DE60236974D1 - Kultiviertes Zellkonstrukt mit Sphäroiden kultivierter Tierzellen und seine Verwendung - Google Patents

Kultiviertes Zellkonstrukt mit Sphäroiden kultivierter Tierzellen und seine Verwendung

Info

Publication number
DE60236974D1
DE60236974D1 DE60236974T DE60236974T DE60236974D1 DE 60236974 D1 DE60236974 D1 DE 60236974D1 DE 60236974 T DE60236974 T DE 60236974T DE 60236974 T DE60236974 T DE 60236974T DE 60236974 D1 DE60236974 D1 DE 60236974D1
Authority
DE
Germany
Prior art keywords
spheroids
cultivated
animal cells
cell construct
cultured animal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60236974T
Other languages
English (en)
Inventor
Kazunori Kataoka
Hidenori Otsuka
Teruo Okano
Yukio Nagasaki
Yasuhiro Horiike
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Transparent Inc
Original Assignee
Transparent Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transparent Inc filed Critical Transparent Inc
Application granted granted Critical
Publication of DE60236974D1 publication Critical patent/DE60236974D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/28—Vascular endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/40—Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2535/00—Supports or coatings for cell culture characterised by topography
    • C12N2535/10—Patterned coating

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE60236974T 2001-07-26 2002-07-25 Kultiviertes Zellkonstrukt mit Sphäroiden kultivierter Tierzellen und seine Verwendung Expired - Lifetime DE60236974D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2001226293 2001-07-26

Publications (1)

Publication Number Publication Date
DE60236974D1 true DE60236974D1 (de) 2010-08-19

Family

ID=19059133

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60228594T Expired - Lifetime DE60228594D1 (de) 2001-07-26 2002-07-25 Konstrukt aus kultivierten zellen mit spheroiden von kultivierten tierzellen und verwendung davon
DE60236974T Expired - Lifetime DE60236974D1 (de) 2001-07-26 2002-07-25 Kultiviertes Zellkonstrukt mit Sphäroiden kultivierter Tierzellen und seine Verwendung

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE60228594T Expired - Lifetime DE60228594D1 (de) 2001-07-26 2002-07-25 Konstrukt aus kultivierten zellen mit spheroiden von kultivierten tierzellen und verwendung davon

Country Status (9)

