RU2011124900A - Культура плюрипотентных стволовых клеток на микроносителях - Google Patents

Культура плюрипотентных стволовых клеток на микроносителях Download PDF

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RU2011124900A
RU2011124900A RU2011124900/10A RU2011124900A RU2011124900A RU 2011124900 A RU2011124900 A RU 2011124900A RU 2011124900/10 A RU2011124900/10 A RU 2011124900/10A RU 2011124900 A RU2011124900 A RU 2011124900A RU 2011124900 A RU2011124900 A RU 2011124900A
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Шелли НЕЛЬСОН
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Сентокор Орто Байотек Инк.
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Abstract

1. Способ выращивания плюрипотентных стволовых клеток, включающий следующие этапы:а. прикрепление популяции плюрипотентных стволовых клеток к первому объему микроносителей;b. культивирование плюрипотентных стволовых клеток на первом объеме микроносителей;c. отделение плюрипотентных стволовых клеток от первого объема микроносителей;d. прикрепление популяции плюрипотентных стволовых клеток ко второму объему микроносителей.2. Способ по п.1, в котором этапы культивирования, отделения и прикрепления плюрипотентных стволовых клеток на микроносителях повторяются с использованием следующих объемов микроносителей.3. Способ по п.1, в котором первый объем микроносителей выбирается из группы, состоящей из декстрановых микроносителей и полистироловых микроносителей.4. Способ по п.1, в котором второй объем микроносителей выбирается из группы, состоящей из декстрановых микроносителей и полистироловых микроносителей.5. Способ по п.1, в котором плюрипотентные стволовые клетки прикрепляются к первому объему микроносителей в среде, содержащей ингибитор Rho-киназы.6. Способ по п.1, в котором плюрипотентные стволовые клетки прикрепляются ко второму объему микроносителей в среде, содержащей ингибитор Rho-киназы.7. Способ по п.1, в котором плюрипотентные стволовые клетки отделяются от первого объема микроносителей путем ферментативной обработки.8. Способ по п.1, в котором плюрипотентные стволовые клетки отделяются от второго объема микроносителей путем ферментативной обработки.9. Способ по п.1, в котором первый объем микроносителей отделяется от плюрипотентных стволовых клеток перед прикреплением клеток ко второму объему микро

Claims (10)

1. Способ выращивания плюрипотентных стволовых клеток, включающий следующие этапы:
а. прикрепление популяции плюрипотентных стволовых клеток к первому объему микроносителей;
b. культивирование плюрипотентных стволовых клеток на первом объеме микроносителей;
c. отделение плюрипотентных стволовых клеток от первого объема микроносителей;
d. прикрепление популяции плюрипотентных стволовых клеток ко второму объему микроносителей.
2. Способ по п.1, в котором этапы культивирования, отделения и прикрепления плюрипотентных стволовых клеток на микроносителях повторяются с использованием следующих объемов микроносителей.
3. Способ по п.1, в котором первый объем микроносителей выбирается из группы, состоящей из декстрановых микроносителей и полистироловых микроносителей.
4. Способ по п.1, в котором второй объем микроносителей выбирается из группы, состоящей из декстрановых микроносителей и полистироловых микроносителей.
5. Способ по п.1, в котором плюрипотентные стволовые клетки прикрепляются к первому объему микроносителей в среде, содержащей ингибитор Rho-киназы.
6. Способ по п.1, в котором плюрипотентные стволовые клетки прикрепляются ко второму объему микроносителей в среде, содержащей ингибитор Rho-киназы.
7. Способ по п.1, в котором плюрипотентные стволовые клетки отделяются от первого объема микроносителей путем ферментативной обработки.
8. Способ по п.1, в котором плюрипотентные стволовые клетки отделяются от второго объема микроносителей путем ферментативной обработки.
9. Способ по п.1, в котором первый объем микроносителей отделяется от плюрипотентных стволовых клеток перед прикреплением клеток ко второму объему микроносителей.
10. Способ дифференцирования плюрипотентных стволовых клеток в инсулинэкспрессирующие клетки, состоящий из следующих этапов:
а. прикрепление плюрипотентных стволовых клеток к объему микроносителей;
b. дифференцирование плюрипотентных стволовых клеток в клетки, экспрессирующие маркеры, характерные для линии сформированной эндодермы;
c. дифференцирование клеток, экспрессирующих маркеры, характерные для линии сформированной эндодермы, в клетки, экспрессирующие маркеры, характерные для линии панкреатической эндодермы;
d. дифференцирование клеток, экспрессирующих маркеры, характерные для линии панкреатической эндодермы, в клетки, экспрессирующие маркеры, характерные для линии панкреатических эндокринных клеток;
e. дифференцирование клеток, экспрессирующих маркеры, характерные для линии панкреатических эндокринных клеток, в инсулинэкспрессирующие клетки.
