US20200330481A1 - Betulin-containing birch bark extracts and their formulation - Google Patents

Betulin-containing birch bark extracts and their formulation Download PDF

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US20200330481A1
US20200330481A1 US16/959,098 US201916959098A US2020330481A1 US 20200330481 A1 US20200330481 A1 US 20200330481A1 US 201916959098 A US201916959098 A US 201916959098A US 2020330481 A1 US2020330481 A1 US 2020330481A1
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oleogel
birch bark
emulsion
bark extract
carried out
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John Ashleigh Watson
Sebastian Jäger
Tobias Zahn
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Amryt Pharmaceuticals Designated Activity Co
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Amryt Research Ltd
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Publication of US20200330481A1 publication Critical patent/US20200330481A1/en
Assigned to ARES CAPITAL CORPORATION, AS ADMINISTRATIVE AGENT reassignment ARES CAPITAL CORPORATION, AS ADMINISTRATIVE AGENT SECURITY INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: AMRYT RESEARCH LIMITED
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F13/00—Bandages or dressings; Absorbent pads
    • A61F13/00051—Accessories for dressings
    • A61F13/00063—Accessories for dressings comprising medicaments or additives, e.g. odor control, PH control, debriding, antimicrobic
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A61K31/047—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates having two or more hydroxy groups, e.g. sorbitol
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    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
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    • A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
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    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A61L15/44—Medicaments
    • A—HUMAN NECESSITIES
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    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
    • A61L26/0061—Use of materials characterised by their function or physical properties
    • A61L26/0066—Medicaments; Biocides
    • A—HUMAN NECESSITIES
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    • A—HUMAN NECESSITIES
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    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera

Definitions

  • the present disclosure relates to pharmaceutical formulations derived from the extracts of birch bark.
  • the triterpenes found in birch bark extracts are known to have wound healing properties. Methods of extracting these triterpenes from birch bark are reported in U.S. Pat. No. 7,482,383. These methods provide solid birch bark extracts that may be used in pharmaceutical formulations. For example, emulsions containing such extracts are described in U.S. Pat. No. 7,482,383, and oleogels containing such extracts are described in U.S. Pat. Nos. 9,352,041; 8,828,444 and 8,536,380.
  • an oleogel For clinical use in wound healing, an oleogel must be applied by touch to the area of the skin where treatment is needed. Touch application is disadvantageous for the treatment of certain skins conditions (e.g., epidermolysis bullosa) because the simple act of applying the oleogel may lead to worsening of the skin condition.
  • Touch application is disadvantageous for the treatment of certain skins conditions (e.g., epidermolysis bullosa) because the simple act of applying the oleogel may lead to worsening of the skin condition.
  • the present disclosure provides clinically-advantageous wound-healing formulations (including oleogels, emulsions and foams) with improved rheological properties which overcome the disadvantages of the known solid birch bark-containing emulsions and oleogels.
  • the present disclosure provides a solid birch bark extract that contains at least about 70% by weight of betulin and one or more triterpenes selected from the group consisting of betulinic acid, oleanolic acid, erythrodiol and lupeol.
  • the solid birch bark extracts described herein may be formulated in an oleogel that possesses clinically-advantageous rheological properties.
  • the present disclosure also provides a method of making a solid birch bark extract that may be dispersed in a nonpolar solvent to provide a clinically-advantageous oleogel comprising the steps of (a) contacting birch bark with a suitable solvent to form an extraction solution containing betulin and at least one triterpene; (b) separating the birch bark from the extraction solution; (c) cooling the extraction solution to crystallize a portion of the betulin and triterpene from the solution; (d) separating the crystallized betulin and triterpene; (e) drying the separated, crystallized betulin and triterpene to form a solid birch bark extract.
  • the present disclosure also provides the solid birch bark extracts that are prepared according to these methods.
  • the present disclosure provides a clinically-advantageous oleogel comprising about 1% to about 20% by weight of particles of the solid birch bark extract dispersed in about 80% to about 99% of one or more nonpolar liquids the solid birch bark extracts are dispersed in a suitable nonpolar liquid to form an oleogel.
  • a sterile wound dressing comprising a pad and a therapeutically effective amount of the oleogel is also provided.
