EP2582788A4 - Serum-free chemically defined cell culture medium - Google Patents
Serum-free chemically defined cell culture mediumInfo
- Publication number
- EP2582788A4 EP2582788A4 EP11796094.8A EP11796094A EP2582788A4 EP 2582788 A4 EP2582788 A4 EP 2582788A4 EP 11796094 A EP11796094 A EP 11796094A EP 2582788 A4 EP2582788 A4 EP 2582788A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- serum
- culture medium
- cell culture
- chemically defined
- defined cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0655—Chondrocytes; Cartilage
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/36—Lipids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/105—Insulin-like growth factors [IGF]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/135—Platelet-derived growth factor [PDGF]
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/33—Insulin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
- C12N2501/415—Wnt; Frizzeled
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/80—Neurotransmitters; Neurohormones
- C12N2501/825—Serotonine (5-HT); Melatonine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US39784710P | 2010-06-17 | 2010-06-17 | |
PCT/US2011/001088 WO2011159359A2 (en) | 2010-06-17 | 2011-06-16 | Serum-free chemically defined cell culture medium |
Publications (2)
Publication Number | Publication Date |
---|---|
EP2582788A2 EP2582788A2 (en) | 2013-04-24 |
EP2582788A4 true EP2582788A4 (en) | 2014-03-19 |
Family
ID=45348785
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP11796094.8A Withdrawn EP2582788A4 (en) | 2010-06-17 | 2011-06-16 | Serum-free chemically defined cell culture medium |
Country Status (9)
Country | Link |
---|---|
US (2) | US20130089928A1 (en) |
EP (1) | EP2582788A4 (en) |
JP (1) | JP2013529917A (en) |
KR (1) | KR20130112028A (en) |
CN (1) | CN103068969A (en) |
AU (1) | AU2011265723A1 (en) |
BR (1) | BR112012032164A2 (en) |
MX (1) | MX2012014232A (en) |
WO (1) | WO2011159359A2 (en) |
Families Citing this family (42)
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JP5993113B2 (en) * | 2011-01-31 | 2016-09-14 | 株式会社 バイオミメティクスシンパシーズ | Human adipose tissue-derived mesenchymal stem cells for the treatment of Alzheimer's disease |
JP6238445B2 (en) * | 2011-10-27 | 2017-11-29 | 国立大学法人 東京医科歯科大学 | Colorectal epithelial stem cell isolation and culture technology, and colorectal epithelial transplantation technology using this |
WO2013165252A1 (en) * | 2012-05-03 | 2013-11-07 | Erasmus University Medical Center Rotterdam | Culturing of mesenchymal stem cells |
KR101523040B1 (en) * | 2012-06-26 | 2015-05-26 | 라정찬 | Method for Preparing High Concentration of Stem Cells |
CN103060264B (en) * | 2012-12-20 | 2015-01-21 | 上海市第十人民医院 | Stem cell culture medium and application thereof and stem cell cultivation method |
CN103255103B (en) * | 2013-04-17 | 2015-01-14 | 冯文峰 | Serum-free adipose tissue-derived mesenchymal stem cell culture medium |
CA2917684C (en) * | 2013-06-12 | 2021-07-20 | Shiseido Company, Ltd. | Serum-free medium containing pdgf for ds cells |
NL2011170C2 (en) * | 2013-07-15 | 2015-01-21 | Univ Erasmus Medical Ct | Method and culture medium for in vitro culturing of stem cells. |
KR102219743B1 (en) * | 2013-11-01 | 2021-02-23 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | Novel chondrocyte induction method |
CN104630138B (en) * | 2013-11-06 | 2017-11-14 | 陕西瑞盛生物科技有限公司 | A kind of serum-free cultured chondrocytes liquid |
CN103805562B (en) * | 2014-01-13 | 2015-08-05 | 章毅 | Cultivate the serum free medium of placenta mesenchyma stem cell |
US10435464B1 (en) * | 2014-09-05 | 2019-10-08 | Coherus Biosciences, Inc. | Methods for making recombinant proteins |
KR102635212B1 (en) * | 2015-03-04 | 2024-02-13 | 메조블라스트 인터내셔널 에스에이알엘 | Cell culture method for mesenchymal stem cells |
FR3035407B1 (en) | 2015-04-23 | 2022-06-17 | Francais Du Sang Ets | METHOD FOR PRESERVING CELLS, TISSUES OR ORGANS IN HYPOTHERMIA |
JP6905938B2 (en) * | 2015-05-05 | 2021-07-21 | コーディ キメCody Kime | Return of prime-type pluripotent stem cells to naive-type pluripotent stem cells |
CN104830753B (en) * | 2015-05-20 | 2018-07-10 | 广州赛莱拉干细胞科技股份有限公司 | A kind of induced multi-potent stem cell culture medium, application and cultural method |
