UA41863C2 - Спосіб одержання гетерологічного поліпептиду в еукаріотичних мікроорганізмах - Google Patents

Спосіб одержання гетерологічного поліпептиду в еукаріотичних мікроорганізмах

Info

Publication number
UA41863C2
UA41863C2 UA4203156A UA4203156A UA41863C2 UA 41863 C2 UA41863 C2 UA 41863C2 UA 4203156 A UA4203156 A UA 4203156A UA 4203156 A UA4203156 A UA 4203156A UA 41863 C2 UA41863 C2 UA 41863C2
Authority
UA
Ukraine
Prior art keywords
heterologous polypeptide
signal peptide
eukaryotic microorganisms
bari
lys
Prior art date
Application number
UA4203156A
Other languages
English (en)
Russian (ru)
Inventor
Вівіан Л. Маккей
Вивиан Л. Маккей
Original Assignee
Займодженетікс Інк.
ЗАЙМОДЖЕНЕТИКС Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Займодженетікс Інк., ЗАЙМОДЖЕНЕТИКС Инк. filed Critical Займодженетікс Інк.
Publication of UA41863C2 publication Critical patent/UA41863C2/uk

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Спосіб одержання гетерологічного поліпептиду в еукаріотичних мікроорганізмах, що передбачає конструювання рекомбінантної плазмідної ДНК, що містить промотор, послідовність, що кодує сигнальний пептид і структурний ген, трансформацію отриманої рекомбінантної ДНК штамів-реципієнтів, культивування трансформантів, виділення і очищення цільового продукту. Конструюють рекомбінантну плазмідну ДНК, що містить промотор BARI Saccharomyces cerevisiae або промотор алкогольдегідрогенази I S.cerevisiae, частину послідовності гена BARI, що кодує сигнальний пептид, і місце відщеплювання сигнального пептиду КЕХ2 від гетерологічного поліпептиду, яке являє собою Arg-Arg або Lys-Lys.
UA4203156A 1985-10-25 1986-10-20 Спосіб одержання гетерологічного поліпептиду в еукаріотичних мікроорганізмах UA41863C2 (uk)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US79130585A 1985-10-25 1985-10-25
PCT/US1986/002198 WO1987002670A1 (en) 1985-10-25 1986-10-20 Method of using bar1 for secreting foreign proteins

Publications (1)

Publication Number Publication Date
UA41863C2 true UA41863C2 (uk) 2001-10-15

Family

ID=25153298

Family Applications (1)

Application Number Title Priority Date Filing Date
UA4203156A UA41863C2 (uk) 1985-10-25 1986-10-20 Спосіб одержання гетерологічного поліпептиду в еукаріотичних мікроорганізмах

Country Status (13)

Country Link
EP (1) EP0243465A1 (uk)
JP (1) JP2523562B2 (uk)
CN (1) CN1027179C (uk)
AU (2) AU6543286A (uk)
CA (1) CA1316133C (uk)
CZ (1) CZ284251B6 (uk)
DK (1) DK320287D0 (uk)
FI (1) FI872801A (uk)
HU (1) HU206897B (uk)
IE (1) IE63822B1 (uk)
SK (1) SK279041B6 (uk)
UA (1) UA41863C2 (uk)
WO (1) WO1987002670A1 (uk)

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Also Published As

Publication number Publication date
CZ776486A3 (en) 1996-09-11
DK320287A (da) 1987-06-23
JP2523562B2 (ja) 1996-08-14
IE862804L (en) 1987-04-25
SK776486A3 (en) 1998-06-03
CN1027179C (zh) 1994-12-28
HUT43624A (en) 1987-11-30
EP0243465A1 (en) 1987-11-04
AU676132B2 (en) 1997-03-06
AU7400391A (en) 1991-07-18
DK320287D0 (da) 1987-06-23
CZ284251B6 (cs) 1998-10-14
FI872801A0 (fi) 1987-06-24
HU206897B (en) 1993-01-28
JPS63501614A (ja) 1988-06-23
AU6543286A (en) 1987-05-19
FI872801A (fi) 1987-06-24
CN86107554A (zh) 1987-08-26
IE63822B1 (en) 1995-06-14
CA1316133C (en) 1993-04-13
SK279041B6 (sk) 1998-06-03
WO1987002670A1 (en) 1987-05-07

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