Country Link
US (2) US7470424B2 (de)
EP (2) EP1428871B1 (de)
JP (1) JP4111446B2 (de)
KR (1) KR100675533B1 (de)
CN (1) CN1798832B (de)
AT (1) ATE406436T1 (de)
CA (1) CA2454807C (de)
DE (2) DE60228594D1 (de)
WO (1) WO2003010302A1 (de)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005506839A (ja) * 2001-10-02 2005-03-10 ベクトン・ディキンソン・アンド・カンパニー 細胞外マトリックスおよび他の分子を使用する幹細胞の増殖および分化
EP2230298A1 (de) * 2003-02-06 2010-09-22 Cellseed Inc. Zellblatt des vorderen Augensegments, dreidimensionale Strukturen und Herstellungsverfahren dafür
KR101141985B1 (ko) * 2003-02-06 2012-05-17 고지 니시다 각막 상피 형성용 세포 시트, 그들의 제조 방법 및 그들의이용 방법
US20060240400A1 (en) * 2003-02-06 2006-10-26 Masayuki Yamato High-density cell array board, process for producing the same and method of using the same
JP4649935B2 (ja) * 2004-02-02 2011-03-16 コニカミノルタホールディングス株式会社 インクジェットプリンタ
WO2005081970A2 (en) 2004-02-24 2005-09-09 The Curators Of The University Of Missouri Self-assembling cell aggregates and methods of making engineered tissue using the same
WO2006093153A1 (ja) * 2005-02-28 2006-09-08 Cellseed Inc. 培養細胞シート、製造方法及びその利用した組織修復方法
ES2693609T3 (es) * 2005-02-28 2018-12-12 Cellseed Inc. Lámina de células cultivadas, método para su producción y método para su aplicación
JP2008545410A (ja) * 2005-05-24 2008-12-18 ザ レジェンツ オブ ザ ユニヴァースティ オブ カリフォルニア 微小規模のマイクロパターン化インビトロ操作・作製組織
JP5758061B2 (ja) * 2005-11-18 2015-08-05 ライフスキャン・インコーポレイテッドＬｉｆｅｓｃａｎ，Ｉｎｃ． 細胞クラスターの生成法
AU2013202489B2 (en) * 2005-11-18 2016-03-03 Lifescan, Inc. A method for creating cell clusters
JP5070565B2 (ja) 2006-05-29 2012-11-14 大日本印刷株式会社 細胞培養用基板
US20090203536A1 (en) * 2006-06-06 2009-08-13 Vermette Patrick Assay supports comprising a peg support, said support attached from a peg solution in cloud point (theta solvent) conditions
JP5261920B2 (ja) 2006-11-10 2013-08-14 大日本印刷株式会社 細胞を用いた試験法および試験用キット
US8592139B2 (en) 2006-11-10 2013-11-26 Dai Nippon Printing Co., Ltd. Test method using cells and test kit therefor
US9127254B2 (en) 2006-12-18 2015-09-08 Ben-Gurion University Of The Negev Research And Development Authority Scaffolding for tissue regeneration or repair
JP5583312B2 (ja) 2007-02-20 2014-09-03 富士フイルム株式会社 組織体形成用基材、組織体形成キット、それを用いた組織体形成法、及び該組織体形成法により形成された三次元組織体
WO2009050965A1 (ja) * 2007-10-19 2009-04-23 Japan As Represented By General Director Of Agency Of National Center For Child Health And Development 細胞凍結物固定化基材及び初代肝細胞培養ツール、並びに初代肝細胞培養ツールの製造方法
WO2009072590A1 (ja) * 2007-12-06 2009-06-11 Tokyo University Of Science Educational Foundation Administrative Organization 分岐ポリアルキレングリコール誘導体、感光性組成物、架橋体及び基板
KR20170020942A (ko) 2008-06-24 2017-02-24 더 큐레이터스 오브 더 유니버시티 오브 미주리 자가-조립가능 다세포체 및 이들을 이용하여 3차원 생물학적 구조체를 생산하는 방법
KR101005874B1 (ko) * 2008-08-27 2011-01-06 한길토건(주) 강재 재활용이 가능한 암파쇄 방호시설의 공법
EP2348099B1 (de) * 2008-10-14 2017-07-05 CellSeed Inc. Temperaturreaktives zellkultursubstrat und verfahren zu seiner herstellung
JP5496488B2 (ja) 2008-10-22 2014-05-21 学校法人東京女子医科大学 細胞パターン回収ツール
WO2010059583A1 (en) * 2008-11-21 2010-05-27 Corning Incorporated Spaced projection substrates and devices for cell culture
US20100166822A1 (en) * 2008-12-31 2010-07-01 Howmedica Osteonics Corp. Adhesive cartilage implant
JP2010233538A (ja) * 2009-03-31 2010-10-21 Trans Parent:Kk 基質代謝物の評価方法
CA2758208A1 (en) 2009-04-09 2010-10-14 Jordan Lancaster Cellular seeding and co-culture of a three dimensional fibroblast construct
HUP0900819A2 (en) * 2009-05-05 2011-01-28 Pecsi Tudomanyegyetem Lung tissue culture
US8481303B2 (en) 2009-10-12 2013-07-09 Corning Incorporated Microfluidic device for cell culture
EP2546334A4 (de) * 2010-03-12 2014-10-29 Fundación Progreso Y Salud Verfahren zur in-vitro-proliferation von aus gewebe endormalen ursprungs abgeleiteten zellen
CN101955595B (zh) * 2010-08-11 2012-01-04 东南大学 在多种材料表面制备化学微图案引导细胞定点生长的方法
WO2012048170A2 (en) * 2010-10-06 2012-04-12 Massachusetts Institute Of Technology Humanized animals via tissue engineering and uses therefor
CA2841902A1 (en) 2011-07-25 2013-01-31 Cytoo Methods and a device for the formation of three-dimensional multicellular assemblies
EP2872155A4 (de) 2012-07-10 2016-07-13 Univ Pennsylvania Biomaterialien für verbesserte implantat-host-integration
WO2014050139A1 (ja) 2012-09-27 2014-04-03 株式会社クラレ サイトカインがチトクロムｐ４５０の代謝能に及ぼす影響を評価する方法および薬剤のスクリーニング方法
WO2014131019A2 (en) 2013-02-25 2014-08-28 Ohio State Innovation Foundation Her-1, her-3 and igf-1r compositions and uses thereof
WO2014143998A1 (en) 2013-03-15 2014-09-18 The Broad Institute Systems and methods for culturing epithelial cells
US9790465B2 (en) 2013-04-30 2017-10-17 Corning Incorporated Spheroid cell culture well article and methods thereof
JP6122817B2 (ja) * 2014-03-26 2017-04-26 株式会社Ｓｃｒｅｅｎホールディングス スフェロイドの評価方法およびスフェロイド評価装置
KR101647793B1 (ko) * 2014-09-02 2016-08-12 충남대학교산학협력단 온도 감응성 글리콜 키토산 유도체를 이용한 스페로이드 형성용 배양 용기 및 이를 이용한 스페로이드 형성 방법
CN113481097A (zh) 2014-10-29 2021-10-08 康宁股份有限公司 灌注生物反应器平台
SG11201703493SA (en) 2014-10-29 2017-05-30 Corning Inc Cell culture insert
CN104630148B (zh) * 2015-01-19 2017-06-06 西安交通大学 一种基于水凝胶微孔板的细胞球原位制备方法
CN104894051A (zh) * 2015-05-27 2015-09-09 成都易创思生物科技有限公司 一种新型的细胞培养方法
WO2017062757A1 (en) 2015-10-08 2017-04-13 Massachusetts Institute Of Technology In situ expansion of engineered devices for regeneration
CN109069875B (zh) 2016-01-07 2021-12-24 位于本-古里安大学之内盖夫技术与应用有限公司 产生免疫耐受反应的组合物和方法
WO2018066953A1 (ko) * 2016-10-07 2018-04-12 울산과학기술원 다공성막을 이용한 현적 세포 배양 기기, 이의 제조 방법, 이를 이용한 현적 세포 배양 방법, 및 현적 세포 배양 자동화 장치
JP7245222B2 (ja) 2017-07-14 2023-03-23 コーニング インコーポレイテッド 手動又は自動で培地を交換するための３ｄ細胞培養容器
US11857970B2 (en) 2017-07-14 2024-01-02 Corning Incorporated Cell culture vessel
PL3652291T3 (pl) 2017-07-14 2022-03-28 Corning Incorporated Naczynie do hodowli komórkowej
EP3652292A1 (de) 2017-07-14 2020-05-20 Corning Incorporated Zellkulturgefäss für 3d-kultur und verfahren zur kultivierung von 3d-zellen
US11912968B2 (en) 2018-07-13 2024-02-27 Corning Incorporated Microcavity dishes with sidewall including liquid medium delivery surface
JP7171696B2 (ja) 2018-07-13 2022-11-15 コーニング インコーポレイテッド 相互接続されたウェルを有するマイクロプレートを備えた流体デバイス
US11732227B2 (en) 2018-07-13 2023-08-22 Corning Incorporated Cell culture vessels with stabilizer devices
KR20210070648A (ko) * 2019-12-05 2021-06-15 한국화학연구원 세포 비접착성 peg 고분자 기판 상에 형성된 심근세포 스페로이드 및 이의 제조방법