CN105517672B (en) * | 2015-05-26 | 2019-06-21 | 中国科学院广州生物医药与健康研究院 | A kind of people's primitive cell culture base and its application |
CN105779385A (en) * | 2016-03-31 | 2016-07-20 | 谷超 | Mesenchymal stem cell culture kit |
US10894947B1 (en) | 2016-04-29 | 2021-01-19 | Hope Biosciences, Llc | Method for generating protein rich conditioned medium |
US10959425B2 (en) | 2016-04-29 | 2021-03-30 | Hope Biosciences, Llc | Method of banking stem cells |
US11111480B2 (en) | 2016-04-29 | 2021-09-07 | Hope Biosctences, Llc | Culture media for multipotent stem cells |
US10988731B2 (en) | 2016-04-29 | 2021-04-27 | Hope Biosciences, Llc | Formulation for storage, transportation, and delivery of protein rich conditioned medium |
CN105769740B (en) * | 2016-05-11 | 2019-03-19 | 紫程瑞生会(北京)生物技术发展有限公司 | A kind of preparation method and products thereof of the biological beauty raw material in human stem cell source |
CN106222136A (en) * | 2016-08-08 | 2016-12-14 | 安徽惠恩生物科技股份有限公司 | A kind of culture medium for fat stem cell |
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CN112322580B (en) * | 2017-04-28 | 2022-03-22 | 北京赛斯达生物技术有限公司 | Application of serum-free medium for mesenchymal stem cells |
CN106916784A (en) * | 2017-05-04 | 2017-07-04 | 刘力伟 | A kind of serum free medium and its application for human mesenchymal stem cell |
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WO2019103528A2 (en) * | 2017-11-24 | 2019-05-31 | 주식회사 차바이오랩 | Serum-free culture medium composition |
KR20200025210A (en) * | 2018-08-29 | 2020-03-10 | 주식회사 스템모어 | Composition for culturing dermal papilla cells and the method for culturing dermal papilla cells using the same |
CN109022356A (en) * | 2018-08-30 | 2018-12-18 | 丰泽康生物医药(深圳)有限公司 | A kind of serum free medium improving mesenchymal stem cells into chondrocytes differentiation |
CN109554351A (en) * | 2018-12-12 | 2019-04-02 | 山西医科大学 | The application of Rspo1 inducing bone mesenchymal stem cell Cardiocytes differentiation |
CN110016461A (en) * | 2019-01-15 | 2019-07-16 | 深圳市第二人民医院 | A kind of cartilage cell's amplification in vitro method |
CN111808799B (en) * | 2020-08-28 | 2021-07-13 | 河南昆仑精准医疗科技有限公司 | Multifunctional stem cell culture method and culture medium used by same |
CN112725259B (en) * | 2021-01-07 | 2023-08-29 | 福州市皮肤病防治院 | Induction medium and induction method for differentiation of stem cells into sweat gland-like cells |
CN113337457A (en) * | 2021-06-01 | 2021-09-03 | 澳门大学 | Method for serum-free regulation of cell state of stem cells and application of regulator |
CN113512533A (en) * | 2021-07-30 | 2021-10-19 | 创芯国际生物科技(广州)有限公司 | Preparation method and application of high-precision tissue chip |
WO2023044443A1 (en) * | 2021-09-16 | 2023-03-23 | Life Technologies Corporation | Cell expansion methods and compositions for use therein |
WO2023148556A1 (en) * | 2022-02-07 | 2023-08-10 | Smartcella Solutions Ab | Methods and compositions for generating human induced mesenchymal stem cells |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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US20060010508A1 (en) * | 2004-05-17 | 2006-01-12 | Tilly Jonathan L | Compositions comprising female germline stem cells and methods of use thereof |
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AU2006210955A1 (en) | 2005-01-31 | 2006-08-10 | Es Cell International Pte Ltd. | Directed differentiation of embryonic stem cells and uses thereof |
US9074176B2 (en) * | 2006-01-13 | 2015-07-07 | Two Cells Co., Ltd. | Culture medium additive for use in serum-free culturing of animal cell, kit and use thereof |
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KR20130112028A (en) | 2013-10-11 |
CN103068969A (en) | 2013-04-24 |
BR112012032164A2 (en) | 2017-11-28 |
US10287550B2 (en) | 2019-05-14 |
WO2011159359A2 (en) | 2011-12-22 |
WO2011159359A3 (en) | 2012-02-23 |
WO2011159359A4 (en) | 2012-04-19 |
EP2582788A2 (en) | 2013-04-24 |
US20130089928A1 (en) | 2013-04-11 |
AU2011265723A1 (en) | 2013-01-10 |
MX2012014232A (en) | 2013-03-21 |
US20160281060A1 (en) | 2016-09-29 |
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