RU2011124900/10A 2008-11-20 2009-11-19 Культура плюрипотентных стволовых клеток на микроносителях RU2555538C2 (ru)

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US11644708P 2008-11-20 2008-11-20
US61/116,447 2008-11-20
PCT/US2009/065062 WO2010059775A1 (en) 2008-11-20 2009-11-19 Pluripotent stem cell culture on micro-carriers

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Families Citing this family (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100381768B1 (ko) * 2001-08-21 2003-04-26 김승환 무선 에이티엠망의 동적 자원할당 매체접속제어 프로토콜
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
RU2473685C2 (ru) 2007-07-31 2013-01-27 Лайфскен, Инк. Дифференцировка человеческих эмбриональных стволовых клеток
CN101878298B (zh) 2007-11-27 2017-08-15 生命扫描有限公司 人胚胎干细胞的分化
CN105886459A (zh) 2008-02-21 2016-08-24 詹森生物科技公司 用于细胞粘附、培养和分离的方法、表面改性培养板和组合物
US9458431B2 (en) 2008-03-17 2016-10-04 Agency For Science, Technology And Research Microcarriers for stem cell culture
US20110143433A1 (en) * 2008-03-17 2011-06-16 Agency For Science, Technology And Research Microcarriers for Stem Cell Culture
US8828720B2 (en) 2008-03-17 2014-09-09 Agency For Science, Technology And Research Microcarriers for stem cell culture
US8691569B2 (en) 2008-03-17 2014-04-08 Agency For Science, Technology And Research Microcarriers for stem cell culture
JP2011514169A (ja) 2008-03-17 2011-05-06 エージェンシー フォー サイエンス，テクノロジー アンド リサーチ 幹細胞培養のためのマイクロキャリア
KR101829310B1 (ko) 2008-06-30 2018-02-14 얀센 바이오테크 인코포레이티드 만능 줄기 세포의 분화
ES2634445T3 (es) 2008-10-31 2017-09-27 Janssen Biotech, Inc. Diferenciación de las células madre embrionarias humanas con el linaje endocrino pancreático
EP2350265B1 (en) 2008-10-31 2019-04-17 Janssen Biotech, Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
US20100124781A1 (en) 2008-11-20 2010-05-20 Shelley Nelson Pluripotent Stem Cell Culture on Micro-Carriers
JP5719305B2 (ja) 2008-11-20 2015-05-13 ヤンセン バイオテツク，インコーポレーテツド 平面支持体上での細胞付着及び培養のための方法及び組成物
EP2408903B1 (en) 2009-03-20 2014-08-06 Agency For Science, Technology And Research Culture of pluripotent and multipotent cells on microcarriers
RU2579278C2 (ru) 2009-07-20 2016-04-10 Янссен Байотек, Инк. Популяция панкреатических эндокринных клеток-предшественников для снижения концентрации глюкозы в крови и способ дифференцировки панкреатических эндодермальных клеток
CA2784415C (en) 2009-12-23 2019-06-18 Jean Xu Differentiation of human embryonic stem cells
CN102791851B (zh) 2010-03-01 2017-07-14 詹森生物科技公司 纯化衍生自多能干细胞的细胞的方法
EP3498825A1 (en) 2010-05-12 2019-06-19 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
EP3228629A1 (en) * 2010-06-16 2017-10-11 Minerva Biotechnologies Corporation Reprogramming cancer cells
SG10201506855RA (en) 2010-08-31 2015-10-29 Janssen Biotech Inc Differentiation of human embryonic stem cells
BR112013004514A2 (pt) * 2010-08-31 2016-06-07 Janssen Biotech Inc diferenciação das células-tronco embrionárias humanas
CA2809305C (en) 2010-08-31 2019-06-11 Janssen Biotech, Inc. Differentiation of pluripotent stem cells
CN105112361B (zh) * 2010-09-02 2018-11-16 生命技术公司 用于细胞的生物反应器放大的细胞培养系统
EP2622065B1 (en) 2010-10-01 2016-09-07 The Government of the United States of America as represented by the Secretary of the Department of Health and Human Services Manipulation of stem cell function by p53 isoforms
JP5938726B2 (ja) * 2010-10-28 2016-06-22 国立大学法人 熊本大学 多能性幹細胞の分化誘導効率を改善するための方法及び培地
DK2638149T3 (da) 2010-11-12 2019-08-12 Univ Georgetown Immortalisering af epitelceller og fremgangsmåder til anvendelse
US9453196B2 (en) 2010-12-16 2016-09-27 General Electric Company Cell carrier, methods of making and use
US9534206B2 (en) 2010-12-16 2017-01-03 General Electric Company Cell carrier, associated methods for making cell carrier and culturing cells using the same
US9453197B2 (en) 2010-12-16 2016-09-27 General Electric Company Methods of making cell carrier
US9926523B2 (en) 2010-12-16 2018-03-27 General Electric Company Cell carriers and methods for culturing cells
US9518249B2 (en) 2010-12-16 2016-12-13 General Electric Company Cell carrier, associated methods for making cell carrier and culturing cells using the same
RU2705001C2 (ru) 2011-12-22 2019-11-01 Янссен Байотек, Инк. Дифференцировка эмбриональных стволовых клеток человека в одногормональные инсулинположительные клетки
EP2823036B1 (en) * 2012-03-07 2019-04-17 F.Hoffmann-La Roche Ag Method of determining teratogenic risk
EP2823037A4 (en) 2012-03-07 2015-09-16 Janssen Biotech Inc DEFINED MEDIA FOR THE EXPANSION AND CARE OF PLURIPOTENTAL STEM CELLS
AU2013271581B2 (en) 2012-06-08 2018-08-09 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endocrine cells
DK2878664T3 (en) 2012-07-24 2018-11-05 Nissan Chemical Corp CULTURE MEDIUM COMPOSITION AND PROCEDURE FOR CULTIVATING CELLS OR TISSUE USING THE COMPOSITION
US9664671B2 (en) 2012-07-24 2017-05-30 Nissan Chemical Industries, Ltd. Culture medium composition and method of culturing cell or tissue using thereof
US10017805B2 (en) 2012-08-23 2018-07-10 Nissan Chemical Industries, Ltd. Enhancing ingredients for protein production from various cells
EP2893000B1 (en) 2012-09-03 2019-04-10 Novo Nordisk A/S Generation of pancreatic endoderm from pluripotent stem cells using small molecules
CA2896750A1 (en) * 2012-12-31 2014-07-03 Janssen Biotech, Inc. Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells
BR112015015701A2 (pt) 2012-12-31 2017-07-11 Janssen Biotech Inc diferenciação de células-tronco embrionárias humanas em células pancreáticas endócrinas com o uso de reguladores hb9
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
WO2014105543A1 (en) 2012-12-31 2014-07-03 Janssen Biotech, Inc. Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
WO2014106947A1 (en) * 2013-01-04 2014-07-10 Kyoto University Reprogrammed stem cell
AU2014218807A1 (en) * 2013-02-22 2015-09-03 Cellular Dynamics International, Inc. Hepatocyte production via forward programming by combined genetic and chemical engineering
US10004767B2 (en) * 2013-09-19 2018-06-26 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for producing hepatocyte-like cells
KR102282265B1 (ko) 2014-01-23 2021-07-26 닛산 가가쿠 가부시키가이샤 배양 배지 조성물의 제조 방법
TWI675101B (zh) 2014-01-23 2019-10-21 日商日產化學工業股份有限公司 培養基組成物
DK3143127T3 (da) 2014-05-16 2021-09-13 Janssen Biotech Inc Anvendelse af små molekyler til at forstærke mafa-ekspression i endokrine pankreasceller
CN104450604A (zh) * 2014-11-25 2015-03-25 成都威尔诺生物科技有限公司 用于制备疫苗工艺的微载体的清洁、灭菌的方法
