BR112012032164A2 - meio de cultura celular definido quimicamente e isento de soro - Google Patents
meio de cultura celular definido quimicamente e isento de soroInfo
- Publication number
- BR112012032164A2 BR112012032164A2 BR112012032164A BR112012032164A BR112012032164A2 BR 112012032164 A2 BR112012032164 A2 BR 112012032164A2 BR 112012032164 A BR112012032164 A BR 112012032164A BR 112012032164 A BR112012032164 A BR 112012032164A BR 112012032164 A2 BR112012032164 A2 BR 112012032164A2
- Authority
- BR
- Brazil
- Prior art keywords
- serum
- culture medium
- cell culture
- chemically defined
- defined cell
- Prior art date
Links
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0655—Chondrocytes; Cartilage
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/36—Lipids
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/105—Insulin-like growth factors [IGF]
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/135—Platelet-derived growth factor [PDGF]
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/33—Insulin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
- C12N2501/415—Wnt; Frizzeled
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/80—Neurotransmitters; Neurohormones
- C12N2501/825—Serotonine (5-HT); Melatonine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US39784710P | 2010-06-17 | 2010-06-17 | |
PCT/US2011/001088 WO2011159359A2 (en) | 2010-06-17 | 2011-06-16 | Serum-free chemically defined cell culture medium |
Publications (1)
Publication Number | Publication Date |
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BR112012032164A2 true BR112012032164A2 (pt) | 2017-11-28 |
Family
ID=45348785
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112012032164A BR112012032164A2 (pt) | 2010-06-17 | 2011-06-16 | meio de cultura celular definido quimicamente e isento de soro |
Country Status (9)
Country | Link |
---|---|
US (2) | US20130089928A1 (pt) |
EP (1) | EP2582788A4 (pt) |
JP (1) | JP2013529917A (pt) |
KR (1) | KR20130112028A (pt) |
CN (1) | CN103068969A (pt) |
AU (1) | AU2011265723A1 (pt) |
BR (1) | BR112012032164A2 (pt) |
MX (1) | MX2012014232A (pt) |
WO (1) | WO2011159359A2 (pt) |
Families Citing this family (42)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5993113B2 (ja) * | 2011-01-31 | 2016-09-14 | 株式会社 バイオミメティクスシンパシーズ | アルツハイマー病治療に向けたヒト脂肪組織由来間葉系幹細胞 |
US20140328808A1 (en) * | 2011-10-27 | 2014-11-06 | National University Corporation Tokyo Medical And Dental University | Technique for isolating/culturing colorectal epithelial stem cell, and technique for transplanting colorectal epithelium employing said technique |
WO2013165252A1 (en) * | 2012-05-03 | 2013-11-07 | Erasmus University Medical Center Rotterdam | Culturing of mesenchymal stem cells |
KR101523040B1 (ko) * | 2012-06-26 | 2015-05-26 | 라정찬 | 고농도의 줄기세포 제조방법 |
CN103060264B (zh) * | 2012-12-20 | 2015-01-21 | 上海市第十人民医院 | 一种干细胞培养基及其应用和干细胞培养方法 |
CN103255103B (zh) * | 2013-04-17 | 2015-01-14 | 冯文峰 | 一种无血清的脂肪间充质干细胞培养基 |