  • the present disclosure provides an emulsion comprising an oleogel of the present invention and further provides a foam comprising an emulsion of the present disclosure.
  • the present disclosure also provides methods of treating a wound in a patient by topically administering an effective amount of an oleogel, an emulsion or a foam of the present disclosure to at least a portion of the wound.
  • the present disclosure also provides methods of treating epidermolysis bullosa in a patient in need thereof comprising topically administering an effective amount of an oleogel, an emulsion or a foam of the present disclosure to an area of epidermolysis bullosa of the patient.
  • the present disclosure provides a solid birch bark extract, oleogel, sterile wound dressing, process of preparation, emulsion, foam, pressurized container, method of treating a wound, and method of treating epidermolysis bullosa according to the following numbered embodiments:
  • Time H 3 PO 4 Flow rate (min) Acetonitrile Water (v/v) (ml/min) 0 30 70 0.1% 0.6 5 30 30 0.1% 0.6 15 98 2 0.1% 0.6 20 98 2 0.1% 0.6 21 30 70 0.1% 0.6 30 30 70 0.1% 0.6
  • FIG. 1 shows the HPLC chromatograph taken from a solid birch bark extract monitoring 210 nm (top figure) and 320 nm (bottom figure).
  • the x-axis in FIG. 1 is the retention time (min), and the y-axis is the peak intensity (mAU).
  • FIG. 2 illustrates the continuous extraction of birch bark to provide an extraction solution comprising betulin and one or more triterpenes.
  • administering includes any mode of administration, such as oral, subcutaneous, sublingual, transmucosal, parenteral, intravenous, intra-arterial, buccal, sublingual, topical, vaginal, rectal, ophthalmic, otic, nasal, inhaled, and transdermal.
  • administering can also include prescribing or filling a prescription for a dosage form comprising a particular compound.
  • administering can also include providing directions to carry out a method involving a particular compound or a dosage form comprising the compound.
  • birch bark means the cortex of white-barked birch trees. Preferred embodiments include birch bark derived from Betula pendula Roth and Betula pubescens Ehrh as well as hybrids of both species.
  • RRT relative retention time
  • RT unkown is the retention time of the unknown peak and RT betulin is the retention time of betulin peak (210 nm) measured by the following HPLC method:
  • Time H 3 PO 4 Flow rate (min) Acetonitrile Water (v/v) (ml/min) 0 30 70 0.1% 0.6 5 30 30 0.1% 0.6 15 98 2 0.1% 0.6 20 98 2 0.1% 0.6 21 30 70 0.1% 0.6 30 30 70 0.1% 0.6
  • substantially identical means an analytical spectrum, such as HPLC chromatogram, NMR spectrum, and etc., which resembles the reference spectrum to a great degree in both the peak locations and their intensity.
  • a HPLC chromatogram is “substantially identical” to a reference chromatogram if the peak locations (relative retention times) in the HPLC chromatogram vary by no more than ⁇ 5% from the peak locations (relative retention times) in the reference chromatogram.
  • the relative peak intensities in the HPLC chromatogram may vary by no more than ⁇ 10% from the peak intensities in the reference chromatogram.
  • “Therapeutically effective amount” means the amount of an active substance that, when administered to a subject for treating a disease, disorder, or other undesirable medical condition, is sufficient to have a beneficial effect with respect to that disease, disorder, or condition.
  • the therapeutically effective amount will vary depending on the chemical identity and formulation form of the active substance, the disease or condition and its severity, and the age, weight, and other relevant characteristics of the patient to be treated. Determining the therapeutically effective amount of a given active substance is within the ordinary skill of the art and typically requires no more than routine experimentation.
  • substantially free means that the ingredient indicated is not present, or is present in only insignificant amounts. In one embodiment, “substantially free” means less than about 10% (e.g. less than about 10 wt. %). In other embodiments, “substantially free” means less than about 5% (e.g. less than about 5 wt. %), less than about 2% (e.g. less than about 2 wt. %), or less than about 1% (e.g. less than about 1 wt. %), or about 0% (e.g. about 0 wt. %). For example, an emulsion that is substantially free of an emulsifier does not contain any emulsifier in a substantial amount (e.g.