US9944894B2 (en) 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
DK3368657T3 (da) 2015-10-30 2021-10-04 Biolamina Ab Fremgangsmåder til fremstilling af hepatocytter
JPWO2017170849A1 (ja) * 2016-03-30 2019-05-16 協和発酵バイオ株式会社 ナイーブ型多能性幹細胞培養用培地および多能性幹細胞の培養方法
MA45479A (fr) 2016-04-14 2019-02-20 Janssen Biotech Inc Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen
US20200306299A9 (en) * 2016-10-21 2020-10-01 Georgia Tech Research Corporation Methods and Systems for T Cell Expansion
WO2018213721A1 (en) * 2017-05-19 2018-11-22 Thrive Bioscience, Inc. Systems and methods for cell dissociation
WO2019102593A1 (ja) * 2017-11-24 2019-05-31 株式会社Ihi 細胞培養装置
CN108753681B (zh) * 2018-04-28 2022-02-15 中山大学附属第一医院 一种鼻上皮干细胞培养方法及鼻上皮干细胞增殖培养基
KR102115360B1 (ko) * 2018-05-30 2020-05-26 주식회사 바이블리오테카 성체 줄기세포 배양액 및 그 제조방법
CN110564675A (zh) * 2019-09-30 2019-12-13 广东华夏健康生命科学有限公司 一种毛囊干细胞的分离提取方法
CN111040983A (zh) * 2019-12-25 2020-04-21 杭州原生生物科技有限公司 一种3d微载体细胞吸附培养的方法
CN111424011A (zh) * 2020-04-17 2020-07-17 深圳市旷逸生物科技有限公司 一种能够维持脐带间充质干细胞细胞形态的三维培养方法
CN111206015B (zh) * 2020-04-21 2020-07-31 广东省生物资源应用研究所 一种利用fact ⅲ微载体体外扩增精原干细胞的三维动态培养方法
CN113637635B (zh) * 2021-10-13 2022-01-25 北京华龛生物科技有限公司 微载体细胞培养体系中微载体聚团状态的解散方法
CN115089614A (zh) * 2022-06-28 2022-09-23 中国人民解放军军事科学院军事医学研究院 一种增强骨骼干细胞性能的方法及其在治疗骨关节炎中的应用

Family Cites Families (250)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US264713A (en) * 1882-09-19 Neck-strap buckle and rein-ring
US3209652A (en) 1961-03-30 1965-10-05 Burgsmueller Karl Thread whirling method
AT326803B (de) 1968-08-26 1975-12-29 Binder Fa G Maschenware sowie verfahren zur herstellung derselben
US3935067A (en) 1974-11-22 1976-01-27 Wyo-Ben Products, Inc. Inorganic support for culture media
CA1201400A (en) 1982-04-16 1986-03-04 Joel L. Williams Chemically specific surfaces for influencing cell activity during culture
US4499802A (en) 1982-09-29 1985-02-19 Container Graphics Corporation Rotary cutting die with scrap ejection
US4537773A (en) 1983-12-05 1985-08-27 E. I. Du Pont De Nemours And Company α-Aminoboronic acid derivatives
US4557264A (en) 1984-04-09 1985-12-10 Ethicon Inc. Surgical filament from polypropylene blended with polyethylene
US5089396A (en) 1985-10-03 1992-02-18 Genentech, Inc. Nucleic acid encoding β chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid
US5215893A (en) 1985-10-03 1993-06-01 Genentech, Inc. Nucleic acid encoding the ba chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid
US4737578A (en) 1986-02-10 1988-04-12 The Salk Institute For Biological Studies Human inhibin
US5863531A (en) 1986-04-18 1999-01-26 Advanced Tissue Sciences, Inc. In vitro preparation of tubular tissue structures by stromal cell culture on a three-dimensional framework
US5567612A (en) 1986-11-20 1996-10-22 Massachusetts Institute Of Technology Genitourinary cell-matrix structure for implantation into a human and a method of making
US5804178A (en) 1986-11-20 1998-09-08 Massachusetts Institute Of Technology Implantation of cell-matrix structure adjacent mesentery, omentum or peritoneum tissue
CA1340581C (en) 1986-11-20 1999-06-08 Joseph P. Vacanti Chimeric neomorphogenesis of organs by controlled cellular implantation using artificial matrices
NZ229354A (en) 1988-07-01 1990-09-26 Becton Dickinson Co Treating polymer surfaces with a gas plasma and then applying a layer of endothelial cells to the surface
EP0363125A3 (en) 1988-10-03 1990-08-16 Hana Biologics Inc. Proliferated pancreatic endocrine cell product and process
US5837539A (en) 1990-11-16 1998-11-17 Osiris Therapeutics, Inc. Monoclonal antibodies for human mesenchymal stem cells
AU668349B2 (en) 1991-04-25 1996-05-02 Chugai Seiyaku Kabushiki Kaisha Reconstituted human antibody against human interleukin 6 receptor
US5449383A (en) 1992-03-18 1995-09-12 Chatelier; Ronald C. Cell growth substrates
GB9206861D0 (en) 1992-03-28 1992-05-13 Univ Manchester Wound healing and treatment of fibrotic disorders
CA2114282A1 (en) 1993-01-28 1994-07-29 Lothar Schilder Multi-layered implant
JP3525221B2 (ja) 1993-02-17 2004-05-10 味の素株式会社 免疫抑制剤
AU687386B2 (en) 1993-04-08 1998-02-26 Human Cell Cultures, Inc. Cell culturing method and medium
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
GB9310557D0 (en) 1993-05-21 1993-07-07 Smithkline Beecham Plc Novel process and apparatus
TW257671B (ru) 1993-11-19 1995-09-21 Ciba Geigy
US6703017B1 (en) 1994-04-28 2004-03-09 Ixion Biotechnology, Inc. Reversal of insulin-dependent diabetes by islet-producing stem cells, islet progenitor cells and islet-like structures
US5834308A (en) 1994-04-28 1998-11-10 University Of Florida Research Foundation, Inc. In vitro growth of functional islets of Langerhans
US6001647A (en) 1994-04-28 1999-12-14 Ixion Biotechnology, Inc. In vitro growth of functional islets of Langerhans and in vivo uses thereof
US6083903A (en) 1994-10-28 2000-07-04 Leukosite, Inc. Boronic ester and acid compounds, synthesis and uses
ES2170815T5 (es) 1994-12-29 2012-11-14 Chugai Seiyaku Kabushiki Kaisha Uso de un anticuerpo PM-1 o de un anticuerpo MH166 para potenciar el efecto antitumoral de cisplatino o carboplatino
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5718922A (en) 1995-05-31 1998-02-17 Schepens Eye Research Institute, Inc. Intravitreal microsphere drug delivery and method of preparation
US5908782A (en) 1995-06-05 1999-06-01 Osiris Therapeutics, Inc. Chemically defined medium for human mesenchymal stem cells
US5681561A (en) 1995-06-07 1997-10-28 Life Medical Sciences, Inc. Compositions and methods for improving autologous fat grafting
ATE244234T1 (de) 1997-04-24 2003-07-15 Ortho Mcneil Pharm Inc Substituierte imidazole zur behandlung von entzündlichen krankheiten
EP1028737B1 (en) 1997-07-03 2007-04-04 Osiris Therapeutics, Inc. Human mesenchymal stem cells from peripheral blood
DK1538206T3 (da) 1997-09-16 2010-07-12 Centocor Ortho Biotech Inc Fremgangsmåde til fuldstændig kemisk syntese og samling af gener og genomer
US6670127B2 (en) 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
JP3880795B2 (ja) 1997-10-23 2007-02-14 ジェロン・コーポレーション フィーダー細胞を含まない培養物中で、霊長類由来始原幹細胞を増殖させるための方法
US6372779B1 (en) 1997-12-29 2002-04-16 Ortho Pharmaceutical Corporation Anti-inflammatory compounds
ES2258329T3 (es) 1998-03-18 2006-08-16 Osiris Therapeutics, Inc. Celulas madre mesenquimaticas para la prevencion y tratamiento de respuestas inmunes en trasplantes.