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2545100B1 (fr) * 1983-04-29 1985-12-20 Inst Nat Sante Rech Med Procede d'obtention de cultures d'hepatocytes humains, les cultures obtenues et leurs applications biologiques et biochimiques
US5037969A (en) 1986-07-03 1991-08-06 Takeda Chemical Industries, Ltd. Glycosyl derivatives and use thereof
JPH0751061B2 (ja) 1989-06-03 1995-06-05 鐘淵化学工業株式会社 細胞の配列制御用具の製法
DE3938632C1 (en) * 1989-11-21 1991-03-14 Adelbert Prof. Dr.Dr. 8046 Garching De Bacher Culturing living animal cells - using natural or synthetic polymer substrate vacuum deposition coated with e.g. nitride of Gp=III element
EP0529751A1 (de) * 1991-08-09 1993-03-03 W.R. Grace & Co.-Conn. Zellkulturträger und -testmaterial
US5776748A (en) 1993-10-04 1998-07-07 President And Fellows Of Harvard College Method of formation of microstamped patterns on plates for adhesion of cells and other biological materials, devices and uses therefor
JP2609073B2 (ja) 1994-10-26 1997-05-14 鐘淵化学工業株式会社 細胞の配列制御用具および細胞の配列制御法
SI9620064A (sl) 1995-04-14 1998-06-30 Kazunori Kataoka Polioksietilen s sladkorjem na enem koncu in različno funkcionalno skupino na drugem koncu ter postopek za njegovo pripravo
RU2169742C2 (ru) 1995-04-19 2001-06-27 Катаока Казунори Гетеротелехелатный блок-сополимер и способ его получения
AU726749B2 (en) 1995-08-10 2000-11-16 Kazunori Kataoka Block polymer having functional groups on its both ends
JP2002510969A (ja) * 1997-05-14 2002-04-09 ザ ジェネラル ホスピタル コーポレーション マイクロパターン化形態における細胞の共培養
JP3077628B2 (ja) * 1997-05-27 2000-08-14 日本電気株式会社 細胞毒性試験方法
JP2973976B2 (ja) 1997-06-23 1999-11-08 日本電気株式会社 接着性細胞を用いた毒性試験方法

