ATE365793T1 - Verfahren zur isolierung und kulturexpansion mesenchymaler stamm-/vorläuferzellen aus nabelschnurblut sowie verfahren zur differenzierung von aus nabelschnurblut stammenden mesenchymalen stamm-/vorläuferzellen in unterschiedliche mesenchymgewebe - Google Patents

Verfahren zur isolierung und kulturexpansion mesenchymaler stamm-/vorläuferzellen aus nabelschnurblut sowie verfahren zur differenzierung von aus nabelschnurblut stammenden mesenchymalen stamm-/vorläuferzellen in unterschiedliche mesenchymgewebe

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Publication number
ATE365793T1
ATE365793T1 AT03705473T AT03705473T ATE365793T1 AT E365793 T1 ATE365793 T1 AT E365793T1 AT 03705473 T AT03705473 T AT 03705473T AT 03705473 T AT03705473 T AT 03705473T AT E365793 T1 ATE365793 T1 AT E365793T1
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Austria
Prior art keywords
cells
cord blood
mesenchymal stem
progressor
umbilical cord
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AT03705473T
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English (en)
Inventor
Chul-Won Ha
Yoon-Sun Yang
Sung-Eun Yang
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Medipost Co Ltd
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Publication of ATE365793T1 publication Critical patent/ATE365793T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/42—Organic phosphate, e.g. beta glycerophosphate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT03705473T 2002-02-19 2003-02-19 Verfahren zur isolierung und kulturexpansion mesenchymaler stamm-/vorläuferzellen aus nabelschnurblut sowie verfahren zur differenzierung von aus nabelschnurblut stammenden mesenchymalen stamm-/vorläuferzellen in unterschiedliche mesenchymgewebe ATE365793T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR20020008639 2002-02-19

Publications (1)

Publication Number Publication Date
ATE365793T1 true ATE365793T1 (de) 2007-07-15

Family

ID=27751895

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03705473T ATE365793T1 (de) 2002-02-19 2003-02-19 Verfahren zur isolierung und kulturexpansion mesenchymaler stamm-/vorläuferzellen aus nabelschnurblut sowie verfahren zur differenzierung von aus nabelschnurblut stammenden mesenchymalen stamm-/vorläuferzellen in unterschiedliche mesenchymgewebe

Country Status (11)