MY132496A (en) 1998-05-11 2007-10-31 Vertex Pharma Inhibitors of p38
US6413773B1 (en) 1998-06-01 2002-07-02 The Regents Of The University Of California Phosphatidylinositol 3-kinase inhibitors as stimulators of endocrine differentiation
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US6610540B1 (en) 1998-11-18 2003-08-26 California Institute Of Technology Low oxygen culturing of central nervous system progenitor cells
US6413556B1 (en) 1999-01-08 2002-07-02 Sky High, Llc Aqueous anti-apoptotic compositions
IL144359A0 (en) 1999-01-21 2002-05-23 Vitro Diagnostics Inc Immortalized cell lines and methods of making the same
US6815203B1 (en) 1999-06-23 2004-11-09 Joslin Diabetes Center, Inc. Methods of making pancreatic islet cells
US6333029B1 (en) 1999-06-30 2001-12-25 Ethicon, Inc. Porous tissue scaffoldings for the repair of regeneration of tissue
US6306424B1 (en) 1999-06-30 2001-10-23 Ethicon, Inc. Foam composite for the repair or regeneration of tissue
CA2385628A1 (en) 1999-09-27 2001-04-05 Ammon B. Peck Reversal of insulin-dependent diabetes by islet-producing stem cells, islet progenitor cells and islet-like structures
US6685936B2 (en) 1999-10-12 2004-02-03 Osiris Therapeutics, Inc. Suppressor cells induced by culture with mesenchymal stem cells for treatment of immune responses in transplantation
US20030082155A1 (en) 1999-12-06 2003-05-01 Habener Joel F. Stem cells of the islets of langerhans and their use in treating diabetes mellitus
EP1240518A4 (en) 1999-12-13 2006-05-17 Scripps Research Inst MARKERS FOR THE IDENTIFICATION AND INSULATION OF PRE-GENERIC CELLS OF A AND B PANCREAS ISOLATED CELLS
US7439064B2 (en) 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
US7005252B1 (en) 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
US6436704B1 (en) 2000-04-10 2002-08-20 Raven Biotechnologies, Inc. Human pancreatic epithelial progenitor cells and methods of isolation and use thereof
US6458589B1 (en) 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
AU2001274624A1 (en) 2000-06-26 2002-01-08 Renomedix Institute Inc. Cell fraction containing cells capable of differentiating into nervous system cells
NZ524806A (en) 2000-10-23 2006-03-31 Smithkline Beecham Corp Tri-substituted 8H-pyrido[2,3-d]pyrimidin-7-one compounds
MXPA03005139A (es) 2000-12-08 2004-01-29 Ortho Mcneil Pharm Inc Compuestos macroheterociclicos utiles como inhibidores de cinasa.
ES2263681T3 (es) 2000-12-08 2006-12-16 Ortho-Mcneil Pharmaceutical, Inc. Compuestos de pirrolina indazolil-substituidos como inhibidores de la kinasa.