CA2917684C (en) * | 2013-06-12 | 2021-07-20 | Shiseido Company, Ltd. | Serum-free medium containing pdgf for ds cells |
NL2011170C2 (en) * | 2013-07-15 | 2015-01-21 | Univ Erasmus Medical Ct | Method and culture medium for in vitro culturing of stem cells. |
JP6635505B2 (ja) * | 2013-11-01 | 2020-01-29 | 国立大学法人京都大学 | 新規軟骨細胞誘導方法 |
CN104630138B (zh) * | 2013-11-06 | 2017-11-14 | 陕西瑞盛生物科技有限公司 | 一种无血清软骨细胞培养液 |
CN103805562B (zh) * | 2014-01-13 | 2015-08-05 | 章毅 | 培养胎盘间充质干细胞的无血清培养基 |
US10435464B1 (en) * | 2014-09-05 | 2019-10-08 | Coherus Biosciences, Inc. | Methods for making recombinant proteins |
CA2977578A1 (en) * | 2015-03-04 | 2016-09-09 | Mesoblast International Sarl | Cell culture method for mesenchymal stem cells |
FR3035407B1 (fr) | 2015-04-23 | 2022-06-17 | Francais Du Sang Ets | Procede de conservation de cellules, tissus ou organes en hypothermie |
JP6905938B2 (ja) * | 2015-05-05 | 2021-07-21 | コーディ キメCody Kime | プライム型多能性幹細胞のナイーブ型多能性幹細胞への復帰 |
CN104830753B (zh) * | 2015-05-20 | 2018-07-10 | 广州赛莱拉干细胞科技股份有限公司 | 一种诱导多能干细胞培养基、应用及培养方法 |
WO2016187820A1 (zh) * | 2015-05-26 | 2016-12-01 | 中国科学院广州生物医药与健康研究院 | 一种人原代细胞培养基及其应用 |
CN105779385A (zh) * | 2016-03-31 | 2016-07-20 | 谷超 | 一种间充质干细胞培养试剂盒 |
US10959425B2 (en) | 2016-04-29 | 2021-03-30 | Hope Biosciences, Llc | Method of banking stem cells |
US10988731B2 (en) | 2016-04-29 | 2021-04-27 | Hope Biosciences, Llc | Formulation for storage, transportation, and delivery of protein rich conditioned medium |
US11111480B2 (en) | 2016-04-29 | 2021-09-07 | Hope Biosctences, Llc | Culture media for multipotent stem cells |
US10894947B1 (en) | 2016-04-29 | 2021-01-19 | Hope Biosciences, Llc | Method for generating protein rich conditioned medium |
CN105769740B (zh) * | 2016-05-11 | 2019-03-19 | 紫程瑞生会(北京)生物技术发展有限公司 | 一种人干细胞来源的生物美容原料的制备方法及其产品 |
CN106222136A (zh) * | 2016-08-08 | 2016-12-14 | 安徽惠恩生物科技股份有限公司 | 一种用于脂肪干细胞的培养基 |
CN106244527B (zh) * | 2016-08-29 | 2019-10-08 | 广东依浦赛生物科技有限公司 | 人源iPS干细胞体外定向分化为心肌细胞的试剂盒及方法 |
CN106906182B (zh) * | 2017-04-28 | 2020-10-09 | 北京赛斯达生物技术有限公司 | 一种间充质干细胞无血清培养基 |
CN106916784A (zh) * | 2017-05-04 | 2017-07-04 | 刘力伟 | 一种用于人间充质干细胞的无血清培养基及其应用 |
CN107119011A (zh) * | 2017-05-04 | 2017-09-01 | 刘力伟 | 一种用于扩增人间充质干细胞的培养基及其扩增方法 |
USD875967S1 (en) | 2017-07-14 | 2020-02-18 | Hope Biosciences, Llc | Container for storing biological material |
USD848022S1 (en) | 2017-07-14 | 2019-05-07 | Hope Biosciences, Llc | Container for storing biological material |
CN107345216B (zh) * | 2017-07-28 | 2020-11-06 | 暨南大学 | 一种脂肪干细胞培养基及其应用 |
WO2019103528A2 (ko) * | 2017-11-24 | 2019-05-31 | 주식회사 차바이오랩 | 무혈청 배지 조성물 |
KR20200025210A (ko) * | 2018-08-29 | 2020-03-10 | 주식회사 스템모어 | 모유두 세포의 배양용 조성물 및 이를 이용한 모유두 세포의 배양 방법 |
CN109022356A (zh) * | 2018-08-30 | 2018-12-18 | 丰泽康生物医药(深圳)有限公司 | 一种提高间充质干细胞向软骨细胞分化的无血清培养基 |
CN109554351A (zh) * | 2018-12-12 | 2019-04-02 | 山西医科大学 | Rspo1诱导骨髓间充质干细胞向心肌样细胞分化的应用 |