Country Link
US (2) US20050118714A1 (de)
EP (1) EP1483371B1 (de)
JP (1) JP2005517441A (de)
KR (1) KR100489248B1 (de)
CN (1) CN1281739C (de)
AT (1) ATE365793T1 (de)
AU (1) AU2003207399A1 (de)
DE (1) DE60314602T2 (de)
DK (1) DK1483371T3 (de)
ES (1) ES2287447T3 (de)
WO (1) WO2003070922A1 (de)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7560280B2 (en) * 2000-11-03 2009-07-14 Kourion Therapeutics Gmbh Human cord blood derived unrestricted somatic stem cells (USSC)
US20040115804A1 (en) * 2002-12-13 2004-06-17 Yu-Show Fu Cell system for generating somatic cells
US7875272B2 (en) 2003-06-27 2011-01-25 Ethicon, Incorporated Treatment of stroke and other acute neuraldegenerative disorders using postpartum derived cells
US9592258B2 (en) 2003-06-27 2017-03-14 DePuy Synthes Products, Inc. Treatment of neurological injury by administration of human umbilical cord tissue-derived cells
US9572840B2 (en) 2003-06-27 2017-02-21 DePuy Synthes Products, Inc. Regeneration and repair of neural tissue using postpartum-derived cells
PL1641914T3 (pl) 2003-06-27 2017-01-31 DePuy Synthes Products, Inc. Komórki pochodzące z poporodowej tkanki łożyska oraz sposoby uzyskiwania i zastosowania tych komórek
US8790637B2 (en) 2003-06-27 2014-07-29 DePuy Synthes Products, LLC Repair and regeneration of ocular tissue using postpartum-derived cells
WO2005040360A1 (en) * 2003-10-14 2005-05-06 Universitätsklinikum Hamburg-Eppendorf Blood products from mesenchymal stem cells
JP2007520462A (ja) * 2003-12-19 2007-07-26 バイアセル インコーポレーティッド ヒト臍帯血由来多能性細胞の疾患の処置のための使用方法
EP1544290A1 (de) * 2003-12-19 2005-06-22 Yu-Show Fu System zur Herstellung somatischer Zellen
EP1711598A4 (de) * 2004-01-30 2009-04-08 Lifecord Inc Verfahren zur isolierung und kultivierung multipotenter vorläufer-/stammzellen aus nabelschnurblut sowie verfahren zur induktion ihrer differenzierung
WO2006015214A2 (en) * 2004-07-29 2006-02-09 Steenblock Research Institute, Inc. Umbilical cord stem cell composition & method of treating neurological diseases
DK1789534T3 (en) 2004-08-16 2014-11-17 Cellres Corp Pte Ltd ISOLATION OF stem / progenitor cells FOSTER HINDE FROM an umbilical cord
US20060045872A1 (en) * 2004-08-25 2006-03-02 Universidad Autonoma De Madrid Ciudad Universitaria de Cantoblanco Use of adipose tissue-derived stromal stem cells in treating fistula
CA2592435C (en) 2004-12-23 2017-03-28 Ethicon, Incorporated Treatment of stroke and other acute neural degenerative disorders using postpartum derived cells
CN101227928A (zh) * 2005-05-27 2008-07-23 华沙整形外科股份有限公司 软骨形成组合物及其使用方法
GB0511723D0 (en) * 2005-06-09 2005-07-13 Smith & Nephew Placental stem cells
US20070041948A1 (en) * 2005-07-20 2007-02-22 Seoul National University Industry Foundation Method for culturing and proliferating hematopoietic stem cells and progenitor cells using human endometrial cells
DE602005017812D1 (de) * 2005-08-26 2009-12-31 Seoul Nat Univ Ind Foundation Multipotente blutstammzellen aus der nabelschnur und zellbehandlungsmittel damit zur behandlung der ischämischen krankheit
EP1767617A1 (de) * 2005-09-26 2007-03-28 Letizia Mazzini Isolierung und Vermehrung mesenchymaler Stammzellen
US8287854B2 (en) 2005-10-21 2012-10-16 Cellresearch Corporation Pte Ltd Skin equivalents derived from umbilical cord mesenchymal stem/progenitor cells and umbilical cord epithelial stem/progenitor cells
KR100697326B1 (ko) * 2005-12-02 2007-03-20 재단법인서울대학교산학협력재단 Ｏｃｔ４ 발현능을 가지는 제대혈 유래 다분화능 성체줄기세포 및 그의 제조방법
CN101374946B (zh) 2005-12-16 2017-07-18 伊西康公司 用于在组织相容性不匹配的移植中抑制有害的免疫反应的组合物和方法
