) 2007-01-29
EP1428871B1 (de) 2008-08-27
US7470424B2 (en) 2008-12-30
ATE406436T1 (de) 2008-09-15
JP4111446B2 (ja) 2008-07-02
KR20040025700A (ko) 2004-03-24
US7691369B2 (en) 2010-04-06
EP1939280B1 (de) 2010-07-07
DE60228594D1 (de) 2008-10-09
CA2454807C (en) 2012-09-18
CA2454807A1 (en) 2003-02-06
US20040197907A1 (en) 2004-10-07

Similar Documents

Publication Publication Date Title
DE60236974D1 (de) Kultiviertes Zellkonstrukt mit Sphäroiden kultivierter Tierzellen und seine Verwendung
DE50108487D1 (de) Bioreaktor für die kultivierung von mikroorganismen sowie verfahren zur herstellung desselben
ATE546517T1 (de) Neue, zur gattung paenibacillus gehörende stämme sowie verfahren zur bekämpfung von pflanzenkrankheiten unter verwendung dieser stämme oder einer kultur davon
WO2002059277A3 (en) Electroactive materials for stimulation of biological activity of stem cells
DE50105487D1 (de) Formkörper zur verbesserung der kultivierungsbedingungen von pflanzen
CO4820445A1 (es) Expresion de proteina en sistemas de expresion del vector baculovirus
DE60211119D1 (de) Kultivierung von crypthecodinium cohnii und abstammenden microorganismen
DK161785A (da) Fremgangsmaade til selektiv ukrudtsbekaempelse i nytteplantekulturer
DE60114865D1 (de) Heterologe herstellung von polyketiden
ATE274573T1 (de) Kulturgefäss
ATE466073T1 (de) Basalmedium zur kultivierung von es-zellen
DE69921297D1 (de) Pflanzenwachstumsmedium mit hydrophilem Membrane und Verwendung der Membrane zur Kontrolle der Feuchtigkeit
ATE272707T1 (de) Zellkulturgefäss für die kultivierung nicht adhärenter zellen
WO2004033616A8 (de) Einrichtung zur kultivierung von zellen, insbesondere menschlicher oder tierischer zellen
TR199801113T2 (xx) Rekombinan bakulovir�slerin �retimi.
DE60326003D1 (de) Ter verwendung des hepatozytenwachstumsfaktors
DE3872160D1 (de) Morpholinylsilane und verwendung zur beherrschung von pflanzenkrankheiten, verursacht durch pilze.
ES2191064T3 (es) Procedimiento para producir proteinas.
DE69534386D1 (de) Freigabe von intrazellulärem material
DE50001198D1 (de) Gewebekonstrukt für die Transplantationschirurgie
DE60023006D1 (de) Neues verfahren zur herstellung und auswahl von transgenen flachspflanzen und leinsamen
DE60319049D1 (de) Mikroorganismus zur Herstellung von Riboflavin, und seine Verwendung in der Herstellung von Riboflavin
FI910787A0 (fi) Foerfarande foer aseptisk rotning av in vitro foeroekat material avsett foer jordbruks- och traedgaordsbruk.
ATE423841T1 (de) Verwendung von glutaminfreien medium
DE60025036D1 (de) Verfahren zur züchtung von haftenden tierischen zellen