RU2011124900A - Культура плюрипотентных стволовых клеток на микроносителях - Google Patents
Культура плюрипотентных стволовых клеток на микроносителях Download PDFInfo
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- 210000001778 pluripotent stem cell Anatomy 0.000 title claims abstract 26
- 239000000969 carrier Substances 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract 19
- 210000004027 cell Anatomy 0.000 claims abstract 7
- 229920002307 Dextran Polymers 0.000 claims abstract 4
- 239000004793 Polystyrene Substances 0.000 claims abstract 4
- 230000002255 enzymatic effect Effects 0.000 claims abstract 4
- 229920002223 polystyrene Polymers 0.000 claims abstract 4
- 239000003590 rho kinase inhibitor Substances 0.000 claims abstract 4
- 238000012258 culturing Methods 0.000 claims abstract 3
- 210000001900 endoderm Anatomy 0.000 claims 4
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 4
- 230000024245 cell differentiation Effects 0.000 claims 3
- 102000004877 Insulin Human genes 0.000 claims 2
- 108090001061 Insulin Proteins 0.000 claims 2
- 210000003890 endocrine cell Anatomy 0.000 claims 2
- 229940125396 insulin Drugs 0.000 claims 2
- 230000009996 pancreatic endocrine effect Effects 0.000 claims 2
- 230000004069 differentiation Effects 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
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Abstract
1. Способ выращивания плюрипотентных стволовых клеток, включающий следующие этапы:а. прикрепление популяции плюрипотентных стволовых клеток к первому объему микроносителей;b. культивирование плюрипотентных стволовых клеток на первом объеме микроносителей;c. отделение плюрипотентных стволовых клеток от первого объема микроносителей;d. прикрепление популяции плюрипотентных стволовых клеток ко второму объему микроносителей.2. Способ по п.1, в котором этапы культивирования, отделения и прикрепления плюрипотентных стволовых клеток на микроносителях повторяются с использованием следующих объемов микроносителей.3. Способ по п.1, в котором первый объем микроносителей выбирается из группы, состоящей из декстрановых микроносителей и полистироловых микроносителей.4. Способ по п.1, в котором второй объем микроносителей выбирается из группы, состоящей из декстрановых микроносителей и полистироловых микроносителей.5. Способ по п.1, в котором плюрипотентные стволовые клетки прикрепляются к первому объему микроносителей в среде, содержащей ингибитор Rho-киназы.6. Способ по п.1, в котором плюрипотентные стволовые клетки прикрепляются ко второму объему микроносителей в среде, содержащей ингибитор Rho-киназы.7. Способ по п.1, в котором плюрипотентные стволовые клетки отделяются от первого объема микроносителей путем ферментативной обработки.8. Способ по п.1, в котором плюрипотентные стволовые клетки отделяются от второго объема микроносителей путем ферментативной обработки.9. Способ по п.1, в котором первый объем микроносителей отделяется от плюрипотентных стволовых клеток перед прикреплением клеток ко второму объему микро
Claims (10)
1. Способ выращивания плюрипотентных стволовых клеток, включающий следующие этапы:
а. прикрепление популяции плюрипотентных стволовых клеток к первому объему микроносителей;
b. культивирование плюрипотентных стволовых клеток на первом объеме микроносителей;
c. отделение плюрипотентных стволовых клеток от первого объема микроносителей;
d. прикрепление популяции плюрипотентных стволовых клеток ко второму объему микроносителей.
2. Способ по п.1, в котором этапы культивирования, отделения и прикрепления плюрипотентных стволовых клеток на микроносителях повторяются с использованием следующих объемов микроносителей.
3. Способ по п.1, в котором первый объем микроносителей выбирается из группы, состоящей из декстрановых микроносителей и полистироловых микроносителей.
4. Способ по п.1, в котором второй объем микроносителей выбирается из группы, состоящей из декстрановых микроносителей и полистироловых микроносителей.
5. Способ по п.1, в котором плюрипотентные стволовые клетки прикрепляются к первому объему микроносителей в среде, содержащей ингибитор Rho-киназы.
6. Способ по п.1, в котором плюрипотентные стволовые клетки прикрепляются ко второму объему микроносителей в среде, содержащей ингибитор Rho-киназы.
7. Способ по п.1, в котором плюрипотентные стволовые клетки отделяются от первого объема микроносителей путем ферментативной обработки.
8. Способ по п.1, в котором плюрипотентные стволовые клетки отделяются от второго объема микроносителей путем ферментативной обработки.
9. Способ по п.1, в котором первый объем микроносителей отделяется от плюрипотентных стволовых клеток перед прикреплением клеток ко второму объему микроносителей.