US9125906B2 (en) 2005-12-28 2015-09-08 DePuy Synthes Products, Inc. Treatment of peripheral vascular disease using umbilical cord tissue-derived cells
KR20180105266A (ko) * 2005-12-29 2018-09-27 안트로제네시스 코포레이션 태반 줄기 세포 집단
US20070178073A1 (en) * 2006-02-01 2007-08-02 Samsung Life Public Welfare Foundation Composition Comprising Separated or Proliferated Cells from Umbilical Cord Blood for Treating Developmental and/or Chronic Lung Disease
WO2007089102A1 (en) * 2006-02-01 2007-08-09 Samsungn Life Public Welfare Foundation Composition for treating developmental and/or chronic lung diseases comprising cells separated or proliferated from umbilical cord blood
WO2007114740A2 (fr) * 2006-04-04 2007-10-11 Trans-Technologies Ltd Transplant biologique destiné à la thérapie cellulaire à base de cellules souches mésenchymateuses, obtenues à partir de la moelle osseuse
ITTO20060282A1 (it) * 2006-04-14 2007-10-15 Univ Degli Studi Torino Mezzo di coltura e composizione farmaceutica per la rigenerazione del tessuto cartilagineo relativo procedimento relativi usi e prodotti
CN100453640C (zh) * 2006-04-29 2009-01-21 中国医学科学院血液学研究所 一种从脐带血中分离多能成体祖细胞的方法
JPWO2007129428A1 (ja) * 2006-05-02 2009-12-24 株式会社ステリック再生医科学研究所 幹細胞の単離方法
WO2007136673A2 (en) * 2006-05-19 2007-11-29 Medistem Laboratories, Inc. Treatment of disc degenerative disease and compositions for same
KR100791487B1 (ko) 2006-05-29 2008-01-03 연세대학교 산학협력단 제대혈로부터 간엽줄기세포의 분리 및 배양 방법
US20070298497A1 (en) * 2006-06-26 2007-12-27 Gambro Bct, Inc. Method of Culturing Mesenchymal Stem Cells
KR100812544B1 (ko) * 2006-10-19 2008-03-13 재단법인서울대학교산학협력재단 제대혈 유래 다분화능 줄기세포 및 이를 함유하는 허혈성괴사질환 치료용 세포치료제
PL2089511T3 (pl) 2006-11-13 2015-02-27 Depuy Synthes Products Llc Rozrost in vitro komórek pochodzących z tkanki pępowinowej wykorzystujących mikronośniki
KR100902569B1 (ko) 2007-01-19 2009-06-11 재단법인서울대학교산학협력재단 인간 탯줄 유래 중간엽 줄기세포 및 이의 확립방법
US9387226B2 (en) 2006-11-30 2016-07-12 Medipost Co., Ltd Neural cell proliferation induced through the culture of neural cells with umbilical cord blood-derived mesenchymal stem cells
AR065584A1 (es) * 2007-03-01 2009-06-17 Cryo Cell Internat Inc Obtencion aislamiento y crioconservacion de celulas endometriales / menstruales
KR101039235B1 (ko) 2007-08-29 2011-06-07 메디포스트(주) 제대혈 유래 간엽줄기세포를 포함하는 인터루킨-8 또는지알오-알파 발현 세포가 관련된 질병의 진단, 예방 또는치료용 조성물
PT2200622E (pt) * 2007-09-19 2012-11-06 Pluristem Ltd Células aderentes provenientes de tecido adiposo ou placentário e respetiva utilização terapêutica
CN101543644B (zh) * 2008-03-27 2012-07-25 中国人民解放军总医院 无支架工程软骨组织的构建方法及其产品
ES2872337T3 (es) * 2008-06-25 2021-11-02 Mesoblast Inc Células STRO-1bright para su uso en el tratamiento del dolor de espalda baja
KR20100054711A (ko) 2008-11-14 2010-05-25 메디포스트(주) 간엽 줄기세포 또는 이의 배양액을 포함하는 신경질환의 예방 또는 치료용 조성물
US10179900B2 (en) 2008-12-19 2019-01-15 DePuy Synthes Products, Inc. Conditioned media and methods of making a conditioned media
ES2665883T3 (es) 2008-12-19 2018-04-30 DePuy Synthes Products, Inc. Tratamiento de enfermedades y trastornos pulmonares y pulmonares
DE102009053519B4 (de) * 2009-02-27 2013-05-16 Ulrike Haas Verfahren zur Gewinnung von Myofibroblasten zur Herstellung von zur Transplantation geeignetem Gewebe
EP2408904B1 (de) * 2009-03-20 2017-10-18 Mesoblast, Inc. Herstellung von umprogrammierten pluripotenten zellen
EP2411504B1 (de) 2009-03-26 2017-05-10 DePuy Synthes Products, Inc. Humane nabelschnur gewebezellen in der alzheimer therapie
WO2010131917A2 (ko) * 2009-05-13 2010-11-18 메디포스트(주) 줄기세포의 세포 활성과 연관된 ｔｓｐ-1, ｔｓｐ-2, ｉｌ-17ｂｒ 및 ｈｂ-ｅｇｆ 및 이들의 용도
