DE60236974D1 - Cultivated cell construct with spheroids of cultured animal cells and its use - Google Patents

Cultivated cell construct with spheroids of cultured animal cells and its use

Info

Publication number
DE60236974D1
DE60236974D1 DE60236974T DE60236974T DE60236974D1 DE 60236974 D1 DE60236974 D1 DE 60236974D1 DE 60236974 T DE60236974 T DE 60236974T DE 60236974 T DE60236974 T DE 60236974T DE 60236974 D1 DE60236974 D1 DE 60236974D1
Authority
DE
Germany
Prior art keywords
spheroids
cultivated
animal cells
cell construct
cultured animal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60236974T
Other languages
German (de)
Inventor
Kazunori Kataoka
Hidenori Otsuka
Teruo Okano
Yukio Nagasaki
Yasuhiro Horiike
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Transparent Inc
Original Assignee
Transparent Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transparent Inc filed Critical Transparent Inc
Application granted granted Critical
Publication of DE60236974D1 publication Critical patent/DE60236974D1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/28—Vascular endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/40—Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2535/00—Supports or coatings for cell culture characterised by topography
    • C12N2535/10—Patterned coating

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Parenchymal cells are cultivated on cultivated endothelial cells or cultivated fibroblasts which have been separated by a surface of a specific hydrophilic polymer, and which have been patterned. A culture which contains thus formed patterned spheroids of cultivated parenchymal cells is thereby provided by this invention. This culture maintains a function which is specific to the parenchymal cells over a long period of time.
DE60236974T 2001-07-26 2002-07-25 Cultivated cell construct with spheroids of cultured animal cells and its use Expired - Lifetime DE60236974D1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2001226293 2001-07-26

Publications (1)

Publication Number Publication Date
DE60236974D1 true DE60236974D1 (en) 2010-08-19

Family

ID=19059133

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60228594T Expired - Lifetime DE60228594D1 (en) 2001-07-26 2002-07-25 CONSTRUCTION OF CULTIVATED CELLS WITH SPHEROIDS OF CULTIVATED ANIMAL CELLS AND USE THEREOF
DE60236974T Expired - Lifetime DE60236974D1 (en) 2001-07-26 2002-07-25 Cultivated cell construct with spheroids of cultured animal cells and its use

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE60228594T Expired - Lifetime DE60228594D1 (en) 2001-07-26 2002-07-25 CONSTRUCTION OF CULTIVATED CELLS WITH SPHEROIDS OF CULTIVATED ANIMAL CELLS AND USE THEREOF

Country Status (9)