CN110016461A (zh) * | 2019-01-15 | 2019-07-16 | 深圳市第二人民医院 | 一种软骨细胞体外扩增方法 |
CN111808799B (zh) * | 2020-08-28 | 2021-07-13 | 河南昆仑精准医疗科技有限公司 | 一种多功能干细胞培养方法及所用培养基 |
CN112725259B (zh) * | 2021-01-07 | 2023-08-29 | 福州市皮肤病防治院 | 一种干细胞向汗腺样细胞分化的诱导培养基和诱导方法 |
CN113337457A (zh) * | 2021-06-01 | 2021-09-03 | 澳门大学 | 一种无血清调控干细胞的细胞状态的方法以及调节剂的应用 |
CN113512533A (zh) * | 2021-07-30 | 2021-10-19 | 创芯国际生物科技(广州)有限公司 | 一种高精密组织芯片的制备方法及应用 |
WO2023044443A1 (en) * | 2021-09-16 | 2023-03-23 | Life Technologies Corporation | Cell expansion methods and compositions for use therein |
US20230257714A1 (en) * | 2022-02-07 | 2023-08-17 | Smartcella Solutions Ab | Methods and compositions for generating human induced mesenchymal stem cells |
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US5908782A (en) | 1995-06-05 | 1999-06-01 | Osiris Therapeutics, Inc. | Chemically defined medium for human mesenchymal stem cells |
US7015037B1 (en) * | 1999-08-05 | 2006-03-21 | Regents Of The University Of Minnesota | Multiponent adult stem cells and methods for isolation |
JP2004521128A (ja) * | 2001-02-23 | 2004-07-15 | ワイス | Bmpによるヒト骨髄由来cd105+細胞の軟骨形成能 |
JP2006505248A (ja) * | 2002-06-07 | 2006-02-16 | イーエス・セル・インターナショナル・プライヴェート・リミテッド | 幹細胞における分化を制御する方法 |
US7723106B2 (en) | 2004-07-29 | 2010-05-25 | Food Industry Research & Development Institute | Stroma-free, serum-free, and chemically defined medium and method for ex vivo mononuclear cell expansion using the same |
US7955846B2 (en) * | 2004-05-17 | 2011-06-07 | The General Hospital Corporation | Compositions comprising female germline stem cells and methods of use thereof |
AU2006210955A1 (en) | 2005-01-31 | 2006-08-10 | Es Cell International Pte Ltd. | Directed differentiation of embryonic stem cells and uses thereof |
WO2007080919A1 (ja) * | 2006-01-13 | 2007-07-19 | Japan Science And Technology Agency | 動物細胞を無血清培養するための培地用添加剤、キット及びこれらの利用 |
US20070287176A1 (en) * | 2006-06-13 | 2007-12-13 | Alireza Rezania | Chorionic villus derived cells |
AU2008206884B2 (en) * | 2007-01-18 | 2012-07-05 | Glykos Finland Oy | Novel methods and reagents directed to production of cells |
EP2148569B1 (en) * | 2007-04-23 | 2014-07-16 | Stowers Institute for Medical Research | Methods and compositions for hematopoeitic stem cell self-renewal |
WO2009116951A2 (en) * | 2008-03-17 | 2009-09-24 | Agency For Science, Technology And Research | Microcarriers for stem cell culture |
KR20180108887A (ko) * | 2008-08-20 | 2018-10-04 | 안트로제네시스 코포레이션 | 단리된 태반 세포를 사용한 뇌졸중 치료 |
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CN103068969A (zh) | 2013-04-24 |
KR20130112028A (ko) | 2013-10-11 |
US20130089928A1 (en) | 2013-04-11 |
JP2013529917A (ja) | 2013-07-25 |
US20160281060A1 (en) | 2016-09-29 |
WO2011159359A4 (en) | 2012-04-19 |
MX2012014232A (es) | 2013-03-21 |
WO2011159359A2 (en) | 2011-12-22 |
WO2011159359A3 (en) | 2012-02-23 |
AU2011265723A1 (en) | 2013-01-10 |
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