US9040298B2 (en) 2009-05-29 2015-05-26 Medipost Co., Ltd. Method of selecting stem cells having high chondrogenic differentiation capability
KR101289535B1 (ko) 2009-12-07 2013-07-24 전민용 원심분리관
CN102198156A (zh) * 2010-03-26 2011-09-28 傅毓秀 用于治疗皮肤创伤之医药组合物
WO2012048276A2 (en) 2010-10-08 2012-04-12 Caridianbct, Inc. Customizable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
KR101615161B1 (ko) 2011-02-02 2016-04-25 메디포스트(주) 신경질환의 예방 또는 치료를 위한 icam-1의 용도
CN102250837B (zh) * 2011-06-28 2013-03-06 江苏省北科生物科技有限公司 脐带间充质干细胞数字化自动生产方法
AU2012279995C1 (en) 2011-07-06 2019-10-24 Cell Therapy Limited Progenitor cells of mesodermal lineage
EP2794854B1 (de) 2011-12-23 2018-06-20 DePuy Synthes Products, Inc. Nachweis von zellen aus menschlichem nabelschnurgewebe
US8940294B2 (en) 2012-03-02 2015-01-27 Tissuetech, Inc. Methods of isolating and culturing stem cells
KR101532556B1 (ko) 2012-09-03 2015-06-30 메디포스트(주) 간엽줄기세포의 배양 방법
JP6313327B2 (ja) * 2012-12-12 2018-04-18 メソブラスト、インコーポレイテッド 呼吸器系疾患を治療又は予防する方法
WO2014135949A2 (en) * 2013-03-04 2014-09-12 Ranjith Kumar Novel method of progenitor cell expansion
WO2015073918A1 (en) 2013-11-16 2015-05-21 Terumo Bct, Inc. Expanding cells in a bioreactor
CN106377547B (zh) * 2016-09-30 2019-05-31 孔五一 脐带血再生粒子的提取方法及其用途
CN106635968A (zh) * 2016-10-14 2017-05-10 中卫华医（北京）生物科技有限公司 人脐带源间充质干细胞诱导分化成软骨细胞的方法
TWI656215B (zh) * 2017-11-28 2019-04-11 宏齊科技股份有限公司 自周邊血液製備間質幹細胞群的方法及其用途
US11285177B2 (en) 2018-01-03 2022-03-29 Globus Medical, Inc. Allografts containing viable cells and methods thereof
KR102315584B1 (ko) 2020-05-29 2021-10-21 단국대학교 천안캠퍼스 산학협력단 청각 내이 내 lgr5+ 청각 전구세포 분리방법
CN113249315A (zh) * 2021-05-31 2021-08-13 上海南滨江细胞生物科技有限公司 一种脐血含有间充质干细胞的培养方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5837539A (en) * 1990-11-16 1998-11-17 Osiris Therapeutics, Inc. Monoclonal antibodies for human mesenchymal stem cells
WO1997039104A1 (en) * 1996-04-17 1997-10-23 Osiris Therapeutics, Inc. Cryopreservation and extensive subculturing of human mesenchymal stem cells
EP0941027A4 (de) * 1996-11-15 2000-08-09 Osiris Therapeutics Inc MSC-Megakaryozytenvorläuferzusammensetzung und Verfahren zum Isolieren von mit isolierten Megakaryozyten assoziierten MSCS durch die Isolierung von Megakaryozyten
WO1999061587A1 (en) * 1998-05-29 1999-12-02 Osiris Therapeutics, Inc. Human cd45+ and/or fibroblast + mesenchymal stem cells
EP1099754A1 (de) * 1999-11-10 2001-05-16 Universiteit Leiden Mesenchym-Stammzellen und/oder Vorlaüferzellen, ihre Isolierung und ihre Verwendung
EP2295539A1 (de) * 2000-06-26 2011-03-16 Nc Medical Research Inc. Zellfraktion mit Zellen welche in der Lage sind sich in Nervenzellen zu differenzieren
US7560280B2 (en) * 2000-11-03 2009-07-14 Kourion Therapeutics Gmbh Human cord blood derived unrestricted somatic stem cells (USSC)
EP1416944B1 (de) * 2001-08-14 2005-12-14 Medipost, Co., Ltd. Zusammensetzung zur behandlung von schäden des gelenkknorpels

Also Published As

Publication number Publication date
ES2287447T3 (es) 2007-12-16
DE60314602T2 (de) 2008-02-28
JP2005517441A (ja) 2005-06-16
CN1630717A (zh) 2005-06-22
AU2003207399A1 (en) 2003-09-09
EP1483371B1 (de) 2007-06-27
DE60314602D1 (de) 2007-08-09
KR100489248B1 (ko) 2005-05-11
KR20030069115A (ko) 2003-08-25
CN1281739C (zh) 2006-10-25
EP1483371A4 (de) 2005-07-27
DK1483371T3 (da) 2007-10-29
WO2003070922A1 (en) 2003-08-28
EP1483371A1 (de) 2004-12-08
US20100184218A1 (en) 2010-07-22
US20050118714A1 (en) 2005-06-02

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