US6599323B2 (en) 2000-12-21 2003-07-29 Ethicon, Inc. Reinforced tissue implants and methods of manufacture and use
EP1366148A2 (en) 2001-01-24 2003-12-03 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, represented by THE DEPARTMENT OF HEALTH & HUMAN SERVICES Differentiation of stem cells to pancreatic endocrine cells
EP1355910B1 (en) 2001-01-25 2011-03-09 The United States of America, represented by the Secretary, Department of Health and Human Services Formulation of boronic acid compounds
US6656488B2 (en) 2001-04-11 2003-12-02 Ethicon Endo-Surgery, Inc. Bioabsorbable bag containing bioabsorbable materials of different bioabsorption rates for tissue engineering
EP1379626A2 (en) 2001-04-19 2004-01-14 DeveloGen Aktiengesellschaft für entwicklungsbiologische Forschung A method for differentiating stem cells into insulin-producing cells
CN100516204C (zh) 2001-04-24 2009-07-22 味之素株式会社 干细胞及其分离方法
CA2447015A1 (en) 2001-05-15 2002-11-21 Rappaport Family Institute For Research In The Medical Sciences Insulin producing cells derived from human embryonic stem cells
US6626950B2 (en) 2001-06-28 2003-09-30 Ethicon, Inc. Composite scaffold with post anchor for the repair and regeneration of tissue
KR100418195B1 (ko) 2001-07-05 2004-02-11 주식회사 우리기술 전력케이블의 다중절연진단장치 및 그 방법
GB0117583D0 (en) 2001-07-19 2001-09-12 Astrazeneca Ab Novel compounds
WO2003014313A2 (en) 2001-08-06 2003-02-20 Bresagen, Ltd. Alternative compositions and methods for the culture of stem cells
US6617152B2 (en) 2001-09-04 2003-09-09 Corning Inc Method for creating a cell growth surface on a polymeric substrate
EP1298201A1 (en) 2001-09-27 2003-04-02 Cardion AG Process for the production of cells exhibiting an islet-beta-cell-like state
CA2463914A1 (en) 2001-10-18 2003-04-24 Ixion Biotechnology, Inc. Conversion of liver stem and progenitor cells to pancreatic functional cells
US8021876B2 (en) 2001-11-15 2011-09-20 Children's Medical Center Corporation Methods of isolation, expansion and differentiation of fetal stem cells from chorionic villus, amniotic fluid, and placenta and therapeutic uses thereof
US20030161816A1 (en) 2001-12-07 2003-08-28 Fraser John K. Systems and methods for treating patients with processed lipoaspirate cells
IL162131A0 (en) 2001-12-07 2005-11-20 Geron Corp Islet cells from human embryonic stem cells
AU2002218893A1 (en) 2001-12-21 2003-07-09 Thromb-X Nv Compositions for the in vitro derivation and culture of embryonic stem (es) cell lines with germline transmission capability
IL162663A0 (en) 2001-12-28 2005-11-20 Cellartis Ab A method for the establishment of apluripotent human blastocyst-derived stem cell line
US20030162290A1 (en) 2002-01-25 2003-08-28 Kazutomo Inoue Method for inducing differentiation of embryonic stem cells into functioning cells
US20050208029A1 (en) 2002-04-17 2005-09-22 Akihiro Umezawa Method of forming pancreatic beta cells from mesenchymal cells
US20040161419A1 (en) 2002-04-19 2004-08-19 Strom Stephen C. Placental stem cells and uses thereof
ATE387444T1 (de) 2002-05-08 2008-03-15 Janssen Pharmaceutica Nv Substituierte pyrroline als kinase inhibitoren
US20060003446A1 (en) 2002-05-17 2006-01-05 Gordon Keller Mesoderm and definitive endoderm cell populations
US20060122104A1 (en) 2002-05-28 2006-06-08 Presnell Sharon C Methods for in vitro expansion and transdifferentiation of human pancreatic acinar cells into insulin-producing cells
RU2004135382A (ru) 2002-06-05 2005-06-27 Янссен Фармацевтика Н.В. (Be) Замещенные пирролины в качестве ингибиторов киназы
GB0212976D0 (en) 2002-06-06 2002-07-17 Tonejet Corp Pty Ltd Ejection method and apparatus
CN1171991C (zh) 2002-07-08 2004-10-20 徐如祥 人神经干细胞的培养方法
US6877147B2 (en) 2002-07-22 2005-04-05 Broadcom Corporation Technique to assess timing delay by use of layout quality analyzer comparison
US7838290B2 (en) 2002-07-25 2010-11-23 The Scripps Research Institute Hematopoietic stem cells and methods of treatment of neovascular eye diseases therewith
AU2003257938A1 (en) 2002-07-29 2004-02-16 Es Cell International Pte Ltd. Multi-step method for differentiation of insulin positive, glucose
US20040063204A1 (en) 2002-08-14 2004-04-01 Lijun Yang Bone marrow cell differentiation
WO2004023100A2 (en) 2002-09-06 2004-03-18 Amcyte Inc. Cd56 positive human adult pancreatic endocrine progenitor cells
US9969977B2 (en) 2002-09-20 2018-05-15 Garnet Biotherapeutics Cell populations which co-express CD49c and CD90
US20040062753A1 (en) 2002-09-27 2004-04-01 Alireza Rezania Composite scaffolds seeded with mammalian cells
US20060252150A1 (en) 2002-11-08 2006-11-09 Linzhao Cheng Human embryonic stem cell cultures, and compositions and methods for growing same
US7144999B2 (en) 2002-11-23 2006-12-05 Isis Pharmaceuticals, Inc. Modulation of hypoxia-inducible factor 1 alpha expression
US20060040385A1 (en) 2002-12-05 2006-02-23 Technion Research & Development Foundation Ltd. Cultured human pancreatic islets, and uses thereof
CN100549163C (zh) 2002-12-16 2009-10-14 技术研究及发展基金有限公司 制备无饲养细胞、无异源的人胚胎干细胞的方法以及使用该方法制备的干细胞培养物
MXPA05007883A (es) 2003-01-29 2005-09-21 Takeda Pharmaceutical Proceso para producir una preparacion recubierta.
RU2359671C2 (ru) 2003-01-29 2009-06-27 Такеда Фармасьютикал Компани Лимитед Способ получения препарата с покрытием
US20070154981A1 (en) 2003-02-14 2007-07-05 The Board Of Trustees Of The Leland Stanford Junior University Insulin-producing cells derived from stem cells
US20070155661A1 (en) 2003-02-14 2007-07-05 The Board Of Trustees Of The Leland Standord Junior University Methods and compositions for modulating the development of stem cells
US20070020242A1 (en) 2003-03-27 2007-01-25 Ixion Biotechnology, Inc. Method for transdifferentiation of non-pancreatic stem cells to the pancreatic pathway
WO2004090110A2 (en) 2003-03-31 2004-10-21 Bresagen Inc. Compositions and methods for the control, differentiation and/or manipulation of pluripotent cells through a gamma-secretase signaling pathway
US20090203141A1 (en) 2003-05-15 2009-08-13 Shi-Lung Lin Generation of tumor-free embryonic stem-like pluripotent cells using inducible recombinant RNA agents
JP5148873B2 (ja) 2003-06-27 2013-02-20 エチコン、インコーポレイテッド 臍帯組織由来の分娩後細胞、及びその作成及び使用方法
IL161903A0 (en) 2003-07-17 2005-11-20 Gamida Cell Ltd Ex vivo progenitor and stem cell expansion for usein the treatment of disease of endodermally- deri ved organs
ITRM20030395A1 (it) 2003-08-12 2005-02-13 Istituto Naz Per Le Malattie Infettive Lazz Terreno di coltura per il mantenimento, la proliferazione e il differenziamento di cellule di mammifero.