  • an oleogel that is substantially free of solid birch bark extract particles having a size greater than about 50 ⁇ m does not contain any solid birch bark extract particles having a size greater than about 50 ⁇ m in a substantial amount (e.g. contains less than about 10 wt. %, less than about 5 wt. %, less than about 2 wt. %, or less than about 1 wt. % of solid birch bark extract particles having a size greater than about 50 ⁇ m; or contains 0 wt. % of solid birch bark extract particles having a size greater than about 50 ⁇ m).
  • the present disclosure provides solid birch bark extracts that may be formulated in clinically-advantageous oleogels. Without being bound by any theory, it is believed that the chemical composition and the particle morphology of the solid birch bark extracts produced according to the methods of the present disclosure are responsible these advantages.
  • the solid birch bark extracts of the present disclosure may be characterized on the basis of their chemical composition.
  • the solid birch bark extracts of the present disclosure comprise lupane and oleanane triterpenes.
  • the birch bark extracts may contain the lupane triterpenes: betulin, lupeol, and betulinic acid, and the oleanane triterpenes: erythrodiol and oleanolic acid.
  • the presence of a particular triterpene in the birch extract may be determined using relative retention times obtained from HPLC chromatography.
  • HPLC method is used to determine the chemical composition of the solid birch bark extracts of the present disclosure:
  • Time H 3 PO 4 Flow rate (min) Acetonitrile Water (v/v) (ml/min) 0 30 70 0.1% 0.6 5 30 30 0.1% 0.6 15 98 2 0.1% 0.6 20 98 2 0.1% 0.6 21 30 70 0.1% 0.6 30 30 70 0.1% 0.6
  • a Phenomenex Kinetix C18 column is exemplary of an HPLC column having a C-18 modified silica stationary phase on core-shell silica solid support (2.6 ⁇ m HILIC 100 ⁇ ) with the above noted column dimensions.
  • betulinic acid has a relative retention time of about 0.75-0.90
  • oleanolic acid has a relative retention time of about 0.84-0.97
  • betulin has a relative retention time of 1.00
  • erythrodiol has a relative retention time of about 1.25-1.40
  • lupeol has a relative retention time of about 3.50-4.15.
  • the solid birch bark extract has an HPLC chromatogram substantially identical to FIG. 1 .
  • the solid birch bark extract comprises at least about 50 wt. %, at least about 55 wt. %, at least about 60 wt. %, at least about 65 wt. %, at least about 70 wt. %, at least about 75 wt. %, at least about 80 wt. %, at least about 85 wt. %, or at least about 90% by weight betulin and one or more triterpenes.
  • the one or more triterpenes is selected from the group consisting of betulinic acid, oleanolic acid, erythrodiol and lupeol.
  • the solid birch bark extract comprises at least one of the following substances: 3- ⁇ -caffeoyl betulin, acetate of the methylester of betulinic acid, acetyloleanolic acid, allobetulin, betulinic aldehyde, betulonic acid, betulonic aldehyde, lupane-3 ⁇ ,20,28-triol, lupane-3 ⁇ ,20-diol (monogynol), oleanolic aldehyde, sitosterol, ursolic acid, or ⁇ -amyrin
  • the solid birch bark extract of the present disclosure may be characterized by the particle size of the particles of the solid birch bark extract.
  • the average particle size of the particles of the solid birch bark extract is less than about 100 ⁇ m, less than about 90 ⁇ m, less than about 80 ⁇ m, less than about 70 ⁇ m, less than about 60 ⁇ m, less than about 50 ⁇ m, less than about 40 ⁇ m, less than about 30 ⁇ m or less than about 25 ⁇ m.
  • the solid birch bark extract of the present disclosure is substantially free of solid birch bark extract particles having a particle size greater than about 30 ⁇ m, greater than about 40 ⁇ m, greater than about 50 ⁇ m, greater than about 60 ⁇ m, greater than about 70 ⁇ m, greater than about 80 ⁇ m, greater than about 90 ⁇ m or greater than about 100 ⁇ m.
  • the solid birch bark extracts are derived from Betula pendula Roth and Betula pubescens Ehrh as well as hybrids of both species.
  • the present disclosure provides methods for preparing solid birch bark extracts that may be formulated in clinically-advantageous oleogels.
