WO2000077236A3 - Methods for enhancing the production of cytokines in cell cultur e - Google Patents

Methods for enhancing the production of cytokines in cell cultur e Download PDF

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Publication number
WO2000077236A3
WO2000077236A3 PCT/US2000/040199 US0040199W WO0077236A3 WO 2000077236 A3 WO2000077236 A3 WO 2000077236A3 US 0040199 W US0040199 W US 0040199W WO 0077236 A3 WO0077236 A3 WO 0077236A3
Authority
WO
WIPO (PCT)
Prior art keywords
production
cytokines
enhancing
methods
cytokine
Prior art date
Application number
PCT/US2000/040199
Other languages
French (fr)
Other versions
WO2000077236A2 (en
Inventor
Allan S Lau
Laura Browning
Original Assignee
Genetrol Biotherapeutics
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetrol Biotherapeutics filed Critical Genetrol Biotherapeutics
Priority to JP2001503677A priority Critical patent/JP2003509012A/en
Priority to CA002376487A priority patent/CA2376487A1/en
Priority to EP00951042A priority patent/EP1255848A2/en
Priority to AU64037/00A priority patent/AU6403700A/en
Publication of WO2000077236A2 publication Critical patent/WO2000077236A2/en
Publication of WO2000077236A3 publication Critical patent/WO2000077236A3/en
Priority to HK03100710.6A priority patent/HK1048650A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention is directed to methods for enhancing the production of cytokines in mammalian cell culture by modifying the level of a cytokine regulatory factor in the cells, resulting in enhanced levels of cytokine production. The invention is further directed to cell lines which exhibit enhanced cytokine regulatory factor expression and enhanced cytokine expression.
PCT/US2000/040199 1999-06-15 2000-06-14 Methods for enhancing the production of cytokines in cell cultur e WO2000077236A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2001503677A JP2003509012A (en) 1999-06-15 2000-06-14 Methods for enhancing cytokine production in cell culture
CA002376487A CA2376487A1 (en) 1999-06-15 2000-06-14 Methods for enhancing the production of cytokines in cell culture
EP00951042A EP1255848A2 (en) 1999-06-15 2000-06-14 Methods for enhancing the production of cytokines in cell cultur e
AU64037/00A AU6403700A (en) 1999-06-15 2000-06-14 Methods for enhancing the production of cytokines in cell culture
HK03100710.6A HK1048650A1 (en) 1999-06-15 2003-01-28 Methods for enhancing the production of cytokines in cell cultur e

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13931399P 1999-06-15 1999-06-15
US60/139,313 1999-06-15

Publications (2)

Publication Number Publication Date
WO2000077236A2 WO2000077236A2 (en) 2000-12-21
WO2000077236A3 true WO2000077236A3 (en) 2002-09-12

Family

ID=22486056

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/040199 WO2000077236A2 (en) 1999-06-15 2000-06-14 Methods for enhancing the production of cytokines in cell cultur e

Country Status (7)

Country Link
EP (1) EP1255848A2 (en)
JP (1) JP2003509012A (en)
CN (1) CN1399681A (en)
AU (1) AU6403700A (en)
CA (1) CA2376487A1 (en)
HK (1) HK1048650A1 (en)
WO (1) WO2000077236A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010031859A1 (en) * 1999-09-08 2001-10-18 Lau Allan S. High level cytokine production with enhanced cell viability
JP2004529851A (en) * 2000-09-12 2004-09-30 ジーントロール バイオセラピューティクス， インコーポレイテッド Composition containing a mixture of human cytokines and method for producing the same
WO2002022786A1 (en) 2000-09-14 2002-03-21 Genetrol Biotherapeutics, Inc. Method and cell composition for screening compounds for anti-inflammatory activity
WO2002062837A2 (en) * 2000-12-19 2002-08-15 Genetrol Biotherapeutics, Inc. Cells with enhanced pkr expression for increasing cytokine production
US20090232808A1 (en) * 2005-01-28 2009-09-17 Apollo Life Sciences Limited Molecules and chimeric molecules thereof
WO2012048298A2 (en) 2010-10-08 2012-04-12 Caridianbct, Inc. Methods and systems of growing and harvesting cells in a hollow fiber bioreactor system with control conditions
EP3068867B1 (en) 2013-11-16 2018-04-18 Terumo BCT, Inc. Expanding cells in a bioreactor
WO2015148704A1 (en) 2014-03-25 2015-10-01 Terumo Bct, Inc. Passive replacement of media
CN106715676A (en) 2014-09-26 2017-05-24 泰尔茂比司特公司 Scheduled feed
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
EP3562936B1 (en) * 2017-01-02 2024-05-22 F. Hoffmann-La Roche AG B-cell cultivation method
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
WO2018184028A2 (en) 2017-03-31 2018-10-04 Terumo Bct, Inc. Cell expansion
JP2024511064A (en) 2021-03-23 2024-03-12 テルモ ビーシーティー、インコーポレーテッド Cell capture and proliferation