WO2005017117A2 (en) 2003-08-14 2005-02-24 Martin Haas Multipotent amniotic fetal stem cells (mafsc) and banking of same
US7157275B2 (en) 2003-08-15 2007-01-02 Becton, Dickinson And Company Peptides for enhanced cell attachment and growth
US20060205072A1 (en) 2003-08-27 2006-09-14 Nobuko Uchida Enriched pancreatic stem cell and progenitor cell populations, and methods for identifying, isolating and enriching for such populations
WO2005058301A1 (en) 2003-12-17 2005-06-30 Allergan, Inc. Methods for treating retinoid responsive disorders using selective inhibitors of cyp26a and cyp26b
US20060030042A1 (en) 2003-12-19 2006-02-09 Ali Brivanlou Maintenance of embryonic stem cells by the GSK-3 inhibitor 6-bromoindirubin-3'-oxime
CN1946838A (zh) 2003-12-23 2007-04-11 赛瑟拉公司 定形内胚层
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
US7625753B2 (en) 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
US7510876B2 (en) 2003-12-23 2009-03-31 Cythera, Inc. Definitive endoderm
TWI334443B (en) 2003-12-31 2010-12-11 Ind Tech Res Inst Method of single cell culture of undifferentiated human embryonic stem cells
US20050233446A1 (en) 2003-12-31 2005-10-20 Parsons Xuejun H Defined media for stem cell culture
WO2005071066A1 (en) 2004-01-23 2005-08-04 Board Of Regents, The University Of Texas System Methods and compositions for preparing pancreatic insulin secreting cells
US7794704B2 (en) 2004-01-23 2010-09-14 Advanced Cell Technology, Inc. Methods for producing enriched populations of human retinal pigment epithelium cells for treatment of retinal degeneration
WO2005080551A2 (en) 2004-02-12 2005-09-01 University Of Newcastle Upon Tyne Stem cells
US7964401B2 (en) 2004-02-19 2011-06-21 Kyoto University Screening method for somatic cell nuclear reprogramming substance affecting ECAT2 and ECAT3
US20060281174A1 (en) 2004-03-09 2006-12-14 Gang Xu Methods for generating insulin-producing cells
CN1950498A (zh) 2004-03-10 2007-04-18 加利福尼亚大学董事会 培养胚胎干细胞的组合物和方法
US7892835B2 (en) 2004-03-23 2011-02-22 Daiichi Sankyo Company, Limited Pluripotent stem cell growing method
WO2005097980A2 (en) 2004-03-26 2005-10-20 Geron Corporation New protocols for making hepatocytes from embryonic stem cells
JP4491014B2 (ja) 2004-04-01 2010-06-30 ウイスコンシン アラムニ リサーチ ファンデーション 幹細胞の内胚葉および膵臓系統への分化
KR101395516B1 (ko) 2004-04-27 2014-05-14 비아싸이트, 인크. Ｐｄｘ１ 발현 내배엽
JP5687816B2 (ja) 2004-07-09 2015-03-25 ヴィアサイト，インコーポレイテッド 胚体内胚葉を分化させるための因子を同定する方法
AU2005272681B2 (en) 2004-08-13 2009-11-19 University Of Georgia Research Foundation, Inc. Compositions and methods for self-renewal and differentiation in human embryonic stem cells
US20080268533A1 (en) 2004-08-25 2008-10-30 University Of Georgia Research Foundation, Inc. Methods and Compositions Utilizing Myc and Gsk3Beta to Manipulate the Pluripotency of Embryonic Stem Cells
DE102004043256B4 (de) 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
GB2432846B (en) 2004-09-08 2009-12-30 Wisconsin Alumni Res Found Medium and culture of embryonic stem cells
WO2006029198A2 (en) 2004-09-08 2006-03-16 Wisconsin Alumni Research Foundation Culturing human embryonic stem cells
EP1853698A1 (en) 2005-01-28 2007-11-14 NovaThera Ltd. Methods for embryonic stem cell culture
JP2008528038A (ja) 2005-01-31 2008-07-31 エス セル インターナショナル ピーティーイー リミテッド 胚性幹細胞の指示された分化及びその利用
US20060182724A1 (en) 2005-02-15 2006-08-17 Riordan Neil H Method for expansion of stem cells
PL1860950T3 (pl) 2005-03-04 2017-09-29 Lifescan, Inc. Dojrzałe komórki podścieliska pochodzenia trzustkowego
GB0505970D0 (en) 2005-03-23 2005-04-27 Univ Edinburgh Culture medium containing kinase inhibitor, and uses thereof
EP1876893B1 (en) 2005-04-15 2012-04-11 Geron Corporation Cancer treatment by combined inhibition of proteasome and telomerase activities
CN100425694C (zh) 2005-04-15 2008-10-15 北京大学 诱导胚胎干细胞向胰腺细胞分化的方法
US20080227656A1 (en) 2005-04-26 2008-09-18 Flemming Besenbacher Biosurface Structure Array
JP5092124B2 (ja) 2005-05-24 2012-12-05 国立大学法人 熊本大学 Ｅｓ細胞の分化誘導方法
AU2006202209B2 (en) 2005-05-27 2011-04-14 Lifescan, Inc. Amniotic fluid derived cells
EP1899344A1 (en) 2005-06-10 2008-03-19 Irm, Llc Compounds that maintain pluripotency of embryonic stem cells
WO2006138433A2 (en) 2005-06-14 2006-12-28 The Regents Of The University Of California Induction of cell differentiation by class i bhlh polypeptides
US20080199959A1 (en) 2005-06-21 2008-08-21 Ge Healthcare Bio-Sciences Ab Method For Cell Culture
GB2441488C (en) 2005-06-22 2011-10-05 Geron Corp Suspension culture of human embryonic stem cells
UA92608C2 (en) 2005-06-30 2010-11-25 Янссен Фармацевтика Н.В. Cyclic anilino - pyridinotriazines as gsk-3 inhibitors
US20080194021A1 (en) 2005-07-29 2008-08-14 Mays Robert W Use of a Gsk-3 Inhibitor to Maintain Potency of Culture Cells
AU2006274438A1 (en) 2005-07-29 2007-02-01 Australian Stem Cell Centre Limited Compositions and methods for growth of pluripotent cells
WO2007025234A2 (en) 2005-08-26 2007-03-01 The Trustees Of Columbia University In The City Of New York Generation of pancreatic endocrine cells from primary duct cell cultures and methods of use for treatment of diabetes
EP1962719A4 (en) 2005-08-29 2011-05-04 Technion Res And Dev Of Foundation Ltd MEDIA FOR BREEDING STEM CELLS
EP1937801A1 (en) 2005-09-02 2008-07-02 Agency for Science, Technology and Research Method of deriving mesenchymal stem cells