  • the methods include the steps of obtaining birch trees, stripping and processing the bark from said birch trees, contacting the processed birch bark with a suitable solvent to provide an extraction solution comprising betulin and one or more triterpenes, and isolating and drying the birch bark extract comprising betulin and one or more triterpenes from the extraction solution.
  • the isolated the birch bark extract is in the form of a solid.
  • the method comprises: (a) contacting birch bark with a pharmaceutically acceptable solvent to form a extraction solution comprising betulin and one or more triterpenes; (b) separating the birch bark from the extraction solution; (c) cooling the extraction solution whereby a portion of the betulin and one or more triterpenes crystallizes from the cooled extraction solution; (d) separating the crystallized betulin and one or more triterpenes from the cooled extraction solution; and (e) drying the separated, crystallized betulin and one or more triterpenes to provide the solid birch bark extract.
  • the birch bark used in step (a) is first processed to improve the extraction efficiency of betulin and one or more triterpenes from the birch bark. For example, experiments have shown that the yield of the dry extract from birch bark is highest when the extracted birch bark particles are small.
  • the particle size of the birch bark is reduced prior to step (a) using a mill, or other suitable equipment, until the resulting birch bark passes through a sieve with a mesh size of about 1.25 mm.
  • the birch bark is contacted with a pharmaceutically acceptable solvent at a temperature of between about 50° C. and about 200° C.
  • the temperature is between about 60° C. and about 100° C., between about 60° C. and about 110° C., between about 60° C. to about 120° C., between about 60° C. to about 130° C., between about 70° C. to about 130° C., between about 80° C. to about 130° C., between about 90° C. to about 130° C., or between about 100° C. and 150° C.
  • the temperature is between about 115° C. and 130° C.
  • the birch bark is contacted with a pharmaceutically acceptable solvent at a pressure between about 2 to 10 bars. In one embodiment, the pressure is about 4.5 bars.
  • solvents are known to those of skill in the art and include hydrocarbons, alcohols, ketones, ethers esters, sulfoxides, etc.
  • examples of pharmaceutically acceptable solvents include, without limitation, 1-butanol, 1-pentanol, 1-propanol, 2-butanol, 2-methyl-1-propanol, 2-propanol, 3-methyl-1-butanol, acetic acid, acetone, anisole, butyl acetate, dimethyl sulfoxide, ethanol, ethyl acetate, ethyl ether, ethyl formate, formic acid, heptane, hexane, isobutyl acetate, isopropyl acetate, methyl acetate, methylethyl ketone, methylisobutyl ketone, pentane, propyl acetate, tert-butylmethyl ether, diisopropyl ether, methyl tert-
  • two or more pharmaceutically acceptable solvents are mixed to provide the pharmaceutically acceptable solvent.
  • the birch bark is contacted with n-heptane at a temperature of between about 60° C. and 130° C. for between 8 to 12 minutes.
  • the (a) contacting birch bark with a pharmaceutically acceptable solvent to form an extraction solution comprising betulin and one or more triterpenes and (b) separating the birch bark from the extraction solution are run using a continuous extraction process (Shown in FIG. 2 ).
  • birch bark is mixed with n-heptane in a proportion of about 1:14 to 1:16 (w/v).
  • the mixture is extracted continuously using n-heptane as extraction solvent at a temperature of about 115-130° C. and at a pressure of about 4.5 bar (Nitrogen).
  • n-heptane as extraction solvent
  • the extracted cork is rinsed down by cold n-heptane, and at the upper end of the sedimentation vessel the extract (hot solution of triterpenes in n-heptane) is filtrated (10 ⁇ m filter) and transported through a second filter (1 ⁇ m filter) to the crystallisation step.
  • the cooling of step (c) is carried out at a temperature of about ⁇ 20° C. to about 35° C., about ⁇ 15° C. to about 35° C., ⁇ 10° C. to about 35° C., about ⁇ 5° C. to about 35° C., or about 0° C. to about 35° C.
  • the cooled extraction solution in step (c) is supersaturated at least about 1-fold, about 2-fold, about 3-fold, about 4-fold, about 5-fold or about 6-fold.