Also Published As

Publication number Publication date
WO2003010302A1 (fr) 2003-02-06
US7470424B2 (en) 2008-12-30
US20090011504A1 (en) 2009-01-08
KR20040025700A (ko) 2004-03-24
CN1798832B (zh) 2010-04-28
CA2454807C (en) 2012-09-18
JPWO2003010302A1 (ja) 2005-06-02
US20040197907A1 (en) 2004-10-07
EP1939280A1 (de) 2008-07-02
CA2454807A1 (en) 2003-02-06
DE60228594D1 (de) 2008-10-09
ATE406436T1 (de) 2008-09-15
JP4111446B2 (ja) 2008-07-02
KR100675533B1 (ko) 2007-01-29
EP1428871A1 (de) 2004-06-16
US7691369B2 (en) 2010-04-06
EP1428871A4 (de) 2004-11-24
CN1798832A (zh) 2006-07-05
EP1939280B1 (de) 2010-07-07
EP1428871B1 (de) 2008-08-27

Similar Documents

Publication Publication Date Title
DE60236974D1 (de) Kultiviertes Zellkonstrukt mit Sphäroiden kultivierter Tierzellen und seine Verwendung
DE50108487D1 (de) Bioreaktor für die kultivierung von mikroorganismen sowie verfahren zur herstellung desselben
ATE546517T1 (de) Neue, zur gattung paenibacillus gehörende stämme sowie verfahren zur bekämpfung von pflanzenkrankheiten unter verwendung dieser stämme oder einer kultur davon
ATE289744T1 (de) Formkörper zur verbesserung der kultivierungsbedingungen von pflanzen
WO2002059277A3 (en) Electroactive materials for stimulation of biological activity of stem cells
CO4820445A1 (es) Expresion de proteina en sistemas de expresion del vector baculovirus
DE60211119D1 (de) Kultivierung von crypthecodinium cohnii und abstammenden microorganismen
DK161785A (da) Fremgangsmaade til selektiv ukrudtsbekaempelse i nytteplantekulturer
DE60114865D1 (de) Heterologe herstellung von polyketiden
ATE274573T1 (de) Kulturgefäss
ATE466073T1 (de) Basalmedium zur kultivierung von es-zellen
DE69921297D1 (de) Pflanzenwachstumsmedium mit hydrophilem Membrane und Verwendung der Membrane zur Kontrolle der Feuchtigkeit
DE50301710D1 (de) Kulturplate zum Bewurzeln von Jungpflanzen
DE50009938D1 (de) Verwendung von nanoskaligen wachsen
ATE272707T1 (de) Zellkulturgefäss für die kultivierung nicht adhärenter zellen
TR199801113T2 (xx) Rekombinan bakulovir�slerin �retimi.
DE60326003D1 (de) Ter verwendung des hepatozytenwachstumsfaktors
DE3872160D1 (de) Morpholinylsilane und verwendung zur beherrschung von pflanzenkrankheiten, verursacht durch pilze.
ES2191064T3 (es) Procedimiento para producir proteinas.
FR2801900B1 (fr) Methode de production de cellules animales adherentes
DE60023006D1 (de) Neues verfahren zur herstellung und auswahl von transgenen flachspflanzen und leinsamen
DE60319049D1 (de) Mikroorganismus zur Herstellung von Riboflavin, und seine Verwendung in der Herstellung von Riboflavin
FI910787A0 (fi) Foerfarande foer aseptisk rotning av in vitro foeroekat material avsett foer jordbruks- och traedgaordsbruk.
DE50311218D1 (de) Verwendung von glutaminfreien medium
ES2093182T3 (es) Antibiotico ab-041 y procedimiento para su obtencion.