10. Способ дифференцирования плюрипотентных стволовых клеток в инсулинэкспрессирующие клетки, состоящий из следующих этапов:
а. прикрепление плюрипотентных стволовых клеток к объему микроносителей;
b. дифференцирование плюрипотентных стволовых клеток в клетки, экспрессирующие маркеры, характерные для линии сформированной эндодермы;
c. дифференцирование клеток, экспрессирующих маркеры, характерные для линии сформированной эндодермы, в клетки, экспрессирующие маркеры, характерные для линии панкреатической эндодермы;
d. дифференцирование клеток, экспрессирующих маркеры, характерные для линии панкреатической эндодермы, в клетки, экспрессирующие маркеры, характерные для линии панкреатических эндокринных клеток;
e. дифференцирование клеток, экспрессирующих маркеры, характерные для линии панкреатических эндокринных клеток, в инсулинэкспрессирующие клетки.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11644708P | 2008-11-20 | 2008-11-20 | |
| US61/116,447 | 2008-11-20 | ||
| PCT/US2009/065062 WO2010059775A1 (en) | 2008-11-20 | 2009-11-19 | Pluripotent stem cell culture on micro-carriers |
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| Publication Number | Publication Date |
|---|---|
| RU2011124900A true RU2011124900A (ru) | 2012-12-27 |
| RU2555538C2 RU2555538C2 (ru) | 2015-07-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| RU2011124900/10A RU2555538C2 (ru) | 2008-11-20 | 2009-11-19 | Культура плюрипотентных стволовых клеток на микроносителях |
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| Country | Link |
|---|---|
| US (3) | US20100124781A1 (ru) |
| EP (2) | EP2366021B1 (ru) |
| JP (1) | JP2012509085A (ru) |
| KR (2) | KR101774546B1 (ru) |
| CN (2) | CN102307991B (ru) |
| AU (2) | AU2009316580B2 (ru) |
| BR (1) | BRPI0920956A2 (ru) |
| CA (1) | CA2744234C (ru) |
| ES (1) | ES2642070T3 (ru) |
| HK (1) | HK1246356A1 (ru) |
| MX (2) | MX2011005288A (ru) |
| PL (1) | PL2366021T3 (ru) |
| RU (1) | RU2555538C2 (ru) |
| WO (1) | WO2010059775A1 (ru) |
| ZA (2) | ZA201104506B (ru) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100381768B1 (ko) * | 2001-08-21 | 2003-04-26 | 김승환 | 무선 에이티엠망의 동적 자원할당 매체접속제어 프로토콜 |
| US9080145B2 (en) | 2007-07-01 | 2015-07-14 | Lifescan Corporation | Single pluripotent stem cell culture |
| EP2185693B1 (en) | 2007-07-31 | 2019-07-03 | Lifescan, Inc. | Differentiation of human embryonic stem cells |
| RU2473684C2 (ru) | 2007-11-27 | 2013-01-27 | Лайфскен, Инк. | Дифференцировка человеческих эмбриональных стволовых клеток |
| BRPI0908033A2 (pt) | 2008-02-21 | 2015-08-04 | Centocor Ortho Biotech Inc | Método placas de superfície modificada e composições para adesão, cultura e desprendimento de célula |
| US8828720B2 (en) * | 2008-03-17 | 2014-09-09 | Agency For Science, Technology And Research | Microcarriers for stem cell culture |
| US20110143433A1 (en) * | 2008-03-17 | 2011-06-16 | Agency For Science, Technology And Research | Microcarriers for Stem Cell Culture |
| US8691569B2 (en) | 2008-03-17 | 2014-04-08 | Agency For Science, Technology And Research | Microcarriers for stem cell culture |
| WO2009116951A2 (en) * | 2008-03-17 | 2009-09-24 | Agency For Science, Technology And Research | Microcarriers for stem cell culture |
| US9458431B2 (en) | 2008-03-17 | 2016-10-04 | Agency For Science, Technology And Research | Microcarriers for stem cell culture |
| US20100015711A1 (en) | 2008-06-30 | 2010-01-21 | Janet Davis | Differentiation of Pluripotent Stem Cells |
| WO2010051223A1 (en) | 2008-10-31 | 2010-05-06 | Centocor Ortho Biotech Inc. | Differentiation of human embryonic stem cells to the pancreatic endocrine lineage |
| AU2009309044B2 (en) | 2008-10-31 | 2015-07-09 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells to the pancreatic endocrine lineage |