  • the cooled extraction solution is supersaturated about 2-fold or about 5-fold.
  • the drying step (e) is carried out under vacuum at a temperature of at least 50° C., at least 60° C., at least 70° C., at least 80° C. or at least 85° C. In certain embodiments, the drying step (e) is carried out at a temperature of about 65° C. to about 75° C., about 70° C. to about 80° C., about 75° C. to about 85° C., or about 85° C. to about 95° C.
  • drying step (e) is carried out under vacuum at a pressure of less than about 90 mbar, less than about 80 mbar, less than about 70 mbar, less than about 60 mbar or less than about 50 mbar. In certain embodiments, drying step (e) reduces the amount of the pharmaceutically acceptable solvent in the dry birch bark extract to less than about 0.5% (weight/weight).
  • the present invention also provides the solid birch bark extracts prepared according to the processes set forth above.
  • compositions Comprising Solid Birch Bark Extracts
  • the present disclosure provides clinically-advantageous wound-healing formulations comprising solid birch bark extracts.
  • the compositions include oleogels, emulsions, foams, and oleogel-impregnated sterile wound dressings.
  • the compositions are useful as topical wound healing agents.
  • Gels are finely dispersed systems comprising a liquid phase and a solid phase.
  • the solid phase forms a coherent three-dimensional framework and the two phases permeate one another.
  • Oleogels are hydrophobic gels based on a nonpolar liquid (for example, an oil, a wax, or a paraffin) to which a gel-forming agent is added to achieve the desired physical properties.
  • the present disclosure provides oleogels comprising a nonpolar liquid and an oleogel-forming agent.
  • Suitable nonpolar liquids for use in oleogels of the present disclosure include, for example, plant, animal, or synthetic oils, waxes, and paraffins.
  • the nonpolar liquid is a vegetable oil selected from the group consisting of: castor oil, peanut oil, jojoba oil, sunflower oil, olive oil, avocado oil, and almond oil.
  • the nonpolar liquid is sunflower oil.
  • the nonpolar liquid comprises at least one triglyceride. In certain embodiments, the at least one triglyceride is Miglyol. In other embodiments, the nonpolar liquid comprises at least one C7 or greater hydrocarbon. In certain embodiments, the at least one C7 or greater hydrocarbon is a paraffin.
  • the nonpolar liquid used in the oleogel has a peroxide value of lest than about 15, less than about 10, less than about 5, less than about 4, less than about, 3 or less than about 2. In certain embodiments, the nonpolar liquid has a peroxide value of no more than about 3.
  • peroxide value as used herein means the peroxide value is determined according to Ph. Eur. 2.5.5.
  • the present disclosure provides methods of making oleogels.
  • about 1 wt % to about 20 wt % of the dried solid birch bark extract is dispersed in nonpolar liquid to form an oleogel.
  • the nonpolar liquid is sunflower oil.
  • the oleogel is sterile.
  • the oleogel may be sterilized by suitable methods known to those skilled in the art, for example using ionizing radiation such as electron beam (EB), X-ray, or gamma.
  • EB electron beam
  • X-ray X-ray
  • gamma gamma
  • the oleogel is sterilized by ionizing (e.g., gamma) irradiation at doses less than about 40 kGy, less than about 35 kGy, less than about 30 kGy, less than about 25 kGy, less than about 20 kGy, or less than about 15 kGY.
  • the oleogel is sterilized by ionizing (e.g., gamma) irradiation at doses ranging from about 5 to about 25 kGy, about 9 to about 25 kGy, about 10 to about 25 kGy, about 11 to about 25 kGy, about 12 to about 25 kGy, or from about 11 to about 20 kGy.
  • ionizing e.g., gamma
  • the oleogel comprises between about 1 wt. % and about 30 wt. % solid birch bark extract dispersed in about 70 wt. % to about 99 wt. % of one or more nonpolar liquids, wherein the oleogel contains at least one oleogel forming agent in addition to the solid birch bark extract particles.
  • the oleogel comprises between about 1 wt. % and about 20 wt. % solid birch bark extract dispersed in about 80 wt. % to about 99 wt. % of one or more nonpolar liquids, wherein the oleogel contains at least one oleogel forming agent in addition to the solid birch bark extract particles.
