US20130089928A1 - Serum-Free Chemically Defined Cell Culture Medium - Google Patents
Serum-Free Chemically Defined Cell Culture Medium Download PDFInfo
- Publication number
- US20130089928A1 US20130089928A1 US13/703,296 US201113703296A US2013089928A1 US 20130089928 A1 US20130089928 A1 US 20130089928A1 US 201113703296 A US201113703296 A US 201113703296A US 2013089928 A1 US2013089928 A1 US 2013089928A1
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- serum
- cell culture
- culture medium
- chemically defined
- defined cell
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- A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
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- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
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- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
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- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0655—Chondrocytes; Cartilage
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- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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- C12N2500/38—Vitamins
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/135—Platelet-derived growth factor [PDGF]
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
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Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13/703,296 US20130089928A1 (en) | 2010-06-17 | 2011-06-16 | Serum-Free Chemically Defined Cell Culture Medium |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US39784710P | 2010-06-17 | 2010-06-17 | |
US13/703,296 US20130089928A1 (en) | 2010-06-17 | 2011-06-16 | Serum-Free Chemically Defined Cell Culture Medium |
PCT/US2011/001088 WO2011159359A2 (en) | 2010-06-17 | 2011-06-16 | Serum-free chemically defined cell culture medium |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2011/001088 A-371-Of-International WO2011159359A2 (en) | 2010-06-17 | 2011-06-16 | Serum-free chemically defined cell culture medium |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US15/166,965 Continuation US10287550B2 (en) | 2010-06-17 | 2016-05-27 | Serum-free chemically defined cell culture medium |
Publications (1)
Publication Number | Publication Date |
---|---|
US20130089928A1 true US20130089928A1 (en) | 2013-04-11 |
Family
ID=45348785
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US13/703,296 Abandoned US20130089928A1 (en) | 2010-06-17 | 2011-06-16 | Serum-Free Chemically Defined Cell Culture Medium |
US15/166,965 Expired - Fee Related US10287550B2 (en) | 2010-06-17 | 2016-05-27 | Serum-free chemically defined cell culture medium |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US15/166,965 Expired - Fee Related US10287550B2 (en) | 2010-06-17 | 2016-05-27 | Serum-free chemically defined cell culture medium |
Country Status (9)
Country | Link |
---|---|
US (2) | US20130089928A1 (zh) |
EP (1) | EP2582788A4 (zh) |
JP (1) | JP2013529917A (zh) |
KR (1) | KR20130112028A (zh) |
CN (1) | CN103068969A (zh) |
AU (1) | AU2011265723A1 (zh) |
BR (1) | BR112012032164A2 (zh) |
MX (1) | MX2012014232A (zh) |
WO (1) | WO2011159359A2 (zh) |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2016179243A1 (en) * | 2015-05-05 | 2016-11-10 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Reversion of primed pluripotent stem cells to naive pluripotent stem cells |
USD848022S1 (en) | 2017-07-14 | 2019-05-07 | Hope Biosciences, Llc | Container for storing biological material |
USD875967S1 (en) | 2017-07-14 | 2020-02-18 | Hope Biosciences, Llc | Container for storing biological material |
US10894947B1 (en) | 2016-04-29 | 2021-01-19 | Hope Biosciences, Llc | Method for generating protein rich conditioned medium |
US10959425B2 (en) | 2016-04-29 | 2021-03-30 | Hope Biosciences, Llc | Method of banking stem cells |
US10988731B2 (en) | 2016-04-29 | 2021-04-27 | Hope Biosciences, Llc | Formulation for storage, transportation, and delivery of protein rich conditioned medium |
US11111480B2 (en) | 2016-04-29 | 2021-09-07 | Hope Biosctences, Llc | Culture media for multipotent stem cells |
CN113512533A (zh) * | 2021-07-30 | 2021-10-19 | 创芯国际生物科技(广州)有限公司 | 一种高精密组织芯片的制备方法及应用 |
US11396540B1 (en) * | 2014-09-05 | 2022-07-26 | Coherus Biosciences, Inc. | Methods for making recombinant proteins |
WO2023148556A1 (en) * | 2022-02-07 | 2023-08-10 | Smartcella Solutions Ab | Methods and compositions for generating human induced mesenchymal stem cells |
Families Citing this family (32)
Publication number | Priority date | Publication date | Assignee | Title |
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JP5993113B2 (ja) * | 2011-01-31 | 2016-09-14 | 株式会社 バイオミメティクスシンパシーズ | アルツハイマー病治療に向けたヒト脂肪組織由来間葉系幹細胞 |
US20140328808A1 (en) * | 2011-10-27 | 2014-11-06 | National University Corporation Tokyo Medical And Dental University | Technique for isolating/culturing colorectal epithelial stem cell, and technique for transplanting colorectal epithelium employing said technique |
WO2013165252A1 (en) * | 2012-05-03 | 2013-11-07 | Erasmus University Medical Center Rotterdam | Culturing of mesenchymal stem cells |
KR101523040B1 (ko) * | 2012-06-26 | 2015-05-26 | 라정찬 | 고농도의 줄기세포 제조방법 |
CN103060264B (zh) * | 2012-12-20 | 2015-01-21 | 上海市第十人民医院 | 一种干细胞培养基及其应用和干细胞培养方法 |
CN103255103B (zh) * | 2013-04-17 | 2015-01-14 | 冯文峰 | 一种无血清的脂肪间充质干细胞培养基 |
JP6502255B2 (ja) * | 2013-06-12 | 2019-04-17 | 株式会社 資生堂 | Pdgfを含有するds細胞用無血清培地 |
NL2011170C2 (en) * | 2013-07-15 | 2015-01-21 | Univ Erasmus Medical Ct | Method and culture medium for in vitro culturing of stem cells. |
KR102219743B1 (ko) * | 2013-11-01 | 2021-02-23 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | 신규 연골 세포 유도 방법 |
CN104630138B (zh) * | 2013-11-06 | 2017-11-14 | 陕西瑞盛生物科技有限公司 | 一种无血清软骨细胞培养液 |
CN103805562B (zh) * | 2014-01-13 | 2015-08-05 | 章毅 | 培养胎盘间充质干细胞的无血清培养基 |
CN113957043A (zh) * | 2015-03-04 | 2022-01-21 | 迈索布拉斯特国际有限公司 | 间充质干细胞的细胞培养方法 |
FR3035407B1 (fr) | 2015-04-23 | 2022-06-17 | Francais Du Sang Ets | Procede de conservation de cellules, tissus ou organes en hypothermie |
CN104830753B (zh) * | 2015-05-20 | 2018-07-10 | 广州赛莱拉干细胞科技股份有限公司 | 一种诱导多能干细胞培养基、应用及培养方法 |
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US10287550B2 (en) | 2019-05-14 |
WO2011159359A2 (en) | 2011-12-22 |
WO2011159359A3 (en) | 2012-02-23 |
MX2012014232A (es) | 2013-03-21 |
KR20130112028A (ko) | 2013-10-11 |
US20160281060A1 (en) | 2016-09-29 |
EP2582788A2 (en) | 2013-04-24 |
CN103068969A (zh) | 2013-04-24 |
WO2011159359A4 (en) | 2012-04-19 |
JP2013529917A (ja) | 2013-07-25 |
EP2582788A4 (en) | 2014-03-19 |
AU2011265723A1 (en) | 2013-01-10 |
BR112012032164A2 (pt) | 2017-11-28 |
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