WO2007030870A1 (en) 2005-09-12 2007-03-22 Es Cell International Pte Ltd Cardiomyocyte production
WO2008048671A1 (en) 2006-10-18 2008-04-24 University Of Illinois Embryonic-like stem cells derived from adult human peripheral blood and methods of use
CN101310012B (zh) 2005-10-14 2012-05-09 明尼苏达大学董事会 非胚胎干细胞分化成具有胰腺表型的细胞
JP5404047B2 (ja) 2005-10-27 2014-01-29 ヴィアサイト，インコーポレイテッド Ｐｄｘ１発現背側及び腹側前腸内胚葉
EP2206724A1 (en) 2005-12-13 2010-07-14 Kyoto University Nuclear reprogramming factor
WO2007082963A1 (es) 2006-01-18 2007-07-26 Fundación Instituto Valenciano De Infertilidad Líneas de células madre embrionarias humanas y métodos para usar las mismas
SG10202109176RA (en) 2006-02-23 2021-09-29 Viacyte Inc Compositions and methods useful for culturing differentiable cells
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
DK2650359T3 (da) 2006-03-02 2022-06-13 Viacyte Inc Endokrine prekursorceller, pancreatiske hormon-udtrykkende celler og fremstillingsfremgangsmåder
CA2650812C (en) 2006-04-28 2017-12-12 Lifescan, Inc. Differentiation of human embryonic stem cells
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
US8685730B2 (en) 2006-05-02 2014-04-01 Wisconsin Alumni Research Foundation Methods and devices for differentiating pluripotent stem cells into cells of the pancreatic lineage
AU2007248609B2 (en) 2006-05-02 2012-11-01 Wisconsin Alumni Research Foundation Method of differentiating stem cells into cells of the endoderm and pancreatic lineage
US7964402B2 (en) 2006-05-25 2011-06-21 Sanford-Burnham Medical Research Institute Methods for culture and production of single cell populations of human embryonic stem cells
AU2007254766A1 (en) 2006-06-02 2007-12-13 University Of Georgia Research Foundation, Inc. Pancreatic and liver endoderm cells and tissue by differentiation of definitive endoderm cells obtained from human embryonic stems
CN101541953A (zh) 2006-06-02 2009-09-23 佐治亚大学研究基金会 通过从人胚胎干细胞获得的定形内胚层细胞的分化得到胰和肝内胚层细胞及组织
US8415153B2 (en) * 2006-06-19 2013-04-09 Geron Corporation Differentiation and enrichment of islet-like cells from human pluripotent stem cells
CN100494359C (zh) * 2006-06-23 2009-06-03 中日友好医院 神经干细胞三维立体培养体外扩增的方法
WO2008036447A2 (en) 2006-06-26 2008-03-27 Lifescan, Inc. Pluripotent stem cell culture
US20080003676A1 (en) 2006-06-26 2008-01-03 Millipore Corporation Growth of embryonic stem cells
WO2008004990A2 (en) * 2006-07-06 2008-01-10 Es Cell International Pte Ltd Method for stem cell culture and cells derived therefrom
WO2008013664A2 (en) 2006-07-26 2008-01-31 Cythera, Inc. Methods of producing pancreatic hormones
DK3441459T3 (da) 2006-08-02 2021-06-07 Technion Res & Dev Foundation Fremgangsmåder til ekspansion af embryonale stamceller i en suspensionskultur
KR101331510B1 (ko) 2006-08-30 2013-11-20 재단법인서울대학교산학협력재단 저농도의 포도당을 함유하는 인간 배아줄기세포용 배지조성물 및 이를 이용한 인간 배아 줄기세포로부터 인슐린생산 세포 또는 세포괴로 분화시키는 방법, 그리고그로부터 유도된 인슐린 생산 세포 또는 세포괴
JP2008099662A (ja) * 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
US20080091234A1 (en) 2006-09-26 2008-04-17 Kladakis Stephanie M Method for modifying a medical implant surface for promoting tissue growth
KR101419965B1 (ko) 2006-10-17 2014-07-16 스티펠 래버러토리즈, 인코포레이티드 탈라로졸 대사물
US20100323442A1 (en) 2006-10-17 2010-12-23 Emmanuel Edward Baetge Modulation of the phosphatidylinositol-3-kinase pathway in the differentiation of human embryonic stem cells
RU2323252C1 (ru) * 2006-10-25 2008-04-27 Антонина Ивановна Колесникова Способ культивирования мезенхимальных стволовых клеток человека ex vivo
JP5067949B2 (ja) 2006-11-09 2012-11-07 独立行政法人国立国際医療研究センター 霊長類動物胚性幹細胞の培養及び継代方法、並びにその分化誘導方法
WO2008086005A1 (en) 2007-01-09 2008-07-17 University Of South Florida Compositions including triciribine and bortezomib and derivatives thereof and methods of use thereof
WO2008089351A1 (en) * 2007-01-17 2008-07-24 Wisconsin Alumni Research Foundation Improved culture of stem cells
US9175260B2 (en) 2007-01-30 2015-11-03 TheUniversity of Georgia Research Foundation, Inc. Early mesoderm cells, a stable population of mesendoderm cells that has utility for generation of endoderm and mesoderm lineages and multipotent migratory cells (MMC)
GB0703188D0 (en) * 2007-02-19 2007-03-28 Roger Land Building Large scale production of stem cells
US20090053182A1 (en) 2007-05-25 2009-02-26 Medistem Laboratories, Inc. Endometrial stem cells and methods of making and using same
DK2173863T3 (en) 2007-06-29 2019-01-21 Fujifilm Cellular Dynamics Inc Automated method and apparatus for embryonic stem cell culture
CN105176919A (zh) 2007-07-18 2015-12-23 生命扫描有限公司 人胚胎干细胞的分化
RU2473685C2 (ru) 2007-07-31 2013-01-27 Лайфскен, Инк. Дифференцировка человеческих эмбриональных стволовых клеток
ES2648128T3 (es) 2007-07-31 2017-12-28 Lifescan, Inc. Diferenciación de células madre pluripotentes usando células alimentadoras humanas
AU2008291930B2 (en) 2007-08-24 2014-04-17 Slotervaart Participaties Bv Compositions for the treatment of neoplastic diseases
US20110151447A1 (en) 2007-11-06 2011-06-23 Children's Medical Center Corporation Method to produce induced pluripotent stem (ips) cells from non-embryonic human cells
CN101878298B (zh) 2007-11-27 2017-08-15 生命扫描有限公司 人胚胎干细胞的分化
SG154367A1 (en) 2008-01-31 2009-08-28 Es Cell Int Pte Ltd Method of differentiating stem cells
WO2009096049A1 (ja) 2008-02-01 2009-08-06 Kyoto University 人工多能性幹細胞由来分化細胞