  • the oleogel comprises between about 1 wt. % and about 30 wt. % solid birch bark extract particle dispersed in about 70 wt. % to about 99 wt. % of one or more nonpolar liquids, wherein the dispersed solid birch bark extract particles are the only oleogel forming agent in the oleogel.
  • the oleogel comprises between about 1 wt. % and about 20 wt. % solid birch bark extract dispersed in about 80 wt. % to about 99 wt. % of one or more nonpolar liquids, wherein the oleogel contains at least one oleogel forming agent in addition to the solid birch bark extract particles.
  • the oleogel comprises: about 5 wt. % solid birch bark extract particle dispersed in about 95 wt. % of one or more nonpolar liquids; about 10 wt. % solid birch bark extract particle dispersed in about 90 wt. % of one or more nonpolar liquids; about 15 wt. % solid birch bark extract particle dispersed in about 85 wt. % of one or more nonpolar liquids; or about 20 wt. % solid birch bark extract particle dispersed in about 80 wt. % of one or more nonpolar liquids.
  • the amount of solid birch bark extract particle (for example, about 1 wt. % and about 20 wt. %) includes up to about 0.5 wt % of solid birch bark extract particles that are dissolved in the nonpolar liquid.
  • the segregation of the nonpolar liquid from the oleogel is less than about 10%, about 9%, about 8%, about 7%, about 6%, about 5%, about 4%, about 3%, about 2%, about 1%, about 0.5%, or about 0.1% after centrifuging at 25° C. for about 30 minutes at 2750 g (e.g., 4,400 rpm).
  • the oleogels of the present disclosure may be characterized by their viscosity.
  • the viscosity at 200/s of the oleogel ranges from about 0.5 to about 4.0 Pa ⁇ s and the thixotropy value of the oleogel ranges from about 200 to about 1200 Pa ⁇ s as measured according to the rotating viscometer method described in Ph. Eur. 2.2.10 using a cone-plate viscometer.
  • the oleogels of the present disclosure may be also characterized by their consistency values as determined using a texture analyzer.
  • Texture analysis is primarily concerned with measurement of the mechanical properties of a product calculated from the results of a two cycle texture profile analysis test. Texture analysers perform this test by applying controlled forces to the product and recording its response in the form of force, deformation and time.
  • the speed of penetration is 0.4 mm/s.
  • the oleogels of the present disclosure may be distinguished from simple thickened mixtures of the solid birch bark extracts on the basis of consistency values. Specifically, the oleogels of the present disclosure may be characterized by having a consistency value of less than about 3000 mN, and at least about 250 mN as measured by a texture analyzer. In some embodiments, the consistency value of the oleogels is between about 300-2000 mN as measured by a texture analyser.
  • the present disclosure also provides methods for preparing oleogels.
  • the method comprises dispersing solid birch bark extract in a suitable nonpolar liquid.
  • a sterile wound dressing comprising a pad and a therapeutically effective layer comprising the oleogel of the present disclosure.
  • the pad is an adsorbent pad.
  • the pad is any solid material suitable for covering a wound, including cotton gauze.
  • the therapeutically effective layer of oleogel is applied to one or more surfaces of the pad (e.g., the surface of the pad intended to contact the skin or wound of the patient directly).
  • the pad is immersed in the oleogel such that the oleogel is disposed on at least a portion of the outer surfaces of the pad, and optionally in at least portions of the interior of the pad.
  • the pad can comprise a material suitable for dressing a wound that dissolve when used, releasing the oleogel.
  • materials can include absorbable materials comprising collagen, alginate, etc.
  • emulsion relates to heterogeneous systems consisting of two liquids that are not miscible with each other or only miscible to a limited extent, which are typically designated as phases.
  • one of the two liquids is dispersed in the other liquid in the form of minute droplets.
  • an emulsion comprising the solid birch bark extract of the present disclosure is provided.
  • Other embodiments provide emulsions comprising the oleogels of the present disclosure.
  • the emulsions of the present disclosure include an emulsifier.
  • the emulsifier is (hydropropyl)methyl cellulose.
  • the emulsions are substantially free of an emulsifier.
  • foams may be superior to oleogels because foams can be applied to wounds almost touchless whereas the application of an oleogel requires touch.