| RU2547925C2 (ru) | 2008-11-20 | 2015-04-10 | Сентокор Орто Байотек Инк. | Способы и композиции для закрепления и культивирования клеток на плоских носителях |
| KR101774546B1 (ko) | 2008-11-20 | 2017-09-04 | 얀센 바이오테크 인코포레이티드 | 마이크로-캐리어 상의 만능 줄기 세포 배양 |
| SG174471A1 (en) | 2009-03-20 | 2011-10-28 | Agency Science Tech & Res | Culture of pluripotent and multipotent cells on microcarriers |
| BR112012001480A2 (pt) * | 2009-07-20 | 2015-09-01 | Janssen Biotech Inc | Diferenciação de células-tronco embriônicas humanas |
| CN102741395B (zh) | 2009-12-23 | 2016-03-16 | 詹森生物科技公司 | 人胚胎干细胞的分化 |
| RU2702198C2 (ru) | 2010-03-01 | 2019-10-04 | Янссен Байотек, Инк. | Способы очистки клеток, производных от плюрипотентных стволовых клеток |
| CA2800610C (en) | 2010-05-12 | 2019-09-24 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
| US20120156246A1 (en) * | 2010-06-16 | 2012-06-21 | Bamdad Cynthia C | Reprogramming cancer cells |
| BR112013004614A2 (pt) | 2010-08-31 | 2024-01-16 | Janssen Biotech Inc | Diferenciação de células-tronco pluripotentes |
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- 2009-11-19 PL PL09759851T patent/PL2366021T3/pl unknown
- 2009-11-19 CN CN200980155382.2A patent/CN102307991B/zh not_active Expired - Fee Related
- 2009-11-19 ES ES09759851.0T patent/ES2642070T3/es active Active
- 2009-11-19 US US12/621,686 patent/US20100124781A1/en not_active Abandoned
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- 2009-11-19 BR BRPI0920956-5A patent/BRPI0920956A2/pt not_active Application Discontinuation
- 2009-11-19 EP EP17184333.7A patent/EP3260534A1/en not_active Withdrawn
- 2009-11-19 RU RU2011124900/10A patent/RU2555538C2/ru not_active IP Right Cessation
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- 2009-11-19 WO PCT/US2009/065062 patent/WO2010059775A1/en active Application Filing
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2011
- 2011-06-17 ZA ZA2011/04506A patent/ZA201104506B/en unknown
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2012
- 2012-02-27 HK HK18105925.9A patent/HK1246356A1/zh unknown
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2015
- 2015-02-25 US US14/631,019 patent/US9969972B2/en not_active Expired - Fee Related
-
2016
- 2016-02-26 AU AU2016201220A patent/AU2016201220B2/en not_active Ceased
- 2016-09-16 ZA ZA2016/06403A patent/ZA201606403B/en unknown
-
2018
- 2018-05-14 US US15/978,913 patent/US20180265842A1/en not_active Abandoned
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| Publication number | Publication date |
|---|---|
| US20150166950A1 (en) | 2015-06-18 |
| CA2744234C (en) | 2018-11-06 |
| JP2012509085A (ja) | 2012-04-19 |
| US20100124781A1 (en) | 2010-05-20 |
| ES2642070T3 (es) | 2017-11-15 |
| ZA201606403B (en) | 2018-05-30 |
| CN102307991A (zh) | 2012-01-04 |
| CN102307991B (zh) | 2017-08-15 |
| KR101774546B1 (ko) | 2017-09-04 |
| EP3260534A1 (en) | 2017-12-27 |
| PL2366021T3 (pl) | 2017-12-29 |
| EP2366021B1 (en) | 2017-08-02 |
| WO2010059775A1 (en) | 2010-05-27 |
| RU2555538C2 (ru) | 2015-07-10 |
| US20180265842A1 (en) | 2018-09-20 |
| AU2016201220B2 (en) | 2017-09-28 |
| CA2744234A1 (en) | 2010-05-27 |
| MX356756B (es) | 2018-06-11 |
| AU2016201220A1 (en) | 2016-03-17 |
| CN107267442A (zh) | 2017-10-20 |
| MX2011005288A (es) | 2011-06-01 |
| BRPI0920956A2 (pt) | 2015-08-18 |
| EP2366021A1 (en) | 2011-09-21 |
| KR101837080B1 (ko) | 2018-03-09 |
| HK1246356A1 (zh) | 2018-09-07 |
| KR20110091768A (ko) | 2011-08-12 |
| AU2009316580A1 (en) | 2010-05-27 |
| US9969972B2 (en) | 2018-05-15 |
| KR20170102075A (ko) | 2017-09-06 |
| ZA201104506B (en) | 2018-11-28 |
| AU2009316580B2 (en) | 2016-04-14 |
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