EP2250252A2 (en) 2008-02-11 2010-11-17 Cambridge Enterprise Limited Improved reprogramming of mammalian cells, and the cells obtained
CN105886459A (zh) * 2008-02-21 2016-08-24 詹森生物科技公司 用于细胞粘附、培养和分离的方法、表面改性培养板和组合物
JPWO2009110215A1 (ja) 2008-03-03 2011-07-14 独立行政法人科学技術振興機構 繊毛細胞の分化誘導方法
JP2011514169A (ja) 2008-03-17 2011-05-06 エージェンシー フォー サイエンス，テクノロジー アンド リサーチ 幹細胞培養のためのマイクロキャリア
US8338170B2 (en) 2008-04-21 2012-12-25 Viacyte, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
AU2008355123B2 (en) 2008-04-21 2014-12-04 Viacyte, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
US8728812B2 (en) 2008-04-22 2014-05-20 President And Fellows Of Harvard College Compositions and methods for promoting the generation of PDX1+ pancreatic cells
US7939322B2 (en) 2008-04-24 2011-05-10 Centocor Ortho Biotech Inc. Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm
US8623648B2 (en) 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
DK2993226T3 (da) 2008-06-03 2021-02-22 Viacyte Inc Vækstfaktorer til fremstilling af en definitiv endoderm
US20090298178A1 (en) 2008-06-03 2009-12-03 D Amour Kevin Allen Growth factors for production of definitive endoderm
KR101829310B1 (ko) 2008-06-30 2018-02-14 얀센 바이오테크 인코포레이티드 만능 줄기 세포의 분화
DE102008032236A1 (de) 2008-06-30 2010-04-01 Eberhard-Karls-Universität Tübingen Isolierung und/oder Identifizierung von Stammzellen mit adipozytärem, chondrozytärem und pankreatischem Differenzierungspotential
US20100028307A1 (en) 2008-07-31 2010-02-04 O'neil John J Pluripotent stem cell differentiation
WO2010022395A2 (en) 2008-08-22 2010-02-25 President And Fellows Of Harvard College Methods of reprogramming cells
EP2350265B1 (en) 2008-10-31 2019-04-17 Janssen Biotech, Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
ES2634445T3 (es) 2008-10-31 2017-09-27 Janssen Biotech, Inc. Diferenciación de las células madre embrionarias humanas con el linaje endocrino pancreático
NZ592622A (en) 2008-11-04 2012-10-26 Viacyte Inc Stem cell aggregate suspension compositions and methods for differentiation thereof
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
CN105349517B (zh) 2008-11-14 2021-05-04 维赛特公司 源于人多能干细胞的胰腺细胞的包封
US20100124781A1 (en) 2008-11-20 2010-05-20 Shelley Nelson Pluripotent Stem Cell Culture on Micro-Carriers
EP2356218B1 (en) 2008-12-05 2017-05-03 INSERM (Institut National de la Santé et de la Recherche Médicale) Method and medium for neural differentiation of pluripotent cells
RU2579278C2 (ru) 2009-07-20 2016-04-10 Янссен Байотек, Инк. Популяция панкреатических эндокринных клеток-предшественников для снижения концентрации глюкозы в крови и способ дифференцировки панкреатических эндодермальных клеток
CN102597219B (zh) 2009-07-20 2015-08-19 詹森生物科技公司 人胚胎干细胞的分化
JP5957382B2 (ja) 2009-10-29 2016-07-27 ヤンセン バイオテツク，インコーポレーテツド 多能性幹細胞
FI20096288A0 (fi) 2009-12-04 2009-12-04 Kristiina Rajala Formulations and methods for culturing stem cells
EP2516626B1 (en) 2009-12-23 2017-05-10 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
CA2784415C (en) 2009-12-23 2019-06-18 Jean Xu Differentiation of human embryonic stem cells
SG10201501513TA (en) 2010-03-02 2015-04-29 Univ Singapore Culture additives to boost stem cell proliferation and differentiation response
AU2011235212B2 (en) 2010-03-31 2014-07-31 The Scripps Research Institute Reprogramming cells
US9234170B2 (en) 2010-04-25 2016-01-12 Mount Sinai School Of Medicine Generation of anterior foregut endoderm from pluripotent cells
EP3498825A1 (en) 2010-05-12 2019-06-19 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
DK2601288T3 (en) 2010-08-05 2016-05-30 Wisconsin Alumni Res Found Simplified base media for human pluripotent cell culture
CA2809305C (en) 2010-08-31 2019-06-11 Janssen Biotech, Inc. Differentiation of pluripotent stem cells
MY177150A (en) 2011-02-28 2020-09-08 Stempeutics Res Malaysia Sdn Bhd Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof
WO2013055834A2 (en) 2011-10-11 2013-04-18 The New York Stem Cell Foundation Er stress relievers in beta cell protection
US9670463B2 (en) 2011-10-14 2017-06-06 Children's Medical Center Corporation Inhibition and enhancement of reprogramming by chromatin modifying enzymes
RU2705001C2 (ru) 2011-12-22 2019-11-01 Янссен Байотек, Инк. Дифференцировка эмбриональных стволовых клеток человека в одногормональные инсулинположительные клетки
US10519422B2 (en) 2012-02-29 2019-12-31 Riken Method of producing human retinal pigment epithelial cells
AU2013271581B2 (en) 2012-06-08 2018-08-09 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endocrine cells
EP2893000B1 (en) 2012-09-03 2019-04-10 Novo Nordisk A/S Generation of pancreatic endoderm from pluripotent stem cells using small molecules
BR112015015701A2 (pt) 2012-12-31 2017-07-11 Janssen Biotech Inc diferenciação de células-tronco embrionárias humanas em células pancreáticas endócrinas com o uso de reguladores hb9
US8859286B2 (en) 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
CA2906643A1 (en) 2013-03-15 2014-09-25 The Jackson Laboratory Isolation of non-embryonic stem cells and uses thereof
US11645208B2 (en) 2021-03-29 2023-05-09 International Business Machines Corporation Translation bandwidth optimized prefetching strategy through multiple translation lookaside buffers

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