  • Foams are generally based on emulsions where a propellant is mixed with the dispersed lipid phase of an emulsion.
  • the present disclosure provides foams comprising a solid birch bark extract-containing emulsion as described above.
  • the foam comprises an oleogel consisting of about 5 wt. % to about 10 wt. % solid birch bark extract and the emulsion is a water-in-oil emulsion consisting of the oleogel and about 20 wt. % to about 30 wt. % of water.
  • the foam comprises an oleogel consisting of about 7 wt. % solid birch bark extract and the emulsion is a water-in-oil emulsion consisting of the oleogel and about 25 wt. % of water.
  • the foams of the present disclosure further comprise an emulsifier.
  • Emulsifiers are well known in the art as substances that stabilize emulsions, and include surfactants.
  • Emulsifiers useful in the present invention are those that are acceptable for pharmaceutical use, particularly for skin or wound contact.
  • suitable emulsifiers include emulsifying waxes, cetearyl alcohol, polysorbate 20, ceteareth 20, etc.
  • the emulsifier is selected from the group consisting of phosphatidyl choline, polyglyceryl-3-methyl glucose distearate, and combinations thereof.
  • the oleogels, emulsions, and foams of the present disclosure further comprise a disinfectant.
  • the disinfectant is selected from the group consisting of ethanol, propan-1-ol (n-propanol), and propan-2-ol (isopropanol).
  • the oleogels, emulsions, and foams of the present disclosure further comprise an antibiotic, particularly lipophilic antibiotics such as fluoroquinolones, macrolides, tigecycline, lincosamides, rifampin, linezolid, tetracyclines, and chloramphenicol.
  • the foams of the present disclosure possess certain physical properties.
  • the foam index is greater than about 2.
  • the emulsion used in the foam exhibits an interfacial surface tension of greater than about 4 nM/m using methods known in the art.
  • the term “foam index” as used herein is the ratio of the foamed and unfoamed densities of the specified material.
  • the foam index of a particular foam is the ratio of the density of the foamed material to the density of the material prior to foaming.
  • the present disclosure also provides for pressurized containers filled with an emulsion of the present invention and a pharmaceutically acceptable propellant whereby the emulsion forms a foam upon decanting at least a portion of the mixture from the container.
  • the present disclosure also provides methods of treating a wound in a patient by topically administering an effective amount of an oleogel, an emulsion or a foam of the present disclosure to at least a portion of the wound.
  • the wound treated is selected from the group consisting of burns (such as mild to severe burns), surgical skin lesions, superficial injuries, chronic wounds (such as pressure ulcers, diabetic foot ulcers, chronic venous ulcers, artery insufficiency ulcers, etc.), aesthetic skin treatments (such as ablative laser skin treatments, chemical peels, dermabrasion, etc.), wounds resulting from adverse drug reactions (such as toxic epidermal necrolysis, Lyell syndrome, Stevens-Johnson syndrome, radiation-induced dermatitis, chemotherapy-induced dermatitis, etc.), rare skin diseases (such as epidermolysis bullosa, pemphigus vulgaris, pemphigoid, bullous pemphigoid, pemphigus foliaceus, pyoderma gangrinosa, etc.) and combinations thereof.
  • burns such as mild to severe burns
  • surgical skin lesions such as superficial injuries, chronic wounds (such as pressure ulcers, diabetic foot ulcers, chronic venous ulcers
  • the present disclosure also provides methods of treating epidermolysis bullosa in a patient in need thereof comprising topically administering an effective amount of an oleogel, an emulsion or a foam of the present disclosure to an area of epidermolysis bullosa of the patient.

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11083733B2 (en) 2018-01-04 2021-08-10 Amryt Research Limited Betulin-containing birch bark extracts and their formulation
US11266660B2 (en) 2018-01-04 2022-03-08 Amryt Research Limited Betulin-containing birch bark extracts and their formulation
US11826374B2 (en) 2018-01-04 2023-11-28 Amryt Research Limited Betulin-containing birch bark extracts and their formulation
US12268695B2 (en) 2018-01-04 2025-04-08 Amryt Pharmaceuticals Designated Activity Company Betulin-containing birch bark extracts and their formulation

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