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993017043A1 (en) * 1992-02-28 1993-09-02 Brigham & Women's Hospital Methods and compounds for prevention of graft rejection
WO1997008324A1 (en) * 1995-08-22 1997-03-06 The Regents Of The University Of California Methods for enhancing the production of interferon in cell culture
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
WO1993017043A1 (en) * 1992-02-28 1993-09-02 Brigham & Women's Hospital Methods and compounds for prevention of graft rejection
WO1997008324A1 (en) * 1995-08-22 1997-03-06 The Regents Of The University Of California Methods for enhancing the production of interferon in cell culture

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BRETON J J ET AL: "THE NATURAL PRODUCT HYMENIALDISINE INHIBITS INTERLEUKIN-8 PRODUCTION IN U937 CELLS BY INHIBITION OF NUCLEAR FACTOR-KAPPA B", JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS,US,AMERICAN SOCIETY FOR PHARMACOLOGY AND, vol. 282, no. 1, 1997, pages 459 - 466, XP002910635, ISSN: 0022-3565 *
HARANT H ET AL: "1ALPHA,25-DIHYDROXYVITAMIN D3 AND A VARIETY OF ITS NATURAL METABOLITES TRANSCRIPTIONALLY REPRESS NUCLEAR-FACTOR-KB-MEDIATED INTERLEUKIN-8 GENE EXPRESSION", EUROPEAN JOURNAL OF BIOCHEMISTRY,DE,BERLIN, vol. 282, no. 1, November 1997 (1997-11-01), pages 63 - 71, XP002915093, ISSN: 0014-2956 *
KUMAR ASEEM ET AL: "Double-stranded RNA-dependent protein kinase activates transcription factor NF-kappa-B by phosphorylating I-kappa-B.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 91, no. 14, 1994, 1994, pages 6288 - 6292, XP002170325, ISSN: 0027-8424 *
LIBERMANN T A ET AL: "ACTIVATION OF INTERLEUKIN-6 GENE EXPRESSION THROUGH THE NF-KAPPA-B TRANSCRIPTION FACTOR", MOLECULAR AND CELLULAR BIOLOGY, vol. 10, no. 5, 1990, pages 2327 - 2334, XP002160675, ISSN: 0270-7306 *
LINDHOLM P F ET AL: "EXTRACELLULAR TAX-1 PROTEIN STIMULATES TUMOR NECROSIS FACTOR-BETA AND IMMUNOGLOBULIN KAPPA LIGHT CHAIN EXPRESSION IN LYMPHOID CELLS", JOURNAL OF VIROLOGY, vol. 66, no. 3, 1992, pages 1294 - 1302, XP000993234, ISSN: 0022-538X *

Also Published As

Publication number Publication date
WO2000077236A2 (en) 2000-12-21
JP2003509012A (en) 2003-03-11
CA2376487A1 (en) 2000-12-21
AU6403700A (en) 2001-01-02
CN1399681A (en) 2003-02-26
HK1048650A1 (en) 2003-04-11
EP1255848A2 (en) 2002-11-13

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