Country Link
US (2) US7470424B2 (en)
EP (2) EP1939280B1 (en)
JP (1) JP4111446B2 (en)
KR (1) KR100675533B1 (en)
CN (1) CN1798832B (en)
AT (1) ATE406436T1 (en)
CA (1) CA2454807C (en)
DE (2) DE60228594D1 (en)
WO (1) WO2003010302A1 (en)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1448053A4 (en) * 2001-10-02 2005-03-09 Becton Dickinson Co Proliferation and differentiation of stem cells using extracellular matrix and other molecules
AU2003207288A1 (en) * 2003-02-06 2004-08-30 Cellseed Inc. Anterior ocular-associated cell sheet, three-dimensional construct and process for producing the same
WO2004069988A1 (en) * 2003-02-06 2004-08-19 Cellseed Inc. High-density cell array board, process for producing the same and method of using the same
KR101141985B1 (en) * 2003-02-06 2012-05-17 고지 니시다 Cell sheets for ectocornea formation, method of producing the same and method of using the same
JP4649935B2 (en) * 2004-02-02 2011-03-16 コニカミノルタホールディングス株式会社 Inkjet printer
US8241905B2 (en) 2004-02-24 2012-08-14 The Curators Of The University Of Missouri Self-assembling cell aggregates and methods of making engineered tissue using the same
EP1857126B1 (en) * 2005-02-28 2018-10-24 CellSeed Inc. Cultured cell sheet, production method thereof, and application method thereof
JPWO2006093153A1 (en) * 2005-02-28 2008-08-07 株式会社セルシード Cultured cell sheet, production method, and tissue repair method using the same
AU2006250079B2 (en) * 2005-05-24 2012-05-31 The Regents Of The University Of California Microscale micropatterned engineered in vitor tissue
AU2013202489B2 (en) * 2005-11-18 2016-03-03 Lifescan, Inc. A method for creating cell clusters
PL1788077T3 (en) * 2005-11-18 2017-03-31 Lifescan, Inc. A method for creating cell clusters
JP5070565B2 (en) 2006-05-29 2012-11-14 大日本印刷株式会社 Cell culture substrate
EP2064375B1 (en) * 2006-06-06 2012-11-21 Société de Commercialisation des Produits de la Recherche Appliquée - SOCPRA- Sciences et Génie s.e.c. Assay supports comprising a peg support, said support attached from a peg solution in cloud point (theta solvent) conditions
US8592139B2 (en) 2006-11-10 2013-11-26 Dai Nippon Printing Co., Ltd. Test method using cells and test kit therefor
JP5261920B2 (en) 2006-11-10 2013-08-14 大日本印刷株式会社 Test methods and test kits using cells
US9127254B2 (en) 2006-12-18 2015-09-08 Ben-Gurion University Of The Negev Research And Development Authority Scaffolding for tissue regeneration or repair
JP5583312B2 (en) 2007-02-20 2014-09-03 富士フイルム株式会社 Tissue formation substrate, tissue formation kit, tissue formation method using the same, and three-dimensional tissue formed by the tissue formation method
JPWO2009050965A1 (en) * 2007-10-19 2011-02-24 国立成育医療センター総長 Cell frozen material immobilization substrate, primary hepatocyte culture tool, and method for producing primary hepatocyte culture tool
WO2009072590A1 (en) * 2007-12-06 2009-06-11 Tokyo University Of Science Educational Foundation Administrative Organization Branched polyalkylene glycol derivative, photosensitive composition, crosslinked material, and substrate
KR20170020942A (en) 2008-06-24 2017-02-24 더 큐레이터스 오브 더 유니버시티 오브 미주리 Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same
KR101005874B1 (en) * 2008-08-27 2011-01-06 한길토건(주) Construction method of falling rock depense wall which is available for steel beam
EP2348099B1 (en) * 2008-10-14 2017-07-05 CellSeed Inc. Temperature-responsive cell culture substrate and method for producing the same
JP5496488B2 (en) * 2008-10-22 2014-05-21 学校法人東京女子医科大学 Cell pattern collection tool
EP2358860A1 (en) * 2008-11-21 2011-08-24 Corning Incorporated Spaced projection substrates and devices for cell culture
US20100166822A1 (en) * 2008-12-31 2010-07-01 Howmedica Osteonics Corp. Adhesive cartilage implant
JP2010233538A (en) * 2009-03-31 2010-10-21 Trans Parent:Kk Method for evaluating substrate metabolite
CA2758208A1 (en) 2009-04-09 2010-10-14 Jordan Lancaster Cellular seeding and co-culture of a three dimensional fibroblast construct
HUP0900819A2 (en) * 2009-05-05 2011-01-28 Pecsi Tudomanyegyetem Lung tissue culture
US8481303B2 (en) 2009-10-12 2013-07-09 Corning Incorporated Microfluidic device for cell culture
EP2546334A4 (en) * 2010-03-12 2014-10-29 Fundación Progreso Y Salud Method for the in vitro proliferation of cells derived from tissues of endormal origin
CN101955595B (en) * 2010-08-11 2012-01-04 东南大学 Method for guiding fixed-point cell growth by preparing chemical micro-patterns on surfaces of various materials
WO2012048170A2 (en) * 2010-10-06 2012-04-12 Massachusetts Institute Of Technology Humanized animals via tissue engineering and uses therefor
WO2013014164A1 (en) 2011-07-25 2013-01-31 Cytoo Methods and a device for the formation of three-dimensional multicellular assemblies
WO2014011775A1 (en) 2012-07-10 2014-01-16 The Trustees Of The University Of Pennsylvania Biomaterials for enhanced implant-host integration
WO2014050139A1 (en) 2012-09-27 2014-04-03 株式会社クラレ Method for evaluating influence of cytokine on metabolic capacity of cytochrome p450, and method for screening for medicinal agent
US10221230B2 (en) * 2013-02-25 2019-03-05 Ohio State Innovation Foundation HER-1, HER-3 and IGF-1R compositions and uses thereof
US10801015B2 (en) 2013-03-15 2020-10-13 The Broad Institute, Inc. Systems and methods for culturing epithelial cells
US9790465B2 (en) 2013-04-30 2017-10-17 Corning Incorporated Spheroid cell culture well article and methods thereof
JP6122817B2 (en) * 2014-03-26 2017-04-26 株式会社Ｓｃｒｅｅｎホールディングス Spheroid evaluation method and spheroid evaluation apparatus
KR101647793B1 (en) * 2014-09-02 2016-08-12 충남대학교산학협력단 Cell culture container for formation spheroid using thermosensitive glycol chitosan derivatives and spheroid formation method using the same
WO2016069895A1 (en) 2014-10-29 2016-05-06 Corning Incorporated Cell culture insert
CN113481097A (en) 2014-10-29 2021-10-08 康宁股份有限公司 Perfusion bioreactor platform
CN104630148B (en) * 2015-01-19 2017-06-06 西安交通大学 A kind of cell ball in-situ preparation method based on hydrogel microwell plate
CN104894051A (en) * 2015-05-27 2015-09-09 成都易创思生物科技有限公司 Novel cell culture method
US11338065B2 (en) 2015-10-08 2022-05-24 Massachusetts Institute Of Technology In situ expansion of engineered devices for regeneration
CN109069875B (en) 2016-01-07 2021-12-24 位于本-古里安大学之内盖夫技术与应用有限公司 Compositions and methods for generating immune tolerance responses
KR102038496B1 (en) * 2016-10-07 2019-10-30 울산과학기술원 Hanging drop cell culture device using porous membrane, method of manufacturing the same, method of hanging drop cell culturing, and automatic hanging drop cell culture device
CN111065729B (en) 2017-07-14 2024-04-26 康宁股份有限公司 Cell culture vessel
JP7245222B2 (en) 2017-07-14 2023-03-23 コーニング インコーポレイテッド 3D cell culture vessel for manual or automatic medium exchange
US11857970B2 (en) 2017-07-14 2024-01-02 Corning Incorporated Cell culture vessel
EP3652292A1 (en) 2017-07-14 2020-05-20 Corning Incorporated Cell culture vessel for 3d culture and methods of culturing 3d cells
JP2020534018A (en) * 2017-09-22 2020-11-26 ライフネット ヘルス Seeded hepatocytes and their preparation and use
JP7171695B2 (en) 2018-07-13 2022-11-15 コーニング インコーポレイテッド A microcavity dish having sidewalls containing a liquid medium delivery surface
JP7353263B2 (en) 2018-07-13 2023-09-29 コーニング インコーポレイテッド Cell culture vessel with stabilization device
US11661574B2 (en) 2018-07-13 2023-05-30 Corning Incorporated Fluidic devices including microplates with interconnected wells
KR20210070648A (en) * 2019-12-05 2021-06-15 한국화학연구원 cardiomyocyte spheroid formed on cell nonadhesive PEG polymer substrate and method for preparing the same

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2545100B1 (en) * 1983-04-29 1985-12-20 Inst Nat Sante Rech Med PROCESS FOR OBTAINING CULTURES OF HUMAN HEPATOCYTES, THE CULTURES OBTAINED AND THEIR BIOLOGICAL AND BIOCHEMICAL APPLICATIONS
US5037969A (en) 1986-07-03 1991-08-06 Takeda Chemical Industries, Ltd. Glycosyl derivatives and use thereof
JPH0751061B2 (en) 1989-06-03 1995-06-05 鐘淵化学工業株式会社 Manufacturing method of cell array control tool
DE3938632C1 (en) * 1989-11-21 1991-03-14 Adelbert Prof. Dr.Dr. 8046 Garching De Bacher Culturing living animal cells - using natural or synthetic polymer substrate vacuum deposition coated with e.g. nitride of Gp=III element
EP0529751A1 (en) * 1991-08-09 1993-03-03 W.R. Grace & Co.-Conn. Cell culture substrate, test material for cell culture and preparations thereof
US5776748A (en) * 1993-10-04 1998-07-07 President And Fellows Of Harvard College Method of formation of microstamped patterns on plates for adhesion of cells and other biological materials, devices and uses therefor
JP2609073B2 (en) 1994-10-26 1997-05-14 鐘淵化学工業株式会社 Cell arrangement control tool and cell arrangement control method
DK0852243T3 (en) 1995-04-14 2003-02-24 Kazunori Kataoka Polyethylene oxides having a saccharide group at one end and another functional group at the other end, and a process for their preparation
EP0822217B1 (en) 1995-04-19 2002-10-23 Kazunori Kataoka Heterotelechelic block copolymers and process for producing the same
SI9620107A (en) 1995-08-10 1998-10-31 Kazunori Kataoka Block polymer having functional groups at both ends
JP2002510969A (en) * 1997-05-14 2002-04-09 ザ ジェネラル ホスピタル コーポレーション Co-culture of cells in micropatterned morphology
JP3077628B2 (en) * 1997-05-27 2000-08-14 日本電気株式会社 Cytotoxicity test method
JP2973976B2 (en) 1997-06-23 1999-11-08 日本電気株式会社 Toxicity test method using adherent cells

Also Published As

Publication number Publication date
CA2454807A1 (en) 2003-02-06
KR20040025700A (en) 2004-03-24
US7470424B2 (en) 2008-12-30
JP4111446B2 (en) 2008-07-02
CN1798832A (en) 2006-07-05
US20040197907A1 (en) 2004-10-07
KR100675533B1 (en) 2007-01-29
EP1428871A1 (en) 2004-06-16
EP1428871B1 (en) 2008-08-27
US20090011504A1 (en) 2009-01-08
JPWO2003010302A1 (en) 2005-06-02
CN1798832B (en) 2010-04-28
DE60228594D1 (en) 2008-10-09
EP1939280A1 (en) 2008-07-02
WO2003010302A1 (en) 2003-02-06
CA2454807C (en) 2012-09-18
US7691369B2 (en) 2010-04-06
EP1428871A4 (en) 2004-11-24
ATE406436T1 (en) 2008-09-15
EP1939280B1 (en) 2010-07-07

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